Title of Invention

A process for the removal of heavy metals from aqueous solution

Abstract

A process for the removal of heav/y metals from aqueous solution which comprises growing alkalophilic actinomycetes strain in a medium containing carbon and nitrogen metal ions in a temperature range of 20°C to 45°C and pH in the range of 7.5 to 11 for a period in the range of 1-4 days with constant stirring removing the heavy metal from the biomass by the use of dilute acid and recovering the pure waste water by conventional filtration methods.

Full Text

M process TOT me removal ui neavy meiais um Field of the invention The present invention relates to a process for the removal of heavy metal ions from aqueous solutions using metal tolerant alkalophilic actinomycete. Background of the invention Rapid and large scale industrialization has led to generation of large quantities of waste water. Waste water from many industrial processes such as metal finishing, electroplating, paint and the like contain several heavy metal ions which are toxic in nature and harmful to the environment while a few of such metal ions such as gold or silver or platinum and the like are valuable. It is, therefore, extremely important to remover metal ions from wastes in order to control pollution and recover valuable metals. The following are major sources of heavy metal pollution : (a) mining and processing of minerals; (b) nuclear and other industrial effluents; (c) brewing and distillery wastes; (d) biocides and preservatives including organo metallic compounds; (e) leather industry: (f) metal refining and metal cleaning; (g) electroplating, metal processing and dyeing industry, and the like. Heavy metals which commonly occur as pollutants in effluents are Cu, Zn, Cd, Fe, Hg, Pb, Ni, Ag, Au and radionuclides such as U, Th and the like. According to the US Environment Protection Agency, the acceptable value of heavy metal ion is usually less than 1 mg/L (1ppm). Although the total amount of metals released by such activities is large, concentrations are typically low rendering most prior art recovery techniques impractical. The existing processes for removal of heavy metals include solvent extraction, hydroxide precipitation (lime addition), evaporative concentration, electrowinning, membrane concentration, ion exchange processes, adsorption, reverse osmosis and biological methods. However, all the above methods known in the art suffer from several disadvantages. The prior art methods mentioned above require high energy, have limited applicability, low adsorption capacity, etc. As an example, chemical precipitation produces wet bulky sludge and requires a filter for the polishing stage. Ion exchange adsorption, reverse osmosis and solvent extraction are not only relatively expensive and involve elaborate and costly equipment but also the operation costs are very energy intensive. Several attempts have been made to use microbial biomass for metal removal (Volesky and Holan, 1995; Rani Gupta et al., 2000). Living and non-living cells have been used to concentrate metal ions from aqueous solutions which is a rapid and reversible phenomenon. US Patent 4320093 (1982) discloses a technique for the removal of U and Th from solutions using a fungal microorganism Rhizopus arrhizus. Koglev et al report the use of bioabsorbents prepared from Aspergillus, Penicillium, Trichoderma or Micrococcus biomass (US Patent 5789204. 1982). Dick and Macasakie report the use of Acinetobacter for removal of metals by the precipitation of the metal as insoluble metal phosphates due to the phosphate produced by the organism by enzymatic reaction (US Patent 5520811, 1996). Brierley et ai describe the preparation of biomass product by alkaline treatment to increase the metal uptake by several folds. Among the various bacteria and fungal cultures tested B. subtilis was found to be best (US Pjtejit^6^l894,_ 1987). Volesky describes the immobilized biomass of a marine alga for the removal of gold (US Patent 4769223. 1988). Biosorption of metals is due to the binding of metal ions to various cell constituents, primarily the cell wall. As organisms vary widely in cell wall composition, their capacity to bind metals also varies. Thus different organisms bind metals to different extents. While bacteria, fungi, yeast and algae have been studied for heavy metal removal, there are very few reports on the use of mycelial actinomycetes (Mattuschka and Straube, 1993; Kefala et al, 1999) and fewer where live actinomycetes cultures have been employed. No reports have been located in prior art literature relating to the use of alkalophilic or alkalotolerant actinomycete for heavy metal biosorption, bioaccumulation or removal. Objects of the invention The main object of the invention is therefore to provide a process for the removal of heavy metal ions from media/aqueous solutions using alkalophilic