Title of Invention	PLEUROMUTILIN DERIVATIVES AS ANTIMICROBIALS
Abstract	Abstract (3123/CHKNP/2004) "PLEUROMUTlLIN DERIVATIVES AS ANTIMICROBIALS" Compounds of formula wherein the residues have various meanings and their use as pharmaceutically active compounds.

Full Text

PLEUROMUTILIN DERIVATIVES AS ANTIMICROBIALS PI lilim The present invention relates to pleuromutilins having pharmaceutical, e.g. antimicrobial, activity. In one aspect the present invention provides a compound of formula wherein Rt and FV are hydrogen or deuterium, R2, R3 and R4 are hydrogen or deuterium, R5 is the residue of an amino acid, e.g. a valyl or histidinyl residue, X is S or N-ALK, In another aspect the present invention provides a compound of formula I, wherein R,, FV, R2, R3 and R, are hydrogen, R5 is the residue of an amino acid, e.g. a valyl or histidinyl residue, XisS, In another aspect the present invention provides a compound of formula I selected from the group consisting of - 14-0-[4-hydroxy-N-valy!-piperidin-3-yl]-sulfanylacetylmutilin, such as 14~0-[4-hydroxy-N- {R)-valy1-piperidin-3-yl]-sulfanylacetylmutilin, e.g. in the form of a hydrochloride, - 14-0-[3-hydroxy-N-valyl-piperidin-4-yl]-sulfanylacetylmutilin, such as 14-0-[3-hydroxy-N- (R)-valyl-piperidin-4-yl]-sulfanylacetyimutilin, e.g. in the form of a hydrochloride, - 14-0-[3-hydroxy-N-histidinyl-piperidin-4-yl]-sulfanylacetylmutilin, such as 14-0-[3-hydroxy-N-(R)-histidinyl-piperidin-4-yl]-sulfanylacetylmutilin, e.g. in the form of a dihydrochloride, - 14-0-[3-hydroxy-N-valyl-piperidin-4-yl]-methylaminoacetylmutilin, such as 14-0-[3-hydroxy-N-(R)-valyl-piperidin-4-yl]-methylaminoacetylmutilin, e.g. in the form of a dihydrochloride, - 14-0-[4-hydroxy-N-valy!-piperidin-3-yl]-methylaminoacetylmutilin, such as 14-0-[4-hydroxy- N-(R)-valyl-piperidin-3-yl]-methylaminoacetylmutilin, e.g. in the form of a dihydrochloride, - 14-0-[N-valyl)-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin, such as 14-0-[N-(R)- valyl-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin, and - 14-0-[N-valyl)-1,4,5,6-tetrahydropyridin-4-yl]-sulfanylacetylmuti!in, such as 14-0-[N-(R)- valyl-1,4,5,6-tetrahydropyridin-4-yl]-sulfanylacetylmutilin. Compounds of formula 1 may be prepared by splitting off a protecting group from compounds of formula 1 wherein functional groups, e.g. amino groups, are protected. Such compounds may be thus useful as intermediates in the production of a compound of formula I, or may be pharmaceutically active. R? is tert.butoxycarbonyl or the residue of an amino acid wherein the amino group is protected by tert.butoxycarbonyl, e.g. N-BOC protected valyl or histidinyl, X is S or N-ALK, Protecting group include protecting groups which may be, e.g. selectively, removed, if desired, and include protecting groups which are conventional in chemistry, e.g. (pleuro)mutilin chemistry, preferably BOC, e.g. which BOC can be removed e.g. by treatment with etheric HCI. In another aspect the present invention provides a compound of formula II selected from the group consisting of - 14-0-[N-BOC-4-hydroxy-piperidin-3-yl]-sulfanylacetylmutilin, - 14-0-[N-BOC-3-hydroxy-piperidin-4-yl]-sulfanylacetylmutilin, - 14-0-[4-hydroxy-N-BOC-piperidin-3-yl]-methylaminoacetylmutilin, - 14-0-[3-hydroxy-N-BOC-piperidin-4-y!]-methylaminoacetylmutilin, - 14-0-[N-BOC-1,4,5,6-tetrahydropyridin-4-ylJ-sulfany!acetylmutilin, such as 14-0-[N-BOC-1,4,5,6-tetrahydropyridin-4(R*)-yl]-sulfanylacetylmutilin and 14-0-[N-BOC-1,4,5,6-tetrahydropyridin-4(S*)-yl]-sulfanylacetylmutilin, - 14-0-[4-hydroxy-N-(N-BOC-valyl)-piperidin-3-yl]-sulfanylacetylmutilin, such as 14-0-[4-hydroxy-N-(N-BOC-(R)-valyl)-piperidin-3-yl]-sulfanylacetylmutilin, e.g. in the form of a hydrochloride, - 14-0-[3-hydroxy-N-(N-BOC-valyl)-piperidin-4-yl]-sulfanylacetylmutilin, such as 14-0-[3-hydroxy-N-(N-BOC-(R)-valyl)-piperidin-4-yl]-sulfanylacetylmutilin, e.g. in the form of a hydrochloride, 14-0-[4-acetoxy-N-{N-BOC-valyl)-piperidin-3-yl]-sulfanylacetylmutilin, such as 14-Q-[4-acetoxy-N-(N-BOC-(R)-valyl)-piperidin-3-yl]-sulfanylacetylmutilin, e.g. in the form of a hydrochloride, ■ 14-0-[3-acefoxy-N-(N-BOC-valyl)-piperidin-4-yl]-suffanylacetyimuti(in, such as 14-0-[3-acetoxy-N-{N-BOC-(R)-valyl)-piperidin-4-y!]-sulfanylacetylmutilin, e.g. in the form of a hydrochloride, ■ 14-0-[3-hydroxy-N-{N-BOC-histidinyl)-piperidin-4-ylj-su]fanylacetylmutilin, such as 14-0-[3-hydroxy-N-(N-BOC-(R)-histidinyl-piperidin-4-yl]-suifanyiacetylmutilin, e.g. in the form of a dihydrochloride. - 14-0-[3-hydroxy-N-(N-BOC)-valyl-piperidin-4-yl]-methylaminoacetylmutilin, such as 14-0-[3-hydroxy-N-(N-BOC)-(R)-valyl-piperidin-4-yl]-methylaminoacetylmutilin, e.g. in the form of a dihydrochloride, - 14-0-[4-hydroxy-N-(N-BOC)-valyl-piperidin-3-yl]-methylaminoacety!mutilin, such as 14-0-[4-hydroxy-N-(N-BOC)-(R)-valyl-piperidin-3-yl]-methylaminoacetylmutilin, e.g. in the form of a dihydrochloride, • 14-0-[N-(N-BOC-valyl)-1,4,5,6-tetrahydropyridin-4-yl]-sulfanylacetylmutilin, such as 14-0-[N-(N-BOC-(R)-vafyl)-1,4,5,6-tetrahydropyridin-4-yl]-su]fanylacetylmutilin, - 14-0-[N-(N-BOC-valyl)-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin, such as 14-0- [N-(N-BOC-(R)-valyl)-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin. In a compound of formula I or of formula tl the group X may be attached to the piperidine or tetrahydropyridine ring in any position with the exception of position 1, e.g. in position 2, 3, 4, 5 or 6, preferably in position 3 or 4. In a compound of formula I or of formula II, the group I, R6, or the group RB respectively, may be in any position with the exception of position 1, of the piperidine or tetrahydropyridine ring, e.g. in position 2, 3, 4, 5 or 6, preferably in position 3 or 4. R8 preferably is alkyl, e.g. (Ci.2o)alkyl, when in position 2 or 6. In a preferred group of compounds of formula I or of formuia II the group X is in position 3 or in position 4; and Re, or the group R8 respectively, is in position 3 or in position 4. In a compound of formula I or of formula II each single substituent may be a preferred substituent, e.g. independently of each other substituent defined. "A residue of an (N-protected) amino acid" as used herein means that in a compound of formula I or of formula II the carbonyl group of said (protected) amino acid is bound to the nitrogen of the group of formula acylated by the carboxylic group of an amino acid. Preferably the residue of an (N-protected)-amino acid is the residue of an (N-protected)-a-amino acid, e.g. a naturally occurring a-amino acid, e.g. (N-protected)-valyl or {N-protected)-histtdinyl, preferably (N-protected)-R-valyl. Compounds provided by the present invention, e.g. a compound of formula I or of formula II, are hereinafter designated as "compound(s) of (or compound(s) according to) the present invention". A compound of the present invention includes a compound in any form, e.g. in free form, in the form of a salt, in the form of a solvate and in the form of a salt and a solvate. Compounds of formula II are useful intermediates in the preparation of compounds of formula I. Compounds of formula II also may show, however, pharmaceutical activity, e.g. similar to that of compounds of formula I. in another aspect the present invention provides a compound of formula I or of formula li in the form of a salt. Such salts include preferably pharmaceutical^ acceptable salts, although pharmaceutical^ unacceptable salts are included, e.g. for preparation / isolation /purification purposes. A salt of a compound of the present invention includes an acid addition salt. Acid addition salts include salts of a compound of formula I or of formula II with an acid, e.g. hydrogen fumaric acid, fumaric acid, naphthalin-1,5-sulphonic acid, hydrochloric acid, deuterochloric acid; e.g. hydrochloric acid or deuterochloric acid, preferably hydrochloric acid. A compound of the present invention may be converted into a corresponding compound in the form of a salt; and vice versa. A compound of the present invention in free form or in the form of a salt and in the form of a solvate may be converted into a corresponding compound in free form or in the form of a salt in non-solvated form; and vice versa. A compound of the present invention may exist in the form of pure isomers or mixtures thereof, e.g. optical isomers, diastereoisomers, cis/trans conformers. A compound of the present invention may e.g. contain asymmetric carbon atoms and may thus exist in the form of enantiomers or diastereoisomeres and mixtures thereof, e.g. racemates. Any asymmetric carbon atom, e.g. to which R6 and X are attached, may be present in the (R)-, (S)- or (Re¬configuration, preferably in the (R)- or (S)-configuration. For example the group bound via the group X to the piperidine ring in a compound of formula I or of formula II may be in the (R)- or in the (S)-configuration or in the form of mixtures thereof. E.g. the amine group of the amino acid residue, e.g. vatyl or histidinyl residue, which is acylating the nitrogen atom of the piperidene ring may be in the (S)-configuration, in the (R)-conftguration or in the form of mixtures therof. Isomeric mixtures may be separated as appropriate, e.g. according to a method as conventional, to obtain pure isomers. The present invention includes a compound of the present invention in