Title of Invention

"ANTI-MICROBIAL COMPOSITION"

Abstract

Cystcin and/or cystein derivatives are used to combat bioslimes in liquid-conveying systems of technical installations. These substances are advantageously used in the process of tie invention, which consists essentially in adding cystein and/or a cystein derivative and a microbicidaj active substance, chosen from the group of aldehydic active substances, quaternary ammonium compounds, phenolic active substances, hothiazolinones and mixtures thereof, to the liquid medium of the liquid-conveying system so that effective concentrations of the active substances are present in the anti-fouling liquid. This is achieved by adding the individual components in mete. -3 quantities or advantageously by adding the agent of the invention, which contains a combination of cystein and/or cystein derivatives with a suitable microbicidal active substance.

Full Text

ANTI-MTCROBIAL COMPOSITION This invention relates to anti-microbial compositions and to formulations including the anti-microbial compositions. Microorganisms can be found in many environments and are known to present health hazards due to infection or contamination. When microorganisms are present on the surface of a substrate they can replicate rapidly to form colonies. Virtually all microorganisms replicate in this way. The microbial colonies form a coating, which is known as a biofilm, on the substrate surface. Biofilms are more hazardous to health than individual microorganisms. Some microorganisms also produce polysaccharide coatings, which makes them more difficult to destroy. A biofilm can be formed by a single bacterial species but mere often biofilms consist of several species of bacteria, as well as fungi, algae, protozoa, debris and corrosion products. Essentially, biofilm may form on any surface exposed to bacteria and some amount of water, which is needed to allow metabolic processes. Biofilm formation occurs via three distinct stages. The three stages are (i) adhesion or attachment, (ii) proliferation and (iii) biofilm differentiation. Before stage (i) can occur, the microorganisms must be transported to a surface. This occurs by random contact with the surface due to Brownian motion, sedimentation, active transport or chemotaxis. Once the microorganisms have been transported to a surface, initial adhesion to the surface occurs, for example, as a result of Lifshitz-van der Waals forces, acid-base interactions and electrostatic forces between negatively charged microorganisms and positively charged domains. The microorganisms then excrete extracellular polymers, which form an extracellular polymeric substance composed of polysacchSlides., nucleic acids, amphiphilic, humic substances and proteins. The extracellular polymeric substance forms a matrix that interconnects and binds together microorganisms attached to the surrounding surface. Thus, the microorganisms are anchored to .the surface, which can be all kinds of materials such as metals, plastics, soil particles, medical implant materials and tissue. Once anchored to a surface, biofilm microorganisms carry out a variety of detrimental or beneficial reactions (by human standards), depending on the surrounding environmental conditions. It is, therefore, desirable to remove and/or destroy the biofilm microorganisms on the surface. The pasteurisation process has been used for a number of years to destroy microorganisms. In this process, the microorganisms are subjected to high temperature and, optionally, high pressure. Microorganisms can also be removed from surfaces by simple washing and sanitisation of the. surface with fresh water or with soap or simple detergents. Washing removes the majority of the microorganisms but does not prevent the growth of any microorganisms that remain. Microorganisms can also be destroyed by contacting them with antimicrobial agents, which are poisonous to microorganisms, A large number of anti-microbial agents are known. For example, bacteriocidal, fungicidal, algicida], yeasticidal and moldicidal agents are known. The anti-micxobial agents can destroy microorganisms that are present in a wide range of environments such as medical, industrial, commercial, domestic and marine environments. Many of the known anti-microbial agents have previously been included in compositions for use in various applications and environments. For example., EP-A-0233954 describes a composition for treating a solid material to give it anti-mi crobial, hydrophilic and anti-static properties. The composition comprises a quaternary ammonium salt-containing silane, an organopolysiloxane and, optionally, an organic solvent. EP-A-0206028 describes a method of promoting the growth of plants. The method comprises applying a specific quaternary ammonium compound, which may be formulated as an aqueous solution. EP-A-0181182 describes an emulsion that comprises water, a water immiscible liquid, a cationic silane and, optionally, a co-surfactant. WO-A-93/10209 describes a composition for sterilising, disinfecting, cleaning and lubricating medical and dental devices. The composition comprises a water-soluble or water-dispersible disinfecting and/or sterilising agent, a surfactant and a water-soluble polymer having lubricating characteristics. WO-A-92/21320 describes medicated shampoo compositions that include anti-microbial agents. The compositions include an anti-microbial agent comprising a fatty acid monoester of a polyhydroxyl alcohol, a chelating agent and a cleansing agent. US-A-5244666 describes a liquid preparation for use as a presurgical skin scrub or wound disinfectant. The preparation comprises a quaternary ammonium compound, a substituted phenolic compound, -water and sodium lauryl sulfate. JP-9175904 describes an agricultural composition that comprises 1,2- benzisothiazoline-3-on, dimethyl polysiloxane, water and N-t-butyl-N'-(4- ethy]benzoyl)-3,5-dimethylbenzohydrazide. The known anti-microbial agents and the compositions that contain these anti-microbial agents destroy microorganisms by a number of different mechanisms. Chlorinated compounds, such as hypochlorites (bleaches) can act as antimicrobial agents. Traditional bleach includes sodium hypochlorite. Sodium hypochlorite breaks down to provide chloride and chlorate. Chlorate is highly toxic to life forms. Although bleaches are useful for destroying a wide range of microorganisms, typically they only work for a short term. This is because their efficacy decreases rapidly once they have broken down. Thus, bleaches do not provide