Indian Patents. 225465:ANTIBACTERIAL HETEROBICYCLIC SUBSTITUTED PHENYL OXAZOLIDINONES COMPOUNDS

Title of Invention	ANTIBACTERIAL HETEROBICYCLIC SUBSTITUTED PHENYL OXAZOLIDINONES COMPOUNDS
Abstract	Bicycllc heterocyclic substituted phenyl oxazoildinone compounds of formula (I): wherein Y is a radical of formulae (II) or (III) In which the substituents have the meaning indicated in the description. These compounds are useful as antibacterial agents.

Full Text	ANTIBACTERIAL HETEROBICYCLIC SUBSTITUTED PHENYL OXAZOLIDINONES FIELD OF THE INVENTION The present invention relates to the field of phenyl oxazolidinone compounds having antibacterial activity against Gram-positive and Gram- negative bacteria, pharmaceutical compositions containing the compounds, and methods of treating bacterial infections with the compounds. BACKGROUND OF THE INVENTION Oxazolidinones have been identified, within the last twenty years, as a new class of antibacterials which are active against numerous multidrug- resistant gram positive organisms. Particularly problematic pathogens include methicillin-resistant Staphylococcus aureus (MRSA), glycopeptide- intermediate resistant Staphylococcus aureus (GISA), vancomycin-resistant enterocci (VRE) and penicillin- and cephalosporin-resistant Streptococcus pneumoniae. As a class, oxazolidinones exhibit a unique mechanism of action. Studies have shown that these compounds selectively bind to the 50S ribosomal subunit and inhibit bacterial translation at the initiation phase of protein synthesis. Exemplary members of oxazolidinones are linezolid (see WO 95/07271) and eperezolid. U.S. Pat. No. 5,792,765 to Riedi et al. discloses a series of substituted oxazolidinones (cyanoguanidine, cyanoamidines, and amidines) useful as antibacterial medicaments. U. S. Patent No. 5,910,504 to Hutchinson discloses a series of hetero- aromatic ring substituted phenyl oxazolidinones, including indolyl substituted compounds useful as antibacterial agents. WO 98/54161 (Hester et al.) discloses amides, thioarnides, ureas, and thioureas which are antibacterial agents. WO 95/07271 (Barbachyn et al.) discloses oxazine and thiazine oxazolidinone derivatives such as linezolid and its analogs which are useful antimicrobial agents, effective against a number of human and veterinary pathogens, including gram-positive aerobic bacteria such as multiple-resistant staphylococci, streptococci and enterococci as well as anaerobic organisms such as Bacteroides spp. and Clostridia spp. species, and acid-fast organisms such as Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium spp. WO 93/09103 (Barbachyn et al.) discloses substituted aryl- and heteroarylphenyloxazolidinones which are useful as antibacterial agents. SUMMARY OF THE INVENTION The invention provides phenyl oxazolidinone compounds of Formula I: wherein: R is selected from the group consisting of OH, N3, OR', O-Aryl, O-Heteroaryl OSO2R", -NR"R", or (i) R' is straight-chain or branched acyl having up to 6 carbon atoms or benzyl; (ii) R" is straight-chain or branched alky!, having up to 5 carbon atoms, phenyl or tolyl; and (iii) R'" and R"" are independently selected from the group consisting of H, cycloalkyl having 3 to 6 carbon atoms, phenyl or tert-butoxycarbonyl, fluorenyioxycarbonyl, benzyloxycarbonyl, straight-chain or branched alkyl having up to 6 carbon atoms which is optionally substituted by cyano or alkoxycarbonyf having up to 4 carbon atoms, -CO2-R1, -CO-R1, -CO-SR1, - CS-R1, P(O)(OR2)(OR3), and -SO2-R4, in which R1 is selected from the group consisting of H, cycloalkyl having 3 to 6 carbon atoms, trifluoromethyl or phenyl, benzyl, or acyl each having up to 5 carbon atoms, straight-chain or branched alkyl having up to 6 carbon atoms, said alkyl optionally substituted by straight-chain or branched alkoxycarbonyl having up to 5 carbon atoms, OH, cyano, up to 3 halogen atoms, and -NR5 R6 in which R5 and R6 are identical or different and are selected from H, phenyl or straight-chain or branched alkyl having up to 4 carbon atoms; R2 and R3 are identical or different and are selected from hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms; and R4 is selected from straight-chain or branched alkyl having up to 4 carbon atoms or phenyl and; R4a is CN, COR4C, COOR4C, CONHR4C CO-NR4C R4d, SO2R4C. SO2NHR4c, SO2-NR4C R4C, or NO2; R4b is H, alkyl, OR4c, SR4c, amino, NHR4c, NR4c,R4c, C1-C8-alkylaryl or mono-, di-, tri-, and per-halo C1-C8-alkyl; R4c and R4d are independently selected from H, alkyl, aryl, or in the case of any NR4CR4C group R4C and R4d taken together with the nitrogen atom to which they are attached form a unsubstituted or substituted pyrrolidinyl, piperidinyl or morpholinyl group; X is 0 to 4 members independently selected from the group consisting of halogen, OH, mercapto, nitro, halo-C1-8-alkyl, C1-8 alkoxy, thio-C-1-8-alkyl, C1-8 alkyl-amino, di(C1-8-alkyl-)amino, formyl, carboxy, alkoxycarbonyl, C1-8 alkyl- CO-O-, C1-8 alkyl-CO-NH-, carboxamide, aryl, substituted-aryl, heteroaryl, substituted-heteroaryl, CN, amine, C3-6 cycloalkyl, C1-8 alkyl optionally substituted with one or more members selected from the group consisting of F, CI, OH, C1-8 alkoxyl and C1-8 acyloxy; and Y is a radical of Formulae II or III: wherein R5, R6, R7, and R8 are each independently H, alkyl, CN, nitro, C1-8 alkyl, halo-C1-8-alkyl, formyl, carboxy, alkoxycarbonyl, carboxamide, aryl, substituted-aryl, heteroaryl, substituted-heteroaryl, or R5 and R6 and/or R7 and R8 together form an oxo group; R9, and R10 are each independently H, halogen, alkyl, OH, CN, mercapto, nitro, C1-8 alkyl, halo-C1-8-alkyl, C1-8 alkoxy, thio-C1-8-alkyl, amino, C1-8- alkyl-amino, di(C1-8-alkyl-)amino, formyl, carboxy, alkoxycarbonyl, C1-8- alkyl-CO-O-, C1-8-alkyl-CO-NH-, carboxamide, aryl, substituted-aryl, heteroaryl, substituted-heteroaryl, or amine; Rg, and R10 are each independently H, halogen, alkyl, OH, CN, mercapto, nitro, C1.8 alkyl, halo-d-e-aikyl, Ci.8 alkoxy, thio-d-s-alkyl, amino, d-8- alkyl-amino, di(Ci.e-alkyl-)amino, formyl, carboxy, alkoxycarbonyl, C1-8- alkyl-CO-O-, d-e-alkyl-CO-NH-, carboxamide, aryl, substituted-aryl, heteroaryl, substituted-heteroaryl, or amine; A, B, C, and D are selected from C, S, O, and N to form any five to ten membered aromatic or heteroaromatic ring, said heteroaromatic ring having one to four members selected from the group consisting of S, O, and N; Z is selected from halogen, alkyl, aryl, substituted-aryl, heiteroaryl, substituted-heteroaryl, CN, CHO, COalkyl, amine, (dialkylamino)alkyl, said dialkylamino consisting of straight-chain or branched alkyl having up to 6 carbon atoms or phenyl or constituting a ring of 2 to 5 carbons having 0 to 2 atoms selected from S, O and N or alkoxy, or NHCO-(C1-C8-alkyl); and m is 0 or 1, and the pharmaceutically acceptable salts and esters thereof. Compounds of the above formula are useful as antibacterial agents for the treatment of bacterial infections in humans and animals. The present invention is also directed to a method of treating a subject having a condition caused by or contributed to by bacterial infection, which comprises administering to said mammal a therapeutically effective amount of the compound of Formula I. The present invention is further directed to a method of preventing a subject from suffering from a condition caused by or contributed to by bacterial infection, which comprises administering to the subject a prophylactically effective dose of the pharmaceutical composition of a compound of Formula I. Other objects and advantages will become apparent to those skilled in the art from a review of the ensuing specification. prophylactically effective dose of the pharmaceutical composition of a compound of Formula I. Other objects and advantages will become apparent to those skilled in the art from a review of the ensuing specification. DETAILED DESCRIPTION Relative to the above description of the phenyl oxazolidinone compounds of the present invention, the following definitions apply. Unless specified otherwise, the terms "alkyl", "alkenyl", and "alkynyl" may be straight or branched groups with 1-8 carbon atoms. "Acyl" means an organic radical having the designated number of carbon atoms, derived from an organic acid by the removal of a hydroxyl group having the formula RCO, as in the case of acetyl where R is CH3. "Aryl" is an unsubstituted carbocyclic aromatic group including, but not limited to, phenyl, 1- or 2-naphthyl and the like. "Heteroaryl" refers to a cyclic aromatic radical having from five to ten atoms in the ring; where one to three ring atoms are independent heteroatoms such as S, O, and N, and the remaining ring atoms are carbon, for example, a pyridinyl, pyrazinyl, pyrimidinyl, pyrroyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isoxazolyl, thiadiazolyl, oxadiazolyt, thienyl, furanyl, quinolinyl, or isoquinolinyl, radical and the like. "Substituted aryl" or "substituted heteroaryl" refers to an aryl or heteroaryl substituted by independent replacement of 1-3 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, C1-8-alkyl, halo-C-1-8- alkyl, C-1-8-alkoxy, thio-C1-8-alkyl, amino, C1-8-alkyl-amine, di(C1-C8-alkyl- )amino, formyl, carboxy, alkoxycarbonyl, C1-8-alkyl-CO-O-, C1-8-alkyl-CO-NH-, or carboxamide. Further, substituted-heteroaryl may be substituted with a mono-oxo to give, for example, a 4-oxo-1-H-quinoline. Substituted-heteroaryl may also be substituted with a substituted-aryl or a second substituted- heteroaryl to give, for example, a 4-phenyi-imidazol-1-yl or a 3-pyridinyl- imidazol-1-yl, and the like. The term "halo" or "halogen" means fluoro, chloro, bromo and iodo. (mono-, di-, tri-, and per-) halo-alkyl is an alkyl radical substituted by independent replacement of the hydrogen atoms thereon with halogen. P denotes phosphorus. The compounds of the instant invention are asymmetric in the oxazolidinone ring at the 5- position and thus exist as optical antipodes. As such, ail possible optical antipodes, enantiomers or diastereomers resulting from additional asymmetric centers that may exist in optical antipodes, racemates and racemic mixtures thereof are also part of this invention. The antipodes can be separated by methods known to those skilled in the art such as, for example, fractional recrystallization of diastereomeric salts of enantiomerically pure acids. Alternatively, the antipodes can be separated by chromatography on a Pirkle column. The phrase "pharmaceutically acceptable salts" denotes salts of the free base which possess the desired pharmacological activity of the free base and which are neither biologically nor otherwise undesirable. These salts may be derived from inorganic or organic acids. Examples of inorganic acids are hydrochloric acid, nitric acid, hydrobromic acid, sulfuric acid, or phosphoric acid. Examples of organic acids are acetic acid, propionic acid, glycolic acid, lactic acid, pyruvic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, rnandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, methyi sulfonic acid, saiicyclic acid and the like. Suitable salts are furthermore those of inorganic or organic bases, such as KOH, NaOH, Ca(OH)2, AI(OH)3, piperidine, morpholine, ethylamine, triethylamine and the like. Also included within the scope of the invention are the hydrated forms of the compounds which contain various amounts of water, for instance, the hydrate, hemihydrate and sesquihydrate forms. The term "subject" includes, without limitation, any animal or artificially modified animal. In the preferred embodiment, the subject is a human. The term "drug-resistant" or "drug-resistance" refers to the characteristics of a microbe to survive in presence of a currently available antimicrobial agent at its routine, effective concentration. The compounds of the present invention possess antibacterial activity against Gram-positive and certain Gram-negative bacteria. They are useful as antibacterial agents for the treatment of bacterial infections in humans and animals. Particularly, these compounds have antimicrobial activity against S. aureus, S. epidermidis, S. pneumoniae, E. faecalis, E. faecium, Moraxelia catarrhalis, and H. influenzae. More particularly, these compounds are useful against resistant bacteria such as MRSA and GISA, and have a low susceptibility to acquired resistance mechanisms. Compounds of Formula I which are most preferred for such purposes are those in which R is any of the following: In addition Compounds of Formula I which are most preferred for such purposes are those in which Y is any of the following: Particular examples of the present invention include the following compounds: N-[[(5S)-3-[4-(1,3-Dihydro-2H-isoindol-2-yl)-3-fluorophenyl]-2-oxo-5- oxazolidinyl]methyl]acetamide; N-[[(5S)-3-[4-(1,3-Dihydro-pyrrolo[3,4-c]pyridin-2-yl)-3- fluorophenyl]-2-oxo-5-oxazolidinyl]methyl]acetamide; N-[[(5S)-3-[3-Fluoro-4-(5-oxido-2H-pyrrolo[3,4-clpyridin-2-yl)phenyl]-2- oxo-5-oxazolidinyl]methyl]acetamide; N-[[(5S)-3-[4-(5,7-dihydro-6H-pyrrolo[3.4-b]pyridin-6-yl)-3-fluorophenyl]- 2-oxo-5-oxazolidinyl]methyl]acetamide; N-[(5S)-3-[4-(1,3-dihydro-1-oxo-2H-isoindol-2-yl)-3-fluorophenyl]-2- oxo-5-oxazolidinyl]methyl]acetamide; and (5R)-3-[4-(5,7-Dihydro-6H-pyrrolo[3,4-b]pyridin-6-yl)-3-fluorophenyl]- 5-(hydroxymethyl)-2-oxazolidinone. The compounds of Formula I that are the subject of this invention may be prepared from readily available starting materials such as isoindole (Gawley et al., J. Org. Chem., 1988, 53:5381), 6,7-dihydro-5H-pyrrolo[3,4-c]pyridine and 6,7-dihydro-5H-pyrrolo[3,4-b]pyridine (US Pat. No. 5,371,090 to Petersen et al.) in accordance with synthetic methods well known in the art. Representative proceduresare outlined in Scheme I-V: In accordance with Scheme I, bicyclic heterocycles of general formula IV are treated with a substituted nitrobenzene derivative (L is an appropriate leaving group such as a halogen of trifluoromethanesulfonyloxy) in a suitable base and solvent, such as diisopropylamine and ethyl acetate, to give the substituted nitrophenyl compound V. The nitrobenzene derivative V is then reduced to the aniline by an appropriate reaction, for instance by treatment with SnCl2 or by catalytic hydrogenation in the presence of a suitable catalyst, such as palladium on carbon. The aniline is then treated with benzyl or methyl chloroformate and sodium bicarbonate to form the corresponding benzyl or methyl carbamate derivative VI. The Cbz aniline VI is then deprotonated with a lithium base such as n- butyllithium and reacted with (R)- glycidyl butyrate to afford the oxazolidinone VII. The hydroxymethyl group can then be converted to an amide as shown in Scheme I by preparation of the mesylate, conversion to azide VIII, and reduction to amine IX by an appropriate procedure such as hydrogenation. Alternatively displacement of a mesylate (Scheme II) or appropiate leaving group such as tosylate or chlorine with potassium phthalimide and removal of the phthaloyl protecting group by hydrazinolysis would provide amine IX. The amine IX can be converted to amide X by an acylation reaction using techniques known in the art, such as treatment with acetic anhydride in the presence of a base such as pyridine. Alternatively, amine IX can be converted to a carbamate XI by treatment with methylchloroformate and pyridine, or reacted with a sulfonyl chloride in an inert solvent in the presence of an organic base like pyridine to form a sulfonamide XII For the formation of oxazolidione in which R = O-Heteroaryl (XIII), the oxazolidinone carbinol VII can be converted to the corresponding mesylate or other appropriate leaving group and reacted with HO-Het (a suitible hydroxyl containing heterocycle), either in the presence of base or with HO-Het as a preformed alkoxide, in an appropriate solvent, for example DMF or acetonitrile (Scheme III). Alternatively, Mitsunobu conditions can be used to couple VII with HO-Heterocycle by treating with triphenylphosphine and diisopropyl azodicarboxylate (DIAD) in an appropriate solvent, such as THF, at a suitable temperature, preferably room temperature. Reaction conditions and leading references can be found in Gravestock et at, WO99/64416. Furthermore, by treating VII with a suitable, non-nucleophilic base, for example NaH, the displacement of a leaving group (LG), such as chlorine or bromine, can be effected from an appropriately reactive aza-heterocycle (LG- Het)(Scheme III). Compounds of structure XIV can be prepared as shown in Scheme IV. Amine IX can be converted to various functionalized amidines by reaction with activated imines, where Q is a leaving group such as methylthio or methoxy, in a suitable solvent, for example toluene or methanol, with or without a catalyst (such AgNO3) present at a temperature range of 0-110ºC. In accordance with Scheme V pyrrolidinone XV (prepared as in WO96/13502) is first reacted with methoxy-bis(dimethylamine) or other activated dimethylformamide reagent and, second, heated in a suitable solvent (for example DMF and benzene) with either substituted amidines, to form pyrrolopyrimidines oxazolidinones such as XVI, or substituted hydrazines, to form pyrrolopyrazole oxazolidinones such as XVII. Formation of the enamine, alkoxymethylene or alkoxycarbonyl derivatives of pyrrolidinone XV, according to Brighty et al in US 5037834A, would also allow access to these systems. Scheme VI As shown in Scheme VI compounds with the structure XIX can be achieved by oxidation of the various compounds, XVIII, using an appropriate oxidant (for example manganese dioxide, peroxyacetic acid, DDQ or air) in a suitable solvent such as methylene chloride. Oxo-derivatives of structure XXII in Scheme VII, (X = O, Y = H2 or X = H2, Y = O) can be constructed by reacting 1,2-aryl dicarboxaldehydes (where XXI, U = H) with aniline XX (prepared as in WO96/23788) in the presence of acids, such as acetic acid, in a suitable solvent such as methylene chloride. The di-oxo-derivatives ( structure XXII where X = Y = O) are prepared from the reaction of aniline XX with selected 1,2-aryl dicarbonyl reagents with a suitable leaving group (XXI where U = CI, Br, etc). Definitions All temperatures are in degrees Centigrade Brine refers to an aqueous saturated sodium chloride solution DMF refers to N,N-dimethylformamide THF refers to tetrahydrofuran Cbz refers to carbobenzyloxy n-BuLi refers to n-butyl lithium MS refers to mass spectrometry expressed as m/e or mass/charge unit [M + H] refers to the positive ion of a parent plus a hydrogen atom Ether refers to diethyl ether rt refers to room temperature Mp refers to melting point CH2CI2 refers to methylene chloride NaOH refers to sodium hydroxide MeOH refers to methanol EtOAc refers to ethyl acetate ppt refers to a precipitate These compounds have antimicrobial activity against susceptible and drug resistant bacterial pathogens such as S. aureus, S. epidermidis, S. pneumoniae, S. pyogenes, Enterococcus spp., Moraxella catarrhalis and H. influenzae. These compounds are particularly useful against drug resistant Gram-positive cocci such as methicillin-resistant S. aureus and vancomycin- resistant enterococci. These compounds are useful in the treatment of community-acquired pneumonia, upper and lower respiratory tract infections, skin and soft tissue infections, hospital-acquired lung infections, bone and joint infections, and other bacterial infections. Minimal inhibitory concentration (MIC) has been an indicator of in vitro antibacterial activity widely used in the art. The in vitro antimicrobial activity of the compounds was determined by the microdilution broth method following the test method from the National Committee for Laboratory Standards (NCCLS). This method is described in the NCCLS Document M7-A4, Vol.17, N0.2, "Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically-Fourth Edition", which is incorporated herein by reference. In this method twofold serial dilutions of drug in cation adjusted Mueller-Hinton broth are added to wells in microdilution trays. The test organisms are prepared by adjusting the turbidity of actively growing broth cultures so that the final concentration