Title of Invention	ANTIBODIES SPECIFICALLY BINDING HUMAN IgG-FC RECEPTOR RIIa (FcyRIIa and ARTIFICAL PEPTIDE OR POLYPEPTIDE COMPRISING A CDE OF FcyRIIa
Abstract	The invention relates to novel immunogens carrying conformationally discriminating epitopes (CDEs) and to immunization methods for producing antibodies that specifically recognize proteins with very closely related homologues In particular, the invention relates to antibodies which are specific for either FcyRllb or FcyRlla

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WO 2005/051999 PCT/EP2004/013450 Substance binding human IgG Fc receptor lib (FcyRHb) Description The invention relates to novel immunogens carrying conformationally discriminating epitopes (CDEs) and to immunization methods for producing antibodies that specifically recognize proteins with very closely related homologues. In particular, the invention relates to antibodies which are specific for either FcyRllb or FcyRlla and which are useful for the diagnosis and treatment of autoimmune diseases, infections, tumors and other conditions where the immune system is involved. Fc receptors (FcRs) play a key role in defending the human organism against infections. After pathogens have gained access to the blood circulation they are opsonized by antibodies (immunoglobulins, Igs). This leads to the formation of immune complexes. The Fc portions of the antibodies can bind to Fc receptors which are present on virtually all cells of the immune system. Specific FcRs exist for all Ig classes. The Greek letter indicates the Ig class to which it binds, i.e. Fey receptors recognize IgG etc. It has been known for a number of years that the Fc receptors for IgG (FcyR) play an important role in triggering effector responses (Metzger, 1992A). These include, depending on the expressed FcyR and cell type, endo- and phagocytosis resulting in neutralization of the pathogens and antigen presentation, antibody-dependent cell-mediated cytotoxicity (ADCC), neutrophil activation, regulation of the antibody production or the secretion of inflammatory mediators (Fridman et a!., 1992; van de Winkel and Capel, 1993; Ravetch and Bolland, 2001). In contrast to the beneficial role FcRs play in the healthy individual, they also transmit the stimulation of the immune system in allergies (e.g. mediated by FceRIa) or autoimmune diseases. Moreover, some viruses WO 2005/051999 PCT/EP2004/013450 -2- employ FcyRs to get access to cells like HIV (Homsy et al., 1989) and Dengue (Littaua et al., 1990) or slow down the immune response by blocking FcyRs as in the case of Ebola (Yang et al., 1998) and Measles (Ravanel et al., 1997). Fc receptors for IgG (FcyR) are the most widespread of the Fc receptor family and are expressed in a defined pattern on all immunological active cells. FcyRI is constitutively expressed on monocytes and macrophages and can be induced on neutrophils and eosinophifs. The physiological role of FcyRI is still unknown as the expression on monocytes is not vital (Ceuppens et al., 1988). The glycosylphosphatidylinositol-anchored form (GPl) of FcyRIII (FcyRlllb) is exclusively expressed on granulocytes. Due to its missing cytoplasmic part, the signal transduction into the cell occurs solely via other transmembrane proteins like complement receptor type 3 (CR3) that can at least associate with FcyRlllb (Zhou et al., 1993; Poo et al., 1995). FcyRllla is mainly expressed on monocytes and macrophages but only in conjunction with an associated protein called y-chain. FcyRlla is the receptor with the widest distribution on immune competent cells and is mainly involved in the endocytosis of immune complexes. FcyRllb is expressed on B cells where it is the only IgG receptor, and on effector cells such as macrophages, neutrophils and mast cells, but not on NK cells and T cells. Structurally, the extracellular part of the FcyRs consists of three (FcyRI, CD64) or two (FCERI, FcyRIl, CD32 and FcyRIII, CD16) Ig-like domains (ca. 10 kDa/domain) and therefore belong to the immunoglobulin super family. In addition to the extracellular domains, FcRs have a transmembrane domain, and an intracellular domain with the exception of the GPI-anchored FcyRlllb. The receptors are homologous to each other, and the overall identity in amino acid sequence among the FcyRs and the FceRIa exceeds 40% in their extracellular regions. FcyRila and FcyRllb differ in their extracellular region WO 2005/051999 PCT/EP2004/013450 -3- by only 6% of the amino acid residues. Nevertheless, both forms can be distinguished by their binding characteristics to human and mouse IgG subclasses (van de Winkel and Capel, 1993) and their differing affinity to human IgGs (Sondermann et al., 1999A). FcRs are highly glycosylated. The cDNA sequence of many Fc receptors is known, and some soluble recombinant FcR have been generated. Soluble recombinant Fc receptors which are characterised by an absence of transmembrane domains, signal peptide and glycosylation are disclosed in WO 00/32767. FcyRs occur in various isoforms (FcyRIa, b1, b2, c; FcyRlla 1-2, b1-3, c) and alleles (FcyRlla1-HR, -LR; FcyRlllb-NAI, -NA2) (van de Winkel and Capel, 1993). In contrast to the overall homologous extracellular parts, the membrane spanning and the cytoplasmic domains of up to 8 kDa large differ. The FcyRs can be divided into two general classes according to their function which may be an activating or an inhibitory one. The activating receptors are associated with a cytoplasmic 16 amino acid immunoreceptor tyrosine-based activation motif (ITAM) having the consensus sequence Y-X2-L/I-Xs-Y-Xz-L/I (Isakov, 1997). This motif can be found, for example in FcyRlla. The other class of FcRs are inhibitory receptors which have a cytoplasmic 6 amino acid inhibitory motif (ITIM) having the consensus sequence V/l-X-Y-X2-V/L (Isakov, 1997). An example of such an inhibitory FcR is FcyRllb. Activation and inhibition via the ITAM and ITIM motifs is effected by tyrosine phosphorylation. Depending on the particular cell type, activated by the Fc receptor, different tyrosine kinases are involved in these signaling pathways (Amigorena et al., 1992). Both activating and inhibiting FcyRs may be expressed on the same cell which allows functioning of activation and inhibitory receptors in concert for a fine tuning of the immune response. WO 2005/051999 PCT/EP2004/013450 -4- FcyRllb has two inhibitory activities. One of these is dependent on the ITIM motif and occurs when FcyRlIb is ligated to an ITAM-carrying receptor (e.g. FcyRlla) resulting in the inhibition of ITAM-triggered calcium mobilization and cellular proliferation. This means that calcium-dependent processes such as degranulation, phagocytosis, ADCC, cytokine release and pro-inflammatory activation, and also B cell proliferation are blocked by FcyRlIb. The second inhibitory activity of FcyRllb involves homo-aggregation of the receptor (FcyRlIb clustering) which delivers a pro-apoptotic signal into the cytoplasm. The pro-apoptotic signal has only been reported in B cells and can be blocked by ligation of FcyRlIb to the B cell receptor (BCR). In vivo studies suggest that FcyRlfb plays a role in peripheral tolerance because FcyRllb-knockout mice develop spontaneously autoimmune diseases. On the other hand, FcyRlIb has also been reported to down-regulate cytotoxicity against tumors (Clynes et al., 2000). Mice deficient in FcyRlIb and treated with an anti-tumor antibody showed enhanced ADCC resulting in a reduction of tumor metastasis, whereas mice deficient in activating Fc receptors were unable to arrest tumor growth, when treated with the same antibody. The generation of antibodies by immunising animals with proteins or peptides as immunogens is known in the art. Conventional immunisation protocols use linear peptides as immunogens which are derived from antigens of interest. The disadvantage of such methods is that because the three-dimensional structure of the epitopes is often completely lost, the resulting antibodies are not very specific or they comprise a large fraction of antibodies directed to epitopes other than the one of interest. During the last decade, immunization protocols using Fc-receptor expressing cells or denatured Fc-receptors have only resulted in antibodies that were able to specifically detect denatured Fc-Receptors (Western Blot) or were not able to discriminate between the related FcyRNa and FcyRllb on cell WO 2005/051999 PCT/EP2004/013450 -5- fines (e.g. U-937, Raji) or blood cells. To date, there are no antibodies or other binding substances which bind selectively and specifically to FcyRllb in its native conformation and/or its natural environment. Conventional immunization protocols involving peptides. or recombinant proteins as antigens are not well suited to produce specific antibodies against proteins for which homologues with very high sequence identity exist. In general, antibodies are raised using small linear peptides as immunogens. Such peptides do not represent the native conformation of the epitope on the protein from which they are derived. In addition, the large majority of the antibodies produced by the immunized animal are directed against epitopes of the carrier protein to which the antigen is conjugated or against epitopes on the recombinant antigen that are common to the homologues. In consequence, the produced antibodies are not specific and/or fail to detect the antigen in its native conformation. Furthermore, glycosylation sites might be located within the epitopes of interest and mask these sites. Conventional immunization protocols which use these epitopes without the respective native glycosylation found in the target molecule result in antibodies that fail to recognize the antigen in its native conformation. One object of the present invention is to provide recombinant peptides or polypeptides which can be used as immunogens to raise antibodies capable of discriminating between an antigen of interest and closely related antigens, and a method of generating such peptides and the corresponding antibodies and other substances having immunological specificity. It is a further object of the present invention to provide substances which can selectively bind to and discriminate between Fc receptor subtypes, thereby acting as an Fc receptor binding substance useful for the treatment and diagnosis of immune disorders, in particular autoimmune diseases, and as anti-tumor agents which enhance the efficiency of such therapies by promoting ADCC against tumor cells. WO 2005/051999 PCT/EP2004/013450 -6- It is a further object of the present invention to provide an immunization protocol that will allow the generation of such FcyRllb-binding substances, in particular antibodies, especially monoclonal antibodies with the above-mentioned specificity. The inventors of the present invention have found a novel and inventive approach to developing substances, in particular antibodies, that are capable of discriminating between very closely related proteins and/or proteins and antigens with high homology. Surprisingly, it was found that it is possible to raise specific antibodies against proteins of interest when so-called conformationally discriminating epitopes (CDEs) are used as the antigen to which the antibodies are raised. The present invention therefore relates to an artificial peptide or polypeptide comprising a conformationally discriminating epitope (CDE) in its native conformation, wherein the CDE is structurally stabilized by circularization. For the purposes of the present invention, an artificial peptide or polypeptide is one that is produced by any technical- process such as recombinant techniques or preferably by peptide synthesis. A CDE is an epitope in a protein which has a specific conformation in the protein. Antibodies which are specific for such an epitope can discriminate between a protein and very closely related homologues. The CDE comprises at least one amino acid which differs between the protein in which it is present and the homologues of that protein (unique residue). The unique residues need not be in close proximity in the linear sequence of the protein in order to be part of the same epitope. The advantage of the present invention is that the peptides of the invention do not just have the linear sequence of those epitopes but mimic also their structure. The CDE contains at least one of such unique residues, preferably at least two, more WO 2005/051999 PCT/EP2004/013450 -7- preferably more than two of such unique residues. The CDE represents the binding site for an antibody. The peptides of the present invention preferably have a length of from 5, more preferably from about 8, more preferably from about 10 to about 30, more preferably to about 20, more preferably to about 18, more preferably to about 15 amino acids. Structural stabilization in this context means that the peptide is stabilized so that the CDE is present in as close to its native three-dimensional conformation in the original protein as possible. Structural stabilization can be achieved by a number of means. In particular, the peptide is circularized so that it forms a stable three-dimensional structure such as a loop. Stabilizing the peptide can be achieved by N- to C-terminal coupling, the formation of cysteine bridges or by bridging amino acid side chains. Pseudopeptides can be formed. Preferably, the peptide or polypeptide of the present invention also carries glycosylation moieties. The peptide is preferably generated so that glycosylated amino acids are incorporated at the same sites which are glycosylated in the native protein from which the CDE is derived. Preferably, the glycosylated amino acids are selected from N-acetyl-glucosamine, fucose, xylose, mannose, and galactose conjugates but this list is not exhaustive. If the discriminating epitope contains a N-glycosylation site, an artificial conjugate of an asparagine residue with a N-acetyl-glucosamine may be incorporated into the peptide, to enhance the probability that the natively glycosylated substrate is recognized by the resulting antibodies after successful immunization. Accordingly for O-glycosylation sites, a serine or threonine residue may be conjugated with a mannose, fucose, xylose, galactose or N-Acetyl-galactosamme residue respectively.- The peptides and polypeptides of the invention may additionally be coupled to a carrier molecule. Such carrier molecules are preferably selected from WO 2005/051999 PCT/EP2004/013450 -8- haptens, peptides, polypeptides and other immunogens. The peptides of the invention may be grafted onto other peptides and proteins, even the same protein or parts thereof from which the CDE was derived. A preferred embodiment of the invention is a pepiide carrying a CDE from an Fc receptor. The inventors of the present invention surprisingly found that there are specific epitopes on the extracellular portion of FcyRllb which allow the generation of antibodies which bind specifically to FcyRllb. This is particularly useful because the family of Fc receptors comprises unusually closely related homologues which are difficult to distinguish using conventional antibodies. In particular, the present invention makes it possible to generate substances which bind to FcyRllb but not to FcyRlla and vice versa. Similarly, the epitopes can be chosen so that FcyRlla is specifically recognized. In particular, the peptides or polypeptides according to the present invention comprise an epitope comprising at least one, preferably at least 2, preferably at least 3 of the following amino acids of the amino acid sequence of human FcyRllb according to Figure 1 and SEQ ID NO: 2: Gin 12, Arg27, Thr29, His30, Val104, Lys127, Ser132, Asn135, Tyr160, and Ala171, or the corresponding amino acids of FcyRlla according to SEQ ID NO: 1. More preferably, the epitopes useful for the purposes of the present invention comprise amino acids 27 to 30, and/or 127 to 135, and/or 160 to 171 of the amino acid sequence of FcyRllb (Figure 1, SEQ ID NO: 2), or the corresponding amino acids of FcyRlla (Figure 1). These peptides can represent FcyRlib-specific conformationally discriminating epitopes (CDEs), when structurally stabilized by circularization. in an adequate way as exemplified in Figure 2. Also, the corresponding epitopes of FcyRlla may be used to produce antibodies that specifically bind only to FcyRlla. WO 2005/051999 PCT7EP2004/013450 -9- Especially peptides comprising the amino acid sequence 127-KKFSRSDPN-135 and flanking amino acids are preferred because these peptides represent a FcyRllb-specific conformational epitope within the binding region of the Fc-Receptor to the Fc-fragment (WO 00/32767, Sondermann et al., 2000; Sondermann et al., 2001). Furthermore peptides containing the amino acid sequence 28-RGTH-31 and flanking residues are preferred because they represent a binding epitope apart from the binding region to the Fc-fragment. Moreover, this epitope may be further adapted to the native structure by circularisation and incorporation of a glycosylated asparagine residue at position 135. Thus, a preferred embodiment of the present invention is a peptide or polypeptide carrying the CDE according SEQ ID NO: 3. Preferably, the asparagine of position 135 (according to SEQ ID NO: 2) is glycosylated with N-acetyl-glucosamine. Preferably, the peptide is as shown in Figure 7, being circularized by linking the first and last amino acids in the sequence as shown in Figure 7. These artificial peptides can then be used directly for the immunization of animals or may be coupled to a carrier protein such as haptens or peptides or polypeptides, or ideally to the target protein itself. In a preferred embodiment, a CDE from FcyRllb or FcyRlla or a peptide carrying such a CDE is conjugated to FcyRllb or FcyRlla. The peptides and polypeptides of the present invention are preferably used as immunogens to immunize animals in order to generate specific antibodies and, with the aid of the sequence of those specific antibodies, further immunologically specific substances. The CDEs and the peptides carrying them may be used for the generation of immunomodulatory substances specifically recognizing the CDE. This is particularly preferred when CDEs of Fc receptors are chosen because they allow the generation of antibodies specific for individual members of the family of homologues. In particular, antibodies and other immunomodulatory substance recognizing either WO 2005/051999 PCT/EP2004/013450 -10- FcyRllb or FcyRIla but not both at the same time, can be generated. The present invention allows the generation of antibodies which are capable not only of specifically recognizing FcYRIIb or FcyRlla and discriminating between the two Fc receptors but also of doing so when the Fc receptors are in their natural environment, for example in cell culture or in vivo, e.g. in the blood stream. By coupling of the CDE to the protein from which it was derived, the background immune reaction against the carrier protein is reduced. The artificial modification by the covalently coupled CDE results in an increased immune response. This is especially important if the immunized animal expresses similar proteins which would be tolerated by its immune system. Thus, if the peptide of the invention carries a CDE of FcyRllb or FcyRIIa. it is preferably coupled to the respective Fc receptor itself. This increases the antigenicity. The coupling can occur by chemical linkage or other suitable means. This method preferably produces an immunogen with a high density of CDEs thereby presenting them in a different thus immunogenic environment. The initial immune response is directed against the targeted region (CDE) which produces antibodies that crossreact with the native structure to which they are coupled. These crossreacting antibodies mature towards higher affinity also recognizing the CDE in its natural environment. The peptides of the invention and the CDEs may also be used in screening of molecular libraries for binding molecules (e.g. peptides, organic molecules, peptidometics etc.) or genetically encoded libraries (e.g phage display of antibody variable domains or other frameworks like lipocalines) to find specifically binding substances to FcyRlib (or to FcyRlla). The peptides may be used to screen libraries of molecules binding specifically to either FcyRlla or FcyRllb on human cells. WO 2005/051999 PCT/EP2004/013450 -11 - Similar peptides according to the invention can be extracted from the structure of other proteins, e.g. receptors, that are related to each other but which have different functions (e.g. human FcyRIla-specific antibodies can be developed that do not recognize FcyRllb, which may be incorporated in Diabodies or Triabodies, to promote ADCC which is mediated by FcyRlla rather than by FcyRlllb) or which occur in different alleles (e.g. FcyRlla Arg/His-polymorphism at position 131, or FcyRllla Phe/Val-polymorphism at position 155). Another use of the novel peptides of the invention is a direct use as inhibitors of promoters of immunological functions. The peptides according to the invention may be used directly for immunotherapies. The above-described peptides of the invention can be produced by a novel method, wherein the method comprises: (a) - providing a protein of interest, (b) identifying a CDE on that protein, (c) producing a peptide comprising the sequence of the CDE, (d) structurally stabilizing the peptide so that the CDE is present in its native conformation. The peptide is structurally stabilized by circularisation, preferably by N- to C-terminal coupling, the formation of cysteine bridges, and/or bridging amino acid side chains forming a pseudopeptide. As stated above, the peptide is preferably generated using amino acids carrying glycosylation moieties which are present on the protein of interest. The method preferably comprises the additional step of conjugating the peptide to a carrier molecule which can be selected from any of the molecules mentioned above. Another aspect of the present invention is a peptide or polypeptide comprising a CDE, obtainable by the method of the invention. WO 2005/051999 PCT/EP2004/013450 -12- In order to significantly raise the fraction of specifically binding antibodies, the invention provides the following method for generating specific binding substances capable of discriminating between an antigen of interest and closely related antigens, wherein the method comprises immunising an animal with a peptide or polypeptide according to the present invention or/and with a correctly folded portion of the antigen of interest, in particular a peptide derived from an Fc receptor such as FcyRllb or FcyRlla, and isolating the resulting antibodies, and optionally using the antibodies to generate recombinant immunomodulatory substances. To produce antibodies that discriminate between an antigen A and an antigen B with high sequence identity to A, the differing amino acids are mapped to the structure of A or a respective structure model of A. Differing amino acids that are separated by several amino acids in the primary sequence may be in spatial proximity. In case that these differing amino acids are accessible from the solvent in the native structure these surface regions can be regarded as conformationally discriminating epitopes (CDE). Such epitopes can be artificially constructed by cyclic peptides or peptide analogues and are especially useful for the generation of antibodies that can discriminate between strongly related antigens. In a variation of this method transgenic animals are used for immunization that are engineered to express the close hornologue(s) and are later immunized with the target antigen. Animals that express the human FcyRllb are immunized with human FcyRlla or vice versa. In a particularly preferred aspect, the present invention provides an FcyRllb-binding antibody or fragment or derivative thereof, capable of specifically binding to FcyRllb or to FcyRIla in the natural environment of the Fc receptor. Such antibodies fragments or derivatives can discriminate between the closely related homologues of FcyRllb and FcyRlla in a natural environment, e.g in cell culture or in vivo. WO 2005/051999 PCT/EP2004/013450 -13- In a preferred embodiment, the FcyRllb- (or FcyRlla-) binding antibody or fragment or derivative thereof not only binds specifically to FcyRllb (or FcyRlla) but also prevents the natural binding partners of FcyRllb (or FcyRlla), i.e. IgG antibodies, from binding. In another preferred embodiment of the present invention, the specific anti-FcyRIIb (or anti- FcyRlla) antibodies are non-blocking and recognize an epitope distinct from the Fc-receptor/Fc-fragment interaction site(e.g. an epitope of the N-terminal domain around the amino acids 28-31). In contrast to blocking antibodies these antibodies have the advantage that binding of the receptor to immune complexes is not impaired. The result is that the activation of the receptors by immune complexes remains intact and additional receptors can be recruited to enhance the activation. It is thus possible to modulate the natural functions of these Fc receptors independent of IgG binding. For example, the antibody or fragment or derivative thereof may be chosen to be capable of crosslinking the Fc receptor. That way, the receptor can be activated. Preferably, the antibody or fragment or derivative thereof of the invention does not interfere with immune complex binding to FcyRllb or FcyRlla. On the other hand, the antibody or fragment or derivative thereof may be chosen so that it inhibits the physiological function of human FcyRlla or FcyRllb. The antibody or derivative or fragment of the invention preferably binds with higher affinity to FcyRiib than to FcyRlla. The antibody or fragment or derivative thereof binds FcyRllb with at least 5 fold, preferably at least 10 fold, preferably at least 100fold, more preferably at least 1,000fo!d, more preferably at ieast 10,000fold. more preferably at least 100,000fold, more WO 2005/051999 PCT/EP2004/013450 -14- preferably at least lO6oId, more preferably at least 107fold, more preferably at least lO6old, more preferably at least 109fold, more preferably 1010fold, more preferably 1011fold, more preferably 1012fold higher affinity than FcyRlla. Alternatively, the antibody or fragment or derivative binds FcyRlla with at least 5fold, preferably at least 10fold, preferably at least 100fold, more preferably at least 1,000fold, more preferably at least 10,000fold, more preferably at least 100,000fold, more preferably at least lO^fold, more preferably at least 107fold, more preferably at least lO^old, more preferably at least lO^old, more preferably 1010fold, more preferably 1011fold, more preferably 1012fold higher affinity than FcyRllb. 5, 10, 100, 1000 or even more than 1,000,000 fold tighter binding to the specific Fc-receptor is necessary to overcome the much higher expression level of FcyRlla on platelets over FcyRlfb. The antibody or fragment or derivative thereof can occur in a monomeric or multimeric state. The antibody or fragment or derivative thereof may be capable of binding Fc receptor molecules with or without cross-linking them on the cell surface. Preferably, the antibody or fragment or derivative thereof is multimeric in order to cross-link FcyRlla or FcyRHb. Alternatively, the antibody or fragment or derivative thereof is monomeric and able to block IgG binding to human FcyRllb, but preferably not able to cross-link FcyRIIb. The antibody or fragment or derivative thereof of the invention may also be modified in the Fc-fragment by the modification of the glycosylation and/or mutagenesis to enhance the binding towards subsets of the Fc-receptors. The antibody or fragment or derivative thereof of the invention is preferably able to bind to a CDE or/and peptide as described above, in particular those comprising one or more of the amino acids of human FcyRllb according to Figure 1 and SEQ ID NO: 2, selected from: Gin 12, Arg27, Thr29, His30, WO 2005/051999 PCT/EP2004/013450 -15- Va!104, Lys127, Ser132, Asn135, Tyr160, and Ala171, or the corresponding amino acids of FcyRlla according to SEQ ID MO: 1. More preferably, the substance binds to an epitope comprising'amino acids 27 to 30, and/or 127 to 135, and/or 160 to 171 of the amino acid sequence of FcyRllb (Figure 1, SEQ ID NO: 2) or the corresponding epitopes of FcyRlla. In a similar way human FcyRI I la-specific antibodies can be developed that do not recognize FcyRlllb, which may be incorporated in Diabodies or Triabodies, to promote ADCC which is mediated by FcyRllla rather than by FcyRlllb. The antibody or fragment or derivative thereof can be any natural, artificial or recombinantly produced substance carrying a region which can bind to the above-mentioned epitopes of FcyRllb. Preferably, this region contains the complementarity determining regions (CDRs) of the antibody which bind specifically to FcyRllb. More preferably, the CDRs comprise the sequences as depicted in Figures 5 and 6. The described CDRs maybe the basis for variations to further improve their specificity or designing new specific or pan-antibodies (or binding molecules) for other selected Fc-Receptors or receptor groups. Methods are known that include random or site directed mutagenesis, screening for related sequences and knowledge- or structure-based design. Preferably, the antibody or fragment or derivative thereof comprises one or both of the variable light and variable heavy regions according to SEQ ID Nos: 5 and 7, and/or the variable light and variable heavy regions according to SEQ ID Nos: 9 and 11. Most preferably, the antibody is CE5 or GB3. Monoclonal antibodies are preferred. Preferably, it is an antibody or fragment or derivative thereof having an IgG, IgE, IgM or IgA isotype. Preferably, the antibody is human or humanized, but may also be of other WO 2005/051999 PCT/EP2004/013450 -16- origin, such as animal origin, in particular of mouse or camel origin. The antibody may be in various forms, such as a single chain antibody, bi- or tri-functional or multi-functional antibody, Fab- of Fab2-fragment or as entire antibody in which the Fc-fragment has a modified affinity towards Fc receptors or complement. It may also be a Fab fragment, a F(ab)2 fragment, or a Fv fragment, or an scv fragment. The antibody or fragment or derivative thereof may also be a recombinantly produced polypeptide or polypeptide analogue which has a specific binding region comprising the sequence of the CDRs or a similar sequence related to more than 50 %, preferably more than 70 %, preferably more than 90 %, preferably more than 95 % to the provided sequences. These sequences may also be the starting point for the design of inhibitors of Fc-receptors. Therefore, also peptidomimetica are part of the invention that use or mimic sequence motives of the provided CDRs. In another preferred embodiment, the antibody or fragment or derivative thereof is an anticaline or lipocaline-variant or another antibody surrogate. The obtained antibody or fragment or derivative thereof can be coupled to an effector molecule such as an antigen of interest, antibodies, antibody fragments, marker molecules, cytotoxic substances, sterically bulky blocking substances and linker molecules and linker substances. Another aspect of the invention are nucleic acids, vectors and host cells containing nucleic acids encoding the peptides and/or the antibodies or fragments or derivatives thereof, of the present invention as described above. From the antibody or fragment or derivative thereof according to the invention, a nucleic acid sequence encoding this protein can be derived. Preferably, that sequence encodes the variable regions, preferably the CDRs binding to the above mentioned epitopes of FcyR!ib. Most preferably, WO 2005/051999 PCT/EP2004/013450 -17- the nucleic acid sequence encodes the CDRs according to one or more of the sequences according to Figures 5 and 6. Preferably, the nucleic acid encodes the sequence of monoclonal antibodies CE5 or GB3. The nucleic acid sequence may be inserted into a vector for the expression of the protein according to Figures 5 and 6, which vector is also an aspect of the present invention. The vector preferably comprises a promoter under the control of which the above nucleic acid sequences are placed. The vector can be prokaryotic or an eukaryotic expression vector, where the recombinant nucleic acid is either expressed alone or in fusion to other peptides or proteins or a vector suitable for DNA-vaccination. The invention also provides a host ceil transfected with the vector mentioned above. The host cell can be any cell, a prokaryotic cell or a eukaryotic cell. The present invention further provides a pharmaceutical composition useful for the treatment of diseases associated with Fc receptor mediated signaling, comprising an effective amount of the antibody or fragment or derivative thereof according to the invention, and pharmaceutically acceptable carrier substances. The present invention further provides a diagnostic kit for the detection of autoimmune diseases and/or cancer, comprising an antibody or fragment or derivative thereof according to the invention and/or the recombinant peptide or polypeptide according to the invention which comprises or represents one of the epitopes as described herein, and optionally marker reagents, carrier reagents and/or suitable receptacles. Immunization with unglycosylated correctly folded Fc-receptors, e.g. derived from E. co!i, and decorated'with the described epitopes surprisingly leads to antibodies that specifically recognize natural Fc-receptors expressed on blood cells and in cell culture (Figure 3 and Figure 4). WO 2005/051999 PCT/EP2004/013450 -18- Another aspect of the present invention is a method of producing antibodies characterized by the ability to specifically bind to FcyRllb, wherein the method comprises: (a) Providing the correctly folded FcyRllb molecule or portion thereof as an immunogen, comprising at least a part of the extracellular domain (conformational epitopes), their conjugation, or conjugation with other carrier molecules (e.g. KLH, BSA). (b) Immunizing a mammal with the immunogen of (a) and producing antibodies according to known methods, (c) Isolating the resulting antibodies or the cells producing these antibodies. The antibodies are preferably monoclonal antibodies. The CDRs may be grafted to other immunoglobulin classes (e.g. IgM, IgE, lgG1-lgG4) or other scaffolds (e.g. lipocaline-variants, camel antibodies), or mutated or derivatised molecules (e.g. engineered antibodies containing a modified Fc-fragment). The above described method may be used to produce vehicles for the immunization of animals and results in an anti-serum of increased specificity towards FcyRllb, which after fusion of isolated B-cells with myeloma cells results in hybridoma cells with an increased fraction producing antibodies specific for FcyRllb. The antibody or fragment or derivative thereof according to the present invention is useful for the production of a medicament for the treatment and/or diagnosis of conditions involving the immune system. Preferably, these conditions are autoimmune diseases or cancer. The diseases that can be treated with a medicament of the invention include, but are not limited to rheumatoid arthritis, psoriatic arthritis, WO 2005/051999 PCT/EP2004/013450 -19- ankyiosing spondylitis, Rieter's syndrome, psoriasis, multiple sclerosis, lupus erythematosus. Autoimmune diseases which can be diagnosed or treated using the substances of the present invention include, but are not limited ,to systemic lupus erythematosus, rheumatoid arthritis, Multiple Sclerosis, idiopathic thrombocytopenic purpura and host-versus-graft disease. Surprisingly, it has been found by the present inventors that it is possible to enhance certain immunological processes by using the FcyRllb-binding substances in vivo. In particular, it is possible to use those substances of the invention to specifically block the signaling of FcyRllb on cells and thereby increasing the immune response of the individual. This may be used to increase ADCC against tumor cells. In practice the FcyRllb-binding substance is given as adjuvant with a therapeutic antibody. The inhibitory signal transmitted by antigens (e.g. tumor cells) opsonized with the therapeutic antibody to activated macrophages or B-cells is blocked and the host immune system will be more effective in combating the targeted antigen. This can either be in a direct way by labeling tumor cells that express FcyRllb (e.g. B cell lymphoma) or by using this FcyRllb-binding substance as adjuvant in all approaches which use known therapeutic antibodies and therefore depend on the ADCC of the host. The known therapeutic antibodies include but are not limited to Herceptin®, Rituxan®, IC14, PANOREX™, IMC-225, VITAXIN™, Campath 1H/LDP-03, LYMPHOCIDE™ and ZEVLIN™. They can also include antibodies binding to the following cancer antigens: MAGE-1, MAGE-3, BAGE, GAGE-1, GAGE-2, N-acetylglucosaminyltransferase, pi5, beta-catenin, MUM-1, CDK-4, HER-2/neu, human papillomavirus E6, human papillomavirus E7 and MUC-1. In certain lymphomas B-cells or Mast-cells are transformed. The antibody or fragment or derivative thereof is able to cross link FcyRlib on the surface of WO 2005/051999 PCT/EP2004/013450 -20- these cells, which labels these cells for elimination but additionally an inhibitory and pro apoptotic signal is transmitted to these cells. This effect is an improvement of previous therapeutic antibody approaches, which completely depend on the ADCC of the host (e.g. Rituxan). The same antibody that crosslinks or blocks Fc-receptors may be used for the treatment of host-versus-graft disease or amyloid associated diseases. The same FcyRllb blocking and/or cross linking constructs maybe used to inhibit mast cells for the treatment of allergies. The antibody or fragment or derivative thereof maybe coupled to IgE (e.g. by transferring the CDRs shown in figure 5 or 6 to an IgE molecule). In this case the IgE is bound by the Mast-cell expressed FCERI and the FcyRllb specific CDRs cross link the ITAM of FCERI with the ITIM of the FcyRllb. Again an inhibitory arid/or apoptotic signal is transmitted to Mast-cells, which is useful in the therapy of allergies. The antibody or fragment or derivative thereof (e.g derivatives of the sequences depicted in figures 5 and 6) maybe used for the treatment of autoimmune diseases. Such substances inhibit B-cells, dendritc cells and activated ganulocytes (e.g Macrophages) which leads to a reduced production of immune stimulatory mediators and to a reduction in antibody production as well as antigen presentation (e.g. on Dendritic Cells and Macrophages leading to a decrease in T-ce!l recruitment). Taken together the feed back loop of antibody production and restimulation of the immune system is inhibited. Preferably the anti- FcyRlfb or FcyRlia does not interfere with Fc-fragment binding of the receptor. In this way the normal function of the Fc-receptor is in contrast to blocking antibodies maintained and enhances the activation of the cell bv the additional recruitment of further receptors. WO 2005/051999 PCT/EP2004/013450 -21- On the other hand specific anti- FcyRlla antibodies or fragments thereof maybe used in diabodies to direct an antigen towards this receptor or fragments of these antibodies maybe used to inhibit the uptake of immune complexes for example for the treatment of ITP. The CDRs can be used alone or in combination for the production of specific inhibitors of the FcyRlla/IgG interaction or the FcyR(lb/lgG interaction. For the generation of such inhibitors, derivatives or peptidomimetics as well as non-natural amino acids may be used. The inhibitors may in turn be used to generate crystal structures or for structure based design or as subject for evolutionary methods. A further use is the generation of modified sequences from that depicted in figures 5 or 6 by evolutionary methods (e.g. random or site directed mutagenesis or structure based design). In particular, the inhibitors of Fc receptors may be used to reduce or enhance the specificity of the above for the selected Fc-receptors. To this end, modifications can be carried out in the CDRs of the specific antibodies, in particular of GB3 and CE5, in order to enhance or lower their specificity to FcyRllb. The peptides and polypeptides and substances of the invention, in particular the antibody or fragment or derivative thereof are useful for the production of a medicament for the treatment and/or diagnosis of conditions involving the immune system, in particular autoimmune diseases, preferably those selected from Systemic Lupus Erythematosus, Rheumatoid Arthritis, Immune Thrombocytopenic Purpura or Multiple Sclerosis. Further uses of the peptides and antibodies or fragments or derivatives thereof of the invention are in the diagnosis and/or treatment of cancer and/or allergies. The mAbs CE5 or GB3 or derivatives or fragments thereof are particularly useful for the treatment of autoimmune diseases, Multiple sclerosis, Systemic Lupus WO 2005/051999 PCT/EP2004/013450 -22- Erythematosus, Idiopathic Thrombocytopenic Purpura, Rheumatoid Arthritis, and cancer, in particular lymphomas orleukemias. The rnAbs CE5 or GB3 or derivatives or fragments thereof can also be used for the treatment of cancer in combination with other therapeutics preferably biotherapeutics (e.g. antibodies). The antibody or derivatives or fragments thereof generated according to the present invention can be used for the treatment and/or diagnosis of cancer, preferably in combination with other therapeutics, preferably biotherapeutics (e.g. further antibodies). The antibody or fragment or derivative thereof is then preferably used as an adjuvant. Further uses of the antibody or fragment or derivative thereof of the invention include the use for the production of pharmaceutical and/or diagnostic compositions for the treatment of host-versus-graft disease, for the treatment of amyloid linked diseases or to increase the effect of vaccination or for the treatment of diseases associated activated dendritc cells and/or macrophages. It is also possible to use an antibody or fragment or derivative thereof which comprises specific anti- FcyRlla fragments in bi-specific antibodies to direct antigens towards transport by thrombocytes and/or uptake by the liver and spleen phagocytosis system. Preferably, the antibody or fragment or derivative thereof is a specific anti-FcyRIJa antibody or fragment thereof for the treatment of ITP. Description of Figures and Sequence Listing Figure 1: Sequence alignment of the extracellular domains of the human FcyRlIb and FcyRlla. Differing amino acids are boxed. WO 2005/051999 PCT/EP2004/013450 -23- Figure 2: Structure of FcyRllb in ribbon representation. The unique residues are shown in ball-and-stick and potential giycosylation sites are indicated as larger spheres. Arrows point to possible extractable substructures (epitopes 1 and 2) hat may be artificially generated for the improvement of immunization protocols towards specific FcyRllb-antisera and subsequently for the production of isoform specific monoclonal antibodies. Figure 3: Left diagram: Histogram of a FACS measurement of Raji cells (FcyRllb- positive and FcyRI la-negative) using the preimmune serum of the mouse (minus), the obtained antiserum after the immunization procedure (antiserum) and the pan-FcyRII-mAb AT10 (Greenman et al., 1991). Right diagram: Fluorescence label on U-937 cells (FcyRI la-positive and FcyRllb negative). The antiserum reacts only marginally with the cells indicating the presence of specific antibodies. Figure 4: FACS analysis of human blood incubated either with normal serum (negative control), antiserum of a mouse immunized with FcyRllb-CDE[126-137], mAb AT10 or the specific monoclonal antibody GB3 generated by using this invention, a): Dotblot analysis of the blood sample in terms of cell size (FSC-H) and granularity (SSC-H). The observed regions R1, R2 R3 contain lymphocytes (B and T cells), monocytes and granulocytes respectively, b) Fluorescence intensity of the cells found in region R1 representing lymphocytes. The pan-FcyRllb mAb AT10, the mAb GB3 and the antiserum stain the FcyRHb-positive B-cells while the FcyRII-negative T cells are not recognized, c) Fluorescence intensity of the cells found in region R2 representing monocytes/macro'pnages. In contrast to the positive controls mAb AT10 and the antiserum the mAb GB3 does not recognize the FcyRlla-positive monocytes. d) Fluorescence intensity of the cells found in region R3 representing granulocytes. In contrast to the positive controls mAb AT10 and WO 2005/051999 PCT/EP2004/013450 -24- the antiserum the mAb GB3 does not recognize the FcyRI la-positive granulocytes. Figure 5: The variable regions of the cloned antibody GB3. The boxed regions represent the CDRs while the underlined termini may vary due to cloning artifacts introduced by the primer, a) Variable region of the light chain; b) Variable region of the heavy chain. Figure 6: The variable regions of the cloned antibody CE5. The boxed regions represent the CDRs while the underlined termini may vary due to cloning artifacts introduced by the primer, a) Variable region of the light chain; b) Variable region of the heavy chain. Figure 7: The glycopeptide CDE[126-137] used for immunization and generation of FcyRIIb-specific antibodies. Figure 8: Immunisation of SJL mice with a specific anti-mouse FcyRII antibody. SJLj were immunized with 200ug MOG an day 0. Treatment with antiFcyRII antibody (dosis of 50ug/week) started at day 5. The clinical score was monitored daily and is given as the average of the 8 mice per group. SEQ ID NO: 1 amino acid sequence of FcyRlla (as in Fig. 1) SEQ ID NO: 2 amino acid sequence of FcyRllb (as in Fig. 1) SEQ ID NO: 3 sequence of the glycopeptide CDE [126-137] SEQ ID NO: 4 nucleic acid sequence of the variable light region of WO 2005/051999 PCT/EP2004/013450 -25-mAbGB3 SEQ ID NO: 5 corresponding amino acid sequence of the variable light region of mAb GB3 SEQ ID NO: 6 nucleic acid sequence of the variable heavy region of mAb GB3 SEQ ID NO: 7 corresponding amino acid sequence of the variable heavy region of mAb GB3 SEQ ID NO: 8 nucleic acid sequence of the variable light region of mAb CE5 SEQ ID NO: 9 corresponding amino acid sequence of the variable light region of mAb CE5 SEQ ID NO: 10 nucleic acid sequence of the variable heavy region of mAb CE5 SEQ INO: 11 corresponding amino acid sequence of the variable heavy region of mAb CE5 Examples Example 1 Synthesis of the Cyclo-[N-b-(2-acetylamino-deoxy-2-p-glucopyranosyl)- Asn13B, Gly141M129-141)-FcyRHb2, CDE[126-137] Standard amino acid derivatives were from Alexis (Laufelfingen, Switzerland), Fluorenyimethoxycarbonyl-derivative (Fmoc) of Asn(A/-p-3,4,6-tri-0-acetyl-2-acetylamino-deoxy-2-b-glucopyranosyl)-OH from Merck- WO 2005/051999 PCT/EP2004/013450 -26- Novabiochem (Darmstadt, Germany), and the preloaded chlorotrityl resin from Pepchem (Tubingen, Germany). Reagents and solvents were of the highest quality commercially available and were used without further purification. Analytical reversed-phase HPLC was performed on Waters equipment (Eschborn, Germany) with a Symmetry C18 column (5 urn, 3.9x150 mm, Waters) by linear gradient elution: (1) 0-100 % A in 15 min, or (2) 0-3O % A in 20 min, up to 50 % A in 5 min and to 100 % A in further 5 min, (flow rate of 1.5 m!/min and UV detection at 210 nm). The binary elution system was (A) acetonitrile/2 % H3PO4 (90:10) and (B) acetonitrile/2 % H3PO4 (5:95). Preparative reversed-phase HPLC was carried out on Abimed equipment (Langenfeld, Germany) using Nucleosil Ci8 PPN (5 u.m, 100 A, 10x250 mm, Macherey-Nagel, Duren, Germany) and a gradient of 0.08 % trifluoroacetic acid (TFA) in acetonitrile (A) and 0.1 % TFA in water (B) at a flow rate of 10 ml/min: 2 % A for 7 min, up to 40 % A in 50 min and to 70 % A in further 5 min. ESl-MS spectra were recorded on a Perkin-Elmer SCIEX API 165 triple quadrupole spectrometer. LC-MS was carried out with a Nucleosil C18 column (5u.m, 100 A, 1x250 mm, Macherey-Nagel) using linear gradients of 0.1 % TFA in water and 0.08 % TFA in acetonitrile (flow rate: 30 pil/min; detection at 210 nm). a) Solid-Phase Peptide Synthesis. The linear peptide precursor was synthesized manually on Fmoc-Gly-chiorotrityl resin (232 mg, 0.13 mmol) following standard procedures of Fmoc/fe/t-butyl (tBu) chemistry. The Frnoc group was cleaved in each step with two successive treatments (3 and 20 min) with 20 % piperidine in N-methyl pyrrolidone (NMP). For Fmoc-Ser(tBu)-OH and Fmoc-Phe-OH double couplings (2x1 h) with Fmoc-amino acid/2-(1/-/-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafiuoro-phosphate (HBTU)/A/-hydroxybenzotriazole (HOBt)/A/,A/-diisopropy!ethylamine (DJEA) (4:4:4:8 eq) in NMP were applied, whereas the glycosylated Asn derivative was introduced by single coupling using Fmoc-aminoacid/(1H-benzotriazol-1-y!oxy)-tripyrroIidinophosphonium WO 2005/051999 PCT/EP2004/013450 -27- hexafiuorophosphate (PyBOP)/HOBt/DIEA (2:2:2:5 eq) in NMP. The reaction was complete after 5 h, as confirmed by the Kaiser test. A capping step with acetic anhydride/DlEA (1:1, 3 eq) for 10 min was performed prior to chain elongation. For acylation with the remaining amino acid derivatives (Arg was introduced as Arg^^AS.Z-pentamethyl-dihydrobenzofurane-S-sulfony! [Pbf] derivative) again double couplings (2x1.5 h) were used with Fmoc-amino acid/HBTU/HOBt/DlEA (6:6:6:12 eq) in NMP. b) Cleavage of the Side-Chain-Protected Peptide. The side-chain-protected linear peptide was cleaved from the resin by treating the peptide-resin with 5 ml of 1 % TFA in dichloromethane (DCM) for 3 min. The filtrate was analyzed by thin layer chromatography (TLC) (CH3CI/MeOH/H2O, 8:3:1) prior to addition of 1 ml of 10 % pyridine in methanol. The TFA treatment was repeated until the TLC control on the filtrate was negative (overall four treatments). Finally, the resin was washed with DCM and trifluoroethanol to improve the peptide recovery. The peptide-containing filtrates and the final washes were combined and concentrated to a small volume. The residue was diluted with MeOH, and the product was precipitated with ice-cold water. The crude product was collected by filtration (270 mg, 80 % yield) and characterized by analytical HPLC (gradient 1) and ESI-MS. A major peak (t« 9.5 min; ESI-MS: m/z = 2520 [M+H]+; Mr = 2519.0 calcd for Ci2oHi88N2o036S) and a minor peak (tR 9.3 min; ESI-MS: m/z = 2478 [M-42+H]+) at the ratio of 75:20 were found to correspond to the expected product and to a side product, respectively. The mass difference was attributed to the loss of one acetyl protecting group from Asn(Ac3AcNH-p-Glc). c) Cycfization. Backbone cyclization was accomplished at a peptide concentration of 0.9 mM in N,N-dimethylformamide (DMF), in the presence of PyBOP/HOBt/DlEA (1.5:1.5:3.5 eq). The base was added in portions over 1 h. The conversion of WO 2005/051999 PCT/EP2004/013450 -28- the linear peptide to the cyclic form was monitored by analytical HPLC, and was completed after 2.5 h. The reaction mixture was taken to dryness, and the residue was triturated and washed with ice-cold diethyl ether to remove traces of DMF prior to the TFA cleavage. d) Cleavage of the Side-Chain Protecting Groups. The acid-labile side-chain protecting groups were removed by dissolving the cyclic peptide in 10 ml the ice-cold TFA/triisopropylsilane (TISH)/H2O (90:5:5). After 2 h shaking, the TFA was removed under reduced pressure, the oily residue was diluted with a small amount of MeOH and the crude product precipitated with ice-cold diethyl ether. The precipitate was collected by centrifugation, washed several times with ice-cold ether and, finally, lyophilized from water. The crude glycopeptide which in addition to the triacetylated form, according to LC-MS was contaminated by the di- and mono-acetyl derivatives, was suspended in MeOH and treated in portions with NaOMe over 30 min until an apparent pH of > 10 was reached. The reaction was monitored by HPLC, and after 3.5 h it was quenched by addition of glacial acetic acid until pH 95 % (tR 7.37 min with gradient 2); ESI-MS: m/z = 1642.8 [M+H]+; M = 1641.8 Da calculated for C7IHIOBN2O025- Coupling of the'CDE[126-137] to FcyRHb yielding FcyRHb-CDE[126-137] 100M! human soluble FcyRllb (10.6mg/m!) were added to 1490u! 50mM borate pH10 and 410ul of the glycopeptide CDE[126-137] (2mg/ml) and stirred gently at room temperature. 100ul of a 0.3% glutaraidehyde solution were slowly added and the whole mixture stirred for another two hours at RT WO 2005/051999 PCT/EP2004/013450 -29- before 100ul 1M glycine was added. The resulting FcyR!lb-CDE[126-137] was stirred for another 30min and then dialyzed against PBS and concentrated. Example 2 Immunization with FcyRHb-CDE[126-137] A female six weeks old C57B1/6 mouse was immunized intra peritonea I ly every two weeks with an emulsion of 50ug FcyRllb-CDE[126-137] in 100[il Complete Freunds Adjuvant (CFA, Sigma/Deisenhofen, Germany) for three times. Three weeks after the last immunization the mouse was boosted with 50ug of the FcyRllb-CDE[126-137]: Three days later the spleen was removed from the animal and the fusion of the extracted cells with myeloma cells was performed according to Bazin, and Lemieux, 1989. Example 3 Screening of the hybridoma for FcYRHb-CDE[126-137]-specificity Clones that were able to grow in the presence of hypoxanthine, aminopterin, and thymidine were isolated and their supernatant tested in ELISA assays where FcyRllb-CDE[126-137] was precoated on microtitre plate with 120 ng sFcyRlla/b per well (in 100 pi PBS, 20 °C, 12h). The plate was washed and incubated with PBS/T (PBS/0.2 % Tween20, 30 min). 100ul of the respective hybridoma were added to the well (100 ul, 90 min). The plate was washed three times with blocking buffer before 100ul of a peroxidase labeled goat-anti mouse IgG+IgM antibody (Dianova, Hamburg/Germany) diluted in PBS/T was added. After incubating for 90 min and subsequent washing with PBS/T, 100 ul of substrate buffer (0.2 M citrate/phosphate buffer pH 5.2, 4 mg/ml o-phenylenediamine, 6.024 % (v/v) hydrogenperoxide) were applied to the wells. The reaction was stopped by adding 50 ul 8 N sulfuric acid and the absorbance at 490 nrn was measured in an ELISA reader. WO 2005/051999 PCT/EP2004/013450 -30- Clones that were positive in this assay were tested by flow cytometry (FACS) using 105 Raji cells per sample (ATCC CCL-86) which strongly express human FcyRllb. After incubation with 100ul hybridoma supernatant for 30 min on ice the cells were washed with 1 ml RPMI/10% FCS and precipitated by centrifugation (400 x g, 4 °C, 5 min). 100 ul FITC labeled goat anti human antibody (Dianova, Hamburg/Germany) were added. After incubation for 30 min on ice the cells were washed (RPMI/10 % FCS) and subjected to flow cytometry (FACSort, Becton Dickinson, Heidelberg/Germany). The median value of the fluorescence for 5,000 counted cells was determined for each sample. Hybridoma supematants that were positive in .this assay were subjected in a similar assay using U-937 cells (ATCC CRL-1593.2) which strongly express FcyRlla to determine FcYRIIb-specificity of the hybridoma. As positive control for both cell lines the pan-FcyRII-mAb AT10 (Greenman et al., 1991) was used. Example 4 Immunisation of SJL mice with a specific anti-mouse FcyRII antibody SJL-Mice were immunized with 200ug MOG to induce Experimental Autoimmune Encephalomyelitis (EAE) an established animal model of Multiple Sclerosis. Prophylactic as well as therapeutic (data not shown) treatment of 8 mice per group with a specific anti-mouse FcyR!! antibody (50 ug/ week) significantly reduces the symptoms (clinical score )of the disease (O=healthy,1=light paralysis,2=medium paralysis, 3=strong paralysis, 4=complete paralysis, 5=death). The results are shown in Figure 8. WO 2005/051999 PCT/EP2004/013450 -31 -References Amigorena, S., Bonnerot, C, Drake, J.R., Choquet, D., Hunziker, W., Guillet, J.G., Webster, P., Sautes, C, Mellman, I., Fridman, W.H. (1992), Cytoplasmic domain heterogeneity and functions of IgG Fc receptors in B lymphocytes, Science 256, 1808-1812. Bazin, R. and Lemieux, R. (1989), Increased proportion of B cell hybridomas secreting monoclonal antibodies of desired specificity in cultures containing macrophage-derived hybridoma growth factor (IL-6). J. Immunol. Methods 116,245-249. Ceuppens, J.L, Baroja, ML, van Vaeck, F., Anderson, C.L. (1988), Defect in the membrane expression of high affinity 72kD Fc© receptors on phagocytic cells in four healthy subjects, J. Clin. Invest. 82, 571-578. Clynes, R.A., Towers, T.L., Presta, L.G., Ravetch, J.V. (2000), Inhibitory Re receptors modulate in vivo cytotoxicity against tumour targets. Nature Medicine 6, No. 4, 443-446. Engelhardt, W., Geerds, C, Frey, J. (1990), Distribution, inducibility and biological function of the cloned and expressed human © Fc receptor il, Eur. J. Immunol. 20, 1367-1377. Fridman, W.H., Bonnerot, C, Daeron, M., Amigorena, S., Teillaud, J.-L, Sautes, C. (1992), Structural bases of FcyR functions, Immunol. Rev. 125, 49-76. Fridman, W.H., Teillaud, J.-L, Bouchard, C, Teillaud, C, Astier, A., Tartour, E., Galon, J., Mathiot, C, Sautes, C. (1993), Soluble Fc© receptors, J. Leukocyte Biol. 54, 504-512. Greenman, J., Tutt, A.L., George, A.J., Pulford, K.A., Stevenson, G.T., Giennie, MJ. (1991), Characterization of a new monoclonal anti-Fc gamma Ril antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol. Immunol. 28, 1243-1254. Homsy, J., Meyer, M., Tateno, M., Clarkson, S., Levy, J.A. (1989), The Fc and not CD4 receptor mediates antibody enhancement of HIV infection in human cells, Science 244, 1357-1360. WO 2005/051999 PCT7EP2004/013450 -32- Isakov N. (1997), ITIMs and ITAMs. The Yin and Yang of antigen and Fc receptor-linked signaling machinery. Immunol Res. 16, 85-100. Littaua, R., Kurane, I. and Ennis, FA (1990), Human IgG FcyR II mediates antibody-dependent enhancement of dengue virus infection, J. Immunol. 144,3183-3186. Metzger, H. (1992A), Transmembrane signaling: The joy of aggregation, J. Immunol. 149,1477-1487. Metzger, H. (1992B), The receptor with high affinity for IgE, Immunol. Rev. 125, 37-48. Poo, H., Kraus, J.C., Mayo-Bond, L, Todd, R.F., Petty, H.R. (1995), Interaction of FcyRIIIB with complement receptor type 3 in fibroblast transfectants: evidence from lateral diffusion and resonance energy transfer studies, J. Mol. Biol. 247, 597-603. Ravanel, K., Castelle, C, Defrance, T., Wild; T.F., Charron, D., Lotteau, V., Rabourdincombe, C. (1997), Measles virus nucleocapsid protein binds to FcyRII and inhibits human B cell antibody production. J. Exp. Med. 186, 269-278. Ravetch, J. V. and Bolland, S. (2001), IgG Fc Receptors. Annu. Rev. Immunol. 19, 275-290. Sondermann, P., Jacob, U., Kutscher, C, Frey J. (1999A), Characterization and crystallization of soluble human Fey receptor II (CD32) isoforms produced in insect cells. Biochemistry. 38, 8469-8477. Sondermann, P. and Jacob, U. (1999B), Human Fey receptor lib expressed in E. coli reveals IgG binding capability. Biol Chern. 380, 717-721. Sondermann P, Huber R, Oosthuizen V, Jacob U. (2000), The 3.2A crystal structure of the human lgG1 Fc fragment-FcyRIII complex. Nature 406, 267- 273. Sondermann P, Kaiser J, Jacob U. (2001), Molecular basis for immune complex recognition: a comparison of Fc-receptor structures. J Mol Bio!. 309, 737-749. WO 2005/051999 PCT/EP2004/013450 -33- van de Winkel, J.G.J. and Capel, P.J.A. (1993), Human IgG Fc receptor heterogeneity: Molecular aspects and clinical implications, Immunol. Today 14,215-221. Yang, Z., Delgado, R., Xu, L, Todd, R.F., Nabel, E.G., Sanchez, A., Nabel, G.J. (1998), Distinct cellular interactions of secreted and transmembrane Ebola virus glycoproteins, Science 279, 983-984. Zhou, M.-J., Todd, RJF., van de Winkel, J.G.J., Petty, H.R. (1993), Cocapping of the leukoadhesin molecules complement receptor type 3 and lymphocyte function-associated antigen-1 with FcyRIII on human neutrophils. Possible role of lectin-like interactions, J. Immunol. 150, 3030-3041. WO 2005/051999 PCT/EP2004/013450 -34- WE CLAIM: 1. An antibody or fragment thereof, which specifically binds to either human FcyRIIb or FcyRIIa in the natural environment of the Fc receptor and which is capable of binding to a conformationally discriminating epitope (CDE) of FcyRIIb or FcyRIIa. 2. The antibody or fragment thereof as claimed in claim 1, which binds with higher affinity to FcyRIIb than to FcyRIIa, preferably with at least 10fold, more preferably at least 100fold, more preferably at least l,000fold, more preferably at least 10,000fold, more preferably at least 100,000fold, more preferably at least l,000,000fold higher affinity to FcyRIIb than to FcyRIIa. 3. The antibody or fragment thereof as claimed in claim 1, which binds with higher affinity to FcyRIIa than to FcyRIIb, preferably with at least lOfold, more preferably at least 100fold, more preferably at least l,000fold, more preferably at least 10,000fold, more preferably at least 100,000fold, more preferably at least l,000,000fold higher affinity to FcyRIIa than to FcyRIIb. 4. The antibody or fragment thereof as claimed in claim 1,2 or 3, which is able to specifically block IgG binding to human FcyRIIb or FcyRIIa. 5. The antibody or fragment thereof as claimed in claim 1,2,3 or 4, which is capable of inhibiting the physiological function of human FcyRIIb or FcyRIIa. 6. The antibody or fragment thereof as claimed in claim 1 to 3, which does not interfere with immune complex binding to FcyRIIb or FcyRIIa. 7. The antibody or fragment thereof as claimed in claim 1,2,3 or 6, which is capable of activating the physiological function of human FcyRIIb or FcyRIIa. 8. The antibody or fragment thereof as claimed in claim 1,2,3,6 or claim 7, which is able to specifically cross-link human FcyRIIb or FcyRIIa. 9. The antibody or fragment thereof as claimed in any one of claims 1 to 8, which occurs in a monomelic or multimeric state. 10. The antibody or fragment thereof as claimed in anyone of claims 1 to 9, which is capable of binding to an epitope of human FcyRIIb or FcyRlla comprising at least one of amino acids 12,27,29, 30, 104,127 132, 135, 160 and 171 of the amino acid sequence of FcyRIIb or FcyRlla according to SEQ ID NO: 1 or SEQ ID NO: 2. 11. The antibody or fragment thereof as claimed in claim 10, which is a capable of binding to an epitope of FcyRIIb or FcyRlla comprising amino acids 27 to 30, and/or 127 to 135, and/or 160 to 171 of the amino acid sequence of FcyRIIb or FcyRlla according to SEQ ID NO: 1 or SEQ ID NO: 2. 12. The antibody or fragment thereof as claimed in any one of claims 1 to 11, which is a polypeptide carrying a complementarity determining region (CDR) which is specific for FcyRIIb. 13.The antibody or fragment thereof as claimed in claim 12, which is a polypeptide carrying one or more of the CDR- sequences according to SEQ ID Nos : 5,7, 9 and 11. 14. The antibody or fragment thereof as claimed in any one of claims 1 to 13, which is of the class IgG, IgE, IgM or IgA. 15. The antibody or fragment thereof as claimed in any one of claims 1 to 14, wherein is selected from single chain antibodies, bi-functional antibodies and tri-functional antibodies, Fab fragments, F(ab)2 fragments, Fv fragments and scv-fragments. 16. An antibody or part thereof, as claimed in any one of claims 1 to 15, characterised in comprising the variable light and/or heavy regions of antibody GB3 according to SEQ ID NO: 5 and 7, or a portion thereof having specificity; or the variable light and/or heavy regions of antibody CE5 according to SEQ ID NO: 9 and 11 or a portion thereof having specificity. 17. An antibody or part thereof as claimed in any one of claims 1 to 16, which can specifically recognize alleles of the FcyRIIa Arg/His polymorphism at position 131 or the FcyRIIa Val/Phe polymorphism at position 155. 18.A nucleic acid sequence encoding the antibody or fragment thereof as claimed in any one of claims 1 to 17. 19. The nucleic acid as claimed in claim 18 encoding monoclonal antibodies CE5 or GB3 according to SEQ ID NOs: 4,6, 8 and 10 or a portion thereof. 20.A nucleic acid vector comprising the nucleic acid sequence as claimed in claim 18 or 19. 21. An artificial peptide or polypeptide comprising a conformationally discriminating epitope (CDE) of FcyRIIb and FcyRIIa, the CDE comprising at least residue which is unique to either FcyRIIb or FcyRIIa, wherein the CDE comprises amino acids 27 to 30, and/or amino acids 127 to 135, and/or 160 to 171 of FcyRIIb according to SEQ ID NO:2 or the corresponding amino acids of FcyRIIa according to SEQ ID NO: 1, or the amino acid sequence according to SEQ ID NO: 3, and wherein said peptide or polypeptide is structurally stabilized by circularization. 22.The peptide or polypeptide as claimed in claim 21, wherein it comprises artificial or/and glycosylated amino acids. 23. The peptide or polypeptide as claimed in claim 21 or 22, wherein it is conjugated to a carrier molecule. 24. The peptide or polypeptide as claimed in any one of claims 21 to 23, wherein it is conjugated to FcyRIIb or FcyRIIa. 25. A pharmaceutical and/or diagnostic composition useful for the treatment and/or diagnosis of diseases associated with Fc receptor mediated signaling, in particular rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Rieter's syndrome, psoriasis or lupus erythematosus, comprising an effective amount of the antibody, fragment or derivative thereof as claimed in any one of claims 1 to 17, and pharmaceutically acceptable carrier substances. 26. A diagnostic kit for the detection of autoimmune diseases and/or cancer, comprising the antibody, fragment or derivative thereof as claimed in any one of claims 1 to 17. 27. Antibody or fragment thereof as claimed in any one of claims 1 to 17, wherein it is modified in the Fc-fragment by the modification of the glycosylation and/or mutagenesis to enhance the binding towards subsets of the Fc-receptors. The invention relates to novel immunogens carrying conformationally discriminating epitopes (CDEs) and to immunization methods for producing antibodies that specifically recognize proteins with very closely related homologues In particular, the invention relates to antibodies which are specific for either FcyRllb or FcyRlla

Documents:

01675-kolnp-2006 correspondence other-1.1.pdf

01675-kolnp-2006 form-26.pdf

01675-kolnp-2006 international search authority report-1.1.pdf

01675-kolnp-2006 other document.pdf

01675-kolnp-2006 pct other.pdf

01675-kolnp-2006-abstract.pdf

01675-kolnp-2006-claims.pdf

01675-kolnp-2006-correspondence other.pdf

01675-kolnp-2006-correspondence-1.2.pdf

01675-kolnp-2006-description (complete).pdf

01675-kolnp-2006-drawings.pdf

01675-kolnp-2006-form-1.pdf

01675-kolnp-2006-form-18.pdf

01675-kolnp-2006-form-2.pdf

01675-kolnp-2006-form-3.pdf

01675-kolnp-2006-form-5.pdf

01675-kolnp-2006-international publication.pdf

01675-kolnp-2006-international search authority report.pdf

01675-kolnp-2006-pct form.pdf

01675-kolnp-2006-priority document.pdf

1675-KOLNP-2006-(30-01-2012)-CORRESPONDENCE.pdf

1675-KOLNP-2006-(30-01-2012)-PA.pdf

1675-KOLNP-2006-ABSTRACT 1.1.pdf

1675-KOLNP-2006-AMANDED CLAIMS.pdf

1675-KOLNP-2006-CANCELLED PAGE.pdf

1675-KOLNP-2006-CLAIMS 1.1.pdf

1675-KOLNP-2006-DESCRIPTION COMPLETE 1.1.pdf

1675-KOLNP-2006-DRAWINGS 1.1.pdf

1675-KOLNP-2006-FORM 1 1.1.pdf

1675-KOLNP-2006-FORM 2 1.1.pdf

1675-KOLNP-2006-FORM 27.pdf

1675-KOLNP-2006-FORM 3 1.1.pdf

1675-KOLNP-2006-FORM 5 1.1.pdf

1675-KOLNP-2006-FORM-27-1.1.pdf

1675-KOLNP-2006-FORM-27-1.pdf

1675-KOLNP-2006-FORM-27.pdf

1675-KOLNP-2006-REPLY F.E.R 1.1.pdf

1675-KOLNP-2006-REPLY F.E.R.pdf

1675-KOLNP-2006-SEQUENCE LISTING.pdf