Title of Invention	IMPROVED BEVERAGE
Abstract	The present invention provides a beverage having an energy content of less than 17 kJ per 100 g, wherein the beverage comprises catechins in amount (C) of from 0.04 to 0.4% by weight of the beverage, and non-nutritive sweetener in an amount satisfying the following condition: 34 < S/C < 115, wherein S is the sweetness of the non-nutritive sweetener in terms of sucrose.

Full Text

FORM - 2 THE PATENTS ACT, 1970 (39 of 1970) & The Patents Rules, 2003 COMPLETE SPECIFICATION (See Section 10 and Rule 13) IMPROVED BEVERAGE HINDUSTAN UNILEVER LIMITED, a company incorporated under the Indian Companies Act, 1913 and having its registered office at 165/166, Backbay Reclamation, Mumbai -400 020, Maharashtra, India The following specification particularly describes the invention and the manner in which it is to be performed WO 2008/012147 PCT/EP2007/056079 IMPROVED BEVERAGE Technical Field of the Invention The present invention relates to beverages. More particularly the 5 invention relates to beverages containing catechins, such as tea-based beverages. Background of the Invention Green tea is a popular beverage which has been consumed in China 10 and Japan for many hundreds of years. Recently, extensive laboratory research and epidemiologic studies have shown that compounds present in green tea (particularly catechins) may reduce the risk of a variety of illnesses. Furthermore, catechins have been shown to suppress accumulation of visceral fat and so 15 may be useful in controlling bodyweight and bodyshape (see, for example, T. Nagao et al., "Tea Catechins Suppress Accumulation of Body Fat in Humans', J. Oleo. Sci., 2001, _50(9), pp.717-728) . These studies, along with the increasing complexity of the consumer's palate have led to growth in the consumption of green 20 tea, even in markets (such as the USA and Western Europe) where there is no tradition of green tea consumption. Although, some of the health benefits of tea may be apparent at consumption rates as low as three cups per day (see, for example, 25 U.Peters et al.r "Does tea affect cardiovascular disease? A meta¬analysis.", American Journal of Epidemiology, 2001, 154, pp.495-503), many individuals do not even achieve this modest consumption rate on a long term basis. 30 There have been several attempts to provide beverages with enhanced levels of catechins. WO 2008/012147 PCT/£P2007/056079 European patent EP 0 762 836 B discloses a beverage whereby cellular hydration and drinkability are enhanced by the combination of green tea solids with selected levels and types of electrolytes and carbohydrates. Unfortunately, however, the 5 presence of significant levels of carbohydrates in the beverage may compromise certain benefits delivered by the catechins. in particular, the high energy contribution of sugars would tend to counteract any benefits of the catechins in terms of controlling bodyweight and/or bodyshape. 10 European patent application EP 1 297 749 A discloses a beverage having a concentrated or purified tea extract incorporated therein, containing catechins from 0.092 to 0.5 wt.% and quinic acid in a specific weight ratio with respect to the catechins. 15 The beverage is said to have an improved taste and does not cause an unpleasant aftertaste which will otherwise remain after bitterness or astringency peculiar to catechins is alleviated by the addition of a sweetener. However, the beverages disclosed therein require the addition of quinic acid which itself may 20 bring unwanted taste to the beverage. Furthermore, the beverages are still unpleasantly bitter, especially for consumers used to tea-based beverages having relatively low levels of catechins, such as black tea. 2 5 Thus we have recognised that there is a need to provide a beverage which delivers the benefits of high levels of catechins, especially in respect of bodyweight and/or shape management, which beverage has improved taste, especially in respect of bitterness. 30 We have found that such a need may be met by providing a beverage having a specific composition. WO 2008/012147 PCT/EP2007/056079 Tests and Definitions BEVERAGE As used herein the term "beverage" refers to a substantially aqueous drinkable composition suitable for human consumption. 5 Preferably the beverage comprises at least 85% water by weight of the beverage, more preferably at least 90% and most preferably from 95 to 99.9%. TEA SOLIDS 10 As used herein, the term "tea solids" refers to dry material extractable from the leaves of the plant Camellia sinensis var. sinensis and/or Camellia sinensis var. assamica. The leaves may have been subjected to a so-called "fermentation" step wherein they are oxidised by certain endogenous enzymes that are released 15 during the early stages of "black tea" manufacture. This oxidation may even be supplemented by the action of exogenous enzymes such as oxidases, laccases and peroxidases. Alternatively the leaves may have been partially fermented ("oolong" tea) or substantially unfermented ("green tea"). 20 TEA-BASED BEVERAGE As used herein, the term "tea-based beverage" refers to a beverage comprising at least 0.01% by weight tea solids by weight of the beverage. Preferably the tea-based beverage comprises from 25 0.04 to 3% tea solids, more preferably from 0.06 to 2%, most preferably from 0.1 to 1%. CATECHINS As used herein the term "catechins" is used as a generic term for 30 catechin, gallocatechin, catechin gallate, gallocatechin gallate, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, and mixtures thereof. WO 2008/012147 PCT/EP2007/056079 DETERMINATION OF CATECHINS AND CAFFEINE IN A BEVERAGE The amounts of catechins and caffeine in a beverage are determined simultaneously by reverse-phase HPLC as follows: 10 Sample Preparation 9 ml of the beverage are taken and 1.12 ml of acetonitrile added, along with 1.12 ml of a solution of 2.5 mg/ml EDTA and 2.5 mg/ml ascorbic acid in distilled water. The resulting solution is then decanted into microcentrifuge tubes and centrifuged at a relative centrifugal force of 14000 g for 10 minutes. 15 HPLC Analysis conditions Column: Luna Phenyl hexyl 5jir 250 x 4.60 mm Flow rate: 1 ml/min Oven temperature: 30°C Solvents: A: 2% acetic acid in acetonitrile B: 2% acetic acid and 0.02 mg/ml EDTA in water 20 Injection volume: 10 [Xl 25 Gradient: Time % Solvent A % Solvent B Step 0 to 10 min 5 95 Isocratic 10 to 40 min 5-18 95 - 85 Linear gradient 40 to 50 min 18 82 Isocratic 50 to 55 min 50 50 Wash 55 to 75 min 5 95 Isocratic 30 Quantification: Peak area relative to a calibration curve constructed daily. Calibration curve is constructed from caffeine and the concentration of catechins is calculated using the relative response factors of the individual catechins to caffeine WO 2008/012147 PCT/EP2007/056079 (from the ISO catechin method - ISO/CD 14502-2). Individual caffeine standards (Sigma, Poole, Dorset, UK) are used as peak identification markers. 5 SUGARS As used herein the term "sugars" refers exclusively to digestible mono- and di-saccharides. This includes any sugars from tea solids, milk and fruit juice. 10 TOTAL ENERGY CONTENT The total energy content (E) of a beverage is defined as the sum of the metabolizable energy of the ingredients present within 100 g of the beverage. It is calculated using Equation 1: 15 j 20 wherein Cj is the mass of ingredient j in 100 g of the beverage and /j is the calorie conversion factor for the ingredient j. For digestible saccharides and proteins, fj is typically 16.7 kJ 25 (4.0 kcal) per g. For fats, f-j is 37.7 kJ (9.0 kcal) per g. Calorie conversion factors are readily available from ingredient manufacturers. Alternatively, the factors may be determined from analysis and comparison of the composition and heats of 30 combustion of dietary material with those of excreted material (see D.A.T. Southgate and J.V.G.A. Durnin, "Calorie conversion factors. An experimental reassessment of the factors used in the calculation of the energy value of human diets.", British Journai of Nutrition, 1970, 24, pp. 517-535). WO 2008/012147 PCT/EP2007/056079 NON-NUTRITIVE SWEETENER Non-nutritive sweeteners consist of intense sweeteners, sugar alcohols and mixtures thereof. Intense sweeteners are those materials with a relative sweetness of at least 10, more 5 preferably from 30 to 30000. The intense sweeteners include aspartame, saccharin, acesulfame K, alitame, thaumatin, cyclamate, glycyrrhizin, stevioside, neohesperidine, sucralose, monellin, neotame, mogroside V, MGGR, periandrin I-V, rebaudioside A, stevia extract and mixtures thereof. Sugar 0 alcohols include HSH (hydrogenated starch hydrosylate - also known as polyglycitol), eythritol, arabitol, glycerol, xylitol, sorbitol, mannitol, lactitol, maltitol, isomalt, palatinit and mixtures thereof. 5 SWEETNESS As defined herein, the relative sweetness, Ri, refers to the sweetness of substance i relative to the sweetness of an equivalent weight of sucrose (i.e. sucrose has a relative sweetness of 1) . This is a common means of expressing sweetness 0 in the food industry. The relative sweetness of certain non-nutritive sweeteners is given in Table 1. The sweetness in terms of sucrose. Si, of non-nutritive sweetener i present in an amount of mi (percent by weight of the beverage), 5 is the product of the relative sweetness and the amount, i.e. S* = Riirii. Thus, for a mixture of non-saccharide sweeteners, the sweetness in terms of sucrose (S) is defined by Equation 2: WO 2008/012147 PCT/EP2007/056079 Summary of the Invention In a first aspect, the present invention provides a beverage having a total energy content of less than 17 kJ per 100 g, wherein the beverage comprises catechins in amount (C) of from 5 0.04 to 0.4% by weight of the beverage, and non-nutritive sweetener in an amount such that the sweetness (S) of the non-nutritive sweetener in terms of sucrose is less than 7% by weight of the beverage and satisfies the following condition: 34 10 We have found that such beverages provide a well-balanced flavour in terms of sweetness aha- bitterness. It is preferred that the ratio (S/C) is less than 105, more preferably less than 95 and most preferably less than 85. Preferably also, the ratio {S/C) is 15 at least 37, more preferably at least 40 and most preferably at least 45. We have found that it is possible for the beverages of the invention to have a well-balanced flavour even at relatively low 20 levels of sweetness. Thus the sweetness (S) is less than 7 and preferably less than 5% by weight of the beverage. Preferably also, the sweetness is at least 1, more preferably at least 2 and most preferably at least 3% by weight of the beverage. 25 Surprisingly, we have also found that the beverages of the present invention have a well-balanced flavour even in the absence of bitter-masking agents such as quinic acid. Thus the beverage of the present invention is preferably free of added quinic acid. WO 2008/012147 PCT/EP2007/056079 To maximise the health benefits of the beverage, it is preferred that the amount of catechins (C) is at least 0.05%, more preferably at least 0.06% by weight of the beverage. To minimise bitterness it is preferred that the amount of catechins is less 5 than 0.3%, more preferably less than 0.2% by weight of the beverage. The low energy content of the beverage allows for delivery of the health benefits of catechins without providing an unhealthy 10 amount of calories. It is preferred that the total energy content is less than 5 kJ per 100 g, more preferably less than 2 kJ per 100 g and most preferably from 0.0001 to 1 kJ per 100 g. Because the sweetness of the beverage is primarily provided by 15 the non-nutritive sweetener it is preferred that the beverage comprises sugars in an amount of less than 0.25% by weight of the beverage, more preferably less than 0.1% and most preferably from 0.001 to 0.05%. 20 The concentration of non-nutritive sweetener will depend on the amount of catechins and the relative sweetness of the sweetener as set forth above; Typically, however, the beverage will comprise non-nutritive sweetener in an amount of between 0.00001 to 10% by weight of the beverage, more preferably from 0.001 to 25 1% and most preferably from 0.01 to 0.1%. In order to minimise the energy contribution of the sweetener, it is preferred that the non-nutritive sweetener comprises intense sweetener. Preferably the non-nutritive sweetener comprises at 30 least 50% intense sweetener by weight of the non-nutritive sweetener, more preferably at least 75% and most preferably from 90 to 100%. The most preferred intense sweeteners, owing to their WO 2008/012147 PCT/EP2OO7/O56079 well-rounded flavour, are sucralose, aspartame, acesulfame K, or a mixture thereof. We have found that the said ratio of sweetener to catechins is 5 particularly well-suited for delivering a tea-based beverage that has a refreshing tea flavour but is not too bitter. Thus it is preferred that the beverage is a tea-based beverage, preferably a green tea-based beverage. 10 From a standpoint of microbial stability and taste, it is preferred that the beverage has an acid pH. In particular, the pH (at 20°C) may be from 2 to 5, more preferably from 2.5 to 4.5, most preferably from 3 to 4. 15 Suitable acidulants include organic acids such as citric, malic, tartaric, ascorbic, salts thereof and mixtures thereof. A particularly well-balanced flavour may be provided when the acid comprises malic acid and/or a salt thereof. Mixtures of citric acid (and/or its salt), malic acid (and/or its salt), and 20 ascorbic acid (and/or its salt) also provide a good flavour. Typically, the concentration of the organic acid and/or salt in the beverage will be from 0.001 to 1%, more preferably from 0.01 to 0.5%. 25 The beverage preferably comprises flavour. Suitable flavours include natural and synthetic fruit flavours, and/or natural or synthetic herb flavours. Examples of fruit flavours include apple, peach, pear, lemon, lime, mandarin, grapefruit, cranberry, orange, strawberry, grape, kiwi, pineapple, passion fruit, mango, 30 guava, raspberry and cherry. Examples of herb flavours include jasmine, chamomile, rose, mint, hawthorn, chrysanthemum, osmanthus,. hibiscus, elderflower and verbena. Surprisingly, we WO 2008/012147 PCT/EP2007/056079 have found that pineapple flavour (natural or synthetic) is particularly effective at masking the bitterness of catechins, therefore it is particularly preferred that the flavour comprises pineapple flavour. The pineapple flavour may be natural or 5 synthetic. Preferably the pineapple flavour comprises 2-propenyl hexanoate. The amount of flavour is preferably between 0.001 and 0.5% by weight of the beverage, more preferably from 0.05 to 0.19%. 10 The beverage may additionally or alternatively comprise a weight management active. Suitable actives include biotin, pantothenic acid (vitamin B5), vitamin B6, niacin, magnesium, yerba mate extract, guarana extract, hydroxycitric acid and mixtures thereof. 15 In a most preferred embodiment, the beverage comprises caffeine in an amount of between 0.001 to 0.1% by weight of the beverage, more preferably from 0.005 to 0.05%. Caffeine is particularly preferred as it has been shown to assist in body management, 20 especially in weight control and/or control of body shape. The beverage of this invention may be used as a medicament or in the preparation of a medicament. In particular, the beverage may be used to provide any of the benefits associated with 25 consumption of catechins such as treating and/or preventing cancer; and/or treating and/or preventing cardio-vascular disease. It is particularly preferred to use the beverage for controlling the bodyweight and/or shape of an individual. For example, the beverage may be used in a method of providing at 30 least one of these benefits to an individual, the method comprising administering to the individual the beverage. Preferably the beverage is administered orally. WO 2008/012147 PC17EP2007/056079 Detailed Description The present invention will be further described with reference to the following example. 5 EXAMPLE 1 Five beverage products were made having the formulations given in Table 2. TABLE 2 Ingredient Green tea powder* 2.500 2.500 2.500 2.500 3.840 Tri-sodium citrate 0.500 0.500 0.500 0.500 0.300 Citric acid 1.000 1.000 1.000 1.000 0.900 Malic acid 0.500 0.500 0.500 0.500 0.300 Ascorbic acid 0.200 0.200 0.200 0.200 0.200 Sucralose 0.120 0.080 0.060 0.040 0.100 Acesulfame K C.050 0.035 0.025 0.017 0.044 Flavour* * 1.150 1.150 1.150 1.150 1.150 Deionised Water To 1000 To 1000 To 1000 To 1000 To 1000 Properties C - Catechins (wt.%) 0.07 0.07 0.07 0.07 0.11 S ~ Sweetness (wt.%) 7.0 4.7 3.5 2.3 5.9 Caffeine (wt.%) 0.013 0.013 0.013 0.013 0.021 PH 3.5 3.5 3.5 3.5 3.5 1 0 *CeytealK from Premium Exports Ceylon Ltd (Sri Lanka). **Elderflower and Virbena. WO 2008/012147 PCT/EP200 7/056079 The products were made using the following protocol {all dissolution was performed at 20°C) : 1. Place half of the water in a glass beaker of the appropriate size. 5 2. Dissolve the green tea powder and tri sodium citrate together in a small amount of water and add to the glass beaker. 3. Dissolve flavours and sucralose in a small amount of water and add to the beaker. 3. Dissolve' the acesulfame K in a small amount of water and add 10 to the beaker. 4. Dissolve all the acids together (citric, malic and ascorbic) in a small amount of water and add to the beaker. 5. Add water to make up to the desired mass. 6. Fill the resulting solution into 330 ml cans and seal. 15 7. Pasteurise the cans at 70°C for 10 minutes in a bench-top pasteuriser. The canned products were stored at +5°C for 1 week and then assessed by a taste panel. The results are shown in Table 3. 20 TABLE 3 Product (S / C) Taste 1 100 Just acceptable - sweetness builds up in mouth and tea flavour is weak. 2 67 Well-balanced flavour; not bitter and has refreshing tea character. 3 50 Becoming unbalanced; slightly bitter. 4 33 Too bitter; refreshing but not pleasurable. 5 54 Well-balanced flavour; not bitter and has refreshing tea character. CLAIMS A beverage having a total energy content of less than 17 kJ per 100 g, wherein the beverage comprises catechins in amount (C) of from 0.04 to 0.4% by weight of the beverage and non-nutritive sweetener in an amount such that the sweetness (S) of the non-nutritive sweetener in terms of sucrose is less than 7% by weight of the beverage, wherein S is defined by the following equation: 10 wherein Ri is the sweetness of non-nutritive sweetener i relative to an equivalent weight of sucrose .and m± is the amount of non-nutritive sweetener i present in percent by weight of the beverage, characterised in that the beverage 15 comprises non-nutritive sweetener in an. amount satisfying the following condition: 34 2. A beverage according to claim 1 wherein the total energy 2 0 content is less than 5 kJ per 100 g, preferably less than 2 kJ per 100 g. 3. A beverage according to any one of the preceding claims wherein the beverage comprises sugars in an amount of less 25 than 0.25% by weight of the beverage. 4. A beverage according to any one of the preceding claims wherein the beverage comprises non-nutritive sweetener in an amount of between 0.00001 to 10% by weight of the beverage. 30 A beverage according to any one of the preceding claims wherein the non-nutritive sweetener comprises intense sweetener. 5 6. A beverage according to claim 5 wherein the intense sweetener is sucralose, aspartame, acesulfame K, or a mixture thereof. 7. A beverage according to any one of the preceding claims wherein the beverage is a tea-based beverage. 8. A beverage according to any one of the preceding claims 10 wherein the beverage has a pH of from 2 to 5. 9. A beverage according to claim 8 wherein the beverage has a pH of from 3 to 4. 15 10. A beverage according to any one of the preceding claims wherein the beverage comprises organic acid. 11. A beverage according to claim 10 wherein the organic acid comprises malic acid and/or a salt thereof. 20 12. A beverage according to -any preceding claim wherein the beverage comprises flavour. 13. A beverage 'according to claim 12 wherein the beverage 25 comprises the flavour.in an amount of between 0.001 and 0.5% by weight of the beverage. 14. A beverage according to claim 12 or claim 13 wherein the flavour comprises fruit flavour. 30 15. A beverage according to claim 14 wherein the fruit is pineapple. AMENDED SHEET 16. A beverage aceox'di-ng to any one of the preceding claims wherein the beverage comprises caffeine in an amount of between 0.001 to 0.1% by weight of the beverage. 5 17. A method of controlling the bodyweight and/or shape of an individual comprising the step of administering to the individual a beverage according to any one of the preceding claims. 10 18. Use of a beverage according to any one of claims 1 to 16.for the management of the bodyweight ai'id/or shape of an individual.

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