Title of Invention	AN IN-VITRO PROCESS FOR THE DETECTION OF THE ACTIVITY OF GAMMA-SECRETASE
Abstract	This invention relates to an in-vitro process for the detection of the activity of y-secretase wherein A. a transgene is used which encodes a fusion protein comprising: a) a first nucleotide sequence which codes for a protein which contains the amino acid sequence GAIIGLMVGGVIA TVIVITL VML (SEQ ill. NO.1) wherein said protein can be cleaved within said amino acid sequence by y-secretase present in a cell, whereby a first partial protein, which contains the amino acid sequence GAIIGLMVGGVV (SEQ ill NO.2), and a second partial protein, which contains the amino acid sequence VIVITLVML (SEQ ill NO.3) are formed, b) at the 5' end of the first nucleotide sequence, ,a second nucleotide sequence which codes for a signal peptide, c) a promoter and, d) further coding nucleotide sequences which code for a protein which is expressed as a fusion protein with the first partial protein and the second partial protein and which is used for the detection of the second partial protein, and, if appropriate, further non-coding nucleotide sequences; B. this transgene is incorporated into a human cell or a non-human eukaryotic cell and the fusion protein is expressed; c. the fusion protein is cleaved within the amino acid sequence SEQ ill NO.1 by y-secretase present in the cell, whereby a first partial protein and the second partial protein are formed and D. the second partial protein is detected, characterized in that with the exception of SEQ ill No.1 said fusion protein does not contain one or more peptide motifs acting as a signal for endo- or exocytosis and/or protease cleavage site.

Title of Invention

AN IN-VITRO PROCESS FOR THE DETECTION OF THE ACTIVITY OF GAMMA-SECRETASE

Abstract

This invention relates to an in-vitro process for the detection of the activity of y-secretase wherein A. a transgene is used which encodes a fusion protein comprising: a) a first nucleotide sequence which codes for a protein which contains the amino acid sequence GAIIGLMVGGVIA TVIVITL VML (SEQ ill. NO.1) wherein said protein can be cleaved within said amino acid sequence by y-secretase present in a cell, whereby a first partial protein, which contains the amino acid sequence GAIIGLMVGGVV (SEQ ill NO.2), and a second partial protein, which contains the amino acid sequence VIVITLVML (SEQ ill NO.3) are formed, b) at the 5' end of the first nucleotide sequence, ,a second nucleotide sequence which codes for a signal peptide, c) a promoter and, d) further coding nucleotide sequences which code for a protein which is expressed as a fusion protein with the first partial protein and the second partial protein and which is used for the detection of the second partial protein, and, if appropriate, further non-coding nucleotide sequences; B. this transgene is incorporated into a human cell or a non-human eukaryotic cell and the fusion protein is expressed; c. the fusion protein is cleaved within the amino acid sequence SEQ ill NO.1 by y-secretase present in the cell, whereby a first partial protein and the second partial protein are formed and D. the second partial protein is detected, characterized in that with the exception of SEQ ill No.1 said fusion protein does not contain one or more peptide motifs acting as a signal for endo- or exocytosis and/or protease cleavage site.

Full Text

Documents:

3139-CHENP-2005 ABSTRACT.pdf

3139-CHENP-2005 CLAIMS.pdf

3139-CHENP-2005 CORRESPONDENCE OTHERS.pdf

3139-CHENP-2005 CORRESPONDENCE PO.pdf

3139-CHENP-2005 DESCRIPTION (COMPLETE).pdf

3139-CHENP-2005 DRAWINGS.pdf

3139-CHENP-2005 FORM-1.pdf

3139-CHENP-2005 FORM-18.pdf

3139-CHENP-2005 FORM-3.pdf

3139-CHENP-2005 FORM-5.pdf

3139-CHENP-2005 PCT.pdf

3139-CHENP-2005 PETITIONS.pdf

3139-CHENP-2005 POWER OF ATTORNEY.pdf

« Previous Patent

Next Patent »

Patent Number

229718

Indian Patent Application Number

3139/CHENP/2005

PG Journal Number

13/2009

Publication Date

27-Mar-2009

Grant Date

19-Feb-2009

Date of Filing

24-Nov-2005

Name of Patentee

SANOFI-AVENTIS DEUTSCHLAND GmbH

Applicant Address

Bruningstrasse 50, D-65929 Frankfurt am Main,

Inventors:

#	Inventor's Name	Inventor's Address
1	HOPPE, Edmund	Gartenstrasse 12, 82152 Krailling,
2	ROTHBLATT, Jonathan	Samannstrasse 102, 81249 Munchen,
3	LEBERER, Ekkehard	Fichtenstrasse 82 B, 82110 Germering,
4	MERCKEN, Luc	18bis, rue Jules Joffrin, F-94100 Saint Maur,
5	DREISLER, Sylvie	51, Avenue Carnot, F-91370 Verrieres,
6	PERAUS, Gisela	Agnes-Bernauer-Strasse 15, 80687 Munchen,

PCT International Classification Number

C07K 14/47

PCT International Application Number

PCT/EP2004/004550

PCT International Filing date

2004-04-29

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	03011807.9	2003-05-26	EUROPEAN UNION