or alkalotolerant actinomycetes. It is another object of the invention to provide a process for the removal of toxic heavy metal ions from media/aqueous solutions using alkalophilic or alkalotolerant actinomycetes thereby reducing the level of pollution in waste water and effluent streams. It is another object of the invention to provide a process for the recovery of valuable heavy metal ions such as gold, silver and platinum from media/aqueous solutions using alkalophilic or alkalotolerant actinomycetes. Summary of the invention The present invention relies on the ability of alkalophilic actinomycete to tolerate and adsorb heavy metals during growth. Significant quantities of heavy metals are also taken up from aqueous solutions by pre-grown biomass. The term 'biomass' as used herein refers to the cellular mass of the microorganism produced as a result of growth. Accordingly, the present invention provides a process for the removal of heavy metals from aqueous solution which comprises growing alkalophilic actinomycetes strain in a medium containing carbon and nitrogen metal ions in a temperature range of 20°C to 45°C and pH in the range of 7.5 to 11 for a period in the range of 1-4 days with constant stirring removing the heavy metal from the biomass by the use of dilute acid and recovering the pure waste water by conventional filtration methods. In one embodiment of the invention, the heavy metal uptake from the aqueous solution is achieved by contacting the said aqueous solution with the alkalophilic or alkalotolerant actinomycete biomass. In another embodiment of the invention the heavy metal uptake from the aqueous solution is achieved by growing the alkalophilic or alkalotolerant actinomycete in said aqueous solution. In another embodiment of the invention, the alkalophilic actinomycete comprises NCIM 5080. In another embodiment of the invention, the heavy metal ion is selected from the group comprising of Co, Cu, Cr, Ni, Pb, Zn, Ag, Cd, Pt, Au and any mixture thereof. In another embodiment of the invention, the process comprises growing NCIM 5080 in a medium containing carbon and nitrogen sources and metal ions selected from the group consisting of cobalt chloride, cupric chloride, potassium dichromate, nickel nitrate, lead nitrate, zinc sulphate and any mixture thereof, in a temperature ranging between 20-45°C and a pH in the range of 7.5 to 11 for a period of 1-4 days with constant stirring. In another embodiment of the present invention, the process comprises incubating the said metal solutions singly or in combination with a pre-grown biomass of NCIM 5080 at a temperature in the range of 15-50°C at a pH in the range of 3-11 for 10 minutes to 24 hours with shaking/stirring. MT: T7 In another embodiment of the invention, the heavy metal is recovered from the biomass by the use of dilute acids or salts or chelating agents. In a further embodiment of the invention, the dilute acid is selected from the group consisting of hydrochloric acid, lactic acid, nitric acid and sulphuric acid. In a further embodiment of the invention, the salts are selected from the group consisting of sodium chloride, MgSO4 and Na2SO4. In a further embodiment of the invention, the chelating agent comprises EDTA. Brief description of the accompanying drawings Figure 1 is a graphical representation of the effect of nickel concentration in the biomass in accordance with example 3. Figure 2 is a graphical representation of the effect of pH on biosorption of nickel in accordance with example 4. Figure 3 is a graphical representation of the effect of co-ions on the adsorption on Ni in the biomass in accordance with example 5. Figure 4 is a graphical representation of the effect of Co-ions on the uptake of Zn in accordance with example 7. Detailed description of the invention The invention relates to a process for the removal of heavy metal ions using an alkalophilic actinomycete isolate. The isolate NCIM 5080 grows at alkaline pH and can tolerate and grow in the presence of high levels of heavy metals such as chromium ions and produce alkaline protease (Indian Patent Applications 373/Del/94 and 431/Del/97). This organism can also tolerate other heavy metal ions such as Co, Cu, Ni, Pb and Zn. The published reports on heavy metal removal pertain predominantly to studies on bacteria growing at neutral or slightly alkaline pH. There are no reports on tolerance to heavy metals or accumulation by mycelial actinomycetes. Actinomycete designated NCIM 5080 is isolated from its natural habitat of Sambhar Lake, Rajasthan, India. The culture grows at alkaline pH in the presence of high concentration of Cr (up to 5000 ppm) supplemented either as Cr3+ or Cr6+ to the medium. NCIM 5080 formed whitish to pale cream coloured aerial mycelium on alkaline mikami agar (g/L: beef extract 5; peptone 5, yeast extract 1.5 and glucose 1.5). Cell wall analysis indicated the presence of only m-DAP. The culture grows well between pH 7 - 11 and 25 to 45°C and utilises various inorganic sources such as NaNO3, KNO3, Ca(NO3)2, NH4NO3, (NrUfeSCX as well as organic nitrogen sources such as peptone, yeast extract, casein, soyabean meal, sugars such as glucose, galactose, mannose, and sugar alcohols such as glycerol, mannitol, sorbitol and starch as carbon sources. The morphological characteristics of the organism when grown on various agar media are provided in Table 1 below: Table I: morphological and cultural characteristics of organism grown on different agar cultures (Table Removed) pH of the medium made alkaline by addition of sterile sodium carbonate The heavy metal uptake from the aqueous solution is achieved by contacting the said aqueous solution with the alkalophilic or alkalotolerant actinomycete biomass or by growing the alkalophilic or alkalotolerant actinomycete in said aqueous solution. The heavy metal ion can be any of Co, Cu, Cr, Ni, Pb, Zn, Ag, Cd, Pt, Au and any mixture thereof. In one feature of the invention NCIM 5080 is grown in a medium containing carbon and nitrogen sources and metal ions selected from cobalt chloride, cupric chloride, potassium dichromate, nickel nitrate, lead nitrate, zinc sulphate and any mixture thereof, in a temperature ranging between 20 - 45°C and a pH in the range of 7.5 to 11 for a period of 1 - 4 days with constant stirring. The metal solutions singly or in combination can also be incubated with a pre-grown biomass of NCIM 5080 at a temperature in the range of 15 - 50°C at a pH in the range of 3 - 1 1 for 10 minutes to 24 hours with shaking/stirring. The heavy metal is recovered from the biomass by the use of dilute acids such as hydrochloric acid, lactic acid, nitric acid and sulphuric acid or salts such as sodium chloride, MgSO4 and Na2SO4 or a chelating agent such as EDTA etc. The removal of nickel above 80 - 90 % (loading capacity between 26 - 32 mg/g) is many fold higher when compared to 5mg/g reported for Streptomyces noursei reported by Mattuschka and Straube (1993). Similarly, the zinc uptake of 30 - 35 mg/g of native biomass is comparable to values reported in prior art (Puranick and Pakniker, 1995: Mameri et al, 1999). These values can be further increased by appropriate pre-treatment of the biomass. The ability of the biomass to remove Ni and Zn from the mixture of other heavy metals is advantageous as the effluents often contain more than one heavy metal. Example 1 (comparative) Spores from 4-7 day old alkaline Mikami slant were transferred to liquid medium of the same composition (Indian Patent Application Nos. 343/Del/94; 431/Del/97). After 24 hours of growth, 10% (v/v)vegetative growth was transferred to alkaline liquid medium containing 20 -100 ppm of Co, Cu, Cd, Cr. Ni, Pb, Zn singly or in combination and incubated at 28°C, with shaking at 180 - 220 rpm. The organism grew well without significant inhibition and the biomass dry weight ranged between 1.5 - 2.5 g/L. the uptake of the metal depended on the metal ion as is evident from Table 2. Table 2: Uptake of metals during growth of NCIM 5080 (Table Removed) Example 2 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionized water and adjusted to pH of 6 - 7. It was then mixed with one of the following metal solutions (100 ppm effective concentration) viz. silver, cobalt, copper, nickel, zinc (adjusted to pH 5 - 7) and incubated with shaking at 28°C. After one hour, the biomass was filtered, washed thoroughly and metal content in the biomass was estimated by atomic absorption spectrometer after acid digestion of the biomass. The removal of silver, copper cobalt from the solution ranged from 60 - 82% (see Table 3). Table 3: (Table Removed) Example 3 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionised water and adjusted to neutral pH. It was then mixed with nickel solution (to give varying effective concentrations of 10 - 100 ppm) and incubated with shaking at 28°C. After one hour, the biomass was filtered, washed thoroughly and nickel content in the biomass was estimated by atomic absorption spectrometer after acid digestion of the biomass. The metal content in the biomass increases with increasing concentration and 70 - 95% nickel was removed from the solution, (figure 1). Example 4 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionized water and adjusted to different pH in the range of 3 - 8. It was then mixed with nickel solution of corresponding pH (adjusted previously in the range of 3 - 8) and incubated with shaking at 28°C. After one hour, the bioma J was filtered, washed thoroughly and nickel content in the biomass was estimated using atomic absorption spectrometer after acid digestion of the biomass. The metal content in the biomass increases with increase in pH up to 7 and decreased with further increase. About 90% of the Ni was removed from the solution within 