Title of Invention


Abstract Pharmaceutical formulations comprising en epothilone in the form of an infusion concentrate or a lyophilised composition, and method of administration of an epothilone in suitable form fr parenteral administration.
Full Text

This invention is concerned with pharmaceutical formulations of epothilones, and in particular pharmaceutical formulations which are administrable parenterally, e.g. intravenously.
The epothilones represent a dass of microtubule stabilizing cytotoxic agents (see Gerth, K. et al. J. Antibiot 49,560-3 (1966); or Hoefle et al.. DE 41 38 042) of the fomiula I. Typical representatives include epothilone A wherein R is a hydrogen and epothilone B wherein R is a methyl group.

They are 16-member macrolides containing seven chiral centers and may also be characterized by various functionalities. For example, they may include other ring systems, such as an epoxide and/or a thiazole ring. They may have two free, derivatizable hydroxyl groups and the macrolide itself may comprise an ester linkage. The epothilones and their syntheses are described for example in published PCT application number WO 93/10121 and DE 41 38 042 A2, the contents of which are incorporated herein by reference. Typical epotiiilone derivatives and their syntheses are described in published PCT application number WO 97/19086 and WO 98/25929, the contents of which are incorporated herein by reference. Reference to the epothilones is preferably intended to mean epothilone A or epothilone B or their salts and derivatives or mixtures tiiereof as appropriate. Epothilone A or B may be used alone or they may be used as mixtures of A and B, preferably however they are used as solely A or solely B, most preferably solely B.
Cytotoxic agents are well known for the treatment of tumours. The anti-tumour activity of many of these compounds relies on the Inhibition of cell proliferation and consequent induction of apoptosis and cell death. The majority of cytotoxic agents exert their effects

through interference of DNA and/or RNA syntheses. However, for certain cytotoxic agents, e.g. members of the taxane family, e.g. paclitaxel, and the epothilones, their activity is reliant on their interference with microtubule dynamics. Microtubules are an important and attractive target for development of novel anti-cancer formulations.
However, little has been published on formulations suitable for epothilones. We have found that the 16-member macrollde system is particulariy labile to degradation. Moreover, the poor solubility of these compourKJs makes ft very difficult to form pharmaceutical formulations for parenteral administration. Poorly soluble compounds conventionally may be brought into solution by warrming the solvent during the dissolution process. However, given the high reactivity of these compounds they may be prone to degradation at elevated temperatures. Further, these highly reactive compounds may degrade over prolonged periods of storage as aqueous solutions. Concentrated solutions of the microtubule agent Taxol* which can be diluted In an aqueous medium prior to intravenous administration have been described. However, such solutions conventionally employ a surfactant such as Cremophor^ (polyethoxylated castor oil). It is well known that surfactants such as Cremophor^ can cause allergic reactions in patients.
Thus there is a need for commerdally acceptable pharmaceutical formulations suitable for epothilones, e.g. pharmaceutical formulations which allow for storage, e.g. in a refrigerator, e.g. at 2-8 °C.
We have now surprisingly found means to improve the solubility of an epothilone, e.g. epothllone A or epothilone B, and/or render them more rapidly soluble without the use of a surfactant, for example a surfactant having an HLB value of 10 or more, e.g. Cremophor^, and without adversely affecting their potency.
Accordingly, the invention provides in or>e of its aspects a pharmaceutical formulation comprising an epothilone, e.g. epothilone A or epothilone B, which hereinafter may be referred to as a formulation of the present invention.
In a preferred embodiment the invention provides a pharmaceutical formulation in the form of an infusion concentrate wihch comprises an epothilone and a pharmaceutically acceptable organic solvent, e.g. in the absence of a surfactant having an HLB value of 10

or above, e.g. Cremophor^. The infusion concentrate does not require the use of a surfactant to improve the solubility of an epothilone, e.g. epothilone A and B, and/or to render it more rapidly soluble. As stated above, surfactants such as a polyhydrogenated natural or hydrogenated castor oil, e.g. of an HLB value greater than 10, e.g. Cremophor^, may cause allergic reactions and they also can leach plasticisers from standard PVC containers, tubing and the like. Consequently, when they are employed one may be required to use special infusion apparatus, e.g. nitro-glycerine tubing and non-plasticised containers, such as glass, tubing and the like.
The aforementioned pharmac^iticalfy acceptable organic solvent may be chosen from any such organic solvent known In the art. Said solvent may be used individually or as combinations with other solvents. Preferably said dolvent is liquid at room temperature. Preferably the solvent is selected (i) from an alcohol with a carbon chain length of at least 2. e.g. Ca-Cs, e.g. C2 or C3 or C4, or (ii) from an N-alkylpyrrolidone. e.g. C1-C4, e.g. N-methylpyrrolidone. Typical examples of alcohols are, e.g. a water miscible alcohol, e.g. absolute ethanol, or glycerol. Other alcohols include glycols, e.g. any glycol obtainable from an oxide such as ethylene oxide, e.g. propylene glycol. Other examples are polyols, e.g. a polyalkylene glycol, e.g. poly{C2.3)alkylene glycol. A typical example is a polyethylene glycol, e.g. of a preferred molecular weight of 200-600 daltons. more preferably, 200-400 daltons, especially 300 daltons. Polyethylene glycols may be used in distilled form and may be characterised for example by one or more of the following features: (i) an ethylene oxide content of maximally 20 ppm, typteally less than 1 ppm, e.g. 0.1 to 0.5 ppm. (ii) a pH value between 4.0 to 7,0, and (iii) the absence of reducing substances and aldehydes {as determined by examination of the degree of coloration of the liquid to be examined in comparison with a reference solution comprising ferric chloride sailts (yellow solution) or cobalt chlorides salts (red solutions), according to experimental procedures described in European Pharmacopoeia, Third Edition, 1997, Council of Europe, Strasbourg, Chapter 2.2.2. Degree of coloration of liquids, p. 15 -17, incorporated herein by reference. One skilled in the art would reaRze that polyethylene glycols of various molecular weights may be used as long as tiiey are physiologically acceptable. The aforementioned solvents may of course contain residual water arising out of their production or being taken up from tiie atmosphere, e.g. up to saturation, e.g. up to 2 %, e.g. up to 0.5 %, e.g. typically less than 0-1 %. e.g. from 0.01 to 0.05 %. If desired, the pharmaceutically acceptable solvent may be mixed witti water f added watery, e.g about up to 45 % water, e.g. up to 30 %, e.g,

20 %. e.g. 5 %. Typical examples include ethanol/water mixtures, e.g 70% ethanol weight/volume (w/v), or polyethylene glycol/water mixtures, e.g. 90 % polyethylene glycol w/v.
The epothilones, for example epothilone A or epothilone B, may be present in an infusion concentrate in a concentration of 0.1 to 100 mg/ml, e.g. 1 to 100 mg/ml, more preferably 0.5 to 50 mg/ml, more preferably 0.5 to 10 mg/ml, most preferably 1 mg'mL
An epothilone, e.g. epothilone A or epothilone B, may be used incBviduaDy or as a mixture of epothilones, e.g. a mixture of ^x>tftfor>e A and B. Given ttie stror>ger anti-tumour activity of epothilone B it may be employed in a lower concentration than epothilone A in the formulation. When used alone it is preferable to employ a concentration of epothilone A of 0.1 to 100 mg/ml, e.g. 10 to 100 mg/ml, preferably 0.1 to 50 mg/ml, e.g. 20 to 50 mg/ml, and especially 1 mg/ml. Epothilone B if used alone, is preferably employed in a concentration of 0.1 to 50 mg/ml, e.g. 10 to 50 mg/ml, e.g. 1 to 50 mg/ml, and especially 1 mg/ml.
A pharmaceutical fonnulation of the present invention in the form of an infusion concentrate may be produced by working up, e.g. dissolving, an epothilone in a pharmaceutically acceptable solvent of the invention, optionally with other excipients. Preferably, no other excipients are present. If they are present they are present preferably in an amount of less than 5 %, e.g. less than 2 %, e.g. between 0.1 to 1.5 %.
Infusion concentrates of the present invention are convenientiy stored in suitable containers, e.g. vials, double-chamber vial systems, or ampoules. Typically the vials or ampoules are made from glass, e.g. boresilicate or soda-lime glass. The vials or ampoules may be of any volume conventional in the art, preferably they are of a size sufficient to accommodate 1 to 5 ml, more preferably 2 ml, of an infusion concentrate. The containers may accommodate preferably a stopper that can be pierced, e.g. a sterile njbber stopper, which may provide an appropriate hermetic seal with the container to allow for transfer of a liquid from or to the container.
The Infusion concentrates of the present invention may be stable for an exlerxJed period of time, e.g. up to 12 to 36, e.g. 24 months at temperatures of at least 2 to 8 ^'C, as indicated in standard stability tests, e.g. as described in the examples.

Furthermore, the infusion concentrates exhibit little evaporation, they may be produced using conventional equipment, e.g. no explosion-proof equipment is necessary, and they can tolerate rubber stoppers when stored in containers, e.g. without causing the stoppers to degrade.
Infusion concentrates may be diluted in a pharmaceutically acceptable oipanic solvent, e.g. an aqueous medium, suitable for intravenous administration to fomi an infusion solution, before the epothHone is admtnfetered parenterally. e.g. intravenously, to a patient It Is understood that, parenteral adrrmtstration includes administration by infusion or Injection.
Accordingly, the invention provides in another of its aspects an Infusion solution comprising in admixture an Infusion concentrate as hereinabove defined and a diluent selected from a pharmaceutically acceptable organic solvent, which is preferably an aqueous medium.
The pharmaceutically acceptable organic solvent used as a diluent may be any of those solvents or combinations of solvents used in the infusion concentrate. Preferably however it consists of water, i.e. water-for-injection. The Infusion solution preferably has the same or essentially the same osmotic pressure as body fluid. Accordingly, the diluent preferably contains an isotonic agent or agents which has the effect of rendering the osmotic pressure of the infusion solution the same or essentially the same as body fluid.
The isotonic agent or agents may t>e selected from any of those known in the art, e.g. mannitol, dextrose, glucose and sodium chbride. Preferably the isotonic agent is glucose or sodium chloride. The isotonic agent or agents may be used in amounts which impart to the infusion solution the same or essentially the same osmotic pressure as body fluid. The precise quantities needed can be determined by routine experimentation and may depend upon the composition of the infusion solution and the nature of the isotonic agent or agents. Selection of a particular isotonic agent or agents may be made havir>g regard to the properties of the epothilone, e.g. epothilone A or epothilone B. For example, when epothilone B is employed alone or in combination with epottiilone A, the use of certain isotonic agent or agents may cause tfie infusion solution to turn turbid. The turbidity may be attributed to the dissolution of the epothilone, e.g. epothilone B.

Surprisingly, we have found that if one employs glucose as the isotonic agent then the turbidity does not appear for long periods, e.g exceeding 24 hours, if at all.
The concentration of isotonic agent or agents in the aqueous medium will depend upon the nature of the particular isotonic agent or agents used, preferably the concentration is 5 % o less. When glucose is used It is preferably used in a concentration of from 1 to 5% w/v, more particularty 5% wV. When tf>e isotonic agent is sodium chloride it is preferably employed in amounts of up to 1 % w/v, in particular 0.9% w/v.
Infusion solutions according to the invention may comprise other excipients commonly employed in formulations to be administered intravenously. Excipients include antioxidants. Antioxidants may be employed to protect the epothilone, e.g. epothilone B, against oxidative degradation. Antioxidants may be chosen from any of those antioxidants known in the art and suitable for intravenous fonnulations. The amount of antioxidant may be determined by routine experimentation. As an alternative to the addition of an antioxidant, or in addition thereto, the antioxidant effect may be achieved by displacing oxygen (air) frorr contact with the infusion solution. This may be conveniently carried out by purging the container holding said infusion solution with an inert gas, e.g. nitrogen.
The amount of diluent used in admixture with the infusion concentrate in order to form an infusion solution may be chosen according to the desired concentration of epothilone, e.g. epothilone B, in the infusion solution. Preferably the infusion solution is prepared by mixing a vial or ampoule of infusion concentrate aforementioned with a diluent, e.g. a 5 % w/v glucose solution in water-for-injectlon in a suitable container, e.g. an infusion bag or bottle, making the volume up to between 50 ml and 1000 ml, e.g. 200 ml and 1000 ml or preferably 50 to 100 ml, with the diluent. The infusion solution so formed may be preferably used immediately or within a short time of t>eing formed, e.g. within 6 hours. Alternatively, the infusion concentrate and a predetermined amount of diluent, may be loaded each into separate chambers of a double-chamber vial system and only mixed immediately prior to intravenous administration to a patient
In an altemative embodiment a pharmaceutical formulation of the present invention may be in the form of a lyophiBsed conposltion comprising an epothiior>e, e.g. epotiiilone A or

