Title of Invention

4-BENZYL-[1,2,4]THIADIAZOLIDINE-3,5-DIONE DERIVATIVES AS GSK-3 INHIBITORS

Abstract Provided are thiadiazolidine compounds of formula I wherein R is an organic group having at least 8 atoms selected from C or O, which is not linked directly to the N through a -C(O)- and comprising at least an aromatic ring, and their pharmaceutical compositions. These compounds are selective GSK-3 inhibitors and have improved bioavailability. They are useful for the treatment of GSK-3 mediated diseases, among others Alzheimer's disease, type 11 diabetes, depression and brain injury.
Full Text GSK-3 INHIBITORS
FIELD OF THE INVENTION
The present invention relates to enzyme inhibitors, and more particularly to heterocyclic inhibitors of glycogen synthase kinase 3P, GSK-3, to processes of preparation of such compounds, to pharmaceutical compositions comprising them, and to their use for the treatment and or prophylaxis of a disease in which GSK-3 is involved, such as Alzheimer's disease or non-insulin dependent diabetes mellitus.
BACKGROUND OF THE INVENTION
The search for new therapeutic agents has been greatly aided in recent years by better understanding of the structure of enzymes and other biomolecules associated with target diseases. One important class of enzymes that has been the subject of extensive study is the protein kinases. Many diseases are associated with abnormal cellular responses triggered by protein kinase-mediated events. These diseases include autoimmune diseases, inflammatory diseases, neurological and neurodegenerative diseases, cancer, cardiovascular diseases, allergies and asthma, Alzheimer's disease or hormone-related diseases. Accordingly, there has been a substantial effort in medicinal chemistry to find protein kinase inhibitors that are effective as therapeutic agents.
Glycogen synthase kinase-3 (GSK-3) is a serine/threonine protein kinase comprised of a and (3 isoforms that are each encoded by distinct genes (Coghlan et al., Chemistiy & Biology?, 7. 793-803 (2000); Kim and Kimmel, Curr. Opinion Genetics Dev., 10, 508-514 (2000)). The threonine/serine kinase glycogen synthase kinase-3 (GSK-3) fulfills a pivotal role in various receptor-linked signalling pathways (Doble, BW, Woodgett, JR J.Cell Sci. 2003, 116:1175-1186). Dysregulation within these pathways is considered a crucial event in the development of several prevalent human disorders, such as type II diabetes (Kaidanovich O, Eldar-Finkelman H. Expert Opm. Ther. Targets, 2002. 6:555-561), Alzheimer's disease (Grimes CA, Jope RS. Prog.Newobioi 2001, 65:391-426) , CNS disorders such as manic depressive disorder and neurodegenerative diseases, and chronic inflammatory disorders (Hoeflich KP. Luo

J, Rubie EA, Tsao MS, Jin O, Woodgett J, Nature 2000, 406:86-90). These diseases may be caused by, or result in, the abnormal operation of certain cell signalling pathways in which GSK-3 plays a role.
GSK-3 has been found to phosphorylate and modulate the activity of a number of regulatory proteins. These proteins include glycogen synthase which is the rate limiting enzyme necessary for glycogen synthesis, the microtubule associated protein Tau, the gene transcription factor P-catenin, the translation initiation factor elF2B, as well as ATP citrate lyase, axin, heat shock factor-1, c-Jun, c-Myc, c-Myb, CREB, and CEPBa. These diverse protein targets implicate GSK-3 in many aspects of cellular metabolism, proliferation, differentiation and development.
Currently, inhibition of GSK-3 may represent a viable strategy to develop novel medicinal entities for the treatment of such unmet diseases (Martinez A, Castro A, Dorronsoro 1, Alonso M, Med. Res. Rev., 2002, 22:373-384) through insulin mimicry, tau dephosphorylation and amyloid processing, or transcriptional modulation respectively.
Among the great diversity of chemical structures with GSK-3 inhibition already found (Dorronsoro, I; Castro, A; Martinez, A Exp Opin Titer Patents 2002, 12:1527-1536; Alonso,M. and Martinez, A. Currrent Medicinal Chemistry 2004, 11, 753-761), the 2,4-disubstituted thiadiazolidinone (TDZD) are presented as the first ATP-non competitive GSK-3 inhibitors (Martinez A, Alonso M, Castro A, Perez C, Moreno F, J Med Chem, 2002, 45:1292-1299; WO 01 85685 and US 2003/0195238). These compounds have great interest since they are selective and do not show inhibition on other several kinases such as PKA, PKC, CK-2 and CDKl/cyclin B. However, thiadiazolidinones have the tendency to react with nucleophiles and this property may jeopardize their drug potential.
There is still a need to find good GSK-3 inhibitors, being both effective and selective, and having good "drugability" properties, i.e. good pharmaceutical properties related to administration, distribution, metabolism and excretion.

DESCRIPTION OF THE INVENTION
Taking advantage of some of our molecular modelling results and theories, we have designed and synthesized a second generation of 2,4-disubstituted thiadiazolidinones (TDZD) which are very stable against thiol-containing biological molecules such as glutathion and BSA (bovine serum albumina). Surprisingly, these compounds have also a very favourable drugable profile, in particular oral bioavailability and blood brain barrier penetration.
In one aspect the invention is directed to compounds of general formula 1:

i
wherein:
Ri is an organic group having at least 8 atoms selected from C or O, which is not linked directly to the N through a -C(O)- and comprising at least an aromatic ring; R:1 , Rh, R2, R.4 R4, R5. R6 are independently selected from hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted aryl, substituted or unsubstituted heterocyclyl, -COR7. -C(0)OR7, -C(0)NR7R8 -C=NR7, -CN, -OR7. -OC(0)R?, -S(0),-R7, -NR7R,s, -NR7C(0)Rs, -N02, -N=CR7R8 or halogen, tisO. 1,2 or3,
R7 and Rs are each independently selected from hydrogen, substituted or unsubstituted alkyl. substituted or unsubstituted cycloalkyK substituted or unsubstituted alkenyl. substituted or unsubstituted aryl. substituted or unsubstituted

