Title of Invention	"A PROCESS OF PREPARING CONSORTIUM OF BACTERIOPHAGES USEFUL FOR CONTROLLING LUMINOUS BACTERIAL DISEASE IN SHRIMP LARVAE".
Abstract	An environment friendly technology for management (both prevention and therapy) of luminous bacterial disease in shrimp using novel vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) characterized by selection of naturally isolated bacteriophages vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) based on their morphology, growth cycle, lytic spectrum and absence of virulence genes, mass production of these bacteriophages by propagating on their respective host grown as a lawn culture on TSAS, wherein the host culture is grown on TSBS for 12h and 4ml host culture and 1ml phage suspension are mixed and spread on the surface of TSAS Agar in Roux bottles, incubating at room temperature such as 28°C for 5-6 hours, harvesting the phages in phage buffer, determining the phase titre by a soft agar overlay technique, wherein the soft agar tubes are maintained at 48-50°C in a water bath, serially diluting the phage suspensions in phage buffer, followed by mixing hundred micro liter for each phage dilution with 1 ml of 8h old host culture in TSBS and then adding to 5 ml of molten soft agar intubes, wherein the mixture is rolled between palms to enhance good mixing, overlaying on TSAS plates wherein the plates are incubated overnight at ambient 28+l°C temperature after solidification, Counting the plaques and expressing as plaque forming units/milliliter.

Title of Invention

"A PROCESS OF PREPARING CONSORTIUM OF BACTERIOPHAGES USEFUL FOR CONTROLLING LUMINOUS BACTERIAL DISEASE IN SHRIMP LARVAE".

Abstract

An environment friendly technology for management (both prevention and therapy) of luminous bacterial disease in shrimp using novel vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) characterized by selection of naturally isolated bacteriophages vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) based on their morphology, growth cycle, lytic spectrum and absence of virulence genes, mass production of these bacteriophages by propagating on their respective host grown as a lawn culture on TSAS, wherein the host culture is grown on TSBS for 12h and 4ml host culture and 1ml phage suspension are mixed and spread on the surface of TSAS Agar in Roux bottles, incubating at room temperature such as 28°C for 5-6 hours, harvesting the phages in phage buffer, determining the phase titre by a soft agar overlay technique, wherein the soft agar tubes are maintained at 48-50°C in a water bath, serially diluting the phage suspensions in phage buffer, followed by mixing hundred micro liter for each phage dilution with 1 ml of 8h old host culture in TSBS and then adding to 5 ml of molten soft agar intubes, wherein the mixture is rolled between palms to enhance good mixing, overlaying on TSAS plates wherein the plates are incubated overnight at ambient 28+l°C temperature after solidification, Counting the plaques and expressing as plaque forming units/milliliter.

Full Text	WE CLAIM; 1. An environment friendly technology for management (both prevention and therapy) of luminous bacterial disease in shrimp using novel vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) characterized by:- i) selection of naturally isolated bacteriophages vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) based on their morphology, growth cycle, lytic spectrum and absence of virulence genes, ii) mass production of these bacteriophages by propagating on their respective host grown as a lawn culture on TSAS, wherein the host culture is grown on TSBS for 12h and 4ml host culture and 1ml phage suspension are mixed and spread on the surface of TSAS Agar in Roux bottles, iii) incubating at room temperature such as 28°C for 5-6 hours, iv) Harvesting the phages in phage buffer, v) Determining the phase titre by a soft agar overlay technique, wherein the soft agar tubes are maintained at 48-50°C in a water bath, vi) Serially diluting the phage suspensions in phage buffer, followed by mixing hundred micro liter for each phage dilution with 1 ml of 8h old host culture in TSBS and then adding to 5 ml of molten soft agar intubes, wherein the mixture is rolled between palms to enhance good mixing, vii) Overlaying on TSAS plates wherein the plates are incubated overnight at ambient 28+l°C temperature after solidification, viii) Counting the plaques and expressing as plaque forming units/milliliter. 2. The technology for mass production of bateriophages as claimed in claim 1, wherein the phage buffer used is 5.8 g/L Nacl, 2.0 g/L MgSC-4, 50 ml/L IM Tris pH 7.5 and 5 ml/L of 2% pre-sterilized gelatin. 3. The technology for mass production of bateriophages as claimed in claim 1, wherein the soft agar used is 15 g/L Tryptone, 5 g/L yeast extract, 10g/L Nacl, 1 g/L Mgcl2, IM 10ml/L Cacl2, 3g/L Agar.