epothilone B. Given the poor solubility of epothilone A and B a lyophillsate mass consisting only of an epothilone, e^g. epothilone A or epothilone B, may be very small such that it does not provide a iyophilised composition of suitable bulk to be handled conveniently, e.g. giving acceptable content uniformity, or even to the extent that it may even be difficult to detect visually. Accordingly, one may use excipients in a Iyophilised composition according to the invention which act to increase the scrfids content and therefore the bulk of the iyophilised composition. Suitable excipients may be any of those excipients which used alone or in combination will increase ttie IxiBc of the Iyophilised imposition without adversely interacting with the epothilone such as to destabilise the epothikDne or otherwise reduce its potency. Additionally, the exdfw^its r>eed to be suitable for use in pharmaceutical formulations, e.g. parenteral, e^ intravenous pharmaceutical formulations. Therefore when selecting an excipient or excipients consideration must be given not only to the nature of the Iyophilised composition but also to the nature of the final pharmaceutical form. Examples of suitable excipients include sodium or potassium phosphates, citric acid, tartaric acid, gelatin, lactose and other carbohydrates such as dextrose, mannitol and dextran and any of the cyclodextrins which are suitable for use intravenously, e.g. a beta-cyclodextrin, Typical beta-cyclodextrins include also beta-cyclodextrin derivatives, e.g. alkyl- or allyl-derivatives, or hydroxyalkyl-derivatives, which are for example formed by a condensation reaction of beta-cyclodextrin with an oxide, e.g. propyleneoxide. in a more preferred embodiment the beta-cyclodextrin derivative may be hydroxypropi^-beta-cyclodextrin. Preferably the hydroxypropyl-beta-cyclodextrin may be any of those mentioned by Roger A. Rajewski et al in the Journal of Pharmaceutical Sciences, Vol. 85, No. 11, November 1996. pages 1142 through 1169, which article is incorporated herein by reference.
Through judicious selection of excipients the applk:ant has found that a suitably bulky Iyophilised composition comprising an epothilone, e.g. epothilone A or epothilone B, may be formed which exhibits improved solubility characteristics of an epothilone, e.g. epothilone A or epothilone B, or renders the epothilone more rapidly soluble but which does not adversely affect the potency of the epothilone.
The excipients or mixtures thereof may contribute to 50 to 99.9 % of the total soFids content of the Iyophilised composition, more preferably 90 to 99 %, e.g. 95 % of total solids of said composition.

The epothilone may contribute 100% to the total solids content of the lyophilised composition although preferably It may contribute to 0.1 to 10 %. more preferably 0.1 to 1.5 %. e.g. 1 -2% of total solids.
To the extent that the epothilone and cyclodextrin or mannitoi do not provide 100% of the total solids content of the lyophiRsed composition, the balance of soFids may be provided by any excipients commonly used in tfie field of lyophllisates whrdi are to be reconstituted for phamnaceutical use, e.g any of the otiier excipients referred to hereinabove.
Lyophilised compositions according to the invention may be formed from solutions (hereinafter referred to as "original solutions") containing an epothilone, e.g. epothilone A or epothilone B, and suitable excipients as defined hereinabove. Suitable solvents for such original solutions are either water alone or aqueous based solvents containing pharmaceutically acceptable, water miscible organic solvents, e.g. alcohols, more parlicularty ethanol or polyethylene glycol,
Original solutions may contain from 0.01% to 0.5% (w/v) of epothilone, e.g. epothilone A or epothilone B.
Original solutions may be prepared by dissolving the epothilone, e.g. epothilone A or epothilone B, and excipients in a suitable solvent and thereafter filtering the solution through a filter, e.g. a sterile 0.22 micron filter. The original solution thus formed may be filled into vials of suitable volume, preferably having a volume of 30 ml and a fill volume of 4,2 ml.
In yet another aspect the present invention provides a method of producing a lyophilised composition which comprises the steps of (i) mi)dng an epofrillone, e.g. epothilone A or epothilone B, with a pharmaceuticaffy acceptable exciplent, e.g. mannitoi or a cyclodextrin, e.g. a hydroxypropyl-beta-cydodextrin in a suitable solvent to form an original solution, and (ii) dehydrating the original solution.
Lyophilisation may be carried out according to known tedmtques. In a prefemed process the aforementioned filled vials may be frozen in a lyophiBsafion chamt>er for approximately 3