heterocyclyl, substituted or unsubstituted alkoxy, substituted or unsubstituted aryloxy, halogen;
wherein Ra and Rb together can form a group =0, and wherein any pair Ra Ro. R2 R3, R3 R4, R4 R5, R5 R6, R6 Rb, or R7R8 can form together a cyclic substituent; or a pharmaceutically acceptable salt, prodrug or solvate thereof.
We have found that compounds with a benzyl like structure at position 4 and a bulky group comprising an aromatic ring or rings at position 2 of the thiadiazolidinones interact optimally with the GSK-3 enzyme while at the same time presenting good drugability properties.
Preferred compounds are those in which Ri has an aromatic group having at least 10 aromatic carbons. These compounds show good activity, stability and reduced binding to plasma proteins like glutatione and albumin.
Also preferred are compounds in which R| has an aromatic group directly linked to the N of the thiadiazolidine.
In a particular embodiment compounds in which Rj is a naphthyl group are preferred, most preferably if Ri is a a-naphthyl group.
Another preferred class of compounds are those in which the substituent at position 4 of the TDZD is an unsubstituted benzyl group.
In another aspect the invention is directed to pharmaceutical compositions which comprise a compound according to formula (I) or a pharmaceutically acceptable salt, prodmg or solvate thereof, and a pharmaceutically acceptable earner, adjuvant or vehicle. In a preferred embodiment the formulation is oral.
The present invention is also directed to the use of the above defined compounds in the manufacture of a medicament, preferably for a GSK-3 mediated disease or condition.

Alternatively, the invention is also directed to a method of treatment of a GSK-3 mediated disease or condition comprising the administration of an effective amount of a compound of formula 1 as defined above, or a or a pharmaceutically acceptable salt, prodrug or solvate thereof, to a patient in need thereof.
In another aspect, the invention is directed to the use of the above defined compounds as reactives for biological assays, preferably as a reactive for GSK-3
inhibition.
In another aspect the invention is directed to a process for preparing a compound of formula I above by reaction of the appropriate benzyl isothiocyanate with a isocyanate of formula Ri-N=C=0*
DETAILED DESCRIPTION OF THE INVENTION
The typical compounds of this invention selectively inhibit GSK-3(3 without inhibition of other protein kinases such as PKA, PKC, CK-2 and CdK2, which could eliminate the effects. Additionally they do not bind significantly to model proteins such as Glutathione and Bovine Serum Albumin which is a good indication of their stability in plasma. They also show good absorption and blood brain banner permeability as demonstrated by the examples.
In the above definition of compounds of formula (I) the following terms have the meaning indicated:
"Alkyl" refers to a straight or branched hydrocarbon chain radical consisting of carbon and hydrogen atoms, containing no saturation, having one to eight carbon atoms, and which is attached to the rest of the molecule by a single bond, e. g., methyl, ethyl, n-propyK i-propyl, n-butyl, t-butyl, n-pentyl, etc. Alkyl radicals may be optionally substituted by one or more substituents such as halo, hydroxy, alkoxy, carboxy, cyano. carbonyl, acyl, alkoxycarbonyl, amino, nitro, mercapto and alkylthio.

"Alkoxy" refers to a radical of the formula -ORa where Ra is an alkyl radical as defined above, e. g., methoxy, ethoxy, propoxy, etc.
"Alkoxycarbonyl" refers to a radical of the formula -C(0)ORa where Ra is an alkyl radical as defined above, e. g., methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, etc.
"Alkylthio" refers to a radical of the formula -SRa where Ra is an alkyl radical as defined above, e. g., methylthio, ethylthio, propylthio, etc.
"Amino" refers to a radical of the formula-NH2, -NHRa or -NRaRb, Wherein Ra and Rb are as defined above.
"Aryl" refers to a phenyl, naphthyl, indenyl, fenanthryl or anthracyl radical, preferably phenyl or naphthyl radical. The aryl radical may be optionally substituted by one or more substituents such as hydroxy, mercapto, halo, alkyl. phenyl, alkoxy, haloalkyl, nitro, cyano, dialkylamino, aminoalkyi, acyl and alkoxycarbonyl, as defined herein.
"Aralkyl" refers to an aryl group linked to an alkyl group. Preferred examples include benzyl and phenethyl.
"Acyl" refers to a radical of the formula-C(0)-Rc and -C(0)-Rd where Re is an alkyl radical as defined above and Rd is an aryl radical as defined above, e. g., acetyl, propionyl, benzoyl, and the like.
"Aroylalkyl" refers to an alkyl group substituted with -Ra-C(0)-Rd, wherein Ra is an alkyl radical. Preferred examples include benzoylmethyl.[verificar si es correcto]
"Carboxy" refers to a radical of the formula -C(0)OH.
"Cycloalkyl" refers to a stable 3-to 10-membered monocyclic or bicyclic radical

which is saturated or partially saturated, and which consist solely of carbon and hydrogen atoms. Unless otherwise stated specifically in the specification, the term "cycloalkyl" is meant to include cycloalkyl radicals which are optionally substituted by one or more such as alkyl, halo, hydroxy, amino, cyano, nitro, alkoxy, carboxy and
alkoxycarbonyl.
"Fused aryl" refers to an aryl group, especially a phenyl or heteroaryl group, fused to another ring.
"Halo" refers to bromo, chloro, iodo or fluoro.
"Haloalkyl" refers to an alkyl radical, as defined above, that is substituted by one or more halo radicals, as defined above, e. g., trifluoromethyl, trichloromethyl, 2,2,2-trifluoroethyI,l"fluoromethyl-2-fluoroethyl, and the like.
"Heterocycle" refers to a heterocyclyl radical. The heterocycle refers to a stable 3-to 15 membered ring which consists of carbon atoms and from one to five heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur, preferably a 4-to 8-membered ring with one or more heteroatoms, more preferably a 5-or 6-membered ring with one or more heteroatoms. For the purposes of this invention, the heterocycle may be a monocyclic, bicyclic or tricyclic ring system, which may include fused ring systems; and the nitrogen, carbon or sulfur atoms in the heterocyclyl radical may be optionally oxidised; the nitrogen atom may be optionally quatemized ; and the heterocyclyl radical may be partially or fully saturated or aromatic. Examples of such heterocycles include, but are not limited to, azepines, benzimidazole, benzothiazole, furan, isothiazole, imidazole, indole, piperidine, piperazine, purine, quinoline, thiadiazole, tetrahydrofuran.
References herein to substituted groups in the compounds of the present invention refer to the specified moiety that may be substituted at one or more available positions by one or more suitable groups, e. g., halogen such as fluoro. chloro. bromo and iodo ; cyano ; hydroxy! ; nitro : azido ; alkanoyl such as a CI-6 alkanoyl group such as acyl and the like ; carboxamido; alkyl groups including those groups having 1 to

about 12 carbon atoms or from 1 to about 6 carbon atoms and more preferably 1-3 carbon atoms ; alkenyl and alkynyl groups including groups having one or more unsaturated linkages and from 2 to about 12 carbon or from 2 to about 6 carbon atoms ; alkoxy groups having one or more oxygen linkages and from 1 to about 12 carbon atoms or 1 to about 6 carbon atoms ; aryloxy such as phenoxy ; alkylthio groups including those moieties having one or more thioether linkages and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; alkylsulfmyl groups including those moieties having one or more sulfinyl linkages and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; alkylsulfonyl groups including those moieties having one or more sulfonyl linkages and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; aminoalkyl groups such as groups having one or more N atoms and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; carbocylic aryl having 6 or more carbons, particularly phenyl or naphthyl and aralkyl such as benzyl. Unless otherwise indicated, an optionally substituted group may have a substituent at each substitutable position of the group, and each substitution is independent of the other.
Unless otherwise stated, the compounds of the invention are also meant to include compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures except for the replacement of a hydrogen by a deuterium or tritium, or the replacement of a carbon by a 3C- or 4C-enriched carbon or "N-enriched nitrogen are within the scope of this invention.
The term "pharmaceutically acceptable salts, derivatives, solvates , prodrugs" refers to any pharmaceutically acceptable salt, ester, solvate, or any other compound which, upon administration to the recipient is capable of providing (directly or indirectly) a compound as described herein. However, it will be appreciated that non-pharmaceutically acceptable salts also fall within the scope of the invention since those may be useful in the preparation of pharmaceutically acceptable salts. The preparation of salts, prodrugs and derivatives can be carried out by methods known in the art.
For instance, pharmaceutically acceptable salts of compounds provided herein are synthesized from the parent compound which contains a basic or acidic moiety by

conventional chemical methods. Generally, such salts are, for example, prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent or in a mixture of the two. Generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol or acetonitrile are preferred. Examples of the acid addition salts include mineral acid addition salts such as, for example, hydrochloride, hydrobromide, hydroiodide, sulphate, nitrate, phosphate, and organic acid addition salts such as, for example, acetate, maleate, fumarate, citrate, oxalate, succinate, tartrate, malate, mandelate, methanesulphonate and p-toluenesulphonate. Examples of the alkali addition salts include inorganic salts such as, for example, sodium, potassium, calcium, ammonium, magnesium, aluminium and lithium salts, and organic alkali salts such as, for example, ethylenediamine, ethanolamine, N,N-dialkylenethanolamine, triethanolamine, glucamine and basic aminoacids salts.
Particularly favoured derivatives or prodrugs are those that increase the bioavailability of the compounds of this invention when such compounds are administered to a patient (e.g., by allowing an orally administered compound to be more readily absorbed into the blood) or which enhance delivery of the parent compound to a biological compartment (e.g., the brain or lymphatic system) relative to the parent species.
Any compound that is a prodrug of a compound of formula (I) is within the scope of the invention. The term "prodrug" is used in its broadest sense and encompasses those derivatives that are converted in vivo to the compounds of the invention. Such derivatives would readily occur to those skilled in the art, and include, depending on the functional groups present in the molecule and without limitation, the following derivatives of the present compounds: esters, amino acid esters, phosphate esters, metal salts sulfonate esters, carbamates, and amides. Examples of well known methods of producing a prodrug of a given acting compound are known to those skilled in the art and can be found e.g. in Krogsgaard-Larsen et al. "Textbook of Drugdesign and Discovery" Taylor & Francis (april 2002).

The compounds of the invention may be in crystalline form either as free compounds or as solvates (e.g. hydrates) and it is intended that both forms are within the scope of the present invention. Methods of solvation are generally known within the art. Suitable solvates are phannaceutically acceptable solvates. In a particular embodiment the solvate is a hydrate.
The compounds of formula (I) or their salts or solvates are preferably in phannaceutically acceptable or substantially pure form. By phannaceutically acceptable form is meant, inter alia, having a phannaceutically acceptable level of purity excluding nonnal pharmaceutical additives such as diluents and carriers, and including no material considered toxic at normal dosage levels. Purity levels for the drug substance are preferably above 50%, more preferably above 70%o, most preferably above 90%. In a prefen-ed embodiment it is above 95% of the compound of fonnula (I), or of its salts, solvates or prodrugs.
The compounds of the present invention represented by the above described fonnula (I) may include enantiomers depending on the presence of chiral centres or isomers depending on the presence of multiple bonds (e.g. Z, E). The single isomers, enantiomers or diastereoisomers and mixtures thereof fall within the scope of the present invention.
We have found that the compounds of fonnula I above are selective GSK-3 inhibitors (they do not show inhibition on other kinases) and additionally they present good phannacological properties which makes them suitable for drug development. Indeed, through the adequate selection of the size and chemical characteristics of the substituents on the TDZD ring we have obtained compounds that are very stable against plasma molecules such as glutathion and BSA, and have shown good oral bioavailability and blood banner penetration.
Ri comprises an aromatic group, this improves the stability properties. In an embodiment R| has at least 10 aromatic carbons. Alternatively, good compounds are obtained with electron donating groups on the aromatic ring such as alkoxyl or methylendioxy.

Although R1 can be linked to the TDZD through any group as long as it is not -C(O)- (because of degradation and poor stability in plasma), it is preferred that the aromatic group is directly linked to the N of the TDZD.
Representative substituents that can be used as Rj are the following:

Very good results of stability and bioavailability in vivo have been obtained with a bulky aromatic group such as naphthyl. In particular alpha-naphthyl has given good results. When Rj is alpha-naphthyl, it is preferred that it is an unsubstituted alpha-naphthyl.
Concerning the substituent at position 4 of the TDZD, it is preferred that Ra and Rb are H.
In another embodiment it is preferred that R2. R3 R4 R5, R6 are independently selected from hydrogen, substituted or unsubstituted alky], COR7, -C(0)OR7, -OR7, -NR7Rx- or
halogen.




