Title of Invention | A FORMULATION FOR THE STABILIZATION OF ALLERGENIC EXTRACTS USED IN DIAGNOSIS AND THERAPY OF ALLERGY. |
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Abstract | The invention discloses a formulation, which stabilizes allergen extracts, increases the shelf life and maintains the biological potency of the said extract when stored at 4°C. The formulation consists of a combination of preservatives, which enhances the shelf life of the extract as compared to the extracts without preservatives and those preserved with individual preservatives. |
Full Text | We Claim 1. A synergistic formulation for stabilizing protease rich allergen extract wherein the said formulation comprising 0.025M-0.1M epsilon-aminocaproic acid (EACA) and 0.25M-0.75M sucrose. 5-10% glycerol, l-5µM pepstatinA and 2-10 µM aprotnin. 2. A synergistic formulation as claimed in claim 1 wherein a preferable concentration of epsilon-aminocaproic acid (EACA) is 0.05M and a preferable concentration of sucrose is 0.25M 3. A method for stabilizing the protease rich allergen extract as claimed in claim 1 wherein the said method comprises of following steps: (a) preparing the allergen extract by extracting the grounded defatted cockroaches inh aqueous buffer at neutral pH followed by lyophilization; (b) storing the allergen extract as obtained in step (a) with the said formulation as claimed in claim 1, at temperature ranging between 4°-37°C; (c) evaluating the stabilized allergen extracts for various immuno-biochemical properties and allergy diagnosis potential. 4. A method as claimed in claim 3 wherein the buffer used for preparing the allergen extract is selected from the group containing Tris buffer saline, phosphate buffer, phosphate buffer saline. 5. A method as claimed in claim 3 wherein the pH of the buffer for allergen extract is in range of 6.8-7.5. 6. A method as claimed in claim 3 wherein the stability of the extract after treating with the said formulation is evaluated for immuno-biochemical properties such as protein stability, imunoblotting, ELISA, ELISA inhibition and intradermal test and compared to the standard lyophilizes extract stored at -70°C. |
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2192-DEL-2006-Claims-(01-08-2012).pdf
2192-DEL-2006-Correspondence Others-(01-08-2012).pdf
2192-del-2006-correspondence-others-1.pdf
2192-del-2006-description (provisional).pdf
Patent Number | 256122 | ||||||||||||
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Indian Patent Application Number | 2192/DEL/2006 | ||||||||||||
PG Journal Number | 19/2013 | ||||||||||||
Publication Date | 10-May-2013 | ||||||||||||
Grant Date | 05-May-2013 | ||||||||||||
Date of Filing | 05-Oct-2006 | ||||||||||||
Name of Patentee | COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH | ||||||||||||
Applicant Address | ANUSANDHAN BHAWAN, RAFI MARG, NEW DELHI - 110001, INDIA | ||||||||||||
Inventors:
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PCT International Classification Number | C40B40/08 | ||||||||||||
PCT International Application Number | N/A | ||||||||||||
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