Title of Invention

"METHOD OF OBTAINING URETHANE-PROTECTED N-CARBOXYANHYDRIDES OF ALPHA AMINO ACIDS"

Abstract The invention relates to a method of preparing urethane-protected N-carboxyanhydrides of alpha amino acids. The inventive method enables the synthesis of urethane-protected N-carboxyanhydrides of alpha amino acids in the presence of a catalytic quantity of triethylene diamine without the addition of a tertiary amine-type base.
Full Text METHOD OF OBTAINING URBTHANE-PROTECTED N-CARBOXYANHYDRIDES OF
ALPHA AMINO ACIDS
The method relates to a novel method for preparing uretharie-protected N-carboxyanhydrides of alpha amino acids. With the novel method, urethane-protected N-carboxyanhydrides of alpha amino acids may be synthetized from N-carboxyanhydrides of alpha amino acids in the presence of a catalytic amount of triethylene diamine, without adding significant: amounts of a base of the tertiary amine type.
N-carboxyanhydrides of alpha amino acids (designated under the acronym of NCAs), optionally protected, are acylation agents often used for forming high molecular weight polyalpha-amino acids and for producing dipeptides. NCAs are very reactive compounds, which do not form, notably by rearrangement, any undesired secondary products and their unique reaction byproduct is carbon dioxide. As soon as NCA is reacted with a free amine function of an amino acid, carbon dioxide is immediately released and a dipeptide is formed, which itself also contains a free amine function. This amine will react with NCA and form a tripeptide and so forth. NCAs may thereby be used in forming poly(alpha amino acids) but they cannot easily be used in the sequential synthesis of polypeptides, as multiple condensation secondary reactions such as oligomerisation, are difficult to control.
Alpha amino acid N-carboxyanhydrides substituted with urethane groups have been described in the literature, they are used in peptide syntheses. The urethane substituent provides a high degree of protection and allows polymerisation reactions to be minimized during the coupling reaction. Urethane-protected NCAs, abbreviated as UNCAs hereafter, have all the advantages of non-substituted NCAs without the drawbacks of the latter.
UNCAs allow controlled synthesis of polypeptides without requiring any pre-activation of the carboxyl groups and without requiring any addition of additives such as N-hydroxybenzot.riazole. Thus, purification of the peptides
produced in solution is facilitated, since the only byproduct of the peptide synthesis reaction is carbon dioxide.
UNCAs are also very useful as raw materials in the synthesis of hormones or anti-AIDS drugs.
UNCAs which are in a crystalline form under room temperatures and pressure conditions, are stable under standard laboratory handling and storage conditions and under peptide synthesis conditions.
The main two routes for synthesis of UNCAs from NCAs are the following:
1) UNCAs may be synthetized by condensation of an alkyl or aralkyl chloroformate, such as Fmoc-Cl (9-f luorenylmethyloxycarbonyl chloroformate) or benzyl chloroformate, with an NCA in presence of at least a stoichiometric amount of a tertiary amine. This tertiary amine which conventionally is N-methylmorpho1ine , the released hydrochloric acid to scavenged. NCA is thereby put into solution in an inner solvent, such as THF, and cooled. 1.1 to 1.3 equivalents of alkyl or aralkyl chloroformate is added only once and then at least 1.5 equivalents of tertiary amine, for example N-methylmorpholine, is slowly added. The resulting suspension is left at rest for 1 to 2 hours, at a temperature between -25 and -5°C. Next, hydrochloric acid dissolved in dioxane is slowly added until pH values of about 4-5 are obtained. The thereby formed hydrochloride of the tertiary arnine is filtered off and UNCA is concentrated and crystallized.
All the steps of the method are performed under an inert atmosphere (N2) and all the solvents are dried on a 4 A molecular sieve before being used (William D. Fuller et al. , Urethane-protected-alpha-amino acid N-carboxyanhydrides and peptide synthesis, Biopolymers, 1996, 40, 183-205).
This synthesis route is not very suitable for preparing certain protected alpha amino acid N-carboxyanhydrides, notably those protected by a t-butoxylcarbonyl radical, t-butyl chloroformate being very unstable above -20°C or in the presence of tertiary amines.
2) UNCAs may also be synthetized by condensation of a dialkyl dicarbonate with an NCA. This reaction releases an alcohol molecule and a carbon dioxide molecule. This synthesis must absolutely be carried out in the presence of a large amount, at least a 50% molar amount relatively to the engaged NCA molar amount, of a tertiary amine such as N-methylmorpholine associated with a catalytic amount of DMAP (4-dimethylamino-pyridine) or a pyridine (William D. Fuller et al., Urethane-protected-alpha-amino acid N-carboxyanhydrides and peptide synthesis, Biopolymers, 1996, 40, 183-205) . This synthesis route is particularly suited for the synthesis of alpha-amino acid N-carboxyanhydrides protected by a t-butoxylcarbonyl radical by using di-tertiobutyl dicarbonate.
Application WO89/08643 describes N-carboxy-anhydrides of alpha-amino acids and N-thiocarboxy-anhydrides of alpha-amino acids with urethane protection, of formula,
(Formula Removed)
wherein R and R' represent a hydrogen atom, an alkyl, cycloalkyl, cycloalkyl radical substituted with a substituted alkyl, aryl or substituted aryl radical, and at least one R or R1 group does not represent a hydrogen atom; R" represents an alkyl, aryl, substituted alkyl or substituted aryl; Z represents an oxygen or sulphur atom and n is 0, 1 or 2.
These compounds are prepared by reaction of NCA with a haloformate in an inert solvent, such as toluene, under anhydrous conditions, in the presence of an tertiary amine type base added in excess.
The existing methods for synthetizing Incas are not satisfactory. Actually, the best method described above, which uses a base of the tertiary amine type, in an amount at least equal to a 50% molar amount relatively to the amount of
engaged NCA, provides yields of only about 60%, provided that the solvents are dried on a 4 A molecular sieve and that one operates between -20 and -15°C.
Surprisingly, it was discovered that the use of triethylene diamine (TEDA) in a very low catalytic amount, less than 5% molar relatively to the molar amount of engaged NCA, without adding any tertiary amine type base, leads to excellent results.
Within the scope of the present invention, the acronym NCA(s) designates alpha-amino acid N- carboxy- anhydride (s) and UNCA(s) designates urethane-protected alpha-amino acid N-carboxyanhydride (s) .
In the sense of the present invention, a catalytic amount means an amount significantly less and more specifically, less than 50% than that required by stoichiometry .
The present invention relates to a method for obtaining urethane-protected alpha-amino acid N-carboxy-anhydrides (IJNCAs) , of formula I
(Formula Removed)
wherein R1 and R2 , either identical or different, together or independently of each other, represent a hydrogen atom or a side chain of a natural or synthetic alpha-amino acid optionally bearing functional groups, if necessary protected; R5 represents a linear or branched, saturated or insaturated, CJ.-CIQ alkyl radical or an aralkyl or alkaryl radical with 7 to 14 carbon atoms, characterized in that an alpha-amino acid N-carboxyanhydride (NCA) of formula
(Formula Removed)
wherein R1 and R2 have the same meaning as for formula I, is reacted with at least one equivalent, relatively to the engaged molar amount of NCA with formula II, of the
dicarbonate of formula III
wherein R3 has the same meaning as for formula I, in the presence of a catalytic amount of 1, 4-diazabicyclo[2.2.2]octane, also designated as triethylene diamine (TEDA), relatively to the engaged molar amount of NCA of formula II, in an inert organic solvent with a melting point less than about -20°C.
A natural or synthetic alpha-amino acid is an amino acid bearing on the first carbon of the chain, an amine function and a carboxylic acid function. The remainder of the alpha-amino acid is called the side chain of the alpha-amino acid.
R1 and R2 are if necessary protected with protective
groups currently used in the field of amino acids and peptides
(Bodansky, Principle of peptide synthesis, Springer-Verlag,
1984; Alpha amino-acid N-carboxyanhydrides and related
heterocycles, Hans R. Kricherdorf) .
R1 and R2, either identical or different, advantageously represent a hydrogen atom, a linear or branched Ci-C8 alkyl radical optionally comprising one or more customary substituents in the field of amino acids and peptides. The substituents are notably selected from the group formed by OH, SH, NH2, NHC(NH)NH2, CONH2, O- (C6-C10-aryl) , S- (C1-C6 alkyl), COO-(Cx-Cg alkyl), COO-(C5-C8 aralkyl), notably the benzyl ester radical .
A group R1 or R2 may advantageously represent a C5~C-j cycloalkyl radical, optionally substituted with one or more customary groups in the field of amino acids and peptides. The substituents are notably selected from the group formed by halogens, OH, O- (Ci-C6 alkyl), O-(C6-Ci0-aryl) , Ci-C6 alkyl.
An R1 or R2 group may advantageously represent a phenyl, riaphtyl, 5- or 6-membered heteroaromatic or indole radical optionally substituted with one or more customary groups in the field of amino acids and peptides. The substituents are notably selected from the group formed by halogens, OH, 0-(d-C6 alkyl), 0-(C6-C10-aryl) , C^-Cg alkyl.
For obvious reasons of steric hindrances, R1 and R2 cannot represent a cyclic radical simultaneously. Cyclic radical means said CS-C7 cycloalkyl radical as well as said phenyl, naphtyl, 5- or 6-membered heteroaromatic or indole radical.
R1 and R2 may also form together a C5-C7 cycloalkyl radical optionally substituted with one or more customary groups in the field of amino acids and peptides. The groups are notably selected from the group formed by halogens, OH, 0-(Ci-C6 alkyl), C1--C6 alkyl, 0- (C6-C10-aryl) .
If R1 and R2 do not form together a C5-C7 cycloalkyl radical, advantageously at least one of the R1 and R2 groups, as defined earlier, represents a hydrogen atom.
In the compounds of formula II, the functional groups are advantageously protected with suitable protective groups.
According to an advantageous alternative of the invention, R3 represents methyl, ethyl, tertio-butyl, benzyl, allyl, 9-fluorenylmethyl. Indeed, although there are a large variety of urethanes which may be used as protective groups, only a few of these urethanes are widely used in peptide synthesis. T-butyloxycarbonyl (Boc), benzyloxycarbonyl(Cbz) and 9-f luorenylrnethyloxycarbonyl (Fmoc) may notably be mentioned. Accordingly, N-carboxyanhydrides of alpha amino acids protected with these substituents particularly are of interest.
With the method according to the present invention, it is possible to avoid the use of a tertiary amine, in a very large amount from 50 to 200% molar relatively to the engaged NCA molar amount. The tertiary amines used in the prior art, N-methylmorpholine and pyridine, actually pose many problems. Notably, their use involves working with high dilution,
formation of extraneous products, an additional separation step, difficult recycling and furthermore, it is very costly.
With the method according to the invention, it is also possible to notably reduce the reaction times which are henceforth less than 24 hours and advantageously of the order of 1 to 4 hours, whereas for methods from the prior art, reaction times vary from 30 hours to 5 days.
With the method according to the invention, it is possible to obtain UNCAs, the purity of which as measured by GPC (gas phase chromatography) is larger than 90%, advantageously larger than 95%, with a very satisfactory yield, larger than 60% by mass.
According to an advantageous alternative of the invention, the solvent is selected from the group consisting of cyclic or linear C4-Ci0 ethers, and chlorinated CI-GS alkanes. Advantageously, the solvent is THF (tetrahydrofurane).
When the solvent of the reaction is THF, the amount of introduced solvent is generally comprised between 500 g and 2 kg of solvent for one engaged mol of NCA with formula II.
According to an advantageous alternative of the method according to the present invention, the introduced amount of TBDA varies from 0.1 to 5% molar of TEDA, relatively to the engaged molar amount of NCA with formula II. Still more advantageously, the introduced amount of TEDA varies from 0.2% to 1% molar, relatively to the engaged molar amount of NCA with formula II.
The NCA with formula II is advantageously reacted with 1.1 to 1.5 equivalents of dicarbonate of formula III in the presence of TEDA, in particular in the presence of 0.2 to 1% molar of TEDA relatively to the engaged molar amount of NCA of formula II.
The dicarbonate of formula III is advantageously introduced, as a solution, into a portion of the solvent, advantageously in 0.5 and 2.0 parts by weight relatively to the total engaged weight amount of dicarbonate, into the reaction medium comprising the other required portion of
solvent, regularly, the NCA with formula II to be transformed arid TEDA. Upon introducing the dicarbonate, the temperature of the reaction medium is maintained between -20 and 5°C, advantageously between -15 and 5°C, even more advantageously between -10 arid 0°C. According to an advantageous alternative of the invention, the reaction takes place under an inert atmosphere.
The catalytic effect of TEDA allows a technique for gradually introducing dicarbonate into the reaction medium, with which exothermicity may be controlled by stopping the introduction, thereby avoiding any risks of a dangerous reaction runaway.
With the method, it is possible to work in a much more concentrated medium which, coupled with reduced reaction times, provides a substantial gain in productivity and greatly .limits risks of polymerization.
Upon completing the addition of dicarbonate of formula III, the reaction medium is advantageously left under stirring, for at least 30 minutes at a temperature between -5 and 10°C.
Upon completing the addition of dicarbonate of formula III, as soon as the reaction medium has optionally been left under stirring, the reaction medium is filtered, and then at least 80%, advantageously about 90%, of the solvent is removed by evaporation under reduced pressure. Next, a non-solvent compound is preferably added in an amount equivalent to the amount of reaction solvent removed by evaporation in order to cause precipitation of the UNCA of formula I which is then recovered by filtration, if necessary after removing said suitable protective groups.
