| Title of Invention | "A PROCESS OF TESTING UREA IN MILK" |
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| Abstract | A process of testing urea in milk comprising of, a filter paper, milk and indicator solution, putting a drop of indicator solution on a filter paper, an equal amount of milk to be tested is put on the indicator solution drop, characterized by the said indicator solution comprising a mixture of solution A and B, the said solution A is obtained by dissolving 1.6-1.8% P-Dimethylamino Benzaldehyde in dehydrated alcohol such as ethanol followed by adding 9-11% of Hydrochloric acid, the said solution B is obtained by dissolving 0.6-0.7% of Methyl Red and 0.3 -0.4% Bromo cresol green in a solvent as herein described, solution B is added in solution A in an amount of 0.9 to 1.1% to obtain the indicator. |
| Full Text | FILED OF INVENTION This invention relates to a process of testing urea in milk and an indicator solution thereof. PRIOR ART Milk is one of the important food articles liable to be adulterated for several factors. The problem of adulteration of milk with synthetic milk has increased recently. Generally, the synthetic milk consists of vegetable fat (refined palmolein oil preferred), detergent and urea mixed in water containing salt. Sugar, white paste, white paint, sodium bicarbonate and formaldehyde have also been detected in synthetic milk. This milk is similar to natural milk in colour, fat content, smell, taste and specific gravity but it has less cost of production than real milk. The synethetic milk is harmful to human being as it damages liver, heart and kidney. It also causes swelling of limbs and impaired vision. Some deaths have also been reported due to consumption of the synthetic milk. Its effects are more serious for children for whom milk forms the major part of their diet. It acts as a slow poison for them. Urea is one of the important ingredients used for making synthetic milk. Usually 0.9 to 1.0% urea is added for the manufacture of synthetic milk to make up its protein content. Normal milk also contains urea in the range of 0.02 to 0.07%. Presence of urea over and above this cut off value of 0.07% is considered as an adulternation with synethetic milk containing urea. Therefore, there is a need for rapid tests for the detection of urea in the milk to prevent the adverse health hazards to common man particularly children. Spectophotometric analysis for testing of urea in milk known in the art has certain disadvantages. A disadvantage is that it is time consuming. Another disadvantage of the above method is that milk sample needs to be taken to a laboratory and as such this test cannot be carried out in the field or at the home of consumers/farmers for on the spot testing of urea. - OBJECTS OF PRESENT INVENTION An object of the present invention is to propose a process of testing urea in milk and an indicator solution thereof. Another object of the present invention is to propose a process of testing urea in milk and an indicator solution thereof, which enables rapid testing of urea in milk within a minute time. Still another object of the present invention is to propose a process of testing urea in milk and an indicator solution thereof which enables on the spot testing of urea in milk at home by consumers/farmers or under field condition thereby obviating the need for any laboratory facilities. Further object of the present invention is to propose a process of testing urea in milk and an indicator solution thereof, which is simple and can be easily visualized by appearance of a coloured reaction of urea with the indicator of the present invention. Still further object of the present invention is to propose a process of testing urea in milk and an indicator solution thereof, which is economical. Further object and advantages of this invention will be more apparent from the ensuing description. STATEMENT OF THE INVENTION According to this invention there is provided a process of testing urea in milk comprising steps of putting a drop of indicator solution on a filter paper, putting an equal amount of milk to be tested on the indicator solution drop wherein the indicator solution comprises mixture of solution A and B in which solution A is prepared by dissolving P-Dimethylamino Benzaldehyde in a solvent followed by addition of Hydrochloric acid in the solution thus obtained, Solution B is prepared by dissolving Methylred and Bromo cresol green in the solvent. Further according to this invention there is provided an indicator solution comprising of P-dimethylamino Benzaldehyde in solvent, hydrochloric acid, methyl red and Bromo Cresol green in the solvent. DETAIL DESCRIPTION OF THE INVENTION In carrying out test, of the present invention, a drop of indicator solution of the present invention, which is pink in colour, is put on a filter paper and allowed to be absorbed. An equal amount of test milk sample is put on the indicator solution drop. A yellow colour gets developed immediately around the milk drop. This yellow colour forms a ring around the milk drop slowly. This indicates a positive case of urea in milk. In negative cases, the area around the milk drop remains pink or white coloured immediately after putting a milk drop. In case of suspected greater dilution of synthetic milk-for example 10.0 ml of test milk sample is taken and it is boiled for sometime so that it gets concentrated to about its half i.e. 5 ml. This concentrated test milk, sample is then put on a drop of indicator solution on a filter paper. The appearance of yellow ring around the milk drop shows presence of urea.The indicator solution of the instant invention comprises two solutions i.e. solution A and B. Solution A is prepared by dissolving 1.6 to 1.8% of P-Dimethylamino Benzaldehyde in a solvent, which is dehydrated alcohol for example Ethanol. The solution thus prepared is added with 9-11% of Hydrochloric acid to obtain solution A. The solution B is prepared by dissolving 0.6-0.7% of methyl red and 0.3-0.4% of Bromo cresol green in a solvent of dehydrated alcohol for example Ethanol. The indicator solution of the instant invention is obtained by dissolving 0.9 to 1.1% of solution B into whole of the solution A. The whole process is carried out at room temperature. It is to be noted that the present invention is susceptible to modifications, adaptations and changes by those skilled in the art. Such variant embodiments employing the concepts and features of this invention are intended to be within the scope of the present invention, which is further set forth under the following claims:- WE CLAIM: 1. A process of testing urea in milk comprising of: i) a filter paper, milk and indicator solution, ii) putting a drop of indicator solution on a filter paper, iii) an equal amount of milk to be tested is put on the indicator solution drop, characterized by the said indicator solution comprising a mixture of solution A and B, iv) the said solution A is obtained by dissolving 1.6-1.8% P- Dimethylamino Benzaldehyde in dehydrated alcohol such as ethanol followed by adding 9-11% of Hydrochloric acid, v) the said solution B is obtained by dissolving 0.6-0.7% of Methyl Red and 0.3 -0.4% Bromo cresol green in a solvent as herein described, vi) solution B is added in solution A in an amount of 0.9 to 1.1% to obtain the indicator. |
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822-DEL-2004-Abstract-(11-11-2011).pdf
822-DEL-2004-Abstract-(18-06-2009).pdf
822-DEL-2004-Assignment-10-06-2008.pdf
822-DEL-2004-Claims-(11-11-2011).pdf
822-DEL-2004-Claims-(18-06-2009).pdf
822-DEL-2004-Correspondence Others-(11-11-2011).pdf
822-DEL-2004-Correspondence-Others-(18-06-2009).pdf
822-DEL-2004-Correspondence-Others-10-06-2008.pdf
822-del-2004-correspondence-others.pdf
822-del-2004-correspondence-po.pdf
822-DEL-2004-Corresponence-Others-(10-07-2009).pdf
822-DEL-2004-Description (Complete)-(18-06-2009).pdf
822-del-2004-description (complete).pdf
822-del-2004-description (provisional).pdf
822-DEL-2004-Form-1-(10-07-2009).pdf
822-DEL-2004-Form-1-(11-11-2011).pdf
822-DEL-2004-Form-1-(18-06-2009).pdf
822-del-2004-form-2 (complete).pdf
822-DEL-2004-Form-2-(18-06-2009).pdf
822-del-2004-Form-2-(19-12-2011).pdf
822-DEL-2004-Form-5-(18-06-2009).pdf
822-DEL-2004-GPA-(10-07-2009).pdf
822-DEL-2004-GPA-(11-11-2011).pdf
822-DEL-2004-Petition-137-(11-11-2011).pdf
| Patent Number | 250500 | |||||||||
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| Indian Patent Application Number | 822/DEL/2004 | |||||||||
| PG Journal Number | 02/2012 | |||||||||
| Publication Date | 13-Jan-2012 | |||||||||
| Grant Date | 06-Jan-2012 | |||||||||
| Date of Filing | 05-May-2004 | |||||||||
| Name of Patentee | NATIONAL RESEARCH DEVELOPMENT CORPORATION (A GOVERNMENT OF INDIA ENTERPRISE) | |||||||||
| Applicant Address | ANUSANDHAN VIKAS,20-22, ZAMROODPUR COMMUNITY CENTRE, KAILASH COLONY EXTENSION NEW DELHI-110048 | |||||||||
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| PCT International Classification Number | C07 | |||||||||
| PCT International Application Number | N/A | |||||||||
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