hours at a temperature below the eutectic point, preferably approximately - 40 °C. Thereafter the lyophilisation chamber may be evacuated to about 0.1 to 0.2 miiiitorr. The temperature of the chamber may then be increased to effect sublimation of the frozen liquids. Preferably the temperature is increased to about 0 °C and this temperature may be maintained for a period of 8 to 15 hours to effect lyophilisation.
The lyophilised composition may be used in tiie production of parenteral formulations and so the lyophilisation process is preferably carried out under sterile conditions, for example using aseptic production process^ or by irradiation. Aseptic formation of solutions containing pharmaceutlcally ati&ve compounds, the aseptic filling of vials and lyophilisation processes under aseptic conditions are well known to the skilled addressee.
The moisture content of the lyophilised composition thus formed may be 3% or less of the total weight of the lyophilised composition. Optionally however, a humidification step may be employed subsequent to lyophilisation wherein sterile water vapour may be introduced into the lyophilisation chamber at atmospheric pressure or at a reduced pressure as aforementioned. Of course, if the humidification step is canied out under reduced pressure, the pressure may vary with the introduction of the water vapour, and pressure changes can be monitored and pressure adjusted if necessary using techniques well known in the art. The humidification step may be completed with a time period of from 4 to 8 hours depending on whether it is carried out at atmospheric pressure or reduced pressure.
Lyophilised compositions obtained using a humidification step are hereinafter referred to as hydrated lyophilisates. Said hydrated lyophilisates may contain from 0.1 to 5 % by weight of water.
Lyophilised compositions according to the present invention may be provided in single dosage container forms. The single dosage container forms may be of any suitable size. By "suitable size" is meant an appropriate size having regard to the volume of solution which will be needed to reconstitute \he lyophiFesed composition. Any suitable containers may be used to provide these dosage forms. By "suitable" is meant any container which may be used in aseptic filling procedures are! whteh is capable of maintaining a sterile environment and which is unreactive to the fyofrftilised composition. Preferred containers may t>e formed of glass, e.g. Type I glass and may have means to receive a stopper, e.g. a sterile rubber

stopper which may cooperate with the walls of the container to provide a hermitic seal. Preferred stoppers also may allow entry to the contents of the container for the purpose of introduction of a solvent, e.g. water for Injection, for the lyophilised composition.
The lyophilised composition according to the invention may be storage stable for up to 24 to 36 months at a temperature of 2 to 30 °C. Lyophilised compositions stored for these periods display no signs of degradation ar>d the solubility characteristics iBmain unaffected.
When it is desired to provide a paraiterBl, e.g. intraveneous, form di an epothilone, the lyophilised composition may be re-consffiuted, preferably just before administration.
Re-constitution may involve dlssolving the lyophilised composition in water or some other pharmaceutically acceptable solvent as hereinabove described, for example physiological saline, an aqueous solution of a pharmaceutically acceptable alcohol, e.g. ethanol, propylene glycol, a polyethylene glycol, e.g. polyethylene glycol 300, and the like, or some other sterile injectable under aseptic conditions. The single dosage container fonn may be filled with an appropriate quantity of solvent having regard to the desired concentration of epothilone, e.g. epothilone A or epothilone B, required for parenteral administration. Such a reconstituted lyophilised composition may be preferably used immediately or within a short time of being formed, e.g. within 6 hours.
A pharmaceutical formulation of the present invention in suitable form for parenteral, e.g. intravenous administration, e.g. an infusion solution prepared by diluting an infusion concentrate or a reconstituted lyophilised composition (hereinafter refen^d to as diluted formulations of the present invention), may be filled in containers diosen from any conventional container which is non-reactive to said pharmaceutical formulations. Glass containers made from those glass types aforementioned are suitable although it is prefenBd to use plastics containers, e.g. plastic infusion bags.
Plastics containers may be principally ttiose composed of thermoplastic polymers. Plastics materials may additionally comprise additives, e.g. plastidsers, fillers, antiowdants, antistatics and otiier additives conventional in tiie art

Plastics suitable for diluted formulations of the present invention should be resistant to elevated temperatures required for thermal sterilisation. Preferred plastics infusion bags are those made from PVC plastics materials known in the art.
A wide range of container sizes may be employed. When selecting a container size, consideration may be paid to \he solubUity of the epothilone in the particular solvent and the ease of handling and. if appropriate, storage of the container. It \s pref enred to use containers which can accommodate between about 200 to 1000 mi, e-g. 250 to 1000 ml. of a diluted fonriulation of the present invention.
A diluted formulation of the present invention may be preferably sterile. This may be readily accomplished, e.g. by irradiation or by filtration of said diluted fonnulation through sterile filtration membranes. Aseptic formation of any composition in liquid form, the aseptic filling vials and/or combining of liquids for parenteral use with a suitable diluent under aseptic conditions are well known to the skilled addressee.
A diluted formulation of the present invention is useful for treatment and prevention of malignant proliferative disorders, for example the indications and conditions disclosed in WO 93/10121 and DE 41 38 042 A2, the contents of which are incorporated herein by reference. More specifically, they may be useful for the treatment of a tumour disease, e.g. a melanoma, ovarian cancer, pancreas cancer, neuroblastoma, head and neck cancer, bladder cancer, renal, brain, gastric or preferably a colorectal, prostate, breast, lung (especially non-small cell lung) or epithelial, especially epidermoid, e.g. cervical, cancer. Moreover, a diluted foimulatlon of the present invenfion Is benefidal in treating conditions for which and in the same manner as Paditaxel* is used. For certain tumours epothllones offer enhanced beneficial effects compared with Paclitaxef*. For certain tumours, e.g. certain types of lung tumours, e.g. A549 lung epothilone B offers enhanced beneficial effects compared with Paditaxel*.
Generally, a diluted formulatfon of the present invention may be administered in an amount v^ich is therapeutically effective against a proHferative disease tiiat can be treated by administration of an epothitone, e.9. epothilone A and/or epothflor>e B, espedaify epothitone B. Such proliferative diseases indude any proliferative disease as mentioned above, espedaify a tumour cfesease, the re^x>nse to a tiierapeuticaHy effectfve amount preferably

manifesting itself in a diminished proliferation, e.g. diminished tumour growth or even (more preferably) tumor regression or (most preferably) tumour disappearance. The exact amount and the duration of administration may depend upon the nature of the epothilone, e.g. epothilone A, epothilone B or a mixture of both, the particular type of malignantly proliferating cells characteristic of the particular tumour, the seriousness of the condition, the rate of administration, as weH as the patienf s health and response to treatment
Also, a pharmaceutical fomiulation of tiie present inventton in suitable form for parenteral administration, e.g. an infusion sofeition prepared by diluting an ffrfusion concentrate or a reconstituted lyophilised composfBon, may be combined with other tumour treatments known to a skilled person, e.g. radiation, or administered as part of a combination therapy comprising at least one other chemotherapeutic agent. The administration of a combination of active agents may be simultaneous or consecutive, with either one of the active agents being administered first. The dosage of the active agents of a combination treatment may depend on effectiveness and site of action of each active agent as well as synergistic effects between the agents used for combination therapy.
Other chemotherapeutic agents may include especially any chemotherapeutic agent that is or can be used in the treatment of tumor diseases, such as chemotherapeutics derived from the following classes:
(A) alkylating agents, preferably cross-linking chemotherapeutics, preferably bis-alkylating agents,
(B) antitumour antibiotics, preferably doxorubicin (Adriamycin®, Rubex®);
(C) antimetabolites;
(D) plant alkaloids;
(E) honmonal agents and antagonists;
(F) biological response modifiers, preferably lymphokines or interferons;
(G) inhibitors of protein tyrosine kinases and/or serine/threonine kinases;
(H) antisense oligonucleotides or oTigonucleotkJe derivatives; or
(I) miscellaneous agents or agents with other or unknown mechanism of action, preferably of the Taxane class, espedally Taxotere® or most especiaify paditaxel (Taxol®).

A diluted formulation of the present invention may, therefore, be useful as a single anti¬cancer fomiulations or as part of a combination regimen for the treatment of various tumours.
The utility of all diluted formulations of the present invention may be observed in standard clinical trials in, for example, known indications of epothilone dosages giving equivalent blood levels of epothilone; for examj^ using dosages in the range of about 0.1 to 6 mg/m^ of epothilone for weeldy treatment and about 0.3 to 18 mg^m^ of epothilone for three-weeiciy treatment for a 75 kilogram marr^nai, e.g. an adult hum^i of 1.73 m^ and in standard animal models. For examfrfe, the anfr-tumor effect of single dose regimens are investigated in a model of human ovarian cancer SKOV3 as well as a U373 glioma model.
The increased bioavailability of an epothilone administered in the form of a diluted formulation of the present invention, may be observed in standard animal tests and in ' clinical trials, e.g. as described above. Naturally, the exact amounts of epothilone and of the pharmaceutical formulation to be administered may depend on a number of factors, e.g. the condition to be treated, the exact epothilone, the desired duration of treatment and the rate of administration of epothilone. For example, the amount of epothilone required and the administration rate thereof may be detemiined on the basis of known in vivo and in vitro techniques, for example as described above, determining how long a particular epothilone concentration in the blood plasma remains at an acceptable level for a therapeutic effect.
Diluted formulations of the present invention may be conveniently administered Intravenously in a dosage of from about 0.1 to 100 mg/m^ e.g. 0.2 to 100 mg/m^ epothilone A and from about 0.1 to 50 mg/m^, e.g. 0.2 to 50 mg/m^ of epothlJor>e B. Preferably, for weekly treatment the dose is between 0.1 and 6 mg/m^, preferably 0.1 and 5, more preferably 0.1 and 3, even more preferably 0.1 and 1.7 mg/m^ most preferably 0.1 and 1 mg/m^; for three-weekly treatment the dose is between 0.3 and 18 mg/m^ preferably 0.3 and 15, more preferably 0.3 and 12, even more preferabiy 0.3 and 7.5 mg/m^ most preferably 1.0 and 3.0 mg/m^ This dosis is preferably administered to a human by intravenous administration during 2 to 180 min, preferabiy 2 to 120 min, more preferat)ly during 5 to 30 min, most preferaWy during 10 to 30 min, e.g. durir>g 30 min.

Preferably the concentration and dosage strength may be such to achieve an effective dose level of about 0.5 to 15 mg/day, more preferably 1 to 10 mg/day, more preferably 2 to 8 mg/day. The dose received by intravenous administration and the blood concentration may be determined accurately on the basis of known in vivo and in vitro techniques.
In yet another aspect the invention pn^vides a method of administering an epothiione to a subject in need of epothiione treatment whicti comprises administering parenterally a diluted formulation of the present invention to a subject in need of such treatmerrt. More specifically, such a method of adrrHnistering an epothiione comprises (a) diluting a pharmaceutical formulation acconSng to the invention, e.g. in the form of an infusion concentrate or a lyophilised composition, with an aqueous medium, to form a solution suitable for parenteral, e.g. intravenous, administration, and (b) administering such a solution to the subject.
In yet another aspect the invention provides use of an epothiione in the manufacture of a medicament suitable for parenteral administration.
The invention is illustrated by way of the following examples which are not intended to limit the scope of the present invention. All percentages are by weight/weight unless otherwise specified. Any components of the pharmaceutical formulations may further be described in Fiedler, H. P. "Lexikon der Hilfsstoffe fur Pharmazie, Kosmetik und angrenzende Gebiete", Editio Cantor, D-7960 Aulendorf, 4th revised and expanded edition (1996), the contents of which are hereby incorporated by reference.

Example 1
Epothilone (15 mg of B or 50 mg of A) is dissolved in 98-100 % propylene glycol (1.0 ml). The solution is sterile filtered through a 0.22 microns pore size filter and charged to 1 ml ampoules. The filled ampoules are used for storage and shipment. The filled ampoules are stable for a period of at least 12 morrths at a temperature of 2 to 8 X>. Prior to intravenous administration, the contents of an ampoule are added to 250 to 1^000 m! of a 5 % glucose solution in water-for-rnjection. The intravenous solution thus fomied is stable for a period of 8 hours at room temperature.
Examples 2 to 7
The experiment of Example 1 is repeated using the absolute and aqueous ethanol solvent systems and various polyethylene glycol solvent systems (Table 1).
Table 1:

The infusion solutions obtained from Examples 2 to 7 are all stable for a period of 8 hours at room temperature.
Example 8
Solubilities in various solvent syst^iis are summarized in Table 2. If not indicated othenvise all solubility data refer to T = 22 tiJ.

The solubility of epothilone B In water at neutral pH is about 160 mg/l, and significantly higher solubility is achieved in PEG/water, propyleneglycol/water, or EtOH/water mixtures. In comparison, previously reported aqueous solubility of epothilone A is 940 mg/l and 700 mg/i for mixtures of epothilones A and B.
Example 9
The stability of aqueous versus nonaqueous polyethyleneglycol infusion concentrates comprising epothilone B at different concentratkxi and various temperatures is determined. Typically, a known amount of ^x)thilor>e B Is dissolved in 1.0 ml of each of the various solvent systems and each soKifion is sterile filtered and charged to 1 rrt white-glass vjals with grey rubber stoppers and grey l%>-off caps. Table 3 describes tf>e amount of

degradation product formed over a period of up to seven months. The stability is analyzed by determining formation of degradation products in each of the infusion concentrates as a function of time and temperature. Each sample is analyzed by HPLC. the sample is prepared by diluting the concentrate with an aqueous medium. The stability of all infusion concentrates after 3 months at 2 to 8 "^C is comparable. At higher temperatures, e.g. 25 "^C, nonaqueous solvent systems comprising PEG eyhibH generally higher stability than aqueous solvent systems comprising PEG.

Example 10
An aqueous solution Is prepared by dissolving epothilone B (5.0 mg) and mannitol (1500 mg) in water for injection to make up a 30 ml solution. The solution is passed through a sterile 0.22 micron pore size membrane filter before aseptically filling the solution into a glass vial and thereafter aseptically fitting a sterile stopper to the vial in readiness for the drying process. The filled vial Is then positioned in a lyophillsation chamber and cooled to a temperature of about -40 °C, The lyophffisator condenser is cooled to about -60 ""C and the chamber is evacuated to about 0.1 mllftorr. The chamber temperature is set to about 20 **C to start the drying process. After about 20 hours of drying the chamt)er pressure has Increased to about 0.2 millitorr and the drying process is de^Ded compiete. The pressure of the chamber is increased to atmospheric pressure by aseptically introducing sterile air or nitrogen into the chamt)er. Thereafter the stopper Is aseptically seated onto the vial to

provide a hermitic sterile seal. The sealed vial provides a single dose container of epothilone B which is reconstituted shortly before administration with 25 ml of water for injection. The dosage is administered intravenously.
The lyophilised product possesses the desired characteristics required of the lyophilised compositions according to the invention.
Examples 11 to 14
The methodology of Example 10 is carried out \n respect of the tabulated components set forth hereinbelow to form lyopWnsed products (Table 4).

The lyophilised products formed according to Examples 11 to 14 possess the desired characteristics required of the lyophilised compositions according to the invention.

1. A lyophilised composition comprising (i) an epothilone and (ii) mannitol or a cyclodextrin.
2. The lyophilised composition comprising an epothilone and hydroxypropyl-beta-cyclodextrin.

3. The lyophilised composition according to claim 1 or 2 wherein the epothilone represents 0.1 to 1.5 % of the total solids.
4. The lyophilised composition according to claim I or 2 wherein the epothilone represents 0.1 to 1.5 % of the total solids and a cyclodextrin represents 90 to 99% of the total solids.
5. A reconstituted lyophilised composition comprising a pharmaceutical
formulation as described in anyone of claims 1 to 4 in a pharmaceutically acceptable
6. A method of administering an epothilone which comprises:
(a) reconstituting the lyophilised composition according to anyone of claims 1 to 4 with an aqueous medium to form a solution and
(b) administering the solution intravenously to the subject.

7. The method of administering an epothilone to a subject in need of epothilone treatment which comprises administering parenterally a reconstituted lyophilised composition according to claim 5 to a subject in need of such treatment. .
8. Use of a lyophilised composition according to anyone of claims 1 to 4 in the manufacture of a medicament suitable for parenteral administration.


1058-che-2006 complete specification as granted.pdf

1058-CHE-2006 AMANDED CLAIMS 19-10-2009.pdf



1058-CHE-2006 OTHER DOCUMENT 19-10-2009.pdf

1058-CHE-2006 OTHER PATENT DOCUMENT 19-10-2009.pdf

1058-CHE-2006 CORRESPONDENCE OTHERS 23-10-2009.pdf



1058-CHE-2006 FORM 18.pdf

1058-CHE-2006 FORM-3 23-10-2009.pdf




1058-che-2006-form 1.pdf

1058-che-2006-form 26.pdf

1058-che-2006-form 3.pdf

1058-che-2006-form 5.pdf

Patent Number 237655
Indian Patent Application Number 1058/CHE/2006
PG Journal Number 2/2010
Publication Date 08-Jan-2010
Grant Date 01-Jan-2010
Date of Filing 21-Jun-2006
Name of Patentee NOVARTIS AG
Applicant Address Schwarzwaldallee 215, CH-4058 Basel, Switzerland
# Inventor's Name Inventor's Address
1 VAN HOOGEVEST, Peter, Breitenstrasse 3, CH-4416 Bubendorf, Switzerland
PCT International Classification Number A61K31/425
PCT International Application Number N/A
PCT International Filing date 1999-02-03
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 9802451.6 1998-02-05 U.K.
2 9813646.8 1998-06-24 U.K.