Title of Invention

A BIO-FUNGICIDE AND A PROCESS FOR PREPARATION THEREOF FOR CONTROL OF POWDERY MILDEW DISEASE IN MULBERRY

Abstract A biofungicide and a process for preparation thereof for control of powdery mildew disease in mulberry. This invention relates to a process for preparation of a biofungicide for control of powdery mildew disease in mulberry comprises steps of :-pulverization of chopped plant material mixed with sterile cool water so as to obtain a paste and addition of preservative solution to the paste thus obtained. Further according to this invention there is provided a biofungicide for control of powdery mildew disease in mulberry comprising of plant material, sterile water, preservative (a) and preservative (b) in a ratio of 1: 0.1 : 4.003 ± 1.5*03 : 7.04 ± 2. -°4.
Full Text FIELD OF INVENTION
This invention relates to a biofungicide and a process for preparation thereof for control of powdery mildew disease in mulberiy.
PRIOR ART
Mulberry is cultivated for its foliage which forms sole feed for silkworms. Feeding of healthy leaves to silkworms influences the production of good cocoon crop. The pathogen Phyllactinia corylea (Pres.) Karst, causes powdery mildew disease on mulberry foliage during August to February resulting in 15-20% crop loss. The use of chemical fungicides to control mildew results in limitations viz., residual toxicity, environmental pollution, development of resistance in pathogen and higher cost of fungicides.
Currently powdery mildew disease is controlled by spraying chemical fungicides viz., Bavistin and Captan of 0.2% con., twice in a crop period. A quantum of 1.125 kg of fungicide is required for spray to one ha, of

mulberry garden. The following disadvantages are associated with the known method.
i. The synthetic fungicides used have residual toxicity to
silkworms and the sprayed leaves have to be fed to silkworms
after 15 days, ii. Synthetic fungicides destroy the non-target beneficial
phylloplane microorganisms, iii. The use of chemical/synthetic fungicides causes pollution and
is not ecofriendly.
OBJECTS OF THE INVENTION
An object of this invention is to propose a biofungicide and a process for preparation thereof for control of powdery mildew disease in mulberry.

Another object of this invention is to propose a biofungicide and a process for preparation thereof for control of powdery mildew disease in mulberry, which reduces the use of synthetic fungicides, cost incurred towards fungicides, residual toxicity and pollution caused due to application of fungicide.
Still another object of this invention is propose a biofungicide and a process for preparation thereof for control of powdery mildew disease in mulberry which is easily accessable, non phytotoxic with no residual toxicity to silkworms and cost effective formulation for control of powdery mildew on mulberry.
STATEMENT OF INVENTION
According to this invention there is provided a process for preparation of a biofungicide for control of powdery mildew disease in mulberry comprises steps of : -pulverization of chopped plant material mixed with sterile cool water so as to obtain a paste and addition of preservative solution to the paste thus obtained

Further according to this invention there is provided a biofungicide for control of powdery mildew disease in mulberry comprising of plant material, sterile water, preservative (a) and preservative (b) in a ratio of 1: 0.1 : 4.0"03 ± 1.503 : 7.-°4 ±2.~04-
BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
Further objects and advantages of this invention will be more apparent from the ensuing description when read in conjunction with the accompanying drawings and wherein:
Fig. 1 shows: Variations in the growth of mulberry plants.
Fig. 2 shows: Luxuriant growth of mulberry in the garden sprayed with seri-mildewguard.
Fig. 3 shows: Robust and luxuriant growth of mulberry garden treated with seri-mildewguard.
Fig. 4 shows: Plant growth during 2nd spray of seri-mildew guard.

Fig. 5 shows: Cocoon yield with the use of seri-mildewguard in the garden.
DETAILED DESCRIPTION OF THE INVENTION WITH REFERENCE TO THE ACCOMPANYING DRAWINGS
The present invention provides a process for preparation of a bio fungicide for control of powdery mildew disease in mulberry wherein the herbaceous phytomember of family Apiaceae commonly known as carrot family grown robustly for a period of 35 to 40 days. This has to be harvested before blossoming and used for Seri-Mildewguard preparation. The plant material is cleaned by removing roots and soil particles (if any). Then, the tender part of the plant is collected and weighed.
The plant material is washed twice in clean water and then in sterilized water.

The material is chopped into small bits of 1-2 cm and pulverized in a mixer grinder/pulverizer at a temperature range of 15 - 25°C under normal pressure. During grinding, the material is mixed with sterile cool water in a quantity of not more than 0.08 or 8/100 part by weight of actual plant material used.
The so obtained paste/semi solid material is mixed with preservative solution containing (a) Benzamidazole (Antifungal) (b) Streptocycline (Antibiotics) in sterile cool water upto a quantity of not exceeding 0.02 or 2/100 part by weight of actual unit g. plant material used.
Ratio : Plant material : Sterile water : Preservative a : Preservative b (1 : 0.1 : 4.0"03 ± 1.5"03 : 7.-4 ± 2."04)
The maximum amount of water in the biofungicide is l/10th of plant material used.
For packing, 500 g. of net active plant extract/Seri-Mildewguard 55 g. of product mix must be packed. This product is mixed and diluted with 25 Lt. of water and filtered before spraying/application.

TEST RESULTS
1) Efficacy of Seri-Mildewguard in control of powdery mildew in vio.


2) Influence of Seri-Mildewguard on mulberry leaf productivity (in tons/ha)

** Significant
Now, reference may be made to flow chart of process for use of SERI-mildewguard (per hectare of mulberry).

Quantum of application: A quantum of 11.25 kg of Seri-Mildewguard has to be sprayed per hectare/crop in two split doses at 10 days intervals. The first spray of 5 kg of Seri-Mildewguard in 250 ltrs. of water at 5-6 leaves /shoot (25-27 days old) after pruning has to be carried out. The second spray of 6.25 kg of Seri-Mildewguard in 313 ltrs. of water after 10 days of first spray or 10-12 leaves/shoot (35-37 days old) has to be carried out. Spraying has to be made during cooler hours of the non-rainy days.
Season of application: Seri-Mildewguard has to be used during winter season i.e. August to February.
Target group of consumption: Sericulturists are the target group of consumers.
Specification of raw materials: The robustly grown plant material aged about 35-40 days, but before blossoming has to be used for the preparation of Seri-Mildewguard.

HDPE bags made of good quality "A" grade milky white polythene bag having 300 gauge thickness and double sealed at one end. Bag size ranging from 16 x 24 cm or 16 x 26 cm to pack about 670 g. of product.
The preservative comprising of an antifungal and antibiotics is mixed with water and added to Seri-Mildewguard to increase the shelf life of the plant extract.
It is to be noted that the present invention is susceptible to modifications, adaptations and changes by those skilled in the art. Such variant embodiments employing the concepts and features of this invention are intended to be within the scope of the present invention, which is further set forth under the following claims:-


WE CLAIM:
1. A process for preparation of a biofungicide for control of
powdery mildew disease in mulberry comprises steps of :
- pulverization of chopped plant material mixed with sterile cool water so as to obtain a paste and
- Addition of preservative solution to the paste thus obtained.

2. A process as claimed in claim 1 wherein the plant material before being pulverized is chopped into small bits of l-2cm.
3. A process as claimed in claim 1 or 2 wherein the pulverization is carried out at 15 ~ 25°C under normal pressure.
4. A process as claimed in any of the preceding claims wherein the sterile cool water is in an amount of not more than 0.08 or 8/100 part by weight of actual plant material used during grinding.

5. A process as claimed in any of the preceding claims wherein the preservative solution contains (a) Benzamidazole and Streptocycline in sterile cool water upto a quantity of not more than 0.02 or 2/100 part by weight of actual unit g. plant material used.
6. A process as claimed in any of the preceding claims wherein the plants are subjected to washing such as herein described before chopping,
7. A process as claimed in any of the preceding claims wherein the plant is selected from herbaceous phytomember of family Apiaceae commonly known as carrot family.
8. A biofungicide for control of powdery mildew disease in mulberry comprising of plant material, sterile water, preservative (a) and preservative (b) in a ratio of 1: 0.1 : 4.003 ± 1.5"03 : 7.-04 ± 2.~04-

9. A biofungicide as claimed in claim 8 wherein preservative (a) is
Benzamidazole and (b) is Streptocycline.
10. A biofungicide and a process for preparation thereof for control of powdery mildew disease in mulberry substantially as herein described with reference to accompanying drawings.


Documents:

0039-che-2006-abstract.pdf

0039-che-2006-claims.pdf

0039-che-2006-correspondnece-others.pdf

0039-che-2006-description(complete).pdf

0039-che-2006-description(provisional).pdf

0039-che-2006-drawings.pdf

0039-che-2006-form 1.pdf

0039-che-2006-form 26.pdf

0039-che-2006-form 5.pdf

0039-chenp-2006 abstract duplicate.pdf

0039-chenp-2006 claims duplicate.pdf

0039-chenp-2006 description(complete) duplicate.pdf

0039-chenp-2006 drawings duplicate.pdf

39-che-2006 amanded claims 05-05-2010.pdf

39-CHE-2006 AMANDED CLAIMS 25-01-2010.pdf

39-CHE-2006 AMANDED PAGES OF SPECIFICATION 05-05-2010.pdf

39-CHE-2006 CORRESPONDENCE OTHERS 25-01-2010.pdf

39-CHE-2006 CORRESPONDENCE-OTHERS 15-02-2010.pdf


Patent Number 241508
Indian Patent Application Number 39/CHE/2006
PG Journal Number 29/2010
Publication Date 16-Jul-2010
Grant Date 08-Jul-2010
Date of Filing 09-Jan-2006
Name of Patentee KARNATAKA STATE SERICULTURE RESEARCH AND DEVELOPMENT INSTITUTE (KSSR&DI)
Applicant Address THALAGHRRAPURA BANGLORE 560 062.
Inventors:
# Inventor's Name Inventor's Address
1 DR. N. R. PRASAD KARNATAKA STATE SERICULTURE RESEARCH AND DEVELOPMENT INSTITUTE (KSSR&DI) THALAGHRRAPURA, BANGLORE 560 062
2 MR. J. SUKUMAR KARNATAKA STATE SERICULTURE RESEARCH AND DEVELOPMENT INSTITUTE (KSSR&DI) THALAGHRRAPURA, BANGLORE 560 062
3 S. D. PADMA KARNATAKA STATE SERICULTURE RESEARCH AND DEVELOPMENT INSTITUTE (KSSR&DI) THALAGHRRAPURA, BANGLORE 560 062
4 DR. U. D. BONGALE KARNATAKA STATE SERICULTURE RESEARCH AND DEVELOPMENT INSTITUTE (KSSR&DI) THALAGHRRAPURA, BANGLORE 560 062
PCT International Classification Number A01N25/00
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA