Title of Invention

A PROCESS FOR THE ISOLATION OF CRYSTALS OF OPTIONALLY SUBSTITUTED 2-(2-PYRIDINYL)METHYLTHIO-1H-BENZIMIDAZOLE HYDRATES

Abstract

"A PROCESS FOR THE ISOLATION OF CRYSTALS OF OPTIONALLY SUBSTITUTED 2-(2-PYRIDINYL)METHYLTHIO-1H-BENZIMIDAZOLE HYDRATES" A process for the isolation of crystals of optionally substituted 2-(2-pyridinyl)methylthio-lH-benzimidazole hydrates of the following structural formula I in which R1, R2 and R3, identical or different, denote hydrogen, a C1—C8 alkyl, C3-C8 cycloalkyl, C2-C8 fluoroalkyl or C1-C8 alkoxy, R4 and R5, identical or different, denote hydrogen, a C1-C8 alkyl, C3-C8 cycloalkyl, CH2- C3—C8 cycloalkyl, C1-C8 alkoxycarbonyl, C1-C8 alkoxy, C1-C8 fluoroalkoxy, CF3-, C2-C8 fluoroalkyl or C(O)O-C1-C8 alkyl and R6 denotes hydrogen or a C1-C2 alkyl and x means 0.5-2; wherein said process comprises isolation from a reaction medium as herein described containing the free base, characterized in that a water-soluble, organic solvent present in the reaction medium is at most partially removed, water is added to the reaction medium at a temperature of below 40°C water in quantities of at least 55 wt.%, relative to the reaction medium, and the hydrates formed are separated as crystals and optionally purified in conventional manner.