1 hour. (Figure 2). Example 5 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionized water and adjusted to pH 7. It was then mixed with nickel solution containing one or more heavy metals adjusted to pH 7 and incubated with shaking at 28°C. After one hour, the biomass was filtered, washed thoroughly and nickel content in the biomass was estimated using atomic absorption spectrometer after acid digestion of the biomass. The nickel content in the biomass is unaffected by the presence of other metals such as Co, Cr, Pb, and Zn in the solution. (Figure 3). Example 6 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionized water and adjusted to pH 6. It was then mixed with zinc solution adjusted to pH 6 and incubated with shaking at 28°C. After one hour, the biomass was filtered, washed thoroughly and zinc content in the biomass was estimated using atomic absorption spectrometer after acid digestion of the biomass. The zinc content in the biomass and percentage zinc removal from the aqueous solution ranged between 10-35 mg/g and 70 to > 90% respectively depending on the initial zinc concentration (25-100 ppm). Example 7 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionized water and adjusted to pH 7. It was then mixed with a mixture of metal containing zinc (adjusted to pH 7) and incubated with shaking at 28°C. After one hour, the biomass was filtered, washed thoroughly and zinc content in the biomass was estimated using atomic absorption spectrometer after acid digestion of the biomass. The zinc content in the biomass was around 14 mg/g and zinc removal was not affected by the presence of other metals such as Ni, Cd, Cr, Pb and > 90 % zinc was removed from the solution. (Figure 4). Example 8 NCIM 5080 was grown in liquid alkaline Mikami for 24 hours. The biomass was filtered, washed thoroughly and suspended in deionized water and adjusted to pH 7. It was then mixed with 100 ppm of nickel and incubated with shaking at 28°C. After one hour, the biomass was filtered, washed thoroughly and nickel content in the biomass was estimated using atomic absorption spectrometer after acid digestion of the biomass. The nickel in the biomass was recovered by incubating with an eluent for 2 hours with shaking. The recovery of nickel was between 50 - 98% depending on the choice of eluent as given in Table 4. Table 4: Recovery of Ni from biomass (Table Removed) Advantages of the invention 1. The process of the invention results in reduction of pollution due to the absence of toxic metals such as Ni in the effluent stream 2. The process also provides an economical route to recover precious metals such as gold, silver or platinum in good yield. We claim : 1. A process for the removal of heavy metals from aqueous solution which comprises growing alkalophilic actinomycetes strain in a medium containing carbon and nitrogen metal ions in a temperature range of 20°C to 45°C and pH in the range of 7.5 to 11 for a period in the range of 1-4 days with constant stirring removing the heavy metal from the biomass by the use of dilute acid and recovering the pure waste water by conventional filtration methods. 2. A process as claimed in claim 1 wherein the heavy metal ion is selected from the group comprising of Co, Cu, Cr, Ni, Pb, Zn, Ag, Cd, Pt, Au and any mixture thereof. 3. A process as claimed in claims 1-2 wherein the said carbon sources are selected from the group consisting of sugars comprising at least one of glucose, galactose and mannose, sugar alcohols comprising at least one of glycerol, mannitol and sorbitol, and starch. 4. A process as claimed in claims 1-3 wherein the said nitrogen source is selected from the group consisting of NaNO3, KNO3, Ca(NO3)2, NH4NO3, (NH4)2SO4, peptone, yeast extract, casein and soyabean meal. 5. A process as claimed in claims 1-4 wherein the process comprises incubating the said metal solutions singly or in combination with a pre-grown biomass of NCM 5080 at a temperature in the range of 15-50°C at a pH in the range of 3-11 for 10 minutes to 24 hours with shaking/stirring. 6. A process as claimed in claims 1-5 wherein the heavy metal is recovered from the biomass by the use of dilute acids or salts or chelating agents selected from the groups consisting of hydrochloric acid, lactic acid, nitric acid and sulphuric acid; sodium chloride, MgSO4 and Na2SO4; and EDTA respectively. 7. A process for the removal of heavy metals from aqueous solution substantially as herein described with reference to the examples.

Documents:

1373-delnp-2003-abstract.pdf

1373-delnp-2003-claims.pdf

1373-delnp-2003-correspondence-others.pdf

1373-delnp-2003-correspondence-po.pdf

1373-delnp-2003-description (complete).pdf

1373-delnp-2003-drawings.pdf

1373-delnp-2003-form-1.pdf

1373-delnp-2003-form-19.pdf

1373-delnp-2003-form-2.pdf

1373-delnp-2003-form-3.pdf

1373-delnp-2003-form-5.pdf

1373-delnp-2003-petition-137.pdf