|Title of Invention||
"A PHARMACEUTICAL COMPOSITION COMPRISING AN AROMATASE INHIBITOR,A PROGESTIN AND AN OESTROGEN"
|Abstract||A pharmaceutical composition comprising an aromatase inhibitor, a progestin and an oestrogen in the following ranges with reference to ethinyl oestradiol at 0.01mg/day having a value of 1: the aromatase inhibitor is anastrozole in the range 10-1000; the progestin is levonorgestrel in the range 5-15; and the oestrogen is ethinyl oestradiol in the range 1-6.|
|Full Text||The present invention relates to a pharmaceutical composition comprising an aromatase inhibitor, a progestin and an oestrogen
The present invention relates to a method of treating endometriosis using a combination of an aromatase inhibitor, a progestin and an oestrogen. The invention also relates to pharmaceutical formulations comprising said combination.
Endometriosis is characterised by the presence of endometrial-like tissue outside the uterine cavity and is largely a condition seen in premenopausal women. It is estimated to affect between 2-10% of women of child-bearing age. This endometrial-like tissue responds in the same way as the normal endometrium to changes in the hormonal environment during the menstrual cycle so that as the concentrations of oestrogen and progesterone change, the tissue grows and is shed in the same way as the endometrium itself. Symptoms of endometriosis include pelvic pain, dysmenorrhoea and dyspareunia and it is often found in association with infertility although its exact relationship to infertility, except in severe endometriosis, is uncertain. [For a general review of endometriosis and current treatment strategies the user is referred to: Olive DL. Pritts EA. (2001) New England Journal of Medicine. 345(4):266-75]. Both medical and surgical approaches and a combination of the two are used in an attempt to eradicate the disease, however, in many instances treatment is not curative and the disease and its associated symptoms return.
Oestrogen is the most well-defined mitogen that enhances growth and inflammation in this extra-uterine tissue giving rise to increased pelvic pain. Treatments that inhibit the production of the major source of oestrogen in premenopausal women, namely the ovaries, or which create a so-called pseudomenopause have been used to successfully treat endometriosis. In the former class, the gonadotrophic hormone releasing hormone agonist analogues ( GnRH analogues) inhibit the production of LH and to a lesser extent FSH from the pituitary gland leading to inhibition of the production of oestradiol by the ovaries. This lowering of circulating oestradiol concentrations into the postmenopausal range results in an improvement of pelvic pain and pressure symptoms and regression of endometrial implants. Danazol and progestogenic agents are also used to treat the disease. Treatment with the GnRH analogues although effective is limited to 6 montlis because of the potential effects on bone mineral density. Treatment with danazol is also limited because of its androgenic side-effects.
Not infrequently, patients may not tolerate and/or respond to currently available medical approaches. Additionally, there is a high incidence of recurrence.
For example, 18 months after completing a 6-month course of Lupron™-depot (which
contains the GnRH agonist leuprolide), only 52% of patients had significant relief of pain.
The recurrence rate of pain in the rest of the patients was approximately 5-20% per year
(reaching a cumulative average rate at 5 years as high as 53%). The recurrence rate at 5 years
was as high as 75% in severe forms of endometriosis [see Rice VM (2002) Annals of the New
York Academy of Sciences 955:343-352]. In women treated for pelvic pain, the symptoms
usually return rather quickly after cessation of therapy. For a period of time after medical
treatment, however, the intensity of symptoms is less severe. The recurrence rates after
treatment with GnRH agonists are similar to those after danazol, and both are similar to those
obtained with surgical excision.
Recent work has demonstrated that other sources of oestrogen, in addition to the
ovaries, contributes to the development and continued presence of endometriosis. In
particular, high levels of local aromatase activity and oestrogen production within the
endometriotic tissue per se appear central to the maintenance and pathophysiology of
endometriosis. Thus, aromatase inhibitors have been suggested for the treatment of
endometriosis [Bulun et al (2000) Human Reproduction Update 6(5), 413-418; Bulun et al
(2000) Trends in Endocrinology and Medicine 11(1), 22-27].
Given continuously GnRH analogues by inhibiting the production of gonadotrophins
from the pituitary gland, reduce circulating oestradiol concentrations into the postmenopausal
range. Aromatase inhibitors would not be expected to lower serum oestradiol concentrations,
however, in pre-menopausal women because of the feedback mechanism, the hypothalamicpituitary
axis, so that when circulating oestradiol concentrations fall the pituitary gland
produces more gonadotropins, which in turn stimulate the production of oestrogen by the
ovaries. It has been hypothesised that the increased concentrations of the gonadotrophin,
follicle stimulating hormone (FSH) may result in the formation of cysts in the ovary. The
concomitant use of a progestin agent with the aromatase inhibitor would be expected to
diminish this surge in pituitary gonadotropins. Another benefit of the concomitant
administration of a progestin is that it would be expected to directly cause thinning of the
eutopic endometrium due to the dominant progestogenic effect.
Although oestrogen has a pathological role in endometriosis, it also has a protective
effect in a number of tissues, such as bone. Thus, treatment of endometriosis with oestrogen
lowering therapies may have side effect due to inhibition of the protective effects of oestrogen
in addition to inhibition of the pathological effects of oestrogen. For examples treatment of
endometriosis with GnRH analogues leads to castrate levels of oestrogen resulting in side
effects including transient vaginal bleeding, hot flashes, vaginal dryness, decreased libido,
breast tenderness, insomnia, depression, irritability and fatigue, headache, osteoporosis, and
decreased elasticity of the skin.
Thus, strategies that inhibit the oestrogen driven symptoms of endometriosis with
minimal side effects are required. The combination of an aromatase inhibitor with a progestin
would be expected to have some advantage in relation to bone loss. However, the further
addition of an oestrogen to this combination would not only prevent bone loss more
effectively than the addition of a progestin alone, but would also be expected to stop
breakthrough bleeding commonly seen with progestins. However, the addition of an
oestrogen to patients with endometriosis in combination with the partial oestrogen-reducing
therapy of an aromatase inhibitor in premenopausal women would be expected to exacerbate
the condition or reduce the efficacy of treatment with the aromatase inhibitor. Indeed, using
an endometriosis model in studies in wild type mice and aromatase knockout mice Fang et al
found that the addition of oestrogen to the aromatase inhibitor letrozole resulted in increases
in the endometriotic lesion sizes and thus exacerbates the endometriosis [Fang et al (2002)
Journal of Clinical Endocrinology & Metabolism 87(7), 3460-3466]. We have studied the
combination of an aromatase inhibitor., a progestin and an oestrogen and we have surprisingly
found that the addition of an oestrogen to the combination of an aromatase inhibitor and a
progestin, contrary to expectations, results in effective palliation of symptoms in women with
severe endometriosis. Furthermore, this combination therapy was well tolerated with only
mild hot flashes, breakthrough spotting and no significant changes between baseline and posttreatment
hip and spine bone densitometry measurements.
Thus, according to the first aspect of the invention there is provided a method of
treatment of endometriosis comprising the administration of a combination of an aromatase
inhibitor, a progestin and an oestrogen.
According to a further aspect of the invention there is provided the use of a
combination of an aromatase inhibitor, a progestin and an oestrogen in the manufacture of a
medicament for the treatment of endometriosis.
According to a further aspect of the invention there is provided a pharmaceutical
composition comprising a combination of an aromatase inhibitor, a progestin and an
oestrogen in admixture with a pharmaceutically-acceptable diluent or carrier for the treatment
For the avoidance of doubt in such a pharmaceutical composition the components may
be formulated as follows:
(i) the aromatase inhibitor, progestin and oestrogen mixed together in a single formulation;
(ii) two of the components mixed together in a single formulation and one component
formulated separately, for simultaneous or sequential dosing; or
(iii) each component formulated separately, for simultaneous or sequential dosing.
In this specification an aromatase inhibitor is defined as a compound that prevents
oestrogens from being formed from their metabolic precursors by inhibiting the enzyme
aromatase. Examples of aromatase inhibitors include:
(i) the testolactone (17a-oxa-D-homoandrost-l,4-diene-3,17-dione) that is described in the
"Journal of Clinical Endocrinology and Metabolism," 49, 672 (1979);
(ii) the compounds androsta-4,6-diene-3,17-dione, androsta-4,6-dien-17.beta.-ol-3-one
acetate, androsta-l,4,6-triene-3,17-dione, 4-androstene-19-chloro-3,17-dione, 4-
androstene-3,6,17-trione that are described in "Endocrinology" 1973, Vol. 92, No. 3,
(iii) the 19-alkynylated steroids that are described in German patent application number
(iv) the 10-(l,2,-propadienyl)-steroids that are described in German patent application number
(v) the 19-thio-androstane derivatives that are described in European patent application,
publication no. EP 100566,
(vi) the 4-androsten-4-ol-3,17-dione and its esters that are described in "Endocrinology" 1977,
Vol. 100, No. 6, page 1684 and U.S. Pat. No. 4,235,893,
(vii) the l-memyl-15.alpha.-alkyl-androsta-l,4-diene-3,17-dione that is described in German
patent application number DE 3539244,
(viii) the 10.beta.-alkinyl-4,9(l l)-estradiene derivatives that are described in German patent
application number DE 3644358 and
(ix) the l,2.beta.-methylene-6-methylene-4-androstene-3,17-dione that is described in
European Patent Application EP 250 262.
Further examples of aromatase inhibitors include: atamestane, formestane, fadrozole,
letrozole, pentrozole, anastrozole and vorozole.
A more preferred aromatase inhibitor is anastrozole. Anastrozole can be administered
at a dose and/or schedule to deliver between 0.1 and 10 mg/day, preferably, between 0.5 and
5 mg/day, most preferably anastrozole is administered at 1 mg/day.
In this specification a progestin is defined as a natural or synthetic progestational
substance that mimics some or all of the actions of progesterone. Examples of progestins
include derivatives of 19-nortestosterone, such as oestranes and gonanes, and derivatives of
17cc-acetoxyprogesterone (pregnanes). Examples of oestranes include: norethindrone and its
acetates, and ethynodiol diacetate. Examples of gonanes include norgestrel and levonorgestrel
and the less androgenic derivatives of levonorgestrel such as desogestrel, norgestimate, and
gestodene. Further examples of progestins include norgestrel, levonorgestrel, norethindrone.,
norethindrone acetate, desogestrel, norgestimate and ethynodiol diacetate. A preferred
progestin is levonorgestrel. Levonorgestrel can be administered at a dose and/or schedule to
deliver between 0.05 and 0.15 mg/day, preferably 0.1 mg/day.
hi this specification oestrogens are defined as compounds which has agonist activity at
the oestrogen receptor. Partial agonists and full agonists are envisaged but full agonists are
preferred. An example of a partial agonist is tamoxifen. Examples of full agonists include
oestrogen, oestradiol, mestranol and ethinyl oestradiol. Further examples of full agonists
include oestrogen, oestradiol and ethinyl oestradiol. Preferred oestrogens include ethinyl
oestradiol and mestranol. A more preferred oestrogen is ethinyl oestradiol. Ethinyl oestradiol
can be administered at a dose and/or schedule to deliver between 0.01 and 0.06 mg/day,
Conveniently the progestin and oestrogen components of the combination of the
invention could be provided by a combination birth control pill comprising an oestrogen and a
progestin. Examples of such birth control pills include tablets comprising:
(i) ethinyl oestradiol and norethindrone;
(ii) ethinyl oestradiol and norgestimate;
(iii) ethinyl oestradiol and desogestrel;
(iv) ethinyl oestradiol and levonogestrel;
(v) ethinyl oestradiol and gestodene;
(vi) ethinyl oestradiol and norgestrel; and
'vii) mestranol and norethindrone.
A preferred combination birth control pill comprises ethinyl oestradiol and
In one aspect of the invention, the administration is carried continuously, for at least
6 months. However, treatment for 1 to 2 years is also envisaged. Treatment for greater than
2 years is also contemplated.
Treatment of pre-menopausal women is most preferred, however treatment of
post-menopausal women would also be contemplated in those women for whom oestrogen
replacement therapy would be considered yet currently thought contraindicated due to
concerns regarding endometriosis.
In another aspect the combination of the invention is contemplated for patients who
are refractory to a combination of surgical resection and/or one or more course of prior or
subsequent hormonal treatment. Examples of such hormonal therapy include treatment with
The term 'surgical resection' refers to surgical removal of endometriotic implants
and/or lysis of adhesions caused by scarring from endometriosis. Such removal could
comprise ablation by for example a laser.
In order to carry out the invention the combination could be provided in a single
formulation or in multiple formulations, comprising one or more of the components of the
combination. Administration of the elements of the combination could be administered
simultaneously or each component could be administered at different times. Simultaneous
administration is preferred. The combination may be provided in various formulations such
as parentally (e.g. aqueous or oily suspensions) or orally (e.g., tablets, powders, capsules,
granules, aqueous or oily suspensions). Preferably, the combination is provided in an orally
available formulation to be administered daily. However, slow release formulation or depot
or transdermal formulations could also be used to administer the combination.
Thus, according a further feature of the invention there is provided a pharmaceutical
formulation comprising an aromatase inhibitor, a progestin and an oestrogen, preferably a
pharmaceutical formulation for the treatment of endometriosis.
For preparing pharmaceutical formulations of the invention, inert, pharmaceutically
acceptable carriers can be added to the components of the composition which can either be
solid or liquid. Solid form preparations include powders, tablets, dispersible granules,
capsules and cachets.
A solid carrier can be one or more substances which ma)' also act as diluents, flavoring
agents, solubilizers, lubricants, suspending agents, binders, or tablet disintegrating agents; it
can also be an encapsulating material.
In powders, the carrier is a finely divided solid which is in a mixture with the finely
divided active component. In tablets, the active component is mixed with the carrier having
the necessary binding properties in suitable proportions arid compacted in the shape and size
Suitable carriers include magnesium carbonate, magnesium stearate, talc, lactose,
sugar, pectin, dextrin, starch, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, a
low-melting wax, cocoa butter, and the like.
The term formulation is intended to include the mixture of the active component with
encapsulating material as a carrier providing a capsule in which the active component (with or
without other carriers) is surrounded by a carrier which is thus hi association with it.
Similarly, cachets are included. Tablets, powders, cachets, and capsules can be used as solid
dosage forms suitable for oral administration.
Liquid form compositions include solutions, suspensions, and emulsions. Sterile water
or water-propylene glycol solutions of the active compounds maybe mentioned as an
example of liquid preparations suitable for parenteral administration. Liquid compositions can
also be formulated in solution in aqueous polyethylene glycol solution. Aqueous solutions for
oral administration can be prepared by dissolving the active component in water and adding
suitable colorants, flavoring agents, stabilizers, and thickening agents as desired. Aqueous
suspensions for oral use can be made by dispersing the finely divided active component in
water together with a viscous material such as natural synthetic gums, res his, methyl
cellulose, sodium carboxymethyl cellulose, and other suspending agents known to the
pharmaceutical formulation art.
The pharmaceutical formulation can be in unit dosage form. In such form, the
composition is divided into unit doses containing appropriate quantities of the active
component. The unit dosage form can be a packaged preparation, the package containing
discrete quantities of the preparations, for example, packeted tablets, capsules, and powders in
vials or ampoules. The unit dosage form can also be a capsule, cachet, or tablet itself, or it can
be the appropriate number of any of these packaged forms.
The invention will now be illustrated with reference to the following non-limiting
A clinical trial was designed to assess the efficacy of a combination of an aromatase
inhibitor, a progestin and an oestrogen in the treatment of endornetriosis. 18 patients with
severe endometriosis-related pelvic pain, who previously have not responded to a
combination of surgical resection and one or more courses of hormonal treatment were
included in the trial. The patients had an age range of 23 to 46, and all patients had normal
ovarian function. These patients were treated with the oral aromatase inhibitor, anastrozole
[Arimidex™] at 1 nig/day in combination with an oestrogen-containing birth control pill,
Alesse 21™ one tablet per day [Alesse 21™ contains levonorgestrel (O.lmg), aprogestin and
ethinyl oestradiol (0.02mg), an oestrogen]. Patients were assessed for severity of pain and for
severity of side effects. Of these 18 patients, 9 patients completed the study.
A self-assessment visual analog pain scoring system (VAS), varying in severity from
0 (no pain) to 10 (maximum pain) was used, which was recorded daily. All patients had
baseline pain scores between 7 and 10. Tables 1 and 2 show the results of the pain
measurements in the trial. Table 1 shows the data for the 9 patients who completed the study,
Table 2 shows the data for all the patients in the study, the number of patients at each time
point is indicated. The results show a significant decrease in pain in the patients at each time
point, with an increase in pain relief up to the final 6 month tune point.
All results showed statistical significance between each other.
Side effects were captured in the scheduled office visits and recorded in patient charts.
Potential side effects which were monitored included lack of tolerability (i.e. lack of
hypoestrogenemic symptoms, for example those associated with GnRH treatment such as
transient vaginal bleeding, hot flashes, vaginal dryness, decreased libido, breast tenderness,
insomnia, depression, irritability and fatigue, headache, osteoporosis, breakthrough spotting
and decreased elasticity of the skin) and safety issues (i.e. no surge in gonadotropins leading
to ovarian stimulation and cyst formation and preserved bone densitometry measurements).
The most consistently observed side effects were mild hot flashes and breakthrough
spotting; although breakthrough spotting occurred in a few patients almost exclusively as a
result of inadvertent interruptions of the oestrogen-containing birth control pill.
No significant changes were detected between baseline and post treatment DEXA bone
densitometry measurements of the hip and spine.
Monthly measurements of FSH, LH, oestradiol and oestrone did not show significant
alterations from the baseline. Thus no surge in gonadotropins was observed.
Thus, the combination of an aromatase inhibitor, oestrogen and progestin results in an
efficaceous treatment of the symptoms of endometriosis, with a relatively benign side effect
1. A pharmaceutical composition comprising an aromatase inhibitor, a progestin and an oestrogen in the following ranges with reference to ethinyl oestradiol at 0.01mg having a value of 1:
the aromatase inhibitor is anastrozole in the range 10-1000; the progestin is levonorgestrel in the range 5-15; and the oestrogen is ethinyl oestradiol in the range 1-6.
|Indian Patent Application Number||3115/DELNP/2005|
|PG Journal Number||25/2010|
|Date of Filing||13-Jul-2005|
|Name of Patentee||ASTRAZENECA AB|
|Applicant Address||S-151 85 SODERTALJE, SWEDEN.|
|PCT International Classification Number||A61K 31/5685|
|PCT International Application Number||PCT/GB2004/000414|
|PCT International Filing date||2004-08-19|