of test organism after it is added to the wells is approximately 5x104 CFU/well. Following inoculation of the microdilution trays, the trays are incubated at 35ºC for 16-20 hours and then read. The MIC is the lowest concentration of test compound that completely inhibits growth of the test organism. The amount of growth in the wells containing the test compound is compared with the amount of growth in the growth-control wells (no test compound) used in each tray. As set forth in Table 1, some compounds of the present invention were tested against a variety of pathogenic bacteria resulting in a range of activities, from 1 to >128 µg/mL depending on the organism tested. S. aureus OC2878 is a MRSA and E. faecium OC3312 is a vancomycin resistant enterococcus. This invention further provides a method of treating bacterial infections, or enhancing or potentiating the activity of other antibacterial agents, in a subject having conditions caused by or contributed to by bacterial infection, which comprises administering to the animals a compound of the invention alone or in admixture with another antibacterial agent in the form of a medicament according to the invention. The terms of "treating" and "treatment" include administering, either simultaneously, separately or sequentially, a pharmaceutically effective amount of a composition containing one or more of the compounds disclosed herein to a subject that desires inhibition of bacterial growth. The pharmaceutically effective amount of the compound used to practice the present invention for treatment varies depending on the manner of administration, the age, weight, and general health of the subject treated, and ultimately will be decided by physicians or veterinarians. The compounds of the present invention may be administered to a subject such as a human by any route appropriate to the condition to be treated, suitable routes including oral, rectal, nasal, topical (including buccal and sublingual), vaginal and parenteral (including subcutaneous, intramuscular, intravenous, intradermal, intrathecal and epidural). The preferred route may vary with, for example, the condition of the recipient as well as the ease of preparation and administration. When the compounds are employed for the above utility, they may be combined with one or more pharmaceutically acceptable carriers, e.g., solvents, diluents, and the like, and may be administered orally in such forms as tablets, capsules, dispersible powders, granules, or suspensions containing for example, from about 0.5% to 5% of suspending agent, syrups containing, for example, from about 10% to 50% of sugar, and elixirs containing, for example, from about 20% to 50% ethanol, and the like, or parenterally in the form of sterile injectable solutions or suspensions containing from about 0.5% to 5% suspending agent in an isotonic medium. These pharmaceutical preparations may contain, for example, from about 0.5% up to about 90% of the active ingredient in combination with the carrier, more usually between 5% and 60% by weight. Compositions for topical application may take the form of liquids, creams or gels, containing a therapeutically effective concentration of a compound of the invention admixed with a dermatologically acceptable carrier. In preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed. Solid carriers include starch, lactose, dicalcium phosphate, microcrystalline cellulose, sucrose and kaolin, while liquid carriers include sterile water, polyethylene glycols, non-ionic surfactants and edible oils such as corn, peanut and sesame oils, as are appropriate to the nature of the active ingredient and the particular form of administration desired. Adjuvants customarily employed in the preparation of pharmaceutical compositions may be advantageously included, such as flavoring agents, coloring agents, preserving agents, and antioxidants, for example, vitamin E, ascorbic acid, BHT and BHA. The preferred pharmaceutical compositions from the standpoint of ease of preparation and administration are solid compositions, particularly tablets and hard-filled or liquid-filled capsules. Oral administration of the compounds is preferred. These active compounds may also be administered parenterally or intraperitoneally. Solutions or suspensions of these active compounds as a free base or pharmacological acceptable salt can be prepared in water suitably mixed with a surfactant such as hydroxypropyl- cellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparations may contain a preservative to prevent the growth of microorganisms. The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils. The effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration and the severity of the condition being treated. However, in general, satisfactory results are obtained when the compounds of the invention are administered at a daily dosage of from about 0.1 mg/kg to about 400 mg/kg of animal body weight, preferably given in divided doses two to four times a day, or in sustained release form. For most large mammals the total daily dosage is from about 0.07 g to 7.0 g, preferably from about 100 mg to 1000 mg. Dosage forms suitable for internal use comprise from about 100 mg to 500 mg of the active compound in intimate admixture with a solid or liquid pharmaceutically acceptable carrier. This dosage regimen may be adjusted to provide the optimal therapeutic response. For example, several divided doses may be administered daily or the dose may be proportionally reduced as indicated by the exigencies of the therapeutic situation. The production of the above-mentioned pharmaceutical compositions and medicaments is carried out by any method known in the art, for example, by mixing the active ingredients(s) with the diluent(s) to form a pharmaceutical composition (e.g. a granulate) and then forming the composition into the medicament (e.g. tablets). The following examples describe in detail the chemical synthesis of representative compounds of the present invention. The procedures are illustrations, and the invention should not be construed as being limited by chemical reactions and conditions they express. No attempt has been made to optimize the yields obtained in these reactions, and it would be obvious to one skilled in the art that variations in reaction times, temperatures, solvents, and/or reagents could increase the yields. Isoindoline was synthesized employing the method of R. E. Gawley, S. R. Chemburkar, A. L. Smith, T. V. Anklekar J. Org. Chem. 1988, 53, 5381. To 3,4-difluoronitrobenzene (3.02 mL, 27.3 mmols) in ethyl acetate at rt was added diisopropylethylamine (5.03 mL, 28.9 mmols) and then isoindoline (3.50 g, 29.4 mmols) and stirred overnight. A yellow precipitate (ppt) formed and was collected on a filter, washed with water and ether and dried in a vacuum oven (30°C) to provide the product as a bright yellow solid (6.69 g, 95% yield). Mp = 200-202°C. MS (M + 1) = 327 m/z. To the above nitro compound (2.62 g, 10.2 mmols) in ethanol (100 mL) was added SnCI2 (9.84 g, 50.9mmols) and was refluxed for 16hrs. After cooling to rt the reaction mixture was added to 10% aq. NaOH (300 mL) and extracted with CH2CI2 (6x50 mL). The combined organic washings were washed with brine (100 mL), dried over Na2SO4 and concentrated to give 2.63 g of an olive green solid (aniline), which was used without further purification. To this aniline in acetone (150 mL) and water (20 mL) was added NaHCO3 (1.84 g, 21.9 mmols) and then benzylchloroformate (1.68 mL, 11.8 mmols). After stirring overnight the mixture was poured into ice water (100 mL) and the resulting tan precipitate was collected on a filter, washed with water and dried in a vacuum to give the Cbz aniline as a tan solid (3.50 g, 95% yield). Mp = 146-148°C. MS (M + 1) = 363 m/z. Step 3: To the above Cbz aniline (0.74 g, 2.04 mmols) in THF (10 mL) at -78ºC was added n-BuLi (2.5 M, 0.82 mL, 2.05 mmols) dropwise. After stirring for 40 min, (R)-glycidyl butyrate (0.31 mL, 2.10 mmols) in THF (0.5 mL) was added dropwise and the resulting mixture was allowed to warm to RT overnight. A white precipitate had formed and was collected on a filter and washed with water and ether. Chromatography on silica gel with 25% ethyl acetate/hexane as eluent provided the product as a white solid (0.58 g, 87% yield). MS (M + 1) = 329 m/z. To the oxazolidinone carbinol from Example 1 (0.58 g, 1.78 mmols), in DMF (10 mL) and acetonitrile (10 mL) at 0°C was added triethylamine (0.74 mL, 5.31 mmols) and, after 10 min, methanesulfonyl chloride (0.2:8 mL, 3.62 mmols). After allowing the reaction mixture to warm to RT over an hour starting material was still present so cooling and addition of tniethyl amine (0.37 mL, 2.65 mmols) and methanesulfonyl chloride (0.14 mL, 1.81 mmols) was repeated. The mixture was poured into water (50 mL) and extracted with CH2CI2 (6 X 20 mL), washed with brine (4 x 10mL), dried over Na2SO4, concentrated to afford the crude product as a brown oil (0.95 g). MS (M + 1) = 407 m/z. To the mesylate from Example 2 (0.95 g, 1.78 mmols) in DMF (25 mL) was added sodium azide (0.47 g, 7.23 mmols) and heated to 70ºC for 16 hrs. After cooling to rt water was added and the mixture extracted with ethyl acetate (6X25 mL), washed with brine (4x10 mL), dried over Na2SO4, concentrated to give 0.48 g of a tan solid. MS (M + 1) = 354 m/z. Compound 1 The azide from Example 3 in ethyl acetate (25 mL) was placed in a Paar flask and nitrogen bubbled through for 15 min whereupon 10% Pd/C (0.15 g, 0.14 mmol) was added. The mixture was pressurized with 50 psi of H2 (g) and shaken for 16 hrs whereupon an additional amount of 10% Pd/C (0.15 g, 1.4 mmols) was added and the mixture shaken for an additional 6 hrs (at this point MS (M + 1) = 328 mlz). After placing the mixture under nitrogen, pyridine (0.22 mL, 2.72 mmol) and then Ac2O (0.51 mL, 5.30 mmol) were added and the mixture strred for 2 hrs. The mixture was filtered through celite, washing with ethyl acetate (100 mL), concentrated, and chromatographed on silica (gradient elution 1%-5% MeOH/CH2Cl2) and then triturated with ethyl acetate (3X3 mL) to give 0.19 g of a white solid (Compound 1, 29% yield for 4 steps). Mp = 240-242°C. MS (M + 1) = 370 m/z. Example 5 6.7-Dihydro-6-(2-fluoro-4-nitrophenvl)-5H-pyrrolo[3.4-b]pyridine: To 6,7- dihydro-5H-pyrrolo[3,4-b]pyridine dihydrochloride salt (as described by Petersen, et al. (Bayer) EP0520277A2)(42.8 g, 222 mmols) in DMF (1.2 L) was added 2,4-difluoronitrobenzene (25 mL, 224 mmols). The mixture was heated to 60°C and DIPEA (195 mL, 1.12 mols) was added dropwise from an addition funnel over 2 hrs. After heating overnight the reaction mixture was cooled to rt, poured into water (3 L), filtered and dried in a vacuum oven (50ºC) to provide a yellow-green solid (53.8 g, 94% yield). MS (M + 1) = 260 m/z. Step 2: 6,7-Dihydro-6-(2-fluoro-4-aminophenyl)-5H-pyrrolo[3,4-b]pyricline To the above nitro compound (53.8 g, 208 mmol) in THF (175 mL) and methanol (600 mL) was added ammonium formate (59.0 g, 907 mmol). Nitrogen was bubbled through the reaction for approximately 30 minutes whereupon 10% Pd/C (2.20 g, 21 mmols) was added. After stirring overnight at rt under an atmosphere of nitrogen the reaction mixture was filtered through a pad of Celite, washing thoroughly with methanol (400 mL), and concentrated to a volume of ca. 200 mL. Water (300 mL) was added and the mixture extracted with ethyl acetate (5X200 mL). The combined organic layers were washed with brine, dried (Na2SO4), filtered, and utilized directly in the next step without further purification. MS (M + 1) = 230 m/z. 6,7-Dihydor-6-(2-fluoro-4-(Aminocarboxybenzyl)phenyl)-5H-pyrrolo[3,4- b]pyridine The above aniline (~208 mmols) in acetone (1 L) and water (160 mL) was cooled to 0°C whereupon sodium bicarbonate (37.4 g, 445 mmols) was added followed by the dropwise addition of benzylchloroformate (34.2 mL, 228 mmols). The reaction mixture was allowed to warm to room temperature and stirred overnight whereupon a ppt formed. The reaction was poured into ice water (2 L) and the resulting precipitate was collected by o filtration. The solid was washed with water and dried in a vacuum oven (50 C) to afford the Cbz derivative (73.0 g, 97% yield) as a salmon colored powder. MS (M + 1) = 364 m/z. Step 4: (Compound 2). The above Cbz derivative (40.8 g, 112 mmols) in THF (1 L) was cooled to -78ºC under a nitrogen atmosphere. To this mixture was added n-BuLi (2.5 M, 45.8 mL, 114.5 mmols) dropwise via syringe over fifteen minutes. The reaction was warmed to room temperature and allowed to stir for 45 minutes before again being cooled to -78ºC. At this point (R)-glycidyl butyrate (17.2 mL, 117 mmols) was added and the reaction mixture allowed to warm to rt overnight during which time a precipitate formed. The ppt was collected, washed with several portions of ether (5X100 mL) and dried in a vacuum oven (50ºC) to afford 40.6 g of the ether solvate of the lithium alkoxide as a tan fluffy powder. This material was then washed with several portions of water (4X200 mL) and dried in a vacuum oven (50ºC) to afford the oxazolidinone alcohol (34.1 g, 92% yield) as a tan granular solid. Mp = 208- 212°C, decomp. MS (M + 1) = 330 m/z. Example 6 Oxazolidinone Mesylate. The above oxazolidinone carbinol (from Example 4) (33.8 g, 103 mmols) was suspended in DMF (1.25 L, previously degassed with nitrogen) at rt under a nitrogen atmosphere. Triethylamine (50 mL, 360 mmols) was added followed by the dropwise addition of methanesulfonyl chloride (13.5 mL, 174 mmols). After stirring for 3 hrs the reaction mixture was poured into water (200 mL) and methylene chloride (1 L) added. A ppt was filtered off, washed with water (3X200 mL) and dried in a vac oven (50ºC) to afford the mesylate as a tan solid (28.1 g, 67%). The organic layer was dried (Na2SO4), filtered and evaporated to also afford the mesylate (11.7 g, 28% yield) as a tan solid. Both where characterized with MS (M + 1) = 408 m/z. Oxazolidinone Azide. The above mesylate (from Example 5) (27.8 g, 68.2 mmols) and sodium azide (17.7 g, 271 mmols) in anhydrous DMF (1 L), previously degassed with nitrogen, were heated 95ºC for 6 hr under a nitrogen atmosphere. After cooling, the mixture was poured into stirred ice water (2 L) and formed a flocculant white ppt. The ppt was collected on a filter and washed with water (4X200 mL), dried in a vac oven (50ºC) to afford the azide as a light beige solid (22.7 g, 94% yield). Mp = 175-180 C, decomp. MS (M + 1) = 355m/z. Example 8 Oxazolidinone Acetamide. The above azide (from Example 6)(21.67 g, 61.16 mmol) dissolved in DMF (400 mL) and THF (500 mL) was degassed with nitrogen for 30 minutes whereupon 10% Pd/C (4.74 g, 4.4 mmols) was added and the reaction hydrogenated on a Parr apparatus (60 psi of hydrogen) for 14 hr. The reaction mixture was removed from the Parr apparatus and placed under a nitrogen atmosphere whereupon pyridine (5.44 mL, 67.3 mmols) and acetic anhydride (6.35 mL, 67.3 mmols) were added. After stirring for 1 hr the reaction mixture was filtered through a pad of Celite, washing thoroughly with methanol and then copious amounts of 50% MeOH/CH2CI2 (ca. 2 L). The filtrate was evaporated to afford the crude acetamide in DMF. The mixture was slowly added to water (2 L) and the ppt collected on a filter, washed with water (5X400 mL) and dried in a vac oven (50ºC) to provide the acetamide as an analytically pure white solid (14.2 g, 63% yield). The combined filtrates were extracted with methylene chloride (5X200 mL), dried over Na2SO4 and concentrated. Water was added to the residue and the resulting ppt was filtered off and dried in a vac oven (50ºC) to afford a second crop of the acetamide as a light tan, fluffy solid (5.61 g, 25%). For the analytically pure material Mp = 229-230ºC, decomp. MS (M + 1) = 371 m/z. Example 9 The above acetamide from Example 8 (2.51 g, 6.78 mmols) was taken up in CH2CI2 and MnO2 added (23.9 g, 234 mmols). After stirring overnight the reaction mixture was filtered through celite, concentrated and chromatography on silica with 10% MeOH/CH2Cl2 as eluent to afford the product as a light yellow solid (0.48 g, 19% yield). Mp = 220-225°C decomp. MS (M + 1) = 369 m/z. Example 10 Compound 5 was prepared as in Example 8 except (S)-glycidyl butyrate was employed in the oxazolidinone formation. The product was isolated as a light tan solid. Mp = 227-230°C decomp. MS (M + 1) = 371 m/z. Example 11 Compound 6 was prepared as in Example 9 and isolated as a light yellow solid. Mp = 181-185 C decomp. MS (M + 1) = 369 m/z. To 5-hydroxyisoxazole (prepared as in Chem Pharm Bull 1966, 14(11), 1277) (0.174 g, 2.04 mmols) in DMF was added NaH (60% in oil)(0.105 g, 2.62 mmols). After stirring for 30 min the mesylate (from Example 6) (0.744g, 1.82 mmols) was added in one portion and the mixture stirred at 60ºC overnight. After cooling to rt water was added and a ppt was collected on a filter, air dried and chromatographed on silica with 2.5% MeOH/CH2Cl2 as eluent to afford the product as a white solid (0.140 g, 19 % yield). Mp = 182-185ºC. MS (M + 1) = 397 m/z. Example 13 To the above oxazolidinone (from Example 12) (0.264 g, 6.66 mmols) was taken up in CH2CI2 and MnO2 added (1.66 g, 16.2 mmois) in two portions over two days. After stirring for two days the reaction mixture was filtered through celite, concentrated and chromatographed on silica with 10% MeOH/CH2Cl2 as eiuent to afford the product as a light yellow solid (0.086 g, 32% yield). Mp = 133-135°C. MS (M + 1) = 395 m/z. Compound 9 To NaH (60% by wt in oil)(0.03 g, 0.76 mmol) in DMF (5 mL) was added oxazolidinone carbinol (from Example 5) (0.23 g, 0.71 mmol) in four portions. After stirring for 30 min 2-chloropyrazine (0.065 mL, 0.71 mmol) was added via syringe and stirred overnight at rt. Water was added and a ppt was collected on a filter, air dried and chromatographed on silica with 5% MeOH/CH2CI2 as eluent to afford the product as a white solid (0.067 g, 23 % yield). Mp = 225-230'C. MS (M + 1) = 408 m/z. The above oxazolidinone (from Example 14) (0.024 g, 0.058 mmol) in CH2CI2 (5 mL) was added MnO2 (0.07 g, 0.7 mmol). After stirring overnight the reaction mixture was filtered through Celite and concentrated to afford the product as a very light yellow solid (0.015 g, 64% yield). Mp = 192-194 C. MS (M + 1) = 406 m/z. Compound 11 To a suspension of the oxazolidinone carbinol (prepared in Example 5) (330 mg, 1.0 mmol), triphenylphosphine (260 mg, 1.1 mmols) and 4-hydroxy-1, 2, 5-thiadiazole (100 mg, 1.0 mmol) (as prepared in U.S Patent 3,391,150 [7/2/68]) in THF (8 mL) was added diisopropylazodicarboxylate (0.20 mL, 1.1 mmols). After stirring overnight at rt the reaction mixture was filtered, washed with methanol, and air dried to afford a yellow crystalline solid (60 mg, 15% yield). Mp = 185-187°C. MS (M + 1) = 414 m/z. To the oxazolidinone (prepared in Example 16) (160 mg, 0.39 mmoi) suspended in CH2CI2 (1.0 mL) was added MnO2 (four additions of 150 mg over four days). The reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (15 mL), and concentrated under reduced pressure to afford the product as a white crystalline solid (63 mg, 40% yield). Mp = 185- 188°C. MS(M + 1) = 412 m/z. To the amine (as prepared in Example 8) (100 mg, 0.30 mmol) and potassium carbonate (100 mg, 0.72 mmol) suspended in methanol (1.0 mL), was added propionyl chloride (50 mg, 0.54 mmol). After stirring overnight at 80 C the reaction mixture was cooled and water was added. A precipitate was filtered off, washed with methanol and air dried to afford the product as a brown crystalline solid (15 mg, 13 % yield). Mp = 110-112ºC. MS (M + 1) = 385 m/z. To the amide (prepared in Example 18) (15 mg, 0.04 mmol) suspended in CH2CI2 (1.0 mL), was added MnO2 (200 mg) at rt. After stirring overnight, the reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10 mL), and concentrated under reduced pressure to afford the product as an light brown crystalline solid (1.6 mg, 8 % yield). MS (M + 1) = 383 m/z. To the amine (as prepared in Example 8) (60 mg, 0.18 mmol) and potassium acetate (60 mg, 0.61 mmol) suspended in methanol (1.0 mL), was