Title of Invention


Abstract The present invention relates to a Compounds of formula (1) ±3-(4-phenyl-l-piperazinyl)-l,2-propanediol cyclic acetals.wherein: each of Ra and Rb, which can be the same or different, is hydrogen, C1-C6-alkyl, phenyl; or Ra and Rb, taken together with the C atom they are linked to, form a 4- to 7-membered carbocyclic ring.
The present invention relates to (±) 3-(4-phenyl-l-piperazinyl)-l,2-propanediol cyclic acetals, a process for their optical resolution and the use thereof as intermediates for the preparation of (-)3-(4-phenyl-l-piperazinyl)-1,2-propanediol (levodropropizine) and the salts thereof.
More precisely, the invention relates to (±) 2,2-substituted-l,3-dioxolanes of formula (1):
(Formula Removed)
each of Ra and Rb, which can be the same or different, is hydrogen, C1-C6-alkyl, phenyl, or
Ra and Rb taken together with the C atom they are linked to, form an optionally substituted 4- to 7- membered carbocyclic ring.
Advantageously, in the compounds of the invention of formula (1), Ra and Rb are alkyl groups containing less than 6 C atoms. Ra and Rb are preferably the same; more preferably, Ra and Rb are methyl or ethyl or; together with the C atom they are linked to, form a ring containing 5 to 6 carbon atoms.
The invention also relates to the enantiomerically pure monobasic salis of the 2,2-substituted-l,3-dioxolanes of formula (1) with jpharmaceutically acceptable chiral acids such as L-malic, D- and L-tartaric, D- and L-mandelic, L- and D-camphorsulfonic acids.
Examples of particularly preferred compounds of the invention are:
(±) l,2-cyclopentylidene-3-(4-phenyl-piperazin-l-yl)-propane-l,2-diol;
(±) l,2-cyclohexyliden-3-(4-phenylpiperazin-l-yl)-propane-l,2-diol;
(±) l,2-(2-propylidene)-3-(4-phenyl-piperazin-l-yl)-propane-l32-diol;
(±) lJ2-(3-pentyliden)-3-(4-phenyl-piperazin-l-yl)-propane-lJ2-diol;
S(-)-l,2-cyclohexyliden-3-(4-phenylpiperazin-l-yl)-propane-l,2-diol L-tartrate;
S(-)-l,2-cyclopentylidene-3-(4-phenylpiperazin-l-yl)-propane-l,2-diol L-tartrate.
The compounds of the invention of formula (1) are prepared by reacting phenylpiperazine with a (±)l,2-glyceryl-dioxolane of formula (2):
(Formula Removed)
wherein X is selected from the group consisting of Cl, Br, I and a suitable sulfonic ester (R-SO3-), R being selected from the group consisting of C1-C3-alkyl (preferably methyl), trifluoromethyl, phenyl, p-tolyl and p-methoxy phenyl.
Dioxolanes cf formula (2) are known compounds and/or can be prepared according to known methods.
The sulfonic esters of formula (2) (X= R-SO3-) are prepared by esterification with an anhydride or with an alkyl- and/or aryl-sulfonic acid chloride of formula (3)
R-SO3H (3)
of a (±) 2,2-substituted-l,3-dioxolane-4-methanol of formula (4):
(Formula Removed)
wherein Ra and Rb have the meanings defined above.
Compounds of formula (4) are known compounds. Racemates of formula (4) are, in fact, used as substrates for fermentative resolution processes [US Pat. 5.190.867 (02.03.1993)]. The preparation of dl-isopropylideneglycerol (Merck 12.5232; Beil 19.65) starting from glycerol is described in Org. Synth. Coll. Vol III.
The 4-halomethyl-dioxolanes of formula (2) wherein X is Cl, Br or I can be prepared starting from the corresponding sulfonates of formula (2) (X = RSO3- wherein R is as defined above) by reaction with an alkali or alkaline-earth halide in an inert solvent, selected from the group consisting of acetone, methyl ethyl ketone, tetrahydrofuran, dioxane, dimethylsulfoxide, acetonitrile, a C1-C4-alcohol and mixtures thereof. A preferred method for the synthesis of said compounds comprises the dioxolanation of the corresponding 3-halo-l,2-propanediols, as disclosed in EP 0930311 (21.07.1999). Particularly preferred is 3-chloro-propane-1,2-diol; preferred acetalyzing agents are formaldehyde, acetaldehyde and benzaldehyde, aceione, diethyl ketone, benzophenone, cyclohexanone, the acetals and/or enol ethers thereof, such as 2,2-dimethoxypropane, 2,2-dimethoxyethane, 2-methoxy-propene.
Alternatively, dioxolanes of formula (2) (X = Cl or Br) can also be obtained by acetalyzation of epichlorohydrins or epibromohydrins with a cycloalkanone according to the processes described for the preparation of (+)2-chloromethyl-l,4-dioxaspiro[4,5]-decane in FR 1522153 or by Blicke FF et al., J. A. C. S, 74, 1735 (1972) and ibidem, 76, 1226 (1954).
The alkylation reaction of phenylpiperazine with a 1,2-glyceryl-dioxolane of formula (2) is performed using reaction conditions conventionally used for the conversion of a secondary amine to a tertiary amine, using for each mol of the alkylating agent of fonnula (2) at least one mol or a slight molar excess of phenylpiperazine in the presence of at least one mol of a counterbase. The counterbase is used in at least equimolar amounts with respect to the alkylating agent of formula (2), and is selected from the group consisting of finely divided inorganic bases such as alkali or alkaline-earth (Na, K, Mg, Ca) carbonates or bicarbonates or Ca or Mg oxides, or tertiary amines as triethylamine, dimethyl or diethylaniline, aromatic amines as pyridine, picoline and collidine and, if desired, the phenylpiperazine itself which may be subsequently recycled to a subsequent production cycle.
The alkylation reaction can be performed in the hot, optionally in the presence of inert solvents such as toluene or xylene which, when operating under reflux of the solvent, will advantageously reduce the reaction times.
After completion of the alkylation reaction, any insolubles are filtered or centrifuged off, then the organic phases are repeatedly washed with water to easily remove the impurities and side-products, and the solvent is distilled off to obtain in high yields a residue consisting of a substantially pure 1,3-dioxolane of the invention of formula (1), which is recovered either by direct crystallization or after salification with a molar equivalent of a carboxylic acid.
Compounds (1) and the salts thereof are suprisingly easy to crystallize from the usual solvents: the process of the invention therefore minimizes any risks of comaminations due to the presence of glycidols and/or epihalohydrins traces as potential impurities.
The monobasic salts of the compounds of formula (1) ere obtained by using conventional methods such as salification with equimolecdar amounts of the desired acid in a suitable solvent and subsequent crystallization of the
resulting salt.
It has been found that the salification of compounds (1) with chiral acids, particularly with L-tartaric acid, is an efficient optical resolution method to recover the S-enantiomers of compounds (1) in high yields.
Said (S)(-) enantiomers and the salts thereof are useful as antitussive agents or as intermediates for the synthesis.of.(-) 3-(4-phenyl-l-piperazinyl)-1,2-propanediol (levodropropizine) by hot hydrolysis of aqueous solutions of the (-)l,3-dioxolanes of formula (1) catalyzed by a molar excess of a diluted aqueous solution of a mineral acid such as hydrochloric acid, or of a carboxylic acid such as acetic, malonic or citric acids.
The following examples further illustrate the invention.
Example 1
A solution of 11.98 g of (±) 2,3-0-(3-pentylidene)-glycerol tosylate in n-butanol (70 mL) is added under strong stirring with 4.5 g of finely divided sodium carbonate, then with 6 ml of phenylpiperazine. The mixture is refluxed under stirring and reacted at this temperature for 20 h. Butanol is then evaporated off under reduced pressure, the residue is taken up with water and repeatedly extracted with ethyl acetate. The combined organic phases are dried and filtered, then the solvent is evaporated off under vacuum and the resulting residue is crystallized from aqueous methanol to obtain 8.95 g of (±) 1.2-(3-pentyliden)-3-(4-phenyl-piperazm-l-yi)-propane-l,2-diol, also named 4-phenylpiperazine,1-(2,2-diethyl-l,3-dioxolan-4-yl-methyl).
Example 2
Following ths procedure described in Example 1, using (±) 2,3-0-(2-propylidene)-glyce:ol tosylate, (±) l,2-(2-propylidene)-3-(4-phenyl-piperazin-l-yl)-propane-l,2-col, also named 4-phenylpiperazine,l-(2,2-dimethyl-l-3-diossolan-4-yl-met:yl), is obtained.
Example 3
A solution of 3.28 g of (±) l,4-dioxaspiro-[4.5]decane-2-methyl chloride in toluene (16 mL) is added under inert gas atmosphere with 5,45 ml of phenylpiperazine. The mixture is then refluxed to complete the reaction (approximately 8 hours). The reaction mixture is cooled to about 50°C, added with 10 ml of water and kept under strong stirring for at least 10 minutes. The phases are separated, and the organic phase is repeatedly washed with water. The solvent is evaporated off to obtain a thick oil which is dissolved in hot isopropanol (15 mL). The resulting solution is slowly cooled to separate (±) 1,2-cyclohexyliden-3-(4-phenylpiperazin- l-yl)propane-l ,2-diol as a crystalline solid, m.p. 58-61°C.
lH NMR 8 7.26 (t, 2H, J=7.4Hz); δ 6.95 (d, 2H, J=8.9Hz); 5 6.85 (t, 1H, J=7.28Hz); 8 4.3 (m, 1H, J=6.1Hz); 8 4.1 (dd, 1H, J,=8.0Hz, J2=6.1Hz); 5 3.65 (dd, 1H? J1=8.0, J2=7.1Hz); 8 3.2 (t, 4H, J=5.05Hz); 5 2.9÷2.55 (m, 6H); 5 1.7-1.3(m, 10H)
Example 4
Following the procedure described in Examples 1 and 3. by reacting a 1.3-dioxolane selected from the group consisting of:
(±) l,4-dioxaspiro[4.4]nonane-2-methanol, mesylate;
(±) 1,4-dioxaspiro[4.4]nonane-2-methyl thloride;
(+) l,4-dioxaspiro[4.5]decane-2-methanol, trifluoromethanesulfonate;
(±) l,4-dioxaspiro-[4.5]decane-2-methanol, mesylate;
(±) 1 ?3-dioxoiane-4-chloromethyI-2,2-dimethyl;
(±) 1.3-dioxoiane-4-bromo-methyl-2.2-dimethyl;
with phenylpiperazine, the following compounds were obtained:
(±) l;2-cyclopentylidene-3-(4-phenyl-piperazin-l-yl)-propane-l,2-diol;
(i) l,2-cyclohexylidene-3-(4-phenyl-piperazin-l-yl)propane-l,2-diol;
(±) l,2-(2-prcpylidene)-3-(4-phenyl-piperazin-l-yl)-propaae-l,2-diol
Example 5
3 g of L-tartaric acid are added under stirring to a solution of (±) 1,2-cyclohexylidene-3-(4-phenyl-piperazin-l-yl)propane-l,2-diol (6.32 g) in absolute ethanol (70 ml). The mixture is stirred until complete dissolution, then cooled to 5-S°C. 4.05 g of a crystalline solid are obtained, which is Tecrystallized from absolute ethanol to yield 3.82 g of S(-)-l,2-cyclohexylidene-3-(4-phenyl-piperazin-l-yl)propane-l,2-diol L-tartrate m.p. 131 - 132°C, [α]D - -9.6° (c=l%; rneoh);
1H NMR 6 7.5 (dd, 2H, J1=8.7 J2=7.3); 5 7.2 (m, 3H); 8 4.8 (m, 1H); 8 4.4 (s, 2H); 5 4.35 (dd, 1H, J1=8.9Hz J2=6.7Hz); 5 3.86 (dd, 1H, J1=8.9Hz J2=5.8Hz); 8 3.6-3.4 (m, 10H); 8 1.8-1.48 (m, 10H).
Example 6
A solution of 2.4 g of S(-)-l,2-cyclohexylidene-3-(4-phenyl-piperazin-l-yl)propane-l,2-diol L-tartrate in water is alkalinized (pH =8) by addition of 2N NaOH, then exhaustively extracted with ethyl acetate. The combined organic phases are washed with a 10% monobasic sodium phosphate solution, dried over sodium sulfate and evaporated to dryness, then crystallized from isopropanol to obtain 1.5 g of S(-)-l,2-cyclohexylidene-3-(4-phenyl-piperazin-l-yl)propane-l,2-diol, m.p. 63-64°C, [α]D=- 7.8°(1% MeOH).
lH NMR 57.26 (t, 2H, J=7.4Hz); 8 6.95 (d, 2H, J=8.9Hz); 8 6.85 (t, 1H, J=7.28Hz); S 4.3 (m, 1H, J=6.1Hz); 8 4.1 (dd, 1H, J1=S.OHz, J2=6.1Hz); 8 3.65 (dd, 1H, J1=8.0, J2=7.1Hz); 5 3.2 (t, 4H, J=5.05Hz); 8 2.9-2.55 (m, 6H): δ l.7÷1.3(m, 10H).
Example 7
By salification of a 1,3-dioxolane prepared according to the processes of Examples 1-4 with L-tartaric acid and fractional recrystallization of the resulting salts, the following compounds were prepared:
S(-)-1,2-(2-propylidene)-3-(4-phenyl-piperazin-1 -yl)-propane-1,2-diol,
which where subsequently neutralized according to the process of Example 6, to obtain the corresponding free bases.
Example 8
A suspension of 2.8 g of S(-)-l,2-cyclopentylidene-3-(4-phenyl-piperazin-l-yl)-propane-l,2-diol in 70 ml of aqueous acetic acid (10% w/v) is refluxed for 2h, then vapor is bubbled therein to distil off cyclopentanone, which is separated. The aqueous phase is neutralized to pH 7.5 by addition of a 10% NaOH solution, then cooled to 5-10°C, to obtain 1.97 g of (-) 3-(4-phenyl-piperazin-l-yl)-propanediol m.p. 102-103°C, [α]D= -23.5° (2.8% CH2C12).
Example 9
Alternatively. 0.35 molar equivalents of one of the 1,3-dioxolane
derivatives described in Examples 6 and 7, S(-) 1,2-(2-propylidene)-3-(4-
phenyl-piperazin-1 -yl)-propane-1,2-diol; S(-) 1,2-cyclopentylidene)-3-(4-
phenyl-piperazin-1 -yl)-propane-1,2-diol; S(-)-1,2-cyclohexylidene-3-(4-
phenyl-piperazin-1 -yl)propane-1,2-diol; are added in portions to a 36% hydrochloric acid solution (36 mL) in 45 ml of water under stirring; the suspension is heated to 80°C to obtain a clear solution, which is kept at this temperature for a further 30 minutes, then cooled to 20-25°C and the aqueous phase is repeatedly is extracted with dichloromethane (3x15 ml), then added with n-butanol (0.5 1). The diphasic mixture is refluxed to distil the water n-butanol azeotrope, recovering about 300 ml of distillate, then cooled to promote the cnrtallization of (-)3-(4-phenyl-piperazin-l-yl)-propanedioI hydrochloride (85g).
A solution of the hydrochloride in 125 ml of water is decolorized by heating at 50°C with active charcoal (2.2 g) for 15 minutes, filtered then neutralized by addition of an ammonium hydroxide aqueous solution (30% w/w). After briefly heating to 50°C, crystallization is started by addition of (-) 3-(4-phenyl-piperazin-l-yl)-propanediol crystals. The suspension is left to spontaneously cool, then kept for 2 hours at +2 - +4°C, finally filtered to yield 70-72 g of (-) 3-(4-phenyl-piperazin-l-yl)-l,2-propanediol.

1. Compounds of formula (1) ±3-(4-phenyl-1 -piperazinyl)-1,2-
propanediol cyclic acetals.
(Formula Removed)
each of Ra and Rb, which can be the same or different, is hydrogen, C1-C6-alkyl, phenyl; or
Ra and Rb, taken together with the C atom they are linked to, form a 4- to 7-membered carbocyclic ring.
2. Compounds as claimed in claim 1 wherein Ra and Rb are the same.
3. Compounds as claimed in claim 1 or 2 wherein Ra and Rb are methyl or ethyl.
4. Compounds as claimed in claim 1, selected from:
(±) 1,2-cyclopentylidene-3-(4-phenyl-piperazin-1 -yl)-propane-1,2-diol;
(±) l,2-cyclohexylidene-3-(4-phenyl-piperazin-l-yl)-propane-l,2-diol;
(±) l,2-(2-propylidene)-3-(4-phenyl-piperazin-l-yl)-propane-l,2-diol;
(±) 1,2-(3-pentylidene)-3-(4-phenyl-piperazin-1 -yl)-propane-1,2-diol;
S(-)-1,2-Cyclohexylidene-3-(4-phenyl-piperazin-1 -yl)-propane-1,2-diol L-tartrate;
S(-)-1,2-Cyclopentylidene-3-(4-phenyl-piperazin-1 -yl)-propane-1,2-diol L-
5. A Process for the preparation of the compounds of formula (1) as claimed
in claims 1 to 4 by reacting phenylpiperazine with a (±) 1,2-glyceryl-
dioxolane of formula (2):
(Formula Removed)
Wherein X is selected from the group consiting of Cl, Br, I and a suitable sulfonic ester (R-SO3-), R being selected from the group consiting of C1-C3-alkyl, (preferably methyl), trifluoromethyl, phenyl, p-tolyl and p-methoxy phenyl wherein the reaction is carried out in the presence of a base using toluene or xylene as solvents..
6. A process as claimed in claim 1, wherein the mineral acid is hydrochloric acetic, malonic or citric acid.
7. Compound of formula (1) substantially as herein described with reference to the foregoing description, tables and the accompanying examples.
8. A process for the preparation of the compounds of formula (1) substantially as herein described with reference to the foregoing description tables and the accompanying examples.





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Patent Number 244536
Indian Patent Application Number 63/DELNP/2003
PG Journal Number 51/2010
Publication Date 17-Dec-2010
Grant Date 09-Dec-2010
Date of Filing 17-Jan-2003
Name of Patentee DOMPE' S.P.A
Applicant Address VIA CAMPO DI PILE, I-67100 L'AQUILA, ITALY
# Inventor's Name Inventor's Address
PCT International Classification Number C07D 317/28
PCT International Application Number PCT/EP2001/08305
PCT International Filing date 2001-07-18
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 MI2000A001735 2000-07-28 Italy