Full Text

WE CLAIM;
1. An environment friendly technology for management (both prevention and therapy) of luminous bacterial disease in shrimp using novel vibrio harveyi bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) characterized by:-
i) selection of naturally isolated bacteriophages vibrio harveyi
bacteriophage-8 (viha 8) and vibrio harveyi bacteriophage-10 (viha 10) based on their morphology, growth cycle, lytic spectrum and absence of virulence genes,
ii) mass production of these bacteriophages by propagating on their respective host grown as a lawn culture on TSAS, wherein the host culture is grown on TSBS for 12h and 4ml host culture and 1ml phage suspension are mixed and spread on the surface of TSAS Agar in Roux bottles,
iii) incubating at room temperature such as 28°C for 5-6 hours, iv) Harvesting the phages in phage buffer,
v) Determining the phase titre by a soft agar overlay technique, wherein the soft agar tubes are maintained at 48-50°C in a water bath,
vi) Serially diluting the phage suspensions in phage buffer, followed by mixing hundred micro liter for each phage dilution with 1 ml of 8h old host culture in TSBS and then adding to 5 ml of molten soft agar intubes, wherein the mixture is rolled between palms to enhance good mixing,
vii) Overlaying on TSAS plates wherein the plates are incubated overnight at ambient 28+l°C temperature after solidification,
viii) Counting the plaques and expressing as plaque forming units/milliliter.
2. The technology for mass production of bateriophages as claimed in
claim 1, wherein the phage buffer used is 5.8 g/L Nacl, 2.0 g/L
MgSC-4, 50 ml/L IM Tris pH 7.5 and 5 ml/L of 2% pre-sterilized
gelatin.
3. The technology for mass production of bateriophages as claimed in claim 1, wherein the soft agar used is 15 g/L Tryptone, 5 g/L yeast extract, 10g/L Nacl, 1 g/L Mgcl2, IM 10ml/L Cacl2, 3g/L Agar.

Documents:

1818-del-2006-Abstract-(11-09-2012).pdf

1818-DEL-2006-Abstract-(17-09-2012).pdf

1818-DEL-2006-Abstract-(18-05-2012).pdf

1818-DEL-2006-Abstract-(20-10-2010).pdf

1818-DEL-2006-Abstract-(26-05-2011).pdf

1818-del-2006-Claims-(11-09-2012).pdf

1818-DEL-2006-Claims-(17-09-2012).pdf

1818-DEL-2006-Claims-(18-05-2012).pdf

1818-DEL-2006-Claims-(20-10-2010).pdf

1818-DEL-2006-Claims-(26-05-2011).pdf

1818-del-2006-Correspondence Others-(05-03-2013).pdf

1818-del-2006-Correspondence Others-(11-09-2012).pdf

1818-DEL-2006-Correspondence Others-(18-05-2012).pdf

1818-DEL-2006-Correspondence Others-(26-05-2011).pdf

1818-DEL-2006-Correspondence-Others-(17-09-2012).pdf

1818-DEL-2006-Correspondence-Others-(20-10-2010).pdf

1818-del-2006-Correspondence-Others-(24-06-2013).pdf

1818-del-2006-Correspondence-Others-(25-10-2012).pdf

1818-DEL-2006-Correspondence-Others-(28-09-2012).pdf

1818-del-2006-Correspondence-Others-(29-08-2013).pdf

1818-del-2006-correspondence-others-1.pdf

1818-del-2006-correspondence-po.pdf

1818-del-2006-correspondesce-others.pdf

1818-del-2006-Description (Complete)-(11-09-2012).pdf

1818-DEL-2006-Description (Complete)-(17-09-2012).pdf

1818-DEL-2006-Description (Complete)-(18-05-2012).pdf

1818-DEL-2006-Description (Complete)-(20-10-2010).pdf

1818-del-2006-description (provisional).pdf

1818-DEL-2006-Form-1-(17-09-2012).pdf

1818-DEL-2006-Form-1-(26-05-2011).pdf

1818-del-2006-form-1.pdf

1818-del-2006-Form-13-(29-08-2013).pdf

1818-del-2006-form-18.pdf

1818-DEL-2006-Form-2-(17-09-2012).pdf

1818-DEL-2006-Form-2-(20-10-2010).pdf

1818-DEL-2006-Form-2-(26-05-2011).pdf

1818-del-2006-form-2.pdf

1818-del-2006-gpa.pdf

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Patent Number

260063

Indian Patent Application Number

1818/DEL/2006

PG Journal Number

14/2014

Publication Date

04-Apr-2014

Grant Date

31-Mar-2014

Date of Filing

11-Aug-2006

Name of Patentee

DEPARTMENT OF BIOTECHNOLOGY

Applicant Address

BLOCK 2, 7TH FLOOR, CGO COMPLEX, LODHI ROAD,, NEW DELHI-110003

Inventors:

#	Inventor's Name	Inventor's Address
1	DR. INDRANI KARUNASAGAR	DEPTT. OF MICROBIOLOGY, KARNATKA VERTERINARY ANIMAL AND FISHERIES SCIENCE UNIVERSITY, COLLEGE OF FISHERIES, MANGLORE-575002, INDIA
2	DR. IDDYA KARUNASAGAR	DEPTT. OF MICROBIOLOGY, KARNATAKA VERTERINARY ANIMAL AND FISHERIES SCIENCE UNIVERSITY, COLLEGE OF FISHERIES, MANGLORE-575002, INDIA

PCT International Classification Number

C12N1/00

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA