| Abstract |
A composition useful in developing an artificial bone marrow-like environment (ABME) for regulating formulating of blood cells, comprising: a) a culture of mesenchymal cells; b) a growth medium selected from Iscove’s modified Dulbecco’s Medium (IMDM), Dulbecco’s modified eagle medium (DMEM), Alpha-minimum essential medium (MEM), RPMI-1640 supplemented with Fetal Bovine Serum (FBS) and optionally supplemented with mthyl cellulse, erythropoietin, hematopoietic growth and differentiation factors, Interleukins; c) a hematopoietic modulator or plurality of modulators capable of activating intracellular signalling; wherein the hematopoietic modulator(s) such as hereindescribed selected from any of: a biological agent, a chemical agent and an immunological agent; and d) a support for the cells comprising constituents of extra cellular matrix or their mimetics capable of forming a matrix in two or more dimensions. |