Full Text

FORM 2 THE PATENTS ACT 1970 [39 OF 1970] & THE PATENTS RULES, 2003 COMPLETE SPECIFICATION [See Section 10; rule 13] "A PROCESS FOR THE ISOLATION OF CRYSTALS OF OPTIONALLY SUBSTITUTED 2-(2-PYRIDINYL)METHYLTHIO- 1H-BENZIMIDAZOLE HYDRATES" GRUNENTHAL GMBH, a German company of Zieglerstrasse 6, 52078 Aachen, Germany, The following specification particularly describes the invention and the manner in which it is to be performed: The present invention relates to a process for the isolation of crystals of optionally substituted 2-(2-pyridinyl)methylthio-lh-benzimidazole hydrates. The invention relates to crystals of optionally substituted 2-(2-pyridinyl)methylthio-lH-benzimidazole hydrates and to a process for the production thereof. It is known that 2-(2-pyridinyl)methylthio-lH-benzimidazole compounds, such as for example pyrmetazole (5-methoxy-2-[(4-methoxy-3,5-dimethyl-2-pyridinyl)methylthio]-1H-benzimidazole) are the final intermediate for the production of antiulceratives, in particular omeprazole or lanzoprazole. Such an antiulcerative is produced by converting the sulfide compound, such as for example pyrmetazole, by oxidation into the corresponding sulfinyl compound, such as for example omeprazole. Substituted 2-(2-pyridinyl)methylthio-lH-benzimidazoles are conventionally produced under alkaline conditions in organic solvents by reaction of mercaptobenzimidazole compounds, such as for example 5-methoxy-2-mercapto-benzimidazole, with reactive pyridine compounds, such as for example 2-chloromethyl-3,5-dimethyl-4-methoxypyridine. EP 0 005 129 A describes the production of 2-(2-pyridinyl)methylthio-lH-benzimidazole compounds by reacting suitable mercaptobenzimidazole compounds with chloromethylpyridine compounds. The reaction proceeds in an organic solvent, such as for example ethanol, in the presence of a base, such as for example sodium hydroxide. Once the reaction is complete, the resultant common salt is separated, the solvent removed under a vacuum and the monohydrochloride of the compound is caused to crystallise by means of concentrated hydrochloric acid in acetone and is purified. The yields achieved in this process are barely satisfactory. Moreover, the hydrochloride must be converted back into the base before the oxidation reaction. EP 0 074 341 A describes the production of 2-(2-pyridinyl)-methylthio-lH-benzimidazole compounds by reacting suitable mercaptobenzimidazole compounds with chloromethylpyridine compounds in the presence of sodium hydroxide. Methanol is used as the solvent. Once the reaction is complete and water has been added, the 2-(2-pyridinyl)methylthio-lH-benzimidazole compound is purified by repeated extraction with methylene chloride and recrystallisation from acetonitrile. The solvents used in this process are hazardous to the environment. Moreover, due to the repeated extraction steps, the process is time-consuming. EP 0 899 268 A2 describes inter alia the production of 2-[2-(4-chloro-3,5-dimethylpyridyl)methylthio]-5-methoxy-1H-benzimidazole by the reaction of suitable starting compounds in tetrahydrofuran and in the presence of sodium hydroxide solution. Once the reaction is complete and water has been added, the stated compound is isolated by repeated extraction with methylene chloride and evaporation of the solvent. The compound is obtained as a viscous oil. Disadvantageous features of this process are the use of a solvent which is hazardous to the environment and the time-consuming isolation by repeated extraction. Moreover, the product in the form of a viscous oil is more technically demanding to handle than a crystalline compound. The object of the present invention was accordingly to provide the optionally substituted 2-(2-pyridinyl)-methylthio-lH-benzimidazoles in a form which is stable and can be stored and is obtained by straightforward processing steps in elevated yields and in high purity, wherein it is possible to use solvents which are more environmentally friendly and present a reduced hazard to health. The object is achieved by the provision of crystals of optionally substituted 2-(2-pyridinyl)methylthio-lH-benzimidazole hydrates of the structural formula I, in which R1, R2 and R3, identical or different, denote hydrogen, C1-C8 alkyl, C3-C8 cycloalkyl, C2-C8 fluoroalkyl or Cl-C8 alkoxy, R4 and R5, identical or different, denote hydrogen, C1-C8 alkyl, C3-C8 cycloalkyl, CH2-C3-C8 cycloalkyl, C1-C8 alkoxycarbonyl, C1-C8 alkoxy, C1-C8 fluoroalkoxy, CF3, C2-C8 fluoroalkyl or -C(O)O-C1-C8 alkyl and R6, identical or different, denotes hydrogen or C1-C2 alkyl and x denotes 0.5-2. R1 - R6 preferably have the following meaning R1, R2 and R3, identical or different, denote i hydrogen, C1-C3 alkyl or C1-C3 alkoxy, R4 and R5, identical or different, denote hydrogen, C1-C3 alkoxy, C1-C3 fluoroalkoxy and R6, identical or different, denotes hydrogen. Particularly preferred compounds are those in which R1 denotes a methyl group, R2 a methoxy group, R3 a methyl group, R4 hydrogen, R5 a methoxy group in position 5 and R6 hydrogen or in which R1 denotes hydrogen, R2 and R3 in each case denote a methoxy group, R4 denotes hydrogen, R5 a difluoromethoxy group in position 5 and R6 hydrogen. The present invention also provides a process for the isolation of a compound according to formula I from a reaction medium, in which the compound is present as a free base, in elevated yields, wherein a water-miscible, organic solvent present in the reaction medium is at most partially removed and water is added to the reaction medium at a temperature of below 40°C, preferably of 20-25°C, in quantities of at least 55 wt.%, preferably at least 70 wt.%, particularly preferably up to 75 wt.%, relative to the reaction medium, and the consequently formed hydrates are separated as crystals and optionally purified in conventional manner(and dried. When removing the organic solvent, care should be taken to ensure that its concentration does not fall below the solubility limit for the compound of the formula I. The compounds of the formula I are preferably to be separated from a reaction medium which is obtained by reacting a thiol compound of the formula II in which R4 and R5 have the above-stated meaning, with a reactive pyridine compound of the formula III, in which R1, R2, R3 and R6 have the above-stated meaning, in a water-miscible, organic solvent in the presence of a base. Suitable bases for this reaction are preferably sodium and/or potassium hydroxide. This reaction preferably proceeds with several hours' refluxing. The reaction may be performed continuously or discontinuously. The compound of the formula I may be obtained from the reaction medium, which contains the compound as a free base, by at most partial removal of the water-miscible, organic solvent and by addition of water in quantities of at least 55 wt.%, preferably at least 70 wt.%, particularly preferably up to 75 wt.%, relative to the reaction medium, at a temperature of below 40°C, preferably of 20-25°C. When removing the organic solvent, care should be taken to ensure that its concentration does not fall below the solubility limit for the compound of the formula I. The sodium and/or potassium chloride arising from the neutralisation of the added base may be separated beforehand by suitable means, for example by filtration, or be dissolved by the addition of water on formation of the hydrate. The hydrates of the 2-(2-pyridinyl)methylthio-lH-benzimidazoles may optionally be further purified and dried. Thiol compounds of the formula II may be purchased commercially. Reference is also made to EP 0 254 588, EP 0 005 129 and EP 0 074 341 for a description of the synthesis thereof, the corresponding description hereby being introduced as part of the present disclosure. Reference is made to WO 98/50361 and WO 97/29103 for a description of the synthesis of the reactive pyridine compounds of the formula III, the corresponding description hereby being introduced as part of the present disclosure. The present invention also provides a process for the production of a compound according to the formula I, in which the unhydrated compound of the formula I is dissolved in a water-miscible, organic solvent or solvent mixture and is caused to crystallise by addition of water in quantities of at least 55 wt.%, preferably at least 70 wt.%, particularly preferably up to 75 wt.%, relative to the reaction medium, at a temperature of below 40°C, preferably of 20-25°C, and the consequently formed crystals of the compound according to the formula I are separated, optionally purified and dried. The water-miscible, organic solvents used in the above-stated reactions are preferably highly volatile solvents, such as aliphatic alcohols, aprotic solvents or ketones, particularly preferably methanol, ethanol, propanol, butanol, dimethylformamide, dimethyl sulfoxide, tetrahydrofuran or acetone, or mixtures of at least two of these solvents. The present invention also provides a process for the purification of crystals of a compound according to the formula I, in accordance with which the hydrate to be purified is washed at least once with water and/or a solvent/water mixture, preferably an alcohol/water mixture and/or a ketone/water mixture, and is then dried under a vacuum at below the melting point of the hydrates. The crystals according to the invention of the optionally substituted 2-(2-pyridinyl)methylthio-lH-benzimidazole hydrates of the formula I are stable and can be stored. They are straightforward to produce, isolate and purify and may be used directly for oxidation to yield the corresponding sulfinyl compounds, which are used as an antiulcerative. With the assistance of the process according to the invention, it is possible to produce crystals of optionally substituted 2-(2-pyridinyl)-methylthio-1H-benzimidazole hydrates of the formula I in elevated yields and in high purity, wherein it is possible to use solvents which are more environmentally friendly and present a reduced hazard to health. The following Examples illustrate the invention without limiting it thereto. Examples Example 1 0.05 mol of 2-mercapto-5-methoxybenzimidazole were added to a solution of 0.11 mol of sodium hydroxide in 90 ml of ethanol. 0.05 mol of 2-chloromethyl-3,5-dimethyl-4-methoxy-pyridine hydrochloride were added to the solution and the reaction mixture was refluxed for 14 hours. 270 ml of water were then added at room temperature (25°C), wherein the hydrates of 5-methoxy-2-[3,5-dimethyl-4-methoxypyridinyl)-methylthio]-1H-benzimidazole crystallised. The whitish crystalline product was separated, washed with water and dried under a vacuum. Yield: 95% relative to theoretical yield (15.6 g). The purity of the compound was determined by HPLC and was 99.7%. Example 2 0.1 mol of 2-mercapto-5-methoxybenzimidazole were added to a solution of 0.22 mol of sodium hydroxide in 250 ml of methanol. 0.1 mol of 2-chloromethyl-3,5-dimethyl-4-methoxypyridine hydrochloride were added to the solution and the reaction mixture was refluxed for 16 hours. 80 ml of solvent were then removed under a vacuum and 400 ml of water were then added at room temperature (25°C), wherein the hydrates of 5-methoxy-2-[3,5-dimethyl-4-methoxy-pyridinyl)methylthio]-1H-benzimidazole crystallised. The whitish crystalline product was separated, washed with methanol/water and dried under a vacuum. Yield: 92.5% relative to theoretical yield (30.3 g). The purity of the compound was determined by HPLC and was 99.5%. Example 3 0.1 mol of pyrmetazole hydrochloride (the compound was produced according to details stated in Example 31 of EP 0 005 129) were dissolved in 60 ml of water, then 60 ml of ethanol were added, the pH value of the solution was adjusted to greater than pH 7 with a 5 N sodium hydroxide solution and a further 120 ml of water were added at room temperature (25°C), wherein the hydrates of the pyrmetazole crystallised. The whitish crystalline product was separated, washed with water and dried under a vacuum. Yield: 90.2% relative to theoretical yield (29.5 g). The purity of the compound was determined by HPLC and was 99.9%. Example 4 Pyrmetazole, in the form of a solution in methylene chloride, was first produced according to Example 26 of EP 0 899 268. Methylene chloride was removed under a vacuum from such a solution, which contained 0.1 mol of pyrmetazole. The residual oil was dissolved in 210 ml of ethanol and caused to crystallise as the hydrate of pyrmetazole by the addition of 630 ml of water. The whitish crystalline product was separated, washed with water and dried under a vacuum. Yield: 96% relative to theoretical yield (31.5 g). The purity of the compound was determined by HPLC and was 99.8%. WE CLAIM: 1. A process for the isolation of crystals of optionally substituted 2-(2-pyridinyl)methylthio-lH-benzimidazole hydrates of the following structural formula I in which R1, R2 and R3, identical or different, denote hydrogen, a C1—C8 alkyl, C3-C8 cycloalkyl, C2-C8 fluoroalkyl or C1-C8 alkoxy, R4 and R5, identical or different, denote hydrogen, a C1-C8 alkyl, C3-C8 cycloalkyl, CH2- C3—C8 cycloalkyl, C1-C8 alkoxycarbonyl, C1-C8 alkoxy, C1-C8 fluoroalkoxy, CF3-, C2-C8 fluoroalkyl or C(0)0-C1-C8 alkyl and R6 denotes hydrogen or a C1-C2 alkyl and x means 0.5-2; wherein said process comprises isolation from a reaction medium as herein described containing the free base, characterized in that a water-soluble, organic solvent present in the reaction medium is at most partially removed, water is added to the reaction medium at a temperature of below 40°C water in quantities of at least 55 wt.%, relative to the reaction medium, and the hydrates formed are separated as crystals and optionally purified in conventional manner. with a reactive pyridine compound of the formula III 2. A process as claimed in claim 1, wherein water is added in quantities of at least 70 wt.% relative to the reaction medium. 3. A process as claimed in claim 1, wherein water is added in quantities of up to 75 wt.% relative to the reaction medium. 4. A process as claimed in one of claims 1 to 3, wherein the water is added at a temperature of 20-25°C. 5. A process as claimed in one of claims 1 to 4, wherein an unhydrated compound of the formula I was obtained in the reaction medium by reacting a thiol-compound of the formula II in presence of at least one base, wherein the residues R1-R6 have the meaning stated in claim 1. 6. A process as claimed in claim 5, wherein sodium and/or potassium hydroxide was used as the base. 7. A process as claimed in one of claims 1 to 4, wherein the unhydrated compound of the formula. I was initially dissolved in a water-miscible, organic solvent. 8. A process as claimed in one of claims 1 to 7, wherein the water-miscible, organic solvent is an aliphatic alcohol, preferably methanol, ethanol, propanol or butanol, or an aprotic solvent, preferably dimethylformamide, dimethyl sulfoxide, tetrahydrofuran, or a ketone, preferably acetone, or a mixture of at least two these solvents. 9. A process as claimed in one of claims 1 to 8, wherein the crystals are purified by washing with water and/or a solvent/water mixture, preferably an alcohol/water mixture and/or a ketone/water mixture. dated this 02/11/2004. [ANUSHRI GUPTA] OF REMFRY & SAGAR ATTORNEY FOR THE APPLICANTS

Documents:

103-mumnp-2004-abstract(11-2-2004).pdf

103-MUMNP-2004-ABSTRACT(AMENDED)-(29-4-2008).pdf

103-mumnp-2004-abstract(granted)-(16-10-2008).pdf

103-mumnp-2004-cancelled pages(29-4-2008).pdf

103-MUMNP-2004-CANCELLED PAGES(29-4-2009).pdf

103-mumnp-2004-claims(11-2-2004).pdf

103-mumnp-2004-claims(granted)-(16-10-2008).pdf

103-MUMNP-2004-CORRESPONDENCE(13-5-2008).pdf

103-mumnp-2004-correspondence(29-4-2008).pdf

103-mumnp-2004-correspondence(ipo)-(14-11-2008).pdf

103-mumnp-2004-description(complete)-(11-2-2004).pdf

103-mumnp-2004-description(granted)-(16-10-2008).pdf

103-MUMNP-2004-FORM 1(11-2-2004).pdf

103-mumnp-2004-form 1(29-4-2008).pdf

103-mumnp-2004-form 13(29-4-2008).pdf

103-mumnp-2004-form 18(27-7-2006).pdf

103-MUMNP-2004-FORM 2(COMPLETE)-(11-2-2004).pdf

103-mumnp-2004-form 2(granted)-(16-10-2008).pdf

103-mumnp-2004-form 2(title page)-(11-2-2004).pdf

103-mumnp-2004-form 2(title page)-(granted)-(16-10-2008).pdf

103-mumnp-2004-form 3(1-6-2004).pdf

103-mumnp-2004-form 3(11-2-2004).pdf

103-mumnp-2004-form 3(15-4-2004).pdf

103-mumnp-2004-form 3(29-4-2008).pdf

103-mumnp-2004-form 3(6-9-2007).pdf

103-mumnp-2004-form 5(11-2-2004).pdf

103-mumnp-2004-petition under rule 137(29-4-2008).pdf

103-mumnp-2004-petition under rule 138(29-4-2008).pdf

103-mumnp-2004-power of authority(29-4-2008).pdf

103-MUMNP-2004-SPECIFICATION(AMENDED)-(29-4-2008).pdf

103-MUMNP-2004-SPECIFICATION(AMENDED)-(29-4-2009).pdf

103-mumnp-2004-wo international publication report(11-2-2004).pdf