any isomeric form and in any isomeric mixture. The present invention also includes tautomers of formula I or of formula II, where tautomers can exist. Preferably the configuration in the mutilin ring of a compound of the present invention is the same as in a naturally produced mutilin. Isomeric mixtures may be separated as appropriate, e.g. according, e.g. analogously, (o a method as conventional, to obtain pure isomers. compound of formula tl, wherein R7 is a protecting group, e.g. BOC, and the other residues are as defined above, b) deprotecting the nitrogen group of the piperidinyl ring in a compound obtained in step a), e.g. by use of etheric HCI, to obtain a compound of formula I, wherein R5 is hydrogen and the other residues are as defined above, c) reacting a compound obtained in step b) with an amino-protected, e,g. BOC-protected, amino acid, e.g. valine or histidine, to obtain a compound of formula II, wherein R7 is the residue of a protected amino acid, e.g. protected valine or histidine, preferably BOC-protected valine or histidine and the other residues are as defined above, d) deprotecting the amino group of the amino acid residue of a compound obtained in step c) to obtain a compound of formula I, wherein R5 is a residue of an amino acid, e.g. valyl or histidinyl; e.g. in the form of a salt, such as a hydrochloride, e) optionally introducing deuterium into a compound of formula I obtained in step d) to obtain a compound of formula I, wherein R2, R3 and R4 are deuterium, and R,, R', and R5 are as defined above, with tbiapleuromultilin and Ai203 to obtain a mixture of compounds of formula II, wherein R7 is a protecting group, e.g. BOC, and wherein in one of the compounds of the mixture the hydroxy group is in position 3 and the sulphur group of the thiapleuromutilin is in position 4 of the piperidine ring, and in the other compound of the mixture the hydroxy group is in position 4 and the sufphur group of the thiapleuromutilin is in position 3 of the piperidine ring. That regioisomeric mixture may be - separated to obtain pure compounds of formula II which pure compounds of formula II may be treated further according to steps b) to f) of the present invention to obtain pure compounds of formula I; or - the regioisomeric mixture of compounds of formula El may be treated further according to steps b) to f) of the present invention to obtain a mixture of corresponding regioisomers of compounds of formula I which mixture may be separated to obtain pure compounds of formula I. Separation of regioisomers may be carried out as appropriate, e.g. by chromatography. If in step A)c) of the present invention the amino acid is used in the (R)-form, e.g.(R)-valine, (R)-histidine, a compound of formula I or II is obtained, wherein the amine group of the (protected) amino acid group attached to nitrogen atom of the piperidine ring is in the (R)-configuration; and if in step A)c) of the present invention the amino acid is used in the (S> form, e.g.(S)-valine, (S)-histidine, a compound of formula I or M is obtained, wherein the amine group of the (protected) amino acid group attached to nitrogen atom of the piperidine ring is in the (S)-configuration. Protecting groups in a production process include appropriate protecting groups, e.g. such as useful in organic chemistry, e.g. (pleuro)mutilin chemistry, e.g. protecting groups as conventional, such as BOC or -CO-CH3. Replacement of hydrogen atoms in a compound of formula I or of formula II, e.g. in the form of a salt, by deuterium atoms may be carried out as appropriate, e.g. according to a method as conventional, e.g. or according to a method described herein, e.g. by treatment of a compound of formula I or of formula II with deuterochloric acid (DCI) in an appropriate solvent (system) and isolation of a compound of formula I or of formula II, e.g. in the form of a salt, wherein hydrogen atoms, e.g. in the meaning of R2, R3 and R4, are replaced by deuterium atoms. The production of a compound of formula I or of formula If, wherein R, and R'i is deuterium may be carried out as appropriate, e.g. according to a method as conventional, e.g. via treatment of a compound of formula wherein the carbon atoms carrying R, and R'i, which both are hydrogen, together form a double bond and wherein R2, R3 and R* are hydrogen, which is a known compound, with deuterium; to obtain a compound of formula IX, wherein R, and R'i are deuterium and R2, R3 and R4 are hydrogen; and further reacting a compound of formula IX, wherein R, and R\ are deuterium and R2, R3 and R4 are hydrogen as appropriate, e.g. according to a method as conventional, to obtain a compound of formula 1 or of formula II, wherein, R^ and R\ are deuterium and R2, R3 and R4 are hydrogen. R is a residue which is chemically not affected by deuterium addition, e.g. -CO-CH2OH. Intermediates in the preparation of compounds of formula I includes compounds of formula III, III", IV, V, VI, VII, VIII or IX, and are known or maybe obtained according to a method as conventional. Any compound described herein may be produced according, e.g. analogously, to a process as conventional, or as described herein. The compounds of the present invention, e.g. including a compound of formula I exhibit pharmacological activity and are therefore useful as pharmaceuticals. The compounds of formula II may be useful intermediates in the preparation of compounds of formula I, which, however, may also exhibit pharmacological activity, e.g. similar to that of compounds of formula I. For example, the active compounds of the present invention (e.g. and compounds of formula II) show antimicrobial, e.g. antibacterial, activity against gram positive bacterias and gram negative bacterias, e.g. gram negative bacterias such as Escherichia coli, and against gram positive bacteria, such as Staphylococcus aureus and in addition Streptococcus pyogenes and Streptococcus pneumoniae, Mycoplasms, Chlamydia, Helicobacter spec, and obligatory anaerobes, e.g. Bacteroides fragilis, in vitro in the Agar Dilution Test or Microdilution Test according to National Commitee for Clinical Laboratory Standards (NCCLS) 1997, Document M7-A4 Vol.17, No. 2: "Methods for dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobicaliy - Fourth Edition, Approved Standard" and e.g. in vivo in systemic infections in mice. The active compounds of the invention show an surprising overall activity spectrum. _ In another aspect the present invention provides a compound of formula I, e.g. or of formula II, for use as a pharmaceutical, preferably as an antimicrobial, such as an antibiotic. For pharmaceutical use a compound of the present invention includes one or more, preferably one, compounds of the present invention, e.g. a combination of two or more compounds of the present invention. In a further aspect the present invention provides the use of a compound of the present invention, e.g. a compound of formula I e.g. or of formula II, for the manufacture of a medicament, e.g. a pharmaceutical composition, for the treatment of a microbial disease, for example of a disease mediated by bacteria, e.g. bacteria selected from Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Mycoplasms, Chlamydia e.g. C. trachomatis and C. pneumoniae and obligatory anaerobes, e.g. including penicillin or multidrug-resistant strains, e.g. of Streptococcus pneumoniae; e.g. including vancomycin-resistant strains, e.g. of Enterococcus faecium; e.g. and including methicillin-resistant strains, e.g. of Staphylococcus aureus and Helicobacter spec, e.g. H. pylori. In another aspect the present invention provides a compound of the present invention or a pharmaceutical composition of the present invention for use in the preparation of a medicament for the treatment of microbial diseases. In a further aspect the present invention provides a method of treatment of microbial diseases which comprises administering to a subject in need of such treatment an effective amount of a compound of the present invention, e.g. a compound of formula I, e.g. or of formula II, e.g. in the form of a pharmaceutical composition. Treatment includes treatment and prophylaxis. For antimicrobial treatment, the appropriate dosage will, of course, vary depending upon, for example, the chemical nature and the pharmakokinetic data of a compound of the present nvention employed, the Individual host, the mode of administration and the nature and severity of the conditions being treated. However, in general, for satisfactory results in larger nammals, for example humans, an indicated daily dosage is in the range from about 0.1 to 3 g, e.g. 0.00125 g/kg to 0.0375 g/kg, of a compound of the present invention conveniently administered, for example, in divided doses up to four times a day. ^ compound of the present invention may be administered by any conventional route, for example enterally, e.g. including nasal, buccal, rectal, oral, administration; parenterally, e.g. including intravenous, intramuscular, subcutanous administration; or topically, e.g. including epicutaneous, intranasal, intratracheal administration, e.g. in form of coated or uncoated tablets, capsules, injectable solutions or suspensions, e.g. in the form of ampoules, vials, in the form of creams, gels, pastes, inhaler powder, foams, tinctures, lip sticks, drops, sprays, or in the form of suppositories, e.g. in analogous manner to macrolides, such as clarithromycin and azithromycin. The compounds of the present invention may be administered in the form of a pharmaceutically acceptable salt, e.g. an acid addition salt or metal salt; or in free form; optionally in the form of a solvate. The compounds of the present invention in the form of a salt exhibit the same order of activity as the compounds of the present invention in free form; optionally in the form of a solvate. A compound of the present invention may be used for pharmaceutical treatment according to the present invention alone or in combination with one or more other pharmaceutical^ active agents. Such other pharmaceutically active agents include e.g. other antibiotics. Combinations include fixed combinations, in which two or more pharmaceutically active agents are in the same formulation; kits, in which two or more pharmaceutically active agents in separate formulations are sold in the same package, e.g. with instruction for co¬administration; and free combinations in which the pharmaceutically active agents are packaged separately, but instruction for simultaneous or sequential administration are given. In another aspect the present invention provides a pharmaceutical composition comprising a compound of the present invention, e.g. a compound of formula I or, e.g. of formula II, in free form or in the form of a pharmaceutically acceptable salt; e.g. and/or in the form of a solvate; in association with at least one pharmaceutical, excipient, e.g. carrier or diluent, e.g. including fillers, binders, disintegrators, flow conditioners, lubricants, sugars and sweeteners, fragrances, preservatives, stabilizers, wetting agents and/or emulsifiers, solubilizers, salts for regulating osmotic pressure and/or buffers. In another aspect the present invention provides a pharmaceutical composition according to the present invention, further comprising another pharmaceutical^ active agent. Such pharmaceutical compositions may be manufactured according, e.g. analogously, to a method as conventional, e.g. by mixing, granulating, coat'mg, dissolving or lyophilizing processes. Unit dosage form may contain, for example, from about 0.5 mg to about 1500 mg, such as ' 1 mg to about 500 mg, e.g. 1 mg to about 100 mg. The compounds of the present invention are additionally suitable as veterinary agents, e.g. veterinary active compounds, e.g. in the prophylaxis and in the treatment of microbial, e.g. bacterial diseases, in animals, such as fowl, pigs and calves; e.g. and for diluting fluids for artificial insemination and for egg-dipping techniques. In another aspect the present invention provides a compound of formula I, e.g. or of formula II, for use as a veterinary agent. In a further aspect the present invention provides a compound of formula I, e.g. or of formula II, for the preparation of a veterinary composition which is useful as a veterinary agent. in another aspect the present invention provides a veterinary method for the prophylaxis and in the treatment of microbial, e.g. bacterial diseases which comprises administering to a subject in need of such treatment an effective amount of a compound of formula I, e.g. or of formula II, e.g. in the form of a veterinary composition. For use of the active compounds of the present invention as a veterinary agent, the dosage will of course vary depending upon the size and age of the animal and the effect desired; for example for prophylactic treatment relatively low doses would be administered over a longer time period, e.g. 1 to 3 weeks. Preferred doses in drinking water are from 0.0125 to 0.05 g/ml, particularly 0.0125 to 0.025 g/ml; and in foodstuffs from 20 to 400 g/metric ton, preferably 20 to 200 g/metric ton. It is preferred to administer the active compounds of the present invention as a veterinary agent to hens in drinking water, to pigs in foodstuff and to calves orally or parenterally, e.g. in the form of oral or parenteral preparations. Ac is hydrogen or (CM2)acy!, e.g. a group -CO-CH3, with the proviso that if X is S and one of the dotted lines is a group OAc and the other dotted line is no bond, then Ac is other than hydrogen. Example 1 14-0-[N-BOC-4-Hydroxy-piperidin-3-yI]-sulfanylacety)muti)inand14-0-[N-BOC-3- hydroxy-piperidin-4-yl]-sulfanylacetylmutilin 40 g of (neutrally) activated Al203, moistened with THF, are Ireated with a solution of 1.576 g of thiapleuromutiline in 5 ml of THF and to the mixture obtained 0.398 g of N-BOC-3,4-epoxy-piperidine, dissolved in 3 ml of THF, are added. From the mixture obtained Af203 is filtered off, from the filtrate obtained solvent is evaporated off and the evaporation residue comprising a mixture of 14-0-[N-BOC-4-hydroxy-piperidin-3-ylI-sulfanylacety[mutilin and 14-0-[N-Boc-3-hydroxy-piperidin-4-yl]-sulfanylacetylmutilin is subjected to chromatography. 14-0-[N-BOC-3-Hydroxy-piperidin-4-yl]-sulfanylacetylmutilin and 14-0-[N-BOC-4-Hydroxy-piperidin-3-yl]-sulfanylacetylmutilin are obtained. 14-0-[N-BOC-3-hydroxy-piperidin-4-yl]-sulfany!acetylmutilin is also obtained by reacting 0.466 g of N-BOC-3-hydroxy-4-mercaptopiperidine in 10 ml of THF with 0.224 g of tert.But-OK in 20 ml of THF, adding to the mixture obtained of a solution of 1.064 g of 22-0-tosylpleuromutilin in 5 ml THF, dropwise adding to the mixture obtained 1 ml of 2-butanone, stirring at RT and subjecting to chromatographic purification. Example 2 14-0-[4-Hydroxy-N-(N-BOC-valyl-piperidin-3-ylJ-sulfanylacetylmutilin 1.5 mmol of 14-0-[4-hydroxy-piperidin-3-yl]-sulfanylacety!mutilin dissolved in 5 ml of CHZCI2 are treated with 1.5 mmol of HOBT, 1 mmol of (R)-BOC-valin and 1.5 mmol of EDC and stirred at RT. From the mixture obtained solvent is evaporated, the evaporation residue obtained is mixed with EE and the mixture obtained is extracted with 0.1 N HCI and saturated aqueous NaHCOa solution. The organic phase obtained is dried and solvent is evaporated. ■I4-0-[4-Hydroxy-N-(N-BOC-(R)-valyl-piperidin-3-yl-sulfanylacetylmutilin is obtained. Example 3 14-0-[4-Hydroxy-N-(R)-valyl)-piperidin-3-yl]-sulfany!acetylmutilin 1 mmol of 14-0-[4-hydroxy-N-(N-BOC-(R)-valyl-piperidin-3-yl-sulfanylacetylmutilin in 5 to 8 ml of CH2CIS is treated with 1 to 2 ml of etheric HCI, the mixture obtained is stirred at RT and 14-0-[4-Hydroxy-N-(R)-valyl)-piperidin-3-yl]-sulfanylacetylmutilin in the form of a hydrochloride precipitates and is isolated by filtration. Example 4 14-0-[N-(N-BOC-valy{)-1,2,3,6-tetrahydropyrl a) 3-Mesvloxv-N-(N-BOC-(RVvalvll-1,2,3.6-tetrahvdropyridine 0.894 g of N-(N-BOC-(R)-va!yl)-1,2,3,6-tetrahydropyridin-3-ol dissolved in 10 ml of CH2CI2 are treated with 0.844 g of 4-dimethylaminopyridine and 0.31 g of methanesulfonic acid chlorid (mesylchloride) and stirred for ca. 24 hours, the mixture obtained is treated with 0.1N HCI and CH2CI2, the organic phase otained is washed with H20 and aqueous NaHC03-solution, the solvent is evaporated and the evaporation residue is dried. 3-Mesyloxy-N-(N-BOC-(R)-valyl)-1,2,3,6-tetrahydropyridine is obtained. 1H-NMR (CDCI3): 6.1-5.85(m,2H,H,v,Hv), 4.5(m,1H,NHCHCO), 3.7(s,3H,CH3S02), 1.2-0.9(m,6H,(CH3)2. b)14-0-rN-fN-BOC-(R)-valvn-1.2,3,6-tetrahvdropvridin-3-vl1-sulfanvlacetvlmutilin 0.235 tert.But-OK dissolved in 5 ml of THF are treated with thiapleuromutilin in 10 ml of THF and to the mixture obtained 0.789 g of 3-mesyloxy-N-(N-BOC-(R)-valyl)-1,2,3,6-tetrahydropyridine in 10 ml of THF are added dropwise. The mixture obtained is heated to 90" and stirred at RT. The mixture obtained is treated with diluted aqueous HCI, the organic phase obtained is washed and solvent is evaporated. 14-0-[N-(N-BOC-(R)-valyl)-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin is obtained. Example 5 14-0-[N-BOC-1A5,6-tetrahydropyridin^y|]-sulfanylacetylmutilin 2.72 ml of diisopropylamine in 40 ml of THF are treated with 12 ml n-butyl-lithium (1.6 M solution in hexane) at -40° and the mixture obtained is stirred, warmed to -10° and a solution of 3.44 g of N-BOC-1,2,5,6-tetrahydropyridine in 20 ml of THF is added dropwise. To the mixture obtained a solution of 22-O-tosylpleuromutilin in 10 ml of THF and 1 ml of 2-butanone are added and the mixture obtained is stirred at RT. The mixture obtained comprising a mixture of 14-0-[N-BOC-1,4,5,6-fetrahydropyridin-4(R')-yl]-sulfanylacefyl-mutilin (COMPOUND A) and 14-0-[N-BOC-1,4,5,6-tetrahydropyridin-4(S*)-yl]-sulfanylacetylmutilin (COMPOUND B) is subjected to chromatography and pure COMPOUND A and pure COMPOUND B are obtained. Example 6 14-0-[N-(N-BOC-valyl)-1,4,5,6-tetrahydropyridin-4-yl]-sulfanylacetylmutilin 4.53 ml of diisopropylamine in 30 ml of THF are treated with n-butyl-lithium (1.6 M solution in n-hexane) at -40X. The mixture obtained is stirred, warmed up to -10° and a solution of 5.02 g of 3,4-epithio-N(N-BOC-(R)-va1y1)-piperidine in 30 ml of THF is added. The mixture obtained is stirred for ca. 3 hours at -10°, a solution of 22-0-tosylpieuromutilin in 20 ml of THF and 5 ml of 2-butanone are added and the mixture obtained is stirred at RT. The mixture obtained is subjected to extractive work up and chromatography. 14-O-[N-(N-B0C-(R)-valyl)-1,4,5,6-tetrahydropyridin-4-yl]-sulfanylacetylmutilin is obtained. A mixture of 106.4 g of 22-O-tosylpleuromutilin, 15.2 g of thiourea and 250 ml of acetone is refluxed for ca. 1.5 hours, cooled and from the mixture obtained solvent is evaporated and the evaporation residue is dried in vacuo. Thiapleuromutilin in the form of an isothiuronium salt is obtained. 1H-NMR: 9.82,8.42(2xb,2H,NH2),7.78, 7.2(2xd,4H,arom.HTosyi,J=15.8Hz) a) Thiapleuromutilin 24.4 g of thiapleuromutilin in the form of an isothiuronium salt, dissolved in 40 ml absolute EtOH, is diluted with 70 ml of H20 and warmed to 90°. The mixture obtained is treated with 7.6 g of sodium disulfite in 35 ml of H20 and to the mixture obtained 200 ml of CH2CI2 are added. The mixture obtained is heated to 90° for ca. 1.5 hours and cooled. Two phases are formed and are separated, the organic phase obtained is washed, dried, solvent is evaporated and the evaporation residue is filtered through silicagel. Thiapleuromutilin is obtained. Example B - N-BOC-3,4-Epoxy-piperidine a) N-BOC-1.2,5,6-tetrahvdropyridine To 1.66 g of 1,2,5,6-tetrahydropyridine in 25 ml of CH2CI2, 2.02 g of N-methylmorpholine are added, the mixture obtained is treated with a solution of 4.36 g (BOC)20 in 30 ml of CH2CI2 and the mixture obtained is stirred for ca. 36 hours at RT. N-BOC-1,2,5,6-tetrahydropyridine is obtained. 1H-NMR: 5.82{m,fH,Hlv), 5.64 b) N-BOC-3.4-Epoxv-piperidine To a solution of 3.29 g of N-BOC-1,2,5,6-tetrahydropyridine in 25 ml of CH2CI2, a suspension of 6.2 g of chloroperbenzoic acid in 50 ml of CH2Cl2 are added and the mixture obtained is stirred for ca. 12 hours at RT. The mixture obtained is extracted with saturated aqueous NaHC03-so!ution and 0.5 m aqueous Na2Ss03-solution and the organic phase obtained is washed, dried and the solvent is evaporated. N-BOC-3,4-epoxy-piperidine is obtained. 'H-NMR: 3.9, 3.65, 3.45, 3.1(4xm,4H,H„,Hvi), 3.28, 3.2 (2xm,2H,Hm,H|V), 2.05, 1.9(2xm,2H,Hv), 1.45(s,9H,(CH3)3). Example C - N-{N-BOC-(R)-vaIyl)-1,2,3,6-tetrahydropyridin-3-ol a) N-fN-BOC-valvl-1.2.5.6-tetrahydropyridine 1.245 g of tetrahydropyridine in 50 ml of CHZCI2 are treated with 1.5 mmol per mrnol of tetrahydropyridine of HOBT, 2.17 g of N-BOC-(R)-valin and 1.5 mmol per mmol of tetrahydropyridine of EDC and (he mixture obtained is stirred at RT. From the mixture obtained solvent is evaporated, the evaporation residue obtained is mixed with EE and the mixture obtained is extracted with 0.1N HCI and saturated aqueous NaHC03 solution. The organic phase obtained is dried and solvent is evaporated. N-(N-BOC-(R)-valyl-1,2,5,6-tetrahydropyridine is obtained. b) 3,4-EPOXV-N-(N-BOC-valvl-1.2,5.6-tetrahvdropvridine To a solution of 2.82 g of N-(N-BOC-(R)-valyl-1,2,5,6-tetrahydropyridine in 75 ml of CH2CI2, 3.44 g of m-chloroperbenzoic acid in 50 ml of CH2CI2 are slowly added and the mixture obtained is stirred overnight. The mixture obtained is extracted with aqueous NaHC03-solution and with 0.5 m aqueous Na2S203-solution, the phases obtained are separated and from the organic phase solvent is evaporated in vacuo. 3,4-Epoxy-N-(N-BOC-(R)-valyl-1,2,5,6-tetrahydropyridine is obtained. 'H-NMR: Rotameres: 5.3(m,1 H,NHCHCO), 4.4(m,1 H, NHCHCO), 4.3, 4.1, 4.0 (Sdd.lH.Hm.J^IS.eHz), 3.88, 3.78, 3.65(3xd,1H,H|V,J=15.6Hz), 3.6, 3.45, 3.3(3xm,4H,H„, HV(). 1.45(b,9H(CH3)3), 1.0-0.85(m,6H,CH(CH3)2). c) Bromo-N-(N-BOC-valvl)-piperidin-3-ol 0.5 g of Ph3PBr2 in 10 ml of CH2CI2 are treated with 0.289 g of 3,4-epoxy-N-(N-BOC-(R)-valyM ,2,5,6-tetrahydropyridine in 10 ml of CH2CI2. The mixture obtained is poured onto a mixture of ice/NaHC03, the organic phase is separated, washed, dried and solvent is evaporated. A mixture of 4(R')-bromo-N-(N-BOC-(R)-valyl)-piperidin-3(R*)-ol (COMPOUND A) and 4{S*)-bromo-N-(N-BOC-(R)-valyl)-piperidin-3(S*)-ol {COMPOUND B) is obtained and separated by chromatography. COMPOUNDA:1H-NMR:Rotameres:5.2(m,1HNHCHCO),4.3(t,1H,NHCHCO,J=6.5Hz),4.25 {m,1H,H|V),3.88(m,1H,H||i),2.4,1.85(2xm,2H,Hv),1.43(b,9H(CH3)3),0.98,0.92(2xd,6H, CH(CH3)2,J=7Hz). COMPOUND B:1H-NMR: Rotameres: 5.25(d,1H,NHCHCO,J=6.7Hz), 4.45(m,1H,NHCHCO), *.15(m,1H,H,v), 3.75(m,1H,Hm), 2.55, 2.3(2xm,2H,Hv), 1.9(m,1H,CH(CH3)2), 1.42 (b,9H [CH3)3), 0.9(m,6H,CH(CH3)3). d) 3-Acetoxy-4-bromo-N-(N-BOC-valyl)-piperidine 0.57 g of bromo-N-(N-BOC-valyl)-piperidin-3-ol, dissolved in pyridine, is treated with 0.4 ml of acetic acid anhydride, the mixture obtained is stirred and a mixture of 3(R*)-acetoxy-4(R*)- bromo-N-{N-BOC-(R)-valyl)-piperidine (COMPOUND A) and 3(S*)-acetoxy-4(S*)-bromo-N- (N-BOC-(R)-valyl)-piperidine (COMPOUND B) is obtained and is separated by chromatography. f) N-( N-BOC-( RVva Ivl H. 2.3,6-tetrahvd ropyridin-3-ol 0.254 g of 3-acetoxy-N-{N-BOC-(R)-valyl)-1,2,3,6-tetrahydropyridine, dissolved in 5 ml of EtOH are treated with 2N ethanolic NaOH under ice-cooling. To the mixture obtained acetic acid is added in order to neutralize the reaction mixture and solvent is evaporated. The evaporation residue obtained is mixed with CHCI3, the mixture obtained is washed with NaCI-solution, the organic phase is dried and solvent is evaporated. N-(N-BOC-(R)-valyl)-1,2,3,6-tetrahydropyridin-3-ol is obtained. 