added cyclopropyl carbonyl chloride (120 mg, 1.15 mmols). After stirring at rt overnight, the reaction mixture was filtered, rinsed with methanol, and then concentrated to dryness under reduced pressure. The resulting solid residue was triturated with water and filtered to afford the product as a brown crystalline solid (36 mg, 50 % yield). Mp = 235-240°C. MS (M + 1) = 397 m/z. Example 21 To the amide (prepared in Example 20) (36 mg, 0.09 mmol) suspended in CH2CI2 (1.0 mL), was added MnO2 (three portions of 100 mg over three days) at rt. The reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10 mL), and concentrated under reduced pressure to afford the product as an off-white crystalline solid (3 mg, 8 % yield). MS (M + 1) = 395 m/z. Example 22 To the amine (prepared in Example 8) (60 mg, 0.18 mmol) and potassium acetate (60 mg, 0.61 mmol) suspended in methanol (1.0 mL), was added dropwise methyl chloroformate (120 mg, 1.27 mmols). After stirring for four hours at rt, the reaction mixture was filtered, diluted with water, and concentrated under reduced pressure to remove the methanol. The aqueous solution was extracted with ethyl acetate (5X5 mmL). The combined organics were washed with water, dried over MgSO4, filtered, and concentrated to provide an oil which was triturated with ether to afford a brown crystalline solid (35 mg, 50% yield). MS (M + 1) = 387 m/z. Example 23 To the carbamate (prepared in Example 22) (33 mg, 0.08 mmol) suspended in CH2CI2 (1-0 mL), was added MnO2 (150 mg). After stirring overnight at rt the reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10 mL), and concentrated under reduced pressure to afford the product as a yellow crystalline solid (6.0 mg, 18% yield). MS (M + 1) = 385 m/z. Example 24 Compound 19 To the amine (prepared in Example 8) (60 mg, 0.18 mmol) and potassium acetate (60 mg, 0.61 mmol) suspended in methanol (1.0 mL) was added dropwise ethyl chloroformate (0.1 mL, 1.04 mmols). After stirring overnight at rt the reaction mixture was filtered, diluted with water, and concentrated under reduced pressure to remove the methanol. The aqueous solution was extracted with ethyl acetate (5X5 mL). The combined organics were washed with water, dried over MgSO4, filtered, and concentrated. The resulting semi- solid was treated with water, filtered and air-dried to afford a brown crystalline soiid (18 mg, 30% yield). MS (M + 1) = 401 m/z. To the amine (prepared in Example 8) (95 mg, 0.29 mmol) suspended in pyridine (0.5 mL) was added methane sulfonylchloride (0.08 mL, 1.0 mmol). After stirring overnight at rt the pyridine was removed under a stream of nitrogen. The residue was treated with water, filtered and air-dried to afford a brown solid (45 mg, 38% yield). Mp = 172-176°C. MS (M + 1) = 407 m/z. To the sulfonamide (prepared in Example 25) (10 mg, 0.02 mmol) suspended in CH2CI2 (1.0 mL), was added MnO2 (100 mg, 10 mmols). After stirring overnight the reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10 mL), and concentrated under reduced pressure to afford the product as a brown crystalline solid (0.5 mg, 5% yield). MS (M + 1) = 405 m/z. Compound 22 To the amine (prepared in Example 8) (200 mg, 0.61 mmol) suspended in toluene (8 mL), was added dimethyl-N-cyanodithioiminocarbonate (89 mg, 0.61 mmol). After stirring overnight at reflux the toluene was decanted and the oily residue treated with methanol, filtered, and air-dried to afford a brown crystalline solid (62 mg, 20% yield). Mp = 204-207°C. MS (M + 1) = 427 m/z. Compound 23 A suspension of the thioimidate (from Example 27) (45 mg, 0.10 mmol) and MnO2 (200 mg, 2.0 mmols) in CH2CI2 were stirred at rt for one day whereupon a second addition of MnO2 (150 mg, 1.5 mmols) was added. After an additional day of stirring the mixture was filtered through Celite, washed with CH2CI2 (10 mL), concentrated to afford a yellow crystalline solid (20 mg, 45% yield). MS (M + 1) = 426 m/z. A suspension of the amine (prepared in Example 8) (165 mg, 0.5 mmol) and 2-methyl-1-nitro-2-thiopseudourea (94 mg, 0.70 mmol) (as prepared as in EP 0539204/1993) in methanol (2 mL) was refluxed for four hours. After cooling to rt the reaction mixture was filtered and air dried to afford a yellow crystalline solid (50 mg, 24% yield). Mp = 202-206°C. MS (M + 1) = 416 m/z. To the nitroguanidine (prepared in Example 29) (35 mg, 0.08 mmol) suspended in CH2CI2 (1.0 mL) was added MnO2 (three additions of 100 mg over three days). The reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10 mL), and concentrated under reduced pressure to afford the product as a yellow crystalline solid (1.6 mg, 4% yield). IMS (M + 1) = 414 m/z. The starting material 6,7-dihydro-5H-pyrrolo[3,4-c]pyridine was prepared as in US Pat. No. 5,371,090 to Petersen et al. Compound 26 was then prepared as in Example 8 except the acetamide was recrystallized from acetonitrile to give a light tan solid. Mp = 182-190ºC decomposition. MS (M + 1) = 371 m/z. Compound 27 was isolated from the final step of Example 31 via chromatography (5% MeOH/CH2CI2 as eluent) of the mother liquors collected from recrystallization. Light yellow solid, Mp = 219-225ºC decomp. MS (M + 1) = 385 m/z. Compound 28 was prepared as in Example 9 except with 10% MeOH/CH2Cl2 as eluent. Light yellow solid, Mp = 219-225ºC decomposition. MS (M + 1) = 369 m/z. Isothiazole (0.088 g, 0.87mmol)(prepared as in J Heterocyclic Chem 1971, 8, 591) was added portionwise at rt to a suspension of sodium hydride (0.036 g, 0.91 mmol, 60% in oil) in DMF (4 mL) under nitrogen. The mixture was stirred for 30 minutes whereupon the mesylate from Example 31 (0.31 g, 0.76 mmol), in DMF (10 mL), was added all at once. After stirring for 6 hours at 60ºC the reaction mixture was cooled to rt, diluted with water (50 mL), and extracted with ethyl acetate (3x50 mL). The combined organics were washed several times with water, then once with brine, dried over sodium sulfate, concentrated, and chromatographed on silica with 5% MeOH/EtOAc as eluent. Two products were isolated from the chromatography: 0.050g of Compound 29; and 0.022 g of Compound 30. Overall yield, 30%. Compound 29 MS (M+1) = 413.0 Compound 30 MS (M+1) = 411.1 Example 35 To a suspension of sodium hydride (0.036 g, 0.91 mmol, 60% in oil) in DMF (4 mL) at rt under nitrogen was added portion wise 4-hydroxy-1, 2, 5-thiadiazole (0.088 g, 0.87 mmol) (as prepared in U.S Patent 3,391,150 [7/2/68]). After stirring for 30 min the mesylate from Example 31 (0.310 g, 0.76 mmol), in DMF (10 mL), was added all at once. After stirring for 6 hours at 60 C the reaction mixture was cooled to rt, diluted with water (50 mL), and extracted with ethyl acetate (3x50 mL). The combined organics were washed several times with water, then once with brine, dried over sodium sulfate, concentrated, and chromatographed on silica with 2% MeOH/EtOAc as eluent. Two products were isolated from the chromatography: 0.035 g of Compound 31; and 0.0093 g of Compound 32. Overall yield, 14%. Compound 31 MS (M+1 ) = 414.0 Compound 32 MS (M+1 ) = 412.1 To the mesylate from Example 31 (2.45 g, 6.01 mmol) dissolved in degassed DMF (100 mL) under nitrogen was added potassium phthalimide (2.23 g, 12.0 mmols). After heating at 65ºC for 3 hours the reaction mixture was cooled, poured into water (300 mL), and extracted with methylene chloride (3x200 mL). The combined organics were washed with water (3x150 mL) dried over sodium sulfate, concentrated to a tan solid. This solid was washed with water and dried in a high vacuum oven at 50ºC to afford 2.20 g (80%) of the oxazolidinone phthalimide. MS= 459.1 (M+1) Step 2: To the above phthalimide (0.97 g, 2.1 mmols) in degassed methanol (30 mL) under nitrogen was added hydrazine monohydrate (0.2 mL, 4.3 mmols) dropwise. After refluxing for 12 hours the reaction mixture was cooled to rt, and concentrated, suspended CH2CI2 and filtered. The crude oxazolidinone amine was concentrated and used without further purification. Step 3: Compound 33, To the crude amine (0.14 g, 0.44 mmol) in CH2CI2 (5 mL) was added pyridine (0.14 mL, 18 mmols) followed by propionyl chloride (0.76 mL, 0.88 mmol). After stirring for 5 hrs at rt the solution was poured into water (20 mL) and extracted with methylene chloride (3x10 mL). The combined extracts were washed with water (10 mL) and 1 M NaOH (aq) (10 mL), dried over sodium sulfate, concentrated and chromatographed using neat EtOAc as eluent to afford the propionyl amide as a gold oil (0.020 g, 12% yield). MS= 385.2 (M+1) To the crude amine (as prepared in Example 36) (0.144 g, 0.437 mmol) in methylene chloride (5 mL) was added pyridine (0.14 mL, 1.7 mmols), followed by cyclopropane carbonyl chloride (0.08 mL, 0.88 mmol). After stirring for 5 hrs at rt the solution was poured into water (20 mL) and extracted with methylene chloride (3x10 mL). The combined extracts were washed with water (10 mL) and 1 M NaOH (aq) (10 mL), dried over sodium sulfate, concentrated and chromatographed using a gradient elution of 1% to 5% to 10% MeOH/ EtOAc. The desired product eluted with 5% MeOH/ EtOAc and was concentration to afford the product as a white powder (0.012 g, 7% yield). MS= 397.2 (M+1) To N-[(3-pyrrolidinone-3-fluorophenyl) 5-oxazolidinyl]methyl acetamide (prepared according to W096/13502)(0.150 g, 0.447 mmols) was added methoxy-bis(dimethylamino)methane (1 mL). After heating at 50 C for 15 min the reaction mixture was concentrated to provide the crude ß-ketoenamine which was used without further purification. Step 2; Compound 35 To ethanolic NaOEt (made from 0.027 g Na in 3 mL EtOH) was added acetamidine hydrochloride (0.113 g, 1.19 mmols) and the above ß- ketoenamine oxazolidinone acetamide. After refluxing for 3 hrs the reaction mixture was cooled to rt, concentrated, taken up in chloroform, and washed with water (3x8 mL). After drying over sodium sulfate the crude product was concentrated, dissolved in 5% MeOH/ EtOAc, and filtered to afford the product as an off-white solid (0.052 g, 45% yield). Mp = 234°C, decomp. MS = 385.9 (M+1) Example 39 To N-[(3-pyrrolidinone-3-fluorophenyl) 5-oxazolidinyi]methyl acetamide (prepared according to W096/13502)(0.099 g, 0.29 mmol) was added methoxy-bis(dimethylamino)methane (1.0 mL). After heating at 50ºC for 2 hrs the reaction mixture was concentrated to provide the crude ß-ketoenamine. To this mixture was added benzene (5 mL), DMF (1 mL) and formamidine acetate (0.55 g, 5.3 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2 as eluent to afford the product as a white powder (0.037 g, 34% yield). Mp = 230-232°C. MS (M + 1) = 372 rn/z. The above acetamide from Example 39 (0.020 mg, 0.054 mmol) was taken up in CH2CI2 (5 mL) and MnO2 added (0.10 g, 0.98 mmol). After stirring overnight at rt the reaction mixture was filtered through Celite and concentrated to afford the product as a light yellow solid (0.016 g, 80% yield). Mp = 164-166°C. MS (M + 1) = 370 m/z. Example 41 To the ß-ketoenamine (prepared as in Example 39) was added benzene (5 mL), DMF (1 mL) and pyrazine-2-carboxamidine hydrochloride (0.62 g, 3.9 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2 as eluent to afford the product as a light yellow solid (0.0026 g, 2% yield). Mp = 212-214°C. MS (M + 1) = 450 m/z. The above acetamide from Example 39 (0.040 g, 0.088 mmols) was taken up in CH2CI2 (10 mL) and MnO2 (0.36 g, 3.5 mmols) added in three portions over three days. After stirring for three days the reaction mixture was filtered through Celite, concentrated and chromatography on silica with 7% MeOH/CH2Cl2 as eluent. Two products were isolated from the chromatography: 0.001 g of Compound 39 as a light yellow solid (4% yield); and 0.002 g of Compound 40 as a yellow solid (4% yield). Compound 39: MS (M + 1) = 448 m/z. Compound 40: MS (M + 1) = 464 m/z. Example 43 Compound 41 To the ß-ketoenamine (prepared as in Example 39) was added benzene (5 mL), DMF (1 mL) and 4-amidinopyridine hydrochloride (0.81 g, 5.2 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2 as eluent to afford the product as a light yellow solid (0.072 g, 55% yield). Mp = 245-250°C, decomp. MS (M + 1) = 449 m/z. Example 44 Compound 42 To the ß-ketoenamine (prepared as in Example 39) was added benzene (5 mL), DMF (1 mL) and 2-amidinopyridine hydrochloride (0.61 g, 3.9 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2 as eluent to afford the product as a yellow powder (0.054 g, 40% yield). Mp = 216-220°C. MS (M + 1) = 449 m/z. To the ß-ketoenamine (prepared as in Example 39) was added benzene (5 mL), DMF (2 mL) and 3-amidinopyridine hydrochloride (0.49 g, 3.1 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2Cl2 as eluent to afford the product as a light purple, crystalline solid (0.044 g, 33% yield). Mp = 265-270°C, decomp. MS (M + 1) = 449 m/z. To the ß-ketoenamine (prepared as in Example 39) was added benzene (5 mL), DMF (2 mL) and hydrazine hydrochloride (0.22 g, 3.2 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2 as eluent to afford the product as off-white powder (0.022 g, 21% yield). Mp = 244-247°C, decomp. MS (M + 1) = 360 m/z. To the ß-ketoenamine (prepared as in Example 39) was added benzene (5 mL), DMF (2 mL) and n-propylhydrazine oxalate (0.87 g, 5.3 mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt and water (8 mL) was added. A ppt formed and was collected by filtration, dried in a o vacuum oven (50 C), and chromatographed on silica with 5% MeOH/CH2Cl2 as eluent to afford the product as a light yellow solid (0.081 g, 55% yield). Mp = 204-208°C. MS (M + 1) = 402 m/z. The starting material aniline (N-[[(5S)-3-(4-amino-3-fluorophenyl)-2-oxo-5- oxazolidinyl]methyl]-acetamide) was prepared as in World Patent WO 96/23788. To phthalic dicarboxaldehyde (0.0522 g, 0.378 mmol) in acetonitrile (1 mL) was added glacial acetic acid (0.05 mL, 0.87 mmol) and then the above aniline (0.0955 g, 0.357 mmol) in acetonitrile (5 mL) dropwise. After 4 hrs water (10 mL) was added and a precipitate was collected on a filter and washed with water and ether to provide Compound 46 as a light green solid (0.0655g, 48%). Mp = 211-214°C. MS (M + 1) = 384 m/z. Example 49 To starting material aniline (N-[[(5S)-3-(4-amino-3-fluorophenyl)-2-oxo-5- oxazolidinyl]methyl]-acetamide)(0.095 g, 0.36 mmol)(as prepared in World Patent WO 96/23788) in CH2CI2 (5 mL) was added triethylamine (0.15 mL, 1.1 mmols) and phthaloyl dichloride (0.056 mL, 0.39 mmol). After stirring overnight a solid was collected on a filter, washed with water (10 mL) and dried in vacuum oven (50 C) to afford the product as a off-white solid (0.060, 42%). Mp = 240-242°C. MS (M + 1) = 398 m/z. To starting material aniline (N-[[(5S)-3-(4-amino-3-fluorophenyl)-2-oxo-5- oxazolidinyl]methyl]-acetamide)(0.20 g, 0.75 mmol)(as prepared in World Patent WO 96/23788) in acetonitrile (5 mL) was added 2,3-pyndine dicarboxaldehyde (0.10 g, 6.6 mmols) and glacial acetic acid (0.050 mL, 0.87 mmol). After stirring for 5hrs the reaction mixture was concentrated and chromatographed on silica with 2.5% MeOH/CH2Cl2 as eluent to afford the two products: 0.035 g of Compound 52 (12%) as a yellow solid; and 0.011 g of Compound 53 (4%) as a yellow solid. Compound 48: Mp = 230-232°C. MS (M + 1) = 385 m/z. Compound 49: Mp = 207-209°C. MS (M + 1) = 385 m/z. The invention has been described in detail with particular reference to the above embodiments thereof. The above embodiments and examples are given to illustrate the scope and spirit of the present invention. These embodiments and examples will make apparent, to those skilled in the art, other embodiments and examples. These other embodiments and examples are within the contemplation of the present invention. It will be understood that variations and modifications can be effected within the spirit and scope of the invention; therefore, the instant invention should be limited only by the appended claims. We claim: 1. A compound of Formula I wherein: R is selected from the group consisting of OH, O-Aryl, O- Heteroaryl, N3, OR, OSO2R", -NR"'R"", or wherein: (i) R1 is straight-chain or branched acy! having up to 6 carbon atoms or benzyl; (ii) R"is straight-chain or branched alkyl, having up to 5 carbon atoms, phenyl or tolyl; and (iii) R"' and R"" are independently selected from the group consisting of H, cycloalkyl having 3 to 6 carbon atoms, phenyl or tert- butoxycarbonyl, fluorenyloxycarbonyl, benzyloxycarbonyl, straight- chain or branched alkyl having up to 6 carbon atoms which is optionally substituted by cyano or alkoxycarbonyl having up to 4 carbon atoms, -CO2-R1, -CO-R1, -CS-R1, and -SO2-R4, In which R1 is selected from the group consisting of H, cycloalkyl having 3 to 6 carbon atoms, trlfluoromethyl or phenyl, benzyl or acyl having up to 5 carbon atoms, straight-chain or branched alky! having up to 6 carbon atoms, said alkyl optionally substituted by straight-chain or branched alkoxycarbonyl having up to 5 carbon atoms, OH, cyano, up to 3 halogen atoms, and -NR6 R6 in which R5 and R6 are identical or different and are selected from H, phenyl or straight-chain or branched aikyl having up to 4 carbon atoms; R4 is selected from straight-chain or branched aikyi having up to 4 carbon atoms or phenyl and; R4a is CN, COR4c, COOR4a, CONHR4c, CO-NR4c, R4d, SO2R4c, or NO2; R4b is H, alkyl, OR4c SR4c amino, NHR4c NR4c R4c; R4c and R4d are independently selected from H, alkyl, aryl, or in the case of any NR4c R4d, group R4c and R4d taken together with the nitrogen atom to which they are attached form a unsubstituted or substituted pyrrolidine piperidinyl or morphoiinyi group; X is 0 to 4 members independently selected from the group consisting of halogen, OH, nitro, C1-8 alkoxy, C1-8 alkyl-amino, di(C1-8- alkyl-) amino, carboxy, alkoxycarbonyl, C1-8 alkyl-CO-O-, C1-8 alkyl- CO-NH-, carboxamide , CN, amine, C3-6 cycloalkyl, C1-8 alkyl optionally substituted with one or more members selected from the group consisting of F, CI, OH; and Y is a radical of Formulae II or III: wherein R5, R6, R7 and R8 are each Independently H, alkyl, CN, nltro, C1-8- alkyl, formyl, carboxy, alkoxy-carbonyl, carboxamide, or R6 and R6 and/or R7 and R6 together form an oxo group; R9, and R10 are each independently H, halogen, alkyl, OH, CN, nitro, C1-8 alkyl, halo-C1-8-alkyl, C1-8 alkoxyl, amino, C1-8-alkyl-amino, di (C1- 8-alkyl-) amino, formyl, carboxyl, alkoxycarbonyl, C1-8-alkyl-CO-O-, C1- 8-alkyl-CO-NH-, carboxamide, or amine; is a fused phenyl ring or a five-or six-membered heteroaromatlc ring having one to four members selected from the group consisting of S, 0, and N; Z is selected from halogen, alkyl, aryl, substltuted-aryl, heteroaryl, substltuted-heteroaryl CN. CHO, Coalkyl, amine, (dlallkyiamino) alkyl where dialkyiamlno is selected from dimethylamine, diethylamine, morpholinyl, thiomorphollnyl, pyrroldlnyl, or plperidinyl, or, alkoxy, or NHCO-CC1-C8-alkyl); and m is 0 to 1, and the pharmaceutical acceptable salts and esters thereof. 2. The compound as claimed in claim 1 wherein Y is selected from the group consisting of 3. The compound as claimed in claim 1 wherein R is -NHCOCH3 or is selectedfrom the group consisting of 4. A compound as claimed in claim 1 having the formula: 5. A pharmaceutical composition comprising a compoundas claimed in any of claims 1 to 4 and a pharmaceutically acceptable carrier. 6. A compound as claimed in any one of claims 1 to 4 or a composition as claimed in claim 5, for use in a method of treating a subject having, or preventing a subject from suffering from a condition caused by or contributed to by bacterial infection, such as community-acquired pneumonia, upper or lower respiratory tract Infection, skin or soft tissue Infection, bone or joint Infection or a hospital acquired lung infection, such as caused by S. aureus, S. epidermidls, S. pneumoniae, S. pyogenes, Entenococcus spp., Moraxeiia catarrhalis H influenzae, a Gram-positive coccus or a Gram-positive coccus which is drug-resistant. Bicycllc heterocyclic substituted phenyl oxazoildinone compounds of formula (I): wherein Y is a radical of formulae (II) or (III) In which the substituents have the meaning indicated in the description. These compounds are useful as antibacterial agents.

Full Text

ANTIBACTERIAL HETEROBICYCLIC SUBSTITUTED PHENYL OXAZOLIDINONES
FIELD OF THE INVENTION
The present invention relates to the field of phenyl oxazolidinone
compounds having antibacterial activity against Gram-positive and Gram-
negative bacteria, pharmaceutical compositions containing the compounds,
and methods of treating bacterial infections with the compounds.
BACKGROUND OF THE INVENTION
Oxazolidinones have been identified, within the last twenty years, as a
new class of antibacterials which are active against numerous multidrug-
resistant gram positive organisms. Particularly problematic pathogens include
methicillin-resistant Staphylococcus aureus (MRSA), glycopeptide-
intermediate resistant Staphylococcus aureus (GISA), vancomycin-resistant
enterocci (VRE) and penicillin- and cephalosporin-resistant Streptococcus
pneumoniae. As a class, oxazolidinones exhibit a unique mechanism of
action. Studies have shown that these compounds selectively bind to the 50S
ribosomal subunit and inhibit bacterial translation at the initiation phase of
protein synthesis. Exemplary members of oxazolidinones are linezolid (see
WO 95/07271) and eperezolid.
U.S. Pat. No. 5,792,765 to Riedi et al. discloses a series of substituted
oxazolidinones (cyanoguanidine, cyanoamidines, and amidines) useful as
antibacterial medicaments.
U. S. Patent No. 5,910,504 to Hutchinson discloses a series of hetero-
aromatic ring substituted phenyl oxazolidinones, including indolyl substituted
compounds useful as antibacterial agents.
WO 98/54161 (Hester et al.) discloses amides, thioarnides, ureas, and
thioureas which are antibacterial agents.
WO 95/07271 (Barbachyn et al.) discloses oxazine and thiazine
oxazolidinone derivatives such as linezolid and its analogs which are useful
antimicrobial agents, effective against a number of human and veterinary
pathogens, including gram-positive aerobic bacteria such as multiple-resistant
staphylococci, streptococci and enterococci as well as anaerobic organisms
such as Bacteroides spp. and Clostridia spp. species, and acid-fast organisms
such as Mycobacterium tuberculosis, Mycobacterium avium and
Mycobacterium spp.
WO 93/09103 (Barbachyn et al.) discloses substituted aryl- and
heteroarylphenyloxazolidinones which are useful as antibacterial agents.
SUMMARY OF THE INVENTION
The invention provides phenyl oxazolidinone compounds of Formula I:
wherein:
R is selected from the group consisting of OH, N3, OR', O-Aryl, O-Heteroaryl
OSO2R", -NR"R", or

