|Title of Invention||
COMPOSITION COMPRISING STATINS
|Abstract||A composition comprising statin wherein the composition is a flour comprising less than 10 wt.% of fat and the use of the composition comprising statin in the preparation a food product is disclosed. The process for the preparation of the composition comprising statin comprises the steps of fermeting a substrate with a statin producing fungus and of extracting the substrate.|
FORM - 2
THE PATENTS ACT, 1970
(39 of 1970)
The Patents Rules, 2003
(See Section 10 and Rule 13)
COMPOSITION COMPRISING STATINS
HINDUSTAN LEVER LIMITED, a company incorporated under the Indian Companies Act, 1913 and having its registered office at Hindustan Lever House, 165/166, Backbay Reclamation, Mumbai -400 020, Maharashtra, India
The following specification particularly describes the invention and the manner in which it is to be performed
1 Composition comprising statins
5 Technical field
The present invention relates a composition comprising statin. The invention further relates to the use of the composition for the preparation of a food product and to a process for the preparation of the composition.
Background of the invention
Cardiovascular disease is a leading cause of morbidity and mortality, particularly in the United States and in Western European countries and is emerging in developing
15 countries. According to the American Heart Association, the major risk factors for heart disease are:
age (65 years and older), male gender, heredity, smoking, high blood-cholesterol levels (hypercholesteremia), high blood pressure, physical inactivity, diabetes, and obesity,
Obviously, some of these risk factors, such as age, gender, heredity, or diabetes, cannot be changed. However, it is possible to make lifestyle modifications which may decrease the risk of factors such as smoking, elevated blood-cholesterol,
25 elevated blood .pressure, physical inactivity, or obesity.
Elevated low-density lipoprotein cholesterol (hereafter "LDL
cholesterol") is directly related to an increased risk of coronary heart disease.
Statins are compounds that are known to have a lowering effect on levels of LDL-cholesterol in the human blood. Statins inhibit the hydroxymethylglutaryl coenzyme A (HMG-CoA)
reductase, the rate-determining step in the cholesterol biosynthesis.
Scientific research has confirmed the healthy properties of 5 statins especially with respect to LDL blood-cholesterol and triglyceride levels lowering activities, both in animals and in humans (Li et al., Nutrition Research 18, 71-81 (1998); Heber et al., Am. J. Clin. Nutr. 69, 231-236 (1999)).
10 The presence of statins in food consumed by humans is
associated with a lower level of LDL-cholesterol and lower risk of coronary heart disease.
People are considered obese when their BMI is over 30. The BMI 15 number is calculated as a person's weight divided by that
person's height. People with two or more risk factors for heart disease are at even higher risk for heart disease, when combined with a high BMI. In addition, obesity increases mortality of coronary heart disease 2-4 fold.
It is therefore imperative to have a healthy weight. Long term, modification in diet to achieve and maintain weight loss is essential. Bread, potatoes, pasta, rice fruit and vegetable are considered to be the basis of a healthy diet. It is generally
25 acknowledged that dietary fats should be kept to a minimum.
It would be beneficial to have a product that manages more than one risk-factors.
30 Compositions comprising statins for the manufacture of food products are known. WO02/64809 describes a process for the preparation of statins by fermentation and food products comprising one or more statins. It describes the extraction of
statins from fermented soya beans with organic solvents (ethanol and acetonitril) and the use of the extract for the preparation of magarine and spreads. The drawback of this process is that the yield of the statins is rather low (0.0545 5 g statin/kg (ethanol extract) and 0.0978 g statin /kg (acetonitril extract)).
In WO02/063976, fermented soy material comprising statin is used for the preparation of the food products. This fermented 10 soy material comprises about 20 wt.% oil. The high fat-content makes these kinds of statin containing material unsuitable for use in a low-fat diet.
Summary of invention
It is an object of the present invention to provide a composition comprising statins that can be used for the manufacture of food products. It is another object of the invention to provide a composition comprising statins that can
20 be used for the manufacture of low-fat food products.
Furthermore another object of the invention is to provide a low-fat food product comprising statins. Yet another object of the invention is to provide a process for the preparation of the composition comprising statins.
Surprisingly one or more of the above objects is obtained by a composition comprising statin wherein the composition is a flour comprising less than 10 wt.% of fat.
4 Detailed description of the invention
The present invention relates to a composition comprising statin characterised in that the composition is a flour
5 comprising less than 10 wt.% of fat. Preferably the flour
comprises less then 5 wt.% of fat, preferably less then 1 wt.% of fat or even more preferably essentially no fat.
For the purpose of this invention flour is defined as a fine
10 powder obtained by grinding a starchy plant material. Flour may be made from starchy plant materials including barley, buckwheat, chickpeas, lima beans, oats, peanuts, potatoes, soybeans, rice, and rye.
15 The composition according to the present invention comprises statins. Statins are defined as substances having the structural formula, presented in figure 1. In this structural formula, Rl and R2 can be any group. Preferred statins are those which are given in figure 1.
The amounts of statins given will be expressed, in wt.% or weight parts per million (ppm), mg/kg or mg/g, relative to the total weight of the food product, unless otherwise indicated.
25 The amounts of statins given herein are the sum of the amounts of individual statins, as e.g. determined by high performance liquid chromatography (HPLC) or LC-MS, unless otherwise indicated.
30 Preferably the composition according to the present invention comprises at least 0.01 mg/g statin, more preferably 0.1 mg/g statin. Even more preferred the flour of the invention comprises at least 1 mg/g statin.
One embodiment of the present invention relates to the use of the composition of the present invention in the preparation a food product.
Several food products may be prepared according to the
5 invention, for example, spreads, soups, pasta, noodles, ice cream sauces, dressing, snacks, cereals, beverages, bread, biscuits, muffins, other bakery products, sweets, bars, chocolate, dairy products, dietetic products such as slimming products or meal replacers etc. In particular bakery products
10 such as pancakes, muffins, and bread may be prepared according to the invention.
The food product according to the invention, in particular bakery products, has a low fat content. Preferably the food
15 product has less than 10 wt.% of fat, more preferably less than
The food product according to the invention preferably comprises statins in an amount sufficient to obtain a blood
20 LDL-cholesterol lowering effect if the food product is used according to the common needs of the consumer.
The preferred intake of statin per day is herein 5-40 mg/day, more preferably 5-20 mg/day, even more preferably 8-15 mg/day.
25 Furthermore, the intake of statin per day is preferably 1-5 mg/day, more preferably 1-2.5 mg/day.
The skilled person will be able to adjust the percentage of statins in the food product to obtain the desired blood
30 cholesterol lowering effect. The percentages will depend on the type of food product, since the food products are used in different serving sizes. Moreover the pattern in a food product
is consumed (servings per day and distribution over days) is dependent on the food product.
Preferably the food product comprises 0.1-10 mg statin per
5 serving, more preferably 1-5 mg statin per serving and even more preferably 1-2.5 mg statin, per serving.
Another embodiment of the present invention relates to a process for the preparation of composition comprising statin
10 wherein the composition is a flour comprising less than 10 wt.% of fat and the process comprises the steps of fermenting a substrate with a statin producing fungus, grounding the substrate, extracting the substrate, and recovering the extracted substrate, whereby the extracted substrate results in
15 a flour comprising statin with less than 10 wt.% of fat. The substrate can be grounded before or after the fermentation. Preferably the grounding is performed after the fermentation.
The fermented substrate may be extracted with any extracting
20 agent provided that the extracting agent extracts the fat from the substrate. For use in food applications, the extraction of fat from fermented soybeans can be performed with a number of organic solvents: hexane, acetone, ethyl acetate and ethanol or mixtures of these. Preferably the extracting agent extracts the
25 fat from the substrate so that the extracted substrates
comprises less than 10 wt.% fat, more preferably less than 5 wt.% fat, and even more preferably less than 1 wt.% fat. In addition, the preferred extracting agent leaves an effective amount of statin in the substrate. An effective amount of
30 statins stands for an amount sufficient to be effective in cholesterol-lowering. Preferably the extracted substrate comprises at least 0.01 mg statin per gram extracted substrate, more preferably at least 0.1 mg statin per gram extracted
substrate, even more preferred at least 1 mg statin per gram extracted substrate. Preferably the extracting agent is food grade. Hexane and super-critical CO2 are preferred extracting agents.
The substrate may vary depending on whether it may be fermented with a statin producing fungus. In addition, the substrates preferably have a high content of starchy material. Suitable substrates that may be used are soybeans, barley, buckwheat,
10 chickpeas, lima beans, oats, peanuts, potatoes, rice, and rye. Especially suitable substrate may be soybeans. Soybean flour may be used for a variety of products such as cookies, pastries, sweet goods, salty snacks e.g. nacho chips, prepared
15 meals, meat replacer, pasta, pancakes, muffins, bread and bread products. Furthermore, soybean flour comprises protein, fibers and phytoestrogen, saponins, polyphenols such as isoflavones, preferably in the non-glycosylated form. All of which have been shown to have a beneficial effect on the blood-cholesterol
20 level and the triglycerides level in the blood.
Soy protein content in a food product may be measured according to the method described in Gaiter et al., J. Sci. Food Agric. (1986), 37(3), 317-31.
Polyphenols include flavenoids, which include isoflavones. The polyphenols include isoflavones, stilbenes, lignans, coumestans and resorcyclic acid lactones. Examples of isoflavones are genistein, daidzein, equol, glycitein,
30 biochanin A, coumestrol, maitaresinol, formononetin, 0-
desmethylengolesin, enterolactone and enterodiol. Preferred isoflavones according to the invention are genistein and daidzein and glycitein, which are present in soybeans.
Saponins are herein derived as P-D-glucopyranosiduronic acid derivates. Examples of saponins are Soya sapogenol A,B,C,D and E, Soyasaponin I, II and III, as described in Lebensmittel
5 Lexikon, B.Behr's Verlag GmbH & Co. Hamburg, Bd.2, L-Z -3, 1993, pages 550-552.
Preferably the food product according to the invention comprises statin and non-glycosylated isoflavone. In soy beans
10 and soy materials derived from soy, isoflavones are present substantially in the glycosylated form. Typically about 5 wt.% of the isoflavones is present in the non-glycosylated form. The most important glycosylated isoflavones are genistin, daidzin and glycetin. The non-glycosylated forms are respectively
15 genistein, daidzein and glycetein. Genistein, daidzein and glycetein have been reported to have advantageous health effects, including estrogenic and antioxidant properties.
Due to the fermentation according to the invention the
20 glycosylated isoflavones are converted into the corresponding non-glycosylated isoflavones, which are more benificial. For instance, the amount of genistein and daidzein is increased in the fermented soy compared to the non-fermented soy.
25 The isoflavone concentration may be measured according to the HPLC method described in Franke A.A., et al. (1998): HPLC analysis of isoflavonoids and other phenolic agents from foods and human fluids; Proceed.Soc.Exp.Biol.Med; 217 (3), 274-280.
30 It has been shown that statins can be produced by a variety of filamentous fungi, including Monascus, Aspergillus, Penicillium, Pleurotus, Pythium, Hypomyces, Paelicilomyces, Eupenicillium, and Doratomyces.
Preferably the fungus is chosen from the group consisting of Monascus fungi and more preferably from the group consisting of Monascus ruber fungi.
5 As a food product, rice fermented with a red Monascus fungus (red rice) has been known and used for hundreds of years in China. Red rice was used and still is used in wine making, as a food-colouring agent and as drug in traditional Chinese medicine. Most red rice available on the market contains no
10 statins or statins in very low amounts. The Food and Drug
Administration has concluded that red yeast rice available in the market does not contain significant amounts of lovastatin (FDA, Docket No. 97-0441, Final Decision).
15 WO 99/23996 describes a composition for treating elevated serum cholesterol and/or triglycerides comprising a red rice product containing at least 0.05% lovastatin by weight.
Red rice powder capsules are sold as dietary supplements under
20 the name of Cholestin by the firm Pharmanex. Pharmanex also sells a Cholestin bar containing red yeast rice [Monascus purperus went) .
Red rice has an intensive red colour. Whereas the intensive red
25 colour of red rice is an advantage when it is used as colouring agent, it is a disadvantage when it is used in food products. Due to the intense red colour of red-rice products, the foods prepared from red rice are coloured, depending on the amount of red-rice product added to the food product yellow, orange or
30 red. The higher the amount of red rice added to the food, the more intense is the red colour of the food-product.
In some food products the red colouring is undesirable. In particular in the western world, consumers are reluctant to use products of which the colour has changed from that they are used to. For example bread, pancakes, muffins, spreads,
5 including margarine, butter, low fat spreads or salad oils are considered unacceptable by customers, when the colour of such a product is orange or red. However, at the same time these type of products have been found by us to be excellent vehicles of the daily intake of amounts of statins sufficient to obtain a
10 blood LDL-cholesterol lowering effect.
When colours are classified, they can be broken down into the three primary elements. One is the Hue (colour) the other is Value (brightness) and the third is Chroma (Saturation like
15 vivid colours or dull colours).
To enable anyone to tell anyone else exactly what colour they are talking about a common numerical code is used. This numerical code used is L*a*b*. When a colour is expressed in
20 this system, Value becomes L*, while Hue and Chroma are
expressed as a* and b* respectively. The L*a*b* may be measured with a UV 1601 spectofotometer of Shimatzu.
Preferably the food product has a Hue a* value of less than 20, 25 preferably less than 10, most preferably less than 0.
A preferred is Monascus strain is Monascus ruber F125 Ml-4. This strain when grown on soybeans doesn't have a red colouring.
Strain F125 Ml-4 is deposited at the Centraal Bureau voor Schimmelculturen (CBS) as no. CBS 109269 on 23.01.2001.
These deposits were made under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure and the Regulations thereunder (Budapest Treaty).
The fermentation may be carried out in a manner, which can be determined by the skilled person on the basis of the methods described in WO02/064809 and WO02/063976.
10 The fermentation temperature may be important. The temperature is preferably in the range of 10 to 37°C, more preferably 20 to 30°C.
Preferably during fermentation the medium is aerated, e.g. by 15 stirring, shaking etc. Aeration may be carried out by blowing air through the fermentation medium. Preferably the air is wholly or partly saturated with water vapour in case solid state fermentation is used. This avoids drying out of the fermentation medium.
The levels of statins will depend on the fermentation time. The fermentation time is therefore dependent on the desired amount of statins. Preferred fermentation time is 1-50 days, more preferably 15-40 days, most preferably 20-30 days (See 25 WO02/064809 and WO02/063976).
Description of the drawing
Fig. 1 shows a schematic representation of the structure of 30 different statins.
Fig. 2: shows a schematic representation of the experimental set-up for supercritical carbon dioxide extraction.
Determination of lovastatins using HPLC Samples were prepared by adding an extraction mixture,
5 containing acetonitrile, water and phosphoric acid (1:1:0.05, v/v/v) to the material in a ratio of 5:1. Usually 5 g of material was extracted with 25 ml of extraction mixture. Samples were incubated for 1 hour at room temperature and then homogenised using an Ulta-Turrax. After homogenisation the
10 samples were incubated overnight at room temperature on a
roller bench. The samples were centrifuged at 11.000 rpm for 10 minutes and the supernatants collected for HPLC analysis. The amount of lovastatin in the samples was determined by HPLC separation according to the method of Morovjan et al. J.
15 chromatogr. A 763 (1997) 165-172.
The system consists of the Shimadzu SCL-10A system controller, CTO-10AS column oven, LC-10AT vp pump system, RID-10A refraction index detector, SPD-M10A diode array detector and SIL-10AD autoinjector. For the chromatographic determination of
20 lovastatin a Waters NovaPak C18 (150x3.9 mm I.D., 4^im) column was used operating at 25°C. The eluent was an acetonitrile, water and phosphoric acid (1:1:0.05 v/v/v) solution flowing at 1.5 ml/min. Runs were performed for 15 min. The detection was performed using a diode array detector from 190 nm up to 800
25 nm. The sum of the area of all peaks in the spectrum belonging to lovastatin is measured. Comparison to a standard (Mevinolin, Sigma) allows the calculation of a lovastatin content (expressed in mg/g analysed product).
WO 2005/104871 PCT/EP2005/003247
13 Example 1-8: Preparation of fermented substrates
Monascus ruber F125 Ml-4 (CBS 109269) was plated on VMA-agar
5 plates and incubated at 30 °C for 3 days.
With a sterile scalpel, small squares were cut in the VMA-agar for the preparation of inoculates. With a sterile spatula, the blocks of agar were transferred to the liquid media. Malt water was used for pre-cultivation. Sterile flasks of 500 ml were
10 filled with 300 ml medium. The flasks were incubated in an Innova 400 shaker at 25°C for 2 days.
Substrates like soybeans, kidney bean, mung bean, lupine seeds, walnut, maize, oat and peanuts were soaked in tap water (50°C)
15 for 60 minutes. After soaking the substrates were rinsed with cold tap water. Subsequently the substrates were air-dried at ambient temperature for 180 minutes. The soaked and dried substrates were transferred to a shake-flask, approximately 50 g per flask. The shake flasks were sterilised by autoclaving
20 (10 min at 120°C), inoculated with 1 ml of a fully-grown Monascus culture and incubated for an appropriate time (2-6 weeks) at 25°C. Lovastatin production is monitored and when a sufficient level has been obtained, the flasks are pasteurised by placing the flasks in an incubator at 80°C overnight after
25 which the end product can be harvested. Table 1 gives an
overview of the statin content of the end product after 3 weeks of fermentation.
Table 1: Amount of statin in end product after 3 weeks of fermentation
Example Substrate Statin/g/kg
1 Soy beans 1.2
2 Kidney beans 1.2
3 Mung beans 1.2
4 Lupine seeds 1.5
5 peanut 0.4
6 Walnut 0.8
7 Maize 0.3
8 Oat 1.4
Example 9: Supercritical extraction of fermented soybeans
5 For the supercritical extraction of natural solid matrices, equipment and software of Thar Designs, USA was used. The experimental set-up is schematically depicted in Fig. 2. The C02 pump is capable of compressing liquid carbon dioxide to a pressure up to 600 bars at a constant flow-rate. In a static
10 mixer,, a polarity modifier may be mixed with the liquid carbon dioxide. In a pre-heater (not depicted) the carbon dioxide was heated to reach supercritical conditions before entering the extraction vessel.
15 In the extraction vessel, which was heated with a double wall heating mantle, the supercritical carbon dioxide was passed over the solid matrix for extraction.
Downstream of the extraction vessel, the supercritical carbon
20 dioxide was expanded over an automated backpressure regulator. The backpressure regulator was coupled to a feedback control unit to control the pressure in the system.' The carbon dioxide was separated from the extracted material(liquid/solid) in a cyclone separation system. The carbon dioxide left the cyclone
at the top, while the extracted material remained in the cyclone. The liquids extracted from the solid matrix were recovered during the experiment by opening the valve at the bottom of the cyclone.
The carbon dioxide gas was further expanded over a further backpressure regulator, which was operated manually. A gas clock downstream of the backpressure regulator registers how much gas has been put through the system, before the carbon
10 dioxide leaves the system at ambient pressure.
The process equipment is designed to operate at the following conditions:
Table 2: Process operation conditions supercritical extraction
Flow rate of liquid carbondioxide 5 - 150 g/min
Pressure up to 600 bar
Temperature 20 -95°C
Extractor volume 500 mL
Cyclone volume 200 mL
Matrix to be extracted solid
The fermented soybeans were ground prior to extraction in a
5 water-cooled universal mill (type M20, IKA, Germany) until a fine powder was obtained.
The amount of sample used for extraction was 100 gram of ground fermented soybeans. The fermented and ground soybeans were put
10 in the extraction vessel and the remaining volume was filled with small glass beads (2 mm diameter). The flow rate of liquid carbondioxide was 20 g-min""1. In the attached cyclone separation vessel, the extracted oil was collected. The total extraction time was 2 hours.
15 After the extraction, the extraction vessel was opened and a dry free-flowing soybean flour powder was obtained. The added glass beads were removed by sieving. An aliquot of the soybean flour was analysed for the amount of Lovastatin. Both in the starting material as well as in the extracted material the
20 amount protein, moisture fat and ash were determined. An example of the analytical results is presented in Table 3.
17' Table 3: Composition of grounded fermented soybeans before and
after supercritical fluid extraction.
Before extraction After extraction
Protein (wt.% ) 43.5 51.6
Fat (wt.% ) 19.4 3.3
Moisture (wt.% ) 6.0 3.6
Ash (wt.% ) 6.0 6.9
Lovastatin (mg / g) 0.61 1.04
Example 10: Soxhlet extraction with hexane of fermented 5 soybeans
Comperative example A: Soxhlet extraction with ethanol of fermented soybeans
150 g of grounded fermented soybeans was put in an extraction
10 thimble. 800 ml of organic solvent was added and the extraction was performed for 6 hours. After the extraction, the extraction thimble was taken out of the extraction equipment. The extraction thimble was emptied and the obtained soy powder was dried on air until constant weight. An aliquot was taken for
15 the determination of the lovastatin concentration. Both in the starting material as well as in the extracted material the amount of ash, protein fat and moisture were determined. The analytical results are shown in Table 4.
20 Table 4: Result of Soxhlet extraction of Monascus fermented
Grounded fermented soybeans
Before Extraction After extrction
Ex. 10 Comp.Ex. A
Solvents Hexane Ethanol
Protein (wt.% ) 43.4 55.2 45.4
Fat (wt.% ) 17.4 4.0 ' 14.4
Moisture (wt.% ) 1.6 2.9 5.8
Ash (wt.% ) 5.7 7.8 6.6
Lovastatin (mg / g) 0.94 1.42 0.66
Both with supercritical fluid extraction as well as with hexane extraction soybean fat is efficiently removed resulting in a defatted soy flour(fat content 5 defatted soy flour. Ethanol is less suitable as it does not remove the fat as efficient as hexane or supercritical fluid and also leaves less statin in the extracted flour.
Example 11: Preparation of low-fat oat-pancakes with banana and 10 orange
Serving size: 65 g Formulation:
Ingredient (wt.% )
Rolled oats 6.3
Unbleached flour 8.0
soy flour with 1 mg/g statins, 4% fat 4
Baking powder 1.7
Plain soy milk 45
Banana (thinly sliced) 16
Orange (thinly sliced) 19 .
15 In a large bowl, combine the rolled oats, unbleached flour, soy
flour and baking powder. Add the soymilk, and blend with a few
swift strokes. Fold in the banana and orange slices.
Pour 0.25 cup (65 g) of the batter onto a hot non-sticky
griddle or pan. Cook for about 2 min or until bubbles appear at 20 the surface. Flip the pancake and cook for another minute or
until heated through.
The pancakes can be served with e.g. maple 'syrup, apple sauce
or a fruit spread.
Serving size 65 g, per serving: 2.6 mg of statins,
Example 12: Preparation of low-fat raisin bread Formulation:
Bread flour 42.5
soy flour with 1 mg/g statins, 4% fat 5.4
Active dried yeast 1.4
Plain soy milk 34
Orange (skins) (dried and rehydrated with sugar syrup) 4
5 Mix the ingredients thoroughly in a dough mixer. Proof the dough. Allow the dough to rise for 30-60 min at a temperature of 20°C. Put the risen dough in a bread tin. Bake the bread at 190°C for 30-60 min.
Serving size: 40 g, amount of statins per serving 2.2 mg. 10
WO 2005/104871 PCT/EP2005/003247
Example 13: Preparation of low-fat orange-banana muffins formulation:
Engredient (wt.% )
soy flour with 1 mg/g statins, 4% fat" 8.7-
All purpose flour 8.9
Whole wheat flour 8.6
Baking soda 0.7
All spice powder 0.5
Dried and rehydrated orange skin cubes 5.4
Orange juice concentrate 3.6
Egg whites 14.3
5 Combine dry ingredients in bowl. Mix orange juice, vanilla,
banana, honey and half of the egg whites in a bowl on low speed
until blended. Add dry ingredients and mix for a few seconds,
just until moistened and no flour can be detected.
Beat the remaining egg whites and stir into the above mixture. 10 Add raisins. Fill paper cupcake liners in muffin tins and fill
cups to within 0.3 inch of top. Bake at 180°C for 10-24 min
until you can touch the muffin lightly on top and the imprint
does not remain.
Serving size: 75 g, amount statins per serving: 6.5 mg.
15 The flour with statins may be party replaced by whole wheat
flour and/or all purpose flour, this allows for easy variation
of the statin content.
WO 2005/104871 PCT/EP2005/003247
Example 14: Preparation of low-fat pasta
Ingredient (wt.% )
Ibis Flour (NBS/14%PROT) 74.65
Tapioca starch pre cooked 20% (Nat. Starch) 8.50
Palm oil 1.13
soy flour with 1 mg/g statins, 4% fat 1.13
Natrium Alginate -Manucol Dmb(Kelco) 0.06
Titanium dioxide 0.01
The ingredients were mixed to form the pasta. The pasta was then formed with a pasta maker into long noodle-like threads.
5 The pasta was cooked for 5 minutes. Serving size 80 g, amount statins per serving: 1 mg. The consumption of 80 g pasta would result in an estimated blood cholesterol lowering of 5%.
1. Composition comprising statin characterised in that the composition is flour comprising less than 10 wt.% of fat.
2. Composition according to claim 1 comprising less than 5 wt.% of fat.
3. Composition according to claim 2 comprising less than 1 wt.% of fat.
4. Composition according to any of claims 1 to 3 comprising at least 0.01 mg/g statin.
5. Composition according to claim 4 comprising at least 0.1 mg/g statin.
6. Composition according to claim 5 comprising at least 1 mg/g statin.
7. Use of a composition according to any of claims 1 to 6 in the preparation a food product.
8. Use according to claim 7 wherein the food product is a bakery product.
9. A food product comprising statin characterised in that it comprises less than 10 wt.% of fat.
10. A food product according to claim 9 having a Hue a* value of less than 20, preferably less than 10, most preferably less than 0.
11. A food product according to claim 9 or 10 selected from the group consisting of muffins, bread, pancake, and pasta.
12. Process for the preparation of composition comprising statin wherein the composition is a flour comprising less than 10 wt.% of fat, and wherein the process comprises the steps of
fermenting a substrate with a statin producing fungus,
grounding the substrate,
extracting the substrate, and
recovering the extracted substrate,
whereby the extracted substrate results in a flour
comprising statin with less than 10 wt.% of fat.
13. Process according to claim 12 wherein the substrate is extracted with hexane or super-critical C02.
14. Process according to claim 12 or 13 wherein the substrate are soybeans.
15. Process according to any of the claims 12 to 14 wherein the statin producing fungus is a Monascus fungus.
Dated this 27th day of October 2006
HINDUSTAN LEVER LIMITED
(S. Venkatramani) Senior Patents Manager
|Indian Patent Application Number||1261/MUMNP/2006|
|PG Journal Number||07/2009|
|Date of Filing||27-Oct-2006|
|Name of Patentee||HINDUSTAN UNILEVER LIMITED|
|Applicant Address||HINDUSTAN LEVER HOUSE, 165-166 BACKBAY RECLAMATION, MUMBAI 400020, MAHARASHTRA,INDIA.|
|PCT International Classification Number||A23L1/20|
|PCT International Application Number||PCT/EP2005/003247|
|PCT International Filing date||2005-03-23|