According to one alternative of the invention, the solvent is removed by evaporation under reduced pressure at a temperature between 15 and 30°C, advantageously at room temperature.
The non-solvent compound of UNCA is advantageously a C5~ Cio linear or branched alkane, notably heptane B.
According to an alternative of the invention, the precipitate is then dried in vacua at a temperature less than 30°C.
The following examples illustrate the present invention and are non-limit ing.
Example 1: preparation of Boc-Val-NCA:
The acronym Val represents the alpha-amino acid: valine. Val-NCA therefore represents the compound of the following formula:
(Formula Removed)
itre reactor equipped with a cryostat, a funnel, a nitrogen flow system, mechanical stirring and a thermometer probe, 204 g of THF,
37.5 g (0.26 mol) of Val-NCA, 0.15 g (1.3 mmol) of TEDA
are introduced after inerting with nitrogen and cooling to -5 ± 2°C.
The reaction medium is stirred for Vz hour, and then a solution of 69 g (0.316 mol) of (Boc)2O in 50 g of THF is slowly introduced therein over 2 hours via a dropping funnel, while controlling the temperature to -5°C ± 2°C. Slight gas evolution occurs.
The reaction medium is maintained under stirring for 1 hour after completing the dropping of (Boc)20.
The reaction medium is filtered at 0°C on an inert mounted pre-layer with THF and the reactor is rinsed as well as the inert pre-layer with 50 ml of THF.
The filtrates are again placed in the double-jacketed reactor still under nitrogen and 300 ml of THF are distilled
at a reaction medium temperature from 18 to 26° C under a pressure from 140 to 160 mbar.
At a temperature of 25°C, 300 ml (215 g) of heptane B are added. The valine UNCA product precipitates. 300 ml of THF/heptane B mixture are then distilled at 25 °C and under 900-100 mbar until a volume of reaction medium of about 100 ml is obtained. 200 ml of heptane B are then added at a temperature of about 25°C. The reaction medium is cooled to -10°C, a temperature that is maintained for 1 hour. Filtration is performed on a sintered N°3 filter, under a nitrogen atmosphere at -10°C. The product is dried in a vacuum oven at a temperature of 25 ± 5°C.
51.6 g (yield 80.8%) of a white powder with rotatory power of 59.1° (C = 1, THF) are thereby obtained with a purity of 100% as measured by GPC.
Example 2: preparation of Boc-Ile-NCA:
The acronym He represents the alpha amino acid: isoleucine. Ile-NCS therefore represents the compound with the following formula:
(Formula Removed)
One proceeds as in Example 1 with:
- 200.9 g of THF,
- 40.0 g (0.255 mol) of Ile-NCA,
- 0.14 g (1.3 mmol) of TEDA,
and a solution of 66 g (0.302 mol) of (Boc)2O in 66 g of THF.
After filtration and drying, 51.5 g (yield 78.6%) of the expected product with a melting point of 107.6°C and with a rotatory power of 60.3° (C = 1, THF) are recovered. Purity as measured by GPC is 99.3%.
xample 3: Preparation of Boc-D-Phe-NCA:
The acronym Phe represents the alpha amino acid: phenylalanine. Phe-NCA therefore represents the compound with following formula:
(Figure Removed)
One proceeds as in Example 1 with the difference that the temperature is set to -17 ± 1°C with:
- 427 g of THF,
- 25 g (0.131 mol) of D-Phe-NCA,
- 0.07 g (0.65 mmol) of TEDA,
and a solution of 34.2g (0.157 mol) of (Boc)2O in 21.5g of THF.
After filtration and drying, 24 . 1 g (yield 63%) of the product in compliance with the expected 1HNMR structure, is recovered with 95.2% purity as measured by GPC.
Example 4 : _ Preparation of N-Ethoxycarbonyl-valine-N-
carboxyanhydrid_ (EtOC-Val-NCA)
In a double-jacketed 1 L reactor equipped with a cryothermostat, a dropping funnel, a nitrogen flow system, mechanical stirring and a thermometer probe,
- 204 g of THF,
- 20.0 g (0.141 mol) of Val-NCA,
- 0.078 g (0.7 mmol) of TEDA
are introduced, after inerting with nitrogen and cooling to -5 ± 2°C.
The reaction medium is stirred for 30 minutes, and then 27.1 g (0.167 mol) of diethyl dicarbonate [EtOC) 2O] are slowly introduced therein over 1 hour via the dropping funnel, while controlling the temperature to -5° ± 2°C. Slight gas evolution occurs.
The reaction medium is maintained under stirring for 1 hour after completion of dropping of (EtOC)2O.
THF is concentrated at a reaction medium temperature from 18 to 26°C under a pressure from 140 to 160 mbar.
220 ml of heptane B are added at a temperature of 25°C and poured into a large amount of ice-water. The product precipitates. Filtration is performed on a sintered N°3 filter under a nitrogen atmosphere. The product is dried in a vacuum oven at a temperature of 25 ± 5°C.
20.1 g (yield 67%) of a white powder is thereby obtained, the ^H NMR and 13C NMR spectra of which comply with the expected structure.















WE CLAIM:
1. A method for obtaining urethane-protected N-carboxyanhydrides of alpha aminoacids of formula I, using triethylene diamine
(Formula Removed)
wherein R1 and R2, are either identical or different, represent a hydrogen atom, a C1-C8 linear or branched alkyl radical, optionally substituted, a C5-C7 cycloalkyl radical, optionally substituted, a phenyl, napthyl, 5- or 6- membered heteroaromatic radical, or indole, optionally substituted, with the proviso that R1 and R2 do not each represent a cyclic radical simultaneously, or R1 or R2 form together a C5-C7 cycloalkyl radical optionally substituted, the optional substituents being conventional; R3 represents a saturated or unsaturated, linear or branched C1-C10 alkyl radical, or an aralkyl or alkylaryl radical with 7 to 14 carbon atoms, wherein a N-carboxyanhydride of alpha-amino acid of formula II,
(Formula Removed)
wherein R1 and R2 have the same meaning as for formula I, is reacted with at least one equivalent, relatively to the used molar amount of N-carboxyanhydride of alpha-amino acid of formula II, of a dicarbonate of formula III
(Formula Removed)
wherein R3 has the same meaning as for formula I, in the presence of 1,4-diazabicyclo[2.2.2]octane, also designated as triethylene diamine in an amount less than 5% molar relatively to the used molar amount of N-carboxyanhydride of alpha-amino
acid of formula II, in an organic solvent with a melting point less than about -20 °C,
the reaction being optionally followed by the following successive steps:
i) filtering the reaction medium;
ii) removing at least 80%, advantageously about 90%, of the solvent by evaporation
under reduced pressure; iii) adding a non-solvent compound, in an amount equivalent to the amount of
reaction solvent by evaporation, in order to precipitate the urethane-protected N-
carboxyanhydrides of alpha amino acid of formula I; and iv) recovering the urethane-protected N-carboxyanhydrides of alpha amino acid of
formula I by filtration.
2. The method as claimed in claim 1, wherein the optional substituents are selected from the group consisting of OH, SH, NH2, NHC(NH)NH2, CONH2, O-(C1-C6 aryl), S-(C1-C6 alkyl), COO-(C1-C6 alkyl), COO-(C5-C8 aralkyl).
3. The method as claimed in any of the preceding claims, wherein R3 represents methyl, ethyl, tertiobutyl, benzyl, allyl, or 9-fluorenyl-methyl.
4. The method as claimed in any of the preceding claims, wherein the solvent is selected from the group of C4-C10 ethers and chlorinated C1-C5 alkanes.
5. The method as claimed in claim 4, wherein the solvent is tetrahydrofuran.
6. The method as claimed in any of the preceding claims, wherein the amount of 1,4-diazabicyclo[2.2.2]octane varies from 0.1% to 5% molar, relatively to the used molar amount of N-carboxyanhydride of alpha-amino acid of formula II.
7. The method as claimed in claim 6, wherein the amount of l,4-diazabicyclo[2.2.2]octane varies from 0.2% to 1% molar, relatively to the used molar amount of N-carboxyanhydride of alpha-amino acid of formula II.
8. The method as claimed in any of the preceding claims, wherein the N-carboxyanhydride of alpha-amino acid of formula II is reacted with 1.1 to 1.5 equivalents of the dicarbonate of formula III, relative to the used molar amount of N-carboxyanhydride of alpha-amino acid of formula II.
9. The method as claimed in any of the preceding claims, wherein the dicarbonate of
formula III is introduced regularly as a solution in the reaction medium comprising
solvent, the N-carboxyanhydride of alpha-amino acid of formula II and 1,4-
diazabicyclo[2.2.2]octane.
10. The method as claimed in claim 9, wherein the temperature of the reaction medium is maintained, during introduction of the dicarbonate, between -20 and 5 °C, advantageously between -10 and 0 °C.
11. The method as claimed in any of claims 1 to 10, wherein the solvent is removed by evaporation under reduced pressure at a temperature between 15 and 30 °C.
12. The method as claimed in any of claims 1 or 11, wherein the non-solvent compound is a linear or branched C5-C10 alkane.
13. The method as claimed in any of claims 1 or 12, wherein the precipitate is then dried in vacuo at a temperature less than 30 °C.