(i) R' is straight-chain or branched acyl having up to 6 carbon atoms or benzyl;
(ii) R" is straight-chain or branched alky!, having up to 5 carbon atoms, phenyl
or tolyl; and
(iii) R'" and R"" are independently selected from the group consisting of H,
cycloalkyl having 3 to 6 carbon atoms, phenyl or tert-butoxycarbonyl,
fluorenyioxycarbonyl, benzyloxycarbonyl, straight-chain or branched alkyl
having up to 6 carbon atoms which is optionally substituted by cyano or
alkoxycarbonyf having up to 4 carbon atoms, -CO2-R1, -CO-R1, -CO-SR1, -
CS-R1, P(O)(OR2)(OR3), and -SO2-R4, in which
R1 is selected from the group consisting of H, cycloalkyl having 3 to 6
carbon atoms, trifluoromethyl or phenyl, benzyl, or acyl each having up to
5 carbon atoms, straight-chain or branched alkyl having up to 6 carbon
atoms, said alkyl optionally substituted by straight-chain or branched
alkoxycarbonyl having up to 5 carbon atoms, OH, cyano, up to 3 halogen
atoms, and -NR5 R6 in which R5 and R6 are identical or different and are
selected from H, phenyl or straight-chain or branched alkyl having up to 4
carbon atoms;
R2 and R3 are identical or different and are selected from hydrogen or
straight-chain or branched alkyl having up to 4 carbon atoms; and
R4 is selected from straight-chain or branched alkyl having up to 4 carbon
atoms or phenyl and;
R4a is CN, COR4C, COOR4C, CONHR4C CO-NR4C R4d, SO2R4C. SO2NHR4c,
SO2-NR4C R4C, or NO2;
R4b is H, alkyl, OR4c, SR4c, amino, NHR4c, NR4c,R4c, C1-C8-alkylaryl or
mono-, di-, tri-, and per-halo C1-C8-alkyl;
R4c and R4d are independently selected from H, alkyl, aryl, or in the case of
any NR4CR4C group R4C and R4d taken together with the nitrogen atom to
which they are attached form a unsubstituted or substituted pyrrolidinyl,
piperidinyl or morpholinyl group;
X is 0 to 4 members independently selected from the group consisting of
halogen, OH, mercapto, nitro, halo-C1-8-alkyl, C1-8 alkoxy, thio-C-1-8-alkyl, C1-8
alkyl-amino, di(C1-8-alkyl-)amino, formyl, carboxy, alkoxycarbonyl, C1-8 alkyl-
CO-O-, C1-8 alkyl-CO-NH-, carboxamide, aryl, substituted-aryl, heteroaryl,
substituted-heteroaryl, CN, amine, C3-6 cycloalkyl, C1-8 alkyl optionally
substituted with one or more members selected from the group consisting of
F, CI, OH, C1-8 alkoxyl and C1-8 acyloxy; and
Y is a radical of Formulae II or III:
wherein
R5, R6, R7, and R8 are each independently H, alkyl, CN, nitro, C1-8 alkyl,
halo-C1-8-alkyl, formyl, carboxy, alkoxycarbonyl, carboxamide, aryl,
substituted-aryl, heteroaryl, substituted-heteroaryl, or R5 and R6 and/or R7
and R8 together form an oxo group;
R9, and R10 are each independently H, halogen, alkyl, OH, CN, mercapto,
nitro, C1-8 alkyl, halo-C1-8-alkyl, C1-8 alkoxy, thio-C1-8-alkyl, amino, C1-8-
alkyl-amino, di(C1-8-alkyl-)amino, formyl, carboxy, alkoxycarbonyl, C1-8-
alkyl-CO-O-, C1-8-alkyl-CO-NH-, carboxamide, aryl, substituted-aryl,
heteroaryl, substituted-heteroaryl, or amine;
Rg, and R10 are each independently H, halogen, alkyl, OH, CN, mercapto,
nitro, C1.8 alkyl, halo-d-e-aikyl, Ci.8 alkoxy, thio-d-s-alkyl, amino, d-8-
alkyl-amino, di(Ci.e-alkyl-)amino, formyl, carboxy, alkoxycarbonyl, C1-8-
alkyl-CO-O-, d-e-alkyl-CO-NH-, carboxamide, aryl, substituted-aryl,
heteroaryl, substituted-heteroaryl, or amine;
A, B, C, and D are selected from C, S, O, and N to form any five to ten
membered aromatic or heteroaromatic ring, said heteroaromatic ring
having one to four members selected from the group consisting of S, O,
and N;
Z is selected from halogen, alkyl, aryl, substituted-aryl, heiteroaryl,
substituted-heteroaryl, CN, CHO, COalkyl, amine, (dialkylamino)alkyl, said
dialkylamino consisting of straight-chain or branched alkyl having up to 6
carbon atoms or phenyl or constituting a ring of 2 to 5 carbons having 0 to
2 atoms selected from S, O and N or alkoxy, or NHCO-(C1-C8-alkyl); and
m is 0 or 1,
and the pharmaceutically acceptable salts and esters thereof.
Compounds of the above formula are useful as antibacterial agents for
the treatment of bacterial infections in humans and animals.
The present invention is also directed to a method of treating a subject
having a condition caused by or contributed to by bacterial infection, which
comprises administering to said mammal a therapeutically effective amount of
the compound of Formula I.
The present invention is further directed to a method of preventing a
subject from suffering from a condition caused by or contributed to by
bacterial infection, which comprises administering to the subject a

prophylactically effective dose of the pharmaceutical composition of a
compound of Formula I.
Other objects and advantages will become apparent to those skilled in
the art from a review of the ensuing specification.
prophylactically effective dose of the pharmaceutical composition of a
compound of Formula I.
Other objects and advantages will become apparent to those skilled in
the art from a review of the ensuing specification.
DETAILED DESCRIPTION
Relative to the above description of the phenyl oxazolidinone
compounds of the present invention, the following definitions apply.
Unless specified otherwise, the terms "alkyl", "alkenyl", and "alkynyl"
may be straight or branched groups with 1-8 carbon atoms.
"Acyl" means an organic radical having the designated number of
carbon atoms, derived from an organic acid by the removal of a hydroxyl
group having the formula RCO, as in the case of acetyl where R is CH3.
"Aryl" is an unsubstituted carbocyclic aromatic group including, but not
limited to, phenyl, 1- or 2-naphthyl and the like. "Heteroaryl" refers to a cyclic
aromatic radical having from five to ten atoms in the ring; where one to three
ring atoms are independent heteroatoms such as S, O, and N, and the
remaining ring atoms are carbon, for example, a pyridinyl, pyrazinyl,
pyrimidinyl, pyrroyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isoxazolyl,
thiadiazolyl, oxadiazolyt, thienyl, furanyl, quinolinyl, or isoquinolinyl, radical
and the like.
"Substituted aryl" or "substituted heteroaryl" refers to an aryl or
heteroaryl substituted by independent replacement of 1-3 of the hydrogen
atoms thereon with halogen, OH, CN, mercapto, nitro, C1-8-alkyl, halo-C-1-8-
alkyl, C-1-8-alkoxy, thio-C1-8-alkyl, amino, C1-8-alkyl-amine, di(C1-C8-alkyl-
)amino, formyl, carboxy, alkoxycarbonyl, C1-8-alkyl-CO-O-, C1-8-alkyl-CO-NH-,
or carboxamide. Further, substituted-heteroaryl may be substituted with a
mono-oxo to give, for example, a 4-oxo-1-H-quinoline. Substituted-heteroaryl
may also be substituted with a substituted-aryl or a second substituted-
heteroaryl to give, for example, a 4-phenyi-imidazol-1-yl or a 3-pyridinyl-
imidazol-1-yl, and the like.
The term "halo" or "halogen" means fluoro, chloro, bromo and iodo.
(mono-, di-, tri-, and per-) halo-alkyl is an alkyl radical substituted by
independent replacement of the hydrogen atoms thereon with halogen. P
denotes phosphorus.
The compounds of the instant invention are asymmetric in the
oxazolidinone ring at the 5- position and thus exist as optical antipodes. As
such, ail possible optical antipodes, enantiomers or diastereomers resulting
from additional asymmetric centers that may exist in optical antipodes,
racemates and racemic mixtures thereof are also part of this invention. The
antipodes can be separated by methods known to those skilled in the art such
as, for example, fractional recrystallization of diastereomeric salts of
enantiomerically pure acids. Alternatively, the antipodes can be separated by
chromatography on a Pirkle column.
The phrase "pharmaceutically acceptable salts" denotes salts of the
free base which possess the desired pharmacological activity of the free base
and which are neither biologically nor otherwise undesirable. These salts may
be derived from inorganic or organic acids. Examples of inorganic acids are
hydrochloric acid, nitric acid, hydrobromic acid, sulfuric acid, or phosphoric
acid. Examples of organic acids are acetic acid, propionic acid, glycolic acid,
lactic acid, pyruvic acid, malonic acid, succinic acid, malic acid, maleic acid,
fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, rnandelic
acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid,
methyi sulfonic acid, saiicyclic acid and the like. Suitable salts are
furthermore those of inorganic or organic bases, such as KOH, NaOH,
Ca(OH)2, AI(OH)3, piperidine, morpholine, ethylamine, triethylamine and the
like.
Also included within the scope of the invention are the hydrated forms
of the compounds which contain various amounts of water, for instance, the
hydrate, hemihydrate and sesquihydrate forms.
The term "subject" includes, without limitation, any animal or artificially
modified animal. In the preferred embodiment, the subject is a human.
The term "drug-resistant" or "drug-resistance" refers to the
characteristics of a microbe to survive in presence of a currently available
antimicrobial agent at its routine, effective concentration.
The compounds of the present invention possess antibacterial activity
against Gram-positive and certain Gram-negative bacteria. They are useful
as antibacterial agents for the treatment of bacterial infections in humans and
animals. Particularly, these compounds have antimicrobial activity against S.
aureus, S. epidermidis, S. pneumoniae, E. faecalis, E. faecium, Moraxelia
catarrhalis, and H. influenzae. More particularly, these compounds are useful
against resistant bacteria such as MRSA and GISA, and have a low
susceptibility to acquired resistance mechanisms. Compounds of Formula I
which are most preferred for such purposes are those in which R is any of the
following:
In addition Compounds of Formula I which are most preferred for such
purposes are those in which Y is any of the following:
Particular examples of the present invention include the following
compounds:
N-[[(5S)-3-[4-(1,3-Dihydro-2H-isoindol-2-yl)-3-fluorophenyl]-2-oxo-5-
oxazolidinyl]methyl]acetamide;
N-[[(5S)-3-[4-(1,3-Dihydro-pyrrolo[3,4-c]pyridin-2-yl)-3-
fluorophenyl]-2-oxo-5-oxazolidinyl]methyl]acetamide;
N-[[(5S)-3-[3-Fluoro-4-(5-oxido-2H-pyrrolo[3,4-clpyridin-2-yl)phenyl]-2-
oxo-5-oxazolidinyl]methyl]acetamide;
N-[[(5S)-3-[4-(5,7-dihydro-6H-pyrrolo[3.4-b]pyridin-6-yl)-3-fluorophenyl]-
2-oxo-5-oxazolidinyl]methyl]acetamide;
N-[(5S)-3-[4-(1,3-dihydro-1-oxo-2H-isoindol-2-yl)-3-fluorophenyl]-2-
oxo-5-oxazolidinyl]methyl]acetamide; and
(5R)-3-[4-(5,7-Dihydro-6H-pyrrolo[3,4-b]pyridin-6-yl)-3-fluorophenyl]-
5-(hydroxymethyl)-2-oxazolidinone.
The compounds of Formula I that are the subject of this invention may be
prepared from readily available starting materials such as isoindole (Gawley
et al., J. Org. Chem., 1988, 53:5381), 6,7-dihydro-5H-pyrrolo[3,4-c]pyridine
and 6,7-dihydro-5H-pyrrolo[3,4-b]pyridine (US Pat. No. 5,371,090 to Petersen
et al.) in accordance with synthetic methods well known in the art.
Representative proceduresare outlined in Scheme I-V:
In accordance with Scheme I, bicyclic heterocycles of general formula IV are
treated with a substituted nitrobenzene derivative (L is an appropriate leaving
group such as a halogen of trifluoromethanesulfonyloxy) in a suitable base
and solvent, such as diisopropylamine and ethyl acetate, to give the
substituted nitrophenyl compound V.
The nitrobenzene derivative V is then reduced to the aniline by an
appropriate reaction, for instance by treatment with SnCl2 or by catalytic
hydrogenation in the presence of a suitable catalyst, such as palladium on
carbon. The aniline is then treated with benzyl or methyl chloroformate and
sodium bicarbonate to form the corresponding benzyl or methyl carbamate
derivative VI.
The Cbz aniline VI is then deprotonated with a lithium base such as n-
butyllithium and reacted with (R)- glycidyl butyrate to afford the oxazolidinone
VII. The hydroxymethyl group can then be converted to an amide as shown in
Scheme I by preparation of the mesylate, conversion to azide VIII, and
reduction to amine IX by an appropriate procedure such as hydrogenation.
Alternatively displacement of a mesylate (Scheme II) or appropiate leaving
group such as tosylate or chlorine with potassium phthalimide and removal of
the phthaloyl protecting group by hydrazinolysis would provide amine IX. The
amine IX can be converted to amide X by an acylation reaction using
techniques known in the art, such as treatment with acetic anhydride in the
presence of a base such as pyridine. Alternatively, amine IX can be
converted to a carbamate XI by treatment with methylchloroformate and
pyridine, or reacted with a sulfonyl chloride in an inert solvent in the presence
of an organic base like pyridine to form a sulfonamide XII
For the formation of oxazolidione in which R = O-Heteroaryl (XIII), the
oxazolidinone carbinol VII can be converted to the corresponding mesylate or
other appropriate leaving group and reacted with HO-Het (a suitible hydroxyl
containing heterocycle), either in the presence of base or with HO-Het as a
preformed alkoxide, in an appropriate solvent, for example DMF or acetonitrile
(Scheme III). Alternatively, Mitsunobu conditions can be used to couple VII
with HO-Heterocycle by treating with triphenylphosphine and diisopropyl
azodicarboxylate (DIAD) in an appropriate solvent, such as THF, at a suitable
temperature, preferably room temperature. Reaction conditions and leading
references can be found in Gravestock et at, WO99/64416.
Furthermore, by treating VII with a suitable, non-nucleophilic base, for
example NaH, the displacement of a leaving group (LG), such as chlorine or
bromine, can be effected from an appropriately reactive aza-heterocycle (LG-
Het)(Scheme III).
Compounds of structure XIV can be prepared as shown in Scheme IV.
Amine IX can be converted to various functionalized amidines by reaction with
activated imines, where Q is a leaving group such as methylthio or methoxy,
in a suitable solvent, for example toluene or methanol, with or without a
catalyst (such AgNO3) present at a temperature range of 0-110ºC.
In accordance with Scheme V pyrrolidinone XV (prepared as in WO96/13502)
is first reacted with methoxy-bis(dimethylamine) or other activated
dimethylformamide reagent and, second, heated in a suitable solvent (for
example DMF and benzene) with either substituted amidines, to form
pyrrolopyrimidines oxazolidinones such as XVI, or substituted hydrazines, to
form pyrrolopyrazole oxazolidinones such as XVII. Formation of the
enamine, alkoxymethylene or alkoxycarbonyl derivatives of pyrrolidinone XV,
according to Brighty et al in US 5037834A, would also allow access to these
systems.
Scheme VI
As shown in Scheme VI compounds with the structure XIX can be
achieved by oxidation of the various compounds, XVIII, using an appropriate
oxidant (for example manganese dioxide, peroxyacetic acid, DDQ or air) in a
suitable solvent such as methylene chloride.
Oxo-derivatives of structure XXII in Scheme VII, (X = O, Y = H2 or X =
H2, Y = O) can be constructed by reacting 1,2-aryl dicarboxaldehydes (where
XXI, U = H) with aniline XX (prepared as in WO96/23788) in the presence of
acids, such as acetic acid, in a suitable solvent such as methylene chloride.
The di-oxo-derivatives ( structure XXII where X = Y = O) are prepared from
the reaction of aniline XX with selected 1,2-aryl dicarbonyl reagents with a
suitable leaving group (XXI where U = CI, Br, etc).
Definitions
All temperatures are in degrees Centigrade
Brine refers to an aqueous saturated sodium chloride solution
DMF refers to N,N-dimethylformamide
THF refers to tetrahydrofuran
Cbz refers to carbobenzyloxy
n-BuLi refers to n-butyl lithium
MS refers to mass spectrometry expressed as m/e or mass/charge unit
[M + H] refers to the positive ion of a parent plus a hydrogen atom
Ether refers to diethyl ether
rt refers to room temperature
Mp refers to melting point
CH2CI2 refers to methylene chloride
NaOH refers to sodium hydroxide
MeOH refers to methanol
EtOAc refers to ethyl acetate
ppt refers to a precipitate
These compounds have antimicrobial activity against susceptible and
drug resistant bacterial pathogens such as S. aureus, S. epidermidis, S.
pneumoniae, S. pyogenes, Enterococcus spp., Moraxella catarrhalis and H.
influenzae. These compounds are particularly useful against drug resistant
Gram-positive cocci such as methicillin-resistant S. aureus and vancomycin-
resistant enterococci. These compounds are useful in the treatment of
community-acquired pneumonia, upper and lower respiratory tract infections,
skin and soft tissue infections, hospital-acquired lung infections, bone and
joint infections, and other bacterial infections.
Minimal inhibitory concentration (MIC) has been an indicator of in vitro
antibacterial activity widely used in the art. The in vitro antimicrobial activity of
the compounds was determined by the microdilution broth method following
the test method from the National Committee for Laboratory Standards
(NCCLS). This method is described in the NCCLS Document M7-A4, Vol.17,
N0.2, "Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that
Grow Aerobically-Fourth Edition", which is incorporated herein by reference.

In this method twofold serial dilutions of drug in cation adjusted
Mueller-Hinton broth are added to wells in microdilution trays. The test
organisms are prepared by adjusting the turbidity of actively growing broth
cultures so that the final concentration of test organism after it is added to the
wells is approximately 5x104 CFU/well.
Following inoculation of the microdilution trays, the trays are incubated
at 35ºC for 16-20 hours and then read. The MIC is the lowest concentration of
test compound that completely inhibits growth of the test organism. The
amount of growth in the wells containing the test compound is compared with
the amount of growth in the growth-control wells (no test compound) used in
each tray. As set forth in Table 1, some compounds of the present invention
were tested against a variety of pathogenic bacteria resulting in a range of
activities, from 1 to >128 µg/mL depending on the organism tested. S. aureus
OC2878 is a MRSA and E. faecium OC3312 is a vancomycin resistant
enterococcus.
This invention further provides a method of treating bacterial infections,
or enhancing or potentiating the activity of other antibacterial agents, in a
subject having conditions caused by or contributed to by bacterial infection,
which comprises administering to the animals a compound of the invention
alone or in admixture with another antibacterial agent in the form of a
medicament according to the invention. The terms of "treating" and
"treatment" include administering, either simultaneously, separately or
sequentially, a pharmaceutically effective amount of a composition containing
one or more of the compounds disclosed herein to a subject that desires
inhibition of bacterial growth. The pharmaceutically effective amount of the
compound used to practice the present invention for treatment varies
depending on the manner of administration, the age, weight, and general
health of the subject treated, and ultimately will be decided by physicians or
veterinarians.
The compounds of the present invention may be administered to a
subject such as a human by any route appropriate to the condition to be
treated, suitable routes including oral, rectal, nasal, topical (including buccal
and sublingual), vaginal and parenteral (including subcutaneous,
intramuscular, intravenous, intradermal, intrathecal and epidural). The
preferred route may vary with, for example, the condition of the recipient as
well as the ease of preparation and administration.
When the compounds are employed for the above utility, they may be
combined with one or more pharmaceutically acceptable carriers, e.g.,
solvents, diluents, and the like, and may be administered orally in such forms
as tablets, capsules, dispersible powders, granules, or suspensions
containing for example, from about 0.5% to 5% of suspending agent, syrups
containing, for example, from about 10% to 50% of sugar, and elixirs
containing, for example, from about 20% to 50% ethanol, and the like, or
parenterally in the form of sterile injectable solutions or suspensions
containing from about 0.5% to 5% suspending agent in an isotonic medium.
These pharmaceutical preparations may contain, for example, from about
0.5% up to about 90% of the active ingredient in combination with the carrier,
more usually between 5% and 60% by weight.
Compositions for topical application may take the form of liquids,
creams or gels, containing a therapeutically effective concentration of a
compound of the invention admixed with a dermatologically acceptable
carrier.
In preparing the compositions in oral dosage form, any of the usual
pharmaceutical media may be employed. Solid carriers include starch,
lactose, dicalcium phosphate, microcrystalline cellulose, sucrose and kaolin,
while liquid carriers include sterile water, polyethylene glycols, non-ionic
surfactants and edible oils such as corn, peanut and sesame oils, as are
appropriate to the nature of the active ingredient and the particular form of
administration desired. Adjuvants customarily employed in the preparation of
pharmaceutical compositions may be advantageously included, such as
flavoring agents, coloring agents, preserving agents, and antioxidants, for
example, vitamin E, ascorbic acid, BHT and BHA.
The preferred pharmaceutical compositions from the standpoint of
ease of preparation and administration are solid compositions, particularly
tablets and hard-filled or liquid-filled capsules. Oral administration of the
compounds is preferred. These active compounds may also be administered
parenterally or intraperitoneally. Solutions or suspensions of these active
compounds as a free base or pharmacological acceptable salt can be
prepared in water suitably mixed with a surfactant such as hydroxypropyl-
cellulose. Dispersions can also be prepared in glycerol, liquid polyethylene
glycols and mixtures thereof in oils. Under ordinary conditions of storage and
use, these preparations may contain a preservative to prevent the growth of
microorganisms.
The pharmaceutical forms suitable for injectable use include sterile
aqueous solutions or dispersions and sterile powders for the extemporaneous
preparation of sterile injectable solutions or dispersions. In all cases, the form
must be sterile and must be fluid to the extent that easy syringability exists. It
must be stable under the conditions of manufacture and storage and must be
preserved against the contaminating action of microorganisms such as
bacteria and fungi. The carrier can be a solvent or dispersion medium
containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol
and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.
The effective dosage of active ingredient employed may vary
depending on the particular compound employed, the mode of administration
and the severity of the condition being treated. However, in general,
satisfactory results are obtained when the compounds of the invention are
administered at a daily dosage of from about 0.1 mg/kg to about 400 mg/kg of
animal body weight, preferably given in divided doses two to four times a day,
or in sustained release form. For most large mammals the total daily dosage
is from about 0.07 g to 7.0 g, preferably from about 100 mg to 1000 mg.
Dosage forms suitable for internal use comprise from about 100 mg to 500 mg
of the active compound in intimate admixture with a solid or liquid
pharmaceutically acceptable carrier. This dosage regimen may be adjusted
to provide the optimal therapeutic response. For example, several divided
doses may be administered daily or the dose may be proportionally reduced
as indicated by the exigencies of the therapeutic situation.
The production of the above-mentioned pharmaceutical compositions
and medicaments is carried out by any method known in the art, for example,
by mixing the active ingredients(s) with the diluent(s) to form a pharmaceutical
composition (e.g. a granulate) and then forming the composition into the
medicament (e.g. tablets).

The following examples describe in detail the chemical synthesis of
representative compounds of the present invention. The procedures are
illustrations, and the invention should not be construed as being limited by
chemical reactions and conditions they express. No attempt has been made
to optimize the yields obtained in these reactions, and it would be obvious to
one skilled in the art that variations in reaction times, temperatures, solvents,
and/or reagents could increase the yields.

Isoindoline was synthesized employing the method of R. E. Gawley, S. R.
Chemburkar, A. L. Smith, T. V. Anklekar J. Org. Chem. 1988, 53, 5381.

To 3,4-difluoronitrobenzene (3.02 mL, 27.3 mmols) in ethyl acetate at rt was
added diisopropylethylamine (5.03 mL, 28.9 mmols) and then isoindoline
(3.50 g, 29.4 mmols) and stirred overnight. A yellow precipitate (ppt) formed
and was collected on a filter, washed with water and ether and dried in a
vacuum oven (30°C) to provide the product as a bright yellow solid (6.69 g,
95% yield). Mp = 200-202°C. MS (M + 1) = 327 m/z.
To the above nitro compound (2.62 g, 10.2 mmols) in ethanol (100 mL) was
added SnCI2 (9.84 g, 50.9mmols) and was refluxed for 16hrs. After cooling to
rt the reaction mixture was added to 10% aq. NaOH (300 mL) and extracted
with CH2CI2 (6x50 mL). The combined organic washings were washed with
brine (100 mL), dried over Na2SO4 and concentrated to give 2.63 g of an olive
green solid (aniline), which was used without further purification. To this
aniline in acetone (150 mL) and water (20 mL) was added NaHCO3 (1.84 g,
21.9 mmols) and then benzylchloroformate (1.68 mL, 11.8 mmols). After
stirring overnight the mixture was poured into ice water (100 mL) and the
resulting tan precipitate was collected on a filter, washed with water and dried
in a vacuum to give the Cbz aniline as a tan solid (3.50 g, 95% yield). Mp =
146-148°C. MS (M + 1) = 363 m/z.
Step 3:
To the above Cbz aniline (0.74 g, 2.04 mmols) in THF (10 mL) at -78ºC was
added n-BuLi (2.5 M, 0.82 mL, 2.05 mmols) dropwise. After stirring for 40
min, (R)-glycidyl butyrate (0.31 mL, 2.10 mmols) in THF (0.5 mL) was added
dropwise and the resulting mixture was allowed to warm to RT overnight. A
white precipitate had formed and was collected on a filter and washed with
water and ether. Chromatography on silica gel with 25% ethyl acetate/hexane
as eluent provided the product as a white solid (0.58 g, 87% yield). MS (M +
1) = 329 m/z.
To the oxazolidinone carbinol from Example 1 (0.58 g, 1.78 mmols), in DMF
(10 mL) and acetonitrile (10 mL) at 0°C was added triethylamine (0.74 mL,
5.31 mmols) and, after 10 min, methanesulfonyl chloride (0.2:8 mL, 3.62
mmols). After allowing the reaction mixture to warm to RT over an hour
starting material was still present so cooling and addition of tniethyl amine
(0.37 mL, 2.65 mmols) and methanesulfonyl chloride (0.14 mL, 1.81 mmols)
was repeated. The mixture was poured into water (50 mL) and extracted with
CH2CI2 (6 X 20 mL), washed with brine (4 x 10mL), dried over Na2SO4,
concentrated to afford the crude product as a brown oil (0.95 g). MS (M + 1)
= 407 m/z.
To the mesylate from Example 2 (0.95 g, 1.78 mmols) in DMF (25 mL) was
added sodium azide (0.47 g, 7.23 mmols) and heated to 70ºC for 16 hrs. After
cooling to rt water was added and the mixture extracted with ethyl acetate
(6X25 mL), washed with brine (4x10 mL), dried over Na2SO4, concentrated to
give 0.48 g of a tan solid. MS (M + 1) = 354 m/z.
Compound 1
The azide from Example 3 in ethyl acetate (25 mL) was placed in a Paar flask
and nitrogen bubbled through for 15 min whereupon 10% Pd/C (0.15 g, 0.14
mmol) was added. The mixture was pressurized with 50 psi of H2 (g) and
shaken for 16 hrs whereupon an additional amount of 10% Pd/C (0.15 g, 1.4
mmols) was added and the mixture shaken for an additional 6 hrs (at this
point MS (M + 1) = 328 mlz). After placing the mixture under nitrogen,
pyridine (0.22 mL, 2.72 mmol) and then Ac2O (0.51 mL, 5.30 mmol) were
added and the mixture strred for 2 hrs. The mixture was filtered through
celite, washing with ethyl acetate (100 mL), concentrated, and
chromatographed on silica (gradient elution 1%-5% MeOH/CH2Cl2) and then
triturated with ethyl acetate (3X3 mL) to give 0.19 g of a white solid
(Compound 1, 29% yield for 4 steps). Mp = 240-242°C. MS (M + 1) = 370
m/z.
Example 5
6.7-Dihydro-6-(2-fluoro-4-nitrophenvl)-5H-pyrrolo[3.4-b]pyridine: To 6,7-
dihydro-5H-pyrrolo[3,4-b]pyridine dihydrochloride salt (as described by
Petersen, et al. (Bayer) EP0520277A2)(42.8 g, 222 mmols) in DMF (1.2 L)
was added 2,4-difluoronitrobenzene (25 mL, 224 mmols). The mixture was
heated to 60°C and DIPEA (195 mL, 1.12 mols) was added dropwise from an
addition funnel over 2 hrs. After heating overnight the reaction mixture was
cooled to rt, poured into water (3 L), filtered and dried in a vacuum oven
(50ºC) to provide a yellow-green solid (53.8 g, 94% yield). MS (M + 1) = 260
m/z.
Step 2:
6,7-Dihydro-6-(2-fluoro-4-aminophenyl)-5H-pyrrolo[3,4-b]pyricline
To the above nitro compound (53.8 g, 208 mmol) in THF (175 mL) and
methanol (600 mL) was added ammonium formate (59.0 g, 907 mmol).
Nitrogen was bubbled through the reaction for approximately 30 minutes
whereupon 10% Pd/C (2.20 g, 21 mmols) was added. After stirring overnight
at rt under an atmosphere of nitrogen the reaction mixture was filtered through
a pad of Celite, washing thoroughly with methanol (400 mL), and
concentrated to a volume of ca. 200 mL. Water (300 mL) was added and the
mixture extracted with ethyl acetate (5X200 mL). The combined organic
layers were washed with brine, dried (Na2SO4), filtered, and utilized directly in
the next step without further purification. MS (M + 1) = 230 m/z.

6,7-Dihydor-6-(2-fluoro-4-(Aminocarboxybenzyl)phenyl)-5H-pyrrolo[3,4-
b]pyridine The above aniline (~208 mmols) in acetone (1 L) and water (160
mL) was cooled to 0°C whereupon sodium bicarbonate (37.4 g, 445 mmols)
was added followed by the dropwise addition of benzylchloroformate (34.2
mL, 228 mmols). The reaction mixture was allowed to warm to room
temperature and stirred overnight whereupon a ppt formed. The reaction was
poured into ice water (2 L) and the resulting precipitate was collected by
o
filtration. The solid was washed with water and dried in a vacuum oven (50 C)
to afford the Cbz derivative (73.0 g, 97% yield) as a salmon colored powder.
MS (M + 1) = 364 m/z.
Step 4:
(Compound 2). The above Cbz derivative (40.8 g, 112 mmols) in THF (1 L)
was cooled to -78ºC under a nitrogen atmosphere. To this mixture was
added n-BuLi (2.5 M, 45.8 mL, 114.5 mmols) dropwise via syringe over fifteen
minutes. The reaction was warmed to room temperature and allowed to stir
for 45 minutes before again being cooled to -78ºC. At this point (R)-glycidyl
butyrate (17.2 mL, 117 mmols) was added and the reaction mixture allowed to
warm to rt overnight during which time a precipitate formed. The ppt was
collected, washed with several portions of ether (5X100 mL) and dried in a
vacuum oven (50ºC) to afford 40.6 g of the ether solvate of the lithium
alkoxide as a tan fluffy powder. This material was then washed with several
portions of water (4X200 mL) and dried in a vacuum oven (50ºC) to afford the
oxazolidinone alcohol (34.1 g, 92% yield) as a tan granular solid. Mp = 208-
212°C, decomp. MS (M + 1) = 330 m/z.
Example 6
Oxazolidinone Mesylate. The above oxazolidinone carbinol (from Example
4) (33.8 g, 103 mmols) was suspended in DMF (1.25 L, previously degassed
with nitrogen) at rt under a nitrogen atmosphere. Triethylamine (50 mL, 360
mmols) was added followed by the dropwise addition of methanesulfonyl
chloride (13.5 mL, 174 mmols). After stirring for 3 hrs the reaction mixture was
poured into water (200 mL) and methylene chloride (1 L) added. A ppt was
filtered off, washed with water (3X200 mL) and dried in a vac oven (50ºC) to
afford the mesylate as a tan solid (28.1 g, 67%). The organic layer was dried
(Na2SO4), filtered and evaporated to also afford the mesylate (11.7 g, 28%
yield) as a tan solid. Both where characterized with MS (M + 1) = 408 m/z.
Oxazolidinone Azide. The above mesylate (from Example 5) (27.8 g, 68.2
mmols) and sodium azide (17.7 g, 271 mmols) in anhydrous DMF (1 L),
previously degassed with nitrogen, were heated 95ºC for 6 hr under a nitrogen
atmosphere. After cooling, the mixture was poured into stirred ice water (2 L)
and formed a flocculant white ppt. The ppt was collected on a filter and
washed with water (4X200 mL), dried in a vac oven (50ºC) to afford the azide
as a light beige solid (22.7 g, 94% yield). Mp = 175-180 C, decomp. MS (M +
1) = 355m/z.
Example 8
Oxazolidinone Acetamide. The above azide (from Example 6)(21.67 g, 61.16
mmol) dissolved in DMF (400 mL) and THF (500 mL) was degassed with
nitrogen for 30 minutes whereupon 10% Pd/C (4.74 g, 4.4 mmols) was added
and the reaction hydrogenated on a Parr apparatus (60 psi of hydrogen) for
14 hr. The reaction mixture was removed from the Parr apparatus and placed
under a nitrogen atmosphere whereupon pyridine (5.44 mL, 67.3 mmols) and
acetic anhydride (6.35 mL, 67.3 mmols) were added. After stirring for 1 hr the
reaction mixture was filtered through a pad of Celite, washing thoroughly with
methanol and then copious amounts of 50% MeOH/CH2CI2 (ca. 2 L). The
filtrate was evaporated to afford the crude acetamide in DMF. The mixture
was slowly added to water (2 L) and the ppt collected on a filter, washed with
water (5X400 mL) and dried in a vac oven (50ºC) to provide the acetamide as
an analytically pure white solid (14.2 g, 63% yield). The combined filtrates
were extracted with methylene chloride (5X200 mL), dried over Na2SO4 and
concentrated. Water was added to the residue and the resulting ppt was
filtered off and dried in a vac oven (50ºC) to afford a second crop of the
acetamide as a light tan, fluffy solid (5.61 g, 25%). For the analytically pure
material Mp = 229-230ºC, decomp. MS (M + 1) = 371 m/z.
Example 9
The above acetamide from Example 8 (2.51 g, 6.78 mmols) was taken up in
CH2CI2 and MnO2 added (23.9 g, 234 mmols). After stirring overnight the
reaction mixture was filtered through celite, concentrated and chromatography
on silica with 10% MeOH/CH2Cl2 as eluent to afford the product as a light
yellow solid (0.48 g, 19% yield). Mp = 220-225°C decomp. MS (M + 1) = 369
m/z.
Example 10
Compound 5 was prepared as in Example 8 except (S)-glycidyl butyrate was
employed in the oxazolidinone formation. The product was isolated as a light
tan solid. Mp = 227-230°C decomp. MS (M + 1) = 371 m/z.
Example 11
Compound 6 was prepared as in Example 9 and isolated as a light yellow
solid. Mp = 181-185 C decomp. MS (M + 1) = 369 m/z.
To 5-hydroxyisoxazole (prepared as in Chem Pharm Bull 1966, 14(11), 1277)
(0.174 g, 2.04 mmols) in DMF was added NaH (60% in oil)(0.105 g, 2.62
mmols). After stirring for 30 min the mesylate (from Example 6) (0.744g, 1.82
mmols) was added in one portion and the mixture stirred at 60ºC overnight.
After cooling to rt water was added and a ppt was collected on a filter, air
dried and chromatographed on silica with 2.5% MeOH/CH2Cl2 as eluent to
afford the product as a white solid (0.140 g, 19 % yield). Mp = 182-185ºC.
MS (M + 1) = 397 m/z.
Example 13
To the above oxazolidinone (from Example 12) (0.264 g, 6.66 mmols) was
taken up in CH2CI2 and MnO2 added (1.66 g, 16.2 mmois) in two portions over
two days. After stirring for two days the reaction mixture was filtered through
celite, concentrated and chromatographed on silica with 10% MeOH/CH2Cl2
as eiuent to afford the product as a light yellow solid (0.086 g, 32% yield). Mp
= 133-135°C. MS (M + 1) = 395 m/z.
Compound 9
To NaH (60% by wt in oil)(0.03 g, 0.76 mmol) in DMF (5 mL) was added
oxazolidinone carbinol (from Example 5) (0.23 g, 0.71 mmol) in four portions.
After stirring for 30 min 2-chloropyrazine (0.065 mL, 0.71 mmol) was added
via syringe and stirred overnight at rt. Water was added and a ppt was
collected on a filter, air dried and chromatographed on silica with 5%
MeOH/CH2CI2 as eluent to afford the product as a white solid (0.067 g, 23 %
yield). Mp = 225-230'C. MS (M + 1) = 408 m/z.
The above oxazolidinone (from Example 14) (0.024 g, 0.058 mmol) in CH2CI2
(5 mL) was added MnO2 (0.07 g, 0.7 mmol). After stirring overnight the
reaction mixture was filtered through Celite and concentrated to afford the
product as a very light yellow solid (0.015 g, 64% yield). Mp = 192-194 C.
MS (M + 1) = 406 m/z.
Compound 11
To a suspension of the oxazolidinone carbinol (prepared in Example 5) (330
mg, 1.0 mmol), triphenylphosphine (260 mg, 1.1 mmols) and 4-hydroxy-1, 2,
5-thiadiazole (100 mg, 1.0 mmol) (as prepared in U.S Patent 3,391,150
[7/2/68]) in THF (8 mL) was added diisopropylazodicarboxylate (0.20 mL, 1.1
mmols). After stirring overnight at rt the reaction mixture was filtered, washed
with methanol, and air dried to afford a yellow crystalline solid (60 mg, 15%
yield). Mp = 185-187°C. MS (M + 1) = 414 m/z.
To the oxazolidinone (prepared in Example 16) (160 mg, 0.39 mmoi)
suspended in CH2CI2 (1.0 mL) was added MnO2 (four additions of 150 mg
over four days). The reaction mixture was filtered through a plug of Celite,
washed with CH2CI2 (15 mL), and concentrated under reduced pressure to
afford the product as a white crystalline solid (63 mg, 40% yield). Mp = 185-
188°C. MS(M + 1) = 412 m/z.
To the amine (as prepared in Example 8) (100 mg, 0.30 mmol) and potassium
carbonate (100 mg, 0.72 mmol) suspended in methanol (1.0 mL), was added
propionyl chloride (50 mg, 0.54 mmol). After stirring overnight at 80 C the
reaction mixture was cooled and water was added. A precipitate was filtered
off, washed with methanol and air dried to afford the product as a brown
crystalline solid (15 mg, 13 % yield). Mp = 110-112ºC. MS (M + 1) = 385 m/z.
To the amide (prepared in Example 18) (15 mg, 0.04 mmol) suspended in
CH2CI2 (1.0 mL), was added MnO2 (200 mg) at rt. After stirring overnight, the
reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10
mL), and concentrated under reduced pressure to afford the product as an
light brown crystalline solid (1.6 mg, 8 % yield). MS (M + 1) = 383 m/z.
To the amine (as prepared in Example 8) (60 mg, 0.18 mmol) and potassium
acetate (60 mg, 0.61 mmol) suspended in methanol (1.0 mL), was added
cyclopropyl carbonyl chloride (120 mg, 1.15 mmols). After stirring at rt
overnight, the reaction mixture was filtered, rinsed with methanol, and then
concentrated to dryness under reduced pressure. The resulting solid residue
was triturated with water and filtered to afford the product as a brown
crystalline solid (36 mg, 50 % yield). Mp = 235-240°C. MS (M + 1) = 397 m/z.
Example 21
To the amide (prepared in Example 20) (36 mg, 0.09 mmol) suspended in
CH2CI2 (1.0 mL), was added MnO2 (three portions of 100 mg over three days)
at rt. The reaction mixture was filtered through a plug of Celite, washed with
CH2CI2 (10 mL), and concentrated under reduced pressure to afford the
product as an off-white crystalline solid (3 mg, 8 % yield). MS (M + 1) = 395
m/z.
Example 22
To the amine (prepared in Example 8) (60 mg, 0.18 mmol) and potassium
acetate (60 mg, 0.61 mmol) suspended in methanol (1.0 mL), was added
dropwise methyl chloroformate (120 mg, 1.27 mmols). After stirring for four
hours at rt, the reaction mixture was filtered, diluted with water, and
concentrated under reduced pressure to remove the methanol. The aqueous
solution was extracted with ethyl acetate (5X5 mmL). The combined organics
were washed with water, dried over MgSO4, filtered, and concentrated to
provide an oil which was triturated with ether to afford a brown crystalline solid
(35 mg, 50% yield). MS (M + 1) = 387 m/z.
Example 23
To the carbamate (prepared in Example 22) (33 mg, 0.08 mmol) suspended in
CH2CI2 (1-0 mL), was added MnO2 (150 mg). After stirring overnight at rt the
reaction mixture was filtered through a plug of Celite, washed with CH2CI2 (10
mL), and concentrated under reduced pressure to afford the product as a
yellow crystalline solid (6.0 mg, 18% yield). MS (M + 1) = 385 m/z.
Example 24
Compound 19
To the amine (prepared in Example 8) (60 mg, 0.18 mmol) and potassium
acetate (60 mg, 0.61 mmol) suspended in methanol (1.0 mL) was added
dropwise ethyl chloroformate (0.1 mL, 1.04 mmols). After stirring overnight at
rt the reaction mixture was filtered, diluted with water, and concentrated under
reduced pressure to remove the methanol. The aqueous solution was
extracted with ethyl acetate (5X5 mL). The combined organics were washed
with water, dried over MgSO4, filtered, and concentrated. The resulting semi-
solid was treated with water, filtered and air-dried to afford a brown crystalline
soiid (18 mg, 30% yield). MS (M + 1) = 401 m/z.
To the amine (prepared in Example 8) (95 mg, 0.29 mmol) suspended in
pyridine (0.5 mL) was added methane sulfonylchloride (0.08 mL, 1.0 mmol).
After stirring overnight at rt the pyridine was removed under a stream of
nitrogen. The residue was treated with water, filtered and air-dried to afford a
brown solid (45 mg, 38% yield). Mp = 172-176°C. MS (M + 1) = 407 m/z.
To the sulfonamide (prepared in Example 25) (10 mg, 0.02 mmol) suspended
in CH2CI2 (1.0 mL), was added MnO2 (100 mg, 10 mmols). After stirring
overnight the reaction mixture was filtered through a plug of Celite, washed
with CH2CI2 (10 mL), and concentrated under reduced pressure to afford the
product as a brown crystalline solid (0.5 mg, 5% yield). MS (M + 1) = 405 m/z.
Compound 22
To the amine (prepared in Example 8) (200 mg, 0.61 mmol) suspended in
toluene (8 mL), was added dimethyl-N-cyanodithioiminocarbonate (89 mg,
0.61 mmol). After stirring overnight at reflux the toluene was decanted and
the oily residue treated with methanol, filtered, and air-dried to afford a brown
crystalline solid (62 mg, 20% yield). Mp = 204-207°C. MS (M + 1) = 427 m/z.
Compound 23
A suspension of the thioimidate (from Example 27) (45 mg, 0.10 mmol) and
MnO2 (200 mg, 2.0 mmols) in CH2CI2 were stirred at rt for one day whereupon
a second addition of MnO2 (150 mg, 1.5 mmols) was added. After an
additional day of stirring the mixture was filtered through Celite, washed with
CH2CI2 (10 mL), concentrated to afford a yellow crystalline solid (20 mg, 45%
yield). MS (M + 1) = 426 m/z.
A suspension of the amine (prepared in Example 8) (165 mg, 0.5 mmol) and
2-methyl-1-nitro-2-thiopseudourea (94 mg, 0.70 mmol) (as prepared as in EP
0539204/1993) in methanol (2 mL) was refluxed for four hours. After cooling
to rt the reaction mixture was filtered and air dried to afford a yellow crystalline
solid (50 mg, 24% yield). Mp = 202-206°C. MS (M + 1) = 416 m/z.
To the nitroguanidine (prepared in Example 29) (35 mg, 0.08 mmol)
suspended in CH2CI2 (1.0 mL) was added MnO2 (three additions of 100 mg
over three days). The reaction mixture was filtered through a plug of Celite,
washed with CH2CI2 (10 mL), and concentrated under reduced pressure to
afford the product as a yellow crystalline solid (1.6 mg, 4% yield). IMS (M + 1)
= 414 m/z.
The starting material 6,7-dihydro-5H-pyrrolo[3,4-c]pyridine was prepared as in
US Pat. No. 5,371,090 to Petersen et al. Compound 26 was then prepared as
in Example 8 except the acetamide was recrystallized from acetonitrile to give
a light tan solid. Mp = 182-190ºC decomposition. MS (M + 1) = 371 m/z.
Compound 27 was isolated from the final step of Example 31 via
chromatography (5% MeOH/CH2CI2 as eluent) of the mother liquors collected
from recrystallization. Light yellow solid, Mp = 219-225ºC decomp. MS (M +
1) = 385 m/z.
Compound 28 was prepared as in Example 9 except with 10% MeOH/CH2Cl2
as eluent. Light yellow solid, Mp = 219-225ºC decomposition. MS (M + 1) =
369 m/z.
Isothiazole (0.088 g, 0.87mmol)(prepared as in J Heterocyclic Chem 1971, 8,
591) was added portionwise at rt to a suspension of sodium hydride (0.036 g,
0.91 mmol, 60% in oil) in DMF (4 mL) under nitrogen. The mixture was stirred
for 30 minutes whereupon the mesylate from Example 31 (0.31 g, 0.76 mmol),
in DMF (10 mL), was added all at once. After stirring for 6 hours at 60ºC the
reaction mixture was cooled to rt, diluted with water (50 mL), and extracted
with ethyl acetate (3x50 mL). The combined organics were washed several
times with water, then once with brine, dried over sodium sulfate,
concentrated, and chromatographed on silica with 5% MeOH/EtOAc as
eluent. Two products were isolated from the chromatography: 0.050g of
Compound 29; and 0.022 g of Compound 30. Overall yield, 30%.
Compound 29 MS (M+1) = 413.0
Compound 30 MS (M+1) = 411.1
Example 35
To a suspension of sodium hydride (0.036 g, 0.91 mmol, 60% in oil) in DMF (4
mL) at rt under nitrogen was added portion wise 4-hydroxy-1, 2, 5-thiadiazole
(0.088 g, 0.87 mmol) (as prepared in U.S Patent 3,391,150 [7/2/68]). After
stirring for 30 min the mesylate from Example 31 (0.310 g, 0.76 mmol), in
DMF (10 mL), was added all at once. After stirring for 6 hours at 60 C the
reaction mixture was cooled to rt, diluted with water (50 mL), and extracted
with ethyl acetate (3x50 mL). The combined organics were washed several
times with water, then once with brine, dried over sodium sulfate,
concentrated, and chromatographed on silica with 2% MeOH/EtOAc as
eluent. Two products were isolated from the chromatography: 0.035 g of
Compound 31; and 0.0093 g of Compound 32. Overall yield, 14%.
Compound 31 MS (M+1 ) = 414.0
Compound 32 MS (M+1 ) = 412.1
To the mesylate from Example 31 (2.45 g, 6.01 mmol) dissolved in degassed
DMF (100 mL) under nitrogen was added potassium phthalimide (2.23 g, 12.0
mmols). After heating at 65ºC for 3 hours the reaction mixture was cooled,
poured into water (300 mL), and extracted with methylene chloride (3x200
mL). The combined organics were washed with water (3x150 mL) dried over
sodium sulfate, concentrated to a tan solid. This solid was washed with water
and dried in a high vacuum oven at 50ºC to afford 2.20 g (80%) of the
oxazolidinone phthalimide. MS= 459.1 (M+1)
Step 2:
To the above phthalimide (0.97 g, 2.1 mmols) in degassed methanol (30 mL)
under nitrogen was added hydrazine monohydrate (0.2 mL, 4.3 mmols)
dropwise. After refluxing for 12 hours the reaction mixture was cooled to rt,
and concentrated, suspended CH2CI2 and filtered. The crude oxazolidinone
amine was concentrated and used without further purification.
Step 3:
Compound 33,
To the crude amine (0.14 g, 0.44 mmol) in CH2CI2 (5 mL) was added pyridine
(0.14 mL, 18 mmols) followed by propionyl chloride (0.76 mL, 0.88 mmol).
After stirring for 5 hrs at rt the solution was poured into water (20 mL) and
extracted with methylene chloride (3x10 mL). The combined extracts were
washed with water (10 mL) and 1 M NaOH (aq) (10 mL), dried over sodium
sulfate, concentrated and chromatographed using neat EtOAc as eluent to
afford the propionyl amide as a gold oil (0.020 g, 12% yield). MS= 385.2
(M+1)
To the crude amine (as prepared in Example 36) (0.144 g, 0.437 mmol) in
methylene chloride (5 mL) was added pyridine (0.14 mL, 1.7 mmols), followed
by cyclopropane carbonyl chloride (0.08 mL, 0.88 mmol). After stirring for 5
hrs at rt the solution was poured into water (20 mL) and extracted with
methylene chloride (3x10 mL). The combined extracts were washed with
water (10 mL) and 1 M NaOH (aq) (10 mL), dried over sodium sulfate,
concentrated and chromatographed using a gradient elution of 1% to 5% to
10% MeOH/ EtOAc. The desired product eluted with 5% MeOH/ EtOAc and
was concentration to afford the product as a white powder (0.012 g, 7% yield).
MS= 397.2 (M+1)
To N-[(3-pyrrolidinone-3-fluorophenyl) 5-oxazolidinyl]methyl acetamide
(prepared according to W096/13502)(0.150 g, 0.447 mmols) was added
methoxy-bis(dimethylamino)methane (1 mL). After heating at 50 C for 15 min
the reaction mixture was concentrated to provide the crude ß-ketoenamine
which was used without further purification.
Step 2;
Compound 35
To ethanolic NaOEt (made from 0.027 g Na in 3 mL EtOH) was added
acetamidine hydrochloride (0.113 g, 1.19 mmols) and the above ß-
ketoenamine oxazolidinone acetamide. After refluxing for 3 hrs the reaction
mixture was cooled to rt, concentrated, taken up in chloroform, and washed
with water (3x8 mL). After drying over sodium sulfate the crude product was
concentrated, dissolved in 5% MeOH/ EtOAc, and filtered to afford the
product as an off-white solid (0.052 g, 45% yield). Mp = 234°C, decomp. MS
= 385.9 (M+1)
Example 39
To N-[(3-pyrrolidinone-3-fluorophenyl) 5-oxazolidinyi]methyl acetamide
(prepared according to W096/13502)(0.099 g, 0.29 mmol) was added
methoxy-bis(dimethylamino)methane (1.0 mL). After heating at 50ºC for 2 hrs
the reaction mixture was concentrated to provide the crude ß-ketoenamine.
To this mixture was added benzene (5 mL), DMF (1 mL) and formamidine
acetate (0.55 g, 5.3 mmols). After heating overnight at 95ºC the reaction
mixture was cooled to rt and water (8 mL) was added. A ppt formed and was
collected by filtration, dried in a vacuum oven (50ºC), and chromatographed
on silica with 5% MeOH/CH2CI2 as eluent to afford the product as a white
powder (0.037 g, 34% yield). Mp = 230-232°C. MS (M + 1) = 372 rn/z.
The above acetamide from Example 39 (0.020 mg, 0.054 mmol) was taken up
in CH2CI2 (5 mL) and MnO2 added (0.10 g, 0.98 mmol). After stirring
overnight at rt the reaction mixture was filtered through Celite and
concentrated to afford the product as a light yellow solid (0.016 g, 80% yield).
Mp = 164-166°C. MS (M + 1) = 370 m/z.
Example 41
To the ß-ketoenamine (prepared as in Example 39) was added benzene (5
mL), DMF (1 mL) and pyrazine-2-carboxamidine hydrochloride (0.62 g, 3.9
mmols). After heating overnight at 95ºC the reaction mixture was cooled to rt
and water (8 mL) was added. A ppt formed and was collected by filtration,
dried in a vacuum oven (50ºC), and chromatographed on silica with 5%
MeOH/CH2CI2 as eluent to afford the product as a light yellow solid (0.0026 g,
2% yield). Mp = 212-214°C. MS (M + 1) = 450 m/z.
The above acetamide from Example 39 (0.040 g, 0.088 mmols) was taken up
in CH2CI2 (10 mL) and MnO2 (0.36 g, 3.5 mmols) added in three portions over
three days. After stirring for three days the reaction mixture was filtered
through Celite, concentrated and chromatography on silica with 7%
MeOH/CH2Cl2 as eluent. Two products were isolated from the
chromatography: 0.001 g of Compound 39 as a light yellow solid (4% yield);
and 0.002 g of Compound 40 as a yellow solid (4% yield).
Compound 39: MS (M + 1) = 448 m/z.
Compound 40: MS (M + 1) = 464 m/z.
Example 43
Compound 41
To the ß-ketoenamine (prepared as in Example 39) was added benzene (5
mL), DMF (1 mL) and 4-amidinopyridine hydrochloride (0.81 g, 5.2 mmols).
After heating overnight at 95ºC the reaction mixture was cooled to rt and water
(8 mL) was added. A ppt formed and was collected by filtration, dried in a
vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2
as eluent to afford the product as a light yellow solid (0.072 g, 55% yield). Mp
= 245-250°C, decomp. MS (M + 1) = 449 m/z.
Example 44
Compound 42
To the ß-ketoenamine (prepared as in Example 39) was added benzene (5
mL), DMF (1 mL) and 2-amidinopyridine hydrochloride (0.61 g, 3.9 mmols).
After heating overnight at 95ºC the reaction mixture was cooled to rt and water
(8 mL) was added. A ppt formed and was collected by filtration, dried in a
vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2
as eluent to afford the product as a yellow powder (0.054 g, 40% yield). Mp =
216-220°C. MS (M + 1) = 449 m/z.
To the ß-ketoenamine (prepared as in Example 39) was added benzene (5
mL), DMF (2 mL) and 3-amidinopyridine hydrochloride (0.49 g, 3.1 mmols).
After heating overnight at 95ºC the reaction mixture was cooled to rt and water
(8 mL) was added. A ppt formed and was collected by filtration, dried in a
vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2Cl2
as eluent to afford the product as a light purple, crystalline solid (0.044 g, 33%
yield). Mp = 265-270°C, decomp. MS (M + 1) = 449 m/z.
To the ß-ketoenamine (prepared as in Example 39) was added benzene (5
mL), DMF (2 mL) and hydrazine hydrochloride (0.22 g, 3.2 mmols). After
heating overnight at 95ºC the reaction mixture was cooled to rt and water (8
mL) was added. A ppt formed and was collected by filtration, dried in a
vacuum oven (50ºC), and chromatographed on silica with 5% MeOH/CH2CI2
as eluent to afford the product as off-white powder (0.022 g, 21% yield). Mp =
244-247°C, decomp. MS (M + 1) = 360 m/z.
To the ß-ketoenamine (prepared as in Example 39) was added benzene (5
mL), DMF (2 mL) and n-propylhydrazine oxalate (0.87 g, 5.3 mmols). After
heating overnight at 95ºC the reaction mixture was cooled to rt and water (8
mL) was added. A ppt formed and was collected by filtration, dried in a
o
vacuum oven (50 C), and chromatographed on silica with 5% MeOH/CH2Cl2
as eluent to afford the product as a light yellow solid (0.081 g, 55% yield). Mp
= 204-208°C. MS (M + 1) = 402 m/z.
The starting material aniline (N-[[(5S)-3-(4-amino-3-fluorophenyl)-2-oxo-5-
oxazolidinyl]methyl]-acetamide) was prepared as in World Patent WO
96/23788. To phthalic dicarboxaldehyde (0.0522 g, 0.378 mmol) in
acetonitrile (1 mL) was added glacial acetic acid (0.05 mL, 0.87 mmol) and
then the above aniline (0.0955 g, 0.357 mmol) in acetonitrile (5 mL) dropwise.
After 4 hrs water (10 mL) was added and a precipitate was collected on a filter
and washed with water and ether to provide Compound 46 as a light green
solid (0.0655g, 48%). Mp = 211-214°C. MS (M + 1) = 384 m/z.
Example 49
To starting material aniline (N-[[(5S)-3-(4-amino-3-fluorophenyl)-2-oxo-5-
oxazolidinyl]methyl]-acetamide)(0.095 g, 0.36 mmol)(as prepared in World
Patent WO 96/23788) in CH2CI2 (5 mL) was added triethylamine (0.15 mL, 1.1
mmols) and phthaloyl dichloride (0.056 mL, 0.39 mmol). After stirring
overnight a solid was collected on a filter, washed with water (10 mL) and
dried in vacuum oven (50 C) to afford the product as a off-white solid (0.060,
42%). Mp = 240-242°C. MS (M + 1) = 398 m/z.
To starting material aniline (N-[[(5S)-3-(4-amino-3-fluorophenyl)-2-oxo-5-
oxazolidinyl]methyl]-acetamide)(0.20 g, 0.75 mmol)(as prepared in World
Patent WO 96/23788) in acetonitrile (5 mL) was added 2,3-pyndine
dicarboxaldehyde (0.10 g, 6.6 mmols) and glacial acetic acid (0.050 mL, 0.87
mmol). After stirring for 5hrs the reaction mixture was concentrated and
chromatographed on silica with 2.5% MeOH/CH2Cl2 as eluent to afford the two
products: 0.035 g of Compound 52 (12%) as a yellow solid; and 0.011 g of
Compound 53 (4%) as a yellow solid.
Compound 48: Mp = 230-232°C. MS (M + 1) = 385 m/z.
Compound 49: Mp = 207-209°C. MS (M + 1) = 385 m/z.
The invention has been described in detail with particular reference to
the above embodiments thereof. The above embodiments and examples are
given to illustrate the scope and spirit of the present invention. These
embodiments and examples will make apparent, to those skilled in the art,
other embodiments and examples. These other embodiments and examples
are within the contemplation of the present invention. It will be understood
that variations and modifications can be effected within the spirit and scope of
the invention; therefore, the instant invention should be limited only by the
appended claims.
We claim:
1. A compound of Formula I
wherein:
R is selected from the group consisting of OH, O-Aryl, O-
Heteroaryl, N3, OR, OSO2R", -NR"'R"", or

wherein:
(i) R1 is straight-chain or branched acy! having up to 6 carbon
atoms or benzyl;
(ii) R"is straight-chain or branched alkyl, having up to 5 carbon
atoms, phenyl or tolyl; and
(iii) R"' and R"" are independently selected from the group
consisting of H, cycloalkyl having 3 to 6 carbon atoms, phenyl or tert-
butoxycarbonyl, fluorenyloxycarbonyl, benzyloxycarbonyl, straight-
chain or branched alkyl having up to 6 carbon atoms which is
optionally substituted by cyano or alkoxycarbonyl having up to 4
carbon atoms, -CO2-R1, -CO-R1, -CS-R1, and -SO2-R4, In which
R1 is selected from the group consisting of H, cycloalkyl having 3 to 6
carbon atoms, trlfluoromethyl or phenyl, benzyl or acyl having up to
5 carbon atoms, straight-chain or branched alky! having up to 6
carbon atoms, said alkyl optionally substituted by straight-chain or
branched alkoxycarbonyl having up to 5 carbon atoms, OH, cyano,
up to 3 halogen atoms, and -NR6 R6 in which R5 and R6 are identical
or different and are selected from H, phenyl or straight-chain or
branched aikyl having up to 4 carbon atoms;
R4 is selected from straight-chain or branched aikyi having up to 4
carbon atoms or phenyl and;
R4a is CN, COR4c, COOR4a, CONHR4c, CO-NR4c, R4d, SO2R4c, or NO2;
R4b is H, alkyl, OR4c SR4c amino, NHR4c NR4c R4c;
R4c and R4d are independently selected from H, alkyl, aryl, or in the
case of any NR4c R4d, group R4c and R4d taken together with the
nitrogen atom to which they are attached form a unsubstituted or
substituted pyrrolidine piperidinyl or morphoiinyi group;
X is 0 to 4 members independently selected from the group
consisting of halogen, OH, nitro, C1-8 alkoxy, C1-8 alkyl-amino, di(C1-8-
alkyl-) amino, carboxy, alkoxycarbonyl, C1-8 alkyl-CO-O-, C1-8 alkyl-
CO-NH-, carboxamide , CN, amine, C3-6 cycloalkyl, C1-8 alkyl optionally
substituted with one or more members selected from the group
consisting of F, CI, OH; and
Y is a radical of Formulae II or III:
wherein
R5, R6, R7 and R8 are each Independently H, alkyl, CN, nltro, C1-8-
alkyl, formyl, carboxy, alkoxy-carbonyl, carboxamide, or R6 and R6
and/or R7 and R6 together form an oxo group;
R9, and R10 are each independently H, halogen, alkyl, OH, CN, nitro,
C1-8 alkyl, halo-C1-8-alkyl, C1-8 alkoxyl, amino, C1-8-alkyl-amino, di (C1-
8-alkyl-) amino, formyl, carboxyl, alkoxycarbonyl, C1-8-alkyl-CO-O-, C1-
8-alkyl-CO-NH-, carboxamide, or amine;
is a fused phenyl ring or a five-or six-membered heteroaromatlc ring
having one to four members selected from the group consisting of S,
0, and N;
Z is selected from halogen, alkyl, aryl, substltuted-aryl, heteroaryl,
substltuted-heteroaryl CN. CHO, Coalkyl, amine, (dlallkyiamino) alkyl
where dialkyiamlno is selected from dimethylamine, diethylamine,
morpholinyl, thiomorphollnyl, pyrroldlnyl, or plperidinyl, or, alkoxy, or
NHCO-CC1-C8-alkyl); and
m is 0 to 1, and the pharmaceutical acceptable salts and esters
thereof.
2. The compound as claimed in claim 1 wherein Y is selected from
the group consisting of
3. The compound as claimed in claim 1 wherein R is -NHCOCH3 or is
selectedfrom the group consisting of
4. A compound as claimed in claim 1 having the formula:
5. A pharmaceutical composition comprising a compoundas claimed
in any of claims 1 to 4 and a pharmaceutically acceptable carrier.
6. A compound as claimed in any one of claims 1 to 4 or a
composition as claimed in claim 5, for use in a method of treating
a subject having, or preventing a subject from suffering from a
condition caused by or contributed to by bacterial infection, such
as community-acquired pneumonia, upper or lower respiratory
tract Infection, skin or soft tissue Infection, bone or joint Infection
or a hospital acquired lung infection, such as caused by S. aureus,
S. epidermidls, S. pneumoniae, S. pyogenes, Entenococcus spp.,
Moraxeiia catarrhalis H influenzae, a Gram-positive coccus or a
Gram-positive coccus which is drug-resistant.
Bicycllc heterocyclic substituted phenyl oxazoildinone compounds of
formula (I): wherein Y is a radical of formulae (II) or (III) In which
the substituents have the meaning indicated in the description. These
compounds are useful as antibacterial agents.

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Patent Number

225465

Indian Patent Application Number

IN/PCT/2002/00141/KOL

PG Journal Number

46/2008

Publication Date

14-Nov-2008

Grant Date

12-Nov-2008

Date of Filing

29-Jan-2002

Name of Patentee

ORTHO-MCNEIL PHARMACEUTICAL, INC

Applicant Address

U.S. ROUTE NO. 202, P.O. BOX 300, RARITAN, NEW JERSEY

Inventors:

#	Inventor's Name	Inventor's Address
1	PAGET, STEVEN	2 CAMDON ROAD, BELLE MEAD, NJ 08502
2	HLASTA, DENNIS	5008 DAVIS DRIVE, DOYLESTOWN, NJ 18901

PCT International Classification Number

C07D 413/10, 471/04

PCT International Application Number

PCT/US00/21093

PCT International Filing date

2000-08-02

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	60/148,621	1999-08-12	U.S.A.