Indian Patents
Title of Invention

"MICROCYCLIC PROLINE ANALOGUES AS HCV NS-3 SERINE PROTEASE INHIBITORS"
Abstract The present invention provides Macrocyclic proline analogues of the formula I wherein the variables are defined which inhibit NS3 protease flavi-virus such as hepatitis C virus. The compounds comprise a novel linkage between a heterocyclic P2 unit and those portions of the inhibitor more distal to the nominal cleavage site of the native substrate, which linkage reverses the orientation of peptidic bonds on the distal said relative to those proximal to the cleavage site.
Full Text HCV NS-3 Serine Protease Inhibitors
Technical Field
This invention relates to novel inhibitors of the NS3 serine protease of the flavivirus
HCV and to methods for their use in the treatment or prophylaxis of HCV.
Background Art
The NS3 serine protease of HCV is a multifunctional protein which contains a serine protease domain and a RNA helicase domain. The protease cofactor NS4A, which is a relatively small protein, is absolutely required for enhanced serine protease activity. The NS3 serine protease Is essential in the viral lifecycle. From analysis of the substrate binding site as revealed by X-ray crystal structure, it has been shown that the binding site of the NS3 protease is remarkably shallow and solvent exposed
'"'*""•:••••-
making small molecule inhibitor design a challenge.
It is believed that two HCV protease inhibitors have entered clinical trials, namely Boehringer Ingelheim's BILN-2061 disclosed in WO 0059929 and Vertex1 VX-950 disclosed in WO 0387092. A number of similarly peptidomirnetic HCV protease inhibitors have also been proposed in the academic and patent literature. Common for the vast majority of such prior art peptidomimetlcs Is the presence of an L-proline derivative at the P2 position of the inhibitor and interacting with the S2 subsite of the HCV protease enzyme. In the case of BILN-2061, the L-proline is 4-substituted with a quinoline ether, whereas VX-950 has a carboyclic ring fused to the L-proline ring. Most peptidomimetics additionally comprise additional L-amino acid derivatives peptide bonded at the P3 position, with many proposed inhibitors also including additional L-amino acid derivatives extending into P4, P5 and P6.
It has already become apparent that the sustained administration of BILN-2061 or VX-950 selects HCV mutants which are resistant to the respective drug, so called drug escape mutants. These drug escape mutants have characteristic mutations in the HCV orotease aenome, notably D168V, D168Y and/or A165S. Treatment
paradigms for HCV will thus have to resemble HIV treatment, where drug escape mutations also arise readily. Accordingly, additional drugs with different resistance patterns will consistently be required to provide failing patients with treatment options, and combination therapy with multiple drugs is likely to be the norm in the future, even for first line treatment.
Experience with HIV drugs, and HIV protease inhibitors in particular, has further emphasized that sub-optimal pharmacokinetics and complex dosage regimes quickly result in inadvertent compliance failures. This in turn means that the 24 hour trough concentration (minimum plasma concentration) for the respective drugs in an HIV regime frequently falls below the IC90 or EDgo threshold for large parts of the day. It is considered that a 24 hour trough level of at least the IC5o, and more realistically, the IC9o or EDgo is essential to slow down the development of drug escape mutants and achieving the necessary pharmacokinetics and drug metabolism to allow such trough levels provides a stringent challenge to drug design. The strongly peptidomimetic nature of prior art HCV protease inhibitors, with multiple peptlde bonds in native configurations poses pharmacokinetic hurdles to effective dosage regimes.
Brief description of] the invention
In accordance with a first aspect of the invention, there are provided compounds of
the formula I:
(Figure Removed)
wherein
A is C(=O)OR1, C(=*O)NHSO2R2, C(=O)NHR3, or CR4R4' wherein;
R1 is hydrogen, Ci-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl;
R2 is Ci-C6alkyl, Co-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl;
R3 is Ci-Cealkyl, Co-Csalkylcarbocyclyl, C0-C3alkylheterocyclyl, -OCi-C6alkyl,
-OC0-C3alkylcarbocyclyl,-OC0-C3alkylheterocyclyl;
R4 is halo, amino, or OH; or R4 and R4' together are =O;
R4' is CrCaalkyl, C0-C3alkylcarbocyclyl, C0-C3alkyiheterocyclyl;
wherein R2, R3, and R4' are each optionally substituted with 1 to 3 substituents independently selected from the group consisting of halo, oxo, nitrite, azido, nitro, Ci-CealkyI, Co-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, NH2CO-, Y-NRaRb, Y-0-Rb, Y-C(=O)Rb, Y-(C=O)NRaRb, Y-NRaC(=0)Rb, Y-NHSOpRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-C(=O)Orb and Y-NRaC(=0)ORb; Y is Independently a bond or Ci-C3a!kylene; Ra is independently H or Ci-C3alkyl;
Rb is independently H, CrC6alkyl, C0-C3alkylcarbocyclyl or C0-C3alkylheterocyclyl; p is independently 1 or 2; M is CR7R7' or NRu;
R7 is Ci-C6alkyl, CirCaalkylCs-C/cycloalkyI, or C2-C6alkenyl, any of which is optionally substituted with 1-3 halo atoms, or an amino, -SH or Co-C3alkylcycloalkyl group; or R7 is J;
R7' is H or taken together with R7 forms a Cs-Cecycloalkyl ring optionally substituted with R7a wherein;
R7'a is CrCealkyl, Cs-CscycloalkyI, C2-C6alkenyl any of which may be optionally substituted with halo; or RTa can be J; q is 0 to 3 and k is 0 to 3; where q+k £ 1;
W is -CH2-, -O-, -OC(=O)H-, -OC(=0)-r -S-, -NH-, -NRa, -NHSO2-, -NHC(=0)NH- or -NHC(=O)-, -NHC(=S)NH- or a bond;
R8 is a ring system containing 1 or 2 saturated, partially unsaturated or unsaturated rings each of which has 4-7 ring atoms and each of which has 0 to 4 hetero atoms independently selected from S, O and N, the ring system being optionally spaced
from W by a Ci-C3 alkyl group; or R8 is CrC6 alkyl; any of which R8 groups can be optionally mono, di, or tri substituted with R9, wherein
R9 is independently selected from the group consisting of halo, oxo, nitrile, azido, nitro, C-i-Cealkyl, Co-C3alkylcarbocyclyl, Co-C3alkylheterocyclyl, NH2C(=0)-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb, Y-(C=O)NRaRb, Y-NRaC(=0)Rb, Y-NHSOpRb, Y-S(-0)pRb, Y-S(=O)pNRaRb, Y-C(=O)ORb and Y-NRaC(=0)ORb; wherein said carbocyclyl or heterocyclyl moiety is optionally substituted with R10; wherein
R10 is Ci-C6alkyl, C3-C7cycloalkyl, Ci-C6alkoxy, amino, sulfonyl, (CrC3 alkyl)sulfonyl, NOz, OH, SH, halo, haloalkyl, carboxyl, amido; E is -C(=0)-, -C(=S)-, -S(=0)2-, -S(=0)-, -C(=N-Rf)-; Rf is H, -CN, -C(=O)NRaRb; -C(=O)Ci-C3alkyl;
X is -NRx- where Rx is H, d-C5aIkyl or J; or in the case where E is -C(=O), X can alsobe-O-or-NRjNRj-;
wherein one of Rj is H and the other is H, C-i-Cs alkyl or J;
R11 is H, C-i-Cealkyl, Co-Csalkylcarbocyclyl, Co-Csalkylheterocyclyl, any of which can be substituted with halo, oxo, nitrile, azido, nitro, d-Cealkyl, Co-Caalkylcarbocyclyl, Co-Csalkylheterocyclyl, NH2CO-, Y-NRaRb, Y-O-Rb, Y-C(=0)Rb, Y-(C=O)NRaRb, Y-NRaC(=O)Rb, Y-NHSOpRb, Y-S(=O)pRb, Y-S(=0)pNRaRb, Y-C(=O)ORb, Y-NRaC(=O)ORb; or R11 is J;
J, if present, is a single 3 to 10-membered saturated or partially unsaturated alkylene chain extending from the R7/R7'cycloalkyl or from the carbon atom to which R7 is attached to one of Rj, Rx, Ry or R11 to form a macrocycle, which chain is optionally interrupted by one to three heteroatoms independently selected from: -O-, -S- or -NR12-, and wherein 0 to 3 carbon atoms in the chain are optionally substituted with R14;
wherein;
R12 is H, CrC6alkyl, C3-C6cycloalkyl, orC(=0)R13;
R13 is CrCealkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl;
R14is independently selected from the group consisting of H, C-i-C6alkyl, Cr
C6haloalkyl, Ci-C6alkoxy, hydroxy, hato, amino, oxo, thio and Cr
u is independently H or d-Csalkyl;
misOor 1; n isOoM;
U is =O or is absent;
R15 is H, C-i-Cealkyl, Co-Csalkylcarbocyclyl, Co-Caalkylheterocyclyl, any of which can
be substituted with halo, oxo, nitrile, azido, nitro, Ci-Ce alkyl, Co-Caalkylheterocyclyl,
Co-C3alkylcarbocyclyl, NH2CO-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb, Y-(C=O)NRaRb, Y-
NRaC(=0)Rb, Y-NHS(=O)pRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-C(=O)ORb, Y-
NRaC(=O)ORb;
G is -O-, -NRy-, -NRjNRj-: where one Rj is H and the other is H, CrC5 alkyl or J;
Ry is H, Ci-C3 alkyl; or Ry is J;
R16 is H; or CrCealkyl, Co-Csalkylcarbocyclyl, C0-C3alkylheterocyclyl, any of which
can be substituted with halo, oxo, nitrile, azido, nitro, Ci-C6alkyl, Co-
C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, NH2CO-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb,
Y-(C=O)NRaRb, Y-NRaC(=O)Rb, Y-NHSOpRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-
C(=O)ORb, Y-NRaC(=0)ORb;
with the proviso that when m=n=0 and G is O then R16 is not tert.butyl or phenyl;
or a pharmaceutically acceptable salt or prodrug thereof.
Without in any way wishing to be bound by theory, or the ascription of tentative binding modes for specific variables, the notional concepts P1, P2, P3 and P4 as used herein are provided for convenience only and have substantially their conventional meanings, as illustrated by Schechter & Berger, (1976) Biochem Biophys Res Cornm 27 157-162, and denote those portions of the inhibitor believed to fill the S1, S2, S3 and S4 subsites respectively of the enzyme, where S1 is adjacent the cleavage site and S4 remote from the cleavage site. Regardless of binding mode, the components defined by Formula I are intended to be within the scope of the invention. For example it is expected that capping group R1B-G may interact with the S3 and S4 subsites especially when m and/or n is 0.
Various embodiments of the present invention can be notionally represented as R16-G-P4-P3-link-P2-P1, wherein P3 and/or P4 may be absent, and P1, P3 and P4 each
represents a building block constituted of a derivative of a natural or unnatural amino acid, P2 is a heterocyclic residue and G -R16 is a capping group. The link is a carbonyl or other function as defined for E. The P1 and P2 building blocks and the P3 and P4 building blocks are thus typically linked together by amide bonds whereas the P2 and P3 building blocks are linked through the above described link. The amide bonds are thereby typically reversed relative to each other on each side of the link in the compounds of the invention.
Additional aspects of the invention include a pharmaceutical composition comprising a compound of the Invention as defined above and a pharmaceutically acceptable carrier or diluent therefor.
The compounds and compositions of the invention have utility in methods of medical treatment or prophylaxis of HCV infections in humans. Accordingly, a further aspect of the invention is the use of a compound as defined above in therapy, such as in the manufacture of a medicament for the prophylaxis or treatment of flavivirus infections in humans or animals. Exemplary flavivirus include BVDV, dengue and especially HCV.
The compounds of the invention have a non-peptidic linkage at the bond between P2 and P3 building blocks, resulting In the orientation of the P3 and P4 residues being reversed relative to a native substrate. This non-peptidic link is also typically longer than the corrresponding peptide bond would have been and means that the P3 and/or P4 groups (including the R16 cap to the extent this interacts with S3 or S4) are displaced outwardly relative to a native peptide substrate. Reversal and displacement in this fashion would be expected to favour the non-natural D stereochemistries for the pocket filling groups (eg side chains) of P3 and/or P4 and/or R16. Indeed, such compounds are typically highly active and within the scope of the invention. However, it has been surprisingly found that even compounds of the invention bearing L-amino acid side chains at P3 and/or P4 exhibit good activity, notwithstanding that the respective side chain entity must approach the S3 or S4 pocket from a different angle
relative to a native peptide substrate. Accordingly L-stereochemistry at R11 and/or R16 and/or the corresponding configuration at R16 to mimic L stereochemistry represents a favoured aspect of the invention.
The different angle of approach to the S3 and/or S4 pockets also has implications for the ability of the compounds of the invention to avoid resistance patterns exhibited by prior art HCV protease inhibitors which hitherto have all had a conventional peptide backbone of natural or non-natural L-amino acid residues. As with the reverse transcriptase of HIV which is notorious for quickly generating drug escape mutants under the slective pressure of antiviral therapy, the RNA dependent RNA polymerase NS5A of HCV has a very poor proof reading capacity. This in turn means that the HCV polymerase is highly error prone and it is likely that characteristic resistance patterns will arise when HCV antivirals are administered over long periods. Even before launch, it is apparent that BILN 2061 with a substantially peptidic backbone (albeit macrocyclised) and Vertex1 NS3 protease inhibitor VX-950 with a linear peptide backbone at P3 and P4 quickly give rise to characteristic resistance mutations at positions 155, 156 or 168 of the NS3 protease (Lin et al J Biol Chem 2004 279(17):17808-17).
A preferred group of compounds of the invention comprises those wherein P1 represents a hydrazlne derivative, that Is M is NRu where Ru is typically H or Cr Caalkyl. Compounds wherein M is CR7R7' constitute a further preferred aspect of the invention.
Preferred embodiments wherein M is CR7R7' in formulae I include formulae IA:

(Figure Removed)
Preferred values for q and k in Formula I include 2:1, 2:2,2:3, 3:2,3:3, more preferably 1:2 and 1:0; and most preferably 1:1, In which case preferred compounds have the partial structure:
(Figure Removed)
where e is 1 or 2.
It is currently preferred that E is -C(=0)- or -C=N-Rf, for example where Rf is -CN or-C(=0)NH2.
Compounds of the invention may comprise both a P3 and a P4 function, viz m and n are each 1. Favoured embodiments within formula I Include formula Ida-Idd below:
(Figure Removed)
Alternative embodiments Include the structures corresponding to Ida, Idb, Idc and Idd wherein M is NRu.
Alternative configurations of the compounds of the invention comprise a P3, but no P4 function, viz m is 1 and n is zero. Preferred embodiments within Formula I include formulae lea-lee below:
(Figure Removed)
Alternative embodiments include the structures corresponding to lea, leb, lec, led and lee wherein M is NRu.
Still further alternative configurations of the compounds of the invention include those where m and n are zero and thus groups R16-G abut P2, but as mentioned above, the capping group R16-G may interact favourably with S3 and/or S4.
Favoured embodiments within Formula I include formulae Ifa-lfe below:
(Figure Removed)
R18 in figure Ifb and elsewhere is typically H, Ci-Caalkyl, C6-C6alkyl, C0-Caalkylheterocyclyl, Ci-Csalkyicarbocyclyl or Ca-Crcycloalkyl, any of which being optionally substituted, as described above. For example, R16 ca be phenyl substituted as described above.
Alternative embodiments include the structures corresponding to Ifa, Ifb, Ifc, Ife, and Ife wherein M is NRu.
The compounds of the invention may comprise linear molecules, as depicted above. Alternatively, in embodiments wherein R7 and R7' together define a spiro cycloalkyl group, such as spiro-cyclopropyl, the compounds of the invention may be configured as macrocycles, wherein a linking group J extends between one of Rj, Rx, Ry or R11 of formula I. Alternatively the macrocycle J may extend from the carbon adjacent to R7 to one of Rj, Rx, Ry or R11.
Favoured embodiments of such macrocyclic structures within formula I wherein m is 0 and n is 1 include those of Formulae Iga-lgd below:
(Figure Removed)
The corresponding structures wherein the J chain bonds to the carbon adjacent R7 are also favoured.
ditional favoured embodiments of such macrocyclic structures within formula wherein m is 0 and n is 1 include those of Formulae Ige-lgf below:
(Figure Removed)
The corresponding structures wherein the J chain bonds to the carbon adjacent R7 are also favoured
Favoured macrocyclic structures within Formula I, comprising both a P3 and P4 function, ie wherein m and n are each 1, include those of the formulae Iha-lhd below.

(Figure Removed)
The corresponding structures wherein the J chain bonds to the carbon adjacent R7
are also favoured.
Favoured macrocyclic structures within Formula I, wherein both of the P3 and P4 functions are absent, i.e. wherein m and n are each 0, include those of the formulae Ihe-lhh below, especially 1he and 1hf.

(Figure Removed)
The corresponding structures wherein the J chain bonds to the carbon adjacent R7 are also favoured, especially formula Ihe and 1hf:
In general, in the optionally macrocyclic structures such as those illustrated above, linker J is a 3 to 10 chain atom, preferably 5 to 8 chain atom, such as 6 or 7 chain atom, saturated alkylene chain or a partially unsaturated alkylene chain, that is an alkylene chain bearing 1 to 3 unsaturated bonds between adjacent carbons, typically one unsaturation. The length of the chain will, of course, depend on whether J extends from Rd, Rj, Rx, Ry, R11 or the carbon adjacent to R7, Suitable chains are described in detail in WO 00/59929. Typically J will be dimensioned to provide a macrocycle of 13 to 16 ring atoms (including those atoms in the P1, P2 and if present P3 groups contributing to the ring). Conveniently J is dimensioned to provide a macrocycle of 14 or 15 ring atoms.
Conveniently, the J chain contains one or two heteroatoms selected from: O, S, NH, NCi-C8 alkyl or N-C(=O)Ci-C6alkyI. More preferably, the J chain optionally contains one heteroatom selected from: NH, or N-C(=O)Ci-Cealkyl, most preferably N(Ac). Most preferably, the chain containing a nitrogen atom is saturated. In an alternative embodiment, J contains one heteroatom selected from O or S. The chain may be substituted with R14, such as H or methyl.
Typically, the J linker structure is saturated. Alternatively, J contains 1 to 3, preferably 1 double bond, typically spaced one carbon from the cycloalkyl R7 function, if present. The double bond may be cis or trans.
Representative examples of J thus include pentylene, hexylene, heptylene, any of which are substituted with Ci-C6a!kyl, Ci-C6haloalkyl, CrCealkoxy, hydroxyl, halo, amino, oxo, thio or C-i-Ce thioalkyl; penten-3-yl, hexen-4-yl, hepten-5-yl, where 3, 4 or 5 refers to a double bond between carbon atoms 3 and 4,4 and 5 etc.
Convenient R7 and Rr groups include those wherein Rr is H and R7 is n-ethyl, n-propyl, cyclopropylrnethyl, cyclopropyl, cyclobutylmethyl, cyclobutyl, 2,2-difluoroethyl, or mercaptomethyl. Preferred embodiments include those wherein R7 is n-propyl or 2,2-difluoroethyi.
Alternative favoured configurations for R7 and R7' include those wherein R7' is H and R7 is C3-C7 cycloalkyl or Ci-C3alkylC3-C7cycloalkyl.
Still further favoured configurations for R7 and R7' Include these wherein R7' is H and R7 is J.
Alternatively, R7 and R7" together define a spiro-cycloalkyl function, such as a spiro-cyclobutyl ring, and more preferably a spiro-cyclopropyl ring. "Spiro" in this context simply means that the cycloalkyl ring shares a single carbon atom with the peptidic backbone of the compound. The ring is substituted or unsubstituted. Preferred
substituents include mono or di-substitutions with R7'a wherein R7ia is Ci-Ce alkyl, C3-Cgcycloalkyl, orCz-Ce alkenyl, any of which is optionally substituted with halo. Alternatively the substituent may be a J linker as described above. Currently preferred stereochemistries for a spiro-cyclopropyl ring are defined below.
Particularly preferred substituents include R7a as ethyl, vinyl, cyclopropyl (ie a spiro-cyclopropyl substituent to the "spiro" cycloalkyl ring of R7/R7'), 1- or 2-bromoethyl, 1-or 2-fluoroethyl, 2-bromovinyl or 2-fluorethyl..
In one embodiment of the invention, A is -CR4R4' as illustrated in detail in PCT/EP03/10595, the contents of which are incorporated by reference.
Convenient R4' groups thus include Ci-C6alkyl, such as methyl, ethyl, propyl, ethenyl and -CHCHCHs. Alternative preferred R4' groups include aryl or heteroaryl such as optionally substituted phenyl, pyridyl, thiazolyl or benzimidazolyl or CrC3alkylaryl or Ci-C3alkylheteroaryl, where the alkyl moiety is methyl, ethyl, propyl, ethenyl and -CH=CHCH3. Preferred aryl moieties include optionally substituted: phenyl, benzothiazole and benzimidazole.
Favoured R4 groups include -NHa, fluoro or chloro. Alternative preferred R4 groups include -OH and especially =O.
An alternative embodiment for A is C(=O)NHR3, where R3 is optionally substituted C0-C3alkylaryl, Co-C3alkylheteroaryl, OC0-C3alkylaryl or OCo-Caalkylheteroaryl. Appropriate substituents appear in the definitions section below.
A currently favoured configuration for A is C(=O)OR1, especially where R1 is d-l, such as methyl, ethyl, or tert-butyl and most preferably hydrogen.
A particularly preferred configuration for A is C(=O)NHSO2R2, especially where R2is optionally substituted CrCealkyl, preferably methyl, or optionally substituted C3-
Crcycloalkyl, preferably cyclopropyl, or optionally substituted Co-C6alkylaryl, preferably optionally substituted phenyl. Appropriate subbstituents appear in the definitions section below.
Substituent -W-R8 on the cyclic P2 group can employ any of the praline substituents which are extensively described in WO 00/59929, WO 00/09543, WO 00/09558, WO 99/07734, WO 99/07733, WO 02/60926, WO03/35060, WO 03/53349, W003/064416, W=Q3/66103, WO03/064455, W003/064456, WO03/62265, W003/062228, WO03/87092, WO 03/99274, W003/99316, WO03/99274, W004/03670, , WO04/032827, WO04/037855, WO04/43339, WO04/92161, W004/72243, 5WO04/93798. W004/93915, WO04/94452, WO04/101505, W004/101602, WO04/103996, WO04113365 and the like.
Favoured W functions include W as -OC(=O)NH-, -OC(=O)-, -NH-, -NR8'-, -NHS(O)2-or -NHC(=O)-, especially -OC(=O)NH- or -NH-. Favoured R8 groups for such W functions include optionally substituted Co-Caalkylcarbocyclyl or Co-C3alkyl-heterocyclyi, including those described in WO0009543, WO0009558 and WO 00/174768. For example ester substituents, -W-R8, on the cyclic P2 group, include those disclosed in WO 01/74768 such as Ci-Csalkanoyloxy, Co-C3alkylaryloyloxy, particularly (optionally substituted) benzoyloxy or Co-C3alkylheterocycloyloxy, especially
(Figure Removed)
This publication also describes alternative -W-R8 possibilities for example Ci-C6alkyl, such as ethyl, isopropyl, C0-C3alkyicarbocyclyl such as cyclohexyl, 2,2-difluoroethyl, -C(=O)NRc, where Re is Ci-C6 alkyl, C0-C3alkylcyclopropyl, C0-C3alkylaryl or C0-Csalkylheterocyclyl.
Currently preferred W functions include -S- and especially -O-. Convenient values for R8 in such embodiments include Co-Csalkylaryl, or C0-C3alkylheteroaryl either of which is optionally mono, di, or tri substituted with R9, wherein;
R9 is Ci-Ce alkyl, CrC6alkoxy, NOz, OH, halo, trifluoromethyl, amino or amido (for example amido or amino optionally mono- or di-substituted with Ci-Caalkyl), Co-Csalkylaryl, Co-Csalkylheteroaryl, or carboxyl, wherein the aryl or heteroary! moiety is optionally substituted with R10; wherein
R10 is CrCealkyl, C3-C7cycloalkyI, CrCealkoxy, amino, amido, sulfonylCr C3alkyl, NOa, OH, halo, trifluoromethyl, carboxyl, or heteroaryl.
Typically, the Co-C3 alkyl component of R8 as Co-Csalkylaryl, or Co-C3alkylheteroaryl is methyl and especially absent, ie C0. The aryl or heteroaryl component is as extensively illustrated in the definition section below.
Preferred R9 include CrC6 alkyl, CrCealkoxy, amino, (such as di-CrC3alkylamino), amido (such as -NHC(0)CrC6alkyl or C(=O)NHC1-C6alkyl), aryl or heteroaryl, the aryl or heteroaryl being optionally substituted with R10; wherein
R10 is CrC6alkyl, Ca-Crcycloalkyl, Ci-Cealkoxy, amino,(such as mono- or di-CrCa alkylamino), amido (such as as-NHC(O)CrC3alkyl or C(=O)NHCrC3alkyl), halo, trifluoromethyl, or heteroaryl.
Preferred R10 include CrCealkyl, d-Cealkoxy, amino, amido (such as as-NHC(0)d-C6alkyl or C(=O)NHCrC6alkyl), halo, or heteroaryl.
Particularly preferred R10 include methyl, ethyl, isopropyl, tert-butyl, methoxy, chloro, amino, amido (such as as -NHC(O)CrCBalkyl, for example -NC(=O)CHC(CH3)3, or C(=O)NHCrC3alkyl) or CrC3alkyl thiazole.
Favoured embodiments of R8 include 1-naphthylmethyl, 2-naphthylmethyl, benzyl, 1-naphthyl, 2-naphthyl, orquinolinyl, any of which is unsubstituted, mono, or
disubstituted with RB as defined, in particular 1-naphthylmethyl, or quinolinyl unsubstituted, mono-, or disubstituted with R9 as defined.
A currently preferred R8 is:
(Figure Removed)

wherein R9a is CrCealkyl; Ci-C6alkoxy; thioCrC3alkyl; amino optionally substituted with Ci-Csalkyl; Co-Csalkylaryl; or Co-C3alkylheteroaryl, Co-Caalkylheterocyclyl, said aryl, heteroaryl or heterocycle being optionally substituted with R10 wherein
R10 is d-Cealkyl, Ca-Crcycloalkyl, C-pCealkoxy, arnino, amido, heteroaryl or
heterocyclyl; and
R9b is CrC6alkyl, CrC6alkoxy, amino, arnido, NO2, OH, halo, trifluoromethyl, carboxyl.
Convenient R6a include aryl or heteroaryl, all optionally substituted with R10 as defined, especially where R9a is selected from the group consisted of:
(Figure Removed)
wherein R10 is H, CrC6alkyl, or Co-Csalkyl-Cs-Cecycloalkyl, amino optionally mono- or di-substituted with CrC6alkyl, amido (such as as -NHC(O)Ci-C6alkyl or C(=O)NHCi-Cealkyl), heteroaryl or heterocyclyl.
R9a is conveniently phenyl and thus R8 is:
(Figure Removed)
wherein R10a is H, CrC6alkyl; Ci-C6alkoxy; or halo; and R9fa is CrC6 alkyl, CrC6-alkoxy, amino such as di(CrC3alkyl)amine, amido (such as as -NHC(0)CrC3alkyl or C(=O)NHCrC3alkyl), N02, OH, halo, trifluoromethyl, carboxyl.
An alternative preferred R8 is:
(Figure Removed)
wherein R10a is H, d-Cealkyl, or Co-C3alkyl-C3-C6cycloalkyl, amine (such as amine mono- or di-substituted with Ci-C6alkyl), amido (such as as-NHC(O)CrC6alkyI or C(=O)NHCrC6alkyl), heteroaryl or heterocyclyl; and R9b is Ci-C6 alkyl, Ci-C6-alkoxy, amino (such as di(Ci-C3 alkyl)amino), amido (such as as - NHC(O)Ci-C3alkyl or C(=O)NHCrC3alkyl), N02, OH, halo, trifluoromethyl, or carboxyl.
In the immediately above described embodiments R9b is conveniently CrCe-alkoxy, preferably methoxy.
A further convenient R8, for example when W is an ether, has the formula
(Figure Removed)
where W is N or CH, r is 0 or 1, Ra' is H, CrC6 alkyl, C0-C3alkylcycloalkyl, Cr C6alkyloxy, hydroxy or amine and Rb' is H, halo, CrC6alkyl, C0-C3alkylcycloalkyl, Cr Cealkyloxy, d-Cethioalkyl, cycloalkylCo-Csalkyloxy, CrCsalkyloxyCi-Caalkyl, C0-C3alkylaryl or C0-C3alkylheterocyciyl. A particularly preferred ether substituent is 7-methoxy-2-phenyl-quinolin-4-yl oxy.
When W is a bond then R8 is preferably a substituted or unsubstituted heterocyclic ring system as described in WO2004/072243 or W02004/113665,
Representative examples of R8 when W is a bond include the following aromatics which may optionally be substituted: 1H-pyrrole, 1 H-imldazole, 1 H-pyrazote, furan, thiophene, oxazole, thiazole, isoxazole, isothiazole, pyridine, pyndazine, pyrimidine, pyrazine, phthalazine, quinoxaline, quinazoline, quinoline, cinnoline, 1H-pyrrolo[2,3]-6]pyridine, IH-indole, 1H-benzoimidazole, 1W-indazole, 7W-purine, benzothiazole, benzooxazole, 1 W-lmidazo[4,5-c]pyridine, 1H-imidazo[4I5-&]pyridineI 1,3-dihydro-benzofmidazoI-2-one, 1, 3-dihydro-benzoimidazol-2-thione, 2,3-dihydro~1/-/-indole, 1,3-dihydro-indol-2->one, 1 H-indole-2,3-dione, 1,3-dihydro-benzoimidazole-2-one, V-l, 1H-pyrrolo [2, 3-cJpyridine, benzofuran, benzo[6]thiophene, benzotdjisoxazole, benzo[cOisothiazole, 1 H-quinotin-2-one, 1 H-quinolin-4-one, 1H-quinazolin-4-one, 9H-carbazole, 1 H-quinazolin-2-one.
Additional representative examples of R8 when W is a bond, include the following non-aromatics, which may be optionally subsbstituted: aziridine, azetidine, pyrrolidine, 4,5-dihydro-1H-pyrazole, pyrazolidine, imidazolidin-2-one, imidarolidine-2-thione, pyrrolidin-2-one, pyroIidine-2,5-dione, piperidine-2,6-dione, piperidin-2-one, piperazine-2,6-dione, plperazin-2-one, piperazine, morphollne, thiomorpholine-1,1-dioxide, pyrazolidin-3-one, imidazolidine-2,4-dione, piperidine, tetrahydrofuran, tetrahydropyran, [1,4]dioxane, 1,2,3,6-tetrahydropyridlne.
Preferred values for R8 when W is a bond, include tetrazole and derivatives thereof. The tetrazole moiety is linked to the cyclic P2 scaffold and optionally substituted as shown below:
(Figure Removed)
wherein Q* Is selected from the group consisting of absent, -CH2-, -O-, -NH-, -N(R1*), -S-, -S(=0)a- and -(C=0)-; Q* is selected from the group consisting of: absent, -CH2-and -NH; Y* is selected from the group consisting of: H, d-Cealkyl, C0-C3aryl, C0-C3heterocyclyl; Rr is selected from the group consisting of: H, Ci-Cealkyl, carbocyclyl, C0-C3aryl, C0-C3heterocyclyl,
Representative examples of substituted tetrazoles are as described in table 1 of WO2004/072243 and the structures following immediately after, or WO2004/113665.
Further preferred values for R8 when W is a bond, Include triazole and derivatives thereof. The triazole moiety is linked to the cyclic P2 scaffold and optionally substituted as shown below:

(Figure Removed)
wherein X* and Y* are independently selected from the group consisting of: H, halogen, Ct-C6alkyl, Co-C3carbocyclyl, -CH2-amlno, -CH2-arylamlno, -CH2-diarylamino, -(C=0)-amino, -(C=0)-aryiamino, -(C=O)-diarylamino, C0-C3aryl, C0-Caheterocyclyl or alternatively, X* and Y* taken together with the carbon atoms to which they are attached, form a cyclic moiety selected from the group consisting of aryl and heteroaryl.
Representative examples of substituted triazoles are as described in table 2 of WO2004/072243 and the structures following immediately after, and in the tables of W02004/113365.
Further preferred values for R8 when W is a bond, include pyridazinone and derivatives thereof. The pyridazinone moiety is linked to the cyclic P2 scaffold and optionally substituted as shown below:

(Figure Removed)
wherein X*, Y* and Z* are independently selected from the group consisting of: H, N3, halogen, Ci-C6allcyl, carbocyclyl, amino, Co-C3aryl, -S-aryl, -O-aryl, -NH-aryl, diarylamino, diheteroarylamlno, C0-C3heterocyclyl, -S-heteroaryl, -0-heteroaryl, NH-heteroaryl or, alternatively, X and Y or Y and Z taken together with the carbon atoms to which they are attached, form an aryl or heteroaryl cyclic moiety.
Representative examples of substituted pyridazinones are as described in table 3 of WO2004/072243 and the structures following immediately after, and in the tables of W02004/113365.
Preferred P3 groups, i.e. when m is 1, resemble natural or unnatural amino acids, especially aliphatic amino acids, such as L-valyl, L-leucyl, L- isoleucyl or L- t-leucyl. Further preferred P3 groups, as shown in WO 02/01898 include C0-C3alkylcycloalkylalanine, especially cyclohexylalanine, optionally substituted with CO2Rg, where Rg is H, is Ci-C6alkyl, Co-C3alkylaryl, Co-C3alkylheterocyclyI, C0-C3alkylcycloalky[ or amine; or N-acetylpiperidine or tetrahydropyran. Preferred R11 groups thus include Ct-Cealkyl, C0-C3alkylcarbocyc!yl for example Co-C3alkylC3-Crcycloalkylyl, Co-Csalkylaryl or Co-C3alkylheteroaryl, any of which is optionally substituted with hydroxy, halo, amino, CrC5alkoxy, CrC6thioalkyl, C(=0)OR14, carboxyl, (CrC6aIkoxy)carbonyl, aryl, heteroaryl or heterocyclyl, especially where the substituent is hydroxy or C(=0)OR14.
Particularly preferred R11 include tert-butyl, iso-butyl, cyclohexyl, phenylethyl, 2,2-dimethyl-propyl, cydohexylmethyl, phenylmethyl, 2-pyridylmethyl, 4-hydroxy-phenylmethyl, or carboxylpropyl. The most preferred R11 values are currently tert-butyl, isobutyl, or cyclohexyl.
An embodiment of the invention include compounds wherein P4 is absent (ie n is 0) and wherein the P3 function lacks a carbonyl, ie U is absent. Representative substructures include those of formula li below:
(Figure Removed)
wherein
Rx and Ry are as defined above, preferably H,
R11' is CrC6 alkyl, preferably C3-C5 branched alkyl such as the side chains of L-valyl, L-leucyl, L-isoleucyl, L-t-leucyl; or Co-CaalkylCa-Cr cycloalkyl such as cyclohexyl or cyclohexylmethyl;
R16a is -Rba, -S(=O)pRba, -C(=0)Rba; Rba is Ci-C6 alkyl, C0-C3alkylheterocyclyl, Co-Caalkylcarbocyclyl. Alternatively, compounds of partial structure li may be macrocyclised between an appropriate value of R7 and one of Rx, Ry or R11>.
Representative embodiments of P3 groups which lack a carboxy function (ie variable U is absent) include those of formula lia-lid below:

(Figure Removed)
where Ar is carbocyclyl or heterocyclyl, especially aryl or heteroaryl, any of which is optionally substituted with R9. Although the partial structures of Formulae lia - lid have been illustrated in the context of a compound within Formula I, it will be apparent that such configurations of Formula li apply also to other values of q and k. Similarly, although the partial structures of formulae lie and lid show an R11 group corresponding to leucine, it will be apparent that these configurations will be applicable to other R11 groups, especially those resembling the side chains of natural or unnatural L-amino acids, for example t-butyl alanine/t-leucine.
R15 in those compounds of the invention wherein n is 1, is preferably optionally substituted Ci-Cealkyl or Co-Caalkylcarbocyclyl for example Co-CsalkylCa-Crcycloalkyl, any of which may be optionally substituted. Preferred P4 groups are typically analogues of natural or unnatural amino acids, especially aliphatic amino acids such as L-valyl, L-leucyl, L-isoleucyl, L-t-Ieucyl or L-cyclohexylalanine and thus favoured R15 groups include cyclohexyl, cyclohexylmethyl, tert-butyl, iso-propyl, or iso-butyl.
Preferred G values include -NRy-, especially wherein Ry is methyl or preferably H, or hydrazine.
further preferred G value is 0 thereby defining an ester with the carbonyl of P4 (if present) or the carbonyl of P3 (if present) or an ether in the case of variants wherein group U is absent. Conventional pharmaceutically acceptable ethers or esters capping groups for R1S include C^Cealkyl (especially methyl art-butyl), cq-C3alkylheterocyclyl (especially pyridyl, benzimldazolyl, plperidyl, morpholinyl, piperazinyl) or Co-C3alkylcarbocyclyl (especially phenyl, benzyl, indanyl) any of which is optionally substituted with hydroxy, halo, amino, or Ci-Cealkoxy.
It will be apparent that for compounds of formula I, when nv=n=0, then R16G- is not a BOG or CBz protecting group, but this restriction does not apply to other permutations of m and n. The Boo or CBz protected-4-substituted proline synthetic intermediates described for example in WO 0059929 are thus outside the scope of the invention.
Favoured compounds of the Invention can comprise a hydrazine functionality, for example where X is -NHNH- and m is 1; with n being zero or 1. Alternatively, especially where m is zero, G can be -NRjNRj- such as -NHNH-. Compounds will generally not comprise a hydrazine at both G and X. Typical hydrazines within Formula I, wherein m and n are zero include compounds of the partial structures Ija-Ijb below:

(Figure Removed)
R16 in formulae Ija and Ijb can be regarded as an alkyl (or CrCo-alkyiheterocyclyl or Ci-Cgalkyl carbocyclyl) wherein the first alkyl carbon is substituted with an oxo group to define the keto function and R16r is the remainder of the alkyl, alkylheterocyclyl or alkylcarbocyclyl moiety. Formula Ijb depicts a variant where R1S is a methylene group whose carbon is substituted with an oxo substituent and also -ORb, where Rb is as
defined above, typically, Ci-Ce alkyl, such as t-butyl, C0-C3alkylheterocyclyl such as pyridyl, or Co-Caalkylcarbocyclyl, such as benzyl or phenyl, any of which is optionally substituted as defined above. Compounds of partial structures Ija and ijb can be linear molecules as shown (both Rj are H), or preferably one of the depicted Rj groups can be macrocyclised via J to an appropriate R7 group.
Alternative hydrazines of Formula I where m is 1 include those of partial structures Ijc and Ijd below:
(Figure Removed)
where R16, G, R11, R16, Rj and Ru are as defined for formula I above. Compounds of partial structures Ijc and Ijd can be linear molecules as shown (both Rj are H), or preferably one of the depicted Rj groups, or the R11 group can be macrocyclised via J to an appropriate R7 group
Although formulae Ija-ljd are depicted with a proline analogue as P2, it will be apparent that this aspect of the invention is equally adapted to other configurations of q and k.
Alternative hydrazine-like configuration are found when G is amino, and m and n are 0, and R16 is an N-linked unsaturated heterocyde as defined below, for example pyridyl or pyrimidyl or a saturated heterocycle as defined below, such as piperazinyl, piperidinyl and especially morpholinyl. Examples of such embodiments include those of the formulae Ije:
(Figure Removed)
Compounds of partial structures Ije can be linear molecules as shown or preferably Rx can be macrocyclised via J to an appropriate R7 group. Although these partial structures are depicted with a five membered ring for P2, It will be readily apparent that this configuration extends to other values of q and k. Similarly these configurations will be applicable to other N-linked heterocycles as R16.
Returning now to Formulae I in general, favoured R16 groups for the compounds of the invention include 2-indanol, indanyl, 2-hydroxy-1-phenyl-ethyl, 2-thiophenemethyl, cyclohexylmethyl, 2, 3-methyIenedioxy benzyl, cyclohexyl, phenyl, benzyl, 2-pyridylmethyl, cyclobutyl, iso-butyl, n-propyl, methyl, or 4-methoxyphenylethyl.
Currently preferred R1e groups include 2-indanol, indan, 2-hydroxy-1-phenyl-ethyl, 2-thiophenemethyl, 2,3-methylenedioxybenzyl, or cyclohexylmethyl.
Unnatural amino acids include L-amino acids wherein the side chain is not one of the 20 naturally occurring amino acids. Examples of non-natural amino acids include L-beta-methylsulfonylmethylalanine, L-cyclohexylalanine, L-tertiary-leucine, L-norleucine, L-norvaline, L-ornithine, L-sarcosine, L-citurline, L-homophenylalanine, L-homoserine, L-beta-(1-napthyl)alanine, L-beta-(2-napthyl)alanine etc. Non natural amino acids also include the D-amlno acids corresponding to the 20 natural amino acids and D-amino acids bearing other side chains, such as those listed above.
P (also abbreviated as CrCealk, or used in compound expressions such as etc) as applied herein is meant to include straight and branched chain
aliphatic carbon chains such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, pentyl, isopentyl, hexyl, heptyl and any simple isomers thereof. The alkyl group may have an unsaturated bond. Additionally, any C atom in CrC6alkyl may optionally be substituted by one, two or where valency permits three halogens and/or substituted or the alkyl chain interrupted by a heteroatom S, 0, NH. If the heteroatom is located at a chain terminus then it is appropriately substituted with one or 2 hydrogen atoms. CrC4alkyl and Ci-C5alkyl have the corresponding meaning to Ci-Caalkyl adjusted as necessary for the carbon number.
'Ci-C3alkyl' as applied herein includes methyl, ethyl, propyl, Isopropyl, cyclopropyl, any of which may be optionally substituted or heteroatom interrupted as described in the paragraph above or in the case of Czor C3, bear an unsaturated bond such as CH2=CH.
"Ci-C3alkylene" as applied herein describes a divalent CfCaalkyidiyl moiety, including propylene, ethylene and especially methylene. The typically longer alkylene chains for J may comprise 1 to 3 unsaturations and/or interruptions with heteroatoms as defined above.
'Amino' includes NHa, NHCi-Cealkyl or N(CrCe-alkyl)2, especially CrC3 alkyl variants
'Amido' includes C(=O)NH2, and alkylamido, such as C(=O)NHCi-C6alkyl, C(=O)N(C.rC6alkyl)2 especially C(=0)NHd-C3alkyl, C(=O)N(CrC3alkyl)2 or -NH(C=0)Ci-Cfialkyl, for example -NHC(=O)CHC(CH3)3| including -NH(OO)C,-C3alkyl. .
'Halo' or halogen as applied herein is meant to include F, Cl, Br, I, particularly chloro and preferably fluoro.
'Co-C3alkylaryr as applied herein is meant to Include an aryl moiety such as a phenyl, naphthyl or phenyl fused to a C3-C7cycloalkyl (for example indanyl), which aryl is
directly bonded (I.e. C0) or through an Intermediate methyl, ethyl, or propyl group as defined for Ci-C3alkylene above. Unless otherwise indicated the aryl and/or its fused cycloalkyl moiety is optionally substituted with 1-3 substituents selected from halo, hydroxy, nitro, cyano, carboxy, C-i-Cealkyl, C-i-Csalkoxy, Ci-Cealkoxyd-Cealkyl, C-i-Cgalkanoyl, amino, azido, oxo, mercapto, nitro Co-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl. "Aryl" has the corresponding meaning, i.e. where the Co-C3alkyl linkage is absent.
'Co-C3alkylC3C7cycloalkyl' as applied herein is meant to include a Cs-Cycycloalkyl group such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl, which cycloalkyl is directly bonded (i.e. Coalkyl) or through an intermediate methyl, ethyl, proyl or isopropyl group as defined for CrC3alkylene above. The cycloalkyl group may contain an unsaturated bond. Unless otherwise indicated the cycloalkyl moiety is optionally substituted with 1-3 substituents selected from halo, hydroxy, nitro, cyano, carboxy, CrC6alkyl, CrC6alkoxy, CrCealkoxyCrCealkyl, Ci-C6alkanoyl, amino, azido, oxo, mercapto, nitro Co-C3alkylcarbocyclyl, Co-C3alkylheterocyclyl.
'Co-Caalkylcarbocyclyl1 as applied herein is meant to include Co-C3alkylaryl and Co-CaalkylCs-C/cycloalkyl. Unless otherwise indicated the aryl or cycloalkyl group is optionally substituted with 1-3 substituents selected from halo, hydroxy, nitro, cyano, carboxy, Ci-C6alkyl, C-i-Cealkoxy, CrCealkoxyd-Cealkyl, Ci~C6alkan6yl, amino, azido, oxo, mercapto, nitro, C0-C3alkylcarbocyclyl and/or Co-C3alkylheterocyclyl. "Carbocyclyl" has the corresponding meaning, i.e. where the Co-C3alkyl linkage is absent
'Co-Caalkylheterocycylyl' as applied herein is meant to include a monocyclic, saturated or unsaturated, heteroatom-containing ring such as piperidinyl, morpholinyl, piperazinyl, pyrazolyi, imidazolyl, oxazolyl, isoxazolyl, thiazinolyl, isothiazinolyl, thiazolyl, oxadiazolyl, 1,2,3-triazolyl, 1,2,4-triazolyl, tetrazolyl, furanyl, thienyl, pyridyl, pyrimidyl, pyridazinyl, pyrazolyi, or any of such groups fused to a phenyl ring, such as quinolinyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzothiazinolyl,
benzisothiazinolyl, benzothiazolyl, benzoxadiazolyl, benzo-1,2,3-triazolyl, benzo-1,2,4-triazolyl, benzotetrazolyl, benzofuranyl, benzothienyl, benzopyridyl, benzopyrimidyl, benzopyridazinyl, benzopyrazolyl etc, which ring is bonded directly i.e. (Co), or through an intermediate methyl, ethyl, propyl, or isopropyl group as defined for Ci-C3alkylene above. Any such non-saturated rings having an aromatic character may be referred to as heteroaryl herein. Unless otherwise indicated the hetero ring and/or its fused phenyl moiety is optionally substituted with 1-3 substituents selected from halo, hydroxy, nitro, cyano, carboxy, C-i-Caalkyl, Cr Cealkoxy, CrCealkoxyC-i-Cealkyl, Ci-C6alkanoyl, amino, azido, oxo, mercapto, nitro, Co-Csalkylcarbocyclyl, C0-C3alkylheterocyclyl. "Heterocyclyl" and "Heteroaryl" have the corresponding meaning, i.e. where the Co-Csalkyl linkage is absent.
Typically heterocycyl and carbocyclyl moieties within the scope of the above definitions are thus a monocyclic ring with 5 or especially 6 ring atoms, or a blcyclic ring structure comprising a 6 membered ring fused to a 4, 5 or 6 membered ring.
Typical such groups include Ca-Cacycloalkyl, phenyl, benzyl, tetrahydronaphthyl, indenyl, indanyl, heterocyclyl such as from azepanyl, azocanyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, indolinyl, pyranyl, tetrahydropyranyl, tetrahydrothlopyranyl, thiopyranyl, furanyl, tetrahydrofuranyl, thienyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, imidazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, tetrazolyl, pyrazolyl, indolyl, benzofuranyl, benzothienyl, benzimidazolyl, benzthiazolyl, benzoxazolyl, benzisoxazolyl, quinolinyl, tetrahydroquinolinyl, isoquinolinyl, tetrahydroisoquinolinyl, quinazolinyl, tetrahydroquinazolinyl and quinoxallnyl, any of which may be optionally substituted as defined herein.
The saturated heterocycle moiety thus includes radicals such as pyrrolinyl, pyrrolidinyl, pyrazolinyl, pyrazolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, pyranyl, thiopyranyl, piperazinyl, indolinyl, azetidinyl, tetrahydropyranyl, tetrahydrothiopyranyl, tetrahydrofuranyl, hexahydropyrimidinyl, hexahydropyridazinyl,
1,4,5,6-tetrahydropyrimidlnylamlne, dihydro-oxazolyl, 1,2-thiazinanyl-1,1-dioxide, 1,2,6-thiadiazinanyl-1,1 -dioxide, isothiazolidinyl-1,1 -dioxide and imidazolidinyl-2,4-dione, whereas the 'unsaturated heterocycle include radicals with an aromatic character such as furanyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazoiyl, isoxazolyl, isothiazolyl, oxadiazolyi, triazolyl, tetrazolyl, thiadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolfzinyl, indolyl, Isoindolyl. In each case the heterocycle may be condensed with a phenyl ring to. form a bicyclic ring system.
Synthesis
Synthesis of the compounds of the present invention can be performed by different chemical strategies in solution or solid phase or a combination of both. The suitably protected individual building blocks can first be prepared and subsequently coupled together i.e. P2+P1-*- P2-P1. Alternatively, precursors of the building blocks can be coupled together and modified at a later stage of the synthesis of the inhibitor sequence. Further building blocks, precursors of building blocks or prefabricated bigger fragments of the desired structure, can then be coupled to the growing chain, e.g. R16-G-P3+ E-P2-P1-*- R16-G-P3-P2-P1 or R16-G-P4-P3+E-P2-P1-> R16-G-P4-P3-E-P2-P1.
Coupling between two amino acids, an amino acid and a peptide, or two peptide fragments can be carried out using standard coupling procedures such as the azide method, mixed carbonic-carboxylic acid anhydride (isobutyl chloroformate) method, carbodiimide (dicyclohexylcarbodiimide, diisopropylcarbodiimide, or water-soluble carbodiimide) method, active ester (pnitrophenyl ester, N-hydroxysuccinic imido ester) method, Woodward reagent K-method, carbonyldiimidazole method, phosphorus reagents or oxidation-reduction methods. Some of these methods (especially the carbodiimide method) can be enhanced by adding 1-hydroxybenzotriazole or 4-DMAP. These coupling reactions can be performed in either solution (liquid phase) or solid phase.
More explicitly, the coupling step involves the dehydrative coupling of a free carboxyl
me reactant with the free amino group of the other reactant in the present of a coupling agent to form a linking amide bond. Descriptions of such coupling agents are found in general textbooks on peptide chemistry, for example, M. Bodanszky, "Peptide Chemistry", 2nd reved., Springer-Verlag, Berlin, Germany, (1993) hereafter simply referred to as Bodanszky, the contents of which are hereby incorporated by reference. Examples of suitable coupling agents are N,N'-dicyclohexylcarbodiimide, 1-hydroxybenzotriazole in the presence of N,N'- dicyclohexylcarbodiimide or N-ethyl-N'- [ (Sdimethylamino) propyl] carbodiimide. A practical and useful coupling agent is the commercially available (benzotriazol-1-yloxy) tris- (dimethylamino) phosphonium hexafluorophosphate, either by itself or in the present of 1-hydroxybenzotriazole or 4-DMAP, Another practical and useful coupling agent is commercially available 2-(IH-benzotriazol-1-yl)-N, N, N',N'- tetramethyluronium tetrafluoroborate. Still another practical and useful coupling agent is commercially available 0-(7-azabenzotrizol-1-yl)-N, N,N', N'-tetramethyluronium hexafluorophosphate.
The coupling reaction is conducted in an inert solvent, e. g. dichloromethane, acetonitrile or dimethylformamide. An excess of a tertiary amine, e. g. diisopropylethylamine, N-methylmorpholine, N-methylpyrrolidine or4~DMAP is added to maintain the reaction mixture at a pH of about 8. The reaction temperature usually ranges between 0 °C and 50 °C and the reaction time usually ranges between 15 min and 24 h.
The functional groups of the constituent amino acids generally must be protected during the coupling reactions to avoid formation of undesired bonds. The protecting groups that can be used are listed in Greene, "Protective Groups in Organic Chemistry", John Wiley & Sons, New York (1981) and "The Peptides: Analysis, Synthesis, Biology", Vol. 3, Academic Press, New York (1981), hereafter referred to simply as Greene, the disclosures of which are hereby incorporated by reference.
The a-carboxyl group of the C-terminal residue is usually protected as an ester that can be cleaved to give the carboxylic acid. Protecting groups that can be used include 1) alkyl esters such as methyl, trimethylsilyl and t.butyl, 2) aralkyl esters such as benzyl and substituted benzyl, or 3) esters that can be cleaved by mild base or mild reductive means such as trichloroethyl and phenacyl esters.
The a-amino group of each amino acid to be coupled Is typically protected. Any protecting group known in the art can be used. Examples of such groups include: 1) acyl groups such as formyl, trifluoroacetyl, phthalyl, and p-toluenesulfonyl; 2) aromatic carbamate groups such as benzyloxycarbonyi (Cbz or Z) and substituted bensyloxycarbonyls, and 9-fluorenylmethyloxycarbonyI (Fmoc); 3) aliphatic carbamate groups such as tertbutyloxycarbonyl (Boc), ethoxycarbonyl, diisopropylmethoxycarbonyl, and allyloxycarbonyl; 4) cyclic alkyl carbamate groups such as cyclopentyloxycarbonyl and adamantyloxycarbonyl; 5) alkyl groups such as triphenylmethyl and benzyl; 6) trialkylsilyl such as trimethylsilyl; and 7) thiol containing groups such asphenylthiocarbonyl anddithiasuccinoyl. The preferred a-amino protecting group is either Boc or Fmoc. Many amino acid derivatives suitably protected for peptide synthesis are commercially available.
The a-amino protecting group is cleaved prior to the next coupling step. When the Boc group is used, the methods of choice are trifluoroacetic acid, neat or in dichloromethane, or HCI in dioxane or in ethyl acetate. The resulting ammonium salt is then neutralized either prior to the coupling or in situ with basic solutions such as aqueous buffers, or tertiary amines in dichloromethane or acetonitrile or dimethylformamide. When the Fmoc group is used, the reagents of choice are piperidine or substituted piperidine in dimethylformamide, but any secondary amine can be used. The deprotection is carried out at a temperature between 0 °C and room temperature usually 20-22 °C.
Any of the natural or non-natural amino acids having side chain functionalities will
typically be protected during the preparation of the peptide using any of the above described groups. Those skilled in the art will appreciate that the selection and use of appropriate protecting groups for these side chain functionalities depend upon the amino acid and presence of other protecting groups in the peptide. In the selection of such protecting groups it is desirable that the group is not removed during the deprotection and coupling of the a-amino group.
For example, when Boc is used as the a-amino protecting group, the following side chain protecting groups are suitable: p-toluenesulfonyl (tosyl) moieties can be used to protect the amino side chain of amino acids such as Lys and Arg; acetamidomethyl, benzyl (Bn), or tert-butylsulfonyl moities can be used to protect the sulfide containing side chain of cysteine; benzyl (Bn) ethers can be used to protect the hydroxy containing side chains of serine, threonine or hydroxyproline; and benzyl esters can be used to protect the carboxy containing side chains of aspartic acid and glutamic acid.
When Fmoc is chosen for the a-amine protection, usually tert. butyl based protecting groups are acceptable. For instance, Boc can be used for lysine and arginine, tert.butyl ether for serine, threonine and hydroxyproline, and tert-butyl ester for aspartic acid and glutamic acid. Triphenylmethyl (Trityl) moiety can be used to protect the sulfide containing side chain of cysteine.
/
Once the inhibitor sequence is completed any protecting groups are removed in whatever manner is dictated by the choice of protecting groups. These procedures are well known to those skilled in the art.
In compounds of Formula I, the P2 unit comprises a nitrogen-containing ring residue which is substituted with the W and R8 moieties.
Synthesis of heterocyclic P2 building blocks
The R8 group can be coupled to the P2 scaffold at any convenient stage of the synthesis of compounds according to the present invention. One approach is to first couple the R8 group to the P2 scaffold and subsequently add the other desired building blocks, i.e. P1 and optionally P3 and P4. Another approach is to couple the P1 and, if present P3 and P4 using an unsubstituted P2 scaffold and add the R8 group afterwards.
Compounds wherein W is 0 and R8 is alkyl, Co-Caalkylcarbocycylyl, Co-Csalkylheterocycylyl can be prepared according to the procedure described by E. M. Smith et a I. (J. Med. Chem. (1988), 31, 875-885), as depicted in Scheme 1, which illustrates the technique in a moiety wherein q and k are 1.
(Figure Removed)
Scheme 1
Commercially available Boc-4-(R)-hydroxyproline, or any suitable hydroxy substituted proline analogue, such as an hydroxypiperidoic acid is treated with a base such as sodium hydride or potassium t.butoxide in a solvent like dimethylformamide and the resulting alkoxide is reacted with an alkylating agent, R8-X, wherein X is a suitable leaving group such as a halide.mesylate, triflate or tosylate or the like, providing the desired substituted proline derivative.
Alternatively, when W is O or S and R8 is carbocyclyl such as phenyl or heterocyclylyl such as heteroaryl, the P2 building blocks can also be prepared via a Mitsunobu reaction (Mitsunobu, 1981, Synthesis, January, 1-28; Rano etal., Tetrahedron Lett., 1995, 36, 22, 3779-3792; Krchnaket al., Tetrahedron Lett, 1995, 36, 5, 6193-6196; Richter et al., Tetrahedron Lett., 1994, 35, 27,4705-4706) as shown in Scheme 2, which illustrates the technique in a moiety wherein q and k are 1 .

(Figure Removed)
Scheme 2
Treatment of the appropriate hydroxy substituted proline analogue, such as a hydroxypiperidoic acid, here shown as commercially available Boc-4-hydroxyproline methyl ester, with the desired alcohol or thiol (R8-WH) in the presence of triphenylphosphine and an activating agent like diethyl azodicarboxylate (DEAD), diisopropy! azodicarboxylate (DIAD) or the like, provides the ester compound (2b). Hydrolysation of the ester to the acid by standard procedures provides the P2 building block (2c).
Alcohol (2a) can alternatively be treated with phosgene thus providing the corresponding chloroformate which upon reaction with an amine, R8NHz, in the presence of a base like sodium hydrogen carbonate or triethylamine, provides carbamates i.e. W is -OC(=0)NH-, whereas reaction of alcohol (2a) with an acylating agent, R8-CO-X, like an acid anhydride or acid halide for instance the acid chloride, to provide esters, i.e. W is -OC(=O)-.
Various alcohols R8-OH, and alkylatlng agents R8-X are described in WO 00/09543 and WOOO/59929. An example of the synthesis wherein R8 is a substituted quinoline derivative is shown in Scheme 3.
(Figure Removed)
Friedel-Craft acylation of a suitable substituted aniline (3a), available either commercially or in the literature, using an acylating agent like acetyl chloride or the like in the presence of boron trichloride and aluminium trichloride in a solvent like dichloromethane provides (3b). Coupling of (3b) to a heterocyclic carboxylic acid (3c) under basic conditions, such as in pyridine, in the presence of an activating agent for the carboxylate group, for instance POCIa, followed by ring closure and dehydration under basic conditions like potassium tert-butoxide in tert-butanol provides quinoline derivative (3e). Quinoline derivative (3e) can be coupled in a Mitsunobu reaction to an alcohol as described above, or the hydroxy group can be displaced by a suitable leaving group such as a halide like chloride, bromide or iodide, by treatment of quinoline (3e) with an appropriate halogenating agent for example phosphoryl chloride or the like.
A variety of carboxylic acids with the general structure (3c) can be used in Scheme 3. These acids are available either commercially or in the literature. An example of the preparation of 2-(substituted)-amino-carboxy-aminothiazole derivatives, following the procedure by Berdikhina et a). Chem. Heterocycl. Compd. (Engl. Transl.) (1991), 427-433, is shown below.
(Figure Removed)
Thiourea (4c) with different alkyl substituents R' can be formed by reaction of the appropriate amine (4a) with tert-butylisothiocyanate in the presence of a base like diisopropylethylamine in a solvent like dichloromethane followed by removal of the tert- butyl group under acidic conditions. Subsequent condensation of thiourea derivative (4c) with 3-bromopyruvic acid provides the acid (4d).
P2 building blocks wherein the R8 substituent is attached via an amine, amide, urea or sulphonamide, can be prepared from aminoproline analogues achieved either from a suitable commercially available aminoproline, etc derivative or by transforming the hydroxy group of the corresponding hydroxy derivative into an azide group for example by transforming the hydroxy group into a suitable leaving group such as a mesylate or halogen like chloride, followed by substitution of the leaving group with azide or by the use of an azide transfer agent like diphenylphosphoryl azide (DPPA). Reduction of the azide by catalytic hydrogenation or any other suitable reduction method provides the amine. The amino derivative can be reacted in a displacement reaction with an alkylating agent of the general formula R8-X wherein R8 and X are as described for scheme 1, to form P2 building blocks for use in the preparation of compounds of general formula I, wherein W is -NH-. Reaction of the aminoproline analogue with an acid of the general formula R8-COOH under standard amide coupling conditions provides compounds wherein the R8 substituent is linked via an amide bond, whereas reaction of the aminoproline analogue with an appropriate derivative of sulphonic acid, R8-S(O)2-X where X is a leaving group for example chloride, in the presence of a base, provides sulphonamides. Compounds wherein the linkage between the cyclic scaffold and the R8 substituent is constituted of a urea group can for example be achieved by treatment of amino proline analogue with
phosgene to afford the corresponding chlorocarbamate followed by reaction with the desired amine. Alternatively, the amino proline analogue can be reacted with the carbamoyl chloride or isocyanate of the desired R8 substituent for the formation of the urea linkage. It will be apparent that corresponding reactions will be available for P2 groups with other ring sizes and substitution pattern.
4-Substituted heterocyclyl derivatives such as 4-substituted proline for use as P2 building blocks where W is -CHa- can be prepared as shown in Scheme 5, which illustrates the technique on a moiety where q and k is 1, according to the procedures described by J. Ezquerra et al., Tetrahedron, 1993, 38, 8665-8678 and C. Pedregal et al. Tetrahedron Lett., 1994, 35, 2053-2056.
(Figure Removed)
Treatment of suitably acid protected pyrrolidone or piperidinone such as commercially available Boc-pyroglutamic acid (5a) with a strong base such as lithium diisopropylamide in a solvent like tetrahydrofuran followed by addition of an alkylating agent R8-CH2-X where X is a suitable leaving group such as a halide like chloride or bromide, followed by reduction of the amide and deprotection of the ester gives the desired compound (5d).
Compounds of the present invention wherein a heterocyclic R8 group is attached directly to the cyclic P2 scaffold, i.e. W is a bond in general formula I, can be prepared for example by using a replacement reaction wherein a suitable leaving group on the P2 scaffold is replaced by the desired R8 group such as a heterocyclic group.
Alternatively the R8 group can be introduced by way of a Mitsunobu reaction wherein the hydroxy group of the P2 scaffold is reacted with a nitrogen atom in the heterocyclic R8 group.
(Figure Removed)
Compounds wherein a tetrazole derivative is attached through a carbon atom of the heterocyclic ring are conveniently prepared by building up the tetrazole moiety directly on the P2 precursor. This can be achieved for instance by transforming the hydroxy group of the P2 precursor into a cyano group followed by reaction with an azide reagent like sodium azide. Triazole derivatives can also be built up directly on the P2 precursor for example by transforming the hydroxy group of the P2 precursor into an azide group followed by a 3+2 cycloaddition reaction of the afforded azide and a suitable alkyne derivative.
Structurally diverse tetrazoles for use in the above described substitution or Mitsunobu reactions can be prepared by reacting commercially available nitrile compounds with sodium azide. Triazole derivatives can be prepared by reaction of an alkyne compound and trimethylsilyl azide. Useful alkyne compounds are available either commercially or they can be prepared for instance according to the Sonogashira reaction i.e. reaction of a primary alkyne, an aryl halide and triethylamine in the presence of PdCla(PPh)3 and Cul as described for example in A. Elangovan, Y.-H. Wang, T.-l. Ho, Org. Lett., 2003, 5,1841-1844. The heterocyclic substituent can also be modified when attached to the P2 building block either before or after coupling of the P2 building block to the other building blocks.
These methods and further alternatives for the preparation of compounds wherein W is a bond and R8 is an optionally substituted heterocycle are extensively described in
Compounds with alternative ring size and/or position of the W-R8 substituent of the proline derivatives In scheme 1, 2 and 5 may also be used in the preparation of compounds according to the present invention. For example, alkylation of commercially available 3-hydroxyproline provides compounds of the general formula (I) wherein k is Q and q is 2. Correspondingly, alkylation of 5-hydroxyproline, prepared for example as described by Hallberg et al., J. Med. Chem. (1999), 4524-4537, provides compounds of the general formula (I) wherein k is 2 and q is 0.
Various methods for the preparation of hydroxylated 2-piperidine carboxylic acids are described in the literature se for instance Celestini et al., Org. Lett., (2002), 1367-1370, Hoarau et al., Tetrahedron: Asymmetry, (1996), 2585-2594, Zhu etal., Tetrahedron Lett., 41, (2000), 7033-7036. For example, the corresponding pyridine carboxylic acids can be reduced to provide hydroxylated 2-piperidine carboxylic acids. Enzymatical methods can also be used for the preparation of hydroxylated proline analogues. For example, a 3-hydroxy substituent can be introduced on commercially available 4, 5, and 6 membered heterocyclic acids by the use of proline 3-hydroxylase as described by Ozaki et al., Tet Letters, 40, (1999), 5227-5230.
Synthesis and introduction of P1 building blocks.
The amino acids used in the preparation of P1 fragments are available either commercially or in the literature, see for example WO 00/09543 and WOOO/59929 from Boehringer-lngelheim or US2004/0048802 from BMS.
Scheme 6 shows an example of the preparation of a sulphonamide derivative to be used as a P1 fragment, and the subsequent coupling to a Boc protected P2 building block.
(Figure Removed)
The sulphonamide group can be introduced on a suitably protected amino acid (6a) by treatment of the amino acid with a coupling agent, for example N,N'-carbonyldiimidazole (GDI) or the like, in a solvent like THF followed by reaction with the desired sulphonamide (6b) in the presence of a strong base such as 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU). Alternatively the amino acid can be treated with the desired sulphonamide (6b) in the presence of a base like diisopropyl ethylamine followed by treatment with a coupling agent like PyBOP® to effect the introduction of the sulphonamide group. Removal of the amino protecting group by standard methods and subsequent coupling to a P2 building block, prepared as described above, using standard methods for amide bond formation, like with a coupling agent as O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU) in the presence of a base such as diisopropylamine in a solvent like dimethylformamide, gives Boc protected P2-P1 compound (6e). Alternatively, the sulphonamide group can be introduced at a later stage of the synthesis, for example as the last step. In this case an amino acid with the reversed protection pattern, i.e. having an unprotected amino function and a protected acid function, is coupled to the acid function of the P2 building block using standard peptide coupling conditions for example as described above. Removal of the acid
protection group, using the appropriate conditions for the protection group used, followed by coupling of the sulphonamide as described above, yields compound 6e
P1 building blacks for the preparation of compounds according to general formula I wherein A is an ester or an amide can be prepared by reacting amino acid (6a) with the appropriate amine or alcohol respectively under standard conditions for amide or ester formation. Compounds according to general formula I wherein A is CR4R4' can be prepared by coupling of the appropriate P1 building block to the P2 building block as described in Oscarsson et al Bioorg Med Chem 2003 11(13) 2955-2963 and PCT/EP03/10595 filed 23.09.2 O03, the contents of which are incorporated by reference.
Compounds comprising an azapeptide P1 residue, i.e. Q is NRu in general formula I can be prepared by using a suitable P1 aza-amino acyl moiety in the coupling to the P2 fragment. The preparation of aza-amino acyl moieties is described by M. D. Bailey et al. in J. Med. Chem., 47, (2O04), 3788-3799, and an example is shown in scheme 6A.
\(Figure Removed)

Incorporation of the appropriate N-linked side chain, Ru, on commercially available tert-butylhydrazine can be performed for example by a reductive amination reaction with the appropriate aldehyde or ketone as described in scheme 19 below which
produces the N-alkylated carbazate (6Aa). Condensation of 6Aa with a desired chloroformate in the presence of a base like triethylamine or diisopropylethylamine in a solvent like THF provides 6Ab. The R11 moiety can then optionally be removed using the appropriate conditions depending on the specific R1', such as catalytic hydrogenation for R11 being benzyl, which gives the corresponding acids. Subsequent reaction of the afforded acid with a desired sulphonamide derivative as described in scheme 6 yields sulphonamide capped building blocks. Alternatively, reaction of carbazate 6Aa with an isocyanate, R3-N=C=O, provides building blocks for the preparation of compounds according to general formula I, wherein M is NRu and A is CONHR3.
The P2 and P3 moieties may be linked together prior to or after the introduction of the P1 building block.
Synthesis of capped P3 and P3-P4 building blocks
The building blocks R16-G-P3 and R16-G~P4-P3 can be prepared as generally
depicted in scheme 7.
(Figure Removed)
A suitable N-protected amino acid (7a) can be coupled with an amino capping group (R16-NHRy) using standard peptide coupling conditions like with coupling agents such as HATU, DCC, HOBt or the like in the presence of a base such as DIEA or DMAP in a solvent like dichloromethane, chloroform or dimethylformamide or a mixture thereof and ester formation conditions like providing amides i.e. G is NHRy (7b). Alternatively, reaction of amino acid (7a) with a compound of general formula R16-X where R16 is as defined above and X is a leaving group such as a halide, in the presence of a base like cesium carbonate or silver (I) oxide provides esters, i.e. G is O (7b). On the other hand, amino acid (7a) can be coupled to a second, suitably O-protected, amino acid (7d) using standard peptide coupling conditions as described above, providing (7e). Displacement of the ester group with a suitable capping group (7b) provides fragment (7f) useful for the preparation of compounds according to the present invention wherein m and n are 1.
When G is N-Ry, the capped P3 or P2 building block can also be prepared on solid support as exemplified in Scheme 8.

(Figure Removed)
An appropriate N-protected, for example Boc protected, amino acid (8a) can be immobilized on a solid support, here exemplified by Agronaut resin PS-TFP, by reacting the amino acid with the desired solid support in the presence of coupling
reagent like N.N'-diisopropylcarbodiimide and a base like DMAP in a solvent like dichloromethane and dimethylformamide. The immobilized amino acid can then be cleaved from the support with a suitable capping group (8c) thus giving fragments useful for the preparation of compounds according to the present invention wherein m or n is 1, Optionally the amino protecting group can be removed followed by coupling of an appropriate amino acid using standard methods thus providing fragments useful for the preparation of compounds according to the present invention wherein m and n are 1.
Coupling of a capping group or a capped building block to the P2-P1 construct The R16-G, R1e-G-P3 or R16-G-P4-P3 building block linked via a urea functionality to the P2-P1 construct, can be introduced as depicted in scheme 9, which illustrates the technique with a variant in which the P2 scaffold is a 5-membered ring.

(Figure Removed)
A chlorocarbamate group can be formed onto the ring amine of the P2-P1 construct (9a) by removal of the amine protection group by standard procedures, like acidic treatment with for example TFA in dichloromethane or the like when the Boc group is
used, followed by reaction of the free amine with phosgene in toluene in the presence of a base such as sodium hydrogen carbonate or triethylamine in a solvent like tetrahydrofuran. Subsequent reaction of the formed electrophilic center with the amino group of a R1B-NH2, R16-NH-NH2, R16-G-P3 or R16-G-P4-P3 building block (9c) in a solvent like dlchloromethane in the presence of a base like sodium hydrogen carbonate provides (9d). Compounds of general formula (I) wherein E is C=S, S(=0) . or S(=0)2 can be prepared according to the above procedure but with the use of reagents like thiocarbonyl diimidazole, thionyl chloride or sulphuryl chloride respectively instead of phosgene.
Compounds containing a hydrazine group linked to the P2 unit, i.e. X is -NRjNRj- in general formula I, or when the P3 and P4 units are absent and G is NRjNRj, can be prepared as depicted below. Scheme 10 shows the introduction of a hydrazine derivative to a 5-membered P2 building block.
(Figure Removed)
Reaction of tert-butyl carbazate (10a), optionally alkyl substituted on one or both nitrgogens, with p-nitrophenyl chloroformate in the presence of a base like sodium hydrogen carbonat followed by addition of the P2 building block (10b) provides the urea derivative 10c. The phosgene method described in scheme 9 can alternatively be used to effect the linkage of the fragments 10a and 10b. Optional removal of the boc group by standard procedures like acidic treatment with for example TFA in a
suitable solvent such as dichloromethane, provides the hydrazine containing derivative (10d). Alternatively, any appropriate hydrazine derivative, such as morpholin-1-ylamine, piperidin-1-ylamine or the like can be linked to 9Ab instead of the tert-butyl carbazate derivative.
The achieved compound can then be further extended by cuopling of a P3 or P4-P3 building block to the primary amine of compound 9Ad for example as shown in scheme 11.
(Figure Removed)
Treatment of the a-amino compound (11a) with sodium nitrite, potassium bromide and sulphuric acid (Yang et at. J, Org. Chem. (2001), 66, 7303-7312) provides the corresponding a-bromo compound (1 1b) which upon reaction with the above described derivative (10d) provides the hydrazine containing derivative (11 c).
The linkage between the P2 and P3 building blocks may also be constituted of a carbamate group and a general route to such compounds is depicted in Scheme 12, which illustrates the technique with a variant in which P2 is a proline derivative.

(Figure Removed)
The desired, optionally protected, amino capping group (12a) is coupled to a hydroxy acid (10b) using standard peptide coupling techniques followed by reaction with the electrophilic P2 building block (12d) described above and optional deprotection provides construct (12e).
Compounds lacking a carboxy group in the P3 unit can be prepared as illustrated in Scheme 13, which illustrates the technique as applied to a compound of Formula I
(Figure Removed)
Chlorocarbamoyl derivative (13a) can be reacted in a displacement reaction with an azide derivative (13b), prepared by methods known from the literature, in the presence of a base like sodium hydrogen carbonate to give (13c). X is as described for general formula (I). Reduction of the azide function for example by polymer bound triphenyl phosphine in a solvent like methanol or any other suitable reduction method provides intermediate (13d) which subsequently can be reacted with an acid under peptide coupling conditions or with an amlne in a reductive amination reaction providing amides and secondary amines respectively.
Scheme 14 shows an alternative route towards compounds lacking a carboxy group in the P3 unit.
(Figure Removed)
Instead of using the azide derivative (13b) in scheme 13 the corresponding, optionally protected, hydroxy derivative (14b) can be used in the displacement reaction with the chlorocarbamate (14a) and thus introducing a primary alcohol. The alcohol (14c) can then, after optional deprotection, be oxidized with a suitable oxidizing agent like for example Dess-Martin periodinane to form the corresponding aldehyde. Reaction of the aldehyde with a desired amin^ in a reductive amination reaction using a reagent like for example polystyrene bound cyanoborohydride in a solvent like THF provides amine derivatives (14e).
Alternatively alcohol (14c) can be reacted with a suitable acylating or alkylating agent under the appropriate conditions to provide ester and ether compounds respectively, i.e. G is O in general formula (I).
Subsequent reaction of the formed alcohol with a suitable acylating or alkylating agent using the appropriate conditions provides the ester and ether compounds respectively, i.e. G is O in general formula (I).
Alternatively the linkage between the P2 and P3 building blocks can be via a guanidine group and a general route to such compounds is depicted in Scheme 15.
(Figure Removed)
Treatment of the P2-building block (1 5a) with thlocarbonyl dlimidazoie or the like in a solvent like dimethylformamide followed by condensation with sodium cyanamide in a solvent like ethanol affords the thiolate intermediate (15b). Reaction of intermediate (15b) with the desired building block, here shown as a capped P3 building block (12c) provides the cyanoguanidine derivative (15d). Other building blocks, R16-G or R16-G-P4-P3, can alernatively be coupled to the intermediate (15b). Hydrolysis of the cyano group by treatment of (15d) with diluted hydrochloric acid gives the guanylurea derivative (15e).
When R7, R7' and A' contains functional groups, these are suitably protected by methods recognized by persons skilled in the art, see for example Bodanzky or Greene cited above.
Formation of macrocvclic compounds
Compounds according to the present invention wherein an aikylene chain extending from the Rr/R7' cycloalkyl to Rx or R11 thus forming a macrocycle, can be prepared as described below. Suitable P1, P2 and P3 building blocks, or precursors thereof, are coupled together using the strategies described above, followed by a ring-closing reaction (macrocyclization). The substituent W-R8 of the P2 building block can be incorporated via a Mitsunobu reaction as described above, before or after formation of the macrocycle or the desired building blocks can be coupled together using the appropriately substituted P2-building block. For macrocyclic structures extending from the Rr/R7 cycloalkyl to R11, P3 amino acids containing the appropriate side chain can be prepared as described in WOOO/59929.
A typical route to macrocyclic compounds is shown in Scheme 18 which illustrates the technique applied to a compound having a 5-membered P2 scaffold and a spiro-cyclopropyl group in the P1 moiety, where the macrocycle extends from the P3 side chain.
(Figure Removed)
Coupling of proline derivative (16a) with the appropriate, acid protected, amino acid (16b) using e.g. the phosgene conditions described above provides (16c). Formation of the macrocycle can then be carried out via an olefin metathesis reaction using a Ru-based catalyst such as the one reported by Miller, S.J., Blackwell, H.E.; Grubbs, R.H. J. Am. Chem. Soc, 118, (1996), 9606-9614, Kingsbury, J. S., Harrity, J. P. A., Bonitatebus, P. J., Hoveyda, A. H., J. Am. Chem. Soc. 121, (1999), 791-799 and Huang et al., J. Am. Chem. Soc, 121, (1999), 2674-2678. It will also be recognized that catalysts containing other transition metals such as Mo can be used for this reaction. Optionally the double bond is reduced and/or the ethyl ester is hydrolysed by standard hydrogenation and/or hydrolysation methods respectively well known in the art. Alternatively the methyl ester can be selectively hydrolysed followed by coupling of a R16-G-P4 building block by standard peptide coupling conditions. The macrocyclisation step described in Scheme 16 can also be applied to the corresponding carbocyclic analogues described above. When the linker contains a
nitrogen atom the ring closure can be carried out by reductive aminatlon as described in WOOO/59929.
Macrocyclic compounds without the cyclopropyl moiety in the P1 part, i.e. the macrocyclic ring extends directly from the peptidic backbone at the carbon adjacent R7, can be prepared using the methods described herein. An example wherein a proline derivative is used as the cyclic P2 scaffold is shown in scheme 17.
(Figure Removed)
Coupling of a suitable allylglycine derivative (17a), to the acid function of the P2 building block (17b) using standard peptide coupling conditions yields the amide derivative (17c). Removal of the Boc protection group by acidic treatment followed by formation of a chlorocarbamate by treatment with phosgene in the presence of
odium hydrogencarbonate and subsequent reaction with the olefin substituted amino acid (17d) provides the urea compound (17e). A ring closing metathesis reaction is then effected by using for example Hoveyda-Grubbs catalyst which gives the macrocyclic compound (17f).
Even though scheme 17 shows the synthetic sequence using a P2 building block wherein the R8 substituent is attached to the scaffold, it will be apparent that an unsubstituted P2 scaffold could be used and the R8 group introduced at any suitable stage of the synthesis, using any of the methods described herein.
Building blocks to be used in the preparation of compounds wherein the macrocycle extends from the nitrogen in the linkage between the P2 and P3 fragments i.e. X is NRx in general formula I, or in the preparation of compounds wherein the P3 and P4 fragments are absent, i.e. m and n are 0 and G is NRj in general formula I, can typically be prepared as outlined in scheme 18B.
(Figure Removed)
Carbamate 18a, which is commercially available or Is readily prepared for instance by reaction of the desired alkyl amine with di-tert-butyl dicarbonate, can be reacted with an appropriate w-unsaturated alcohol under Mitsunobu conditions to provide the alkylated carbamate (18b). Subjection of 18b to acidic conditions like for example treatment with trifluoroacetic acid in a solvent like dichloromethane gives the free amine (18c) which can be linked to a P2 fragment using any of the previously described strategies.
Macrocyclic structures containing a hydrazine group i.e. X is NRjNRj or m and n are 0 and G is NRjNRj, in general formula I, can be prepared by linking a suitably N-
alkylated carbazate derivative to the P2 fragment. Alkylated carbazate derivatives can be prepared, for example, as described in Scheme 19.
(Figure Removed)
Oxidation of the appropriate alcohol (19a) effected by a suitable oxidation method like for example with N-methyl morpholine oxide and tetrapropylammonium perruthenate in a solvent like dlchloromethane provides aldehyde (19b). Reductive alkylation of tert-butyl carbazate with the afforded aldehyde gives the desired N-alkylated building block (19c). Alternatively, any desired hydrazine derivative such as morpholin-1-ylamine, piperidin-1-ylamine or the like can be used instead of tert-butyl carbazate in the reaction with aldehyde 19b.
Scheme 20 illustrates synthetic sequences to building blocks suitable for the preparation of compounds wherein the "outer" nitrogen of the hydrazine group is alkylated, either with an to-unsaturated alkyl chain appropriate for subsequent macrocycle formation or with any other suitable alkyl group.
(Figure Removed)

Reaction of a suitably protected hydrazine derivative, for example (1,3-dioxo-1,3-dihydro-isonidol-2-yl)-carbannic acid tert-butyl ester (20a), which can easily be prepared by a person skilled in the art, with a desired alcohol, R-OH, under Mitsunobu conditions provides N-alkylated hydrazine compound (20b). Removal of the phtalimido group effected by treatment with hydrazine or a derivative thereof like hydrazine hydrate or hydrazine acetate provides the carbazate (20c). The afforded primary amine can then either be be coupled to any desired P2 fragment using any of the methods previously described to give the urea derivative (20d) or alternatively it can be further alkylated using for example the reductive amination method described in scheme 19 followed by coupling to a P2 fragment as previously described to give 20e.
Scheme 21 exemplifies the coupling of a hydrazine containing P3 building block to a cyclopentane scaffold followed by macrocyclisation.
(Figure Removed)
Coupling of the carbazate derivative (21 b) to the P2-P1 building block (21 a) using standard peptide coupling conditions provides intermediate (21 c). Ring closure of (21 c) by an olefin metathesis reaction as described in scheme 18 gives the macrocyclic compound (21d).
The term "N-protecting group" or "N-protected" as used herein refers to those groups intended to protect the N-terminus of an amino acid or peptide or to protect an amino group against undesirable reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in Greene, "Protective Groups in Organic Synthesis" (John Wiley & Sons, New York, 1981), which is hereby incorporated by reference. N-protecting groups include acyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoracetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, oc-chlorobutyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and the like; sulfonyl groups such as benzenesulfonyl, p-toluenesulfonyl, and the like, carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl,
3,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyIoxycarbonyl, 1 -(p-biphenylyl)-l -methylethoxycarbonyl, a,a-dimethyl-3,5-dimethoxybenzyloxycarbonyl, benzhydryloxycarbonyl, t-butoxycarbonyl, diisopropylmethoxycarbonyl, Isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2-trichloroethoxycarbonyl, phenbxycarbonyl, 4-nitrophenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl, and the like; alkyl gropus such as benzyl, triphenylmethyl, benzyloxymethyl and the like; and silyl groups such as trimethylsilyl and the like. Favoured N-protecting groups include formyl, acetyl, benzoyl, pivaloyl, t-butylacetyl, phenylsulfonyl, benzyl, t-butoxycarbonyl (BOG) and benzyloxycarbonyl (Cbz).
Hydroxy protecting group as used herein refers to a substituent which protects hydroxyl groups against undesirable reactions during synthetic procedures such as those O-protecting groups disclosed in Greene, "Protective Groups In Organic Synthesis," (John Wiley & Sons, New York (1981)). Hydroxy protecting groups comprise substituted methyl ethers, for example, methoxymethyl, benzyloxymethyl, 2-methoxyethoxymethyl, 2-(trimethylsilyl)ethoxymethyl, t-butyl and other lower alkyl ethers, such as isopropyl, ethyl and especially methyl, benzyl and triphenylmethyl; tetrahydropyranyl ethers; substituted ethyl ethers, for example, 2,2,2-trichloroethyl; silyl ethers, for example, trimethylsilyl, t-butyldimethylsilyl and t-butyldiphenylsilyl; and esters prepared by reacting the hydroxyl group with a carboxylic acid, for example, acetate, propionate, benzoate and the like.
In treating conditions caused byflavivirus such as HCV, the compounds of formula I are typically administered in an amount to achieve a plasma level of around 100 to 5000 nM, such as 300 to 2000 nM. This corresponds to a dosage rate, depending on the bioavailability of the formulation, of the order 0.01 to 10 mg/kg/day, preferably 0.1 to 2 mg/kg/day. A typical dosage rate for a normal adult will be around 0.05 to 5 g per
day, preferably 0.1 to 2 g such as 500-750 mg, in one to four dosage units per day. As with all Pharmaceuticals, dosage rates will vary with the size and metabolic condition of the patient as well as the severity of the infection and may need to be adjusted for concomitant medications.
As is good prescribing practice with antiviral therapy, the compounds of formula I are typically coadministered with other HCV therapies to avoid the generation of drug escape mutants. Examples of such additional HCV antiviral therapies include ribavirin, interferons, including pegylated interferons. Additionally a number of nucleoside analogues and protease inhibitors are in clinical or preclinical development and will be amenable to co-administration with the compounds of the invention.
Accordingly a further aspect of the invention provides a composition comprising a compound of formula I and at least one further HCV antiviral in a common dosage unit, such as any of the dosage forms described below, but especially an orally administered tablet, or capsule or a liquid suspension or solution for oral or injection use. A further aspect of the invention provides a method for the treatment or prophylaxis of flavivirus infection, such as HCV, comprising the sequential or simultaneous administration of a compound of formula I and at least one further HCV antiviral. A related aspect of the invention provides a patient pack comprising a first pharmaceutical composition, preferably in unit dosage form, of the compound of formula I and a second pharmaceutical composition, typically also in unit dosage form and generally in a separate container within the patient pack, of a second HCV antiviral. A patient pack will conveniently also be provided with instructions printed on the package or a container therein, or on a package insert, for the simultaneous or sequential administration of the respective pharmaceutical compositions.
Many HCV patients are co-infected, or prone to superinfection, with other infectious diseases. Accordingly, a further aspect of the invention provides combination therapies comprising the compound of the invention co-formulated in the same
dosage unit or co-packaged with at least one further anti-infective pharmaceutical. The compound of the invention and the at least one further antinfective are administered simultaneously or sequentially, typically at doses corresponding to the monotherapy dose for the agent concerned. However, certain antifectives can induce a synergistic response, allowing one or both of the active ingredients to be administered at a lower dose that the corresponding monotherapy. For example in drugs prone to rapid metabolism by Cyp3A4, co-dosing with the HIV protease inhibitor ritonavir can allow lower dosage regimes to be administered.
Typical coinfections or superinfections with HCV include hepatitis B virus or HIV. Accordingly the compound of the invention is advantageously co-administered (either in the same dosage unit, co-packaged or separately prescibed dosage unit) with at least one HIV antiviral and/or at least one HBV antiviral.
Representative HIV antivirals include NRTI such as alovudine (FLT), zudovudine (AZT, ZDV), stavudine (d4T, Zerit), zalcitabine (ddC), didanosine (ddl, Videx), abacavir, (ABC, Ziagen), lamivudine (3TC, Epivir), emtricitabine (FTC, Emtriva), racevir (racemic FTC), adefovir (ADV), entacavir (BMS 200475), alovudine (FLT), tenofovir disoproxil fumarate (TIMF, Viread), amdoxavir (DAPD), D-d4FC (DPC-817), -dOTC (Shire SPD754), elvucitabine (Achillion ACH-126443), BCH 10681 (Shire) SPD-756, racivir, D-FDOC, GS7340, INK-20 (thioether phospholipid AZT, Kucera), 2'3'-dideoxy-3'-fluoroguanosine (FLG) & its prodrgus such as M1V-210, reverset (RVT, D-D4FC, Pharmasset DPC-817).
Representative NNRTI include delavirdine (Rescriptor), efavirenz (DMP-266, Sustiva), nevirapine (BIRG-587, Viramune), (n-)calanolide A and B (Advanced Life Sciences), capravirine (AG1549f S-1153; Pfizer), GW-695634 (GW-8248; GSK), MIV-150 (Medivir), MV026048 (R-1495; Medivir AB/Roche), NV-05 2 2 (Idenix Pharm.), R-278474 (Johnson & Johnson), RS-1588 (Idenix Pharm.), TMC-120/125 (Johnson & Johnson), TMC-125 (R-165335; Johnson & Johnson), UC-781 (Biosyn Inc.) and YM215389 (Yamanoushi).
Representative HIV protease inhibitors include PA-457 (Panacos), KPC-2 (Kucera Pharm.), 5 HGW-43 (Enzo Biochem), amprenavir (VX-478, Agenerase), atazanavir (Reyataz), indinavir sulfate (MK-639, Crixivan), Lexiva (fosamprenavir calcium, GW -433908 or 908, VX-175), ritonavir (Norvir), lopinavir + ritonavir (ABT-378, Kaletra), tipranavir, nelfinavir mesylate (Viracept), saquinavir (Invirase, Fortovase), AG1776 (JE-2147, KNI-764; Nippon Mining Holdings), AG-1859 (Pfizer), DPC-681/684 (BMS), GS224338; Gilead Sciences), KNI-272 (Nippon Mining Holdings), Nar-DG-35 (Narhex), P(PL)-100 (P-1946; Procyon Biopharma), P-1946 (Procyon Biopharma), R-944 (Hoffmann-LaRoche), RO-0334649 (Hoffmann-LaRoche), TMC-114 (Johnson & Johnson), VX-385 (GW640385; GSKA/ertex), VX-478 (Vertex/GSK).
Other HIV antivirals include entry inhibitors, including fusion inhibitors, inhibitors of the CD4 receptor, inhibitors of the CCR5 co-receptor and inhibitors of the CXCR4 coreceptor, or a pharmaceutically acceptable salt or prodrug thereof. Examples of entry inhibitors are AMD-070 (AMD11070; AnorMed), BlockAide/CR (ADVENTRX Pharm.), BMS 806 (BMS-378806; BMS), Enfurvirtide (T-20, R698, Fuzeon), KRH1636 (Kureha Pharmaceuticals), ONO-4128 (GW-873140, AK-602, E-913; ONO Pharmaceuticals), Pro-140 (Progenies Pharm), PR0542 (Progenies Pharm.), SCH-D (SCH-417690; Schering-Plough), T-1249 (R724; Roche/Trimeris), TAK-220 (Takeda Chem. Ind.), TNX-355 (Tanox) and UK-427,857 (Pfizer). Examples of integrase inhibitors are L-870810 (Merck & Co.), c-2507 (Merck & Co.) and S(RSC)-1838 (shionogi/GSK).
Examples of HBV antivirals include adefovir dipivoxil (Hepsera), and especially lamivudine and 2'3'-dideoxy-3'-fluoroguanosine (FLG) & its prodrugs such as MIV-210, the S'-O-valyl-L-lactyl prodrug of FLG. These latter HBV antivirals are particularly convenient as they are also active against HIV.
While it is possible for the active agent to be administered alone, it is preferable to present it as part of a pharmaceutical formulation. Such a formulation will comprise the above defined active agent together with one or more acceptable carriers or excipients and optionally other therapeutic ingredients. The carrier(s) must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient.
The formulations include those suitable for rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration, but preferably the formulation is an orally administered formulation. The formulations may conveniently be presented In unit dosage form, e.g. tablets and sustained release capsules, and may be prepared by any methods well known in the art of pharmacy.
Such methods include the step of bringing into association the above defined active agent with the carrier. In general, the formulations are prepared by uniformly and intimately bringing into association the active agent with liquid carriers or finely divided solid carriers or both, and then if necessary shaping the product. The invention extends to methods for preparing a pharmaceutical composition comprising bringing a compound of Formula I or its pharmaceutically acceptable salt in conjunction or association with a pharmaceutically acceptable carrier or vehicle. If the manufacture of pharmaceutical formulations involves intimate mixing of pharmaceutical excipients and the active ingredient in salt form, then it is often preferred to use excipients which are non-basic in nature, i.e. either acidic or neutral. Formulations for oral administration in the present invention may be presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active agent; as a powder or granules; as a solution or a suspension of the active agent in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water in oil liquid emulsion and as a bolus etc.
With regard to compositions for oral administration (e.g. tablets and capsules), the term suitable carrier includes vehicles such as common excipients e.g. binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, polyvinylpyrrolidone (Povidone), methylcellulose, ethylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, sucrose and starch; fillers and carriers, for example corn starch, gelatin, lactose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride and alginic acid; and lubricants such as magnesium stearate, sodium stearate and other metallic stearates, stearic acid, glycerol stearate, silicone fluid, talc waxes, oils and colloidal silica. Flavouring agents such as peppermint, oil of wintergreen, cherry flavouring or the like can also be used. It may be desirable to add a colouring agent to make the dosage form readily identifiable. Tablets may also be coated by methods well known in the art.
A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active agent in a free flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface-active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may be optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active agent.
Other formulations suitable for oral administration include lozenges comprising the active agent in a flavoured base, usually sucrose and acacia or tragacanth; pastilles comprising the active agent in an inert base such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active agent in a suitable liquid carrier,
The compounds of formula I can form salts which form an additional aspect of the invention. Appropriate pharmaceutically acceptable salts of the compounds of formula I include salts of organic acids, especially carboxylic acids, including but not limited to acetate, trifluoroacetate, lactate, gluconate, citrate, tartrate, maleate, malate,
pantothenate, isethionate, adipate, alglnate, aspartate, benzoate, butyrate, digluconate, cyclopentanate, glucoheptanate, glycerophosphate, oxalate, heptanoate, hexanoate, fumarate, nicotinate, palmoate, pectinate, 3-phenylpropionate, picrate, pivalate, proprionate, tartrate, lactobionate, pivolate, camphorate, undecanoate and succinate, organic sulphonic acids such as methanesulphonate, ethanesulphonate, 2-hydroxyethane sulphonate, camphorsulphonate, 2-napthalenesulphonate, benzenesulphonate, p-chlorobenzenesulphonate and p-toluenesulphonate; and inorganic acids such as hydrochloride, hydrobromide, hydroiodide, sulphate, bisulphate, hemisulphate, thiocyanate, persulphate, phosphoric and sulphonic acids. The invention further extends to salts of the compounds of formula I which may or may not be pharmaceutically acceptable, but which are useful as synthetic intermediates, the salt moiety being displaced or replaced as necessary.
The invention includes prodrugs of the compounds of formula I. Prodrugs of the compounds of formula I are those compounds which following administration to a patient release a compound of the formula I in vivo generally following hydrolysis in the gut, liver or plasma. Typical prodrugs are pharmaceutically acceptable ethers and especially esters (including phosphate esters) of hydroxy functions, pharmaceutically acceptable amides or carbamates of amine functions or pharmaceutically acceptable esters of carboxy functions. Preferred pharmaceutically acceptable esters include alkyl esters, including acetyl, ethanoyl, butyryl, t-butyryl, stearyl and pivaloyl, phosphate esters and sulphonic esters (ie those derived from RSC^OH, where R is lower alkyl or aryl). Pharmaceutically acceptable esters include lower alkyl ethers and the ethers disclosed in WOOD/47561, especially methoxyaminoacyl and ethoxyaminoacyl.
The compounds of the invention have various steric centres and the invention extends to racemates and enantiomers at each of these steric centres.
Typically, the stereochemistry of the groups corresponding to the P3 and P4 side chains (ie R15 and/or R11) will correspond to an L-amino acid configuration, although

the invention also extends to D-isomers at one or both of these centres. It is noteworthy that the L configuration is active notwithstanding that the nature of the E moiety means that P3 and P4 are typically translated one atom relative to a conventional polypeptide and the fact that the reversal of a peptide residue, as envisaged for P3 and P4 then pitches the amine acid side chain to the opposite side compared to a conventional peptide substrate.
The stereochemistry of the backbone component of the cyclic P2 group (i.e. spanning the carbonyl of the P1 amide bond and the carbonyl or E extending of P3 will typically correspond to L-proline. The stereochemistry of the P2 ring atom to which W is bonded is typically as shown:
(Figure Removed)
In compounds of the invention wherein R7 and Rr together define a spiroaikyi group, such a spiro-cycloalkyl will typically comprise an R7a substltuent on the spiro-cyclopropyl ring which is is orientated syn to A:
(Figure Removed)
Conveniently, the spiro carbon of such a spiro-cyclopropyl ring has the R configuration:
(Figure Removed)
Conveniently an R7'a substituent on a spiro-cyclopropyl ring adjacent to A is in a syn orientation in the following absolute configuration:
(Figure Removed)
Particularly preferred variants have R7a include ethyl, hence the asymmetric carbon atoms at position 1 and 2 have the R, R configuration. Alternative preferred R7a include vinyl, hence the asymmetric carbon atoms at position 1 and 2 have the R, S configuration.
Where the compound of the invention is a macrocycle comprising a J group, J is preferably a diastereomer represented by partial structures (i) or (ii):
(Figure Removed)
Detailed Description of the Embodiments
Various embodiments of the invention will now be described by way of illustration only
with reference to the following non-limiting examples.
Example 1

(Figure Removed)
7-Methoxy-2-phenyl-quinolin-4-ol (1 ).
To a stirred round bottled flask with toluene (100 ml) ethyl benzoyl acetate (18.7 g, 97 mmol) and m-anisidine (12 g, 97 mmol) was added. 4 M HCI in dioxane (0.5 mL) was added and the reaction mixture was refluxed for 6 h (140 °C). The mixture was co-evaporated with toluene. To the crude mixture diphenyl ether (50 ml) was added and the mixture was heated to 280 °C for 2 h. When the theoretical amount ethanol (6 mL) was collected in a Dean Stark trap the heating was stopped and the mixture was cooled to rt. The crude mixture was dissolved in CHaCfe (100 ml) and stirred for 30 min. The formed precipitate was filtered off and dried which gave 1 (4.12 g, 16.4 mmol, 17 %): pale yellow powder.
1H (300 MHz, dmso-db): 8 3.8 (s, 3H), 6.24 (s, 1H), 6.88-6.96 (dd, 1H, J = 9.07 Hz, J = 2.47 Hz), 7.19 (d, 1H, J=2.19 Hz), 7.56 (t, 3H, J = 2.19 Hz), 7.8 (dd, 2H, J- 7.14 Hz, J = 2.19 Hz), 8.0 (d, 1H, J = 9.06 Hz); 13C (75.5 MHz, DMSO-D6): 8 55.3, 99.6, 106.9,113.1, 119.1,126.4, 127.5, 128.8, 130.2,134.1, 142.2, 149.4, 161.8,176.4.
Example 2
(Figure Removed)
Boc-L-fe/f-leucine-OH (2).
Triethylamine (890 ^L, 6.40 mmol) was added dropwise to a stirred solution of L-tenf-leucine (300 mg, 2.29 mmol) and dke/f-butyl dicarbonate (599 mg, 2.74 mmol) in dioxane/ water 1:1 (8 ml) and the solution was stirred overnight. The mixture was extracted with petroleum ether (2x) and the aqueous phase was cooled to 0 °C and carefully acidified to pH 3 by slow addition of 4M NaHSO4-H2O. The acidified water phase was extracted with EtOAc (3x) and the combined organic phases were washed with brine (2x) and was then dried, filtered and concentrated to give the title
compound (522 mg, 99 %) as a colorless powder. No further purification was
needed.
1H-NMR (300 MHz, CD3OD) 8 0.99 (s, 9H), 1.44 (s, 9H), 3.96 (s, 1H); 13C-NMR (75.5
MHz, CD3OD) 8 27.1, 28.7, 34.9, 68.0, 80.5, 157.8,174.7.
Example 3
(Figure Removed)
((S)-Cyclohexyl-methylcarbamoyl-methyl)-carbamic acid ferf-butyl ester (3). Boc-Chg-OH (387 mg, 1.50 mmol) was coupled to methylamine hydrochloride (111 mg, 1.65 mmol) using the same HATU coupling conditions as in the synthesis of compound 7. The crude product was extracted with EtOAc, washed with brine and concentrated. Purification by flash column chromatography (EtOAc) provided the title compound (307 mg, 76 %) as a colorless solid.
1H-NMR (300 MHz, CDCI3) 8 0.91-1.13 (m, 2H), 1.14-1.31 (m, 3H), 1.44 (s, 9H), 1.61-1.80 (m, 6H), 2.80 (d, J = 4.7 Hz, 3H), 3.91 (dd, J - 7.1, 9.1 Hz, 1 H), 5.23 (b, 1H), 6.52 (bs, 1H); 13C-NMR (75.5 MHz, CDCI3) 8 25.9, 26.0, 26.1, 28.3, 28.5, 29.6, 40.5, 59.5,79.7,155.9,172.4.
Example 4
(Figure Removed)
{(S)-1-[((S)-Cyclohexyl-methylcarbamoyl-methyl)-carbamoyl]-2,2-dimethyl-propyl}-carbamic acid fe/f-butyl ester (4)
To a solution of compound 3 (98 mg, 0.362 mmol) in methylene chloride (3 ml) were added triethylsilane (115 ml, 0.742 mmol) and TFA (3 ml). The mixture was stirred for 2 h at room temperature and was then evaporated and coevaporated with toluene.
The deprotected amine was dissolved in DMF (5 mL) and coupled to compound 2 (84 mg, 0.363 mmol) using the same HATU coupling conditions as in the synthesis of 7. The crude product was extracted with EtOAc, washed with brine, dried, filtered and concentrated. Purification by flash column chromatography (toluene/ EtOAc 1:1) provided the title compound (128 mg, 92 %) as a colorless solid. 1H-NMR (300 MHz, CDCI3) 5 0.99 (s, 9H), 1.02-1.30 (m, 5H), 1.44 (s, 9H), 1.58-1.77 (m, 4H), 1.78-1.89 (m, 2H), 2.79 (d, J = 4.7 Hz, 3H), 4.11 (d, J = 9.3 Hz, 1H), 4.33 (app. t, J= 8.5 Hz, 1H), 5.65 (b, 1H), 7.25 (b, 1H), 7.39 (b, 1H); 13C-NMR (75.5 MHz, CDCI3) 8 25.9, 25.9, 26.0, 26.2, 26.8, 28.4, 29.0, 29.7, 34.5, 39.7, 58.4, 62.4, 79.4, 156.0,171.4,171.8.
Example 5
Hept-6-enal (5)
To a solution of hept-6-en-1-ol (1 mL, 7.44 mmol) and N-methylmorpholine N-oxide (1.308 g, 1 1 .17 mmol) in DCM (17 ml) was added ground molecular sieves (3.5 g, 4 A). The mixture was stirred for 10 min at room temperature under nitrogen atmosphere before tetrapropylammonium perruthenate (TRAP) (131 mg, 0.37 mmol) was added. After stirring for additional 2.5 h the solution was filtered through celite. The solvent was then carefully evaporated and the remaining liquid was purified by flash column chromatography (DCM) to give the volatile aldehyde 5 (620 mg, 74%) as an oil.
Example 6
(Figure Removed)
Ay-Hept-6-en-(£)-yIidene-hydrazinecarboxylic acid te/t-butyl ester (6) To a solution of 5 (68 mg, 0.610 mmol) and ferf-buty! carbazate (81 mg, 0.613 mmol) in MeOH (5 ml) was added ground molecular sieves (115 mg, 3A). The mixture was
stirred for 3 h after which it was filtered through celite and evaporated. The residue was dissolved in dry THF (3 mL) and AcOH (3mL). NaBH3CN (95 mg, 1,51 mmol) was added and the solution was stirred over night. The reaction mixture was diluted with saturated NaHCOs solution (6 mL) and EtOAc (6 mL). The organic phase was washed with brine, saturated NaHCO3, brine, dried over MgSO4 and evaporated. The cyanoborane adduct was hydrolyzed by treatment with MeOH (3 mL) and 2 M NaOH. (1.9 mL). The mixture was stirred for 2 h and the MeOH was evaporated. HzO (5 mL) and DCM (5 mL) were added and the water phase was extracted three times with DCM. The combined organic phases were dried and evaporated. Purification by flash column chromatography (toluene/ethyl acetate 9:1 with 1 % triethylamine and toluene/ethyl acetate 6:1 with 1 % triethylamine) provided the title compound (85 mg, 61 %) as an oil.
Example 7
(Figure Removed)
((S)-1-CycIopentylcarbamoyl-2,2-dimethyl-propyl)-carbamic acid fe/f-butyl ester (7). To a cold solution of 2 (133 mg, 0.575 mrnol), cyclopentylamlne (64 jj,L, 0.648 mmol) and DIEA (301 \iL, 1.73 mmol) in DMF (3 mL) was added the coupling reagent HATU (240 mg, 0.631 mmol). The mixture was stirred for half an hour and for additional two hours at room temperature. The solvent was removed by heating the reaction flask in a water bath under diminished pressure and the residue was dissolved in ethyl acetate, after which the organic phase was washed three times with brine, dried, filtered and evaporated. Purification by flash column chromatography (toluene/ethyl acetate 4:1) provided the title compond (140 mg, 82 %) as colorless crystals.
1H-NMR (300 MHz, CDCI3): 5 0.95 (s, 9H), 1.28-1.48 (m, overlapped, 2H), 1.40 (s, 9H), 1.49-1.71 (m, 4H), 1.86-2.01 (m, 2H), 3.76 (b, 1H), 4.09-4.23 (rn, 1H), 5.32 (b,
1H), 5.91 (b, 1H); 13C-NMR (75.5 MHz, CDCI3): 5 23.6, 23.7, 26.5, 28.3, 32.6, 33.1, 34.5, 51.0,62.2, 79.4, 155.9, 170.3.
Examples
(Figure Removed)
(S)-ferf-Butoxycarbonylamino-cyclohexyl-acetic acid methyl ester (8) To a solution of Boc-Chg-OH (53 mg, 0.206 mmol) in acetone (3 ml) were added methyl iodide (195 jaL, 3.1 mmol) and silver (I) oxide (53 rng, 0.229 mmol). The mixture was allowed to stir over night in a reaction flask that was covered with aluminium foil. Thereafter the solution was filtered through celite and evaporated. Purification by flash column chromatography (toluene/ethyl acetate 15:1) provided methyl ester 8 (56 mg, 100 %) as a colorless oil.
1H-NMR (300 MHz, CDCI3): 8 1.00-1.34 (m, 5H), 1,44 (s, 9H), 1.54-1.82 (m, 6H), 3.73 (s, 3H), 4.20 (dd, J = 2.8, 5.0 Hz, 1H), 5.05 (bs, 1H); 13C-NMR (75.5 MHz, CDCI3): 5 26.0, 28.2, 28.3, 29.5, 41.1, 52.0, 58.3, 79.7,155.6, 172.9.
Example 9
(Figure Removed)
(S)-((S)-2-Benzyloxycarbonylamino-3-methyl-butyrylamino)-cyclohexyl-aceticacid methyl ester (9)
Compound 8 (93 mg, 0.343 mmol) was deprotected and coupled to Z-Val-OH (95 mg, 0.378 mmol) according to the method for the preparation of 39. Flash column chromatography (toluene/ethyl acetate 4:1) gave the title compound (131 mg, 94 %) as a colorless solid.
1H-NMR (300 MHZ, CDCI3): 5 0.92-1.30 (m, 11H), 1.54-1.88 (m, 6H), 2.02-2.18 (m, 1H), 3,72 (s, 3H), 4.05-4.18 (m, 1H), 4.52 (dd, J= 3.0, 5.5 Hz, 1H), 5.12 (a, 2H), 5.49 (bs, 1H), 6.52 (bs, 1H), 7.34 (s, 5H); 13C-NMR (75.5 MHz, CDCI3): 5 17.8,19.0, 25.8, 28.2, 29.3, 31.2, 40.5, 51.9, 56.8, 6O.O, 66.8,127.7, 127.9, 128.1, 128.3, 136.2, 156.3,171.3,172.2.
Example 10
(Figure Removed)
N-Boc-4R-(2-phenyl-7-methoxyquinoilne-4-oxo)proline (10). To a stirred solution of N-Boc-trans-4-hydroxy-L-proline (3.9 g, 16.9 mmol) in DMSO (90mL) was added potassium tertbutoxide (4.5 g, 40.1 mmol). After 1 hrs 4-chloro-2-phenyI-7-methoxy quinoline (4.5g, 16.7 mmol) was added and stirred at RT for 12 hrs. The mixture was diluted with water (180 ml), washed with ethyl acetate (1x30mL) and neutralized with 1N HCI. The solid was filtered, washed with water and dried giving (4.65g, 10mmol) of product. >95% purity by HPLC. M+H+ 464.2.
Example 11
(Figure Removed)
2-(1-Ethoxycarbonyl-2-vinyl-cyclopropylcarbamoyl)-4-(7-methoxy-2-phenyl-quinoline-4-yloxy)~pyrrolidine-1 -carboxylic acid tenf.butyl ester (11).
To a solution of 1-amino-2-vinyl-cyclopropanecarboxylic acid ethyl ester (41 mg, 0.26 mmol), 10 (11 mg, 0.22 rnmol), HATU (204 mg, 0.54 mmol) in DMF (4 ml) was added diisopropyehtylamine (187 \il, 1.08 mmol). After stirring at RT for 1 hrs, dichloromethane (4 ml) was added. The solution was washed with aqueous NaHC03 (sat) and with two portions of water. The organic layer was dried and concentrated. The product was pure enough (>95 % by HPLC) to be used in the next step. M+H* 602.2.
Example 12
(Figure Removed)
1-{[4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid ethyl ester (12).
Compound 11 was kept in TFA-DCM 1:2 (3 mL) at RT for 60 min. Toluene (3 ml)
was added. The sample was co-evaporated to dryness. Purity by HPLC >95%. M+H+
502.4.
Example 13
(Figure Removed)
1-{[1-[1-(2-Hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyI]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (13).
To a solution of compound 12 (0.13 mmol) in THF (2 mL), was added a large excess of NaHCO3 (s) and a solution of phosgene in toluene (1.6 M, 600 pL). After 10 min of agitation the slurry was filtered and concentrated to dryness. The solid was redissolved in dichloromethane and a large excess of NaHCOa (s) and 2-Amino-A/-(2-hydroxy-indan-1-yl)-3,3-dimethyl-butyramide (0.65 mmol) was added. The slurry was agitated for 24-40 hrs at RT. The slurry was filtered, concentrated and subjected to silica column chromatography (gradient elution from 100 % DCM to MeOH/DCM 2:98) to give the title compound (89.6 mg, 0.11 mmol). Purity by HPLC >95%. M+H+ 790.3.
Example 14
(Figure Removed)
1-[1-[1-(2-Hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyl-propyl]-4-(6-methoxy-3-phenyl-naphthalen-1-yloxy)-pyrroIidin-2-yl]-2-vinyl-cyclopropanecarboxylicacid (14). To a solution of 13 (76.7mg, 0.097mmol) in THF-MeOH 2:3 (2 ml) was added 1M LiOH 5 equiv. The solution was kept at 60 °C for 60 min. After cooling to RT, HOAc 15-30 eq. was added followed by toluene (2 mL) and then concentrated to dryness. The residue was taken up in DCM and washed with water. The organic layer was dried and concentrated to give the title compound (72 mg, 0.094 mmol). Purity >95% by HPLC M+H* 762.2.

(Figure Removed)
A/-(2-Hydroxy-indan~1 -yl)-2-[4-(6-methoxy-3-phenyl-naphthalen-1 -yloxy)-2-(1 -phenylmethanesulfonylaminocarbonyl-2-vinyl-cyclopropyl)-pyrroIidin-1-yl]-3,3-dirnethyl-butyramide (15).
To solution of 14 (25 mg, 0.033 mmol) in chloroform (1 mL) was added benzenesulfonamfde (10.5 mg, 0.066 mmol) followed by dilsopropylethylamine (34 nL, 0.197mmol). The solution was stirred at RT for 10 min and then at -20 °C for 30 min. PyBOP (76 mg, 0.13 mmol) was then added as a solid. The solution was kept at -20 °C for 48 hours. The solution was then poured into aqueous NaHCOa (sat.) and washed with water. The organic layer was dried, concentrated and subjected to purification by HPLC, affording the title compound as a white solid.
Example 16
(Figure Removed)
Resin bound 2-tert.butoxycarbonylamino-3,3-dimetylbutyric acid (16). To Argonaut resin PS-TFP (1.38 mmol/g, 10 g) and 2-fe/f-butoxycarbonylamino-3,3-dimethyl-butyric acid (4.5 g, 20.7mmol) was added dichloromethane (40 ml) and DMF (10 mL). To this mixture was added DMAP (1 g, 8.28 mmol) and then DIG (9.5 ml, 60.7 mmol). After 3 hrs of agitation at RT the resin was filtered and washed successively with DMF, THF, DCM, THF, DCM and ether and then dried in a vacuum.
Example 17
(Figure Removed)
[1-(2-Hydroxy-indan-1-ylcarbamoyl)-2,2-dlmethyl-propyl]-carbamic acid tert.butyl ester
To a portion of 16 (200 mg) in DCM aminoindanol (0.14 mmol) was added. The mixture was agitated for 2 hrs. The liquid was filtered of and the resin washed with 2xDCM. The combined liquids were combined and concentrated to dryness to afford the title compound (20.5 mg, 0.055 mmol) Purity >95% by HPLC. M+H* 363.15. 13C NMR 8C(100 MHz; CDCI3; Me4Si) 27.0, 28.5, 34.2, 39. 8, 50.8, 57.9, 68.2, 73.7, 124.8, 125:6, 127.4,128.5,140.4, 171.6.1H NMR 8H(400 MHz; CDCI3; Me4Si) 1.07 (9H, s, CCH3), 1.44 (9H, s, OCCH3), 2.93 (1H, dd, Jgem16.4 Hz, J3,2 2.3 Hz, CH2), 3.15 (iH, dd, Jaem16.4 Hz, J3,2 5.2 Hz, CH2),
Example 18
(Figure Removed)
2-Amino-A/-(2-hydroxy-indan-1-yl)-3,3-dimethyl butyramide (18).
Compound 17 was kept in DCM-TFA 2:1 (2 mL) for 60 min at RT. The solution was
co-evaporated with toluene to dryness.
Example 19
(Figure Removed)
(2-ferf-Butoxycarbonylamino-3,3-dimethyl-butyrylamino)-cyclohexyl-aceticacid methyl ester (19).
To a solution of 2~ferf.butoxycarbonylamino-3,3-dimethyl butyric acid (500 mg, 2.16 mmol), Amino-cyclohexyl-acetic acid methyl ester (444 mg, 2.59 rnmol) and HATU (2 g, 5.40 mmol) in DMF (20 ml) was added diisopropylethylamine (1.88 ml, 10.8 mmol). The solution was stirred for 1 hrs at r.t. and diluted with dichloromethane (40 ml). This solution was washed with aqueous. NaHC03 (sat.) and water (x2), dried and concentrated. The product was >95 % pure. M+H* 385.4.
Example 20
(Figure Removed)
{1-[(Cyclohexyl-methylcarbamoyI-methyl)-carbamoyl]-2,2-dirnethyl-propyl}-carbamic acid fe/t-butyl ester (20).
To compound 19 in EtOH-THF 1:2 was added a large excess of methylamine (30% in water) and left at rt. for 2 weeks. The solution was concentrated to dryness and the residue subjected to a short silica gel column eluted with 2% MeOH in dichloromethane to give a pure (>95%) product M+H* 384.5.
Example 21
(Figure Removed)
2-Amino-N-(cyclohexyl-methylcarbamoyl-methyl)-3,3-dimethyl-butyramide (71), Compound 20 was kept In dichloromethane-trifuoroacetic acid 2:1 for 1 h at rt and concentrated to dryness. The residue was dried in a vacuum for 16 hrs. Reversed phase C18 HPLC showed >95% purity M+hf 283.1.

Example 22
(Figure Removed)
(1 R,2S)-1 -{[(2S ,4R)-1 -((1 S,2R)-2-Hydroxy-indan-1-ylcarbamoyl)-4-(7-methoxy-2-
phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid (22).
Compound 12 was treated as described for the preparation of 13 but with the use of
(1S,2R)-cis-1-amino-2-indanol instead of 2-amino-N-(2~hydroxyindan-1-yl)-3,3-
dimethyl butyramide followed by ester hydrolysis as described for the preparation of
compound 14 which gave the title compound. Purity by HPLC >95%. M+H* 649.1.
Example 23
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-(Cyclohexylmethyl-carbamoyl)-2-methyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl~cyclopropanecarboxylic acid (23).
N-(tert-butoxycarbonyl)-L-valine was attached to the resin as described for the preparation of compound 16 followed by reaction with cyclohexylamine as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%.lvHH*712.3.
Example 24
(Figure Removed)
(1RF2S)-1-{t(2S,4R)-1-((1R)-2-Hydroxy-1-phenyl-ethyicarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyllc acid (24).
Compound 12 was treated as described for the preparation of 13 but with the use of (R)-2-phenylglycinol instead of 2-amino-N-(2-hydroxyindan-1-yl)-3,3-dimethyl butyramide instead of 2-amino-A/-(2-hydroxy-indan-1-yl)-3l3-dimethyl-butyramide followed by ester hydrolysis as described for the preparation of compound 14 which gave the title compound. Purity by HPLC >95%. M+H* 637.1.
Example 25 '
(Figure Removed)
(1 R,2S)-1-{[(2S,4R)-1-{[(1 S)-Cyclohexyl-(cyclohexylmethyl-carbamoyl)-methyl]-carbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yIoxy)-pyrrolidine-2-carbonyI]-amino}-2-vinyl-cyclopropanecarboxylic acid (25).
N-(tert-butoxycarbonyl)-L-cyclohexylglycine was attached to the resin as described for the preparation of compound 16 followed by reaction with cyclohexanemethylamine as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%. M+H+ 752.4.
Example 26
(Figure Removed)
(1 R,2S)-1 -{[(2S,4R)-1-[(1 S)-2-CyclohexyI-1 -(cyclohexylmethyl-carbamoyl)-ethylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (26).
N-(tert-butoxycarbonyi)-L-cyclohexyialanine was attached to the resin as described for the preparation of compound 16 followed by reaction with
cyclohexanemethylamine as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%. M+H* 766.4.
Example 27
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-(Cyclohexylmethyl-carbamoyl)-2l2-dimethyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (27).
N-(tert-butoxycarbonyl)-L-tert-butyglycine was attached to the resin as described for the preparation of compound 16 followed by reaction with cyclohexanemethylamine as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%. M+H* 726.3.
Example 28
(Figure Removed)
(1 R,2S)-1 -{[(2S,4R)-1 -[(1 S)-1 -(Cyclohexylmethyl-carbamoyl)-2-phenyl-ethylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cycIopropanecarboxylic acid (28).
N-(tert-butoxycarbonyl)-L-phenylalanine was attached to the resin as described for the preparation of compound 16 followed by reaction with cyclohexanemethylamine as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 1 2 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%. M+H+ 760.4.
Example 29
(Figure Removed)
(1 R,2S)-1 -{[(2S,4R)-1 -[(1 S)-1 -((1 S,2R)-2-Hydroxy~indan-1 -ylcarbamoyl)-3-phenyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-viny!~cyclopropanecarboxylic acid (29).
N-(tert.butoxycarbonyl)-L-phenethylglycine was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S,2R)~cis~1-amino-2-indanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%. Mn-K1" 810.4.
Example 30
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-((1S)-1-Benzylcarbamoyl-2-methyl-propylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (30).
N-(tert-butoxycarbonyl)-L-valine was attached to the resin as described for the preparation of compound 16 followed by reaction with benzylamine as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%. M+H* 706.2.
Example 31
(Figure Removed)
(1Rl2S)-1-{[(2S,4R)-1-[(1S)-1-((1R)-2-Hydroxy-1-phenyl-ethylcarbamoyi)-2,2-dimethyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (31). N-(tert-butoxycarbonyl)-L-tert-butygIycine was attached to the resin as described for the preparation of compound 16 followed by reaction with (R)-2-phenylglycinol as described for the preparation of 17 and removal of the Boc group as described for 18.

The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which gave the title compound. Purity by HPLC >95%.M+H* 750.3.
Example 32
(Figure Removed)
(1R, 2S)-1-{[(2S, 4R)-1-[(1 S)-1 -((1 R)-lndan-1-ylcarbarnoyl)-2-methyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrroiidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyiic acid (32).
(2S)-ferf-butoxycarbonylamino-3-methylbutyric acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1 R)-1-aminoindane as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (12.5 mg, 28 % yield), Purity by HPLC >90%. M+H+ 732.2.
Example 33
(Figure Removed)
propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyIic acid (33).
(2S)-fe/f-butoxycarbonylamino-3~methylbutyric acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S)-1-aminoindane as described for the preparation of 17 and removal of the Boo group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (22 mg, 49 % yield), Purity by HPLC >90% M+H* 732.2.
Example 34
(Figure Removed)
, 4R)-1-[(1S)-1-(2-hydroxyethylcarbamoyl)-2-methyl-propylcarbamoyI]-4-(7-methbxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (34).
(2S)-te/f-butoxycarbonylamino-3-methylbutyric acid was attached to the resin as described for the preparation of compound 16 followed by reaction with 2-aminoethanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 1 3 which, after purification by HPLC, gave the title compound (3 mg, 8 % yield), Purity by HPLC >90% M+H* 660.2.
Example 35
(Figure Removed)

, 2R)-2-Hydroxy-indan-1-ylcarbamoy!)-2-methyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yIoxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cycIopropanecarboxylic acid (35).
(2S)-terf-butoxycarbonylamino-3-methylbutyrlc acid was attached to the resin as
described for the preparation of compound 16 followed by reaction with (1S,2R)-1-
amino-2-indanol as described for the preparation of 17 and removal of the Boc group
as described for 18. The afforded compound was then reacted with the
chlorocarbamate achieved from 12 as described for the preparation of 13 which, after
purification by HPLC, gave the title compound (10 mg, 22 % yield), Purity by HPLC
>90% M+H+ 748.2.
Example 36
(Figure Removed)
, 2S)-2-Hydroxy-indan-1-ylcarbamoyl)-2-methyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyIic acid (36).
(2S)-ferf-butoxycarbonyIamino-3-rnethylbutyric acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1R,2S)-1-amino-2-indanol as described for the preparation of 17 and removal of the Boc group
as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (11 mg, 24 % yield), Purity by HPLC >75% M+H+ 748.
Example 37
(Figure Removed)

5,4R)-1-{[Cyclohexyl-(S)-((1 S, 2R)-2-hydroxy-indan-1-ylcarbamoyl)-methyl]-carbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (37).
(2S)-te/tbutoxycarbonylamino-cyclohexylacetic acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S,2R)-1-amino-2-indanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (7.5 mg, 16 % yield), Purity by HPLC >95% M+H4 788.3.
Example 38
(Figure Removed)
, 2R)-2-Hydroxy-indan-1-ylcarbamoyl)-2.2-dimethyl-propylcarbamoyl]-4-(7-methoxy-2-phenyI-quinol!n-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (38). (2S)-ferf-butoxycarbonylamino-3,3-dimethylbutyric acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S,2R)-1-amlno-2-indanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (12 mg, 26 % yield), Purity by HPLC >95% M+H+ 762.3.
Example 39
(Figure Removed)
, 2S)-H[(2S, 4R)-1-[(1S)-1-((1S, 2R)-2-Hydroxy-indan-1-ylcarbamoyl)-3,3-dimethyl-butylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (39). (2S)-te/f-butoxycarbonylamino-4,4-dimethylpentanoic acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S,2R)-1-amino-2-indanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (14.2 mg, 30 % yield), Purity by HPLC >95% M+H+ 776.3.
Example 40
(Figure Removed)
2R)-2-Hydroxy-indan-1-ylcarbamoyl)-2-phenyl-etylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolldine-2-carbonyl]-amino}-2-vinyI-cycIopropanecarboxylic acid (40).
(2S)-terf-butoxycarbonylamino-3-phenylpropanoic acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S,2R)-1-amino-2-indanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (2.4 mg, 5 % yield), Purity by HPLC >95% M+H+ 796.2.
Example 41
(Figure Removed)
4R)-1-[(1S)-2-Cyclohexyl-1-((1S, 2R)-2-hydroxy-indan-1-ylcarbamoyl)-ethylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyc!opropanecarboxylic acid (41).
(2S)-terf-Butoxycarbonylamino-3-cyclohexylpropanoic acid was attached to the resin as described for the preparation of compound 16 followed by reaction with (1S,2R)-1-amino-2-indanol as described for the preparation of 17 and removal of the Boc group as described for 18. The afforded compound was then reacted with the
chlorocarbamate achieved from 12 as described for the preparation of 13 which, after purification by HPLC, gave the title compound (12.3 mg, 25 % yield), Purity by HPLC >95% M-t-hT 802.3.
Example 42
(Figure Removed)

(1R, 2S)-1 -{[(2S, 4R)-H(1 S)-1 -[(S)-(Cyc!ohexyl-methylcarbamoyl-methyl)-carbamoyl]-2,2-dimethyl-propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (42).
Compound 12 was treated as described for the preparation of 13 but with the use of 21 instead of 2-amino-A/-(2-hydroxy-indan-1-yI)-3,3-dimethyl-butyramide followed by ester hydrolysis as described for the preparation of compound 14 which, after purification by HPLC, gave the title compound(8.6 mg, 18 % yield). Purity by HPLC >95%. M+H+ 783.3.
Example 43
(Figure Removed)
1 -(2-Amino-4-methoxyphenyl)ethanone (43)
/n-Anisidine (10.0 g, 82 mmol) was dissolved in CHaCfe (50 mL), and the solution was cooled to -50 °C. BCI3 (1 M in CH2CI2) 82 mL, 82 mmol) was added slowly during 20 min, after which the mixture was stirred at -50 °C for 30 min, followed by sequential addition of AcCI (6.0 mL, 84 mmol) and AICI3 (11 g, 82 mmol). The mixture was
stirred at -50 °C for 1 h and was then allowed to assume it After stirring at rt overnight, the solution was heated at 40 °C for 4 h, after which the mixture was poured over ice. The aqueous mixture was made alkaline with 10 % NaOH (w/v) and extracted with EtOAc (4 x 200 ml). The combined organic phases were washed with brine, dried (MgSO4), and evaporated to give a black solid, which was purified by flash column chromatography (ether/CH2CI2 20:80). The resulting solid was recrystallized from ether/hexane to give compound 93 as shiny tan leaflets (5.6 g, 42
Example 44
A/-(ferf-Butyl)-A/I-isopropylthiourea (44)
To a solution of ferf-butylisothiocyanate (5.0 ml, 39 mmol) in CH2CI2 (200 mL) were added isopropylamine (4.0 ml, 47 mmol) and diisopropylethylamine (DIEA) (6.8 ml, 39 mmol), and the mixture was stirred at rt for 2h. The reaction mixture was diluted with EtOAc, washed with 10 % citric acid (2x), saturated NaHCO3 (2x), H2O (2x), and brine (1x). The organic layer was dried (MgSCXv) and evaporated to yield compound 94 (3.3 g, 52 %) as a white solid which was used without further purification.
Example 45
/V-Isopropylthiourea (45)
Compound 44 (3.3 g, 20 mmol) was dissolved in cone. HCI (45 mL) and the solution was refluxed for 40 min. The mixture was allowed to cool to rt and then cooled in an ice bath and basified to pH 9.5 with solid and saturated NaHC03, after which the product was extracted into EtOAc (3x). The combined organic phases were washed with H2O (2x) and brine (1x), dried (MgSO4), and evaporated to yield crude compound 95 (2.1 g, 90 %) which was used without further purification.
Example 46
(Figure Removed)
2-(lsopropylamino)"-1,3-thiazole-4-carboxylic acid hydrobromide (46) A suspension of compound 45 (2.1 g, 18 mmol) and 3-bromopyruvic acid (3.0 g, 18 mmol) in dioxane (180 ml) was heated to 80°C. Upon reaching 80 °C the mixture became clear, and soon thereafter the product started to precipitate as a white solid. After 2 h of heating, the reaction mixture was cooled to rt and the precipitate was filtered off and collected. This yielded pure compound 46 (4.4 g, 94 %).
Example 47
(Figure Removed)
A/-(2-Acetyl-5-methoxyphenyl)-2-(isopropylamino)-1,3-thiazole-4-carboxamide(47) A mixture of compound 46 (4.4 g, 16.5 mmol) and the aniline derivative 93 (2.75 g, 16.5 mmol) in pyridine (140 mi) was cooled to -30 °C (upon cooling, the clear solution became partially a suspension). POCIa (3.3 mL, 35 mmol) was added slowly over a 5 min period. The mixture was stirred at -30 °C for 1 h, and was then allowed to assume rt. After stirring at rt for 1.5 h the reaction mixture was poured over ice, and the pH was adjusted to about 9-10 using solid and saturated NaHCOa. The crude product was extracted into CHeCb (3x) and the combined organic phases were dried (MgSO4) and evaporated. The crude dark-beige solid was purified by flash column chromatography (hexane/EtOAc 55:45) to give compound 47 (5.6 g, 76 %) as a pale yellow solid.
Example 48
(Figure Removed)
2-[2-(lsopropylamino)-1,3-thiazoI-4-yl]-7-methoxyquinolin-4-ol (48) A solution of t.BuOK (2.42 g, 21 mmol) in anhydrous t.BuOH (40 mL) was heated to reflux. Compound 47 (1.8 g, 5.4 mmol) was added portion-wise over a 5 min period, and the dark red solution formed was stirred at reflux for an additional 20 min. The mixture was cooled to rt, and HCI (4 M in dioxane, 8.0 ml, 32 mmol) was added, after which the reaction mixture was concentrated under vacuum. In order to assure that all of the HCI and dioxane were removed, the crude product was re-dissolved in CH2CI2 twice and thoroughly evaporated to obtain the slightly impure HCI salt of compound 98 (1.62 g) as a brown solid. The product was dissolved in CH2CI2 and washed with saturated NaHCOg, after which the aqueous phase was extracted several times with CHzClz. The combined organic phases were dried (MgSCH) and evaporated to give the title compound (1.38 g, 81 %) as a light brown solid (> 95 % pure according to HPLC tests). 1H-NMR (MeOH-d4, 400 MHz): 8 1.30 (d, J = 6.0 Hz, 6H), 3.93 (s, 3H), 3.95-4.07 (m, 1H), 6.73 (s, 1H), 6.99 (dd, J - 2.4, 9.2 Hz, 1H), 7.26 (d, J= 2,4 Hz, 1H), 7.37 (s, 1H), 8.10 (d, J = 9.2 Hz, 1H).
Example 49
(Figure Removed)

(1S)-1-{[(2S,4R)-2-(1-Methoxycarbonyl-butylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy) ]-pyrrolidine}-carboxyiic acid tert-butyl ester (49)

Reaction of 10 with Nva-OMe hydrochloride according to the method described in example 11 provided the title compound. Purity > 95% by HPLC, M+H+ 578.24.
Example 50
(Figure Removed)
(1S)-1-{[(2S,4R)-2-[4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-
amino}-pentanoic acid methyl ester (50)
Compound 49 was kept in TFA-DCM 1:2 (3 ml) at RT for 60 min. Toluene (3 mL)
was added. The sample was co-evaporated to dryness. Purity by HPLC > 95%. M+H*
478.21.
Example 51
(Figure Removed)
(1S)-2-{[(2S>4R)-1-[(1S)-1-(Cyclohexylmethyl-carbamoyl)-2-methyl-propylcarbamoyl]-
4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-pentanoic
acid methyl ester (51)
To a solution of 50 (0.1 mmol) in THF (4 ml), cooled to 0 °C, was added a large
excess of NaHCO3 (s) and a solution of phosgene in toluene (0.2 mmol, 21 jj.l). After
10 min of agitation the slurry was filtered and concentrated to dryness. The solid was
redissolved in dichloromethane and a large excess of NaHC03 (s) and 2-amino-N-cyclohexylmethyl-3-methyl-butyramide, described in example 23, (0.15 mmol) was added. The slurry was agitated 30 hrs at RT. The slurry was filtered, concentrated and subjected to silica column chromatography (gradient elution from 100 % DCM to MeOH/DCM 2:98) to give the title compound (30 mg, 0.042 mmol). Purity by HPLC > 95%. M+H+716.40.
Example 52
(Figure Removed)
(1 S)~2-{[(2S,4R)-1 -[(1 S)-1 -(Cyclohexylmethyl-carbamoyl)-2-methyl-propylcarbamoyl]-
4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyQ-amino}-pentanoic
acid (52)
To a solution of 51 (26 mg, 0.036 mmol) in THF-MeOH 2:3 (2 mL) was added 1M
LiOH 1.5 equiv. The solution was kept at 60 °C for 60 min. After cooling to RT, HOAc
was added followed by toluene (2 ml) and then concentrated to dryness to give the
title compound (25 mg, 0.035 mmol). Purity > 95% by HPLC M+H* 702.34.
Example 53
(Figure Removed)
(1R,2S)-H[(2S,4R)-1-[2-(2-Methoxy-phenoxy)-ethylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyI]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (53)
To a solution of 12 (0.06 mmol) in THF (2 ml), was added a large excess of NaHCO3 (s) and a solution of phosgene in toluene (0.078 mmol). After 10 min of agitation the slurry was filtered and concentrated to dryness. The solid was redissolved in diehloTomethane and a large excess of NaHCOs (s) and 2-(2-methoxy-phenoxy)-ethylamine (15 mg, 0.09 mmol) was added. The slurry was agitated for 30 hrs at RT. The slurry was filtered, concentrated to dryness, redissolved in MeOH and subjected HPLC purification to give the title compound (10.6 mg, 0.015 mmol). Purity by HPLC >95%. M+H+695.17.
Example 54
(Figure Removed)
(1Rt2S)-1-{I(2S,4R)-1-[2-(2-Methoxy-phenoxy)-ethylcarbamoyl]-4'(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (54)
To a solution of 53 (10.6 mg, 0.0153 mmol) in THF-MeOH 2:3 (2 mL) was added 1M LiOH 10 equiv. The solution was kept at 50 °C for 60 min. After cooling to RT, HOAc 25 equiv. was added followed by toluene (2 ml) and then concentrated to dryness. The residue was taken up In ethyl acetate, filtered and concentrated to dryness to give the title compound (9.4 mg, 0.014 mmol). Purity > 95% by HPLC M+H* 667.14.
Example 55
(Figure Removed)
(1 R,2S)-1 -{[(2S,4R)-1-((1 S,2R)-5-Hydroxy-4,5,6,7-tetrahydro-benzo[b]thiophen-4-yl-carbamoyl))-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (55)
The procedure described in example 53 was followed but with the use of 2-amino-4,5,6,7-tetrahydro-benzo[b]thiophen-5-ol instead of 2~(2-methoxy-phenoxy)-ethylamine, followed by hydrolysis of the ethyl ester as described in example 54 which gave the title compound (7.5 mg, 0.011 mmol). Purity > 95% by HPLC M+H* 669.
Example 56
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(3R)>-3-Hydroxy-pyrrolidine-1-carbonyl)]-4-(7-methoxy-2-
phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid (56)
The procedure described In example 53 was followed but with the use of (R)-3-
pyrrolidinol instead of 2-(2-methoxy-phenoxy)-ethylamine, followed by hydrolysis of
the ethyl ester as described in example 54 which gave the title compound (4 mg,
0.007 mmol). Purity > 95% by HPLC M+H+ 587.1.
Example 57
(Figure Removed)
(1 R,2S)-1 -{[(2S ,4RH-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1 -[(thiophen-2-yl-
methyl)-carbamoyl]-pyrrolidine-2-carbonyI}-amino)-2-vinyl-cyclopropanecarboxylic
acid (57)
The procedure described in example 53 was followed but with the use of thiophene-2-
methylamine instead of 2-(2-methoxy-phenoxy)-ethylamine, followed by hydrolysis of
the ethyl ester as described in example 54 which gave the title compound (8 mg,
0.013 mmol). Purity > 95% by HPLC M+H+ 613.08.
Example 58
(Figure Removed)
(1 R,2S)-H[(2S,4R)-1 [(1,1 -Dioxo-tetrahydro-1 -A,6-thiophen-3-yl -carbamoyl)-4-(7-
methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid (58)
The procedure described in example 53 was followed but with the use of 3-
aminotetrahydro-1 H-1X6-thiophene-1,1-dione instead of 2-(2-methoxy-phenoxy)-
ethylamine, followed by hydrolysis of the ethyl ester as described in example 54

which gave the title compound (13 mg, 0.02 mmol). Purity > 95% by HPLC M-H-T 635.05.
Example 59
(Figure Removed)
2-Amino-3,3-dimethyl-N-thiophen-2-yl-methyl-butyramide(59) The title compound was prepared as described in example 17 but with the use of thiophene-2-methylamine instead of aminoindanole followed by removal of the Boc group as described in example 18.
Example 60
(Figure Removed)
Z-Amino-Ne-hydroxy.S.e.T-tetrahydro-benzotbpiophen-S-yO-S.S-dimethyl-butyramide (60)
The title compound was prepared as described in example 17 but with the use of 2-amino-4,5,6,7-tetrahydro-benzo[b]thiophen-5~ol instead of aminoindanole followed by removal of the Boc group as described in example 18.
Example 61
(Figure Removed)
2-Amino-N-(2-diethylamino-ethyl)-3,3-dimethyl-butyramlde (61) The title compound was prepared as described in example 17 but with the use of N,N-diethylethylenediamine Instead of aminoindanole followed by removal of the Boc group as described in example 18.
Example 62

(Figure Removed)

2-Amino-N-[2-(2-methoxy-phenoxy)-ethyl]-3,3-dimethyl-butyramide(62) The title compound was prepared as described in example 17 but with the use of 2-methoxyphenoxyethylamine instead of aminoindanole followed by removal of the Boc group as described in example 18.
Example 63
(Figure Removed)

2-Amino-1-(3-hydroxy-pyrrolidin-1-yl)-3,3"-dimethyl-butan-1-one(63) The title compound was prepared as described in example 17 but with the use of (R)-3-pyrrolidinone instead of aminoindanole followed by removal of the Boc group as described in example 18.
Example 64
(Figure Removed)

2-Amino-N-(1,1 -dioxo-tetrahydro-1 -A,6-thiophen-3-yl)-3,3-dimethyl-butyramide (64)
The title compound was prepared as described in example 17 but with the use of 2-methoxyphenoxyethylamine instead of aminoindanole followed by removal of the Boc group as described in example 18.
Example 65
(Figure Removed)
(1R,2S)-H[(2S(4R)-1-[(1S)-1-(2,2-DimethyH-[(thiophen-2-yl-methyl)-carbamoyl]-propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (65)
To a solution of 12 (0.06 mm'ol) in THF (2 ml), was added a large excess of NaHCOa (s) and a solution of phosgene in toluene (0.078 mmoi). After 10 min of agitation the slurry was filtered and concentrated to dry ness. The solid was redissolved in dichloromethane and a large excess of NaHCOa (s) and 59 (0.09 mmoi) was added. The slurry was agitated for 30 hrs at RT. The slurry was filtered, concentrated to dryness, re-dissolved in MeOH and subjected HPLC purification to give the title compound (15.5 mg, 0.02 mmoi). Purity by HPLC > 95%. M+hT 754.2.
Example 66
(Figure Removed)

(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-(2,2-Dimethyl-1-[(thiophen-2-ylmethyl)-carbamoyl]-propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyI-cyclopropanecarboxylic acid (66)
To a solution of 65 (14 mg, 0.017 mmol) in THF-MeOH 2:3 (2 ml) was added 1M LiOH 10 equiv. The solution was kept at 50 °C for 60 min. After cooling to RT, HOAc 20 equiv. was added followed by toluene (2 ml_) and then concentrated to dryness. The residue was taken up in ethyl acetate, filtered and concentrated to dryness to give the title compound (13 mg, 0.017 mmol). Purity > 95% by HPLC M+H+ 748.13.
Example 67
(Figure Removed)
(1R,2S)-1-{[(2S,4RH1S)-1-[(1S,2R)-1-[1-(5-Hydroxy-4,5,6,7-tetrahydro-
benzo[b]thiophen-4-yl-carbamoyl)-2,2-dimethyl-propylcarbamoyl]-4-(7-methoxy-2-
phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid (67)
The procedure described in example 65 was followed but with the use of 60 instead
of 59, followed by hydrolysis of the ethyl ester as described in example 66 which gave
the title compound (4 mg, 0.005 mmol). Purity > 95% by HPLC M+H* 782.16.
Example 68
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-(2-Diethylamino-ethylcarbamoyl)-2,2-dimethyl-
propylcarbamoyl]-4*(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-
amino}-2-vinyl-cyclopropanecarboxylic acid (68)
The procedure described in example 65 was followed but with the use of 61 instead
of 59, followed by hydrolysis of the ethyl ester as described in example 66 which gave
the title compound (6 mg, 0.008 mmol). Purity > 95% by HPLC M+H+ 729.24.
Example 69
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-[2-(2-Methoxy-phenoxy)-ethylcarbamoyl]-2,2-dimethyl-
propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-
amino}~2-vinyl-cyolopropanecarboxylic acid (69)
The procedure described in example 65 was followed but with the use of 62 instead
of 59, followed by hydrolysis of the ethyl ester as described in example 66 which gave
the title compound (3 mg, 0.004 mmol). Purity > 95% by HPLC M+hT 780.19.

Example 70
(Figure Removed)


propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (70)
The procedure described in example 65 was followed but with the use of 63 instead of 59, followed by hydrolysis of the ethyl ester as described in example 66 which gave the title compound (12.4 mg, 0.02 mmol). Purity > 95% by HPLC M+H* 700.16.
Example 71
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-(1,1-Dioxo-tetrahydro-1-A,6-thiophen-3-yl-carbamoyl)-
2,2-dimethyl-propylcarbamoyl]-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-
carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (71)
The procedure described In example 65 was followed but with the use of 64 instead
of 59, followed by hydrolysis of the ethyl ester as described in example 66 which gave
the title compound (13 mg, 0.014 mmol). Purity > 95% by HPLC M+hT 748.13.
Example 72

(Figure Removed)
(4R)-1-(tert-butoxycarbonyl)-4-[(7-methoxy-2-phenyIquinolin-4-yl)oxy]-L-prolyl-A/1-(phenylsulfonyl)-L-norvalinamide (72)
To a solution of 10 (60 mg, 0.13 mmol) in DMF, HATU (124 mg, 0.325 mmol), diisopropylethylamine (114 \iL, 0.65 mmol) was added and agitated for 30 min at RT. A solution of 75 (0.157 rnmol) in DMF was added. The slurry was agitated for 16 hrs at RT followed by concentration to dryness. The residue was taken up in DCM and washed with NaHCOs (sat), and water. The organic layer was dried, concentrated and subjected to silica column chromatography (gradient elution from 100% DCM to 2%MeOH/DCM) to give the title compound (61 mg, 0.087mmol). Purity > 90% by HPLC. M+H+ 703.23.
Example 73
(Figure Removed)
(4f?)-4-[(7-methoxy-2-phenylquinonn-4-yl)oxyj-L-prolyl-A/1-(phenylsuIfonyl)-L-norvalinamide (73)
Compound 72 was kept in DCM-TFA 2:1 (2 ml) for 2.5 hr at RT. The solution was co-evaporated with toluene to dryness. Yield 100%. M+H 603.12
Example 74
(Figure Removed)
Carbamicacid, [(1$)-1-[[(phenylsulfonyl)amino]carbonyl]butyl]~, phenylmethyl ester (74)
To a stirred solution of Z-Nva-OH (150 mg, 0.59 nrimol) in THF (6 ml), GDI (400 mg, 2.4 mmol) was added. The slurry was agitated for 30 min at RT followed by the addition of DBU ( 200 \iL, 1.3 mmol) and a solution of benzenesulfonamide (250 mg, 1.59 mmol) in THF(2 ml). The mixture was stirred at 60 °C for 48 hrs followed by concentration to dryness. The residue was dissolved in MeOH and subjected to HPLC purification to give the title compound (118.5 mg, 0.304 mmol). Purity > 95% by HPLC. M-H+389.0, +Na412.96.
Example 75
(Figure Removed)
(2S)-2-Amino-N-(phenylsulphonyl)pentanamide (75)
Compound 74 was dissolved in MeOH (5 ml) followed by the addition of Pd/C and subjected to hydrogenation for 2 hrs. The slurry was filtered through celite, washed with MeOH and concentrated to dryness to give the title compound. Yield 100%. M+H+257.3.
Example 76
(Figure Removed)

4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-1,2-dicarboxylic acid 1-({1-[(cyclohexyl-methylcarbarnoyl-methyl)-carbamoyl]-2,2-dlmethyl-propyl}-amide)-2-[(1-phenyImethanesulfonyfaminocarbonyl-2-vinyl-cyclopropyl)-amide] (76) To solution of 42 (8.7 mg, 0.011 mmol) in chloroform (1 ml) was added a-toluenesulfonamide (7 mg, 0.04 mmol) followed by diisopropylethylamine (21 pL, 0.12 mmol). The solution was stirred at RT for 10 min and then at -20 °C for 30 min. PyBOP (46.5 mg, 0.08 mmol) was then added as a solid. The solution was kept at -20 °C for 48 hours. The solution was then poured into aqueous NaHCO3 (sat.) and washed with water. The organic layer was dried, concentrated and subjected to purification by HPLC, affording the title compound as a white solid (2.8 mg, 0.0049 mmol), Purity by HPLC > 95%, M+H* 936.26.
Example 77
(Figure Removed)
N-(2-Hydroxy-indan-1-yl)-2-[4-(6-methoxy-3-phenyl-naphthalen-1-yloxy)-2-(1-methanesulfonylarninocarbonyl-2-vinyl-cyclopropyl)-pyrrolidin-1-yl]-3,3-dimethyI-butyramide (77)
The title compound was prepared as described in example 76, using 14 as carboxylic acid starting material and methanesulfonamide instead of a-toluenesulfonamide. Yield 13%, Purity by HPLC > 95%, M+H+839.16.
Example 78
(Figure Removed)
4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-1,2-dicarboxylic acid 1 -{[1-
(cyclohexylmethyI-carbamoyl)-2-methyl-propyl]-amide}2-[(1-
phenylmethanesulfonylaminocarbonyl-2-vinyl-cyclopropyl)-amide] (78)
The title compound was prepared as described in example 76,
using 23 as carboxylic acid starting material. Yield 2%. Purity > 95% by HPLC. M+H+
865.28.
Example 79
(Figure Removed)
4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrroIidine-1,2-dicarboxylic acid (cyclohexylmethyi-carbamoyl)-2-methyl-propyl]-amide}2-[(1-phenylmethanesulfonylaminocarbonyl-butyl)-amide] (79)
The title compound was prepared as described in example 76, using 52 as carboxylic acid starting material. Yield 8%. Purity > 95% by HPLC. M-f-H+855.28.
Example 80
(Figure Removed)
4-(7-Methoxy-2-phenyI-quinolin-4~yloxy)-pyrrolidine-1,2-dicarboxylic acid 2-[(1-benzenesulfonylaminocarbonyl-butyl)-amide] 1 -ff1 -(cyclohexylmethyl-carbamoyl)-2-methyl-propyl]-amide} (80)
The title compound was prepared as described in example 76, using 52 as carboxylic acid starting material and benzensulpnonamide instead of a-toluenesulfonamide. Yield 21.5%. Purity > 95% by HPLC. M+H*841.28.
Example 81
(Figure Removed)
4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-1,2-dicarboxylic acid 2-[(1-benzenesulfonylaminocarbonyl-2-vinyl-cyclopropyl)-amide] 1-({1~[(cyclohexyl-methylcarbamoyl-methyl)-carbamoyl]-2,2-dimethyl-propyl}-amide) (81)
The title compound was prepared as described in example 76, using benzenesulfonamide instead of a-toluenesulfonamide. Yield 26 %. Purity by HPLC > 95 %,M+H* 922.23.
Example 82
(Figure Removed)
4-(7-Methoxy-2-phenyl-quinolin-4-yIoxy)-pyrrolidine-1,2-dicarboxylic acid 2-[(1-benzenesulfonylaminocarbonyl-butyl)-amide] 1-{[1-(2-hydroxy-indan-1-ylcarbamoyl)-2-methyl-propyi]-amide} (82)
To a solution of 73 (24.1 mg, 0.04 mmol) in DCM (2 ml), was added a large excess of NaHCOs (s) and a solution of phosgene in toluene (50 jxL, 0.096 mmol). After 1O min of agitation the slurry was filtered and concentrated to dryness. The solid was redissolved in DCM and a large excess of NaHC03 (s) and 2-amino-A/-(2-hydroxy-indan-1-yl)-3-methyl-butyramide, described in example 35, (0.1 mmol) was added. The slurry was agitated for 40 hrs at RT. The slurry was filtered, concentrated and subjected to HPLC purification, to give the title compound (1.6 mg, 0.0018 mmol). Purity > 95% by HPLC. M+H+ 877.21.
Example 83
(Figure Removed)
4-(7~Methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-1,2-dicarboxylic acid 2-[(1-
benzenesulfonylaminocarbonyl-butyl)-amide] 1 -({1 -[(cyclohexyl-methylcarbamoyl-
methyl)-carbamoyl]-2,2-dimethyl-propyl}-amide) (83)
The title compound was prepared as described in example 82 but using 21 instead of
2-amino-/V-(2-hydroxy-indan-1-yl)-3-methyl-butyramide. Yield 2%. Purity > 95% by
HPLC.M+hT912.25.
Example 84
(Figure Removed)
(1 R,2S)-H[(4R,2S)1 -(1 -(1 S)-Hydroxymethyl-2,2-dimethyl-propylcarbamoyl)-4-(7-
methoxy-2-phenyl'quinolin-4-yloxy)-pyrro!idine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid ethyl ester (84)
Treatment of compound 12 as described for the preparation 13 but with the use of
(S)-tert-leucinol instead of 2-amino-N-(2~hydoxy-indan-1~yl) 3,3-dimethyl-butyramide
provided the title product. M+H+ 645.2.
(Figure Removed)
phenyl-qulnolin-4-yloxy)-pyrrolldlne-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyllc acid ethyl ester (85)
To a stirred solution of compound 84 (64 mg) in dichloromethane Dess-Martin periodinane (80 mg) was added at ambient temperature. After 4 hrs the slurry was filtered through basic alumina and concentrated to dryness, M+H+ 643.2.
Example 86
(Figure Removed)
(1R,2S)-H[(4R,2S)1-{1-[((1S,2R)-2-Hydroxy-indan-1-ylamino)-methyl]-2,2-dimethyl-
propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-
amino}-2-vinyI-cyclopropanecarboxylic acid ethyl ester (86)
To a solution of compound 85 in THF (2 ml) and HOAc (0.5 mL) polystyrene bound
cyanoborohydride (2.36 mmol/g, 100 mg) and (1S,2R)-1-aminoindan-2-ol (18 mg)
was added and agitated for 4 hrs. The mixture was filtered, concentrated and purified on a prep. HPLC. Purity by HPLC >90%. M+H+ 776.5
Example 87
(Figure Removed)

(1R,2S)-H[(4R,2S)H1-[((1S,2R)-2-Hydroxy-indan-1-ylamino)-methyl]-2,2-dimethyl-
propyIcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-y!o3(y)-pyrrolidine-2-carbonyrj-
am!no}-2-vinyl-cyclopropanecarboxyl!c acid (87)
To a solution of compound 86 in THF (2 ml) and MeOH (1 ml) 1N LiOH (0.2 mL)
was added and the solution was kept at 60 °C for 1.5 hrs. The slurry was neutralized
with 1N HCI to pH 7, concentrated and purified on a prep. HPLC giving pure product
by HPLC >95%. M+H* 748.4.
Example 88
(Figure Removed)

(1R,2S)-1-{[(4R,2S)1-(1-{[(1S)-(Cyclohexyl-methylcarbamoyl-methy!)-amino]-methyl}-2,2-dimethyl-propylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino)-2-vinyl-cyclopropanecarboxyIic acid (B8)
Treatment of compound 85 as described for the preparation of 86 but with the use of 2-amino-2-cyclohexyl-N-metyl-acetamide (17 mg) instead of (1S,2R)-1-aminoindan-2-ol followed by hydrolysis of the ethyl ester as described in example 87 provided the title product. Purity by HPLC >95%. M+H* 769.5
Example 89
(Figure Removed)
Acetic acid (1S,2R)-1-((2S)-2-amino-3,3-dimethyl-butyrylamino)-indan-2-yl ester (89) A solution of compound 17 (4g) was kept in pyridine-acetic anhydride 2:1 for 30 min. DCM was added and the solution was washed with citric acid (aq) and NaHCO3 (aq). The organic layer was concentrated to dryness which gave the acetylated product >90% pure by HPLC. The afforded compound was then kept in a solution of 30% TFA in DCM for 1.5 hrs and then concentrated to dryness. Co-evaporation twice from toluene gave the title product >90% pure by HPLC.
Example 90
(Figure Removed)
(2S,4R)-2-((1S,2R)1-Ethoxycarbonyl-2-vinyl-cyclopropylcarbamoyl)-4-hydroxy-pyrrolidine-1-carboxylic acid tert.butyl ester (90)
A solution of HATU (6 g), diisopropylethylamine (6.8 mL), (1R,2S)-1-amino-2-vinyl-cyclopropanecarboKylic acid ethyl ester (1.5 g) and BOC-L-hydroxyproline (1.6 g) in
dichloromethane was stirred for 1 hrs. The mixture was extracted with DCM-NaHCO3 (aq) dried and concentrated. HPLC purity ca 90% M+H+ 369.1.
Example 91
(Figure Removed)
(1S,2R)-1-[(2S,4RM4-Hydroxy-pyrrolidine-2-carbonyl)-amino]-2-vinyl-cyclopropanecarboxyilc acid ethyl ester (91).
Compound 90 was kept in 30% trifluoroacetic acid in dichloromethane and 1% MeOH for 2 hrs before it was concentrated to dryness. The residue was re-dissolved in dichloromethane and during stirring 1N NaOH was added to pH 10-11. The organic layer was separated and concentrated which gave 1.6 g of the title product. HPLC purity ca. 90% M+H+ 269.1.
Example 92
(Figure Removed)
(1 R,2S)-1-({(2S,4R)-1 -[(1 S)-1 -((1 S,2R)-2-Acetoxy-indan~1 -ylcarbamoyl)-2,2-dimethyl-propylcarbamoyl]-4-hydroxy-pyrrolidine-2-carbonyl}-amino)-2-vinyl-cyclopropanecarboxylic acid ethyl ester (92).
To a stirred solution of compound 89 (1.81 g) in acetonitrile at 0 °C solid NaHCOs (800 mg) and p-nftrophenychlorocarbonate (1.2 g) was added. The slurry was taken up to ambient temperature and stirred for another 30 min. To this slurrry a slution of compound 91 (1.6 g) in acetonitrile (5 mL) diisopropylethylamine (1mL) was added. After 10 min the resulting mixture was concentrated, re-dissolved in ethyl acetate and
washed with K2CO3 (aq) and then with 0.5 N HCI. Dried and concentrated which gave a >80% pure product by HPLC M+H* 599.6
Example 93
(Figure Removed)

(1R,2S)-1-({(2S,4R)-1-[(1)-1-((1S,2R)-2-Acetoxy-indan-1-ylcarbamoyl)-2,2-dimethyl-propyicarbamoyl]-4-phenylcarbamoyloxy-pyrrolidlne-2-carbonyl}-amino)-2-vinyl-cyclopropanecarboxylic acid ethyl ester (93)
To a stirred solution of compound 92 (20mg) in DCM and solid KzCOa (200 mg) 20% phosgene in toluene (1 mL) was added. After 6 hrs the slurry was filtered and concentrated to dryness. To this residue a mixture of aniline (30 mg) DCM (3 mL) and solid NaHCOa (50 mg) was added and agitated for 10 hrs. The mixture was filtered, concentrated and purified on a prep. HPLC which gave the title product, >95% pure M+H+718.6.
Example 94
(Figure Removed)
(1R,2S)-1-({(2S,4R)-1-[1-((1S,2R)-2-Hydroxy-indan-1-yicarbamoyl)-2l2-dimethyl-propylcarbamoyl]-4-phenylcarbamoyloxy-pyrroIidine-2-carbonyl}-amino)-2-vinyl-cyclopropanecarboxylic acid (94)
To a solution of compound 93 in THF-MeOH 2:1 (3 ml) was added 1N LiOH (0.2 ml). The solution was heated to 60 °C for 2 hrs. After cooling to ambient temperature acetic acid (0.5 ml) was added and the solution was concentrated to dryness. The remaining residue was purified on a prep. HPLC which gave the title product >95% pure M+H+ 648.5.
Example 95
(Figure Removed)
(5S,3R)-3,4-Dihydro-1 H-isoquinoline-2-carboxylic acid 5-((1 R,2S)-1-carboxy-2-vinyl-
cyclopropylcarbarnoyl)-1-[1-((1SI2R)-2-hydroxy-indan-1-ylcarbamoyl)-2,2"dimethyl-
propylcarbamoyl]-pyrrolidin-3-yl ester (95)
Treatment of compound 92 as described for the preparation of 93 but with the use of
1,2,3,4-tetrahydroHsoquinoline instead of aniline followed by hydrolysis of the ethyl
ester as described in example 94 gave the title compound. Purity >90%. M+H+ 688.6.
Example 96
(Figure Removed)

(5S,3R)-3,4-Dihydro-2H-quinoline-1-carboxylic acid 5-((1 R,2S)-1-carboxy-2-vinyl-
cyclopropylcarbamoyl)-1-[1-((1S,2R)-2-hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyl-
propylcarbamoyl]-pyrrolidin-3-yl ester (96)
Treatment of compound 92 as described for the preparation of 93 but with the use of
1,2,3,4-tetrahydro-quinoline instead of aniline followed by hydrolysis of the ethyl ester
as described in example 94 gave the title compound. Purity >90%. M+H+ 688.6.
Example 97
(Figure Removed)
(1R,2S)-1-{[(2S,4R)-1-[(1S)-1-((1S,2R)-2-Hydroxy-indan-1-ylcart>arnoyl)-2I2-dirnethyl-
propylcarbamoyl]-4-(pyridin-3-yImethylcarbamoyloxy)-pyrroIidine-2-carbonyl]-amino}-
2-vinyl-cyclopropanecarboxylic acid (97)
Treatment of compound 92 as described for the preparation of 93 but with the use of
2-pyridine-3-yl-ethy!amine instead of aniline followed by hydrolysis of the ethyl ester
as described in example 94 gave the title compound. Purity >90%. M+H+ 663.5.
Example 98
(Figure Removed)

(1R,2S)-H[(2Sl4R)-1-[(1S)-1-((1S)2R)-2-Hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyl-
propylcarbamoyl]-4-(methyl-phenethyl-carbamoy!oxy)-pyrrolidine-2-carbonyl]-amino}-
2-vinyl-cyclopropanecarboxylic acid (98)
Treatment of compound 92 as described for the preparation of 93 but with the use of
N-methylphenethylamlne instead of aniline followed by hydrolysis of the ethyl ester as
described in example 94 gave the title compound. Purity >90%. M-t-H* 690.6.
Example 99
(Figure Removed)
(1R,2S)-1-({(2S,4R)-4-Benzylcarbamoyloxy-1-[(1S)-1-((1S,2R)-2-hydroxy-indan-1-
ylcarbamoyl)-2,2-dimethyl-propylcarbamoyl]-pyrrolidine-2-carbonyl}-amino)-2-vinyl-
cyclopropanecarboxylic acid (99)
Treatment of compound 92 as described for the preparation of 93 but with the use of
benzylamine instead of aniline followed by hydrolysis of the ethyl aster as described
in example 94 gave the title compound. Purity >90%. M+H+ 662.4.
Example 100
(Figure Removed)
(1 R,2S)-1 -({(2S,4R)-1 -[(1 S)-1 -((1 S,2R)-2-Hydroxy-indan-1 -ylcarbamoyl)-2,2-dimethyl-
propylcarbamoyl]-4-phenethylcarbamoyloxy-pyrrolidine-2-carbonyl}-amino)-2-vinyl-
cyclopropanecarboxylic acid (100)
Treatment of compound 92 as described for the preparation of 93 but with the use of
phenthylamine instead of aniline followed by hydrolysis of the ethyl aster as described
in example 94 gave the title compound.Purity >90%. M+H* 676.5.
Example 101
(Figure Removed)
(1 R,2S)-1 -({(4/?)-1 -{[2-(terf-butoxycarbonyl)hydrazino]carbonyl}-4-[(7-methoxy-2-phenylquinolln-4-yl)oxy]-L-prolyI}amino)-2-vinylcyclopropanecarboxylic acid ethyl ester (101)
To a solution of ferf-butyl carbazate (0.3 mmol) and p-nitro phenyl chloroformate (0.3 mmol) in acetonitrile (6 ml) was added sodium hydrogen carbonate (0.48 mmol) as solid. The solution was stirred at RT for 5 hrs and then cooled down to 0 °C. Compound 62 (0.3 mmol) dissolved in acetonitrile (10 ml_) was mixed together with diisopropylethylamine (0.75 mmol) at 0 °C, and then added to the previous solution. The mixture was stirred at RT overnight and then concentrated to dryness. The residue was dissolved in DCM and then washed with citric acid pH 4, followed by

NaHCOa (aq) and water, dried over anhydrous sodium sulphate, filtrated and concentrated to dryness, The crude was dissolved in DCM and purified by column chromatography eluted with 0.1 to 0.2% MeOH/DCM to yield the title compound (101 mg). Purity >95% by HPLC, M+H+ 660.1.
Example 102
(Figure Removed)
(1 R,2S)-1 -({(4R)-1 *{[2-(terf-butoxycarbonyl)hydrazino]carbonylMHF-rnethoxy-2-phenylquinolin-4-yl)oxy]-L-prolyl}amino)-2-vinylcycIopropanecarboxylic acid (159) Method A: To a solution of compound 101 (0.0115 mmol) in THF-MeOH 2:3 (2 ml) was added 1M LiOH (10 equiv) The solution was kept at 50 °C for 60 min. After cooling to RT, HOAc (20 equiv) was added followed by toluene (2 ml) and then concentrated to dryness. The residue was taken up in MeOH and then purified by Prep LCMS which gave the title compound (0.7 mg). Purity >95% by HPLC M+H+ 732.2.
Method B: To a solution of ferf-butyl carbazate (0.07 mmol) and p-nitrophenyl chloroformate (0.07 mmol) in acetonitrile (3 ml) was added sodium hydrogen carbonate (0.112 mmol) as a solid. The solution was stirred at RT for 2.5 hrs and then cooled to 0 °C. Compound 103 (described below) (0.07 mmol) dissolved in acetonitrile (10 ml) was mixed together with diisopropylethylamine (0.175 mmol) at 0 °C, and then added to the previous solution. The mixture was stirred at RT overnight and then concentrated to dryness. The crude material was dissolved in MeOH and purified by Prep LCMS which gave the title compound (4.8 mg). Purity >95% by HPLC M+H* 632.2
Example 103



(Figure Removed)

(1R,2S)-1-{[(2S,4R)-4-(7-Methoxy-2-phenyl-quinoIin-4-yloxy)-pyrroridine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (103)
To a solution of compound 12 (0.067mmol) in THF-MeOH 2:3 (2 ml) was added 1M LiOH 10 equiv. The solution was kept at 50 °C for 2.5 hrs. After cooling to RT, HOAc 20 equiv. was added followed by toluene (2 ml) and then concentrated to dryness. The residue was taken up in DCM and filtered form the salts which gave the title compound (0.07 mmol). Purity >95% by HPLC M+I-T 474.
Example 104
(Figure Removed)

-(hydrazinocarbonyl)-4-[(7-methoxy-2-phenylquinolin-4-yl)oxy]-L-prolyl}amino)-2-vinyIcyclopropanecarboxyllc acid (104)
Compound (102) was kept in TFA-DCM 1 :2 (3 ml) at RT for 60 min. Toluene (1 ml) was added. The sample was co-evaporated to dryness which gave the title compound (10.5 mg) as the trifluoracetic acid salt. Purity by HPLC >95%. M+l-f532.
Example 105
(Figure Removed)

(1 R,2S)-1 -({(4R)-1 -(hydrazinocarbonyl)-4-[(7-methoxy-2-phenylquinolin-4-yl)oxy]-L-prolyl}amino)-2-vinylcyclopropanecarboxylic acid ethyl ester (105) Compound 101 (50 mg) was kept in TFA-DCM 1:2 (3 ml) at RT for 60 min. Toluene (1 ml) was added. The sample was co-evaporated to dryness and then taken up in DCM and washed with KzCOa, dried over anhydrous sodium sulphate and concentrated to dryness which gave the title compound (41.8 mg). Purity by HPLC >95%. M+H+560.
Example 106
(Figure Removed)
)-1 -({(4/RJ-1 -[(2-Benzylhydrazino)carbonyl]-4-[(7-methoxy-2-phenylquinolin-4-yl)oxy]-L-prolyl}amino)-2-vlnylcyclopropanecarboxylic acid etyl ester (106) To a solution of compound 105 (0.037mmol) in MeOH:THF (4:1) was added benzaldehyde (0.0448 mmol). The solution was stirred at RT for 18 hrs. Borane-pyridine complex (0.37 mmol) was added followed by HCI (37%, 400 ul). The solution was stirred for 1.5 hrs and then filtrated and concentrated to dryness. The crude material was dissolved in MeOH and purified by Prep LCMS which gave the title compound (0.01 mmol). Purity by HPLC >95%. M+H+650.
Example 107
(Figure Removed)
(1R,2S)-1-({(4f?)-1-[(2-benzylhydrazino)carbonyl]-4-[(7-methoxy-2-phenylquinolin-4-yl)oxy]-L-prolyl}amino)-2-vinylcyclopropanecarboxylic acid (164107 To a solution compound 106 (0.0101 mmol) in THF-MeOH 2:3 (3 ml) was added 1M LiOH 10 equiv. The solution was kept at 50 °C for 18 hrs. After cooling to RT the sample was neutralized with HCI and concentrated to dryness. The crude material was dissolved in DCM (2 rn!) and a solution of TFA: TES 1:1 (1 ml) was added. The mixture was stirred for 3 hrs at RT and then concentrated to dryness. The crude material was dissolved in MeOH and purified by Prep LCMS which gave the title compound (0.6 mg). Purity by HPLC >95%. M+H+622.
Example 108
(Figure Removed)
(1R,2S)-H[(2S, 4R)-1-((1S)-1-Azidomethyl-3-methyi-butylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (108)
(Figure Removed)
i) (2S)-Methanesuiphonic acid 2-tert.butoxycarbonylamino-4-methyl-pentyl ester To a solution of ((1S)-1-hydroxymethyl-3-methyl-butyl)-carbamicacid tert-butyl ester (25 g, 115 mmoi) in dichloromethane (500 ml) cooled by an ice-water bath was successively added diisopropylethylamine (35.7 g, 276 mmol) and methanesulphonyl chloride (15.81 g, 138 mmol). The resulting solution was stirred over night during which time the mixture was allowed to gradually warm up to ambient temperature. The mixture was washed successively with water, 10 % citric acid (aq), water and saturated NaHCOs (aq), then dried with Na2S04 and concentrated to a brown solid (32.6 g, 96 %) which was used in the next reaction without further purification.
(Figure Removed)
ii) ((1S)-1~Azidomethyl-3-methyl-butyl)-carbamic acid tert.butyl ester The mesylate from step i (32.6 g, 1 10 mmol) was treated with sodium azide (21 .45 g, 330 mmol) in DMF at 80 °C for 24 hrs. The solvent was evaporated, the residue was taken up in DCM, filtered and washed with saturated NaHCO3 (aq). The solution was dried with Na2SC>4 and concentrated to a brown oil which was purified by flash chromatography using a gradient of ethyl acetate and hexane to afford the title compound as a white solid (19.55 g, 73 %).
(Figure Removed)
iii) (1 S)-1-AzidomethyI-3-rnethyl-butylamine
((1S)-1-Azidomethyl-3-methyl-butyl)-carbamic acid tert-butyl ester (9.64 g, 39.78 mmol) was treated with TFA (30 ml) in DCM (150 ml) for 3 hrs, the mixture was evaporated under reduced pressure and the residue was dissolved in ethyl acetate and washed with aqueous 1 M K2C03, dried with Na2S04 and concentrated to a yellow liquid (4.55 g, 80 %).
Compound 12 was treated with phosgene as described in example 13 which gave the corresponding chlorocarbamate compound. The afforded chlorocarbamate (568 mg, 1.13 mmol) was dissolved in a solution of DCM-THF (1:1, 10 ml) and (1S)-1-

azidomethyl-3~methyl-butylamine (401 mg, 2.82 mmol) and a large excess of NaHCOa (s) was added. The resulting mixture was stirred for 18 hrs, filtered and washed with dilute citric acid (aq, pH 5). The organic layer was dried with Na2SO4and evaporated to afford the desired product as a light yellow oil (837 mg, 99 %) sufficiently pure to be used in the next step. M+H+670.1.
Example 109
(Figure Removed)
(1R, 2S)-1-{[(2S, 4R)-1-((1S)-1-Aminomethyl-3-methyl-butylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (109)
A solution of 108 (717 mg, 1.07 mmol) in THF (25 ml) was shaken together with PS-triphenylphosphine resin (diphenylphosphino polystyrene) (3.24 g, 1.65 mmol PPha/g) and methanol (2.5 ml) for 78 hrs. The mixture was filtered and the polymer was washed repeatedly with DCM and methanol. The combined filtrates were evaporated to yield the title compound as a light beige solid foam (685 mg, 99 %) with more than 95 % purity as determined by reversed phase HPLC. M+H* 644.1.
General procedure 1A for the preparation of compounds 110-116 To a solution of the acyl chloride (0.075 mmol) in DCM (0.5 ml) was added NaHCO3 (s) (60 mg, 07 mmol) and a solution of the amine 109 (25 mg, 0.037 mmol) in THF (1 ml). The resulting mixture was stirred at room temperature over night, filtered and then shaken in the presence of PS-trisamine resin (tris-(2-aminoethyI) aminomethyl polystyrene) (3.91 mmol/ g, 50 mg, 0.2 mmol) for 5 hrs. The mixture was filtered and
evaporated. The resulting solid residue was dissolved in MeOH-THF (2:1, 1.5 ml) and treated with 1 M LiOH (aq) (170 pi) at 50 °C between 2 and 16 hrs. The reaction was monitored by HPLC-MS. The mixture was acidified with acetic acid and evaporated to dryness. The residue was dissolved in methanol and purified by reversed phase HPLC.
General procedure i B for the preparation of compounds 11.P-116 To the acid (0.039 mmol) was successively added a solution of HATU (14.7 mg, 0.039 mmol) in DMF (0.5 ml), a solution of the amine 109 (20 mg, 0.031 mmol) in DMF (0.5 ml) and diisopropylethylamine (30 ul, 0.155 mmol). The resulting mixture was stirred for 16 hrs then the solvent was evaporated and the residue was dissolved in DCM and washed with water and aqueous saturated NaHCO3. The solvent was evaporated and the residue was dissolved in methanol-THF (2:1,1.5 ml). To this was added 1 M LiOH (aq) (155 ul) and the mixture was stirred at 60 °C for 3-5 hrs. Glacial acetic acid (50 ul) was added and the mixture was concentrated, dissolved in methanol and purified by reversed phase HPLC.
Example 110
(Figure Removed)
(1R, 2S)-1-{[(2S, 4R)-H(1S)-1-[(3-Fluoro-benzoylamino)-methyl]-3-
methyIbutylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-
carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (110)
General procedure 1A was followed using 3-fluorobenzoyl chloride (12 mg) as acyl
chloride which gave the title compound as a solid (13.6 mg, 50 %). M+HT738.1.
Example 111
(Figure Removed)
(1R, 2S)-H[(2S, 4R)-4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1-((1 S)-3-methyl-1-{[(pyridine-3-carbonyl)-amino]-methyl}-butylcarbamoyl)-pyrrolldine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (111)
General procedure 1A was followed using nicotinoyl chloride (10.5 mg) as acyl chloride which gave the title compound as a solid (10 mg, 37 %). M+H*721.1.
Example 112
(Figure Removed)
(1 R, 2S)-H[(2S, 4RH-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1 -((1 S)-3-methyl-1-{[(pyrazine-2-carbonyl)-amino]-methyl}-butylcarbamoyl)-pyrrolidine-2-carbonyl]-arnino}-2-vinyl-cyclopropanecarboxylic acid (112)
General procedure 1B was followed using pyrazine-2-carboxylic acid (5 mg) as acid which gave the title compound as a solid (5.7 mg, 25 %). M+H'I722.1.
Example 113
(Figure Removed)

(1R, 2S)-H[(2S, 4R)-4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1-((1 S)-3-methyI-1-{[(thiophene-3-carbonyl)-amino]-methyl}-butylcarbamoyl)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyolopropanecarboxylic acid (113)
General procedure 1A was followed using thiophene-3-carbonyl chloride (11 mg) which gave the title compound as a solid (4.3 mg, 16 %). M+H+726.1.
Example 114
(Figure Removed)
, 2S)-H[(2S, 4R)-4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1-((1 S)-3-methyl-1-{[(tetrahydro-furan-2-carbonyl)-amino]-methyl)-butylcarbamoyl)-pyrrolidine-2-carbonyl]-amino}*2-vinyl-cyclopropanecarboxylic acid (114)
General procedure 1B was followed using tetrahydrofurane-2-carboxylic acid (4.5 mg) as acid which gave the title compound as a solid (7.9 mg, 36 %). M+H+714.1.
Example 115
(Figure Removed)
(1 R, 2S)-1 -fl(2S, 4RH-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1 -((1 S)-3-methyI-1 -{[(5-
phenyl-oxazole-4-carbonyl)-amino]-methyl}-butylcarbamoyl)-pyrrolidine-2-carbonyl]-
amino}-2-vinyl-cyclopropanecarboxylic acid (115)
General procedure 1B was followed using 5-phenyl-oxazole-4-carboxylic acid (7.5
mg) as acid which gave the title compound as a solid (7.5 mg, 31 %). M+H+787.1.
Example 116
(Figure Removed)
(1 R, 2S)-1-{[(2S, 4R)-1-((1 S)-1-{[(Benzofuran-2-carbonyl)-amino]-methyl}-3-methyl-butylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyiic acid (116)
General procedure 18 was followed using benzofuran-2-carboxylic acid (6.5 mg) as acid which gave the title compound as a solid (5.4 mg, 23 %). M+H*760.1.
General procedure 2 for the preparation of compounds 117-119 To a solution of the suiphonyl chloride (0.075 mmol) in DCM (0.5 ml) was added NaHCO3 (s) (60 mg) and a solution of the amine 109 (25 mg, 0.037 mmol) in THF (1 ml). The resulting mixture was stirred at room temperature for 18 hrs, filtered and then
shaken with PS-trisamine (tris-(2-aminoethyl)aminomethyl polystyrene, 3.91 mmol/ g, -50 mg) for 5 hrs.
The mixture was filtered and the polymer was washed successively with DCM, THF and methanol. The solid residue resulting from evaporation of the combined filtrates was dissolved in MeOH-THF (2:1,1.5 ml) and treated with 1 M LiOH (aq) (170 ul) at 50 °C for reaction times varying from 18 hrs to one week depending on the actual structure. The reaction was monitored by HPLC-MS. The mixture was acidified with acetic acid and evaporated to dryness. The residue was dissolved in methanol and purified by reversed phase HPLC.
Example 117
(Figure Removed)
(1R, 2S)-1 -({(2S, 4R)-4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1 -[(1 S)-3-methyl-1 -
(phenylmethanesulphonylamino-methyl)-butylcarbamoyl]-pyrrolidine-2-carbonyl}-
amino)-2-vinyl-cyclopropanecarboxylic acid (117)
General procedure 2 was followed using a-toluenesulphonyl chloride (14 mg) as
sulphonyl chloride which gave the title compound as a white solid (4.9 mg, 17 %).
M+H* 770.1.
> Example 118
(Figure Removed)
(1 R, 2S)-1 -[((2S, 4R)-4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-1 -{(1 S)-3-methyl-1 -[(5-
methyl-isoxazole-4-sulphonyIamino)-methyl]-butylcarbamoyl}-pyrrolidine-2-carbonyl)-
amino]-2-vinyl-cyclopropanecarboxylic acid (118)
General procedure 2 was followed using 5-methyl-isoxazole-4-sulphony! chloride (14
mg) as sulphonyl chloride which gave the title compound as a white solid (1 .6 mg, 6
%). M+H+761.0.
Example 1 1 9
(Figure Removed)
S)-1-[(5-lsoxazol-3-yl-thiophene-2-sulphonylamino)-methyl]-3-methyl-butylcarbamoylH~(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino)-2-vinyl-cyclopropanecarboxylic acid (119) General procedure 2 was followed using 5-isoxazo!-3-yl-thiophene-2-sulphonyl chloride (19 mg) as sulphonyl chloride which gave the title compound as a white solid (3.0 mg, 10 %). M+H+828.98.
Example 120

(Figure Removed)
H[1-(N'-tert.Butoxycarbonyl-N-hept-6-enyl-hydrazlnocarbonyl)-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (120)
Compound 12 (200 rng, 0.4 mmol) was dissolved in tetrahydrofuran (10 ml). A tea­spoon of sodium hydrogencarbonate was added, followed by phosgene (1.8 ul, 1.9 M in toluene). The reaction mixture was stirred for 30 min and filtrated. The solvent was evaporated and the crude chloride was re-dissolved in dichloromethane (10 ml). Sodium hydrogencarbonate (1 tea-spoon) and A/'-hept~6-enyl-hydrazinecarboxylic acid ferf.butyl ester (182 mg, 0.8 mmol). The reaction mixture was stirred at room temp, for 40 h. and then filtrated and purified by silica chromatography (1 % methanol in ether ->• 2 % methanol in ether) to give pure title product (240 mg, 79 %).
Example 121
(Figure Removed)
14-tert.Butoxycarbonylamino-18-(7-methoxy-2-phenyI-quinolin-4-yloxy)-2,15-dioxo-3,14,16-triaza-tricyclo[14.3.0.0*4,6*]nonadec-7-ene-4-carboxylic acid ethyl ester (121)
Compound 120 (20Qmg, 0.26 mmol) was dissolved in degassed dichloromethane (30 ml), Hoveyda - Grubbs catalyst II generation (16 mg, 0.026 mmol) was then added and the mixture was refluxed under argon atmosphere overnight. The solvent was then evaporated and the crude product was purified by silica chromatography (1 % methanol In ether) which gave 39 mg (20 %) of the title product. MS (M+H*) 728.2
Example 122
(Figure Removed)
14-tert.Butoxycarbonylamino-18-(7-methoxy-2"-phenyl-quinolin-4-yloxy)-2,15-dioxo-3,14,16-triaza-tricyclo[14.3.0.0*4,6*]nonadec-7-ene-4-carboxylicacid (122) Compound 121 (39 mg, 0.054 mmol) was dissolved in tetrahydrofuran (3.5 rnl), water (1.75 ml) and methanol (1.75 ml). Lithium hydroxide (430 ul, 1 M in water) was then added and the reaction was stirred at room temperature for 24 h. The volume was reduced to half and water (10 ml) was added. Acidification (pH=5) followed by extraction with chloroform gave 34 mg (90 %) of the pure acid 179. MS (M+H*) 700.2
Example 123
(Figure Removed)
H[1-(N'-tert.Butoxycarbonyl-N-hex-5-enyl-hydra2inocarbonyl)-4-(7-methoxy-2-
phenyI-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cycloprpanecarboxylic
acid ethyl ester (123)
The title compound was prepared from compound 12 (800 mg, 1.6 mmol) and
A/'-hex-5-enyl-hydrazinecarboxylic acid tert.butyl ester (620 mg, 2.9 mmol) according
to the procedure described In Example 120 which gave 1 g (85 %). MS (M+H+)
742.37
Example 124
(Figure Removed)
13-tert.Butoxycarbonylamino-17-(7-methoxy-2-phenyl-quinolin-4-yloxy)-2,14-dioxo-3,13,15-triaza-tricyclo[13.3.0.0*4,6*]octadec-7-ene-4-carboxylic acid ethyl ester (124) Treatment of compound 123 (400 mg, 0.54 mmol) according to the procedure described in example 121 gave a crude product. Purification by silica gel chromatography (1 % methanol in ether) gave the title product (67 mg, 17 %). MS (M+H*) 714.29
Example 125
(Figure Removed)

13-tert.Butoxycarbonylamino-17-(7-methoxy-2-phenyl-quinolin-4-yloxy)-2,14-dioxo-3,13,15-triaza-tricyclo[13.3.0.0*4,6*]octadec-7-ene-4-carboxylic acid (125) The title compound was prepared from compound 124 (67 mg, 0.09 mmol) by the same procedure as described for 122 which gave 46 mg (71 %) of the pure acid. Chloroform was replaced by 1, 2-dichloroethane in the extraction step for the preparation of this compound. MS (M+H4) 686.33
Example 126
(Figure Removed)
13-tert.Amino-17-(7-methoxy-2-phenyl-quinolin-4-yloxy)-2,14-dioxo-3,13,15-triaza-tricyclo[13.3.0.0*4j6*]octadec-7-ene-4-carboxylic acid (126) Compound 125 (10 mg) was dissolved in dichloromethane (4 ml). Trifluoromethanesulphonic acid (4 ml) was added and the mixture was left at 50° C for 6 hours. The solvent was removed and the residue was washed with acetonitrile which gave 3 mg of the pure title product (35 %). MS (M+H"1") 586.25
Example 127

(Figure Removed)
1 -{[1 -(1 -Methoxycarbonyl-oct-7-enylcarbamoyl)-4-(7-methoxy-2-phenyl-quinolin-4-
y!oxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester
(127)
The title compound was prepared from compound 12 (380 mg, 0.758 mmol) and 2-
aminononan-8-enyI*-carboxylic acid methyl ester (250 mg, 1.89 mmol) using the
conditions described in Example 120 which gave the pure product (405 mg, 75 %).
Example 128
(Figure Removed)
19-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-2,16-dioxo-3,15,17-triaza-
tricyclo[15.3.0.0*4,6*Jicos-7-ene-4,14-dicarboxylic acid 4-ethyl ester 14 methyl ester
(128)
Compound 127 (170mg, 0.2385 mmol) was dissolved in dichloromethane (40 ml) and
degassed by bubbling nitrogen for 20 min. Hoveyda - Grubbs catalyst II generation
(10 mg, 0.016 mmol, 6.7 mol %) was then added and the mixture was refluxed under
nitrogen atmosphere overnight. The solvent was then evaporated, catalyst and salts

were removed by flash chromatography (5% methanol in chloroform) and the crude product (120 mg, 73% yield, 85-90% purity) was used in next step MS (M+H+) 685
Example 129
(Figure Removed)
19-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-2,16-dioxo-3,15,17-triaza-tricyclo[15.3.0.0*4,6*]icos-7-ene-3,14-dicarboxylic acid 3-ethyl ester(129) Compound 128 (120 mg, 0.175 mmol) was dissolved in dioxane (9 ml) and water (6 ml). Lithium hydroxide (12 mg, 0.526 mmol) was added and the reaction was stirred at room temperature for 3.5 h. The mixture was acidified with acetic acid to pH=5, and co-evaporated with toluene. The crude product was used in the next step. MS (M+H+) 671
Example 130
(Figure Removed)
14-[(Cyclohexyl-methylcarbamoy!-methyl)-19-(7-methoxy-2-phenyl-quinolin-4-yloxy)-2,16-dioxo-3,15,17-triaza-tricyclo[15.3.0.0*4,6*]icos-7-ene-4-carboxy!ic acid 3-ethyl ester (130)
Compound 129 (crude, 100 mg), indanolamine (33 mg, 0.209 mmol) and Hunig's base (DIEA) (0.2 m!) were dissolved in DMF (14 ml). After stirring at 0 °C for 10 min
HATU was added. The reaction was monitored by LC-MS. After 5h conversion was 100%. DMF and DIEA were removed in vacuo. The residue was partitioned between ethyl acetate and water. The organic layer was washed with brine, dried and concentrated in vacuo. The crude yield was 120 mg, the purification by prep. HPLC gave 21 mg (25%) of title product. MS (M+H*) 802
Example 131
(Figure Removed)
14-[(Cyclohexyl-methylcarbamoyl-methyl)-19-(7-methoxy-2-phenyl-quinoIin-4-yloxy)-2,16-dioxo-3,15,17-triaza-tricyclo[15.3.0.0*4,6*]icos-7-ene-4-carboxylicacid (131) To a solution of the ester 130 (19 mg, 0.024 mmol) in the mixture of THF (0.2 ml) and methanol (0.3 ml) was added solution of LiOH (6 mg, 0.237 mmol) in 0.15 ml of water. The resulting mixture was stirred at 60 °C for 3.5h. After cooling to room temperature, acetic acid was added (30 eq). The mixture was co-evaporated with toluene. The residue was distributed between chloroform and water phases, the water one was extracted with chloroform and ethyl acetate, organic phases were combined, dried over sodium sulphate, evaporated to give 15 mg of pure product. MS (M+H*) 774
Example 132


(Figure Removed)

[14- Cyclopropanesulfonylaminocarbonyl~17-(7-methoxy-2-phenyl-quinolin-4-yloxy)-2,14-dioxo-3,13,15-triaza-trlcyclo[13.3.0.0*4,6*]octadec-7-en-13-yl]-carbamicacid ter.butyl ester (132)
To the acid 125 (19 mg, 0.028 mmol) in 0.5 ml of DMF was added 5.5 mg (0.044 mmol) of DMAP and 10.7 mg (0.056 mmol) of EDC. After 6.5h of stirring 20 mg of cyclopropylsulphone amide and 0.04 ml of DBU were added. The mixture was stirred overnight, acidified with 5% citric acid (in water) and extracted with ethyl acetate. Dried, evaporated, purified by 5% to 10% methanol in chloroform (or prep LC-MS) which gave 8 mg of the title compound (37%) MS (M+hT) 783
Example 133
(Figure Removed)
4-[2-(2-isopropylamino-thiazol-4-yl)-7-methoxy-quinolin-4-yloxy]-pyrrolidine-1,2-dicarboxylic acid 1-ttert.butyl ester (133)
To a stirred solution of N-Boc-trans-4-hydroxy-L-proline (221 mg, 0.96 mmol) in DMSO was added potassium tert.butoxide (320 mg, 2,9 mmol). After 1h 2-[2-isoprpylamino)-1,3-thiazol-4-yl]-7-methoxyquinolin-4-ol (319 mg, 0,96 mmol) was added and the mixture was stirred at 70 °C for 72 hours. The mixture was diluted with
water and extracted with ethyl acetate. The product was used without further purification. Yield 429 mg, 85%.
Example 134
(Figure Removed)
2-(1-Ethoxycarbonyl-2-vinyl-cyclopropylcarbamoyl)-4-[2-(2-isopropylamino-thiazoi-4-yl)-7-methoxy-quinolin-4-yloxy]-pyrrolidine-1-carboxylic acid tert.butyl ester (13-4) Compound 133 (300 mg, 0.56 mmol) was reacted with 1-amino-2-vinyl-cyclopropanecarboxylic acid ethyl ester (130 mg, 0.84 mmol) as described in Example 11 which gave the title compound (302 mg, 80 %).
Example 135
(Figure Removed)
1-({4-[2-(2~lsopropylamino-thiazol-4-yl)-7-methoxy-quinolin-4-yIoxy]-pyrrolidine-2-carbonyl}-amino)- 2-vinyl-cyelopropanecarboxylic acid ethyl ester (135) Compound 134 (302 mg, 0.45 mmol) was treated as described in Example 12 which gave the title compound (195 mg, 76%).
Example 136
(Figure Removed)
1 -({1-[1 -(2-Hydroxy-indan-1 -ylcarbamoyl)-2,2-dimethyl-propylcarbamoyl]-4-[2-(2-
isopropylamino-thiazol-4-yI)-7-methoxy-quinolin-4-y!oxy]-pyrroiidine-2-carbonyl}-
amino-2-vinyl-cycloprpoanecarboxylic acid ethyl ester (136)
Compound 135 (80 mg, 0.14 mmol) was treated as described in Example 13 which
gave the title product (87 mg, 72%).
Example 137
(Figure Removed)
1-({1-[1-(2-Hydroxyindan-1-yIcarbamoyl)-2,2-dimethyl-propylcarbamoyl]-4-[2-(2-
isopropylamino-thiazol-4-yl)-7-methoxy-quinolin-4-yloxy]-pyrrolidine-2-carbonyl}-
amino-2-vinyl-cycloprpoanecarboxylic acid (137)
The ethyl ester of'compound 136 (80 mg, 0.09 mmol) was hydrolyzed following the
procedure described in Example 14 which gave the title product Yield after
preparative LC-MS (7.5 mg, 10%).
Example 138
(Figure Removed)
1-{l1-Ethylcarbamoyl-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (138) Reaction of compound 12 (330 mg, 0.66 mmol), phosgene (1.6 ml, 1.9 M in toluene, 3 mmol) and hex-5-enylamine hydrochloride (500 mg, 3.68 mmol) following the procedure described in Example 120 gave the pure title product (328 mg, 80 %), MS (M+H*) 627.
Example 139
(Figure Removed)
tricyclo[13.3.0.0*4,6*]octadec-7-ene-4-carboxylic acid ethyl ester (139) Compound 138 (200 mg, mol) was dissolved in degassed dry dlchloromethane (200 ml), bubbled with nitrogen. Then Hoveyda-Grubbs (second generation) catalyst (5 mg, 2 mol %) was added and the reaction mixture was refluxed for 20 h under nitrogen. The resulting mixture was cooled down to room temperature and concentrated by rotary evaporation. The resulting oil was purified by column chromatography on YMC silica (ethyl acetate - toluene 1:1 to 9:1) to give 55 mg of the title compound as a beige solid. Yield 29%. MS (M+H+) 599.
Example 140
(Figure Removed)
-(T-Methoxy-Z-phenyl-quinolin--yloxy.-dioxo-S.IS.IS-triaza-tricyclot13.3.0.0*4,6*]octadec-7-ene-4-carboxylic acid (140) Compound 139 (55 mg, mol) was dissolved in 2 ml of methanol and mixed with 3 eq. of aqueous NaOH and heated for 2 h at 60 °C in a closed vial. The reaction mixture was then extracted into ethyl acetate. The water solution was collected and acidified with 1N HCI solution to pH 2. The resulting solution was concentrated by rotary evaporation, dissolved in methanol and purified by preparative HPLC (acetonitrile-water) to give 34 mg of the title product. Yield 65%. MS (M+H*) 571.
Example 141
(Figure Removed)
1-{[1-{1-[(Cyclohexyl-methoxycarbonyl-methyl)-carbamoyl]-2,2-dimethyI-propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-arnino}-2~vinyl-cyclopropanecarboxyiic acid (141).
Compound 103 was dissolved in dlchloromethane (3ml) and solid sodium bicarbonate (100 mg) and phosgene 20% in toluene (0.1 ml) was added. After 30 min at room temperature the mixture was concentrated to dryness. (S)-(2S-2-Amino-3,3-dimethyi-butyrylamino)-cyclQhexyl-acetic acid methyl ester (12 mg in dichloromethane 2 ml) was added. After Sdays of agitation at room temperature, the reaction mixture was filtered, concentrated to dryness and purified on preparative HPLC-MS which gave the title product (4.4 mg). M+H* 784.7.
Example 142
(Figure Removed)
1 -{[1 -0 -Aminomethyl-2,2-dimethyl-propylcarbamoyl-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid ethyl ester (142)
The title compound was prepared from compound 12 (1.22 g, 2.43 mmol) by following the procedure described for the preparation for compound 108 but using methanesulphonic acid 2-tert.butoxycarbonylamino-3,3-dimehtyl-butyI ester instead of methanesulphonic acid 2-tert.butoxycarbonylamino-4-mehtyl-pentyl ester, in Example 165 step i). Reduction of the azide as described in Example 109 gave the title compound (1.49 g, 95 %). Purity according to HPLC > 95%, M+H+ 644.2.
Example 143
(Figure Removed)

1-{[1-(2,2-Dimethy!-1-{[thiophene-3-carbonyl)-amino]-methyl}-propyIcarbamoyl)-4-(7-
methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-
cyclopropanecarboxylic acid (143)
Compound 142 (100 mg, 0,155 mmol) was reacted according to the general
procedure 1A for the preparation of compounds 110-116, using thiophene-3-carbony!
chloride (28.5 mg, 0.194 mmol) as acyl chloride which gave the title compound as a
white solid (45 mg, 40%). Purity according to HPLC > 95%, M+H+ 726.
Example 144
(Figure Removed)
1-{[1-{1-[(5-lsoxazol-3-yl-thiophene-2-sulphonylamino)-methyl]-2,2-dlnnethyl-propylcarbamoyl}-4-(7-methoxy-2-phenyl-quinolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylic acid (144)
Compound 142 (25 mg, 0.039 mmol) was reacted according to the general procedure 1A for the preparation of compounds 110-116, using 5-isoxazole-3-yl-thiophene-2-sulphonyl chloride (14,5 mg, 0.058 mmol) as acyl chloride which gave the title compound as a white solid (1.8 mg, 6%). Purity according to HPLC was > 94%, M+hT 829.
Example 145
(Figure Removed)
1-{[1-(3-Fluoro-benzoylamino)-methyl]-propylcarbamoyl}-4-(7-methoxy-2-phenyl-
•
qulnolin-4-yloxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxylicacid
(145)
Compound 142 (25 mg, 0.039 mmol) was reacted according to the general procedure
1A for the preparation of compounds 110-116, using 3-fluorobenzoyl chloride (12.3
mg, 0.078 mmol) as acyl chloride which gave the title compound as a white solid (4.1
mg, 14%). Purity according to HPLC was > 94%, M+H* 738.
Example 146
(Figure Removed)
1-{[1-(1{[(-Furan-3bcarbonyl)-amino]-methyl]-2,2-dimethyl-propylcarbamoyl}-4-(7-
methoxy-2-phenyl-quinolin-4-yIoxy)-pyrrolidine-2-carbonyl]-amino}-2-vinyI-
cyclopropanecarboxylic acid (146)
Compound 142 (25 mg, 0.039 mmol) was reacted according to the general procedure
1B for the preparation of compounds 110-116, using 3-furanoic acid (5.5 mg, 0.049
mmol) as acyl chloride which gave the title compound as a white solid (4.1 mg, 14%).
Purity according to HPLC was > 99%, M+H* 710.
Example 147
(Figure Removed)
4-(7-Methoxy-2-phenyl-quinolin-4-yloxy)-pyrroIidine-1,2-dicarboxylic acid 2-[(1-cyclopropanesulphonylaminocarbonyl-2-vinyl-cyclopropyl)-amide] 1-[(2,2-dimethyl-1-{[(thiophene-3-carbonyl)amino]-methyl}-propyl)-amide (147) To solution of compound 143 (42.2mg, 0.058mmol) in chloroform (3 ml) was added cyclopropylsulphonamide (14 mg, 0.116 rnmol) followed by diisopropylethylamine (60.5 pi, 0.17 mmol). The solution was stirred at RT for 10 min and then at -20°C for 30 min. PyBOP (121 mg, 0.116 mmol) was then added as a solid. The solution was kept at -20°C for 10 days. The solution was then poured into aqueous NaHCOs (sat.) and washed with water. The organic layer was dried, concentrated and subjected to purification by HPLC, affording the title compound as a white solid (2.3 mg, 0.0028 mmol), Purity by HPLC>95%, M+H+830.
Example 148
(Figure Removed)
Fmoc-4-amino-2-(1-ethoxycarbonyl-2-vinyl-cycIopropylcarbamoyl)-pyrrolidine-1-carbocyclic acid tert.butyl ester (148)
(2S,4R) Fmoc-4-amino-1-Boc-pyrrolidine-2-carboxylic acid (5.3 g, 11.8 mmol) was dissolved in DCM (100 ml), HATU (4.94 g, 12.99 mmol), DIEA (4.63 ml, 26.57 mmol) and vinylcyclopropylglycine ethyl ester (2.26 g, 11.81 mmol) were added
successively. The mixture was stirred for 16 h at room temperature, and was then diluted with DCM (50 ml), washed with citric acid (10% aq), water, NaHC03 (sat.aq) and water. The organic phase was dried over NaaSCU and concentrated to afford a beige solid foarn (8.11 g) which was subjected to silica gel column chromatography to afford the title compound (7.14 g, 12.11 mmol).
Example 149
(Figure Removed)
1-[(Fmoc-4-amino-pyrrol!dine-2-carbonyl)-amino]-2-vinyl-cyclopropanecarboxylicacid ethyl ester (149)
Compound 148 (3.65 g, 6.04 mmol) was treated with a solution of TFA/DCM (10ml TFA, 50ml DCM) for 2.5h and then concentrated to afford the titled compound (2.99 g, 6.12 mmol).
Example 150
(Figure Removed)
1-({Fmoc-4-amino-1-[1-(2-hydroxy-indan-1-ylcarbamoyl-2,2-dimethyl-propylcarbamoyl]-pyrrolidine-2-carbonyl}-amino)-2-vinyl-cycIopropanecarboxylicacid ethyl ester (150)
The amlnoproline derivative 149 (2.96 g, 6.04 mmol) was stirred together with phosgene (1.93 M in toluene, 4 ml, 7.55 mmol) for 10 min. The solvents and excess of phosgene were evaporated. The residue was dissolved in DCM (30 ml) and t-Bug-aminoindanol (1.9 g, 7.24 mmol) was added as a solution in DCM (30 ml), followed by NaHCOs (2 g). The mixture was stirred for 48h, then diluted with DCM, washed with
water, 10% citric acid and NaHCOa (sat, aq), dried over Na2SC>4, and evaporated to dryness. The residue was subjected to column chromatography purification, EtOAo hexane 0-30% to afford the title compound (1 g, 1.3 mmol).
Example 151
(Figure Removed)
1-({4-Amino-1-[1-(2-hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyl-propyIcarbamoyl]-pyrrolidine-2-carbonyl}-amino)-2-vinyl-cycloprpanecarboxylic acid ethyl ester (151) Compound 150 (595 mg, 0.765 mmol) was dissolve din DMF (20 ml) and treated with Si-piperazine (0.08 mmol/g, 4.78 g, 3.82 mmol) for48h. The silica was filtered and washed once with DMF and then with several portions of DCM. The solvents were evaporated and the residue subjected to column chromatography to afford the title compound (170 mg, 0.3 mmol).
Example 152
(Figure Removed)
1-({1-[1-(2-Hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyI-propylcarbamoyl]-4-[(pyridine-
3-carbonyl)-amino]pyrrolidine-2-carbonyl}-amino)-2-vinyl-cyclopropanecarboxy]ic
acid (152)
To a stirred solution of compound 151 (35 mg, 0.064 mmol) in DCM (1 ml), was
added DIEA (0.12 mmol, 19 (jl) and nicotinoyl chloride hydrochloride (0.12 mmol, 17
mg).The solution was stirred at RT for 18h, PS-trisamine was added then stirred at RT for 4h. After filtration, the solution was washed with citric acid (10% aq) and NaHCO3 (sat, aq), the organic phase was dried over NaaSC and concentrated. The residue was dissolved in THF:MeOH (2:1,1.5 ml). LiOH (1N aq, 3.2 mmol, 320 ul) was added. The solution was stirred at 60 DC for 24h. Acetic acid was added and then concentrated. The residue was dissolved in MeOH and subjected to purification by HPLC, affording the title compound (19.5 mg, 0.03 mmol). Purity by HPLO98%, M+H* 633.1.
Example 153
(Figure Removed)
1-({1-[1-(2-HydroxyMndan-1-ylcarbamoyl)-2,2-dimethyl-propylcarbamoyl]-4-
phenylacetamino-pyrrolidine-2-carbonyl}-amino)-2-vinyl-cyclopropanecarboxyIicacid
(153)
The procedure described in Example 152 but using phenyl acetyl chloride instead of
nicotinoyl chloride hydrochloride, was followed which gave the title compound (12.7
mg, 0.019 mmol). Purity by HPLO90%, M+H*646.1.
Example 154
(Figure Removed)
1-({1-[1-(2-Hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyI-propyIcarbamoyl]-4-[(5-methyl-
3-phenyl-isoxazole-4-carbonyl)-amino]-pyrrolidine-2-carbonyl}-amino)-2-vinyl-
cyclopropanecarboxylic acid (154)
The procedure described in Example 152 but using 5-methyl-3~phenyl-isoxazole-4-
carbonyl chloride instead of nicotinoyl chloride hydrochloride, was followed which
gave the title compound (3.6 mg, 00055 mmol). Purity by HPLC>98%, M+H*713.1.
Example 155
(Figure Removed)
1-{[1-[1-(2-Hydroxy-indan-1-ylcarbamoyl)-2l2-dimethyl-propylcarbamoyI]-4-(3-phenyl-ureido)-pyrrolidine2-carbonyl]-amino}-2-vinyl-cyclopropanecarboxyIic acid (155) To a stirred solution of compound 151 (30 mg, 0.054 mmol) in acetonitrile:dichloromethane (2:1, 3 ml), triethylamine (0.0648 mmol, 9 pi) and phenylisocyanate (0.0648 mmol, 7 ul) was added The solution was stirred at room temperature for 3h, methanol was added (1 ml) and then it was concentrated. The residue was dissolved in methanol and subjected to purification by HPLC, affording the ester compound as a white solid (32.7mg, 0,047 mmol), Purity by HPLC>95%, M+H* 675.31. LiOH 1N aq. (0.47mmol, 475 ul) was added to the ester dissolved in THF:MeOH (2:1). The reaction was stirred at 50 °C for 15 min and then at 8 °C for 12 h followed by addition of acetic acid (0.98 mmol, 53 pi) before concentration. The residue was dissolved In MeOH and subjected to purification by HPLC, affording the title compound as a white solid (3.8 mg, 0.006 mmol), Purity by HPLO98%, M+H+ 675.31.

(Figure Removed)
1-({4-Benzenesulphonylamino-1-[1-(2-hydroxy-indan-1-ylcarbamoyl)-2,2-dimethyl-propylcarbamoyI]-pyrrolidine-2-carbonyl}-amino)-2-vinyl-cycIopropanecarboxylicacid (156)
To a stirred solution of compound 151 (30 mg, 0.054 mmol) in DCM (2 ml), DIEA (0.0648 mmol, 11.5 pi) and phenysulfonylchloride (0.0648 mmol, 11.5 ul) were successively added .The solution was stirred at RT for 3h, and then it was concentrated. The residue was dissolved in MeOH and subjected to purification by HPLC, affording the ester compound as a white solid (17.9 mg, 0.0257 mmol), Purity by HPLO95%, M+H+ 696.24. LiOH 1N aq, (0.25 mmol, 257 pi) was added to the ester dissolved in THF:MeOH (2:1). The reaction was stirred at 50 °C for 1.5h prior to the addition of acetic acid (0.98 mmol, 53 ul). The solution was concentrated. The residue was dissolved in DCM and washed with water; the aqueous phase was acidified to pH 5 and then extracted with dichloromethane and ethyl acetate. The combined organic phases were dried over NaaSCvt and concentrated, affording the title compound as a white solid (7.1 mg, 0.01 mmol), Purity by HPLC>98%, M+H4 668.19.
Example 157
(Figure Removed)
1 -{[1 -[1 -(2-Hydroxy-indan-1 -ylcarbamoyl)-2,2-dimethyl-propylcarbamoyI]-4-(3-phenyl-thioureido)-pyrrolidine-2-carbonyl}-amino)-2-vinyl-cyclopropanecarboxylic acid (157) To a stirred solution of compound 151 (30 mg, 0.054 mmol) in acetonitril (3 ml), TEA (0.0648 mmol, 9 pi) and phenylthioisocyanate (0.0648 mmol, 7.8|jl) were successively added .The solution was stirred at RT for 16h, and then it was concentrated. The residue was dissolved in MeOH and subjected to purification by HPLC, affording the ester compound as a white solid (22 Jmg, 0.0328mmol), Purity by HPLO95%, M+H* 691.2. LiOH 1N aq, (0.33 mmol, 328 pi) was added to the ester dissolved in THF:MeOH (2:1). The reaction was stirred at 50 °C for 2.5h prior to the addition of acetic acid (0.98 rnmol, 53 pi). The solution was concentration. The residue was dissolved in dichloromethane and washed with water, the aqueous phase was extracted with EtOAc. The combined organic phases were dried over Na2S04 and concentrated, affording the title compound as a white solid (7.2 mg, 0.01 mmol), Purity by HPLC>98%, M+H+ 663.26.
Assays
The compounds of the invention are conveniently assayed for activity against the NS3 protease of flavivirus such as HCV using conventional in vitro (enzyme) assays or cell culture assays.
A useful assay is the Bartenshlager replicon assay disclosed in EP 1043399, An alternative replicon assay is described in WO 03064416.
A convenient enzyme assay involving the inhibition of full-length hepatitis C NS3 is essentially as described in Poliakov, 2002 Prot Expression & Purification 25 363 371. Briefly, the hydrolysis of a depsipeptide substrate, Ac-DED(Edans)EEAbu\|/[COO]ASK(Dabcyl)-NH2 (AnaSpec, San Jose, USA), is
measured spectrofluorometrically in the presence of a peptide cofactor, KKGSWIVGRIVLSGK, as described by Landro, 1997 Biochem 36 9340-9348. The enzyme (1 nM) is incubated in a buffer such as 50 mM HEPES, pH 7.5,10 mM DTT, 40% glycerol, 0.1% n-octyl-p-D-glucoside, with 25 p,M cofactor and inhibitor at say 30 °C for 10 min, whereupon the reaction is initiated by addition of substrate, typically 0.5 nM substrate. Inhibitors are typically dissolved in DMSO, sonicated for 30 s and vortexed. The solutions are generally stored at-20°C between measurements.
An alternative enzyme assay is described in WO 0399316 and employs an HCV NS3/4A protease complex FRET peptide assay. The purpose of this in vitro assay is to measure the inhibition of HCV NS3 protease complexes, derived from the BMS, H77C or J416S strains, as described below, by compounds of the present invention. This assay provides an indication of how effective compounds of the present invention would be in inhibiting HCV proteolytic activity.
Serum is taken from an HCV-infected patient. An engineered full-length cDNA template of the HCV genome (BMS strain) was constructed from DNA fragments obtained by reverse transcription-PCR (RT-PCR) of serum RNA and using primers selected on the basis of homology between other genotype la strains. From the determination of the entire genome sequence, a genotype I a was assigned to the HCV isolate according to the classification of Simmonds et al. (See P Simmonds, KA Rose, S Graham, SW Chan, F McOmish, BC Dow, EA Follett, PL Yap and H Marsden, J.Ciin. Microbiol., 31(6), 1493-1503 (1993)). The amino acid sequence of the nonstructural region, NS2-5B, was shown to be >97% identical to HCV genotype la (H77C) and 87% identical to genotype Ib (J4L6S). The infectious clones, H77C (I a genotype) and J4L6S (I b genotype) can be obtained from R. Puree!! (N!H) and the
sequences are published in Genbank (AAB67036, see Yanagi.M., Purcell.R.H., Emerson.S.U. and Bukh. Proc. Natl. Acad. Sci. U.S.A. 94 (16) 8738-8743 (1997); AF054247, see Yanagi.M., StClaire.M., Shapiro.M., Emerson.S.U., Purcell.R.H. and Bukhj, Virology 244 (1), 161 (1998)).
The BMS, H77C and J4L6S strains are conventional for for production of recombinant NS3/4A protease complexes. DNA encoding the recombinant HCV NS3/4A protease complex (amino acids 1027 to 1711) for these strains were manipulated as described by P. Gallinari et al. (see Gallinari P, Paolini C, Brennan D, Nardi C, Steinkuhler C, De Francesco R. Biochemistry. 38(17):562032, (1999)). Briefly, a three-lysine solubilizing tail was added at the 3'-end of the 3 0 NS4A coding region. The cysteine in the P1 position of the NS4A-NS4B cleavage site (amino acid 1711) was changed to a glycine to avoid the proteolytic cleavage of the lysine tag. Furthermore, a cysteine to serine mutation can be introduced by PCR at amino acid position 1454 to prevent the autolytic cleavage in the NS3 helicase domain. The variant DNA fragment can be cloned in the pET21b bacterial expression vector (Novagen) and the NS3/4A complex can be expressed in Escherichia coli strain BL21 (DE3) (Invitrogen) following the protocol described by P. Gallinari et al. (see Gallinari P, Brennan D, Nardi C, Brunetti M, Tomei L, Steinkuhler C, De Francesco R., J Viral. 72(8):6758-69 (1998)) with modifications. Briefly, NS3/4A expression can be induced with 0.5mM Isopropyl beta-D thiogalactopyranoside (IPTG) for 22hr at 20'C. A typical fermentation (10 I) yields approximately 80g of wet cell paste. The cells are resuspended in lysis buffer (IO mL/g) consisting of 25mM N-(2Hydroxyethyl)Piperazine-N'-(2-Ethane Sulfonic acid) (HEPES), pH7.5, 20% glycerol, 5OOmM Sodium Chloride (NaCI), 0.5% Triton-X100,1 ug/mL lysozyme, 5mM Magnesium Chloride (MgCI2), I ug/mL Dnasel, 5mM beta-Mercaptoethanol (BME), Protease inhibitor - Ethylenedfamine Tetraacetic acid (EDTA) free (Roche), homogenized and incubated for 20 mins at VC. The homogenate is sonicated and clarified by uitra-centrifugation at 235000 g for 1 hr at 4'C.
Imldazole is added to the supernatant to a final concentration of 15mM and the pH adjusted to 8. The crude protein extract is loaded on a Nickel Nitrilotriacetic acid (Ni-NTA) column pre-equilibrated with buffer B (25n-tM 2 0 HEPES, pH8 20% glycerol, 500mM NaCI, 0.5% Triton-XlOO, 15mM imidazole, 5mM BME). The sample is loaded at a flow rate of ImL/min. The column is washed with 15 column volumes of buffer C (same as buffer B except with 0.2% Triton-X100). The protein is eluted with 5 column volumes of buffer D (same as buffer C except with 200mM Imidazole).
NS3/4A protease complex-containing fractions are pooled and loaded on a desalting column Superdex-S200 pre-equilibrated with buffer D (25MM HEPES, pH7.5, 20% glycerol, SOOmM NaCI, 0.2% Triton-XlOO, lOmM BME). Sample is loaded at a flow rate of ImUmin. NS3/4A protease complexS 0 containing fractions are pooled and concentrated to approximately 0.5mg/mL The purity of the NS3/4A protease complexes, derived from the BMS, H77C and J4L6S strains, are typically judged to be greater than 90% by SDS-PAGE and mass spectrometry analyses.
The enzyme is generally stored at -80'C, thawed on ice and diluted prior to use in assay buffer. The substrate used for the NS3/4A protease assay, is conveniently RET . S 1 (Resonance Energy Transfer Depslpeptide Substrate; AnaSpec, Inc. cat # 22991 )(FRET peptide), described by Taliani et al. in Anal. Biochem. 240(2):6067 (1996). The sequence of this peptide is loosely based on the NS4A/NS4B natural cleavage site except there is an ester linkage rather than an amide bond at the cleavage site. The peptide substrate is incubated with one of the three recombinant NS3/4A complexes, in the absence or presence of a compound of the present invention, and the formation of fluorescent reaction product was followed in real time using a Cytofluor Series 4000. Useful reagents are as follow: HEPES and Glycerol (Ultrapure) can be obtained from GIBCO-BRL Dimethyl Sulfoxide (DMSO) is obtained from Sigma. Beta-Mercaptoethanol is obtained from Bio Rad,
Assay buffer: SOm.M HEPES, pH7.5; 0.15M NaCI; 0. l% Triton; 15 % Glycerol; 10mM BME. Substrate: 2 uM final concentration (from a 2mM stock 2 0 solution in DMSO
stored at -20'C). HCV NS3/4A type la (Ib), 2-3 nM final concentration (from a 5uM stock solution in 25mM HEPES, pH7.5, 20% glycerol, 300m.M NaCI, 0.2% Triton-X100,10mM BME). For compounds with potencies approaching the assay limit, the assay can be made more sensitive by adding 50 ug/mL BSA to the assay buffer and/or reducing the end protease concentration to 300 pM.
The assay is conveniently performed in a 96-well polystyrene black plate from Falcon. Each well contains 25ul NS3/4A protease complex in assay buffer, 50ul of a compound of the present invention in 10% DMSO/assay buffer and 25ul substrate in assay buffer. A control (no compound) is also prepared on the same assay plate. The enzyme complex is mixed with compound or control solution, typically for 1 min before initiating the enzymatic reaction by the addition of substrate. The assay plate is generally read immediately using a spectrophotometer such as a Cytofluor Series 4000 (Perspective Biosysterns), The instrument is conveniently set to read an emission of 340nm and excitation of 490nm at 25'C. Reactions are generally followed for approximately 15 minutes.
The percent inhibition can be calculated with the following equation.
100.-[(dF,nh/dF00n)XIOO]
where dF is the change in fluorescence over the linear range of the curve. A nonlinear curve fit is applied to the inhibition-concentration data, and the 50% effective concentration (IC50) is calculated by the use software such as Excel Xl-fit software using the equation:
y=A+((B-A)/(1+((C/x)AD))).
Enzyme assays conveniently utilize a fluorescence resonance energy transfer (FRET) principle to generate a spectroscopic response to an HCV NS3 serine protease catalyzed NS4A/4B cleavage event. The activity is typically measured in a continuous fluorometric assay using an excitation wavelength of 355 nm and emission wavelength of 500 nm. The initial velocity may be determined from 10 minutes
continuous reading of increased fluorescence intensities as a result of the NS3 protease catalyzed cleavage event.
An alternative enzyme assay can be carried out as follows:
Materials
Recombinant HCV NS3 full length enzyme can be prepared as shown in Poliakov et
al Protein Expression & purification 25 (2002) 363-371.
The NS4A cofactor conveniently has an amino acid sequence of
KKGSWIVGRIVLSGK (commercially available), generally prepared as a 10 mM
stock solution in DMSO.
TheFRET-substrate(Ac-Asp-Glu-Asp(EDANS)-Glu-GIu-Abu-HJ-[COO)Ala-Ser-
Lys(DABCYL)-NH2, MW1548.60 can be purchased from AnaSpec RET S1, CA.
USA) and is typically prepared as a 1.61 mM stock solution in DMSO. Aliquots
(50ul/tube) should be wrapped with aluminum foil to protect from direct light and
stored in -20 °C.
Reference compound-1, N-1725 with a sequence of AcAsp-D-Gla-Leu-ile-Cha-Cys,
MW 830.95 may be purchased from BACHEM, Switzerland and is generally prepare
as a 2 mM stock solution in DMSO and stored in aliquots in -20°C.
1M HEPES buffer may be purchased from Invitrogen Corporation, storage at 20°C.
Glycerol may be purchased from Sigma, 99% purity.
CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate: may be
purchased from Research Organics, Cleveland, OH44125, USA. MW614.90
DTT, DL-Dithiothreitol (Cleland Reagent: DL-DTT) 99% purity, MW.154.2 Storage:
+4°C.
DMSO may be purchased from SDS, 13124 Peypin, France. 99.5% purity.
TRIS, ultra pure (TRIS-(hydroxymethylaminomethane), may be purchased from ICN
Biomedicals Inc.
N-dodecyI-|3-D-maltoside, minimum 98%, may be purchased from Sigma, storage -
20°C.
Equipment
Microtiter plates (white cliniplate, ThermoLab Systems cat no. 9502890)
Eppendorf pipettes.
Biohit pipette, multi dosing.
Ascent fluorimeter, filterpair ex 355nm, emSOO nm.
Method
Experimental procedure:
10 mM stock solutions of the compounds are made in DMSO. The stock solutions are stored in room temperature while testing and placed in -20°C at long-time storage. Assay buffer A:
50 mM HEPES buffer, pH=7.5 ,40% Glycerol, 0.1% CHAPS
Storage: room temperature
10 mM DTT (stored in aliquots at -20°C and added fresh at each experiment) Assay buffer B:
25 mM TRIS pH7.5, 0.15 M NaCI, 10% glycerol, 0.05% n-dodecyl-p-D-
maltoside
5mM DTT (stored in aliquots at -20°C and added fresh at each experiment) Experiment sequence: Preparation of reaction buffer (for one Plate. 100 reactionsMbuffer A)
Prepare 9500pl assay buffer (HEPES, pH=7.5, 40% glycerol and 0.1% CHAPS
in de ionized water. Add DTT giving a final concentration of 10mM (freshly
prepared for every run).
Thaw rapidly the NS3 protease
Add 13.6 Ml NS3 protease and 13.6 ul NS4A peptide and mix properly. Leave
the mixture for 15 minutes in room temperature.
Place the enzyme stock solution back into liquid nitrogen or -80°C. as soon as
possible.
Preparation of reaction buffer (for one plate, 100 reactionsKbuffer B)
Prepare 9500 Ml assay buffer (TRIS, pH=7.5, 0.15 M NaCI, 0.5 mM EDTA,
10% glycerol and 0.05% n-dodecyl (3-D-maltoside in de ionized water. Add
DTT giving a final concentration of 5mM (freshly prepared for every run).
Thaw the NS3 protease rapidly.
Add 27.2 pi NS3 protease and 13.6 pi NS4A peptide and mix properly. Leave
the mixture for 15 minutes in room temperature.
Place the enzyme stock solution back into liquid nitrogen or -8Q°C as soon as
possible.
Preparation of inhibitor/reference compound
Make a dilution series of the Inhibitors in DMSO to 100xthe final concentrations 10,1, 0.1, 0.01 and 0.001 M. The final DMSO concentration in lOOul total reaction volume is1%. Make a dilution series of the reference compound, N-1725 in DMSO to 100x the final
*
concentrations 120, 60, 30,15, 7.5 and 3.75 nM.
Eight enzyme control wells are needed for every run.
Blank wells contain 95uL buffer (without NS3 PR), 1uL DMSO and 5 uL substrate.
Preparation of FRET substrate
Dilute the substrate stock solution (1.61 mM) with assay buffer to 40 uM working
solution. Avoid exposure to light.
Assay sequence
Use 96-well cliniplate, the total assay volume per well is 100 ul.
Add 95uL of assay buffer to each well
Add 1 ul inhibitor/reference compound
Pre incubate for 30 minutes at room temperature
Start the reaction by adding 5uL 40 uM substrate solution (final concentration
2uM)
Read continuously for 20 minutes at ex=355nm and em=500nm, monitoring the
increased fluorescence per minute.
Plot the progression curve (within linear range, 8-10 time points) and determine
the slope as an initial velocity with respect to each individual inhibitor
concentration.
Calculate %inhibition with respect to enzyme control.

Treatment of Results
The result is expressed as %inhibition at a certain concentration (screen) or as a Ki
value in nM or |JM.
Calculation of % inhibition.
The initial velocity is determined from 10 minutes continuous reading of increased
fluorescence intensities as a result of the NS3 protease catalyzed cleavage event.
The change in slope for the inhibitor compared to the enzyme control gives the %
inhibition at a certain concentration.
Calculation of Ki.
All inhibitors are treated as if they follow the rules of competitive inhibition.
The ICso value is calculated from the inhibition values of a series of inhibitor
concentrations. The calculated value is used in the following equation:
Ki=IC50/(1+S/Km)
Plotting of the graph is done by help of two calculation programs: Grafit and
Graphpad
Various compounds of the invention exemplified above displayed ICeos in the range 1 nM to 6.9 micromolar and ED5os in the sub-micromolar to micromolar range.
Drug escape resistance pattern and rate
Replicon cultures in microtitre plates can be used to determine resistance development rates and to select out drug escape mutants. The compounds being tested are added at concentrations around their EDso using, say, 8 duplicates per concentration. After the appropriate replicon incubation period the protease activity in the supernatant or lysed cells is measured.
The following procedure is followed at subsequent passages of the cultures, Virus produced at the concentration of test compound showing > 50% of the protease activity of untreated infected cells (SIC, Starting Inhibitory Concentration) are passaged to fresh replicon cultures. An aliquot, say, 15jj,l supernatent from each of
the eight duplicates are transferred to replicon cells without the test compound (control) and to cells with test compound at the same concentration, and additionally two respectively fivefold higher concentrations. (See the table below below)
When the viral component of replicon propagation (for example as measured by HCV
protease activity) is permitted at the highest non-toxic concentration (5 - 40 \M),
2-4 parallel wells are collected and expanded to give material for sequence analysis
and cross-wise resistance.
Key:
Viral growth permitted
Virus production inhibited
(Table Removed)
Alternative methods for assessing activity on drug escape mutants include the preparation of mutant enzyme bearing the distinctive mutation for use in standard Ki determinations as shown above. For example WO 04/039970 describes constructions allowing access to HCV proteases bearing the 155,156 and/or 168 drug escape mutants arising from the selective pressure of BILN-2061 and VX-950. Susch constructs can then be engineered into replicon vectors in place of the wild type protease, thereby allowing ready assessment in a cellular assay, of whether a given compound is active against a give drug escape mutant.
P450 metabolism
The metabolism of compounds of the invention through the main isoforms of the human cytochrome system P450 are conveniently determined in baculovirus infected insect cells transfected with human cytochrome P450 cDNA (supersomes) Gentest Corp. Woburn USA.
The test compounds at concentrations 0.5,5 and 50 \iM are incubated in duplicate in
*
the presence of supersomes ovenexpressing various cytochrome P450 isoforms, including CYP1A2 + P450 reductase, CYP2A6 + P450 reductase, CYP2C9-Arg 144 + P450 reductase, CYP2C19 + P450 reductase, CYP2D6-Val 374 + P450 reductase and CYP3A4 + P 450 reductase. Incubates contain a fixed concentration of cytochrome P450 (eg 50 pmoles) and are conducted over 1 hour. The involvement of a given isoform in the metabolism of the test compound Is determined by UV HPLC chromatographically measuring the disappearance of parent compound.





Claims
1. Macrocyclic proline analogues of the formula I:

(Formula Removed)
wherein
A is C(=O)R1, or C(=O)NHSO2R2, wherein;
R1 is hydrogen, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl; R2 is C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl; wherein
R2 is optionally substituted with 1 to 3 substituents independently selected from the group consisting of halo, oxo, nitrile, azido, nitro, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, NH2CO-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb, Y-(C=0)NRaRb, Y-NRaC(=O)Rb, Y-NHSOpRb, Y-S(=O)pRb and Y-S(=O)pNRaRb, Y-C(=O)ORb, Y-NRaC(=O)ORb;
Y is independently a bond or C1-C3alkylene;
Ra is independently H or C1-C3alkyl;
Rb is independently H, C1-C6alkyl, C0-C3alkylcarbocyclyl or C0-
C3alkylheterocyclyl;
p is independently 1 or 2;
M is CR7R7';
R7 is C1-C6alkyl, C0-C3alkylC3-C7cycloalkyl, or C2-C6alkenyl, any of which is
optionally substituted with 1-3 halo atoms, or an amino, -SH, or C0-
C3alkylcycloalkyl group; or
R7 is J;
R7 is H or taken together with R7 forms a C3-C6cycloalkyl ring optionally
substituted with R7a wherein;
R7a is C1-C6alkyl, C3-C5cycloalkyl, C2-C6alkenyl any of which may be optionally substituted with halo; or R7a can be J; q is 0 to 3 and k is 0 to 3; where q+k ≥ 1;
W is -CH2-, -O-, -OC(=O)NH-, -OC(=O)-, -S-, -NH-, -NRa, -NHSO2-, -NHC(=O)NH- or -NHC(=O)-, -NHC(=S)NH- or a bond; R8 is a ring system containing 1 or 2 saturated, partially saturated or unsaturated rings each of which has 4-7 ring atoms and each of which has 0 to 4 hetero atoms independently selected from S, O and N, the ring system being optionally spaced from W by a C1-C3alkylene group; any of which R8 groups can be optionally mono-, di-, or tri-substituted with R9, wherein
R9 is independently selected from the group consisting of halo, oxo, nitrile, azido, nitro, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, NH2C(=O)-, Y-NRa'Rb, Y-O-Rb, Y-C(=O)Rb, Y-(C=O)NRa'Rb, Y-NRaC(=O)Rb, Y-NHSOpRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-C(=O)ORb, Y-NRaC(=O)ORb; wherein Ra' is Ra with the proviso that when W is -S- or -O-, R8 is C0-C3alkylaryl or C0-C3alkylheteroaryl, Y is a bond and Rb is H or C1-C6 alkyl, then Ra' is Ra or C1-C6 alkyl and; wherein said carbocyclyl or heterocyclyl is optionally substituted with R10; wherein
R10 is C1-C6alkyl, C3-C7cycloalkyl, C1-C6alkoxy, amino, amido, sulfonyl, (C1-C3 alkyl)sulfonyl, NO2, OH, SH, halo, haloalkyl, carboxyl; E is -C(=O)-;
X is -NRx- where Rx is H, C1-C5alkyl or J; or in the case where E is -C(=O), X can also be -O- or -NRjNRj-;
wherein one of Rj is H and the other is H, C1-C5 alkyl or J; R11 is H, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, any of which can be substituted with halo, oxo, nitrile, azido, nitro, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, NH2C(=O)-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb, Y-(C=0)NRaRb, Y-NRaC(=O)Rb, Y-NHSOpRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-C(=O)ORb, Y-NRaC(=O)ORb; or R11 is J; J, if present, is a single 3 to 10-membered saturated or partially unsaturated alkylene chain extending from the R7/R7'cycloalkyl or from the carbon atom to which R7 is attached to one of Rj, Rx, Ry or R11 to form a macrocycle, which
chain is optionally interrupted by one to three heteroatoms independently selected from: -0-, -S- or -NR12-, and wherein 0 to 3 carbon atoms in the chain are optionally substituted with R14; wherein;
R12 is H, C1-C6alkyl, C3-C6cycloalkyl, or C(=O)R13;
R13 is C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl;
R14is independently selected from the group consisting of H, C1-C6alkyl,
C1-C6haloalkyl, C1-C6alkoxy, hydroxy, halo, amino, oxo, thio and C1-
C6thioalkyl; Ru is independently H or C1-C3alkyl; m is 0 or 1; n is 0 or 1; U is =0 or is absent;
R15 is H, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, any of which can be substituted with halo, oxo, nitrile, azido, nitro, C1-C6 alkyl, C0-C3alkylheterocyclyl, C0-C3alkylcarbocyclyl, NH2CO-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb, Y-(C=O)NRaRb, Y-NRaC(=O)Rb, Y-NHS0pRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-C(=O)ORb, Y-NRaC(=O)ORb;
G is -0-, -NRy-, -NRjNRj-: where one Rj is H and the other Rj is H, C1-C5 alkyl or J;
Ry is H, C1-C3 alkyl; or Ry is J;
R16 is H; or C1-C6 alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, any of which can be substituted with halo, oxo, nitrile, azido, nitro, C1-C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylheterocyclyl, NH2CO-, Y-NRaRb, Y-O-Rb, Y-C(=O)Rb, Y-(C=O)NRaRb, Y-NRaC(=O)Rb, Y-NHSOpRb, Y-S(=O)pRb, Y-S(=O)pNRaRb, Y-C(=O)ORb, Y-NRaC(=O)ORb;
with the proviso that when m=n=0 and G is O then R16 is not tert.butyl or phenyl;
wherein any C atom in C1-C6alkyl and C1-C3alkyl, unless otherwise specified, may optionally be substituted by one, two, or where valency permits three halogens;
each aryl and cycloalkyl moiety in C0-C3 alkylaryl and C0-C3-alkylC3-C7cycloalkyl, unless otherwise specified is optionally substituted with 1-3 substituents selected from halo, hydroxyl, nitro, cyano, carboxy, C1-C6alkyl, C1-C6alkoxy, C1-C6alkoxyC1-C6alkyl, C1-C6alkaonoyl, amino, azido, oxo, mercapto, C0-C3alkylcarbocylyl, and C0-C3alkylheterocyclyl; and
each amido group is selected from C(=O)NH2, C(=O)NHC1-C6alkyl, C(=O)N(C1-C6alkyl)2 and -NHC(=O)C1-C6alkyl; or a pharmaceutically acceptable salt thereof.
2. A compound as claimed in 1, wherein R7 is H and R7 is n-ethyl,
cyclopropylmethyl, cyclopropyl, cyclobutylmethyl cyclobutyl or
mercaptomethyl, preferably n-propyl or 2,2-difluoroethyl.
3. A compound as claimed in 1, wherein R7 and R7 together define a
spiro-cyclopropyl or spiro-cyclobutyl ring, both optionally mono or di-
substituted with R7'a wherein;
R7a is C1-C6 alkyl, C3-C5cycloalkyl, or C2-C6 alkenyl, any of which is optionally substituted with halo; or R7a is J.
4. A compound as claimed in claim 3, wherein the ring is a spiro-
cyclopropyl ring substituted with R7,a wherein;
R7a is ethyl, vinyl, cyclopropyl, 1- or 2-bromoethyl, 1-or 2-fluoroethyl, 2-bromovinyl or 2-fluorethyl.
5. A compound as claimed in claim 1, wherein R7 is J and R7 is H.
6. A compound as claimed in any one of claims 1-5 with the partial
structure la:
(Structure Removed)
7. A compound as claimed in claim 1, wherein m is 0 and n is 0.
8. A compound as claimed in claim 7, wherein G is -NRy- or -NRjNRj-.
9. A compound as claimed in claim 8, where Ry or one of the Rj groups is J, thereby defining a macrocyclic compound.
10. A compound as claimed in any claim 9, wherein R16 is H, C1-C3alkyl or C3-C6cycloalkyl.
11. A compound as claimed in claim 1, wherein m is 1.
12. A compound as claimed in claim 11, wherein X is -NRx-.
13. A compound as claimed in claim 11, wherein U is O.
14. A compound as claimed in any one of claims 14, wherein R11 is Cr C6alkyl, C0-C3alkylcarbocyclyl, C0-C3alkylaryl or C0-C3alkylheteroaryl, any of which is optionally substituted with halo, amino, C1-C6alkoxy, C1-C6thioalkyl, carboxyl, (C1-C6alkoxy)carbonyl, aryl, heteroaryl or heterocyclyl, and especially wherein the substituent is hydroxy or C(=O)OR14.
15. A compound as claimed in claim 14, wherein R11 is phenylethyl, 2,2-dimethyl-propyl, cyclohexylmethyl, phenylmethyl, 2-pyridylmethyl, 4-hydroxy-phenylmethyl, or carboxylpropyl; or especially tert-butyl, iso-butyl, or cyclohexyl.
16. A compound as claimed in claim 11, wherein one of Rx or R11 is J, thereby defining a macrocyclic compound.
17. A compound as claimed in claims 11, wherein n is 1.
18. A compound as claimed in claim 17, wherein R15 is C1-C6alkyl or C0-C3alkylcarbocyclyl, either of which is optionally substituted.
19. A compound as claimed in claim 18, wherein R15 is cyclohexyl, cyclohexylmethyl, tert-butyl, iso-propyl, or iso-butyl.
20. A compound as claimed in claim 11, wherein G is NRy or -NRjNRj-, where Ry or one Rj is H or methyl, and the other Rj is H.
21. A compound as claimed in claim 20, wherein R16 is H, C1-C6alkyl, or a 5 or 6 membered heterocycle, especially morpholine, piperidine or piperazine.
22. A compound as claimed in claim 11, wherein R16 is C1-C6alkyl, C0-C3alkylheterocyclyl, C0-C3alkylcarbocyclyl, any of which is optionally substituted with hydroxy, halo, amino, or C1-C6alkoxy.
23. A compound as claimed in claim 22, wherein R16 is 2-indanol, indanyl, 2-hydroxy-1-phenyl-ethyl, 2-thiophenemethyl, cyclohexylmethyl, 2,3-methylenedioxybenzyl, cyclohexyl, benzyl, 2-pyridylmethyl, cyclobutyl, iso-butyl, n-propyl, or 4-methoxyphenylethyl.
24. A compound as claimed in claim 1, wherein W is -OC(=O)-, -NRa-, -NHS(O)2- or -NHC(=O)-; or especially -OC(=O)NH- or -NH.
25. A compound as claimed in claim 1, wherein W is -S-, a bond or especially -O-.
26. A compound as claimed in claim 24 or 25, wherein R8 is optionally substituted C0-C3alkylcarbocyclyl or optionally substituted C0-C3-alkylheterocyclyl.
27. A compound as claimed in claim 26, wherein the C0-C3alkyl moiety is methylene or preferably a bond.
28. A compound as claimed in claim 27, wherein R8 is C0-C3alkylaryl, or
C0-C3alkylheteroaryl, either of which is optionally mono, di, or tri substituted
with R9, wherein;
R9 is C1-C6 alkyl, C1-C6alkoxy, NO2, OH, halo, trifluoromethyl, amino or amido (optionally mono- or di-substituted with C1-C6alkyl), C0-C3alkylaryl, C0-C3alkylheteroaryl, carboxyl, the aryl or heteroaryl being optionally substituted with R10; wherein
R10 is C1-C6alkyl, C3-C7cycloalkyl, C1-C6alkoxy, amino optionally mono- or di-substituted with C1-C6alkyl, amido, sulfonylC1-C3alkyl, NO2, OH, halo, trifluoromethyl, carboxyl, or heteroaryl.
29. A compound as claimed in claim 28, wherein R9 is C1-C6alkyl, C1-
C6alkoxy, amino, di-(C1-C3 alkyl)amino, C1-C3alkylamide, aryl or heteroaryl,
the aryl or heteroaryl being optionally substituted with R10; wherein
R10 is C1-C6alkyl, C3-C7cycloalkyl, C1-C6alkoxy, amino, mono- or di-C1-C3 alkylamino, amido, halo, trifluoromethyl, or heteroaryl.
30. A compound as claimed in claim 29, wherein R10 is C1-C6alkyl, C1-C6alkoxy, amino optionally mono- or di substituted with C1-C3 alkyl, amido, C1-C3-alkylamide, halo or heteroaryl.
31. A compound as claimed in claim 30 wherein R10 is methyl, ethyl, isopropyl, tert-butyl, methoxy, chloro, amino optionally mono- or di substituted with C1-C3 alkyl, amido or C1-C3alkyl thiazolyl.
32. A compound as claimed in claim 31, wherein R8 is 1-naphthylmethyl, 2-naphthylmethyl, benzyl, 1-naphthyl, 2-naphthyl, or quinolinyl any of which is unsubstituted, mono, or disubstituted with R9 as defined.
33. A compound as claimed in claim 31 wherein R8 is 1-naphthylmethyl, or quinolinyl any of which is unsubstituted, mono, or disubstituted with R9 as defined.
34. A compound as claimed in claim 33 wherein R8 is:
(Formula Removed)
wherein R9a is C1-C6 alkyl; C1-C6alkoxy; thioC1-C3alkyl; amino optionally substituted with C1-C6alkyl; C0-C3alkylaryl; or C0-C3alkylheteroaryl, C0-C3alkylheterocyclyl, said aryl, heteroaryl or heterocycle being optionally substituted with R10 wherein
R10 is C1-C6alkyl, C0-C3alkylC3-C7cycloalkyl, C1-C6alkoxy, amino optionally mono- or di-substituted with Ci-CealkyI, amido, C1-C3alkyl amide; and R9b is C1-C6 alkyl, C1-C6-alkoxy, amino, di(C1-C3alkyl)amino, (C1-C3alkyl) amide, N02, OH, halo, trifluoromethyl, carboxyl.
35. A compound as claimed in claim 34, wherein R9a is aryl or heteroaryl,
either of which is optionally substituted with R10 as defined.
36. A compound as claimed in 35, wherein R9a is selected from the group
consisting of:
(Formula Removed)
wherein R10 is H, C1-C6alkyl, or C0-C3alkylcycloalkyl, amino optionally mono-or di-substituted with C1-C6alkyI, amido, (C1-C3alkyl)amide.
37. A compound as claimed in claim 35, wherein R9a is optionally substituted phenyl, preferably phenyl substituted with C1-C6alkyl; C1-C6alkoxy; or halo.
38. A compound as claimed in claim 34, wherein R8 is:
(Formula Removed)
wherein R10a is H, C1-C6alkyl, or C0-C3alkylcarbocyclyl, amino optionally mono- or di-substituted with C1-C6alkyl, amido, heteroaryl or heterocyclyl; and R9b is C1-C6 alkyl, C1-C6-alkoxy, amino, di(C1-C3 alkyl)amino, amido, NO2, OH, halo, trifluoromethyl, or carboxyl.
39. A compound as claimed in claim 34, wherein R9b is C1-C6-alkoxy, preferably methoxy.
40. A compound as claimed in claim 1, wherein A is C(=O)NHSO2R2.
41. A compound as claimed in claim 40, wherein R2 is optionally substituted C1-C6alkyl, preferably methyl.
42. A compound as claimed in claim 40, wherein R2 is optionally substituted C3-C7cycloalkyl, preferably cyclopropyl.
43. A compound according to claim 40, wherein R2 is optionally substituted C0-C6alkylaryl preferably optionally substituted phenyl.
44. A compound as claimed in claim 1, wherein A is C(=O)OR1.
45. A compound as claimed in claim 44, wherein R1 is H or C1-C6alkyl, preferably hydrogen, methyl, ethyl, or tert-butyl.
46. A compound as claimed in claim 1, wherein J is a 3 to 8-membered saturated or unsaturated alkylene chain optionally containing one to two heteroatoms independently selected from: -O-, -S- or -NR12- , wherein R12 is H, C1-C6 alkyl, such as methyl, or -C(=O)CrC6 alkyl, such as acetyl.
47. A compound as claimed in claim 46, wherein J is a 4 to 7-membered saturated or unsaturated, all carbon alkylene chain.
48. A compound as claimed in claim 46, wherein J is saturated or mono-unsaturated.
49. A compound as claimed in claim 46, wherein J is dimensioned to provide a macrocycle of 14 or 15 ring atoms.
50. A compound as claimed in claim 1 with the formula the
(Formula Removed)
51. A compound as claimed in claim 50, wherein J is a 6 or 7 chain atom
saturated or partially unsaturated alkylene chain.
52. A compound as claimed in any one of claims 50-51, wherein J has one
unsaturation.
53. A compound as claimed in any of claims 50-52, wherein W is O and R8
is aryl or heteroaryl, either of which is optionally mono, di or tri substituted with
R9, wherein R9 is C1-C6 alkyl, C1-C6alkoxy, NO2, OH, halo, trifluoromethyl,
amino, amido, C0-C3alkylaryl, Co-C3alkylheteroaryl or carboxyl, wherein the
aryl or heteroaryl moiety Is optionally substituted with R10; wherein
R10 is C1-C6alkyl, C3-C7cycloalkyl, C1-C6alkoxy, amino, amido, sulfonylC1-C3alkyl, NO2, OH, halo, trifluoromethyl, carboxyl or heteroaryl.
54. A compound as claimed in claim 53, wherein R9 is selected from C1-
C6alkyl, C1-C6alkoxy, amino, amido, aryl or heteroaryl, wherein the aryl or
heteroaryl is optionally substituted with R10; wherein
R10 is C1-C6alkyl, C3-C7cycloalkyl, C1-C6alkoxy, amino, amido, halo, trifluoromethyl or heteroaryl.
55. A compound as claimed in any one of claims 50-54, wherein R8 is
(Formula Removed)
wherein R9a is C1-C6alkyl; C1-Cealkoxy; thioC1-C3alkyl; amino optionally
substituted with C1-C6 alkyl; C0-C3alkylaryl; C0-C3alkylheteroaryl or C0-
C3alkylheterocyclyl; said aryl, heteroaryl or heterocyclyl being optionally
substituted with R10;
R10 is C1-C6alkyl, C3-C7cycloalkyl, C1-C6alkoxy, amino, amido, heteroaryl or
heterocyclyl; and
R9b is C1-C6alkyl, C1-C6alkoxy, amino, amido, NO2, OH, halo, trifluoromethyl
or carboxyl.
56. A compound as claimed in claim 55, wherein R9a is
(Formula Removed)
wherein R10 is H, C1-C6alkyl, C3-C6cycloalkyl, amino optionally mono or disubstituted with C1-C6alkyl, amido, heteroaryl or heterocyclyl.
57. A compound as claimed in any one of claims 51-56, wherein A is
C(=O)NHSO2R2
58. A compound as claimed in any one claims 57, wherein R2 is methyl,
cyclopropyl or optionally substituted phenyl.
59. A compound as claimed in any one of the preceding claims for manufacture of a medicament for the prophylaxis or treatment of flavivirus infections, including HCV wherein said medicament optionally comprises an additional HCV antiviral, selected from nucleoside analogue polymerase inhibitors, protease inhibitors, ribavirin and interferon .

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Patent Number 252483
Indian Patent Application Number 3854/DELNP/2006
PG Journal Number 21/2012
Publication Date 25-May-2012
Grant Date 17-May-2012
Date of Filing 05-Jul-2006
Name of Patentee MEDIVIR AB
Applicant Address LUNASTIGEN 7, S-141 44 HUDDINGE, SWEDEN.
Inventors:
# Inventor's Name Inventor's Address
1 ASA ROSENQUIST MEDIVIR AB, LUNASTIGEN 7, S-141 HUDDINGE, SWEDEN
2 SUSANA AYESA C/O MEDIVIR AB, LUNASTIGEN 7, S-141 44 HUDDINGE, SWEDEN
3 LAZLO RAKOS C/O MEDIVIR AB, LUNASTIGEN 7, S-141 44 HUDDINGE, SWEDEN
4 BERTIL SAMUELSSON C/O MEDIVIR AB, LUNASTIGEN 7, S-141 44 HUDDINGE, SWEDEN.
5 FREDRIK THORSTENSSON C/O IFM FACULTY, UNIVERSITY OF LINKOPING, S-581 83 LINKOPING, SWEDEN
6 PER-OLA JOHANSSON C/O IFM FACULTY, UNIVERSITY OF LINKOPING, S-581 83 LINKOPING, SWEDEN
7 INGEMAR KVARNSTROM C/I IFM FACULTY, UNIVERSITY OF LINKOPING, S-581 83 LINKOPING, SWEDEN
8 BJORN CLASSON C/O MEDIVIR AB, LUNASTIGEN 7, S-141 44 HUDDINGE, SWEDEN
PCT International Classification Number C07K 5/02
PCT International Application Number PCT/SE05/000096
PCT International Filing date 2005-01-28
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 0400199.6 2004-01-30 Sweden
2 0401288-6 2004-05-19 Sweden
3 0402562-3 2004-10-22 Sweden