long-term passive anti-microbial control and sanitisation. By "passive control" we mean that the substrate counters microbial infection on its own by some property within it, so that it does not require a cleaning regime to be effective at controlling microorganisms. Furthermore, bleaches can decompose to produce chlorine gas, which is known to be harmful to the environment. Thus, the use of chlorinecontaining compounds is to be avoided where possible. Other known anti-microbial agents include phenol and compounds thereof, arsenene and salts of arsenic. Examples of useful phenol compounds include polychlorinated biphenols, such as triclosan. Other known antimicrobial agents that are commonly used include .organic and inorganic salts of heavy metals such as silver, copper or tin. For example, colloidal silver can be used. Phenol compounds typically are highly toxic to humans and animals as well as to microorganisms. Consequently the anti-microbial agents are dangerous to handle, and specialist handling, treatment and equipment are therefore required in order to -handle these anti-rnicrobial agents safely. Anti-microbial agents can also be difficult to handle if they are strongly acidic or alkaline. The manufacture and disposaj of compositions comprising this type of anti-microbial agent can, therefore, be problematic. There can also be problems associated with the use of compositions containing highly toxic anti-microbial agents, particularly in consumer materials where it is difficult to ensure that they are used for designated purposes. Herein, unless the context indicates otherwise, "toxicity" is intended to refer to toxicity to complex organisms such as mamroals. References to "toxic" are to be construed accordingly. Anti-microbial agents based on phenols and heavy metals typically are only effective against certain microorganisms, such as fungi. Their use is, therefore, limited because they are not effective against all types of microorganism. Additionally, some anti-microbial agents, such as biphenol, do not remain active for extended periods because they are volatile and do not remain on the surface to which they are applied. Once the anti-microbia] agents and/or their breakdown products enter the environment then they can affect the health of life forms that they were not intended to affect. Moreover, the anti-microbial agents and their breakdown products are often highly stable and can cause environmental problems for long periods of time. For example, the metal salts produce toxic rinsates., which are poisonous to aquatic life. Once the toxic compounds enter the environment they are not easily broken down and can cause persistent problems or •unknown consequences. For example, colloidal silver, tributyl tin and diuron can remain in the environment for extended periods of time. The combustion of poly chlorinated biph en ol compounds produces dioxins, which are harmful to the environment. Other anti-microbial agents currently in use include antibiotic type compounds, such as penicillin. Antibiotics disrupt the biochemistry within microorganisms, for example by selectively diluting solutions to destroy or inhibit the growth of harmful microorganisms. Although antibiotics are effective, it is currently believed that they may selectively permit the development of resistant strains of the species that they are used against. These resistant strains are then able to reproduce unimpeded by the use of known antibiotics. Thus, there is a growing concern that wide and uncontrolled use of antibiotic materials in the wider environment, as opposed to their controlled use in medical contexts, could produce significant long-term risks. Antibiotics are, therefore, considered inappropriate for general use in a non-medica) environment. There is also a risk that resistant strains can occur with other types of antimicrobial agent, which can have a biochemical effect. For example, triclosan resistance is discussed in Chuanchuen et al., "Multidrug Efflux Pumps and Triclosan Resistance in Pseudomonas Aeruginosa", 100th General Meeting of the American Society for Microbiology, May 21-25, LA; Meade et al., "Unique Mechanism of Triclosan Resistance Identified in Environmental Isolates", 100* General Meeting of the American Society for Microbiology., May 21-25, LA; Suzangar et al.3 "An Evaluation of Biocidecontaining Materials for their Surface Colonisation-resistance and Other Properties", 100* General Meeting of the American Society for Microbiology, May 21-25, LA. Thus, there is a need for an anti-microbial composition that is effective against a wide variety of microorganisms for long periods of time and which can be used safely and conveniently. According to an aspect of the invention there is provided an anti-microbia] composition comprising (i) a first compound having a high surface tension of from 20 to 35 mN/m, (ii) a second compound having a low surface tension of from 8 to 14 mN/m, (iii) a first anti-microbial agent and (iv) a polar solvent, wherein the composition acts substantially to prevent the formation of microbial colonies on or at a surface of the composition. The anti-microbial composition of the invention is highly effective and works with a broad range of microorganisms. It seems that the anti-microbial composition of the invention works by providing a surface to which microorganisms are substantially prevented from adhering and attaching. In other words, the composition of the invention substantially prevents the occurrence of stage (i) of the biofilrn formation process. This means that the microorganisms cannot then multiply and form biofilms. It is thought that the surface provided by the anti-microbial composition prevents adhesion and attachment of microorganisms due to the interaction of two compounds of high and low surface tension, which have opposing surface tension effects. 7 The prevention of the formation of a biofilm and the greatly reduced and attenuated colonies of microorganisms provides a substantially reduced risk due to infection or contamination. This has the beneficial effect of sanitizing products that incorporate the anti-microbial composition. The anri-rnicrobial composition of the invention typically is also able to break down biofilms that have already formed. It seems that the composition of the invention achieves this by dispersing the biofilms and effectively spreading out the cell walls so as to cause them to break down. The composition may also cause thinning and distortion of the biofflm, which makes the biofilm more susceptible to the anti-microbial agents and, therefore, increases the effectiveness of the anti-microbial agents in the composition. As the anti-microbial composition of the invention physically disrupts the adhesion and attachment of a microorganism to a surface, which is a feature that is common to a wide range of microorganisms, including bacteria, fungi and moulds, the composition is effective against a broad range of microorganisms- Thus, an advantage of the anti-microbial composition of the invention is that it is able to prevent a broad range of microorganisms from adhering and attaching to the surface and, therefore, from forming a biofilm. Large numerous colonies are also substantially prevented from forming. Thus, the ability of the colony to grow is substantially reduced or even prevented. The anti-microbial composition of the invention is, therefore, general in its control of microorganisms. It seems that as well as preventing the growth of colonies, the anti-microbial composition of the invention increases the relative age of the colony because new microorganisms are prevented from being produced. Thus, the anti-microbial agents of the composition are brought into contact with "older" microorganisms that are more susceptible to anti-rnicrobial agents than, newer ones. The anti-microbial agents are, therefore, more effective at lower concentrations than those that are normally used. Thus, the composition of the invention increases the efficacy of, the anti-microbial action of the anti-microbial agents compared to when they are used alone. The anti-microbial composition of the invention can easily be incorporated into other materials., such as functional materials. "When incorporated into such materials, these become anti-microbial in nature and the surface of the formulation will be modified so as to substantially prevent the microorganisms from adhering and attaching thereto. Another advantage of the anti-microbial composition is that it need not comprise combinations of materials that are highly toxic to mammals. The anti-microbial agents used in the anti-microbial compositions are typically well known and widely understood and tested anti-microbial agents. The efficacy of the known anti-microbial agents is amplified in the compositions of the invention. Therefore, anti-microbial agents that have a low toxicity can be used in the anti-microbia] compositions. In contrast, new antimicrobial agents for known techniques of sanitization use '"stronger", more toxic and/or little tested materials. The anti-microbial composition of the invention also does not comprise materials that produce highly persistent residues or rinsates or products that contain heavy metals and their salts. Thus, there is a greatly reduced risk of long term hazards associated with the anti-microbial compositions. The composition of the invention does not interfere with the biochemical reproductive pathways of the microorganisms it controls. The risk of resistance build up and the development of resistant strains is, therefore, highly unlikely. The surface tension of the first compound is greater than that of the second compound and is preferably less than the surface tension of water at any specified temperature. Thus, the first compound can typically act to reduce the surface tension of water. The surface tension of the first compound is from 20 to 35 mN/m at 20°C. The surface tension of the second compound is from 8 to 14 mN/m at 20°C, more preferably 10 mN/m at 20°C. The low surface tension of the second compound reduces non-specific bonding with other components of the composition, particularly bonding with aqueous or hydrated materials. The first compound is preferably hydrophobia The second compound is preferably hydrophilic. This appears to provide a composition that is typically stable in both hydrophobic and hydropbjlic materials. Additionally, the hydrophobic first compound typically attracts the hydrophilic second compound, so as to provide the desired opposing surface tension effects. This combination of properties is thought to create a microscopic turbulent effect that is disruptive to the formation of a biofilm. The fact that this effect is microscopic means that it has a great efficacy on microorganisms but not on larger macroorganisms. Whilst it is preferred that the first compound is hydrophobic and the second compound is hydrophilic, it is possible for the first compound to be hydrophilic and the second compound to be hydrophobic. Preferably, the first compound is a second arrti-microbial agent. Thus, as well as contributing to the surface effects, the first compound also acts as an anti-microbial agent. However, the efficacy of the second anri-microbial 10 agent is improved by the inclusion of the other components of the composition. By the term "anti-microbial agent" we mean any chemical substance that can destroy microorganisms. The first and second anti-microbial agents (hereinafter referred to generally as the anti-microbial agents) present in the compositions of the invention are typically well known and have been subject to research by the regulatory authorities. The anti-microbial agents generally have some effect when they are used alone. However, the efficacy of the anti-microbial agents is amplified when they are used in combination with the other components of the compositions of the invention. Preferably, the composition of the invention comprises two or more antimicrobial agents. A typical composition insy comprise four anti-microbial agents. The anti-microbial agents are preferably of a polar nature. This-enables them to associate with the other components of the composition, for example by hydrogen bonding or non-chemical bonding. This association brings the anti-microbial agents into direct association with the microorganisms as the other components of the composition of the invention themselves associate with the microbial wall. Thus, the antimicrobial agents are effective at low concentrations. The anti-microbia] agents are not thought to form a chemical bond with the first and second compounds. Preferably, the composition comprises at least one anti-microbial agent selected from bacteriocidal,, fbngicidal, algicidal, yeasticidal and moldicidal 11 agents. More preferably, the composition comprises bacteriocidal, ftragicidal andmoldicidal agents. The first anti-microbial agent is preferably an amphoteric compound, an iodophore, a phenolic compound, a quaternary ammonium compound, a hypochlorite or a nitrogen based heterocyclic compound. The second