| Title of Invention | "A PROCESS FOR PREPARATION OF POLY DL-LACTIDE-CO-GLYCOLIDE NANOPARTICLES HAVING STREPTOMYCIN ENCAPSULATED THEREIN" |
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| Abstract | A process for the preparation of Poly DL-lactide-co-glycolide nanoparticles having streptomycin encapsulated therein comprising the steps of, preparation of a polymer solution in an organic solvent and drug solution in water, wherein the ratio of said drug and polymer is 1:1 w/w, mixing the aqueous solutions of steps (i) with the organic solution in a ratio of 1:5-20 volume by volume followed by sonication for 45-120 sec at 4-15°C to obtain a primary emulsion, adding the above emulsion to aqueous PVA and resonicating for 2-5 minutes at 4-15°C, which is subjected to stirring for 18-30 hr. under cold conditions, stirring the emulsion and centrifuging at 8000-12000 rpm for 15-30 minutes at 4-20°C to obtain pellet, washing the said pellets 3-4 times with DW/NS/PBS, reconstituting the same and lyophilizing. |
| Full Text | Another object of this invention is to propose a process for preparing poly DL-lactide-co-glycolide nanoparticles having streptomycin encapsulated therein. DETAILED DESCRIPTION OF THE INVENTION The present invention provides a process for a process for the preparation of Poly DL-lactide-co-glycolide nanoparticles having streptomycin encapsulated therein comprising the steps of: i) preparation of a polymer solution in an organic solvent and drug solution in water, wherein the ratio of said drug and polymer is 1:1 w/w, ii) mixing the aqueous solutions of steps (i) with the organic solution in a ratio of 1:5-20 volume by volume followed by sonication for 45-120 sec at 4-15°C to obtain a primary emulsion, iii) adding the above emulsion to aqueous PVA and resonicating for 2-5 minutes at 4-15°C, which is subjected to stirring for 18-30 hr. under cold conditions, iv) stirring the emulsion and centrifuging at 8000-12000 rpm for 15-30 minutes at 4-20°C to obtain pellet, v) washing the said pellets 3-4 times with DW/NS/PBS, reconstituting the same and lyophilizing. WE CLAIM: 1. A process for the preparation of Poly DL-lactide-co-glycolide nanoparticles having streptomycin encapsulated therein comprising the steps of: i) preparation of a polymer solution in an organic solvent and solution of Streptomycin in water, characterized by the ratio of said Streptomycin and polymer is 1:1 w/w, ii) mixing the aqueous solutions of steps (i) with the organic solution in a ratio of 1:5-20 volume by volume followed by sonication for 45-120 sec at 4-15°C to obtain a primary emulsion, iii) adding the above emulsion to aqueous PVA and resonicating for 2-5 minutes at 4-15°C, which is subjected to stirring for 18-30 hr. under cold conditions, iv) stirring the emulsion and centrifuging at 8000-12000 rpm for 15-30 minutes at 4-20°C to obtain pellet, v) washing the said pellets 3-4 times with DW/NS/PBS, reconstituting the same and lyophilizing. 2. A process as claimed in claim 1, wherein the solution of polymer such as PLG is prepared in an organic solvent preferably dichloromethane (DCM) in the ratio of 1:0.3-1 weight by volume to obtain an organic solution. 3. A process as claimed in claim 1, wherein the solution of Streptomycin such as hydrophilic Streptomycin is prepared in DW/NS/PBS in a ratio of 1:0.1- 100 weight by volume to obtain an aqueous solution. 4. A process as claimed in any of the preceding claims, wherein the primary emulsion is poured into 0.8-2.5% PVA solution keeping DCM: PVA ratio at 1:0.5-1.5. |
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2669-DEL-2006-Abstract-(24-11-2011).pdf
2669-DEL-2006-Claims-(17-04-2012).pdf
2669-DEL-2006-Claims-(24-11-2011).pdf
2669-DEL-2006-Correspondence Others-(17-04-2012).pdf
2669-DEL-2006-Correspondence Others-(24-11-2011).pdf
2669-DEL-2006-Correspondence-Others-(18-03-2009).pdf
2669-DEL-2006-Correspondence-Others.pdf
2669-DEL-2006-Description (Complete)-(24-11-2011).pdf
2669-del-2006-description (provisional).pdf
2669-DEL-2006-Drawings-(24-11-2011).pdf
2669-DEL-2006-Form-1-(18-03-2009).pdf
2669-del-2006-form-13-(18-03-2009).pdf
2669-DEL-2006-Form-5-(18-03-2009).pdf
2669-DEL-2006-GPA-(24-11-2011).pdf
2669-DEL-2006-PA-(18-03-2009).pdf
| Patent Number | 252495 | |||||||||
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| Indian Patent Application Number | 2669/DEL/2006 | |||||||||
| PG Journal Number | 21/2012 | |||||||||
| Publication Date | 25-May-2012 | |||||||||
| Grant Date | 18-May-2012 | |||||||||
| Date of Filing | 13-Dec-2006 | |||||||||
| Name of Patentee | LIFECARE INNOVATIONS PVT. LTD. | |||||||||
| Applicant Address | C-182, SUSHANT LOK, GURGAON, HARYANA, INDIA. | |||||||||
Inventors:
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| PCT International Classification Number | A61K31/573; A61K31/57 | |||||||||
| PCT International Application Number | N/A | |||||||||
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PCT Conventions:
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