And their salts, prodrugs and solvates.
The compounds of formula (I) defined above can be obtained by available synthetic procedures. Some examples of these procedures are described in WO 01/85685 and US 2003/0195238 and references therein. The content of these documents is incoiporated herein by reference in its entirety.
Therefore in another aspect the invention provides a process for the preparation of a compound of formula (I) or a salt or solvate thereof as claimed in any of claims 1-11, which comprises reacting a benzyl substituted isothiocyanate of formula II

with a compound of formula RrN=C=0.
For example, the following procedure can be used to produce 4-N-benzyl substituted thiadiazolidinones:


The general experimental procedure of Scheme 1 is described for example in Slomczynska, U.; Barany, G., "Efficient Synthesis of l,2,4-Dithiazolidine-3,5-diones (Dithiasuccinoyl-amines) and observations on formation of l,2,4-Thiadiazolidine-3,5-dione by related Chemistry", J. Heterocyclic Chern., 1984, 27, 241-246.
For example, sulfuryl chloride is added dropwise with stirring, under nitrogen atmosphere, preferably at low temperature, preferably at about 5 °C, to a solution of benzyl isothiocyanate and the isocyanate indicated in each case, in a suitable solvent such as hexane, ether or THF. When the addition is finished, the mixture is left to react, for example by stirring for 20 hours at room temperature. After this time, the resulting product is isolated by conventional methods such as suction filtration or solvent evaporation and then, the purification is performed (e.g. by recristallization or silica gel column chromatography using the appropriate eluent).
Other alternative procedures will be apparent to the person skilled in the art, such as the use of any other chlorinating agent instead of sulfuryl chloride, variations in the order of addition of the reactants and reaction conditions (solvents, temperature, etc).
The reaction products may, if desired, be purified by conventional methods, such as crystallisation, chromatography and trituration.
One preferred pharmaceutically acceptable form is the crystalline form, including such form in a pharmaceutical composition. In the case of salts and solvates the additional ionic and solvent moieties must also be non-toxic. The compounds of the

invention may present different polymorphic forms, it is intended that the invention encompasses all such forms.
Another aspect of this invention relates to a method of treating or preventing an GSK-3 mediated disease with an GSK-3 inhibitor as described above, which method comprises administering to a patient in need of such a treatment a therapeutically effective amount of a compound of formula (I) or a pharmaceutical composition thereof.
The terms "GSK-3 mediated disease, or "GSK-3 mediated condition", as used 1 herein, mean any disease or other deleterious condition or state in which GSK-3 is known to play a role. Such diseases or conditions include, without limitation, diabetes, conditions associated with diabetes, chronic neurodegenerative conditions including dementias such as Alzheimer's disease, Parkinson's disease, progressive supranuclear palsy, subacute sclerosing panencephalitic parkinsonism, postencephalitic parkinsonism, pugilistic encephalitis, guam parkinsonism-dementia complex, Pick's disease, corticobasal degeneration, frontotemporal dementia, Huntington's Disease, AIDS associated dementia, amyotrophic lateral sclerosis, multiple sclerosis and neurotraumatic diseases such as acute stroke, epilepsy, mood disorders such as depression, schizophrenia and bipolar disorders, promotion of functional recovery post stroke, cerebral bleeding (for example, due to solitary cerebral amyloid angiopathy), hair loss, obesity, atherosclerotic cardiovascular disease, hypertension, polycystic ovary syndrome, syndrome X. ischaemia, brain injury, especially traumatic brain injury, cancer, leukopenia, Down's syndrome, Lewy body disease, inflammation, chronic inflammatory diseases, cancer and hyperproliferative diseases as hyperplasias and immunodeficiency.
In a particular embodiment of the invention the compounds of formula (I) or their pharmaceutical compositions, e.g. in oral fonn, are used for the treatment of the
Alzheimer's disease.
In another embodiment of the invention the compounds of formula (I) or their pharmaceutical compositions, e.g. in oral form, are used for the treatment of the
diabetes.

In another embodiment of the invention the compounds of formula (I) or their pharmaceutical compositions, e.g. in oral form, are used for the treatment of the depression.
In another embodiment of the invention the compounds of formula (I) or their pharmaceutical compositions, e.g. in oral form, are used for the treatment of brain injury.
The present invention further provides pharmaceutical compositions comprising a compound of this invention, pharmaceutically acceptable salts, derivatives, prodrugs or stereoisomers thereof with a pharmaceutically acceptable carrier, adjuvant, or vehicle, for administration to a patient.
Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules etc.) or liquid (solutions, suspensions or emulsions) composition for
oral, topical or parenteral administration.
In a preferred embodiment the pharmaceutical compositions are in oral form. Suitable dose forms for oral administration may be tablets and capsules and may contain conventional excipients known in the art such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate; disintegrants, for example starch, polyvinylpyrrolidone, sodium starch glycollate or microcrystalline cellulose; or pharmaceutically acceptable wetting agents such as sodium lauryl sulfate.
The solid oral compositions may be prepared by conventional methods of blending, filling or tabletting. Repeated blending operations may be used to distribute the active agent throughout those compositions employing large quantities of fillers. Such operations are conventional in the art. The tablets may for example be prepared by wet or dry granulation and optionally coated according to methods well known in normal pharmaceutical practice, in particular with an enteric coating.