Documents:

904-DELNP-2006-Abstract-(24-09-2010).pdf

904-delnp-2006-abstract.pdf

904-delnp-2006-Claims (19-11-2009).pdf

904-DELNP-2006-Claims-(08-09-2009).pdf

904-delnp-2006-Claims-(12-03-2010).pdf

904-DELNP-2006-Claims-(23-09-2009).pdf

904-DELNP-2006-Claims-(24-09-2010).pdf

904-delnp-2006-claims.pdf

904-DELNP-2006-Correspondence Others-(23-03-2011).pdf

904-delnp-2006-Correspondence-Others (19-11-2009).pdf

904-delnp-2006-correspondence-others 1.pdf

904-DELNP-2006-Correspondence-Others-(08-09-2009).pdf

904-DELNP-2006-Correspondence-Others-(10-05-2010).pdf

904-DELNP-2006-Correspondence-Others-(10-12-2008).pdf

904-delnp-2006-Correspondence-Others-(12-03-2010).pdf

904-DELNP-2006-Correspondence-Others-(14-09-2009).pdf

904-DELNP-2006-Correspondence-Others-(15-07-2009).pdf

904-DELNP-2006-Correspondence-Others-(23-09-2009).pdf

904-DELNP-2006-Correspondence-Others-(24-09-2010).pdf

904-DELNP-2006-Correspondence-Others-(30-12-2010).pdf

904-delnp-2006-correspondence-others.pdf

904-delnp-2006-description (complete).pdf

904-delnp-2006-form-1.pdf

904-delnp-2006-form-18.pdf

904-delnp-2006-form-2.pdf

904-delnp-2006-form-26.pdf

904-DELNP-2006-Form-27-(23-03-2011).pdf

904-DELNP-2006-Form-3-(30-12-2010).pdf

904-delnp-2006-form-3.pdf

904-delnp-2006-form-5.pdf

904-DELNP-2006-GPA-(14-09-2009).pdf

904-delnp-2006-pct-210.pdf

904-delnp-2006-pct-409.pdf

904-delnp-2006-pct-416.pdf


Patent Number 245195
Indian Patent Application Number 904/DELNP/2006
PG Journal Number 02/2011
Publication Date 14-Jan-2011
Grant Date 06-Jan-2011
Date of Filing 21-Feb-2006
Name of Patentee ISOCHEM
Applicant Address 12, QUAI HENRI IV, F-75004 PARIS, FRANCE.
Inventors:
# Inventor's Name Inventor's Address
1 GRIMA, JULIEN 189, RUE LA REPUBLIQUE, F-91150 ETAMPES, FRANCE.
2 LHERMITTE, HERVE 9, RUE FESSART, F-75019 PARIS, FRANCE.
3 PARIS, ANTOINE 101, RUE DU CHAMP DES OISEAUX, F-76000 ROUEN, FRANCE.
PCT International Classification Number C07D 263/44
PCT International Application Number PCT/FR2004/002148
PCT International Filing date 2004-08-17
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 0310101 2003-08-22 France