anti-mi crobial agent is preferably a surfactant, more preferably a quaternary ammonium compound. Both the first and second antimicrobial agents may each comprise a quaternary ammonium compound. Preferably, the anti-mi crobial compositions of the invention comprise one or more quaternary ammonium compounds, phenolic compounds and nitrogen based heterocyclic compounds as the anti-mi crobial agent. Quaternary ammonium compounds that are suitable for use in the invention include compounds of formula R'R2RJRTOr., in which one or two of the R groups are alkyl, optionally substituted by aryl or optionally interrupted by aryl or a heteroatom, such as oxygen, and the other R groups are the same or different and are Cj to G Preferred quaternary ammonium compounds include benzalkonium halides, aryl ring substituted benzalkonium halides, such as ethyl-substituted benzallconium halides, and twin chain quaternary ammonium compounds, such as di alky 1 dim ethyl ammonium compounds wherein the two nonmethyl alkyl groups are selected from medium and long chain alkyl groups, such as C8 to Cj2 alkyl, preferably octyl and dodecyl. Suitable quaternary ammonium compounds in which an R group (i.e. R1, R , R , R ) contains a heteroatom include domiphen bromide, benzalkonium 12 chloride and methylbenzalTconrum chloride. Other quaternary ammonium compounds suitable for use in the antimicrobial composition include allcylpyridinium compounds, such as cetylpyridinium chloride, and bridged cyclic amino compounds such as the hexaminium compounds. Particularly preferred quaternary ammonium compounds include benzenethanaminiumn N-dodecyl-N,N-dimethylchloride, benzenethanarninmmn N-dodecyl-N^-dimetbyl-N-tetradecylchloride and berizyl-Cn-C^-alkyldimethyl-anirnoniuinchloride. Arnphoteric compounds suitable for use in the present invention include long chain N-alkyl derivatives of amino acids. Long chain N-alkyl derivatives of glycine, alanine and beta-amino butyric acid are preferred. Particularly preferred compounds include dodecyl beta-alanine, dodecyl beta-armnobutyric acid, dodecyiamino-di(aminoethylamino)glycine and N- (3-dodecylamino)propy]glycine. By the term "iodophores" we mean complexes of iodine or triodine -with a carrier, such as a neutral polymer. The carrier typically increases the solubility of iodine in water, provides a sustained release of the iodine and reduces the equilibrium concentrations of free iodine. Suitable polymeric carriers from which iodophores can be prepared include polyvinylpyrrolidone, polyether glycols such as polyethylene glycols, polyvinyl alcohols, polyacrylates, polyamides, polyalkylenes and polysaccharides. Suitable phenolic compounds include methyl, ethyl, butyl, halo and aryl 13 substituted phenol. Preferred phenolic compounds include 2-phenylphenol, 2-benzyl-4-chloropheno], 2-cyclopentanol-4~cb]orophenol, 4-t-amylpheno], 4-t-butylphenol, 4-cWoro-2-penrylphenol, 6-ch]oro-2-pentylphenol,, pchloro- meta-xylenol, 2:>4,4-trichloro-2-hydroxydiphenol, thymol, 2-ipropyl- 3-methylphenol, chlorothymol, 3-methyl-4-ch.loroph.enol, 2,6- dichloro-4-n-alkyl phenols, 2,4-dichJoro-meta-xylenol, 2,4,6- trichlorophenol and 2-benzyl-4-chlorophenol. Suitable hypochlorites include alkali metal and alkaline earth metal hypochlorites, such as the hypochlorites of lithium, sodium, potassium and calcium. Other snitable hypochlorites include chlorinated trisodium phosphate and their various hydrates. Other suitable chlorine containing or chlorine releasing agents include chlorine dioxide and its precursors, as well as NrN-dichloro-4-carboxybenzenesulponamide (halazone), 1,3-dichloro- 5,5-dimeihylhydantoin (halane) and various chloroisocyanuric acid • derivatives. Suitable nitrogen based heterocyclic compounds include pyridine derivatives, such as 4-pyridine carboxylic acid hydrazide, sodium 2- pyri din ethiol-1-oxide and bis-(2-pyridylthio)ziTJc-l,l-dioxide, triazoles, thiazoles and imidazoles. A particularly preferred anti-microbial composition comprises benzenethanaminiumn N-dodecyl-N,N-dimethylchloride, benzenethanaminiumn N-dodecyl-N,N-dimethyl-N-tetradecylchloride, benzyl-Cn-C^-alkyldimemyl-ammoniumchloride, 2-phenylphenol, 2-octyl- 2H-isothiazol-3-one, 5-chloro-2-methyl-2H-isothiazol-3-one and 2-methyl- 2H-isothiazol-3-one. The particular anti-microbial agents selected for use in the composition will 14 vary depending upon the environment in which the composition is intended to be used. The second compound is preferably chemically inert and has a structure that attaches to virtually any substrate. The second compound can, therefore, remain at a surface for long periods of time. This means that the composition of the invention can easily be recharged. The second compound is also capable of associating with the other components of the composition of the invention by means of non-chemical bonds and typically can adhere to and attract a wide range of polar materials including various anti-microbial agents. The second compound is preferably a surfactant or oil, more'preferably a short chain surfactant or oil. By the term "short chain" ~we mean Cn to Czo- Suitable second compounds include silanes, polyethylene glycol, sodium lauryl sulphate, soya lecathin and preferably siloxanes such a^ polysiloxanes or silicones. A preferred second compound is polydimethylsiloxane and a particularly preferred second compound is polydimethylhydroxysiloxane. For example, a polydimethylhydroxysiloxane having a viscosity of from 100 to 400 centistokes may be included in the compositions of the invention. Preferably, the composition comprises from 1 to 4 % by volume of the second compound; however other proportions are possible and lie within the scope of the invention. Suitable polar solvents for use in the composition include water, alcohols, esters, hydroxy and glycol esters,, polyols and ketones. It seems that the 15 polar solvent helps to provide a composition that is stable and does not separate out into its various components. Preferred alcohols for use in the composition include straight or branched chain C] to C5 alcohols, particularly methanol, ethanol, propanol, isopropanol, n-butanol, sec-butanol, tert-butanol, isobutanol, 2-methyl-lbutanol, 1-pentanol and amyl alcohol (mixture of isomers). Preferred esters for use in the composition include methyl acetate, ethyl acetate., n-propyl acetate, iso-propy] acetate, n-butyl acetate, iso-butyl acetate, sec-butyl acetate, amyl acetate (mixture of isomers), methylamy] acetate, 2-ethylhexyl acetate and iso-butyl isobutyrate. Preferred hydroxy and glycol esters for use in the composition include methyl glycol acetate, ethyl glycol acetate, buty] glyco) acetate, ethyl diglycol acetate, butyl diglycol acetate, ethyl lactate, n-butyl lactete, 3- methoxy-n-butyl acetate, ethylene glycol diacetate, polysolvan 0, 2- methylpropanoic add-2,2,4-trimethyl-3-hydToxypentyI ester, methyl glycol, ethyl glycol, isopropyl glycol, 3-methoxybutanol, butyl glycol, iso-butyl glycol, methyl diglycol, ethyl diglycol, butyl diglycol, isobutyl diglycol, diethylene glycol, dipropylene glycol, ethylene glycol monohexyl ether and diethylene glycol monohexyl ether. Preferred polyols for use in the composition include ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, hexylene glycol, diethylene glycol, triethylene glycol and dipropylene glycol. Preferred ketones for use in the composition include isobutyl hepty] ketone, cyclohexanone, methyl cyclohexanone, methyl isobutenyl ketone, pentoxone, acetyl acetone, diacetone alcohol, isophorone, methyl butyl ketone, 16 ethyl propyl ketone, methyl iso'butyl ketone, inethyl amyl ketone, methyl isoamyl ketone, ethyl butyl ketone, ethyl amyl ketone, methyl hexyl ketone, diisopropyl ketone, diisobutyl ketone, acetone, methyl ethyl ketone, methyl propyl ketone and diethyl ketone, Particularly preferred polar solvents for use in the composition include isopropanol, diethylene glycol and dipropylene glycol. Preferably, the composition comprises from 1 to 70 % by volume of the polar solvent, but since the primary purpose of the solvent is dilution virtually any proportion of polar solvent is believed to be possible within the scope of the invention. An especially preferred anti-microbial composition comprises 32 % by volume of a mixture of benzeuethanaroim'umn N-dodecyl-N,Ndimethylcriloride and benzenelhanaminiumn N-dodecyl-N,N-dirnethyl-Ntetradecylchloride (2.33:1), 6.0 % by volume of a mixture of benzyl-C]2- C)6-a!] volume of 2-octyl-2H-isothiazol-3-one, 16.0 % by volume of a mixture of 5-chloro-2-methyl-2H-iso1hiazol-3-one and 2-methyl-2H-isothiazo]-3-one (3:1), 1.0 % by volume of a blend of polysiloxanes and balance by volume isopropanol. Another especially preferred anti-microbial composition comprises 32 % by volume of a mixture of benzenethanaminiumn N-dodecyI-N,Ndimethyl chloride and benzenethanaminiumn N-dodecyl-N,N-dimethyl-Nterradecylchloride (2.33:1), 6.0 % by volume of a mixture of benzyl-C]2- C]6-alkyldimethyl-ammoniumch]oride and 2-phenylphenol (2:1), 6.0 % by volume of 2-octyl-2H-isothiazol-3-oneJ 16.0 % by volume of a mixture of 5-chJoro-2-methyl-2H-isothiazol-3-one and 2-methyl-2H-isothiazol-3-one 17 (3:1), 1.0 % by volume of polydiraethylhydroxysiloxane and balance by volume isopropanol. Another especially preferred anti-microbial composition comprises 5.0 % by volume of a mixture of benzenefhanaminiurrm N~dodecyl-N,Ndimethylchloride and benzenethanammiurrm N-dodecyl-N7N-dimethyI-Ntetradecylchloride (2.33:1), 5.0 % by volume of a mixture of ben2yl-Ci2- Cjg-allcyldimethyl-ammoniurnchlorideand 2-phenylpbenol (2:1), 12.0 % by volume of 2-octyl-2H-isothiazol-3-one, 32.0 % by volume of a rmxture^of" 5-chJoro-2-methyl-2H-isothiazol-3-one and 2-me1hyl-2H-isothiazoI-3-one (3:1), 1.0 % by volume of a.blend of polysiloxanes and balance by volume diethyleneglycol. A further especially preferred anti-microbial composition comprises 6.0 % by volume of a mixture of benzenethanaminiujixa N-dodecyl-N,Ndimethylchloride and beiizenethanaininiuinn N-dodecyl-N^N-dimethyl-Ntetradecylchloride (2.33:1), 6.0 % by volume of a mixture of benzyl-Cj2- C16-alkyldimethyl-ammoniiimchloride and 2-phenylpbenoI (2:1), 16.0 % by volume of 2-octyI-2H-isothiazol-3-one, 32.0 % by vohjme of a mixture of 5-chloro-2-methyl-2H-isothiazol-3-one and 2-methyl-2H-isothiazol-3-one (3:1), 1.0 % by volume of a blend of poylsiloxanes and balance by volume isopropanol. Yet another especially preferred anti-microbial composition comprises 6.0 % by volume of a mixture of benzenethanarniniumn N-dodecyl-N^Ndimethylchloride and benzenethanaminiumn N-dodecyl-N7N-dimethyl-Nterradecylchloride (2.33:1), 6.0 % by volume of a mixture of benzyl-Cj2- C]6-alkyldimethyl-ammoniumchlorideand 2-phenylphenol (2:1), 16.0 % by volume of 2-octyI-2H-isothiazol-3-one, 32.0 % by volume of a mixture of 5-chloro-2-methyl-2H-isolhiazol-3-one and 2-methyl-2H-isothiazol-3-one 18 (3:1), 1.0 % by volume of a blend of poJysitoxanes and balance by volume dipropyleneglycol. According to a further aspect of the invention, there is provided a formulation comprising an anti-microbial composition and at least one other functional material. Suitable functional materials' include plastics, fibres, coatings, films, laminates, adhesives, sealants, clays, china, ceramics, concrete, sand, paints, varnishes, lacquers, cleaning agents or settable or curable compositions such as fillers, grouts, mastics and putties. The plastics may be in the form of films, sheets, stabs and molded plastic parts. Suitable plastics materials may be prepared from polyesters such as polyethylene tereph thai ate, polybutylene terephthalate, polyamides such as Nylon, polyimides, polypropylene, polyethylene, polybutylenes, polymethylpentene, polysiloxane, polyvinyl alcohol, polyvinylacetate, ethylene-vinylacetatc, polyvinyl chloride, pojyvinylidene cbJoride, epoxy, phenolic and polycarbonate cellulosics, cellulose acetate, polystyrene, polyurethane, acrylics, polymethyl methacrylate, acrylonitrile, butadienestyrene copolymer, acTylonitrilestyrene-acrylic copolymers, acetals, polyketones, polyphenylene ether, polyphenylene sulfide, polyphenylene oxide, polysulfones, liquid crystal polymers and fluoropolymers, amino resins, tfiermo plastics, elastomers, rubbers such as styrene butadiene rubber and acrylonitrile butadiene rubber, polyacetal (polyoxymethylene), and blends and copolymers thereof. Formulations comprising the anti-microbial composition and a plastics material as the functional material may, for example, be used to form products such as automobile parts, shower curtains, mats, protective covers, 19 tape, packaging, gaskets, waste containers, general purpose containers, brush handles, sponges, mops, vacuum cleaner bags, insulators, plastic film, indoor and outdoor furniture, tubing, insulation for wire and cable, plumbing supplies and fixtures, siding for housing, liners, non-woven fabrics, kitchen and bathroom hardware, appliances and equipment, countertops, sinks, flooring, floor covering, tiles, dishes, conveyer belts, footwear including boots, sports equipment and tools. Suitable fibres may be prepared from acetate, polyester such as PET and PTT, polyolefms, polyethylene, polypropylene, polyamides such as Nylon, acrylics, viscose, polyurethane, and Rayon, polyvinyl alcohol, polyvinyl chloride, polyvinylidene chloride, polysaccharide, and copolymers and blends thereof. Formulations comprising the anti-microbial composition and a ffbre as the functional material may., for example, be used in applications such as mattress cover pads and filling, pillow covers, sheets, blankets. fiberSJl for quilts and pillows, curtains, draperies, carpet and carpet underlay, rugs, upholstery, table cloths, napkins, wiping cloths, mops, towels, bags, wall covering fabrics, cushion pads, sleeping bags and brush bristles. The fibres are also suitable for use in automotive and truck upholstery, carpeting, rear decks, trunk liners, convertible tops and interior liners. Furthermore, the fibres are suitable for use in umbrellas, outerwear, uniforms., coats, aprons, sportswear, sleepwear, stockings, socks, hosiery caps, and undergarment and inner liners for jackets, shoes, gloves and helmets, trim for outerwear and undergarments as well as brush bristles, artificial leather, filters, book covers, mops, cloth for sails, ropes, tents, and other outdoor equipment, tarps and awnings. Coatings suitable for use in the formulations include water-borne, solvent- 20 borne, 100% solids and/or radiation cure coatings. The coatings may be liquid or powder coatings. Suitable coatings, films and laminates include alkyds, amino resins, such as melamine formaldehyde and urea formaldehyde, polyesters, such as unsaturated polyester, PET, PBT, polyamides such as Nylon, polyimides, polypropylene, polyvinylacetate, ethylene-vinylacetate, polyvinyl chloride, polyvinylidene chloride, epoxy, phenolic and polycarbonate cellulosics, cellulose acetate, polystyrene, polyirrethane, acrylics, polymethyl methacrylate, acrylonitrile-butadiene-styrene copolymer, acrylonitrilestyreneacrylic copolymers, acetals, polykctones, polyphenylene ether, polyphenylene sulfide, polyphenylene oxide, polysulfones, liquid crystal polymers and fluoropolymers, thermoplastic elastomers, rubbers such as styrene butadiene rubber, acrylonitrile' butadiene rubber, polyacetal (polyoxymethylene), and blends and copolymers thereof. Formulations comprising the anti-microbial composition and coatings as the functional material may, for example, be used on walls, wall boards, floors, concrete, sidings, roofing shingle, industrial equipment, natural and synthetic fibres and fabrics, furniture, automotive and vehicular parts, packaging, paper products (wall coverings, towels, book covers) barrier fabrics, and glazing for cement tile and for vitreous china used in plumbing fixtures such as toilets, sinks, and countertops. Adhesives and sealants suitable for use in the formulations include hot-melt, aqueous, solvent borne, 100% solids and radiation cure adhesives and sealants. Suitable adhesives and sealants include alkyds, amino resins such as melamine formaldehyde and urea formaldehyde, polyesters such as 21 unsaturated polyester, PET, TBT. polyamides such as Nylon, polyimide polypropylene, polyethylene, polybutylene, polymethylpentene., polysiloxane., polyvinyl alcohol, polyvinylacetate, efhylene-vinylacetate, polyvinyl chlorides such as plastisol, polyvinylidene chloride, epoxy, phenol and polycarbonate, cellulosics, cellulose acetate, polystyrene, polyurethane, acrylics., polymethylmethacrylate, acrylonitrilebutadienestyrene copolymer, acrylonitrile-styrene-acrylic copolymers, acetals, polyketones, polyphenylene ether, polyphenylene sulfide, polyphenylene oxide, polysulfones, liquid crystal polymers and fluoropolymers, thermoplastic elastomers, rubbers (including styrene butadiene rubber, acrylonitrile butadiene rubber, CR), polyacetal (polyoxymethylene), and blends and copolymers thereof. Formulations comprising the anti-mi crobial composition and an adhesive or sealant as the functional material may, for example, be used in the manufacture of wood and plastic composites, adhesives for ceramic tiles, v/ood, paper, cardboard, rubber and plastic, glazing for windows, grout, sealants for pipes, adhesives, sealants and insulating materials for appliances, bathrooms, showers, kitchens, and construction. Formulations comprising the anti-microbial composition and clay, china, ceramics, concrete, sand or grout as the functional material may, for example, be used in toilets, sinks, tile, flooring, stucco, plaster, cat litter, drainage and sewerage pipe. The anti-microbial composition can be combined into a very wide variety of functional compounds for the manufacturing, contracting and construction industries. The nature of the anti-microbial composition may be varied according to the particular functional compounds and the number and nature of microorganisms present in the particular functional compound or 22 environment in which it is used. The formulation preferably comprises from 0.1 wt% to 5.0 wt%, more preferably from 0.1 to 4.0 wt%3 even more preferably from 0.5 wt% to 2.0 wt%, of the anti-microbialcomposition. The anti-micTobia] composition is highly effective against a broad range of microorganisms even when it is combined -with another functional material to provide the formulation of the invention. The formulation can, optionally, be applied to a surface. The formulation provides long-term anti-microbia] action, in both dry and damp conditions at the surfaces treated or in which the material is combined. This will lead to a sanin'sation of the surfaces so that the surfaces and products will prevent the rapid replication of microbial species and, thus, substantially reduce the risks of contamination and infection. The anti-mi crobial composition is mobile through most functional materials in which it is incorporated in the formulations of the invention. This is due to the presence of surfactant materials and oils and molecules of short chain length. In order to maintain tin's .mobility, the surfactant materials and oils preferably have a carbon chain length of no greater than 20. The anti-microbia] composition tends to migrate across a concentration gradient and moves to the surface of products into which it has been incorporated. This is similar to the behaviour of plasticiser in polymers. Both the anti-microbial composition and the formulation typically begin to dissociate into their component parts when they have been in continuous contact with water for longer than six to eight hours. The anti-microbia] action, of the anti-microbia] composition and the formulation, is 23 substantially reduced once the composition and formulation have dissociated into their component parts. The components can then act as a carbon source or nutrient for many species of microorganisms. Thus, the anti-microbial composition and the formulation can degrade when submersed in water, to provide a rinsate/leachate of low toxicity and which has a short residence time in the environment. It is thought that the rinsates have a low toxicity because the anti-microbial agents are associated with the second compound and so the composition does not readily dissociate in the presence of water. The formulation can be designed so that it is stable and effective in most manufacturing environments. The formulation is typically stable up to temperatures of 200°C. The property of mobility of the product permits materials that are highly frequently washed or rinsed to be "recharged'1 with the anti-microbial composition during a routine act of cleaning or maintenance. Typically, the anti-microbial composition is incorporated into a simple conventional detergent solution or added to a "final rinse" during cleaning. The anti-microbial composition will be drawn, due to the presence of its hydrophobic elements, into the surface of the product to be "recharged". The sanitizalion properties of the formulation are, therefore, restored without the need for re-manufacture or difficult treatment processes. Any wash off or rinsates containing the anti-microbial composition or formulation diluted by such a re-charging solution and water would quickly dissociate into the biodegradable components as previously discussed. 24 According to a further aspect of the invention., there is provided the use of an anti-microbial composition to prevent the formation of colonies of microorganisms on a surface at which it is provided. According to yet a further aspect of the invention, there is provided the use of a formulation to prevent the formation of colonies of microorganisms on a surface at which it is provided. The anti-microbial composition and formulation have an anti-bacterial effect against a wide range of gram-positive and gram-negative bacteria. For example, they are effective against the following: Bacillus species, such as Bacillus subtilis, Bacillus cereus Brevibacterium species Brucella species, such as Brucella abortus Laclobacillus species Proteus vulgaris Pseudomonas aerueinosa Salmonella species Staphylococcus species, such as Methicillin Resistive Staphylococcus Aureus (MRS A) Streptococcus species Flavobacterium species Escherichia species Aeromonas species "The anti-rnicrobial composition and formulation also have activity against fungi and yeasts, such as: 25 Penicillium species Aspergillus niger Cladosporium species Fusarium species Paecilomyces species Streptomyces species Saccharomyces species, such as S.cerevisiae Monilia albicans The anti-microbial composition and formulation also have activity against certain species of algae such as: Chlorella pyrenoidosa Pleurococcus Anabaena species According to another aspect of the invention, there is provided a method of manufacturing an anti-mi crobiaJ composition, the method comprising the steps of (i) mixing the first compound and the first anti-microbial agent together, (ii) adding the second compound to the mixture of first compound and the first anti-microbial agent, (iii) adding the polar solvent to the mixture of the first and second compounds and the first anti-mi crobial agent and (iv) agitating the resulting mixture until a clear solution is formed. According to yet a further aspect of the invention, there is provided a method of manufacturing a formulation, the method comprising the step of adding the anti-microbial composition to the functional compound. The present invention as now illustrated but not limited with reference to the following examples. 26 Example 1 Preparation of Anti-mi crobial Composition ("D4L") A composition according to the present invention comprising components (a) to (f) in the amounts indicated was prepared: (a) 32.0% by volume of a mixture of two benzalkonjum chlorides (in a ratio of 2.33:1) i.e. benzenethanaminmmn N-dodecyl-N,N~dimethylchloride and benzenethanaminiurrm N-dodecyl-N,N-dimethyl-N-tetradecyl-chloride (Trade Name: BAC-50m); (b) 6.0% by volume of a mixture of arnraonrumchJoride (CAS no. 68424-85-1) and 2-phenyl phenol in the ratio 2:1 (Trade Name: Acticide SOX); (c) 6.0% by volume of 2-octy]-2H-isothiazol-3-one (Trade Name: A-DW); (d) 16.0% by volume of a mixture of 5-chloro-2-methyl-2H-isothiazol-3- one and 2-methyl-2H isothiazol-3-one in tbe ratio 3:1 (Trade Name: A-14); (e) 1.0% by volume of polydimetnylhydroxysiloxane (Trade Name: PD-D); and (f) 39% by volume of an isopropanol blend (isopropanol,, n-propanol and water to azeotropic limit about 1.0 %). Anti-mi crobial agents a, b, c and d were mixed together sequentially at room temperature following the sequence described above. The resulting mixture was then agitated thoroughly and the polysiloxane (e) was added to the mixture. The resulting mixture was agitated and isopropanol (f) was added. The mixture was then agitated until a clear solution was obtained. 27 The clear solution is referred to herein as trD4L". Example 2 Preparation of Anti-Microbial Composition ("LCF") A composition according to the present invention comprising components (a) to (f) in the amounts indicated was prepared: (a) 32.0% by volume of a mixture of two benzalkonium chlorides (in a ratio of 2.33:1) i.e. benzenethanaminiumn N-dodecyl-N,N-dimethylch]oride and benzenethanaminiumn N-dodecy]-N,N-dimethyl-N-tetradecyl-chloride (Trade Name: BAC-50m); (b) 6.0% by volume of a mixture of ammoniumchloride (CAS no. 68424-85-1) and 2-phenyl phenol in the ratio 2: 1 (Trade Name: Acticide SOX); (c) 6.0% by volume of 2-phenyl phenol; (d) 16.0% by volume of a 25% solution of l>2-benziothjazolin~3-one hi isopropanol; (e) 1.0% by volume of polydimethylhydroxysiloxane (Trade Name: PD-D); and (f) 39% by volume of an isopropanol blend (isopropanol, n-propanol and water to azeotropic limit about 1.0 %). Anti-mi crobial agents a, b, c and d were mixed together sequentially at room temperature following the sequence described above. The resulting 28 mixture was then agitated thoroughly and the polydimethylhydroxysiloxane (e) was added to the mixture. The resulting mixture was agitated and isopropano] (f) was added. The mixture was then agitated until a clear solution was obtained. The clear solution is referred to herein as CCLCF". Example 3 Preparation of Detergent Formulation comprising the Antimicrobial Agent Composition of Example 1 (i.e. D4L) An amphotenc non-ionic detergent, such as washing-up liquid, having a pH of from 6 to 8, was diluted in water in a ratio of 1 part detergent to 25 parts of water by volume. To this solution was added between 0.5 and 2.0 % by volume of the anti-microbial agent composition prepared according to Example 1 (i.e. D4L). Example 4 Effectiveness of Anti-mi crobial Agent Formulation against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Method Two samples were tested. These were a detergent formulation prepared according to Example 3 comprising 2% by volume of the anti-microbial agent composition of Example 1, and a neutral detergent. The neutral detergent was used as a standard reference. A bacterial culture (0.1 rnJ) in a nutrient medium was applied to a previously sterilised petri dish over an area 7 x 5 cm. The bacterial culture was then allowed to dry for 30 minutes. 29 The inoculated area was then wiped with a test wipe soaked either in water or the test solution to contact the test fluid with the bacteria. The test solution was applied using either an absorbent cloth or an innoculum loop. The innoculated area was also left untreated to provide an "uncleaned control", in which the infected area was not washed or even wiped with water. The bacteria remaining on the surface of the petri dish were numerated after periods of 15 and.30 seconds. The bacteria remaining on the surface of the petri dish were numerated by wetting a sterile swab in a sterile peptone solution (0.1%) and thoroughly rubbing the swab over the area to be sampled, turning the swab as it was robbed over the appropriate area. The swab was then returned to a sterile tube; Ringers solution (5 ml, 1/4 strength) was added; and the swab left for at least 10 minutes. The swab tubes xvere plated out making serial decimal dilutions, using the Miles and Misra Total Viable Count Technique and incubated inverted at 373C overnight. The number of colony forming units (CFU) (taken to be •viable bacterial individuals) was then counted. Calculation The log reduction in bacterial numbers was calculated compared to the water control and the uncleaned control. The total number of CFUs per ml of neat sample was calculated for each test sample and the controls. The log of the number of CFUs for the water control, or the uncleaned control, was calculated to give value A. This was repeated for the test anti- 30 nricro"bial composition to give value B. A-B - Log Reduction (A - log CFUs water or uncleaned control, B = log CFUS test sample) A log reduction of greater than 4 is considered to be effective. Table 1 - Results Composition Anti-mi crobial composition Anti-mi crobial composition Anti-microbial composition i Organism Escherichia col? Staphyhcoccits aureus Pseudomonas aeniginosac Log reduction after 1 5 sees 1.0 >4.9 1.8 Log reduction after 30 sees >4.0 >4.9 3.3 a Total viable count 6.8 x 10" b Total viable count 5.8 x 1C8 c Total viable count 1.5 x 109 Conclusions A 2% by volume solution of the anri-microbial composition gave a log reduction of 1.0 after 15 seconds and >4.0 after 30 seconds when tested against Escherichia coli. A 2% by volume solution of anti-microbial composition gave a log reduction of >4.9 after 15 seconds when tested agarnst Staphylococcus aureus. A 2% by volume solution of anti-microbial composition gave a log 31 reduction of 1.8 after 15 seconds and 3.3 after 30 seconds when tested against Pseudomonas aeruginosa. Example 5 Resistance of Painted Film Formulations containing an Antimicrobial Composition to Dry Film Fungal and Algal Colonisation The following formulations comprising paint and the anti-mjcrobial agent composition of Example 1 were tested: Composition Number Control 1 7 3 4 5 % by volume of Antimicrobial Composition 0.00% 0.50% 0.75% 1.00% 1 .50% 2.00% Method - Dry Film Fungal Resistance Test (Based on British Standard BS3900 Part G6) Each formulation was painted onto 6 x 9 cm gypsum panels. Two coats of each formulation were painted onto the gypsum panels, allowing 24 hours drying time between each coat. When the panels were dry, they were spray inoculated with a mixed spore suspension prepared from fungi (including yeasts) isolated from or known to grow on painted surfaces. The test panels were suspended in a high humidity cabinet at 24°C for four weeks and the resultant fungal growth assessed visually and microscopically. Fungal 32 growth rating was according to BS3900 Part G6. The micro-organisms used were: Aspergillus versicolor Aureobasidiurn puljulans Cladosporium cladosporioides Penicillrum purpurogenum Phoma vio]aceae Rhodotorula rubra Sporobolornyces roseus Stachybotrys chartarum Ulocladium atrum Method — Dry Film Algal Resistance Test - Vermiculite Bed Method Each formulation was painted onto 10 x 10 cm calcium silicate panels. Two coats of each formulation were painted onto the panels, allowing 24 hours drying time between each coat. When the panels were dry, they were weathered using a QUV Accelerated Weathering Tester for 125 hours using a water spray cycle. Each panel was then cut in half. The half panels were placed in the surface of vermjculite (200 g) moistened with water (800 cm3) in a transparent plastic box with a close fitting lid. The panels were each spray inoculated with a mixed algal suspension three times at intervals of two weeks and sprayed with water each week. The panels were incubated for 13 weeks at 20°C -and illuminated with 30 W daylight type fluorescent tubes (giving approximately 1000 lux) for 16 hours per day. The resultant algal growth was assessed visually and microscopically. The microorganisms used were: Chlorella emersonii Gloeocapsa alpicola Nostoc commune 33 Pleurococcus sp. Stichococcus bacillaris Stigeoclonium tenue Trentepohlia aurea Trentepohlia odorata Results Table 2 - Diy Film Fungal Resistance Test Composition Number Control 1 2 oJ A — r ^ % Anti-micTobial agent (by volume) 0.00% 0.50% 0,75% 1.00% 1.50% 2.00% Observed Rating* (4 weeks) 4 (40+) 3(30+) 2(10+) 2(5+) 2(5+) 0(0) ' of replicate panels given. Table 3 - Dry Film Algal Resistance Test Composition Number Control 1 2 3 4 5 % Anti-mi crobial agent (by volume) 0.00% 0.50% 0.75% 1.00% ].50% 2.00% Film Algal Growth — Replicate 1 4 (50+) 3(20+) 2(10+) 2(10+) 2(10+) 2(5+) Rating/Intensity — Replicate 2 4 (40+) 3(20+) 3 (15+) 2(10+) 2(10+) 2(5+) 34 Growth Ratings The first figure represents the fungal growth cover as follows: 0 = No growth 1 = