Title of Invention

"A PROCESS FOR THE PREPRATION OF A PLANT GROWTH PROMOTORY BIOAGENT "

Abstract This invention relates to a process for the preparation of a plant growth promotory bioagent comprising 1-2% fungus Fusarium pallidoroseum isolated from roots of chilli plant, the fungus being grown on 98-99% twenty four hours pre-soaked sorghum grains comprising, chopping the root in 1 cm pieces, surface sterilizing with 0.12% HgCh, dissecting longitudinally and placing on potato dextrose agar media containing 18-22 ppm, preferably 20 ppm streptomycin sulphate, isolating the fungus and growing on synthetic media comprising dextrose, agar, potato extract and water and incubating in BOD inculcator at 27°C for four days, twenty four hours pre-soaked sorghum grains with 5-7% raw sugar, autoclaving at 15 lbs at 121°C for 50-70 minutes preferably 1 hour, cooling and then inoculating with six discs of 6 mm diameter from fully grown plate of fungal culture/flask, inoculating at 25-29°C preferably 27°C in BOD incubator for 7-10 days preferably 8 days, grinding the fully colonized grain to a powder and sieving though 2 mm sieve.
Full Text Field of Invention:
This invention relates to plant growth promotory bioagent and to a process for the preparation thereof.
Background of invention
The bioagents in the prior art utilized under organic agriculture or integrated crop management such as Rhizobium, Azospirillum, Azotobacter and arbuscular mycorrhizal fungi (AMF), contribute towards plant growth. The most widely used bioagent i.e., arbuscular mycorrhizae provide considerable help in increasing plant growth at vegetative/reproductive phase. However, because of the biotrophic nature of AMF the major constraint is its bulk inoculum production and therefore, it has limitation for inoculating in field at large scale.
Objects of invention:
An object of this invention is to propose a new bioagent enhancing vegetative/reproductive growth of atleast Chilli, Tomato, Wheat, Maize, Marigold, Okra, Moong, Brinjal etc.
Another object of this invention is to propose a bioagent which can grow on a synthetic medium.
A further object of this invention is to propose a bioagent which is more efficient than other biofertilizers.
Statement of Invention
According to this invention there is provided a plant growth promotory bioagent comprising of 98-99% sorghum grains and 1-2% of fungus such as herein described.
Further according to this invention there is provided a process for the preparation of bioagent comprising steps of:
Longitudinal dissection of small root pieces, its surface
sterilization in 0.12% HgC^ to obtain a fungus
isolation of fungus from the root piece plated on a synthetic
medium containing streptomycin sulfate @ 20ppm followed by
growth of the fungus on the synthetic medium to obtain disc of
fungus
inoculation of grains with disc of fungus and
incubation of inoculum and multiplication.
Brief Description of the accompanying drawings
Further objects and advantages of this invention will be more apparent from the ensuing description when read in conjunction with the accompanying drawings and wherein:
Please include Fig. 1 -3 and graph 1 -4 as provided earlier
Fig. 1 shows: Effect of bioagent on chilli
Fig. 2 shows: Effect of bioagent on tomato
Fig. 3 shows: Fungus growth on synthetic medium
Graph 1 shows: Fresh wt. shoot of chilli
Graph 2 shows: Dry wt. shoot of chilli
Graph 3 shows: Fresh wt. shoot of tomato
Graph 4 shows: Dry wt. shoot of tomato
Graph 5 shows: Effect of Bioagent on shoot length
Graph 6 shows: Effect of Bioagent on shoot weight
Graph 7 shows: Effect of Bioagent on Root weight
Description of Invention with reference to accompanying drawings:
According to the present invention, fungus is isolated from Chilli plant. The plant material namely root is chopped in for example 1 cm pieces and surface sterilized with HgCl2 solution (0.12%). The root pieces are dissected longitudinally and placed on
potato dextrose agar media containing streptomycin sulfate @ 18 - 22ppm preferably 20ppm. Thereafter, the fungus is isolated and grown on the synthetic medium comprising of dextrose, agar, extract of potato and water so as to obtain disc of fungus after incubation in BOD incubator at 27°C for four days (Fig. 3). The fungus/Bioagent is deposited in August, 2005 at CABI Bioscience UK, Bakeham, Lane Esham Surrey, TW20 9TY, UK which is identified as Fusarium pallidorosewn and accorded no. IMI No. 393490 dated 7.10.2005 having the following characteristic for identification.
It is common species in soil and plant debris in tropical and subtropical regions. Typically it exists as a saprobe, but it may act as a weak pathogen causing seedling blight, root rot, post-harvest, fruit rot, or leaf blight on a wide range of plants. The fungus does not form high levels of trichothecenes, but can produce other potentially toxic metabolites. This strain is as typical as it appears not to produce dry conidia in the serial mycelium, so its identification is tentative. Sequencing is recommended.
The fungus/bioagent is obtained from root of chilli plant and its Geographical origin is Pantnagar location (Udham Singh Nagar, Uttaranchal).
One hundred - hundred twenty five grams twenty four hours pre-soaked sorghum grains in water is filled in 250ml flask with 70 - 80 ml distilled water. Gur (raw
sugar) is added in these flasks @ 5-7% of soaked sorghum grains. The flasks are then autoclaved at 15 Ibs and 121 °C for 50-70 minutes preferably 1 hour, which after cooling, inoculated with six discs/flask (diameter 6mm) of fungal culture. The incubation is carried out at 25-29°C preferably 27°C in BOD incubator for 7-10 days preferably 8 days. These fully colonized grains are powdered in a grinder, and sieved through 2 mm sieve. The powder thus obtained having 2.5-3.Ox 10s conidia/g is used as inoculum @ 2.5-7% (w/w) in autoclaved soil which is filled in pots.
The chilli and tomato seeds were sown directly and also nursery seedlings were transplanted in these potting mixes. The growth responses in chilli and tomato are shown in Fig 1 and 2, respectively.
The plant growth i.e., shoot fresh & dry weight, root fresh and dry weight was observed. The fungal inoculation increased 150-300 and 150-200% shoot fresh and dry weight over control, respectively as shown in graphs 1-4. The growth responses particularly on wheat (Titicum aestivwri), maize (Zea mays), marigold (Tagetus minutd), okra (Abelmoschus esculentus), moong (Vigna radiata) and brinjal (Solanum melongend) were observed after 50 days of plant growth, which has been indicated hereinunder and in graphs 5-7.
wheat
Comparing to the control fresh weight of root, shoot and shoot length was increased by 14, 28.6 and 14%, respectively; however the dry weight of root and shoot was found to increase by 23.6 and 18.6% respectively.
Maize
An increase in fresh weight of root, shoot and shoot length was observed which was 169.6, 35.6 and 10.9%, respectively and dry weight of root and shoot was increased by 169.8 and 35.6% respectively compared to control.
Marigold
Compared with control, fresh weight of root, shoot and shoot length was increased by 137, 24.5 and 62.14%, respectively, however the dry weight of root and shoot increased by 155.3 and 25.2%, respectively.
Okra
An increase of 68, 19.3 and 27.6% was observed in fresh weight of root, shoot and shoot length respectively compared with control and dry weight of root and shoot was increased by 37.8 and 19.1%.
Moong
Fresh weight of root, shoot and shoot length was increased 420, 168.1 and 25.5%, respectively and the dry weight of root and shoot increased by 427.3 and 133.6% respectively, while comparing with control.
Brinjal
An increase in fresh weight of root, shoot and shoot length was recorded which was 17.7, 11.2 and 15.2%, respectively and dry weight of root and shoot was 18.4 and 11%, respectively compared with control.
It is to be noted that the present invention is susceptible to modifications, adaptations and changes by those skilled in the art. Such variant embodiments employing the concepts and features of this invention are intended to be within the scope of the present invention, which is further set forth under the following claims: -
















We Claim
1. A process for the preparation of a plant growth promotory bioagent comprising 1-2% fungus Fusarium pallidoroseum isolated from roots of chilli plant, the fungus being grown on 98-99% twenty four hours pre-soaked sorghum grains comprising:-
i) chopping the root in 1 cm pieces, surface sterilizing with 0.12% HgCl2, dissecting longitudinally and placing on potato dextrose agar media containing 18-22 ppm, preferably 20 ppm streptomycin sulphate
ii) isolating the fungus and growing on synthetic media
comprising dextrose, agar, potato extract and water and incubating in BOD inculcator at 27°C for four days.
iii) twenty four hours pre-soaked sorghum grains with 5-7% raw sugar, autoclaving at 15 lbs at 121°C for 50-70 minutes preferably 1 hour.
iv) cooling and then inoculating with six discs of 6 mm diameter from fully grown plate of fungal culture/flask.
v) inoculating at 25-29°C preferably 27°C in BOD incubator for 7-10 days preferably 8 days.
vi) grinding the fully colonized grain to a powder and sieving though 2 mm sieve.
2. A process for preparation of a plant growth promontory bioagent wherein the powder is having 2.5-3.0X105 conidia of Fusarium pallidoroseum/gm is used as inoculum @ 2.5-7% W/W in autoclaved soil.
3. A process for preparation of a plant growth promotory bioagent as described and illustrated herein.

Documents:

1002-del-2004-Abstract-(30-12-2009).pdf

1002-DEL-2005-Abstract-(13-07-2009).pdf

1002-del-2005-abstract.pdf

1002-DEL-2005-Claims-(08-12-2009).pdf

1002-DEL-2005-Claims-(13-07-2009).pdf

1002-del-2005-claims.pdf

1002-DEL-2005-Correspondence-Others-(13-07-2009).pdf

1002-DEL-2005-Correspondence-Others-(25-10-2010).pdf

1002-del-2005-correspondence-others.pdf

1002-del-2005-correspondence-po.pdf

1002-del-2005-description (complete).pdf

1002-del-2005-description (provisional).pdf

1002-DEL-2005-Drawings-(13-07-2009).pdf

1002-del-2005-drawings.pdf

1002-del-2005-Form-1-(30-12-2009).pdf

1002-del-2005-form-1.pdf

1002-DEL-2005-Form-15-(25-10-2010).pdf

1002-del-2005-form-18.pdf

1002-DEL-2005-Form-2-(13-07-2009).pdf

1002-del-2005-Form-2-(30-12-2009).pdf

1002-del-2005-form-2.pdf

1002-del-2005-form-5.pdf

1002-del-2005-GPA (13-7-2009).pdf

1802-DEL-2008-Form-5.pdf


Patent Number 237946
Indian Patent Application Number 1002/DEL/2005
PG Journal Number 4/2010
Publication Date 22-Jan-2010
Grant Date 14-Jan-2010
Date of Filing 21-Apr-2005
Name of Patentee G.B. PANT UNIVERSITY OF AGRICULTURE & TECHNOLOGY
Applicant Address PANT NAGAR-263 145, UTTARANCHAL, INDIA
Inventors:
# Inventor's Name Inventor's Address
1 DR. ANIL KUMAR SHARMA DEPTT OF BIOLOGICAL SCIENCES, COLLEGE OF BASIC SCIENCES & HUMANITIES, G.B. PANT UNIVERSITY, PANT NAGAR-263 145.INDIA.
2 DR. RASHMI SRIVASTAVA DEPTT OF BIOLOGICAL SCIENCES, COLLEGE OF BASIC SSCIENCES & HUMANITIES, G.B. PANT UNIVERSITY, PANT NAGAR-263 145.INDIA.
PCT International Classification Number C12P1/02
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA