Title of Invention

A SYNERGISTIC FUNGICIDAL COMPOSITION

Abstract

The invention herein relates to a synergistic fungicidal composition and to a process for the preparation thereof. In particular, the wettable powder formulation of this invention comprises of bioeffective amounts of dithiocarbamate fungicide and Hexaconazole fungicides. The wettable powder formulation of the invention is prepared by mixing appropiate quantities of dithicarbamate fungicide and Hexaconazole with suitable adjuvants using a unique process of blending and pulverizing.

Full Text

COMPLETE AFTER PROVISIONAL LEFT ON 4/5/06 FORM - 2 THE PATENTS ACT, 1970 (39 of 1970) & THE PATENTS RULES, 2003 COMPLETE Specification (See section 10 and rule 13) A SYNERGISTIC FUNGICIDAL COMPOSITION INDOFIL CHEMICALS C (a division of MODIPON LTD.) an Indian Company of Nirlon House, Dr. Annie Besant Road, Mumbai 400 025, Maharashtra, India, THE FOLLOWING SPECIFICATION PARTICULARLY DESCRIBES THE INVENTION AND THE MANNER IN WHICH IT IS TO BE PERFORMED. 4 MAY 2006 TITLE A synergistic fungicidal composition FIELD OF INVENTION The present invention relates to a synergistic fungicidal composition. This invention envisages a fungicidal composition consisting of synergistic amount of Dithiocarbamate and Triazole group of fungicides. INTRODUCTION Most crop and ornamental plants are subject to attack by several fungi. The diversity of these organisms and their potential for inciting serious disorders in combination or singly has resulted in the need for broad-spectrum disease control. Although there are available numerous chemical compounds (fungicides), which aid in preventing diseases of plants, each of these has practical deficiencies, which restrict its use. The inability of any one fungicide to control all of the potentially serious fungi on one-host makes the use of combinations or mixtures of fungicides a part of normal control practice. ZINEB IUPAC : Zinc ethylene bis (dithiocarbamate) polymeric CAS : 12122-67-7 MOLECULAR WEIGHT : 275.8 MOLECULAR FORMULA : C4H6N2S4Zn Water solubility : 10 mg / L Form : pale yellow powder Zineb is foliar fungicide with protective action. Uses: control of downy mildews in vines, hops, lettuce, onions, spinach, brassicas, rapeseed, tobacco, and ornamentals; rusts in currants, berry fruit, plums, vegetables, and ornamentals. Red fire disease on vines, potato and tomato blights, leaf spot diseases on currants, berry fruit, olives, and celery; Anthracnose on beans, vines and citrus fruit and Scab on apples and pears, Shot-hole on stone fruit. 2 HEXACONAZOLE IUPAC : (RS )-2-(2,4-Dichlorophenyl)-l-(lH-l,2,4-triazol-l-yl) hexan-2-ol CAS : 79983-71-4 MOLECULAR WEIGHT : 314.2 MOLECULAR FORMULA : C14H17CI2N30 Water solubility : 0.017 g / L ( 20 °C) Form : White crystalline solid Hexaconazole is a Systemic fungicide with protective and curative action Uses: Control of many fungi on apples, vines, coffee, peanuts. Also used on bananas, cucurbits, peppers and other crops. BACKGROUND OF THE INVENTION US 4474805 discloses a synergistic mixture of fungicides consisting of N-2-6-dimethyl phenyl)-N-(l-methoxycarbonyl-ethyl)-phenyl-acetamide and a dithiocarbamate selected from the group consisting of Zineb and Mancozeb. US patent 6172094 discloses a synergistic mixture of Carbamate and a dithiocarbamate selected from the group consisting of manganese ethylene (dithiocarbamate) ( Zinc complex ) ,(mancozeb) manganese ethylene bis(dithiocarbamate), (Maneb) zinc ammoniate ethylene bis (dithiocarbamate) and zinc ethylene bis(dithiocarbamate) . (Zineb) where in the weight ratio of the dithiocarbamate to the carbamate is 200:1 to 0.1:1. US patent 4937261 discloses a synergistic mixture of propyl 3-(dimethylamino ) propyl carbamate or an acid addition salt thereof, and a compound selected from the group consisting of Propineb , (Maneb) , metiram and zinc (Zineb) where in the weight ratio of the Component A to component B is from about 1.0.1 to 3. US patent 406025 discloses a synergistic mixture of methyl 2- benzimidazole carbamate with manganous or zinc salts of 3 ethylenebisdithiocarbmic acid i.e maneb or Zineb ) the preferred ratio is from 1:2 to 1:6. Formulations of Maneb and Zineb are well known, thus wettable powders and dusts are disclosed in US pat Nos 2,504404, 2665285, 2719822, 2974156, and 3085042, 3173832. Above prior art does not mention the combination of dithiocarbamate with an Azole fungicide. Fungicidal compositions comprising Zineb as active ingredient are known and have been found to be effective against fungal diseases such as leaf spot, fruit rot , scab, rust etc in economically important crops such as grape, tea, mango, cotton, rice etc Zineb 75% wp is available commercially as wettable powder. Fungicidal compositions containing Triazoles as active ingredient are known and have been found be effective against fungal diseases such as scab, blast, sheath blight, powdery mildew, tikka , leaf spot, fruit spot, etc in economically important crops such as apple, grape , groundnut , peas, mango etc. Triazole fungicides such as Hexaconazole, Tebuconazole, and Propiconazole are available commercially in the form of wettable powder (WP), water dispersible granule (WDG), Emulsifiable concentrate (EC) and Suspension concentrate (SC) concentrations. Considering the importance of effective control of a broad spectrum of fungal diseases such as scab, blast, blight, mildew, tikka, leaf spot , fruit spot etc in economically important crops such as mango, tobacco, coconut, turmeric, tea potato, etc the need for a fungicidal composition which is stable, ready to use and less expensive was felt. Further it has been observed that whenever two individual fungicides such as Zineb and Triazole fungicide are used in combination, due to the presence of both types of fungicides, the combination gives a better control 4 of fungal diseases than when they are used individually due the synergistic action. OBJECT OF THE INVENTION The object of the invention is to provide a synergistic fungicidal composition. Yet another object of the invention is to provide a process for the preparation of a synergistic fungicidal composition. Accordingly, the main objective of the present invention is to provide an improved, stable and ready to use fungicidal composition of dithiocarbamate and a triazole fungicide which is effective against a wide spectrum of fungi such as scab, blasts, spots, rots, blights, mildews, ruts, etc. and which does not give rise to any fungal resistance. Another objective of the present invention is to provide an improved stable and ready to use fungicidal composition, the bio-efficacy of which is superior to the mixture of the individual formulations prepared. Yet another objective of the present invention is to provide an improved stable and ready to use fungicidal composition of dithiocarbamate and a triazole fungicide which is non-phytotoxic and results in high yield and superior quality of various economically important crops such as potato, tea etc. Still another objective of the present invention is to provide an improved stable and ready to use fungicidal composition of dithiocarbamate and a triazole fungicide which is in the wettable powder form along with suitable wetting, dispersing and suspending agents resulting in a stable formulation when used in the field. Another objective of the present invention is to provide an improved stable and ready to use fungicidal composition of dithiocarbamate and a triazole 5 fungicide where in the active ingredients used are in lower concentration as compared to the known fungicide formulations containing individual ingredients currently available commercially viz Zineb 75% wp, Hexaconazole 5EC (Trade names of commercially available formulations Contaf 5EC, Sitara 5EC). Still Another objective of the present invention is to provide an improved stable and ready to use fungicidal composition of dithiocarbamate and a triazole fungicide which results in a fungicidal composition that is effective against a wide spectrum of fungi such as scab, blasts, spots, blights, mildews, rusts and does not given rise to fungal resistance. Yet another objective of the present invention is to provide an improved stable and ready to use fungicidal composition of dithiocarbamate and a triazole fungicide. Which is non-phytotoxic, the composition producing higher yield due to its better control of the diseases resulting in lower incidence of fungal diseases there by giving about 5 to 25% increased yield. Still another objective of the present invention is to provide an improved stable and ready to use fungicidal composition of dithiocarbamate and a triazole fungicide which overcomes the problems faced by the farmers in the field of physically mixing the individual, commercially available formulations at the desired field. SUMMARY OF THE INVENTION According to this invention, therefore there is provided a synergistic fungicidal composition, comprising a) a bioeffective amount of dithiocarbamate; b) a bioeffective amount of Hexaconazole; c) at least one stabilizer having mass of about 0.5% to 7% of the total mass of the composition; d) at least one wetting agent having mass of about 1 to 5 % of the total mass of the composition; e) at least one suspending agents having mass of about 2 to 13% of the total mass of the composition; 6 f) at least one carrier having mass of about 3 to 8% of the total mass of the composition. Typically, the mass of dithiocarbamate ranges from about 60 to 70 % of the total mass of the composition. Typically, the mass of Hexaconazole ranges from about 2 to 6 % of the total mass of the composition. In accordance with one embodiment of the invention, the stabilizer is at least one compound selected from a group of compounds consisting of sodium metabisulphite (SMBS), Hexamethylene tetra amine (HMT), glycol and paraformadehyde. Typically, the mass of the stabilizer preferably ranges from about 1 to 5% of the composition. In accordance with another embodiment of the invention, the wetting agent is at least one compound selected from a group of compounds consisting of alcohol alkoxylate, fatty alcohol ethoxylates, alkyl naphthalene sulfonate condensate, sodium lauryl sulphates, and sodium dodecyl benzene sulfonate. Typically, the mass of the wetting agent preferably ranges from about 1 to 3 % of the composition. In accordance with another embodiment of the invention, the suspending agent is at least one compound selected from a group of compounds consisting of sodium ligno sulfonate, calcium ligno sulfonate and sodium salt of alkyl naphthalene sulfonate condensate. Typically, the mass of the suspending agent preferably ranges from about 2 to 10% of the composition. 7 In accordance with another embodiment of the invention, the carrier is at least one compound selected from a group of compounds consisting of clay, precipitated silica, and talc. Typically, the mass of the carrier preferably ranges from about 4 to 7 % of the composition. In accordance with another aspect of the invention, there is provided a process for preparing a synergistic fungicidal composition comprising the following steps a) mixing dithiocarbamate with Hexaconazole and other agents selected from carriers, wetting agents, suspending agents to get a mixture; b) the mixture pulverized in a pulverizer (ribbon blender, kitchen mixer, hammer mill pulverizer, fluid energy air jet mill, a pin disk mill) to achieve 2 to 5 micron particle size; c) the pulverized mixture is post blended to get product of desired composition. DESCRIPTION OF THE FIGURES Figure 1 is a drawing showing how typically a synergistic fungicidal composition comprising of dithiocarbamate and Hexaconazole is formulated using fluid energy air jet mill lab model equipment comprising of five stages. Required quantity of adjuvant, stabilizer and Hexaconazole technical is charged to blender in stage-1 of Pre-Mixing. Blended mixture is directed from blender to jet mill via pump where it is milled to reduce the particle Size (Range 2 to 5 microns) in stage-2 of pulverizing. Pulverized mixture is transferred to Blend tank 2 where the required quantity of dithiocarbamate technical is added in stage-3 of Post Blending to obtain dithiocarbamate and Hexaconazole blend. In stage -5 of Sieving and Packing the wettable powder product having particle size in the range of 2 to 5 microns is analyzed. Figure 2 is a drawing showing how typically a synergistic fungicidal composition comprising of dithiocarbamate and Hexaconazole is formulated 8 using fluid energy air jet mill lab model equipment comprising of five stages. Required quantity of adjuvant, stabilizer, carrier, dithiocarbamate technical and Hexaconazole technical is charged to blender in stage-1 of Pre-Mixing. Blended mixture is directed from blender to jet mill via pump where it is milled to reduce the particle Size (Range 2 to 5 microns) in stage-2 of pulverizing. Pulverized mixture is transferred to Blend tank 2 where it is blended in stage-3 of Post Blending to obtain dithiocarbamate and Hexaconazole blend. In stage-5 of Sieving and Packing the wettable powder product having particle size in the range of 2 to 5 microns is analyzed. DETAILED DESCRIPTION OF THE INVENTION This invention relates to a fungicidal composition with synergistic activity. The composition contains, as essential ingredients, two components that mutually affect each other when used together and display a biological activity that is greater than the sum of the activities when used alone, an affect, which is designated as synergism. Systemic product needs right partner to prevent resistance development and the combination product of Zineb + Hexaconazole can solve this problem. Besides being broad-spectrum, opportunity exists in solving disease problems. In the course of our studies of co-formulations of fungicide we have found, surprisingly that the co formulation of Zineb with Hexaconazole results in a synergistic mixture there of which is remarkably more effective than the individual compounds at the considered doses. The required dithiocarbamate is prepared by adding an aqueous solution of ZnCI2 or ZincSulphate to an aqueous solution of disodiumethylene bisdithiocarbamate. To the resulting slurry required quantity of stabilizer and dispersing agent is added to get the dithiocarbamate, which is used, in the experiment. The Azole Fungicide is commercially purchased from GSP. 9 Accordingly, the present invention provides synergistic mixtures of fungicide (A) contact dithiocarbamate in particular and (B) selected non-systemic fungicides belonging to the following group or class Azole. The composition of the invention shows a surprising fungicidal activity that exceeds the sum of the activities of the individual components when used alone. However, the weight ratio of component A and B depends on the sensitivity and resistance of the plants, the time of application, the climatic conditions and the soil conditions. The present invention provides the preferred method of initially mixing one of the active ingredient (Azole preferably Hexaconazole) with suspending agent / stabilizer in ribbon blender. This mixture is then ground in a Jet mill. To this milled mixture a specified quantity of dithiocarbamate is added to achieve the preferred ratio. Mixture obtained is then mixed again in ribbon blender to yield the desired composition. In the mixture of the invention, the range of proportions can be from 0.3: 6 to 1: 10 of Azole: dithiocarbamate. This invention particularly relates to a synergistic mixture of Dithiocarbamate and Azole in wettable powder form. Modification can be made to the process which include direct blending of Zineb wettable powder and Hexaconazole technical with necessary dispersing agents and fillers. Accordingly the present invention provides an improved, stable and ready to use fungicidal composition which is effective against a wide spectrum of fungi such as scab, blasts blights, etc in a wettable powder form which comprises an dithiocarbamate fungicide equivalent to 60 to 70% by weight as Zineb and Triazole fungicide (Hexaconazole) in an amount of 2 to 6% by weight of the composition and other formulation adjuvant and axillaries selected from amongst carriers, wetting agents, dispersing agents, 10 suspending agent or a mixtures thereof in an amount of 3 to 15% by weight of the composition In a preferred embodiment of the present invention the dithiocarbamate fungicide used in the composition is Zineb. The amount of the Dithiocarbamate used in the composition may be in such a concentration so as to give 60 to 70% Zineb. The triazole fungicide, which is used in the composition, is Hexaconazole. In a preferred embodiment of the invention the triazole used in the composition is Hexaconazole in an amount of 2 to 6% by weight. The carriers employed in the composition of the present invention may be combination of clay, precipitated silica, and talc. More preferably the carriers used may be China clay. The carriers employed may be present in an amount in the range of 3 to 8% by weight of the composition, more preferably in the range of 4 to 7 % by weight of the composition. The stabilizers employed in the composition of the present invention may be sodium metabisulphite (SMBS), Hexamethylene tetra amine (HMT), glycol, paraformadehyde or may be combination of HMT and SMBS. The stabilizers employed may be present in an amount in the range of 0.5% to 7% by weight of the composition, more preferably in the range of 1 to 5% by weight of the composition. The wetting agent employed in the wettable powder formulation may be non-ionic surfactants belonging to the class of alcohol alkoxylate and fatty alcohol ethoxylates. Wetting agent may also be an anionic surfactant belonging to alkyl naphthalene sulfonate condensate or sodium lauryl 11 sulphate or sodium dodecyl benzene sulfonate, preferably a blend of anionic and non-ionic surfactant belonging to class of naphthalene sulfonate and fatty alcohol ethoxylates. The wetting agents employed in the wettable powder formulation may be present in an amount of 1 to 5 % by weight, more preferably 1 to 3 % by weight of the composition. The suspending agents employed in the wettable powder formulation may be sodium ligno sulfonate or calcium ligno sulfonate or sodium salt of alkyl naphthalene sulfonate condensate, more preferably sodium salt of alkyl naphthalene sulfonate condensate. The suspending agents employed in the wettable powder formulation may be present in a amount of 2 to 13% by weight, preferably in 2 to 10% by weight of the composition. According to another embodiment of the present invention there is provided a process for the preparation of an improved, stable and ready to use fungicidal composition which is effective against a broad spectrum of fungi such as scab, blast, blight, mildew etc in economically important crops such as apple, rice, potato etc which comprises mixing thoroughly an dithiocarbamate fungicide in an amount equivalent to 60 to 70% by weight of Zineb with a Hexaconazole fungicide in an amount of 2 to 6% by weight and other agents selected from carriers, wetting agents, suspending agents and or a mixtures there of in an amount of 3 to 15% by weight of the composition and pulverizing the mixture in a pulverizer. The mixing may be effected using a ribbon blender or kitchen mixer. The pulverizing may be done to achieve 2 to 5 micron particle size. The pulverizer used may be a hammer mill pulverizer or fluid energy air jet mill or a pin disk mill. This is further post blended to get product of desired composition. 12 According to another preferred embodiment of the present invention there is provided a process for the preparation of an improved, stable and ready to use fungicidal composition which is effective against a broad spectrum of fungi such as scab, blast, blight, mildew etc in economically important crops such as apple, rice, potato etc which comprises mixing thoroughly Triazole (Hexaconazole) active ingredient in an amount of 2 to 6% by weight and other agents selected from carriers, wetting agent, suspending agent, stabilizer, or a mixtures thereof in ribbon blender. This is then ground in a jet mill. To this milled mixture a specified quantity of dithiocarbamate (Zineb) is added and again mixed in ribbon blender or suitable blender to yield the desired fungicidal composition. EXAMPLES The details of the present invention are given in the examples provided below which are given only by way of illustration and therefore should not be constructed to limit the scope of the invention List of Adjuvant Surfactant A : Sodium ligno sulphonate ( Borresperse NA) Surfactant B: Sulfonated naphthalene condensate, sodium salt (Lomar D from cognis) Surfactant C: Sodium alkyl naphthalene sulphonate (Morwet IP from Akzonobel) Surfactant D: Blend of Sodium alkyl naphthalene sulphonate and anionic surfactant (Morwet EFW from Akzonobel) Surfactant E: Sulfonated aromatic polymer sodium salt ( Morwet D-425 from Akzonobel) Surfactant G: Blend of anionic surfactants belonging to class of naphthalene sulphonate ( Jeemol DW from Jeevan chemicals ltd) Surfactant I: Sodium salt of dibutyl naphthalene sulphonate (Jeemol BX from Jeevan chemicals ltd ) Surfactant H: Blend of alcohol alkoxylate and fatty alcohol ethoxylates (Jeemol DML From Jeevan Chemicals ltd) Surfactant F: sodium lauryl sulphate 13 Stabilizer 1: Hexamethylene tetra mine Stabilizer 2: Sodium metabisulphite Carrier 1: China clay Example no 1 : To Hexaconazole Technical was added Surfactant C (Morwet IP) and Stabilizer 2 (sodium metabisulphite) in a blender. This mixture was blended in blender for two hrs. The Mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. The pulverization is repeated until the particle size of the formulation is less than 5 microns .To the pulverized material is added desired quantity of Zineb and blended in a ribbon blender or kitchen mixer to get a homogeneous wettable powder formulation. Zineb used is wettable powder commercially available as Z-78 (Brand name). Final composition on dry basis Percent on dry basis Zineb wettable powder (75%) 93.00 Hexaconazole technical (92%) 4.8 Surfactant C 0.7 Stabilizer 2 1.5 Example no 2: To Hexaconazole Technical was added Surfactant I (Jeemol BX), Surfactant G (Jeemol DW), Stabilizer 2 (sodium metabisulphite) in a ribbon blender and blended in blender for 2 hours. The mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. To the pulverized material is added required quantity of Zineb and further blended in a blender for half an hour to get a homogeneous wettable powder formulation. 14 Final composition on dry basis Percent on dry basis Zineb wettable powder (75%) 92.00 Hexaconazole technical (92%) 5.00 Surfactant I 1.50 Surfactant G 0.50 Stabilizer 2 1.0 Example no 3: To Hexaconazole Technical was added Surfactant G(Jeemol DW), Surfactant H (Jeemol DML), Surfactant I ( Jeemol BX), Stabilizer 2 (sodium metabisulphite) in a ribbon blender and blended in blender for 2 hours. The mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. To the pulverized material is added required quantity of Zineb and further blended in a blender for half and hour to get a homogeneous wettable powder formulation. Final composition on dry basis Percent on dry basis Zineb wettable powder (75%) 92.00 Hexaconazole technical (92%) 5.00 Surfactant I 0.67 Surfactant G 1.00 Stabilizer 2 1.00 Surfactant H 0.33 Example no 4: To Hexaconazole Technical was added Surfactant C(Morwet IP), Surfactant E (Morwet D-425), Stabilizer 2 (sodium metabisulphite) in a ribbon blender and blended in blender for 2 hours. The mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. To the pulverized material is added required quantity of Zineb 15 and further blended in a blender for half an hour to get a homogeneous wettable powder formulation. Final composition on dry basis Percent on dry basis Zineb wettable powder (75%) 92.00 Hexaconazole technical (92%) 5.00 Surfactant C 1.50 Surfactant E 0.50 Stabilizer 2 1.00 Example no 5: To Hexaconazole Technical was added Surfactant G (Jeemol DW), Surfactant I ( Jeemol BX ), Stabilizer 1 (Hexamethylene tetra amine ) in a ribbon blender and blended in blender for 2 hours . The mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. To the pulverized material is added required quantity of Zineb and further blended in a blender for half an hour to get a homogeneous wettable powder formulation. Final composition on dry basis Percent on dry basis Zineb wettable powder (75%) 92.00 Hexaconazole technical (92%) 6.00 Surfactant G 0.20 Surfactant I 0.80 Stabilizer 1 1.00 16 Analysis of samples formulated (Zero day analysis) Analysis of samples formulated (After accelerated storage study of 14 days at 54° ± 2°C ) There was no appreciable change in any of the parameters for example no 1 to 4. In example no 5 Parameter change was seen after accelerated storage study. 17 Example no 6: To Zineb Technical was added, Hexaconazole Technical. To this was added Surfactant I(Jeemol BX) ,Surfactant G ( Jeemol DW ), Surfactant H (Jeemol DML), Surfactant A (sodium Ligno sulphonate), Carrier 1 (china clay), Stabilizer 2 (sodium metabisulphite) in a ribbon blender and blended in blender for 2 hours. The mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. The pulverization is repeated until the particle size of the formulation is less than 5 microns. The Pulverized material is further blended in a blender for half an hour to give a homogeneous wettable powder meeting various quality parameters Final composition on dry basis Percent on dry basis Zineb Technical 80.00 Hexaconazole technical 5.00 Surfactant G 3.00 Surfactant I 0.50 Surfactant H 1.00 Surfactant A 4.00 Stabilizer 2 1.00 Carrier 1 5.50 Example no 7 and 8 : To Zineb Technical was added Hexaconazole Technical. To this was added Surfactant C (Morwet IP), Surfactant E (Morwet D-425), Surfactant A (sodium Ligno sulphonate), Carrier 1 (china clay), Stabilizer 2 (sodium metabisulphite) in a ribbon blender and blended in blender for 2 hours. The mixture was pulverized using a pulverizer maintaining a feed rate of 10 to 20 g/min. The pulverization is repeated until the particle size of the formulation is less than 5 microns. The Pulverized material is further blended in a blender for half an hour to give a homogeneous wettable powder meeting various quality parameters 18 Final composition on dry basis Percent on dry basis Percent on dry basis Example no 7 Example no 8 Zineb Technical 80.00 80.00 Hexaconazole technical 5.00 5.00 Surfactant C 2.00 1.50 Surfactant E 2.00 3.00 Surfactant A 3.50 4.00 Stabilizer 2 1.00 1.00 Carrier 1 6.50 5.50 Analysis of samples formulated (Zero day analysis) 19 Samples formulated showed improved bioefficacy than individual components when used alone. Bio-efficacy of Zineb + Hexaconazole 72% WP against disease complex in Paddy Trial No. 1 Test product : Zineb 68% + Hexaconazole 4% WP Target Pest ■ Sheath Blight, Brown spot, Glumes Discoloration Crop • Paddy Paddy is a major crop grown in several regions across the country. However, onslaught of several diseases viz., Brown Spot, Sheath Blight, Leaf Blast and Panicle Discoloration are a major constraint in successful Paddy cultivation. Field experiments were therefore conducted in a crop field to evaluate the efficacy of new combo fungicide Zineb + Hexaconazole as per details given below: Crop Variety ; Swarna Number of treatments • Seven Number of replications • Three Plot Size 7x3m(21m2) Water volume used ■ 500 L/ Ha METHODOLOGY A field trial was laid out in Randomized Block Design in crop field in the Kharif season of 2005. Paddy var. "Swarna" was transplanted on 8th August, 2005 and harvested on 25th November, 2005 20 In all three sprays of fungicides were given starting from 15th September, 2005. The following treatments were evaluated: Observations on Brown Spot as well as on leaf spot were recorded on a 0-4 scale. Sheath blight was evaluated on the following scale 0 - No infection 1 - 1-10% vertical spread of disease 3 3 - 11-25% vertical spread 5 - 26-50% vertical spread 7 - 50-75% vertical spread 9 - >75% vertical spread The PDI were recorded by using following formula: PDI = Sum of all Disease Ratings_ - _x 100 Total Number of Ratings x Maximum Disease Grade 21 The data on Brown spot severity showed that all the treatments were significantly superior over control (31.83%). All doses of Zineb + Hexaconazole were effective to check brown spot severity in comparison to other treatments. Zineb + Hexaconazole @ 2.5 and 3.0g/L recorded 13.00% and l2.66% PDI respectively. The data on Sheath Blight showed that Zineb + Hexaconazole @ 2.5 & 3.0 g/ litre was significantly superior over control (40.56%) and statistically similar to Hexaconazole @ 2.0 ml/ litre (1.11% - 1.48%). However, combinations of Zineb + Hexaconazole contain lesser active ingredient of Hexaconazole as compared to single treatment. The results establish that in combination lower doses of Hexaconazole was effective against Sheath blight The data for panicles with glume discoloration per unit area showed that among at all doses tested, combination of Zineb + Hexaconazole was most effective and superior over either Hexaconazole (8.6%) and Zineb (9.5%) and control(14.9%). Highest yield was recorded in Zineb + Hexaconazole @ 3g/L (38.0 q/ha) which was on par with Zineb + Hexaconazole @ 2.0 & 2.5 g/ litre (36.7 & 36.8 q/ ha). 22 Trial No. 2 Variety 1010 Number of treatments Eight Number of replications ■ Three Plot Size 10 x 10 m Date of Sowing , 20. 12 .2005 Date of Transplanting ■ 12.01.2006 Date of Sprays 18.02.2006, 04.03.2006 Date of harvest 14.04.2006 Water volume used 500 L/ Ha In all two sprays of fungicides were given starting on 15th September, 2005. The following treatments were evaluated: The results indicate that minimum Brown spot was observed with Zineb + Hexaconazole @ 2.5 g/ lire (10.32%) which was also comparable to the tank mix application and as well as individual application of Zineb 23 (10.35%). However, in combination treatments active ingredient content of Zineb is lower as compared to individual treatments. Similarly leaf blast was minimum in Zineb containing treatments were found effective to suppress Leaf Blast of which Zineb + Hexaconazole @ 2.5 g/ litre was most effective. Zineb + Hexaconazole @ 2".5 g/ litre also recorded the maximum yield (43.18 / ha) which was also statistically superior over all other treatments Bio-efficacy of Zineb + Hexaconazole against paddy diseases Treatments Dose (g/lt) Brown Spot Leaf Blast Sheath Blight Yield (Q /ha) Zineb +Hexaconazole 72%WP 1.5 18.78 4.00 18.46 42.70 Zineb +Hexaconazole 72%WP 2.0 14.65 3.50 10.33 43.02 Zineb +Hexaconazole 72%WP 2.5 10.32 2.88 6.00 43.18 Hexaconazole 5 EC 2.0 19.48 7.02 2.33 40.80 * Zineb- 75 WP 2.5 10.35 3.63 25.45 38.85 Sitara+Z-78 (Tank Mix) * 2.0+2. 5 9.66 4.00 6.35 42.95 Control Water 28.32 11.20 38.25 36.50 CD at 5 % 2.57 1.5 1.97 2.3 * Sitara: Hexaconazole 5% EC; IZ 78 = Zineb 75% WP Conclusion: Zineb + Hexaconazole at 2.5 - 3.0 g/L was most effective against Sheath blight (Rhizoctonia oryzae), Brown Spot (Helminthosporium oryzae) and complex fungi which causes glume discoloration on the panicles. 24 We Claim: 1. A synergistic fungicidal composition, comprising a) a bioeffective amount of dithiocarbamate; b) a bioeffective amount of Hexaconazole; c) at least one stabilizer having mass of about 0.5% to 7% of the total mass of the composition; d) at least one wetting agent having mass of about 1 to 5 % of the total mass of the composition; e) at least one suspending agents having mass of about 2 to 13% of the total mass of the composition; f) at least one carrier having mass of about 3 to 8% of the total mass of the composition. 2. A synergistic fungicidal composition as claimed in claim 1, wherein the mass of dithiocarbamate ranges from about 60 to 70 % of the total mass of the composition. 3. A synergistic fungicidal composition as claimed in claim 1, wherein the mass of Hexaconazole ranges from about 2 to 6 % of the total mass of the composition. 4. A synergistic fungicidal composition as claimed in claim 1, wherein the stabilizer is at least one compound selected from a group of compounds consisting of sodium metabisulphite (SMBS), Hexamethylene tetra amine (HMT), glycol and paraformadehyde. 5. A synergistic fungicidal composition as claimed in claim 4, wherein the mass of the stabilizer preferably ranges from about 1 to 5% of the composition. 6. A synergistic fungicidal composition as claimed in claim 1, wherein the wetting agent is at least one compound selected from a group of compounds consisting of alcohol alkoxylate, fatty alcohol ethoxylates, 25 alkyl naphthalene sulfonate condensate, sodium lauryl sulphates, and sodium dodecyl benzene sulfonate. 7. A synergistic fungicidal composition as claimed in claim 6, wherein the mass of the wetting agent preferably ranges from about 1 to 3 % of the composition. 8. A synergistic fungicidal composition as claimed in claim 1, wherein the suspending agent is at least one compound selected from a group of compounds consisting of sodium ligno sulfonate, calcium ligno sulfonate and sodium salt of alkyl naphthalene sulfonate condensate. 9. A synergistic fungicidal composition as claimed in claim 8, wherein the mass of the suspending agent preferably ranges from about 2 to 10% of the composition. 10. A synergistic fungicidal composition as claimed in claim 1, wherein the carrier is at least one compound selected from a group of compounds consisting of clay, precipitated silica, and talc. 11. A synergistic fungicidal composition as claimed in claim 10, wherein the mass of the carrier preferably ranges from about 4 to 7 % of the composition. 12. A process for preparing a synergistic fungicidal composition as claimed in any one of the preceding claims comprising the following steps a) mixing dithiocarbamate with Hexaconazole and other agents selected from carriers, wetting agents, suspending agents to get a mixture; b) the mixture pulverized in a pulverizer (ribbon blender/ kitchen mixer/ hammer mill pulverizer/ fluid energy air jet mill/ a pin disk mill) to achieve 2 to 5 micron particle size; c) the pulverized mixture is post blended to get product of desired composition. 26 13. A composition and a process for preparing the composition substantially as herein described and illustrated with reference to the examples. 27

Documents:

555-mum-2005-abstract (provisional).doc

555-mum-2005-abstract (provisional).pdf

555-MUM-2005-ABSTRACT(4-5-2006).pdf

555-mum-2005-abstract(granted)-(10-7-2009).pdf

555-MUM-2005-CANCELLED PAGES(11-5-2009).pdf

555-mum-2005-claims (complete).doc

555-MUM-2005-CLAIMS(11-5-2009).pdf

555-mum-2005-claims(4-5-2006).pdf

555-mum-2005-claims(granted)-(10-7-2009).pdf

555-MUM-2005-CORRESPONDENCE(11-5-2009).pdf

555-mum-2005-correspondence(23-7-2007).pdf

555-mum-2005-correspondence(ipo)-(11-9-2009).pdf

555-mum-2005-deed of assignment(11-5-2009).pdf

555-mum-2005-description (complete).pdf

555-mum-2005-description (provisional).pdf

555-MUM-2005-DESCRIPTION(COMPLETE)-(11-5-2009).pdf

555-mum-2005-description(complete)-(4-5-2006).pdf

555-mum-2005-description(granted)-(10-7-2009).pdf

555-mum-2005-description(provisional)-(5-5-2005).pdf

555-mum-2005-drawing(granted)-(10-7-2009).pdf

555-MUM-2005-FORM 1(5-5-2005).pdf

555-mum-2005-form 13(16-4-2008).pdf

555-mum-2005-form 18(13-12-2010).pdf

555-mum-2005-form 18(24-7-2007).pdf

555-mum-2005-form 2(11-5-2009).pdf

555-mum-2005-form 2(4-5-2006).pdf

555-mum-2005-form 2(granted)-(10-7-2009).pdf

555-mum-2005-form 2(provisional)-(5-5-2005).pdf

555-MUM-2005-FORM 2(TITLE PAGE)-(11-5-2009).pdf

555-mum-2005-form 2(title page)-(4-5-2006).pdf

555-mum-2005-form 2(title page)-(granted)-(10-7-2009).pdf

555-mum-2005-form 2(title page)-(provisional)-(5-5-2005).pdf

555-MUM-2005-FORM 26(13-12-2010).pdf

555-mum-2005-form 6(13-12-2010).pdf

555-mum-2005-form-2 (complete).doc

555-mum-2005-form-2 (complete).pdf

555-mum-2005-form-2 (provisional).doc

555-mum-2005-form-2 (provisional).pdf

555-mum-2005-form-26.pdf

555-mum-2005-form-3.pdf

555-mum-2005-form-5.pdf

555-MUM-2005-OTHER DOCUMENT(13-12-2010).pdf

555-mum-2005-specification(amended)-(11-5-2009).pdf