1H-NMR: 5.9(m,2H,Hw, Hv), 4.51, 4.45(2xdd,1H, NHCHCO,J=5.2Hz,J=9.0Hz), 1.4 (b,9H,(CH3)3), 0.9(m,6H,CH(CH3)j). Example D - Methylaminoacetylmutilin 3.33 g of 22-O-tosylpleuromutilin in 350 mi of EtOH are treated with 5 ml CH3NH2 (33% otution in EtOH), the mixture obtained is refluxed for ca. 30 hours and from the mixture ibtained solvent is evaporated. The evaporation residue is treated with EE and the mixture ibtained is extracted with 0.1N HCI. The aqueous phase obtained is treated with NaHC03 md extracted with EE. The organic phase obtained is dried and solvent is evaporated, tethylaminoacetylmutilin is obtained. 1H-NMR: AB-system: VA=3.32IVB=3.22(2H,H22, WH3=15HZ), 2.42(S,3H,CH3NH). Example E 4-BOC-1,2,5,6-tetrahydropyridine 1.66 g of 1,2,5,6-tetrahydropyridine in 25 ml of CHjCI2 are treated with 2.02 g of N-methyl-norpholine. To the mixture obtained 4.36 g of (BOC)20 in 30 ml of CH;>CI2 are added and he mixture obtained is left for reaction for ca. 36 hours. The mixture obtained is subjected to aqueous extraction, the organic phase is dried and evaporated. N-BOC-1,2,5,6-tetrahydro¬pyridine is obtained. 1H-NMR: 5.82(m,1H,H,v), 5.64{m,1H,H||i), 3.86{b,2H,H„), 3.47(t,2H,Hv,), M2{b,1H,Hv), 1.46(m,9H,(CH3)3). Example F i,4-Epithio-N(N-BOC-valyl)-piperidine 2.91 g of KSCN in 3 ml of H20 are added to a mixture of 5.96 g of 3,4-epoxy-N-(N-BOC- •alyl-1,2,5,6-tetrahydropyridJne in 10 ml of absolute EtOH and the mixture obtained is stirred for 72 hours at RT. The mixture obtained is subjected to aqueous extraction, the solvent of !he organic phase obtained is evaporated and the evaporation residue is subjeted to chromatography. 3,4-Epithio-N(N-BOC-(R)-valyl)-piperidine is obtained. Melting point: 69.71° WE CLAIM: 1. A compound of formula wherein Ri and R-t are hydrogen or deuterium, R2, R3 and R* are hydrogen or deuterium, R5 is the residue of an amino acid, X is S or N-ALK, 2. The compound according to claim 1 which is selected from the group consisting of 14-0-[4-hydroxy-N-valyi-piperidin-3-yl]-sulfanylacetylmutilin, 14-0-(3-hydroxy-N-valyl-piperidin-4-yl]-sulfanylacetylmutilin, 14-0-[3-hydroxy-N-histidinyl-piperidin-4-ylI-sulfanylacetylmutilin, 14-0-(3-hydroxy-N-vafyl-piperidin-4-yl]-methylaminoacetylmutilin, 14-0-(4-bydroxy-N-valyl-piperidin-3-yl]-methy!amJnoacetylmut)lin, 14-0-tN-valyl)-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin, and 14-0-[N-valyl)-1,4,5,6-tetrahydropyridin-4-yl]-sulfanylacetylmutilin. 4. The compound according to claim 3 selected from the group consisting of 14-0-[N-BOC-4-hydroxy-piperidtn-3-ylI-su[fany(acetylmutilin, 14-0-[N-BOC-3-hydroxy-piperidin-4-ylI-sulfanylacetylmutitin, 14-0-[4-hydroxy-N-BOC-piperidin-3-yl]-methylaminoacefylfnutiftn, 14-0-[3-hydroxy-N-BOC-piperidin-4-yl]-methylaminoacetylmuti(in, 14-0-[N-BOC-1,4,5,6-tetrahydropyridin-4-yJ]-su]fanylacetylmutilin, 14-0-[4-hydroxy-N-(N-BOC-vatyl)-piperidin-3-yl]-sulfanylacetylmutilin, 14-0-[3-hydroxy-N-(N-BOC-valyl)-piperidin-4-ylJ-sulfanylacetylnnutilin, 14-O-[4-acetoxy-N-(N-B0C-valyl)-piperidin-3-yll-sulfanylacetylmutilin, 14-0-[3-acetoxy-N-(N-BOC-valyl)-piperidin-4-ylJ-sulfanytacetyfmutifin, 14-0-{3-hydroxy-N-(N-BOC-histidinyl)-piperidin-4-yl]-sulfanylacetylmutilin, 14-0-[3-hydroxy-N-(N-BOC)-valyl-piperidin-4-yl]-methylami noacetyl mutilin, 14-0-I4-hydroxy-N-(N-BOC)-valyl-piperidin-3-yl]-methylaminoacety!mutiHn, 14-0-[N-(N-BOC-vafyf)-1,4,5,6-tetrahydropyridin-4-y(J-su)fanyJacetylmutilin, 14-0-[N-(N-BOC-va!y!)-1,2,3,6-tetrahydropyridin-3-yl]-sulfanylacetylmutilin. 5. The compound of any one of claims 1 to 4 in the form of a salt. 6. The compound of any one of claims 1 to 5 as a pharmaceutical. 7. T he compound of any one of claims 1 to 5 in the preparation of a medicament for the treatment of microbial diseases.

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3123-chnep-2004 abstract.pdf

3123-chnep-2004 claims duplicate.pdf

3123-chnep-2004 claims.pdf

3123-chnep-2004 correspondence-others.pdf

3123-chnep-2004 correspondence-po.pdf

3123-chnep-2004 description (complete) duplicate.pdf

3123-chnep-2004 description (complete).pdf

3123-chnep-2004 form-1.pdf

3123-chnep-2004 form-18.pdf

3123-chnep-2004 form-3.pdf

3123-chnep-2004 form-5.pdf

3123-chnep-2004 form-6.pdf

3123-chnep-2004 petition.pdf

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