Title of Invention

A METHOD FOR PREPARING A DIETARY SUPPLEMENT COMPOSITION FOR REDUCING BODY WEIGHT

Abstract The invention discloses a method for preparing a dietary supplement composition for reducing body weight wherein said method comprises mixing the calcium salt of hydroxycitric acid, and at least one of the potassium salt of hydroxycitric acid or the sodium salt of hydroxycitric acid, wherein the said composition comprises more than 40% by weight and less than 90% by weight of total hydroxycitric acid, wherein the said composition comprises between: a) 5% by weight to 13% by weight calcium; and b) 9% by weight to 20% by weight potassium; or c) 5% by weight to 12% by weight sodium; or a mixture thereof, each calculated as a percentage of the total weight of said hydroxycitric acid composition.
Full Text

Technical Field
The present invention relates to method of manufacturing hydroxycitric acid compositions, dietary
supplements and food products containing such compositions, and to the use of such compositions
and products to reduce body weight in mammals. This application is divided out of Indian Patent
Application No. 268KOL2003 which is a divisional of 66CAL2001 which is a divisional of
1223CAL98.
Background of the Invention
Hydroxycitric Acid has been known for many years to be beneficial for the control and reduction of
mammalian body weight. In particular, a specific stereoisomer of hydroxycitric acid, the (-)
hydroxycitric acid isomer and derivatives thereof, is known to inhibit fatty acid and cholesterol
synthesis and to function as a natural anorectic agent in mammals.
The stereoisomers of hydroxycitric acid are related structurally to citric acid wherein a hydroxy
group is substituted for one of the four methylene hydrogens. Thus, there are four possible
stereoisomers of hydroxycitric acid. Of these four stereoisomers, the (-) hydroxycitric acid isomer
has been found to substantially inhibit fatty acid synthesis in biological systems in profused organs
and intact mammals, and particularly in non-ruminant mammals.
It has also been known that the particular stereoisomer of interest, in both free acid and lactone
forms, is found in the rind of the fruits of Garcinia species, for example, Garcinia cambogia,
Garcinia arroviridis and Garcinia indica, which are native to the Indian subcontinent. The
hydroxycitric acid component can be obtained by isolation from the fruit of Garcinia species using
known procedures, for example Lewis, Y.S. "Methods in Enzymology" (J.M. Lowenstein, Ed., Vol.
13, pg. 613) (Academic Press, N.Y. 1969), and U.S. Patent No. 5,536,516.


As an inhibitor of the synthesis of fatty acids and cholesterol, hydroxycitric acid has been shown to
significantly reduce the body weight and lower lipid accumulation in rats. See, e.g. Sergio, W.
Medical Hypothesis 27:39 (1988), Sullivan, A.C., et al., Lipids 9:121 (1973), and Sullivan, A.C., et
al., Lipids 9:129 (1973).
However, in order to formulate the compositions containing hydroxycitric acid into dietary
supplements and food products, a number of desirable properties are sought. First, as most such
supplements and food products are administered orally, the composition should have negligible
odor and taste. Second, in order to increase the bioavailability of the hydroxycitric acid, the
composition should be soluble in water. Solubility in water, as well as clarity in solution, are also
important properties in many food and beverage applications. In addition, it is highly desirable that
the composition be non-hygroscopic, in order to facilitate storage and formulation into dietary
supplements and food products. Another desirable property is the hydroxycitric acid potency of the
composition, where high potency levels are desirable.
DISCLOSURE OF THE INVENTION
The present invention provides hydroxycitric acid compositions, food products made therefrom and
methods for their use in reducing body weight.
Accordingly, in one aspect, the present invention provides a method for manufacturing a
hydroxycitric acid composition for reducing body weight wherein said method comprises
formulating a composition comprising a mixture of the calcium salt of hydroxycitric acid and at
least one of the potassium salt of hydroxycitric acid and the sodium salt of hydroxycitric acid,
wherein said composition comprises
a) approximately 14 to 26% by weight of calcium, and
b) approximately 24 to 40% by weight of potassium or approximately 14 to 24% by weight of
sodium, or a mixture thereof, calculated as a percentage of the total hydroxycitric acid content of
said composition.
In another aspect of the invention, a method of manufacturing a composition for reducing body
weight is provided which comprises at least approximately 40% by weight of total hydroxycitric

acids which further comprises approximately 5 to 13 % by weight of calcium, and approximately 9
to 20% by weight of potassium or approximately 5 to12% by weight of sodium, or a mixture
thereof, calculated as a percentage of the total weight of said composition.
Additional aspects of the invention include method of manufacturing dietary supplements and food
products for use in reducing body weight which include the present compositions, and methods for
reducing body weight by administering such compositions, dietary supplements and food products
to mammals.
Detailed Description of the Invention
The present invention provides method for manufacturing hydroxycitric acid compositions, dietary
supplements and food products made therefrom and methods for their use in reducing body weight.
In one aspect, the invention provides method for manufacturing a hydroxycitric acid composition
for reducing body weight wherein the composition comprises approximately 14 to 26% by weight
of calcium, and approximately 24 to 40% by weight of potassium or approximately 14 to 24% "By
weight of sodium, or a mixture thereof, calculated as a percentage of the total hydroxycitric acid
content of said composition.
Hydroxycitric acid has been known for years to be useful for inhibiting fatty acid synthesis. Citrate
is formed in the mitochondria by the citrate synthase reaction. It is then metabolized via the citric
acid cycle. Under certain metabolic conditions, some citrate is diverted to the cell cytosol where it
is used for fatty acid synthesis, that is, for energy storage. The inhibition of fatty acid synthesis in
biological systems by the use of hydroxycitric acid is believed to arise from the inhibition of the
citrate cleavage enzyme citrate-lyase by such compounds. The cleavage of citrate is catalyzed by
citrate-lyase and citrate is the major source of the acetyl group of acetyl coenzyme A which is
utilized in the conversion of carbohydrates and various amino acids to fats by non-ruminant
mammals.
Typically, hydroxycitric acid is utilized in the form of its pharmaceutically acceptable, non-toxic
basic salts. Such salts include, for example, the alkali metals, e.g. sodium and potassium, the
alkaline earth metals, e.g. calcium and magnesium, and complex salts, such as ammonium or
substituted ammonium salts.


In preparing various salts of hydroxycitric acid, it has been found that the pure potassium salt was
highly soluble in water, but possessed high hygroscopicity, an undesirable property. It was also
determined that the pure calcium salt was moderately soluble in water, and possessed minimal
hygroscopicity, a desirable property.
Surprisingly, it has been determined that a mixture of calcium salt with potassium or sodium salts
produces a composition which is highly soluble (up to25 % weight to volume in water), but with
minimal hygroscopicity.
Furthermore, the composition according to the present invention displays minimal palatability
concerns, as the taste of free hydroxycitric acid is almost entirely eliminated, as well as exceptional
product application properties, including negligible odor, taste and color. While normally brown,
the composition was found to be clear in solution.
In addition, the composition was found to be largely free of the lactone form of hydroxycitric acid,
and that in solution it did not equilibrate between the free and lactone forms. Further desirable
properties included a balanced pH, and a low( acceptable replacement for potassium in the present compositions, its inclusion is considered
undesirable for considerations of minimizing dietary intake.
Thus the present compositions demonstrate a surprising, synergistic relationship between the
calcium content and the potassium (or sodium) content. It has been determined that for a
composition where the total hydroxycitric acid content exceeds 40% by weight, a total of salts of
hydroxycitric acid which desirably comprise approximately 5 to 13 % by weight of calcium,
preferably approximately 7 to 13 %, and approximately 9 to 20% by weight of potassium,
preferably approximately 14 to 18%, or approximately 5 to 10% by weight of sodium, or mixtures
of potassium and sodium. As stated earlier, although sodium is acceptable, it is considered
desirable to minimize the content of sodium, desirably to less than 1 % by weight.
It is further considered desirable to provide a method of manufacturing a composition in which the
total hydroxycitric acid content is at least approximately 40% by weight, preferably at least


approximately 50 % , and desirably approximately 55-65 %. Thus, in another aspect of the
invention, a method for manufacturing a composition for reducing body weight is provided which
comprises at least approximately 50% by weight of total hydroxycitric acids which further
comprise approximately 5 to 13% by weight of calcium, and approximately 9 to 20% by weight of
potassium or approximately 7 to 12% by weight of sodium, or a mixture thereof, calculated as a
percentage of the total weight of said composition. Of the total amount, it is also considered
desirable that the amount of hydroxycitric acid in the form of the lactone not exceed approximately
2 % by weight.
It is considered desirable to enrich the purity of free hydroxycitric acid from the Garcinia rind and
prepare a calcium salt of the hydroxycitric acid. Generally, commercially available Garcinia rind
comprises 25 to 30% moisture and 2 to 5 % of sodium chloride. Garcinia rind contains 10 to 12%
of free hydroxycitric acid, 12 to 15% of the lactone form of hydroxycitric acid and 2 to 3 % citric
acid on dry weight of the rind.
A further aspect of the preparation of the salt of hydroxycitric acid is to mask the sour taste of
hydroxycitric acid, minimize the percentage of hydroxycitric acid lactone and prepare a sodium
free salt of hydroxycitric acid. The process generally comprises washing the Garcinia rind,
extracting hydroxycitric acid from the Garcinia rind, preparing an insoluble calcium salt of the
hydroxycitric acid, dissociating the insoluble calcium salt and thereafter preparing calcium and
potassium salts of hydroxycitric acid. The washing of the Garcinia rind is optional, as the sodium
chloride can be reduced at other stages in the processing.
The salt free water extract can be obtained from salted Garcinia rind by washing the Garcinia rind
followed by hot extraction. The dilute water extract is filtered through a filtrate after adding clay to
the extract and settled. The filtered dilute extract is then concentrated to45 % total solids under
reduced pressure at elevated temperature. This concentrate contains 10 to 12% of free
hydroxycitric acid by weight, 11 to 13% of hydroxycitric acid lactone by weight and 2 to 3 % of
citric acid by weight.
The content of free hydroxycitric acid, hydroxycitric acid lactone, citric acid and non acid solutes
can be determined by known techniques.

The process of enriching free hydroxycitric acid from the rind is accomplished by preparing a water
extract of the rind, converting the extract into a insoluble calcium citrate and removing non acidic
impurities in the extract, such as pectin, sugar and color which will solubilize, by washing the
calcium hydroxycitrate. Thereafter, the calcium hydroxycitrate is dissociated with dilute phosphoric
acid to form hydroxycitric acid and calcium phosphate. The calcium phosphate is then filtered out
and the enriched hydroxycitric acid solution is converted to highly soluble calcium salt by first
adjusting the pH of the hydroxycitric acid solution to 3.5 to 5 with calcium hydroxide suspension
and second by the addition of potassium hydroxide solution to adjust the final pH to 8.0 to 9.0. This
calcium salt solution is then filtered and concentrated under reduced pressure to approximate 50%
total solids. The concentrate is then treated with 75 % alcohol/acetone to crystalize white
crystalline highly soluble calcium salt.
There are numerous protocols available for preparing hydroxycitric acid extracts from Garcinia
fruits. The rind of the Garcinia fruit which is commercially available typically consists of
approximately 20% hydroxycitric acid, approximately 25 % moisture and approximately 2.5%
sodium chloride. It is considered desirable to eliminate as much sodium chloride from the rind as
possible and since sodium chloride is freely soluble in water, this is easily accomplished. For
example, one kilogram of raw Garcinia rind material is washed with approximately 2 liters of water
in a stainless steel vessel. Thereafter, salt free Garcinia rind is extracted with 55 to 60°C water on a
continuing basis with approximately 15 liters of water. The spent rind is then tested for
hydroxycitric acid content and typically discarded. At this stage, recovery of total hydroxycitric
acid is on the order of 90±2% .
Next, approximately 2.5 kilograms of Fuller's Earth (a kaolin containing an aluminum magnesium
silicate) is added to the solution obtained previously. The mixture is stirred for one hour with
continuous agitation at approximately 100 revolutions per minute then allowed to settle for two
hours. Thereafter, the material is filtered through a bed of a filter aid in a centrifuge. The filtrate is
concentrated to 800 grams of approximately 45% total solids containing approximately 22% total
hydroxycitric acid content. The yield at this step is approximately 97% of the extracted
hydroxycitric acid.

Next, the concentrated extract is filtered in a centrifuge to remove solids. To the filtered extract is
added calcium hydroxide (100 grams in 500ml of water). The mixture is stirred for approximately
four hours maintaining the pH of the solution at approximately 8.5. Thereafter, the mixture is
filtered through the centrifuge and the supernatant is discarded. The moist pellet is washed
continuously with 5 liters of water until the water is colorless and no solids are extracted from the
filtrate. The yield of hydroxycitric acid at this phase is approximately 96.5 %. In the next step, the
wet pellet of calcium hydroxycitrate obtained previously is treated with 500ml of 2N phosphoric
acid solution to convert the calcium hydroxycitrate to hydroxycitric acid and calcium phosphate.
Calcium phosphate precipitate is removed by centrifugation and washed with 2 liters of water. The
filtrate contains approximately 165 grams of hydroxycitric acid with total solids of approximately
6.8 % and the hydroxycitric acid yield is approximately 91.6%.
Finally, the hydroxycitric acid solution obtained previously is treated with calcium hydroxide (61.7
grams in 600 ml of water to adjust to pH 4.5). This solution is treated with neutral charcoal (60
grams at 75°C for two hours under agitation, cooled and filtered). To the filtrate is added potassium
hydroxide (52 grams in 50 ml of water), which adjusts the pH to between 8.0 and 8.5. This salt
solution is concentrated to 555 grams under reduced pressure to provide 50% total solids. The
concentrate is treated with 75 % acetone to obtain pure crystalline highly soluble calcium salt of
hydroxycitric acid.
The amount of (-) hydroxycitric acid can be estimated by high pressure liquid chromatography
(HPLC), generally as follows.
Estimation of (-) HCA by HPLC:
HPLC System: SHIMADZU or equivalent
LC 10AT Pump or equivalent
SPD 10 Detector or equivalent
CR 10A Software or equivalent
Column: ALLTIMA C12(5µ) (4.6x250 mm)
Wave Length: 210nm
Flow rate : 1 mL/min
Volume of Injection:20µL


Temperature:25°±2°C.
Mobile Phase: 0.05M sodium sulphate solution in water (pH adjusted to approximately 2.3 with
cone. H2SO4).
Standards: 1) Ethylenediamine Salt of (-) HCA.
2) (-) HCA Lactone.
Standards Preparation: Weigh accurately about 50mg of each standard into two
different 25mL volumetric flasks. Dissolve in water and make up to volume
with water. Filter through 0.22µ. membrane filter and inject the standard
solutions separately.
Sample preparation: Weigh accurately about 50mg of sample in a 25mL
volumetric flask. Dissolve it in water and make up the volume with water.
Filter through 0.22µ membrane filter and inject the solution.
Retention Times: for free (-) HCA - 5 min.
(-) HCA Lactone - 4.1 min.
Calculations: % of Free (-) HCA = Sample Area x Standard Cone. x Purity of Standard
Standard Area x Sample Cone.
% of (-) HCA Lactone = Sample Lactone Area x Lactone Standard Conc, x Purity of Lactone
Lactone Standard Area x Sample Conc.
REAGENTS
Unless otherwise stated all chemicals used are reagent grade.
All glass washed with double distilled water.
Dilute ammonia solution,25% w/v.
Ammonium chloride.
EDTA
Mordant Black T mixture

Sodium sulphate, analytical grade, or equivalent
Sulphuric acid, analytical grade or equivalent
WATER SOLUBLE EXTRACTIVES: As per USP XX.
pH : As per USP (1% Solution)
MOISTURE CONTENT : As per USP (K.F. Titrimeter)
CALCIUM : Weigh accurately about 10mg of sample into a 100mL conical flask,
dissolve it in50mL of water. Add 2mL of ammonia - ammonium
chloride (pH 9.2) buffer. Then titrate with 0.01M EDTA solution.
Using Mordant Black-T Mixture as indicator. End point is blue.
% of Calcium = Titre value x Molarity of EDTA x 0.4 x 100
0.01 x Weight of the sample in mg.
ESTIMATION OF SODIUM AND POTASSIUM BY FLAME PHOTOMETER:
Place 100mg of sample in a Silica crucible, and reduce to ash in a muffle furnace at 400°C.
Transfer ash into a 50mL volumetric flask, add 1 drop cone. HCl and water to dissolve and make
up to volume with water.
Calibrate the flame photometer with 100, 50 and 10ppm standard sodium and potassium solutions.
Now place the sample solution in the flame photometer. Note the ppm reading corresponding to
Sodium and Potassium.
% of corresponding Ion = ppm reading corresponding Ion x 50 x 100
1000 x Weight of sample in mg.
By inhibiting the synthesis of fatty acids, hydroxycitric acid is useful for the reduction of body
weight in mammals. These useful compositions can be provided in the form of conventional
pharmaceutical preparations or dietary supplements, for example, they can be mixed with
conventional organic or inorganic inert pharmaceutical carriers or dietary supplements suitable for
oral or parenteral administration, such as, for example, water, gelatin, lactose, starch, magnesium
stearate, talc, vegetable oil, gums or the like. They can be administered in conventional forms, e.g.,


solid forms, for example powders, tablets, capsules, suppositories or the like; or in liquid forms, for
example, suspensions or emulsions. In addition, such compositions can be formulated as a part of a
processed food product for example in a form of a bar, baked good, beverage and the like.
Moreover, the pharmaceutical compositions and dietary supplements can be subject to conventional
pharmaceutical or dietary supplements expedients, such as sterilization, and can contain
conventional pharmaceutical or dietary supplements excipients, such as preservatives, stabilizing
agents, emulsifying agents, salts for the adjustment of osmotic pressure or buffers, and the like. The
compositions can also contain other therapeutically active materials.
A suitable dosage unit will typically contain from about 15 to about 3000mg of hydroxycitric acid,
administered up to three times per day. Suitable parenteral dosage regimens in mammals can
comprise from about 1 mg per kilogram of body weight to about 50mg per kilogram of body weight
per day. However, for any particular subject, the specific dosage regimen should be adjusted
according to individual need and the professional judgment of the person administering or
supervising the administration of the aforesaid compounds.
Additional aspects of the invention include method for manufacturing food products and the like
for use in reducing body weight which include the present compositions, and methods for reducing
body weight by administering such compositions and dietary supplements and food products. When
provided for oral administration as a processed food product, such as a beverage or a snack bar, the
hydroxycitric acid content will desirably comprise approximately 0.001 to 25%, preferably 0.05 to
5% by weight of the total weight of the food product.
Preparation of processed food products to include hydroxycitric acid compositions of the present
invention involves, for example, diluting a concentrate of the composition containing at least
approximately 40% hydroxycitric acid in water, adding supplements, blending, heating and/or
periodic agitation.
For both snack bars and beverages, it is desirable to pre-pasteurize the concentrate in a highly
diluted ratio with purified water. For example, approximately 1 mL of the concentrate can be added
to 12 fluid ounces of water. These figures will however vary depending upon the types of products


desired, ranging from 1 to 25 % for a beverage and 1 to 40% for a snack bar. After the blending
step, the temperature of the vessel which the preprocessing step takes place is increased, frequently
using steam as in the case of beverage manufacture or low heat as in the case of snack bars and
baked goods. Before heat is applied, materials such as nutrients, antioxidants, vitamins and
minerals can be added. In the production of the beverage, after the supplementation of the desired
additives and achievement of homogeneosity, the liquid is pumped by a stainless steel pipeline into
a bottling facility. High temperature steam is applied from the outside to the pipes which in turn
maintains the temperature of the liquid during its transit. The pipes are placed so as to facilitate the
bottling of the liquid beverage in an efficient manner.
In the preparation of, for example, snack bars, the preprocessing follows approximately the same
protocol as for beverages. The environment for the development of this product is typically an
industrial kitchen with the use of large cooking pots. The diluted hydroxycitric acid composition is
blended with water, covered and heated, bringing it to a boil for a certain period of time. This
boiling also provides agitation to insure thorough mixing. Thereafter, snack bars, baked goods or
other processed food products are produced in accordance with the techniques well known in the
art.
Although the foregoing invention has been described in some detail by way of illustration and
example for purposes of clarity and understanding, it will be apparent to those of ordinary skill in
the art in light of the disclosure that certain changes and modifications may be made thereto
without departing from the spirit or scope of the appended claims.


WE CLAIM:
1.A method for preparing a dietary supplement composition for reducing body weight
wherein said method comprises mixing the calcium salt of hydroxycitric acid, and at
least one of the potassium salt of hydroxycitric acid or the sodium salt of
hydroxycitric acid, wherein the said composition comprises more than 40% by weight
and less than 90% by weight of total hydroxycitric acid, wherein the said composition
comprises between:
a) 5% by weight to 13% by weight calcium; and
b) 9% by weight to 20% by weight potassium; or
c) 5% by weight to 12% by weight sodium;
or a mixture thereof, each calculated as a percentage of the total
weight of said hydroxycitric acid composition.
2.A method as claimed in claim 1, wherein the lactone forms of hydroxycitric acid
comprise less than 2% by weight of the total hydroxycitric acid content of the
composition.
3.A method as claimed in claim 1, wherein the calcium comprises 9 to 13% by
weight.
4.A method as claimed in claim 1, wherein the potassium comprises 14 to 18% by
weight.
5.A method for preparing a dietary supplement composition for reducing body weight
wherein said method comprises mixing the calcium salt of hydroxycitric acid, and at
least one of the potassium salt of hydroxycitric acid or the sodium salt of
hydroxycitric acid, wherein the said composition comprises more than 50% by weight
and less than 90% by weight of totally hydroxycitric acid, wherein the said
composition comprises between:
a) 5% by weight to 13% by weight calcium; and
b) 9% by weight to 20% by weight potassium; or
c) 5% by weight to 12% by weight sodium;

or a mixture thereof, each calculated as a percentage of the total
weight of said hydroxycitric acid composition.
6.A method as claimed in claim 1, wherein the hydroxycitric acid composition
comprises 55-65% by weight.
7.A method as claimed in claim 1, wherein the lactone forms of hydroxycitric acid
comprises less than 4% by weight of the total weight of the composition.



The invention discloses a method for preparing a dietary supplement composition for
reducing body weight wherein said method comprises mixing the calcium salt of
hydroxycitric acid, and at least one of the potassium salt of hydroxycitric acid or the
sodium salt of hydroxycitric acid, wherein the said composition comprises more than
40% by weight and less than 90% by weight of total hydroxycitric acid, wherein the
said composition comprises between:
a) 5% by weight to 13% by weight calcium; and
b) 9% by weight to 20% by weight potassium; or
c) 5% by weight to 12% by weight sodium;
or a mixture thereof, each calculated as a percentage of the total weight
of said hydroxycitric acid composition.

Documents:

00759-kol-2005-abstract.pdf

00759-kol-2005-claims.pdf

00759-kol-2005-description complete.pdf

00759-kol-2005-form 1.pdf

00759-kol-2005-form 2.pdf

00759-kol-2005-form 3.pdf

00759-kol-2005-form 5.pdf

759-KOL-2005-ABSTRACT-1.1.pdf

759-kol-2005-assignment.pdf

759-KOL-2005-CANCELLED PAGES.pdf

759-KOL-2005-CLAIMS-1.1.pdf

759-kol-2005-correspondence.pdf

759-KOL-2005-DESCRIPTION (COMPLETED)-1.1.pdf

759-kol-2005-examination report.pdf

759-KOL-2005-FORM 1-1.1.pdf

759-kol-2005-form 18.pdf

759-KOL-2005-FORM 2-1.1.pdf

759-KOL-2005-FORM 3-1.1.pdf

759-kol-2005-form 3.pdf

759-KOL-2005-FORM-27.pdf

759-kol-2005-gpa.pdf

759-kol-2005-granted-abstract.pdf

759-kol-2005-granted-claims.pdf

759-kol-2005-granted-description (complete).pdf

759-kol-2005-granted-form 1.pdf

759-kol-2005-granted-form 2.pdf

759-kol-2005-granted-specification.pdf

759-kol-2005-others.pdf

759-KOL-2005-PETITION UNDER RULE 137.pdf

759-kol-2005-reply to examination report-1.1.pdf

759-KOL-2005-REPLY TO EXAMINATION REPORT.pdf

759-kol-2005-translated copy of priority document.pdf


Patent Number 246155
Indian Patent Application Number 759/KOL/2005
PG Journal Number 08/2011
Publication Date 25-Feb-2011
Grant Date 17-Feb-2011
Date of Filing 19-Aug-2005
Name of Patentee INTERHEALTH NUTRACEUTICALS INCORPORATED
Applicant Address 1320 GALAXY WAY, CONCORD, CALIFORNIA 94520, UNITED STATES OF AMERICA
Inventors:
# Inventor's Name Inventor's Address
1 GANGA RAJU G BRINDAVAN COLONY, VIJAYAWADA 520-010, INDIA
PCT International Classification Number C07C 059/265
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 08/892414 1997-07-14 U.S.A.