Title of Invention	NOVEL CRYSTALLINE POLYMORPHIC FORM OF A CAMPTOTHECIN ANALOUGE
Abstract	Disclosed herein is a novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86, 27.18, 27.44, 28.10, 28.76, 29.42, 29.68 and 30.02. The invention also discloses processes for preparation of Topotecan hydrochloride form A.

Title of Invention

NOVEL CRYSTALLINE POLYMORPHIC FORM OF A CAMPTOTHECIN ANALOUGE

Abstract

Disclosed herein is a novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86, 27.18, 27.44, 28.10, 28.76, 29.42, 29.68 and 30.02. The invention also discloses processes for preparation of Topotecan hydrochloride form A.

Full Text	FORM 2 THE PATENT ACT 1970 (39 of 1970) & The Patents Rules, 2003 COMPLETE SPECIFICATION (See section 10 and rule!3) 1. TITLE OF THE INVENTION: "Novel Crystalline Polymorphic Form of a Camptothecin Analogue" 2. APPLICANT (a) NAME: CIPLA LTD. (b)NATIONALITY: Indian Company incorporated under the Indian Companies ACT, 1956 (c) ADDRESS: 289, Bellasis Road, Mumbai Central, Mumbai - 400 008, Maharashtra, India. 3. PREAMBLE TO THE DESCRIPTION The following specification particularly describes the invention and the manner in which it is to be performed. Technical field: The present invention relates to a novel crystalline polymorphic form of 10-[(dimethylamino) methyl]- 4-ethyl 4,9dihydroxy-lH-pyrano [3',4':6,7] indolizino [1,2-b] quinoline-3, 14 (4H, 12H) dione hydrochloride (Topotecan Hydrochloride) and the process for the synthesis of the same. Background of the invention: Topotecan hydrochloride is (10-[(dimethyl amino) methyl]-4-ethyl-4,9-dihydroxy-lH-pyrano [3', 4' : 6, 7] indolizino[l, 2-b] quinoline-3, 14 (4H, 12H) dione hydrochloride) a compound of formula (I). CH, H3C—N HO .HCI (I) Topotecan is a semi-synthetic analogue of camptothecin, an agent derived from the Oriental yew tree, Camptothecan accuminata. The cytotoxic effects of the camptothecins are believed to be related to their activity as inhibitors of topoisomerase - I, an enzyme involved in the replication and repair of nuclear DNA. As DNA is replicated in dividing cells, topoisomerase-I acts by binding to super-coiled DNA and causing single-stranded breaks in that DNA. As a result, topoisomerase -I is able to relieve the torsional stresses that are introduced into DNA ahead of the replication complex or moving replication fork. Topotecan inhibits topoisomerase-I by stabilizing the covalent complex of enzyme and strand-cleaved DNA, which is an intermediate of the catalytic mechanism, thereby inducing breaks in the protein-associated DNA single-strands, resulting in cell death. 2 Topotecan hydrochloride stops the growth of cancer cells by preventing the development of elements necessary for cell division. US 5,004,758 discloses water soluble Camptothecin analogs, which includes Topotecan (9-dimethylamino methyl-10- hydroxy camptothecin), preferably (S) -Topotecan and its Hydrochloride salt. US 5,734,056 disclose Camptothecin analogs (which include Topotecan) and a process for the preparation of such analogs and its intermediates. US 5,155,225 describes processes for making Pyrano [3',4':6,7]indolizino-[l,2-B] quinolinones. WO2005046608 discloses a novel crystalline form of Topotecan monohydrochloride pentahydrate, corresponding pharmaceutical compositions, methods of preparation and use for anti-viral and other cancer - related diseases. The present invention relates to the solid state physical properties of topotecan hydrochloride. These properties can be influenced by controlling the conditions under which topotecan hydrochloride is obtained in solid form. Solid state physical properties include, for example, the flowability of the milled solid. Flowability affects the ease with which the material is handled during processing into a pharmaceutical product. Another important solid state property of a pharmaceutical compound is its rate of dissolution in aqueous fluid. The rate of dissolution of an active ingredient in a patient's body fluid can have therapeutic consequences since it imposes an upper limit on the rate at which an orally-administered active ingredient can reach the patient's bloodstream. Topotecan Hydrochloride exhibits polymorphism. Polymorphism is the property of some molecules to adopt more than one crystalline form in the solid state. A single molecule can give rise to a variety of crystalline solids having distinct physical properties that can 3 be measured in a laboratory like its thermal behaviour, e.g. melting point and differential scanning calorimetry (DSC) thermogram, dissolution rate, flowability, X-ray diffraction pattern, infrared absorption spectrum and NMR spectrum. The differences in the physical properties of polymorphs result from the conformation, orientation and intermolecular interactions of adjacent molecules in the crystalline solid. Rate of dissolution of a pharmaceutical compound depends upon its stable crystalline form. The rate of dissolution can have increased effect on the therapeutic efficacy of the administered drug. Hence, this property of the pharmaceutical compound is considered as an important feature in formulating syrups, elixirs and other liquid medicaments. The solid state form of a compound may also affect its behavior on compaction and its storage stability. Thus, it is clear from the foregoing discussion, it would be desirable to have active pharmaceutical ingredient in a stable crystalline form having improved bulk handling and dissolution properties and this becomes the object of the present invention Object of the Invention: The object of the present invention is to provide a novel crystalline polymorphic form of Topotecan hydrochloride hereinafter referred to as Form-A. Another object of the present invention is to provide a process for preparing the novel crystalline polymorphic Form-A of Topotecan hydrochloride from any crystalline form of Topotecan Hydrochloride, pentahydrate or amorphous. Yet another object of the present invention is to provide a process for the preparation of novel crystalline polymorphic Form-A of Topotecan hydrochloride from Topotecan base. Summary of the invention: The present invention discloses a novel polymorphic form of topotecan hydrochloride which is hereinafter designated as form A. In one aspect the present invention provides a 4 novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern (Figurel) having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86,27.18, 27.44,28.10,28.76,29.42, 29.68 and 30.02. In another aspect, there is provided a process of converting Topotecan Hydrochloride of any crystalline form, anhydrous, pentahydrate, or amorphous form into the novel crystalline Topotecan hydrochloride Form A. In a further aspect there is provided a process for preparation of topotecan hydrochloride form A from topotecan base by dissolving the latter in suitable organic solvent. The compound of the present invention, topotecan hydrochloride form A can be formulated into a variety of compositions for administration to humans and mammals. Dosage forms include solid dosage forms like tablets, powders, capsules, sachets, troches and lozenges as well as liquid syrups, suspensions and elixirs. The active ingredient (s) and excipients can be formulated into compositions and dosage forms according to methods known in the art. Brief Description of the Drawing The invention is illustrated by reference to the accompanying drawings described below Figure 1 shows powder X-ray diffractogram of the Form 'A' crystalline polymorph of Topotecan Hydrochloride. Figure 2 shows FT-IR (KBr) spectrum of the Form 'A' crystalline polymorph of Topotecan Hydrochloride. 5 Detailed Description of the Invention The present invention relates to a novel polymorphic form of topotecan hydrochloride which is hereinafter designated as form A. In one aspect the present invention provides a novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern (Figurel) having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86,27.18, 27.44,28.10,28.76,29.42, 29.68 and 30.02. The present invention also provides a novel crystalline polymorphic Form A of Topotecan hydrochloride characterized by FT-IR (KBr) spectrum as depicted in figure 2. Form A provides an infrared spectrum containing peaks at 1743, 1656, 1596, 1560 and 1507. In another aspect the present invention provides a process of preparing a novel crystalline polymorphic Form-A of Topotecan hydrochloride which comprises suspending Topotecan base in a suitable solvent preferably methanol and adding aqueous HC1 and stirring at 25-30°C and further chilling it to at a temperature ranging from -10 to 25°C preferably at 10-15°C and filtering the solid, and drying at 25 - 30°C under vacuum to obtain uniform crystals of Topotecan Hydrochloride Form A. In another aspect the present invention provides a process of converting Topotecan Hydrochloride of any crystalline form, anhydrous, pentahydrate, or amorphous form into the novel crystalline Topotecan hydrochloride Form A which comprises suspending Topotecan hydrochloride in a suitable solvent preferably methanol and stirring at a temperature ranging from -10 to 25°C preferably at 10-15°C, filtering the solid, and drying at 25 - 30°C under vacuum followed by drying at 30 - 35° C for 36 hours to obtain uniform crystals of Topotecan Hydrochloride Form A. 6 The Topotecan hydrochloride Form A of this invention has a water content in the range of 10 to 12%. The novel crystalline polymorphic form A of Topotecan hydrochloride is readily isolated, and displays uniformity, reproducibility, ease and safety of handling in manufacture and stability on isolation and drying. The nature of the invention, its object and advantages are explained hereunder in greater detail in relation to non-limiting exemplary embodiments. Example 1 Preparation of Topotecan Hydrochloride (Form A) 10 grams of Topotecan hydrochloride amorphous form was suspended in 100 ml of Methanol at 25-30°C. The mixture was stirred for 1 hour at 25-30°C and further chilled to 10 - 15° C and stirred for additional 1 hour at 10 - 15° C and filtered; the solid was washed with 5 ml of methanol. The resulting product was dried under vacuum at 25 - 30° C for 5 hours, followed by drying at 30 - 35° C for 36 hours to give 9.0 g of Topotecan hydrochloride Form 'A'. Example 2 Preparation of Topotecan Hydrochloride (Form A) 10.0 g of Topotecan hydrochloride pentahydrate was suspended in 100 ml of methanol and stirred at 25-30°C for 1 hour and further chilled to 10 -15° C and stirred for 1 hour at 10 - 15° C. The resulting solid was filtered and washed with 5 ml of methanol. The solid was dried in vacuum at 25 - 30° C for 5 hours, followed by drying at 30 - 35° C for 36 hours to get 6.0 g of Form 'A'. 7 Example 3 Preparation of Topotecan Hydrochloride (Form A) 10 grams of Topotecan base was suspended in 100 ml Methanol, and 2.4 ml HCl was added at 25-30°C and stirred for 1 hour at 25-30°C, the suspension was further chilled to 10 -15° C and stirred for 1 hour at 10 -15° C and filtered, washed with 5 ml of methanol. The product was dried in vacuum at 25 - 30° C for 5 hours, followed by drying at 30 -35° C for 36 hours to get 8.0 g of Topotecan Hydrochloride Form 'A'. 8 We claim, 1. A novel crystalline polymorphic form of Topotecan hydrochloride Form A characterized by an x-ray powder diffraction pattern as shown in Figure 1 comprising 2 theta angle values at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86, 27.18, 27.44, 28.10, 28.76,29.42, 29.68 and 30.02. 2. A novel crystalline polymorphic form of Topotecan hydrochloride Form A according to claim 1, which provides an infrared spectrum comprising peaks at 1743, 1656,1596,1560 and 1507. 3. A novel crystalline polymorphic form of Topotecan hydrochloride Form A according to Claim 1, wherein the Topotecan hydrochloride has a water content in the range of about 10 to 12%. 4. A process for preparing the novel crystalline polymorphic form of Topotecan. hydrochloride Form A, comprises: suspending Topotecan hydrochloride in methanol; stirring for 1 hr at a temperature ranging from 25 to 30°C; chilling the reaction mass to -10 to 25 °C, stirring for 1 hr and filtering the Topotecan hydrochloride Form 'A'. 5. A process for preparing the novel crystalline polymorphic form of Topotecan hydrochloride Form A, comprises suspending Topotecan base in methanol; adding aqueous HC1 and stirring at 25-30°C, chilling the reaction mass to a temperature ranging from -10 to 25 °C and filtering the Topotecan hydrochloride Form 'A'. 9 6. A novel crystalline polymorphic form of Topotecan hydrochloride Form A and the processes for preparation of Form A as substantially described herein with reference to the foregoing examples 1 to 3. Dated this 10th day of October 2005 Dr. Gopakumar G. Nair Agent for the Applicant 10 ABSTRACT: Disclosed herein is a novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86, 27.18, 27.44, 28.10, 28.76, 29.42, 29.68 and 30.02. The invention also discloses processes for preparation of Topotecan hydrochloride form A. 11

Full Text

FORM 2
THE PATENT ACT 1970
(39 of 1970)
&
The Patents Rules, 2003
COMPLETE SPECIFICATION
(See section 10 and rule!3)

1. TITLE OF THE INVENTION:
"Novel Crystalline Polymorphic Form of a Camptothecin Analogue"
2. APPLICANT
(a) NAME: CIPLA LTD.
(b)NATIONALITY: Indian Company incorporated under the Indian Companies ACT, 1956
(c) ADDRESS: 289, Bellasis Road, Mumbai Central, Mumbai - 400 008, Maharashtra, India.
3. PREAMBLE TO THE DESCRIPTION
The following specification particularly describes the invention and the manner in which it is to be performed.

Technical field:
The present invention relates to a novel crystalline polymorphic form of 10-[(dimethylamino) methyl]- 4-ethyl 4,9dihydroxy-lH-pyrano [3',4':6,7] indolizino [1,2-b] quinoline-3, 14 (4H, 12H) dione hydrochloride (Topotecan Hydrochloride) and the process for the synthesis of the same.
Background of the invention:
Topotecan hydrochloride is (10-[(dimethyl amino) methyl]-4-ethyl-4,9-dihydroxy-lH-pyrano [3', 4' : 6, 7] indolizino[l, 2-b] quinoline-3, 14 (4H, 12H) dione hydrochloride) a compound of formula (I).
CH,

H3C—N
HO

.HCI

(I)

Topotecan is a semi-synthetic analogue of camptothecin, an agent derived from the Oriental yew tree, Camptothecan accuminata. The cytotoxic effects of the camptothecins are believed to be related to their activity as inhibitors of topoisomerase - I, an enzyme involved in the replication and repair of nuclear DNA. As DNA is replicated in dividing cells, topoisomerase-I acts by binding to super-coiled DNA and causing single-stranded breaks in that DNA. As a result, topoisomerase -I is able to relieve the torsional stresses that are introduced into DNA ahead of the replication complex or moving replication fork. Topotecan inhibits topoisomerase-I by stabilizing the covalent complex of enzyme and strand-cleaved DNA, which is an intermediate of the catalytic mechanism, thereby inducing breaks in the protein-associated DNA single-strands, resulting in cell death.
2

Topotecan hydrochloride stops the growth of cancer cells by preventing the development of elements necessary for cell division.
US 5,004,758 discloses water soluble Camptothecin analogs, which includes Topotecan (9-dimethylamino methyl-10- hydroxy camptothecin), preferably (S) -Topotecan and its Hydrochloride salt.
US 5,734,056 disclose Camptothecin analogs (which include Topotecan) and a process for the preparation of such analogs and its intermediates.
US 5,155,225 describes processes for making Pyrano [3',4':6,7]indolizino-[l,2-B] quinolinones.
WO2005046608 discloses a novel crystalline form of Topotecan monohydrochloride pentahydrate, corresponding pharmaceutical compositions, methods of preparation and use for anti-viral and other cancer - related diseases.
The present invention relates to the solid state physical properties of topotecan hydrochloride. These properties can be influenced by controlling the conditions under which topotecan hydrochloride is obtained in solid form. Solid state physical properties include, for example, the flowability of the milled solid. Flowability affects the ease with which the material is handled during processing into a pharmaceutical product.
Another important solid state property of a pharmaceutical compound is its rate of dissolution in aqueous fluid. The rate of dissolution of an active ingredient in a patient's body fluid can have therapeutic consequences since it imposes an upper limit on the rate at which an orally-administered active ingredient can reach the patient's bloodstream.
Topotecan Hydrochloride exhibits polymorphism. Polymorphism is the property of some molecules to adopt more than one crystalline form in the solid state. A single molecule can give rise to a variety of crystalline solids having distinct physical properties that can
3

be measured in a laboratory like its thermal behaviour, e.g. melting point and differential scanning calorimetry (DSC) thermogram, dissolution rate, flowability, X-ray diffraction pattern, infrared absorption spectrum and NMR spectrum.
The differences in the physical properties of polymorphs result from the conformation, orientation and intermolecular interactions of adjacent molecules in the crystalline solid. Rate of dissolution of a pharmaceutical compound depends upon its stable crystalline form. The rate of dissolution can have increased effect on the therapeutic efficacy of the administered drug. Hence, this property of the pharmaceutical compound is considered as an important feature in formulating syrups, elixirs and other liquid medicaments. The solid state form of a compound may also affect its behavior on compaction and its storage stability.
Thus, it is clear from the foregoing discussion, it would be desirable to have active pharmaceutical ingredient in a stable crystalline form having improved bulk handling and dissolution properties and this becomes the object of the present invention
Object of the Invention:
The object of the present invention is to provide a novel crystalline polymorphic form of Topotecan hydrochloride hereinafter referred to as Form-A.
Another object of the present invention is to provide a process for preparing the novel crystalline polymorphic Form-A of Topotecan hydrochloride from any crystalline form of Topotecan Hydrochloride, pentahydrate or amorphous.
Yet another object of the present invention is to provide a process for the preparation of novel crystalline polymorphic Form-A of Topotecan hydrochloride from Topotecan base.
Summary of the invention:
The present invention discloses a novel polymorphic form of topotecan hydrochloride which is hereinafter designated as form A. In one aspect the present invention provides a
4

novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern (Figurel) having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86,27.18, 27.44,28.10,28.76,29.42, 29.68 and 30.02.
In another aspect, there is provided a process of converting Topotecan Hydrochloride of any crystalline form, anhydrous, pentahydrate, or amorphous form into the novel crystalline Topotecan hydrochloride Form A.
In a further aspect there is provided a process for preparation of topotecan hydrochloride form A from topotecan base by dissolving the latter in suitable organic solvent.
The compound of the present invention, topotecan hydrochloride form A can be formulated into a variety of compositions for administration to humans and mammals. Dosage forms include solid dosage forms like tablets, powders, capsules, sachets, troches and lozenges as well as liquid syrups, suspensions and elixirs. The active ingredient (s) and excipients can be formulated into compositions and dosage forms according to methods known in the art.
Brief Description of the Drawing
The invention is illustrated by reference to the accompanying drawings described below Figure 1 shows powder X-ray diffractogram of the Form 'A' crystalline polymorph of Topotecan Hydrochloride.
Figure 2 shows FT-IR (KBr) spectrum of the Form 'A' crystalline polymorph of Topotecan Hydrochloride.
5

Detailed Description of the Invention
The present invention relates to a novel polymorphic form of topotecan hydrochloride which is hereinafter designated as form A. In one aspect the present invention provides a novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern (Figurel) having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86,27.18, 27.44,28.10,28.76,29.42, 29.68 and 30.02.
The present invention also provides a novel crystalline polymorphic Form A of Topotecan hydrochloride characterized by FT-IR (KBr) spectrum as depicted in figure 2. Form A provides an infrared spectrum containing peaks at 1743, 1656, 1596, 1560 and 1507.
In another aspect the present invention provides a process of preparing a novel crystalline polymorphic Form-A of Topotecan hydrochloride which comprises suspending Topotecan base in a suitable solvent preferably methanol and adding aqueous HC1 and stirring at 25-30°C and further chilling it to at a temperature ranging from -10 to 25°C preferably at 10-15°C and filtering the solid, and drying at 25 - 30°C under vacuum to obtain uniform crystals of Topotecan Hydrochloride Form A.
In another aspect the present invention provides a process of converting Topotecan Hydrochloride of any crystalline form, anhydrous, pentahydrate, or amorphous form into the novel crystalline Topotecan hydrochloride Form A which comprises suspending Topotecan hydrochloride in a suitable solvent preferably methanol and stirring at a temperature ranging from -10 to 25°C preferably at 10-15°C, filtering the solid, and drying at 25 - 30°C under vacuum followed by drying at 30 - 35° C for 36 hours to obtain uniform crystals of Topotecan Hydrochloride Form A.
6

The Topotecan hydrochloride Form A of this invention has a water content in the range of 10 to 12%.
The novel crystalline polymorphic form A of Topotecan hydrochloride is readily isolated, and displays uniformity, reproducibility, ease and safety of handling in manufacture and stability on isolation and drying.
The nature of the invention, its object and advantages are explained hereunder in greater detail in relation to non-limiting exemplary embodiments.
Example 1
Preparation of Topotecan Hydrochloride (Form A)
10 grams of Topotecan hydrochloride amorphous form was suspended in 100 ml of Methanol at 25-30°C. The mixture was stirred for 1 hour at 25-30°C and further chilled to 10 - 15° C and stirred for additional 1 hour at 10 - 15° C and filtered; the solid was washed with 5 ml of methanol. The resulting product was dried under vacuum at 25 - 30° C for 5 hours, followed by drying at 30 - 35° C for 36 hours to give 9.0 g of Topotecan hydrochloride Form 'A'.
Example 2
Preparation of Topotecan Hydrochloride (Form A)
10.0 g of Topotecan hydrochloride pentahydrate was suspended in 100 ml of methanol and stirred at 25-30°C for 1 hour and further chilled to 10 -15° C and stirred for 1 hour at 10 - 15° C. The resulting solid was filtered and washed with 5 ml of methanol. The solid was dried in vacuum at 25 - 30° C for 5 hours, followed by drying at 30 - 35° C for 36 hours to get 6.0 g of Form 'A'.
7

Example 3
Preparation of Topotecan Hydrochloride (Form A)
10 grams of Topotecan base was suspended in 100 ml Methanol, and 2.4 ml HCl was added at 25-30°C and stirred for 1 hour at 25-30°C, the suspension was further chilled to 10 -15° C and stirred for 1 hour at 10 -15° C and filtered, washed with 5 ml of methanol. The product was dried in vacuum at 25 - 30° C for 5 hours, followed by drying at 30 -35° C for 36 hours to get 8.0 g of Topotecan Hydrochloride Form 'A'.
8

We claim,
1. A novel crystalline polymorphic form of Topotecan hydrochloride Form A characterized by an x-ray powder diffraction pattern as shown in Figure 1 comprising 2 theta angle values at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86, 27.18, 27.44, 28.10, 28.76,29.42, 29.68 and 30.02.
2. A novel crystalline polymorphic form of Topotecan hydrochloride Form A according to claim 1, which provides an infrared spectrum comprising peaks at 1743, 1656,1596,1560 and 1507.
3. A novel crystalline polymorphic form of Topotecan hydrochloride Form A according to Claim 1, wherein the Topotecan hydrochloride has a water content in the range of about 10 to 12%.
4. A process for preparing the novel crystalline polymorphic form of Topotecan. hydrochloride Form A, comprises: suspending Topotecan hydrochloride in methanol; stirring for 1 hr at a temperature ranging from 25 to 30°C; chilling the reaction mass to -10 to 25 °C, stirring for 1 hr and filtering the Topotecan hydrochloride Form 'A'.
5. A process for preparing the novel crystalline polymorphic form of Topotecan hydrochloride Form A, comprises suspending Topotecan base in methanol; adding aqueous HC1 and stirring at 25-30°C, chilling the reaction mass to a temperature ranging from -10 to 25 °C and filtering the Topotecan hydrochloride Form 'A'.
9

6. A novel crystalline polymorphic form of Topotecan hydrochloride Form A and the processes for preparation of Form A as substantially described herein with reference to the foregoing examples 1 to 3.
Dated this 10th day of October 2005
Dr. Gopakumar G. Nair Agent for the Applicant
10

ABSTRACT:
Disclosed herein is a novel crystalline polymorphic Form-A of Topotecan hydrochloride which is characterized by an XRD pattern having peak positions at 6.08, 6.94, 8.10, 9.96, 10.16, 11.68, 12.28, 13.08, 13.62, 14.32, 15.44, 16.46, 16.56, 17.58, 18.42, 19.32; 20.14, 21.22, 21.88, 22.54, 22.72, 23.38, 24.14, 24.36, 24.78, 25.02, 25.50, 26.42, 26.86, 27.18, 27.44, 28.10, 28.76, 29.42, 29.68 and 30.02. The invention also discloses processes for preparation of Topotecan hydrochloride form A.
11

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1274-MUM-2005-CLAIMS(AMENDED)-(7-5-2012).pdf

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1274-mum-2005-drawings.pdf

1274-MUM-2005-EP DOCUMENT (5-9-2012).pdf

1274-MUM-2005-EP DOCUMENT(5-9-2012).pdf

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1274-MUM-2005-FORM 26(7-5-2012).pdf

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1274-mum-2005-form 3(23-11-2006).pdf

1274-MUM-2005-FORM 3(5-9-2008).pdf

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255388

Indian Patent Application Number

1274/MUM/2005

PG Journal Number

08/2013

Publication Date

22-Feb-2013

Grant Date

18-Feb-2013

Date of Filing

10-Oct-2005

Name of Patentee

CIPLA LIMITED

Applicant Address

289, BEELLASIS ROAD,MUMBAI CENTRAL, MUMBAI 400 008

Inventors:

#	Inventor's Name	Inventor's Address
1	PATHI SRINIVAS LAXMINARAYAN	2475/24, 7th B MAIN R P C LAYOUT, VIJAYNAGAR BANGALORE 560 040 KARNATAKA
2	KANATHALA, SHASHI REKHA	3:1, F-11 SHANTIVAN CHS, SECTOR-7, SANPADA, NEW MUMBAI-400 706
3	GANGRADE, MANISH GOPALDAS	L 7/302, LOK KEDAR SOCIETY J. DOSSA ROAD, MULUND (WEST), MUMBAI - 400 080
4	KANKAN, RAJENDRA NARAYANRAO	A-3/5, N.B.D. SOCIETY, N.S.S. ROAD, GHATKOPAR, MUMBAI-400 084
5	RAO DHARAMARAJ RAMACHANDRA	4/403, GARDEN ENCLAVE, POKHRAN ROAD 2, THANE(W)- 400 601

PCT International Classification Number

A61K31/4745

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA