Title of Invention

PROCESS FOR IDENTIFICATION OF COMPOUNDS IN A COMPOSITION OF RADIX SALVIAE MILTIORRHIZAE AND RADIX NOTOGINSENG

Abstract The present invention discloses a preparation for cardio-cerebral blood vessel diseases, it is prepared through extracting danshen and Notoginseng by lye, precipitating with alcohol, concentrating, and adding other medicine and excipients. Then using the HAPLY-MS and HAPLY fingerprint Atlas to characterize its Physicochemical properties completely. Using the fingerprint Atlas analysis method of the present invention, the structure and comparative content of biology active component can be known. Characterization of the physical chemical properties of danshen and Notoginseng of preparation with this way is better than other methods of the prior art. Figure 1 is the representative figure.
Full Text TECHNICAL FIELD
The invention relates to a Preparation for the treatment of Cardiovascular and cerebrovascular diseases and Process for preparing the same.
BACKGROUNID ART
Cardiovascular and cerebrovascular diseases are common ones which do great harm to health of human beings. Recently, such diseases have an increasing occurrence due to the changes of works, livings, diet patterns, environments and the like with social development. The traditional Chinese medicine (TCM), in spite of its lower activity toward a single target relative to the Western medicine, is characterized by its multiple routes and targets, dynamic and holistic treatment, and low side effects, which are far beyond the effects of the Western medicine. The TCM preparation with definite therapeutic effect will have an overall therapeutic effect superior to that of the Western medicine. There have been now a plurality of TCM preparations for the treatment of cardiovascular and cerebrovascular diseases, such as compound Danshen tablets and its TCM preparations, Guanxin Danshen drop pills, and Xinkeshu tablets etc. These TCM preparations, all of which contain Radix Salviae Miltiorrhizae (also known as danshen) and Radix Notoginseng, have different therapeutic effects for their different formulations, proportions of ingredients, extraction and purification processes, or dosage forms. In addition, these TCM preparations can hardly be controlled in quality, since no effective quality detection method is available at present for completely characterizing the physical and chemical properties of these medicines, and instead, only one or two compounds, such as Danshensu or Tanshinone II A, are used to represent the complex biologically active ingredients in these medicines. Therefore, it is necessary to improve the process for extracting and purifying such TCM preparations and also the method for controlling their qualities.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide a more effective TCM preparation for the treatment of cardiovascular and cerebrovascular diseases. Also provided herein is a detection method for relatively complete and exact characterization of the physical and chemical properties thereof.
It is another object of the present invention to provide a process for preparing the above TCM preparation.
The objects of the present invention are achieved through the following embodiments.
The TCM preparation according to the present invention can be prepared through a process comprising the following steps of:
mixing Radix Salviae Miltiorrhizae and Radix Notoginseng with sodium hydroxide, sodium bicarbonate, sodium carbonate, potassium hydroxide, potassium bicarbonate, potassium carbonate or a mixture thereof in an amount of 0.5%-4.0% based on the total weight of said medicinal materials to obtain a mixture;
boiling the mixture out in 3-6 folds of water for 2-4 times;
subjecting the mixture to filtration and concentrating the combined filtrates;
adding an ethanol with a high concentration (above 70%) in an amount sufficient to obtain a 65-70% content of the ethanol;
allowing the mixture to stand and separating the supernatant;
recovering the ethanol from the supernatant and concentrating the residue until it has a relative density of 1.20-1.50 (55-60°C), which is an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng;
mixing the above extract with Bomeol (or an oil of Lignum Dalbergiae Odoriferae); and
adding one or more pharmacological excipients, such as starch, dextrin, lactose, microcrystalline cellulose, hydroxypropyl methyl cellulose, polyethylene glycol, magnesium stearate, micro silicon gel, xylitol, lactitol, glucose, glycine, mannitol, methyl starch sodium, cross-linked sodium carboxyl methyl cellulose, cross-linked polyvinylpyrrolidone etc., to formulate the mixture into various dosage forms, such as injection, tablet, sustained-release tablet, drop pill, granule, injection powder, capsule, microgranule, oral disintegrant.
Preferably, the above TCM preparation is prepared through a process comprising the following steps of:
weighing Radix Salviae Miltiorrhizae and Radix Notoginseng;
adding sodium bicarbonate in an amount of 1.4%-1.9% based on the total weight of said medicinal materials to obtain a mixture;
boiling the mixture out in 4-5 folds of water for 2-3 hours, and then in 3-4 folds of water for another 1-2 hours;
subjecting the mixture to filtration and concentrating the combined filtrates until a specific gravity of 1.16-1.20 (80±5°C) is achieved;
adding an ethanol with a high concentration (above 70%) in an amount sufficient to obtain a 65-70% content (20 °C) of the ethanol;
allowing the mixture to stand for 8-12 hours and separating the supernatant;
recovering the ethanol from the supernatant and concentrating the residue until it has a relative density of 1.32-1.40 (55-60°C), which is an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng;
mixing the above extract with Borneol (or an oil of Lignum Dalbergiae Odoriferae); and
adding one or more pharmacological excipients selected from the group consisting of starch, dextrin, lactose, microcrystalline cellulose, hydroxypropyl methyl cellulose, polyethylene glycol, magnesium stearate, micro silicon gel, xylitol, lactitol, glucose, glycine, mannitol, methyl starch sodium, cross-linked sodium carboxyl methyl cellulose, cross-linked polyvinylpyrrolidone etc. to formulate the mixture into tablet, drop pill, injection powder, capsule, granule, microgranule, or oral disintegrant.
The Bomeol used herein can be a naturally occurring or synthesized one. The oil of Lignum Dalbergiae Odoriferae used herein is obtained through distillation of Lignum Dalbergiae Odoriferae.
The above TCM preparation is preferably hi the dosage form of drop pill.
The TCM preparation according to the present invention is characterized using the following physical and chemical parameters:
in the HPLC spectrum, there are 8 peaks which have a ratio of single peak area to total peak area greater than 2%; the average retention time of these 8 peaks is 6.04, 9.90, 16.89, 17.84, 20.31,23.74,27.73 and 31.02 respectively, and the RSD% of the retention time is 0.31, 0.25, 0.61, 0.70, 0.96,0.76,0.50 and 1.18 respectively; their average peak area is 1627.92,2575.54. 366.89, 381.40, 186.08, 555.35, 281.91 and 1852.33 respectively, and the RSD% of the peak area is 5.91, 13.53, 10.92, 13.81, 12.04, 10.48, 18.08 and 14.84 respectively; and the ratio of single peak area to total peak area accounts for 19.6%-22.0%, 28.5%-37.4%, 4.2%-5.2%, 4.2%-5.5%, 2.1%-2.7%, 6.4%-7.8%, 3.0%-4.3% and 20.2%-27.2% respectively.
The above physical and chemical parameters were obtained under the following detection conditions:
(1) High performance liquid chromatography
Octadecylsilyl-silica gel was used as a filler for the chromatography column, with flow rate of 1.000 ml/min and detection wavelength of 280 nm. The Elution was carried out under the following conditions: mobile phase A being a 0.02% aqueous phosphoric acid solution, mobile phase B being a 80% acetonitrile-0.02% aqueous phosphoric acid solution, mobile phase A being changed from 90% to 78% homogeneously and mobile phase B being changed from 10% to 22% homogeneously during 0 to 8 min; mobile phase A from 78% to 74% and mobile phase B from 22% to 26% during 8 to 15 min; and mobile phase A from 74% to 48% and mobile phase B from 26% to 52% during 15 to 55 min.
(2) Preparation and determination of sample solution
10 pills of the TCM preparation according to the present invention are weighed accurately, and then, added into a 10 ml measuring bottle. Distilled water was added in an amount sufficient to dissolve the pills through shaking with ultrasound for 15 minutes. And more distilled water was then added to achieve a volume of 10 ml. The resultant solution was subjected to centrifugation or filtration to obtain a sample solution. An accurate 10 ul of the sample solution was injected into a HPLC apparatus, and then determined by way of HPLC chromatography to obtain a HPLC spectrum.
With the aid of an analysis method, such as a comparison with a standard sample and Mass Spectra, the above 8 peaks with an average retention time of 6.04, 9.90, 16.89, 17.84, 20.31, 23.74, 27.73 and 31.02 were identified to correspond with Danshensu, Protocatechualdehyde, Isolithospennic acid A, Isolithospemiic acid B, Salvianolic acid D, Rosmarinic acid, Salvianolic acid B and Salvianolic acid A, respectively (see figure 1).
Using a particular HPLC-MS method, the TCM preparation of the present invention was determined to comprise Danshensu, Protocatechualdehyde, Isolithospermic acid A, Isolithospennic acid B, Salvianolic acid D, Salvianolic acid E, Rosmarinic acid, Salvianolic acid B, Salvianolic acid G, Salvianolic acid A, Tanshinone I , Tanshinone II A, Notoginsenoside RI, Ginsenoside Re, Ginsenoside Rgl, Ginsenoside Rbl, Notoginsenoside R2, Notoginsenoside R2 iso., Ginsenoside Rg2, Ginsenoside Rhl, Ginsenoside Rhl iso., Ginsenoside Rd, Ginsenoside Rd iso., Ginsenoside Rf-H2O, Notoginsenoside R2-H2O, Ginsenoside Rg6 or F4, Ginsenoside Rk3, Ginsenoside (Rh4), Ginsenoside 20(R)-Rg3, Ginsenoside 20(S)-Rg3, Ginsenoside (Rkl), Ginsenoside (Rg5) and the like.
(Table Removed)
hi the extraction process and analysis method of the present invention, fingerprint atlas was used for completely characterizing the physical and chemical properties of the Radix Salviae Miltiorrhizae and the Radix Notoginseng in the TCM preparation. Compared to the prior art in which only one or two compounds are used to represent complex biologically active ingredients in TCM preparations, this characterization means is more suitable for controlling the quality of the TCM preparations.
The biologically active ingredients in the present TCM preparations were detected using the HPLC-MS analysis method according to the present invention. As a result, 12 components from Radix Salviae Miltiorrhizae and 21 components from Radix Notoginseng have been identified in total. The compounds were identified mainly based on an analysis of the MSn data and comparison with data from literature. Finally, a large number of the components were completely confirmed with respect to their structures through comparison with the control samples. It can be concluded thereby that the analysis for the chemical composition
of the present TCM preparation using the HPLC-MS method of the present invention can produce abundant information on the structure of the biologically active ingredients. The characterization by these information for the physical and chemical properties of Radix Salviae Miltiorrhizae and Radix Notoginseng in the present TCM preparation has consequently a much better effect than those methods in the prior art.
The following tests demonstrate that the present TCM preparation has an effect on the treatment of cardiovascular and cerebrovascular diseases.
1. Effects of the TCM Preparation on Myocardial Ischemia and Myocardial Infarction in Anaesthetized Dog
An epicardial electrogram was used to map a range of myocardial ischemia and to indicate the extent thereof. Quantitative histology (N-BT staining method) was used to determine an area of myocardial infarction. Also determined were changes of blood flow of coronary artery, myocardial oxygen consumption, and activities of serum CK and LDH, and blood plasma ET, TXB2, and 6-Keto-PGFia. The TCM preparation according to the present invention was studied upon alimentary administration with regard to its effect on acute myocardial ischemia, myocardial infarction, and related indicators in test dogs.
The test results show that the TCM preparation according to the present invention has a significant effect in improving acute myocardial ischemia and myocardial infarction of dogs. It can lead to a reduced extent of myocardial ischemia (E-ST) indicated by the epicardial electrogram (PO.001 relative to the control group using normal saline), a significantly reduced area of infarction indicated through N-ST staining (P 6-Keto-PGFia/TXB2 (P 2. Effects of the TCM Preparation on Myocardial Infarction Caused by Ischemic Reperfusion
It was found through an observation on a rat model with the damage of myocardial ischemia reperfusion that, the TCM preparation according to the present invention could lead to a significantly reduced extent of myocardial damage, a decreased area of myocardial infarction (p 3. Effects of the TCM Preparation on Dynamics of Cardiac Blood Flow and Myocardial
Oxygen Consumption in Dogs
The TCM preparation of the present invention was evaluated with respect to its effect on dynamics of cardiac blood flow and myocardial oxygen consumption in anaesthetized normal dogs.
The results show that the TCM preparation according to the present invention can lead to a significantly improved blood flow of coronary artery (p 4. Effects of the TCM Preparation on Platelet Agglutination in Rabbits
The TCM preparation of the present invention was evaluated through Born nephelometry with respect to its effect on platelet agglutination in rabbits.
The results show that the TCM preparation can, upon an intragastric administration for 7 successive days, lead to a significant reduction of the platelet agglutination hi rabbits induced by arachidonic acid (AA) (p 5. Effects of the TCM Preparation on Thrombogenesis in Vitro and Blood Viscosity in Rats
The TCM preparation of the present invention was evaluated with respect to its effect on thrombogenesis in vitro and blood viscosity in rats.
The results show that the TCM preparation can, upon an intragastric administration for 7 successive days, lead to a considerably shortened thrombus (p 6. Effects of the TCM Preparation on Hyperlipidemia and Atherosclerosis in Rabbits
A hyperlipidemia and atherosclerosis (AS) model for test was established through feeding fodder with a high content of cholesterol to a rabbit. The TCM preparation of the present invention was evaluated with respect to its effect on this model.
The results show that the TCM preparation according to the present invention can lead to a significantly decreased concentration of TC, TG, LDL-C, VLDL-C in serum and a decreased TC/HDL-C ratio (pO.05-0.001 relative to the control group suffering from Hyperlipidemia) in rabbits, a significantly increased HDL-C concentration (p Hyperlipidemia). The present TCM preparation has a significant effect in improving the activity of SOD in the liver (p 7. Effects of the TCM Preparation on Localized Cerebral Ischemia in Rats
Using a rat model with a middle cerebral artery thrombosis (MCAT), the TCM preparation of the present invention was determined with respect to its effect on an area of cerebral infarction in MCAT rats.
The results demonstrate that the TCM preparation according to the present invention has a significant effect of anti-cerebral ischemia.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1 is a fingerprint atlas of the components of the Radix Salviae Miltiorrhizae in the drop pills as one of the dosage forms of the present TCM preparation. In this figure, peak 1 represents Danshensu; peak 2 represents Protocatechualdehyde; peak 3 represents Isolithospermic acid A; peak 4 represents Isolithospermic acid B; peak 5 represents Salvianolic acid D; peak 6 represents Rosmarinic acid; peak 7 represents Salvianolic acid B; and peak 8 represents Salvianolic acid A.
Figure 2 is a HPLC spectrum of the water-soluble components of the Radix Salviae Miltiorrhizae in the present TCM preparation, hi this figure, peak 1 represents Danshensu; peak 2 represents Protocatechualdehyde; peak 3 represents Isolithospermic acid A; peak 4 represents Isolithospermic acid B; peak 5 represents Salvianolic acid D; peak 6 represents Salvianolic acid E; peak 7 represents Rosmarinic acid; peak 8 represents Salvianolic acid B; peak 9 represents Salvianolic acid G; and peak 10 represents Salvianolic acid A.

Figure 3 is a MS-TIC spectrum of the water-soluble components of the Radix Salviae Miltiorrhizae in the present TCM preparation.
Figure 4 is a HPLC spectrum of the liposoluble components of the Radix Salviae Miltiorrhizae in the present TCM preparation. In this figure, peak 1 represents Tanshinone I; and peak 2 represents Tanshinone II A.
Figure 5 is a MS-TIC spectrum of the liposoluble components of the Radix Salviae Miltiorrhizae in the present TCM preparation.
Figure 6 is a MS-TIC spectrum of the components of the Radix Notoginseng in the present TCM preparation.
DETAILED DESCRIPTION OF THE EMBODIMENTS
The invention will be further illustrated in details by reference to the following examples. The examples are for illustrative purpose and are not intended to limit the scope of the invention.
EXAMPLES
Example 1 (preparation example)
41.06 g of Radix Salviae Miltiorrhizae and 8.03 g of Radix Notoginseng were weighed out, to which sodium bicarbonate was added in an amount of 1.8% based on the total weight of said medicinal materials. The resulting mixture was boiled out in 4 folds of water for 2 hours, and then in 3 folds of water for another 1 hour. After filtration, the combined filtrates were concentrated until a specific gravity of 1.19-1.20 (75±1°C) was achieved. Then, a 95% ethanol was added in an amount sufficient to obtain a 65% content of the ethanol (20°C). The mixture was subsequently allowed to stand for 12 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.37 (55-60 °C ), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed uniformly with 0.46 g of Borneol and 18 g of polyethylene glycol-6000. The mixture was melted at a temperature of 85 °C for 80 mins. The melting liquor was then introduced into the dropping tank of a drop-pill machine with the tank temperature being maintained at 86 °C, in which the liquor was dropped into a liquid paraffin at 8"C. The obtained drop pills were taken out, subjected to an oil removal and then screened through a sieve to obtain the desired preparation.
Example 2 (preparation example)
59.36 g of Radix Salviae Miltiorrhizae and 6.38 g of Radix Notoginseng were weighed out, to which potassium carbonate was added in an amount of 1.0% based on the total weight of said medicinal materials. The resulting mixture was boiled out in 4 folds of water for 2.5 hours, and then in 3 folds of water for another 1.5 hours. After filtration, the combined filtrates were concentrated until a specific gravity of 1.19-1.20 (75±1°C) was achieved. Then, a 85% ethanol was added in an amount sufficient to obtain a 70% content of the ethanol (20 °C). The mixture was subsequently allowed to stand for 10 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.35 (55-60 °C ), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed uniformly with 0.34 g of Borneol and 23 g of polyethylene glycol-6000. The mixture was melted at a temperature of 89 °C for 100 mins. The melting liquor was then introduced into the dropping tank of a drop-pill machine with the tank temperature being maintained at 85 °C, in which the liquor was dropped into a methyl silicone oil at 8"C. The obtained drop pills were taken out, subjected to an oil removal and then screened through a sieve to obtain the desired preparation.
Example 3 (preparation example)
12.60 g of Radix Salviae Miltiorrhizae and 56.15 g of Radix Notoginseng were weighed out, to which potassium bicarbonate was added in an amount of 1.0% based on the total weight of said medicinal materials. The resulting mixture was boiled out in 4 folds of water for 2.5 hours, and then in 3 folds of water for another 1.5 hours. After filtration, the combined filtrates were concentrated until a specific gravity of 1.19-1.20 (75±1"C) was achieved. Then, a 95% ethanol was added in an amount sufficient to obtain a 70% content of the ethanol (20
°C). The mixture was subsequently allowed to stand for 10 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.35 (55-60 °C), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed with 0.34 g of Borneol and 23 g of polyethylene glycol-6000. The mixture was melted at a temperature of 89 °C for 100 mins. The melting liquor was then introduced into the dropping tank of a drop-pill machine with the tank temperature being maintained at 85 °C, in which the liquor was dropped into a methyl silicone oil at 8°C. The obtained drop pills were taken out, subjected to an oil removal and then screened through a sieve to obtain the desired preparation.
Example 4 (preparation example)
31.12 g of Radix Salviae Miltiorrhizae and 9.21 g of Radix Notoginseng were weighed out, to which sodium hydroxide was added in an amount of 0.5% based on the total weight of said medicinal materials. The resulting mixture was boiled out in 4 folds of water for 1.5 hours, and then in 3 folds of water for another 1.5 hour. After filtration, the combined filtrates were concentrated until a specific gravity of 1.19-1.20 (75±1"C) was achieved. Then, a 88% ethanol was added in an amount sufficient to obtain a 66% content of the ethanol (20 °C). The mixture was subsequently allowed to stand for 10 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.40 (55-60 °C ), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed uniformly with 0.50 g of Bomeol, 90 g of mannitol, 15 g of calciumedetate sodium and 15 ml of distilled water. The resultant mixture was lyophilized, and finally formulated into injection powders.
Example 5 (preparation example)
116.35 g of Radix Salviae Miltiorrhizae and 58.21 g of Radix Notoginseng were weighed out, to which sodium bicarbonate was added in an amount of 2.0% based on the total weight of said medicinal materials. The resulting mixture was boiled out hi 4 folds of water for 2 hours, and then in 3 folds of water for 1.5 hour. After filtration, the combined filtrates were
concentrated until a specific gravity of 1.19-1.20 (75±1"C) was achieved. Then, a 88% ethanol was added in an amount sufficient to obtain a 66% content of the ethanol (20°C). The mixture was subsequently allowed to stand for 10 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.40 (55-60 °C ), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed uniformly with 1.80 g oil of Lignum Dalbergiae Odoriferae and 40 g of microcrystalline cellulose. A 3% solution of polyvidone in ethanol was added to soften the mass. The softened mass was then sieved through an 18-size mesh to form granules. The granules were dried at a temperature of 60 °C for 35 mins, trimmed, and then mixed uniformly with 4 g of talcum powders. The mixture obtained was encapsulated to obtain the desired preparation.
Example 6 (preparation example)
116.35 g of Radix Salviae Miltiorrhizae and 58.21 g of Radix Notoginseng were weighed out, to which sodium bicarbonate was added in an amount of 2.0% based on the total weight of said medicinal materials. The resulting mixture was boiled out in 4 folds of water for 2 hours, and then in 3 folds of water for 1.5 hour. After filtration, the combined filtrates were concentrated until a specific gravity of 1.19-1.20 (75±1°C) was achieved. Then, a 88% ethanol was added in an amount sufficient to obtain a 66% content of the ethanol (20 °C). The mixture was subsequently allowed to stand for 10 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.40 (55-60 °C ), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed uniformly with 0.90 g of Borneol, 120 g of microcrystalline cellulose, 40 g of hydroxypropyl methyl cellulose, 5 g of xylitol, and 2 g of magnesium stearate. The obtained mixture was compressed into tablets to obtain the desired preparation.
Example 7 (preparation example)
140.35 g of Radix Salviae Miltiorrhizae and 36.42 g of Radix Notoginseng were weighed out, to which sodium bicarbonate was added in an amount of 2.5% based on the total weight of said medicinal materials. The resulting mixture was boiled out hi 4 folds of water for 2 hours, and then hi 3 folds of water for 1.5 hour. After filtration, the combined filtrates were concentrated until a specific gravity of 1.19-1.20 (75±1"C) was achieved. Then, a 90% ethanol was added in an amount sufficient to obtain a 65% content of the ethanol (20°C). The mixture was subsequently allowed to stand for 8 hours, and the supernatant was separated. The ethanol was recovered from the supernatant, and the residue was concentrated until it had a relative density of 1.35 (55-60 °C ), which was an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng.
The above extract was then mixed uniformly with 1.00 g of Bomeol and 46 g of microcrystalline cellulose. A 3% solution of polyvidone hi ethanol was added to soften the mass. The softened mass was then sieved through an 18-size mesh to form granules. The granules were dried at a temperature of 60'C for 30 mins, trimmed, and then mixed uniformly with 4 g of talcum powders. The mixture obtained was compressed into tablets to obtain the desired preparation.
Example 8 (detection example for active component) 1. Preparation of Sample
(1) The Water-soluble Components of the Radix Salviae Miltiorrhizae hi the Present TCM
Preparation
148.4 mg was weighed out each for the TCM drop pills from example 1, 2 and 3, the TCM injection powders from example 4, the TCM capsules from example 5, the TCM oral disintegrant tablets from example 6, and the TCM tablets from example 7. Said preparations were dissolved in 6 ml of water through ultrasound for 15 mins, and then filtered through a 0.45um nylon film to obtain a yellow sample solution, respectively.
(2) The Components of the Radix Notoginseng and Liposoluble Components of the Radix
Salviae Miltiorrhizae in the Present TCM Preparation
1003.8 mg was weighed out each for the TCM drop pills from example 1,2 and 3, the TCM injection powders from example 4, the TCM capsules from example 5, the TCM oral disintegrant tablets from example 6, and the TCM tablets from example 7. Said preparations were dissolved in 10 ml of 4% aqueous ammonia through ultrasound for 15 mins, and then filtered through a 0.45um nylon film, respectively. The filtrate was pretreated on an Extract-Clean Cig (Alltech Associates, toe, U.S.) column. This sample, upon loaded into the column, was washed with 10 ml of water, and then eluted with 2 ml of methanol to obtain the test sample as a yellow eluent, respectively.
2. Analysis of Sample
(1) Instruments and Agents
Agilent Series-1100 Liquid Chromatograph (Agilent); G1315A Diode Array Detector; G1313A Automatic Sample Injector; G1316A Thermostat; G1322A Deaerator and Duplex Pump; HP Instrument Chromatographic Work Station.
Type G2445A Series 1100 LC-MSD/Trap Mass Spectrograph (Bruker); lonization was carried out by means of electro-spraying; Extract-Clean Cig Column(100mg/ml, Alltech Associates, Inc, U.S.), acetonitrile being chromatographically pure (TEDIA), water being redistilled water, and acetic acid being analytically pure.
(2) Detection Conditions of Instruments
Agilent Zorbax SB-CIS chromatographic column (Sum, 4.6mmx25cm, Agilent, SN: USCL009296) was used for HPLC analysis. The gradient elution and mass spectrum detection of each sample were performed under following conditions.
© The Water-soluble Components of the Radix Salviae Miltiorrhizae in the TCM Preparation from Each Example
HPLC Elution Conditions:
(Table Removed) MS Analysis Conditions:

(Table Removed) i The Components of the Radix Notoginseng in the TCM Preparation from Each Example
HPLC Elution Conditions:

(Table Removed) MS Analysis Conditions:

(Table Removed) 3. Analysis Results and Peak Identification
The components were identified in the following two aspects: (1) using control samples; (2) using the UV absorption properties and ion fragment information from MSn in combination with literature data
4. Identification Results
(1) The Water-soluble Components of the Radix Salviae Miltiorrhizae in the Radix Salviae Miltiorrhizae Preparation from Each Example of the Present Invention (see tables 1 and 2, and figures 2 and 3).
Table 1 HPLC-MS Data and Identification Results

(Table Removed) Table 2 HPLC-MSnData

(Table Removed) It can be seen from the MS" results that the second and third peak have very similar structures as that of lithospermic acid. They are considerably different from lithospermic acid, however, with respect to UV absorption. Lithospermic acid has a relatively strong absorption near 253 nm due to its phenyl coumaran backbone, while the second and third peak do not have such a absorption property. Both of these peaks have UV absorption very similar with that of Salvianolic acid E, which demonstrates that the two compounds corresponding to these two peaks are likely to have the same backbone as Salvianolic acid E, i.e. the structure of carboxyl diphenyl ethylene backbone. It is thereby concluded that they have structures as those of Isolithospermic acids A and B shown in the above structure formula for components.
These two structures have never been reported, and are therefore named as Isolithospermic acids A and B herein.
(2) The Liposoluble Components of the Radix Salviae Miltiorrhizae in the TCM Preparation of the Present Invention (see table 3, and figures 4 and 5).
Table 3 HPLC-MS Data and Identification Results

(Table Removed) (3) The Components of the Radix Notoginseng in the Radix Salviae Miltiorrhizae Drop Pills of the Present Invention (see tables 4 and 5, and figure 6).
Table 4 HPLC-MS Data and Identification Results

(Table Removed) TableS HPLC-MSnData

(Table Removed) Based on the above research, the extraction process and analysis method for the TCM preparation of the present invention are established, which include:
(1) a solid-phase process for extracting the liposoluble components of Radix Salviae
Miltiorrhizae and the components of Notoginsenoside from the drop pills of Radix Salviae
Miltiorrhizae',
(2) a method of HPLC-MS analysis for each sample
12 components from Radix Salviae Miltiorrhizae and 21 saponin components from Radix Notoginseng have been identified in total. Among them, 4 water-soluble components of Radix Salviae Miltiorrhizae, 2 liposoluble components of Radix Salviae Miltiorrhizae and 9 components of saponin have been identified through comparison with the control samples, while other compounds were identified mainly based on an analysis of MSn data and comparison with data from literature.
Example 9 (Detection example of the fingerprint atlas for the components of Radix Salviae Miltiorrhizae in the TCM preparation)
1. Instruments and Agents
Instruments: Agilent 1100 Liquid Chromatograph, comprising: quad-pump, online deaerating system, automatic sample injector, DAD detector, column temperature tank, Chemstation work station; BS210S electronic balance (1/10"4 g) (Beijing Sartorius Company), METTLER AE240 electronic balance ((1/10"4 g or 1/10'5 g) ( Mettler-Toledo Corporation, Shanghai), LD4-2 centrifuge (4000 r/min) (Beijing Medical Centrifuge Factory), Digital thermostatic water-bath kettle (Tianjing Changfeng Corporation), RE-52AA rotary evaporator (Shanghai Yarong Biochemical Instrumentation Factory), SHE- (III) water-circulating vacuum pump (Gongyi Yingyuyuhua Instrumentation Factory), KQ-250B ultrasonic cleanser (Kunshan Ultrasonic Instrumentation Corporation), HENGAO T&D filter(HENGGAO T&D), synthetic fiber membrane filter (aperture 0.45um)(Shanghai Xingya Purifying Materials Factory).
Agents: acetonitrile (chromatographically pure, Merck Company, US), phosphoric acid (top grade), Wahaha pure water.
2. Preparation of Test Sample
10 pills of the TCM preparation from each batch of Example 1 were weighed accurately and then introduced into a 10 ml measuring bottle. Distilled water was added to in an amount sufficient to dissolve the pills through shaking with ultrasound for 15 mins. And more distilled water was then added to achieve a volume of 10 ml. The obtained solution was subjected to centrifugation or filtration to obtain a sample solution.
3. HPLC Analysis Conditions
Agilent ZoRBAx SB-CIS (4.6*250mm, 5µm) chromatographic column; Mobile phase: mobile phase A being a 0.02% aqueous phosphoric acid solution, mobile phase B being a 80% acetonitrile-0.02% aqueous phosphoric acid solution; flow rate: l.000ml/min; detection wavelength: 280nm, column temperature: 30°C; injected sample volume: l0µl.
Elution Gradient of Mobile Phase:

(Table Removed) 4. Detection Results (see table 7)
Table 7 Detection Results for Components of Radix Salviae Miltiorrhizae in 200 Batches of Above TCM Drop Pills

(Table Removed) Note:
Peak 1 represents Danshensu; peak 2 represents Protocatechualdehyde; peak 3 represents Isolithospermic acid A; peak 4 represents Isolithospermic acid B; peak 5 represents Salvianolic acid D; peak 6 represents Rosmarinic acid; peak 7 represents Salvianolic acid B; and peak 8 represents Salvianolic acid A (see figure 1).
Table 7 shows the relative positions and ratios of area (retention time and peak area) of 8 peaks, wherein 3 peaks have a ratio of single peak area to total peak area greater than 10% and all the 8 peaks have a ratio of single peak area to total peak area greater than 2%.























We Claim:
1. A process of identification of compounds in a composition said composition for the treatment
of cardiovascular and cerebrovascular diseases, comprising Radix Salviae Miltiorrhizae, Radix
Notoginseng, sodium hydroxide, sodium bicarbonate, sodium carbonate, potassium hydroxide,
potassium bicarbonate, potassium carbonate or a mixture thereof, ethanol, Borneol or an oil of
Lignum Dalbergiae Odorferae and an excipient,
characterized in that the said composition comprises two components as described herein both having a quasi-molecular ion mass peak m/z of 537[M-H]-, a second fragment ion m/z of 493[M-H-CO2]- and 295[M-CO2-R-H2O]-, and a third fragment ion m/z of 159 and 109, with the maximum absorption wave length of 327 nm,
wherein mass spectrum analysis is carried out as follows:
(1) HPLC-MS is carried out through the negative ion detection under following conditions of dry gas flow rate: 10 L/min; nebulizer pressure: 60 psi; dry gas temperature: 350°C; capillary voltage: 3500 v; and m/z scan range: 100-1200; and
(2) HPLC-MS- is carried out through the negative ion detection under following conditions of dry gas flow rate: 10 L/min; nebulizer pressure: 60 psi; dry gas temperature: 350 °C; capillary voltage: 3500 v; m/z scan range: 100-800; and fragment amplitude: 1.5-3.0 v.
2. The process as claimed in claim 1, wherein the two components are identified as:
Isolithospermic acids A and B and having a structural formula:
(structure Removed)
3. The process as claimed in claim 1, wherein the said composition comprises the components having a quasi molecular ion mass peak m/z of 417, 717, 359, 717, 339, and 493 [M-H]-respectively, and wherein
the component with 417 [M-H]~ is Saivianoiic Acid D and has a second fragment ion m/z of 175[M-CO2-R-H2O]- and 373[M-H-CO2]-, and a third fragment ion m/z of 147, 157 and 133;
the component with 717[M-H]" is Saivianoiic Acid E and has a second fragment ion m/z of 519[M-R-H2O]- and 321 [M-2R-2H2O]-, a third fragment ion m/z of 321[M-R-H2O]-and 339[M-R]- and a third fragment ion m/z of 279, 293, 249, 223 and 185;
the component with 359 [M-H]- is Rosmarinic acid and has a second fragment ion m/z of 161 [M-R-H2O]-, 179[M-R]-,and 195;
the component with 717 [M-H]- is Saivianoiic Acid B and has a second fragment ion m/z of 519[M-R-H2O]- and 321[M-2R-2H2O]-, a third fragment ion m/z of 321[M-R-H2O]- and 339[M-R]-, and a forth fragment ion m/z of 279, 293, 249, 233 and 185;
the component with 339 [M-H]" is Saivianoiic Acid G and has a second fragment ion m/z of 321[M-H-H2O]- and 295[M-H-CO2]- a third fragment ion mz of 279 and 267, and a forth fragment ion m/z of 251; and
the component with 493 [M-H]" is Saivianoiic Acid A and has a second fragment ion m/z of 295[M-R-H2O]-, and a third fragment ion m/z of 159 and 109;
and wherein the mass spectrum analysis is carried out as follows:
(1) HPLC-MS is carried out through the negative ion detection under following conditions of dry gas flow rate: 10 L/min; nebulizer pressure: 60 psi; dry gas temperature: 350°C; capillary voltage: 3500 v; and m/z scan range: 100-1200; and
(2) HPLC-MSn is carried out through the negative ion detection under following conditions
of
dry gas flow rate: 10 L/in; nebulizer pressure: 60 psi; dry gas temperature: 350 °C; capillary voltage: 3500 v; m/z scan range: 100-800; and fragment amplitude: 1.5-3.0 v.
4. The process as claimed in claim 1, wherein the said composition comprises the components having a quasi-molecular ion mass peak m/z of 931, 945, 799, 1107, 769, 769, 783, 637, 637, 945, 945, 781, 751, 751, 765, 783, 783, 765, and 765[M-H]- respectively, wherein:
the component with 931 [M-H]" is Notoginsenoside R1 and has a fragment ion m/z of 799[M-H-Xyl]-, 637[M-H-Xyl-Glc]-, and 475[M-H-Xyl-2Glc]-;
the component with 945 [M-H]- is Ginsenoside Re and has a fragment ion m/z of 799[M-H-Rham]-, 783[M-H-Glc]- 637[M-H-Rham-Glc]-, and 475[M-H-Rham-2Glc]-;
the component with 799 [M-H]- is Ginsenoside Rg1 and has a fragment ion m/z of 637[M-H-Glc]-,and 475[M-H-2Glc]-;
the component with 1107 [M-H]- is Ginsenoside Rb1 and has a fragment ion m/z of 945 [M-H-Glc]-, 783[M-H-2Glc]-, 621 [M-H-3Glc]", and 459[M-H-4Glc]-;
the component with 769 [M-H]- is Notoginsenoside R2 and has a fragment ion m/z of 637[M-H-Xyl]-, and 475[M-H-Xyl-Glc]-;
the component with 769 [M-H]" is Notoginsenoside R2 isomer and has a fragment ion m/z of 637[M-H-Xyl]- and 475 [M-H-Xyl-Glc]-;
the component with 783 [M-H]" is Ginsenoside Rg2 and has a fragment ion m/z of 637[M-H-Rham]-, and 475[M-H-Rham-Glc]";
the component with 637 [M-H]" is Ginsenoside Rh1 and has a fragment ion m/z of 475[M-H-Glc]-;
the component with 637 [M-H]- is Ginsenoside Rh1 isomer and has a fragment ion m/z of 475[M-H-Glc]-;
the component with 945 [M-H]- is Ginsenoside Rd and has a fragment ion m/z of 783[M-H-Glc]-, 621 [M-H-2Glc]-, and 459[M-H-3Glc]-;
the component with 945 [M-H]- is Ginsenoside Rd isomer and has a fragment ion m/z of 783[M-H-Glc]-, 621 [M-H-2Glc]- and 459[M-H-3Glc]-;
the component with 781 [M-H]- is Ginsenoside Rf-H2O and has a fragment ion m/z of 619[M-H-Glc]-, and 457[M-H-2Glc]-;
the component with 751 [M-H]- is Notoginsenoside R2-H2O and has a fragment ion m/z of 619[M-H-Xyl]-;
the component with 751 [M-H].sup.- is Notoginsenoside R2-H2O and has a fragment ion m/z of 619[M-H-Xyl]";
the component with 765 [M-H]- is Ginsenoside Rg6/F4 and has a fragment ion m/z of 619[M-H-Rham]-, and 457[M-H-Rham-GIc]-;
the component with 783 [M-H]- is Ginsenoside 20R-Rg3 has a fragment ion m/z of 621[M-H-Glc]-, and 459[M-H-2Glc]-;
the component with 783 [M-H]- is Ginsenoside 20S-Rg3 has a fragment ion m/z of 621[M-H-Glc]-, and 459[M-H-2Glc]-;
the component with 765 [M-H]- is Ginsenoside Rkl has a fragment ion m/z of 603[M-H-Glc]-, and 441[M-H-2Glc]-;
the component with 765 [M-H]- is Ginsenoside Rg5 has a fragment ion m/z of 603[M-H-Glc]-, and441[M-H-2Glc]-;
wherein the mass spectrum analysis is carried out as follows:
(1) HPLC-MS is carried out through the negative ion detection under following conditions of dry gas flow rate: 10 L/min; nebulizer pressure: 60 psi; dry gas temperature: 350°C; capillary voltage: 3500 v; and m/z scan range: 400-1500; and
(2) HPLC-MS- is carried out through the negative ion detection under following conditions
of
dry gas flow rate: 10 L/min; nebulizer pressure: 60 psi; dry gas temperature: 350 °C; capillary voltage: 3500 v; m/z scan range: 400-1200; and fragment amplitude: 1.2-1.5 v.
5. The process as claimed in claim 1, wherein the physical and chemical parameters of the said composition are characterized by:
• the average retention time of 8 peaks in the HPLC spectrum: 6.04, 9.90, 16.89, 17.84, 20.31,23.74, 27.73, and 31.02,
• the Relative standard deviation % values of the retention time 0.31, 0.25, 0.61, 0.70, 0.96, 0.76, 0.50, and 1.18, respectively;
• the average peak area 1627.92, 2575.54. 366.89, 381.40, 186.08, 555.35, 281.91, and 1852.33, respectively,
• the Relative standard deviation % values of the peak area 5.91, 13.53, 10.92, 13.81, 12.04, 10.48, 18.08, and 14.84, respectively; and
• the ratio of single peak area to total peak area accounts for 19.6%-22.0%, 28.5-37.4%, 4.2%-5.2%, 4.2%-5.5%, 2.1%-2.7%, 6.4%-7.8%, 3.0%-4.3%, and 20.2%-27.2%, respectively;
wherein the above physical and chemical parameters are determined under the following conditions:
(1) high performance liquid chromatography:
filler: octadecylsilyl-silica gel; flow rate: 1.000 ml/min; and detection wavelength: 280 nm;
and
(2) elution:
mobile phase A: 0.02% aqueous phosphoric acid solution; mobile phase B: 80% acetonitrile-0.02% aqueous phosphoric acid solution; and gradient elution: mobile phase A being changed from 90% to 78% homogeneously and mobile phase B being changed from 10% to 22% homogeneously during 0 to 8 min, mobile phase A from 78% to 74% and mobile phase B from 22% to 26% during 8 to 15 min, and mobile phase A from 74% to 48% and mobile phase B from 26% to 52% during 15 to 55 min;
and wherein the sample solution is prepared and determined through the following steps of: weighing 10 pills of the preparations accurately and adding them into a 10 ml measuring bottle; adding distilled water at an amount sufficient to dissolve the preparations through shaking with ultrasound for 15 minutes; adding more distilled water to achieve a volume of 10 ml; subjecting the resultant solution to centrifugation or filtration to obtain the sample solution; injecting an accurate 10 |il of the sample solution into the HPLC system; and determining the sample solution in accordance with HPLC chromatography to obtain the HPLC spectrum.
6. The process as claimed in claim 5, wherein the 8 peaks with average retention time of 6.04, 9.90, 16.89, 17.84, 20.31, 23.74, 27.73 and 31.02 correspond to Danshensu, Protocatechualdehyde, Isolithospermic acid A, Isolithospermic acid B, Salvianolic acid D, Rosmarinic acid, Salvianolic acid B, and Salvianolic acid A, respectively.
7. A method for preparing the composition of claim 1, comprising the steps of:

• weighing Radix Salviae Miltiorrhizae and Radix Notoginseng;
• adding sodium hydroxide, sodium bicarbonate, sodium carbonate, potassium hydroxide, potassium bicarbonate, potassium carbonate or a mixture thereof in an amount of 0.5%-4.0% based on the total weight of said medicinal materials;
• boiling the resulting mixture out in 3-6 folds of water for 2-4 times;
• subjecting the mixture to filtration and concentrating the combined filtrates;
• adding an ethanol with -the concentration above 70% in an amount sufficient to achieve a 65-70% content of the ethanol;
• allowing the mixture to stand and separating the supernatant;
• recovering the ethanol from the supernatant, and concentrating the residue until it has a relative density of 1.20-1.50, which is an extract of Radix Salviae Miltiorrhizae-Radix Notoginseng;
• mixing the above extract with Borneol or an oil of Lignum Dalbergiae Odorferae; and adding an excipient to obtain the preparation.

8. The method for preparation as claimed in claim 7, wherein the said excipient is starch, dextrin, lactose, microcrystalline cellulose, hydroxypropyl methyl cellulose, polyethylene glycol, magnesium stearate, micro silicon gel, xylitol, lactitol, glucose, glycine, mannitol, methyl starch sodium, cross-linked sodium carboxyl methyl cellulose, cross-linked polyvinylpyrrolidone as such or in the water, or a mixture of more than one of the above adjutants; said preparation is in the dosage form of injection, tablet, sustained-release tablet, drop pill, granule, injection powder, capsule, microgranule, or oral disintegrant.
9. The method for preparation as claimed in claim 8, wherein the said preparation is in the dosage form of drop pill.
10. The method for preparation as claimed in claim 9, comprising the steps of:
weighing Radix Salviae Miltiorrhizae and Radix Notoginseng;
adding sodium bicarbonate in an amount of 1.4% - 1.9% based on the total weight of said medicinal materials;
boiling the resulting mixture out in 4-5 folds of water for 2-3 hours, and then in 3-4 folds of water for 1 -2 hours;
subjecting the mixture to filtration and concentrating the combined filtrates until a specific gravity of 1.16-1.20 is achieved;
adding an ethanol with the concentration above 70% in an amount sufficient to obtain a 65-70% content of the ethanol;
allowing the mixture to stand for 8-12 hours and separating the supernatant;
recovering the ethanol from the supernatant, and concentrating the residue until it has a relative density of 1.32-1.40, which is an extract of Radix Salviae Miltiorrliizae-Radix Notoginseng;
mixing the above extract with Borneol or an oil of Lignum Dalbergiae Odorzferae, and polyethylene glycol-6000 uniformly;
heating the mixture to melting;
dropping the melt into a coolant of liquid paraffin or methyl silicone oil through a drop-pill machine;
taking out the obtained drop pills and subjecting them to an oil removal; and sieving the pills to obtain the desired preparation.

Documents:

5739-DELNP-2006-Abstract (03-11-2009).pdf

5739-DELNP-2006-Abstract-(19-05-2010).pdf

5739-delnp-2006-abstract.pdf

5739-DELNP-2006-Claims (03-11-2009).pdf

5739-DELNP-2006-Claims-(13-08-2009).pdf

5739-DELNP-2006-Claims-(19-05-2010).pdf

5739-delnp-2006-claims.pdf

5739-DELNP-2006-Correspondence-Others (03-11-2009).pdf

5739-DELNP-2006-Correspondence-Others-(13-08-2009).pdf

5739-DELNP-2006-Correspondence-Others-(19-05-2010).pdf

5739-delnp-2006-correspondence-others-1.pdf

5739-delnp-2006-correspondence-others.pdf

5739-DELNP-2006-Description (Complete) (03-11-2009).pdf

5739-delnp-2006-description (complete).pdf

5739-delnp-2006-drawings.pdf

5739-DELNP-2006-Form-1 (03-11-2009).pdf

5739-DELNP-2006-Form-1-(19-05-2010).pdf

5739-delnp-2006-form-1.pdf

5739-delnp-2006-form-18.pdf

5739-DELNP-2006-Form-2 (03-11-2009).pdf

5739-DELNP-2006-Form-2-(19-05-2010).pdf

5739-delnp-2006-form-2.pdf

5739-DELNP-2006-Form-3 (03-11-2009).pdf

5739-DELNP-2006-Form-3-(13-08-2009).pdf

5739-delnp-2006-form-3.pdf

5739-delnp-2006-form-5.pdf

5739-delnp-2006-gpa.pdf

5739-delnp-2006-pct-210.pdf

5739-delnp-2006-pct-304.pdf


Patent Number 243433
Indian Patent Application Number 5739/DELNP/2006
PG Journal Number 43/2010
Publication Date 22-Oct-2010
Grant Date 18-Oct-2010
Date of Filing 03-Oct-2006
Name of Patentee TIANJIN TASLY PHARMACEUTICAL CO. LTD
Applicant Address 1 LIAOHE EAST ROAD, XINYIBAI AVENUE, BEICHEN DISTRICT, TIANJIN 300402, CHINA
Inventors:
# Inventor's Name Inventor's Address
1 CHENG YIYU 1 LIAOHE EAST ROAD, XINYIBAI AVENUE, BEICHEN DISTRICT, TIANJIN 300402, CHINA
2 ZHANG HAIJIANG 1 LIAOHE EAST ROAD, XINYIBAI AVENUE, BEICHEN DISTRICT, TIANJIN 300402, CHINA
3 YE ZHENGLIANG 1 LIAOHE EAST ROAD, XINYIBAI AVENUE, BEICHEN DISTRICT, TIANJIN 300402, CHINA
PCT International Classification Number A61K 31/045
PCT International Application Number PCT/CN2005/000333
PCT International Filing date 2005-03-17
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 200410018758.4 2004-03-17 China