The pharmaceutical compositions may also be adapted for parenteral administration, such as sterile solutions, suspensions or lyophilized products in the appropriate unit dosage form. Adequate excipients can be used, such as bulking agents, buffering agents or surfactants.
The mentioned formulations will be prepared using standard methods such as those described or refen-ed to in the Spanish and US Pharmacopoeias and similar reference texts.
Administration of the compounds or compositions of the present invention may be by any suitable method, such as intravenous infusion, oral preparations, and intraperitoneal and intravenous administration. Oral administration is preferred because of the convenience for the patient and the chronic character of many of the diseases to
be treated.
Generally an effective administered amount of a compound of the invention will depend on the relative efficacy of the compound chosen, the severity of the disorder being treated and the weight of the sufferer. However, active compounds will typically be administered once or more times a day for example 1, 2, 3 or 4 times daily, with typical total daily doses in the range of from 0.1 to 1000 mg/kg/day.
The compounds and compositions of this invention may be used with other drugs to provide a combination therapy. The other drugs may form part of the same composition, or be provided as a separate composition for administration at the same time or at different time.
In another aspect the invention relates to inhibiting GSK-3 activity in a biological sample with the compounds of fonnula (I), which method comprises contacting the biological sample with a GSK-3 inhibitor of fonnula (I). The tenn "biological sample", as used herein, includes, without limitation, cell cultures or extracts thereof; preparations of an enzyme suitable for in vitro assay; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen. tears, or other body fluids or extracts thereof. Thus, in one aspect the invention is

directed to the use of compounds of formula I as reactives for biological assays, in particular as a reactive for GSK-3 inhibition.
The following examples are intended to further illustrate the invention. They should not be inteipreted as a limitation of the scope of the invention as defined in the
claims.
EXAMPLES
Synthesis of compounds
General experimental procedure:
Sulfuryl chloride is added dropwise with stirring, under nitrogen atmosphere, at 5 °C to a solution of benzyl isothiocyanate and the isocyanate indicated in each case, in hexane, ether or THF. When the addition is finished, the mixture is stirred for 20 hours at room temperature. After this time, the resulting product is isolated by suction filtration or by solvent evaporation and then, the purification is performed by reciistallization or silica gel column chromatography using the appropriate eluent. More details can be found in Slomczynska, U.; Barany, G., "Efficient Synthesis of 1,2,4-Dithiazolidine-3,5-diones (Dithiasuccinoyl-amines) and observations on formation of l,2,4-Thiadiazolidine-3,5-dione by related Chemistry", J. Heterocyclic Chem., 1984, 27,241-246.
Example 1
2-Phenethyl-4-benzyl-(l,2,4)thiadiazolidine-3,5-dione (1)
Reagents: Benzyl-isothiocianate (6.5 mmol, 0.85 mL), phenethylisocyanate (6.5 mmol, 0.89 mL) and S02C12 (6.5 mmol, 0.52 mL) in diethyl ether (25 mL). Isolation: solvent evaporation. Purification: silica gel column chromatography (AcOEt/ hexane, 1:4). Yield: 1.5 g (74%), yellow oil.
'H-RMN (CDC13): 2.9 (t, 2H, CH2C//2Ph. 7=7.2 Hz): 3.9 (t. 2H. CHiCHiPh. 7=7.2 Hz); 4.8 (s, 2H, CH2?h)\ 7.2-7.4 (m, 10 H, arom)

,3C-RMN (CDCI3): 34.9 (CH2CH2Ph); 4.9 (CH2CH2Ph); 46.2 (CH2PI1); 126.9; 128.5; 128.6; 136.6 (CaromCH2-PA); 128.1; 128.6; 128.6; 135.0 (C arom CH2CH2PA); 152.6 (3-CO); 165.6 (5-C=0). Anal (C,7H,6N202S), C, H, N, S.
Example 2
4-Benzyl-2-naphthalen-l-yl-[l,2,4]thiadiazolidine-3,5-dione (2)
Reagents: Benzyl-isothiocianate (13 mmol, 1.72 mL), 1-naphthyl-isocyanate (13 mmol, 1.9 mL) and SO2O2 (13 mmol, 1.04 mL) in hexane (50 mL). Isolation: filtration of reaction mixture. Purification: recrystallization from EtOH. Yield: 3.8 g (87%), white needles. mp= 150 °C
'H-RMN (CDCI3): 4.9 (s, 2H, C7/2Ph); 7.3-7.9 (m, 12H, arom.)
13C-RMN (CDCI3): 46.5 (CH2PI1); 128.3; 128.6; 129.0; 135.0 (C arom, Ph)\ 122.0;
125.3; 126.8; 127.2; 127.5; 128.5; 130.8: 134.4 (C arom, naphthyl); 152.2 (3-CO); 165.9 (5-C=0). Anal(Ci9H|4N202S), C, H, N, S.
Example 3 (comparative)
2-(l-adamantyl)-4-benzyl-[l,2.4]thiadiazolidine-3,5-dione (3)
Reagents: Benzylisothiocyanate (6.5 mmol, 0.85 mL), 1-Adamantyl-isocyanate (6.5
mmol, 1.15 g) and SO2CI2 (6.5 mmol, 0.52 mL) in diethyl ether (25 mL). Isolation:
solvent evaporation. Purification: silica gel column chromatography (AcOEt/ hexane,
1:4).
Yield: 0.89 g (40%), yellow crystals. mp= 128.8 °C
'H-RMN (CDC13): 1.7 (m. 6H, adamantyl): 2.2 (m. 3H, adamantyl): 2.3 (m, 6H. adamantyl); 4.8 (s, 2H, CH2Ph): 7.2-7.4 (m. 5H. arom)
l3C-RMN (CDCI3): 29.9; 30.0; 35.9; 41.0; 60.0 (C adamantyl); 45.3 (CH2Ph); 127.8; 128.5; 128.6; 135.4 (C arom).

Anal. (C,9H22N202S), C, H, N, S.
Example 4
4-Benzyl-2-(4-methyl-benzyl)-[L2,4]thiadiazolidine-3,5-dione (4)
Reagents: Benzylisothiocyanate (6.5 mmol, 0.85 mL), 4-methylbenzyl-isocyanate (6.5 mmol, 0.90 mL) and SO2CI2 (6.5 mmol, 0.52 mL) in diethyl ether (25 mL). Isolation: filtration of reaction mixture. Purification: recrystallization from MeOH. Yield: 0.95 g (48%), white solid. mp= 69.1 °C
'H-RMN (CDCI3): 2.4 (s, 3H, CH3); 4.7 (s, 2H, C//2-Ph); 4.8 (2H, s, CH2~Ph)] 12 (s, 4H, arom); 7.2-7.5 (m, 5H, arom).
13C-RMN (CDCI3): 21.3 (CH3); 45.9 (CH2Ph); 48.5 (CH2Ph); 128.1; 128.6; 128.7; 135.0 (Carom); 128.4; 129.5; 131.1; 138.6 (C arom); 152.8 (3-CO); 165.7 (5-C=0). Anal. (Ci7H|6N202S), C, H, N, S.
Example 5
4-Benzyl 2-((3,4-methylendioxy)phenyl) -[K2,4]thiadiazolidine-3,5-dione (5)
Reagents: Benzylisothiocyanate (6.5 mmol, 0.85 mL), 3,4-(methylendioxy)phenyl-isocyanate (6.5 mmol, 1.06 mL) and SO2CI2 (6.5 mmol, 0.52 mL) in diethyl ether (25 mL). Isolation: filtration of reaction mixture. Purification: recrystallization from MeOH. Yield: 1.4 g (66%), white solid. mp=126.5 °C
'H-RMN (CDCh): 4.9 (s, 2H, CH2Ph); 6.0 (s, 2H, 0-C//2-0); 6.7-7.0 (m, 3H, arom);
7.3-7.5 (m, 5H, arom)
,3C-RMN(CDCl3):46.2(CH2Ph); 128.2; 128.6; 129.0; 134.9 (C arom); 101.8 (0-CH2-
O); 106.4; 108.3; 118.2; 129.0; 148.1; 146.8 (C arom); 151.2 (3-C=0); 164.9 (5-C=0) Anal. (C16H12N2O4S), C, H, N, S.
Example 6
4-Benzyl-2-diphenylmethyl-l,2,4-thiadiazolidine-3.5-dione (6)

Reagents: Benzylisothiocyanate (6.5 mmol, 0.85 mL), diphenylmethyl-isocyanate (6.5 mmol, 1.23 mL) and SO2CL (6.5 mmol, 0.52 mL) in diethyl ether (25 mL). Isolation: filtration of reaction mixture. Purification: recrystallization from MeOH. Yield: 1.79 g (80%), white solid. mp=l 11.5 °C
'H-RMN (CDCI3): 4.85 (s, 2H,C//2Ph); 6.8 (s, 1H, Ph-C//-Ph); 7.2-7.4 (m, 15 H, arom) 13C-RMN (CDCI3): 45.9 (CH2Ph); 61.6 (Ph-C//-Ph); 128.0; 128.6; 128.7; 135.0 (C
arom); 128.1; 128.5; 128.5; 137.5 (2xPh); 152.6 (3-C=0); 165.8 (5-C=0) Anal. (C22H,8N202S), C, H, N, S.
Example 7
4-Benzyl-2-(4-metoxybenzyl)-[ 1.2,4]thiadiazolidine-3,5-dione (7)
Reagents: Benzylisothiocyanate (6.5 mmol, 0.85 mL), /?-metoxybenzyl-isocyanate (6.5
mmol, 0.92 mL) and SO2CL (6.5 mmol, 0.52 mL) in diethyl ether (25 mL). Isolation:
solvent evaporation. Purification: silica gel column chromatography (AcOEt/ hexane,
1:4).
Yield: 1.30 g (61%), yellowish solid. mp=86.4 "C
'H-RMN (CDCI3): 3.8 (s, 3H, CH3); 4.7 (s, 2H, C//2-Ph-OMe); 4.8(s, 2H, C//2-Ph);
7.2-7.4 (m, 5H, arom); 6.8 (d, 2H. 7=8.6 Hz); 7.2 (d, 2H, J=8.6 Hz)(Arom)
l3C-RMN (CDCI3): 45.9 (CH2-Ph); 48.2 (CH2-Ph-OMe); 55.2 (O-CH3); 128.0; 129.8;
128.4; 135.0 (C arom-Ph); 126.2; 128.5; 114.2; 159.7 (C arom Ph-OMe); 152.7 (3-
C=0); 165.6 (5-C=0).
Anal. (C,7HI3N203S), C, H, N, S.
Example 8
4-Benzyl-2-(2- tert-butyl-6-methyl-phenyl)-(l,2,4)thiadiazolidine-3,5-dione (8)
Reagents: Benzylisothiocyanate (3.5 mmol, 0.45 mL), 2-ter/-butyl-6-methylisocyanate (3.5 mmol. 662.5 mg) and SO2CI2 (3.5 mmol, 0.25 mL) in diethyl ether (15 mL). Isolation: solvent evaporation. Purification: silica gel column chromatography (AcOEt hexane, 1:10).Yield: 0.17 g (14%), brown solid. mp= 89.8 °C

1 H-RMN (CDCU): 1.4 (s, 9H, C(C//3)3); 2.1 (s, 3H, C//3); 4.9 (2d, 2H, C//2-Ph, J= 6.3
Hz); 7.1-7.5 (m, 8H, arom)
l3C-RMN (CDCI3): 17.8 (CH3); 31.9 (C(CH3)); 35.9 (C(CH3)); 46.2 (CLb-Ph); 126.1;
128.6; 128.5; 135.1 (C arom-Bn); 131.5; 150.4; 139.4; 128.1; 129.5; 129.9 (C arom-Ph);
152.4 (3-C=0); 165.7 (5-C=0)
Anal. (C20H22N2O2S), C, H, N, S.
Example 9
4-Benzyl-2-(2-benzyl-phenyl)-[l,2,4]thiadiazolidine-3,5-dione (9)
Reagents'. Benzylisothiocyanate (6.5 mmol, 0.85 mL), 2-benzylphenyl-isocyanate (6.5 mmol, 0.82 mL) and SO2CI2 (6.5 mmol, 0.5 mL) in diethyl ether (25 mL). Isolation: filtration of reaction mixture. Purification: recrystallization from EtOH Yield: 1.50 g (62%), white solid. mp= 154.9 °C
'H-RMN (CDCI3): 3.9 (s, 2H, Ph-C7/2-Ph); 4.86 (s, 2H, C//2PI1); 6.9-7.5 (m, 14 H,
arom)
l3C-RMN (CDCI3): 38.1 (Ph-CH2-Ph); 46.1(CH2-Ph); 135.1; 128.5; 128.6; 129.2 (C-
Bn); 138.9; 129.9; 131.6; 128.4; 127.9; 133.1 (/^-CLb-Ph); 140.9; 128.7; 128.6; 126.4 (Ph-CH2-P/i); 151.2 (3-C=0); 166.0 (5-C=0) Anal. (C22H18N2O2S), C, H, N, S.
Example 10
4-Benzyl-2-(4-phenoxyphenyl)-[l,2,4]thiadiazolidine-3,5-dione (10)
Reagents: Benzylisothiocyanate (13 mmol, 1.6 mL), 4-phenoxyphenyl-isocyanate (13 mmol, 2.3 mL) and SO2CI2 (13 mmol, 1 mL) in diethyl ether (50 mL). Isolation: filtration of reaction mixture. Purification: recrystallization from EtOH. Yield: 4.12 g (84%), white solid. mp=88.8 °C
1 H-RMN (CDCI3): 4.92 (s, 2H, CH2Ph); 7.0-7.6 (m, 14 H. arom)

'T-RMN (CDCI3): 46.1 (CH2PI1); 134.9; 128.7; 129.1; 128.3 (CH2--PA); 130.1; 125.7;
119.2; 156.3 (P/7-O-Ph); 156.3; 119.1; 129.8; 123.8 (Ph-O-P/?); 151.1 (3-C=0); 165.0
(5-CO)
Anal. (C2]H,6N203S), C, H. N, S.
Biological Methods Example 11
GSK-3P inhibition
The GSK-3P activity was determined by incubation of a mixture of recombinant human GSK-3 enzyme, a phosphate source and GSK-3 substrate in the presence and in the absence of the corresponding test compound, and by measuring the GSK-3 activity of this mixture.
Recombinant human glycogen synthase kinase 3B was assayed in MOPS 8 mM pH 7.3, EDTA 0.2 mM, MgClo 10 mM and sodium orthovanadate 0.25 mM in the presence of 62.5 μM of Phospho-Glycogen Synthase Peptide-2 (GS-2), 0.5 μCi y-33P-ATP and unlabelled ATP at a final concentration of 12.5 μM. The final assay volume was 20 \x\. After incubation for 30 minutes at 30 °C, 15 μL aliquots were spotted onto P81 phosphocellulose papers. Filters were washed four times for at least 10 minutes each and counted with 1.5 ml of scintillation cocktail in a scintillation counter. The compounds IC50 values were calculated analyzing inhibition curves by non-linear regression using GraphPad Prism.
The IC50 (concentration at which 50 % of enzyme inhibition is shown) values are gathered in table 1.
Table 1.- IC50 values



Example 12
Binding to GSH and BSA
Sample Preparation
The compounds (working solution at 1 mM) were incubated during 30 minutes at room
temperature with Glutathione (Sigma) and Bovine serum albumin (Fraction V) (Sigma)
at equimolecular concentrations (1 mM ). After this time the solution was filtrated and
analysed by HPLC-UV/MS.
Chromatographic methods
HPLC was performed with a symmetry CI8 ( 2.1x150mm. 3.5μm column using a
Waters Alliance 2695 with a 2996 photodiode array and ZQ2000 mass spectrometer



unbinding compounds in the two assays. The presence of an aromatic group at position 2 (R|) of the TDZD clearly improves the properties of these compounds. This effect is bigger if there are at least 10 aromatic carbons present in the substituent, or electrondonating substituents such as in compounds 5 and 10. We can also observe that when the aromatic group is directly linked to the N of the TDZD the results are better. Best results were obtained with phenyloxyphenyl and with alpha-naphthyl.
These data are clearly better than those of previous TDZD compounds. Indeed, previously described 2,4,-dibenzyl-l,2,4-thiadiazolidine-3,5-dione with a smaller substituent at position 2 of the thiadiazolidinone gives a value of 17,1 in the gluthatione assay and 57,0 in the albumin assay, much lower than for example present compound 4 which has an additional methyl group, and in the range of comparative example 3 which has no aromatic rings. And the compound with R| = benzoyl decomposes during the assays thus performing much worse than the compounds of formula I.
Example 13
Brain permeation after oral and intravenous administration
This study was conducted at CIDA S.A.L., Sta Perpetua de Mogola (Barcelona)
Spain.
The objective of this study was investigate the pharmacokinetic behaviour of
compound 2 (R( = alpha-naphthyl) and its possible accumulation in the brain tissue
after both oral and intravenous administration.
C57/BL6 mice (15-30g) from Charles River laboratories Spain were used in this
study. All the mice had free access to the dried, pelleted standard mouse diet. Water
was available ad libitum. Animals were fasted for 4 hours before treatment, but with
water ad libitum. They were fed 2 hours after administration.
Compound 2 was formulated in 10.0% PEG400. 10.0% Cremophor in bidistilled water. The route of administration was a single oral administration at 20 g/kg (10 mL/kg) and single intravenous administration at 2 mg'kg (10 mL/kg). An additional

experiment was performed at 200 mg/kg by the oral route only to determine
proportionality of absorption.
Four animals (2 males and 2 females) were used at each extraction time. Blood was
heparinised, and after centrifugation (3000rpm, 10 mins, 5°C), two plasma aliquots
stored at -20°C and -30 °C until analysis (HPLC/MS-MS).
The summary of the experiment results is showed in the table 3.

Compound 2 is quickly absorbed from the Gl tract after oral administration. A half-life of 6 hours was found after an oral administration of 20 mg/kg. Compound 2 presented a bioavailability of 31.87%. Levels of Compound 2 were detected in the brain, both after oral and intravenous administration. This shows that compounds of formula I above have good bioavailability properties and are suitable for development as a drug for the treatment of GSK-3 mediated diseases or conditions.








wherein:
Ri is an organic group having at least 8 atoms selected from C or O, which is not
linked directly to the N through a -C(O)- and comprising at least an aromatic ring;
R;l . Rb, R2:, R3, R4, R5, R6 are independently selected from hydrogen, substituted or
unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or
unsubstituted alkenyl, substituted or unsubstituted aryl, substituted or unsubstituted
heterocyclyl, -COR7, -C(0)OR7, -C(0)NR7Rk -C=NR7, -CN, -OR7, -OC(0)R7, -
S(0)rR7, -NR7RS, -NR7C(0)R8, -N02, -N=CR7R* or halogen,
wherein Ra and Rb together can form a group =0, and wherein any pair Ra R2, R2
R3, R3 R4, R4 R5, Rs6R6 , R6 Rb or R7R8 can form together a cyclic substituent;
t is 0, 1,2 or 3,
R7 and R8 are each independently selected from hydrogen, substituted or
unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or
unsubstituted alkenyl, substituted or unsubstituted aryl, substituted or unsubstituted
heterocyclyl, substituted or unsubstituted alkoxy, substituted or unsubstituted
aryloxy. halogen;
or a pharmaceutically acceptable salt, prodrug or solvate thereof.
2. A compound according to claim 1 wherein R| has at least 10 aromatic carbons.

3. A compound according to claims 1 or 2, wherein the aromatic group is directly
linked to the N of the thiadiazolidine.
4. A compound according to claim 3 wherein R\ is a substituted or unsubstituted
naphthyl group.
5. A compound according to claim 4 wherein R\ is a substituted or unsubstituted
alpha-naphthyl group, preferably an unsubstituted alpha-naphthyl group.
6. A compound according to claim 1 wherein R| is a group selected from:

7. A compound according to any of claims 1-6 wherein both Ra and Rb are H.
8. A compound according to any of claims 1-7 wherein R2- R3, R4, R5, R6 are
independently selected from hydrogen, substituted or unsubstituted alkyl. COR7,
> -C(0)OR7. -OR7, -NR7R8, or halogen, wherein R7 and Rs are as defined in claim



11. A process for the preparation of a compound of formula (I) or a salt or solvate thereof as claimed in any of claims 1-10, which comprises reacting a benzyl substituted isothiocyanate of formula II

with a compound of formula R|-N=C=0.

wherein R( is as defined in claim 1, preferably as defined in claim 6.
13. A pharmaceutical composition which comprises a compound as claimed in any
of claims 1-10 or a pharmaceutically acceptable salt, prodrug or solvate thereof
and a phannaceutically acceptable carrier, adjuvant or vehicle.
14. A pharmaceutical composition according to claim 13 for oral administration.
15. Use of a compound as defined in any of claims 1-10 in the manufacture of a medicament.
16. Use according to claim 15 wherein the medicament is for the treatment of a
GSK-3 mediated disease or condition.

17. Use according to claim 16 wherein the disease or condition is diabetes,
conditions associated with diabetes, chronic neurodegenerative conditions
including dementias such as Alzheimer's disease, Parkinson's disease,
progressive supranuclear palsy, subacute sclerosing panencephalitic
parkinsonism, postencephalitic parkinsonism, pugilistic encephalitis, guam
parkinsonism-dementia complex, Pick's disease, corticobasal degeneration,
frontotemporal dementia, Huntington's Disease, AIDS associated dementia,
amyotrophic lateral sclerosis, multiple sclerosis and n euro traumatic diseases
such as acute stroke, epilepsy, mood disorders such as depression, schizophrenia
and bipolar disorders, promotion of functional recovery post stroke, cerebral
bleeding such as due to solitary cerebral amyloid angiopathy, hair loss, obesity,
atherosclerotic cardiovascular disease, hypertension, polycystic ovary syndrome,
syndrome X, ischaemia, brain injury, traumatic brain injury, cancer, leukopenia,
Down's syndrome, Lewy body disease, inflammation, chronic inflammatory
diseases, cancer and hyperproliferative diseases as hyperplasias and
immunodeficiency.
18. Use according to claim 17 wherein the disease is Alzheimer's disease.
19. Use according to claim 17 wherein the disease is type 11 diabetes.
20. Use according to claim 17 wherein the disease is depression.
21. Use according to claim 17 wherein the disease is brain injury.
22. Use of compounds of formula (I) as defined in any of claims 1-10 as reactives for biological assays, preferably as a reactive for GSK-3 inhibition.

Documents:

3684-CHENP-2006 CLAIMS.pdf

3684-CHENP-2006 CORRESPONDENCE OTHERS 19-10-2011.pdf

3684-CHENP-2006 DESCRIPTION (COMPLETE).pdf

3684-CHENP-2006 FORM-13.pdf

3684-CHENP-2006 AMENDED CLAIMS 16-03-2012.pdf

3684-CHENP-2006 AMENDED PAGES OF SPECIFICATION 16-03-2012.pdf

3684-CHENP-2006 EXAMINATION REPORT REPLY RECEIVED 16-03-2012.pdf

3684-CHENP-2006 FORM-1 16-03-2012.pdf

3684-CHENP-2006 FORM-13 25-11-2008.pdf

3684-CHENP-2006 FORM-3 16-03-2012.pdf

3684-CHENP-2006 OTHER PATENT DOCUMENT 16-03-2012.pdf

3684-CHENP-2006 POWER OF ATTORNEY 16-03-2012.pdf

3684-CHENP-2006 FORM-13 13-11-2009.pdf

3684-chenp-2006-abstract.pdf

3684-chenp-2006-claims.pdf

3684-chenp-2006-correspondnece-others.pdf

3684-chenp-2006-description(complete).pdf

3684-chenp-2006-form 1.pdf

3684-chenp-2006-form 3.pdf

3684-chenp-2006-form 5.pdf

3684-chenp-2006-pct.pdf


Patent Number 253384
Indian Patent Application Number 3684/CHENP/2006
PG Journal Number 29/2012
Publication Date 20-Jul-2012
Grant Date 17-Jul-2012
Date of Filing 05-Oct-2006
Name of Patentee NOSCIRA S A
Applicant Address AVDA. DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
Inventors:
# Inventor's Name Inventor's Address
1 DORRONSORO DIAZ, ISABEL AVENIDA DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
2 PANIZO DEL PLIEGO, GEMA AVENIDA DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
3 MEDINA PADILLA, MIGUEL AVENIDA DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
4 MARTINEZ, GIL, ANA AVDA. DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
5 ALONSO CASCON, MERCEDES AVENIDA DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
6 FUERTES HUERTA, ANA AVENIDA DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
7 PEREZ PUERTO, MARIA, JOSE AVENIDA DE LA INDUSTRIA, 52, TRES CANTOS, 28760 MADRID, SPAIN
PCT International Classification Number C07D 285/08
PCT International Application Number PCT/EP05/03613
PCT International Filing date 2005-04-04
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 04075997.9 2004-04-05 EUROPEAN UNION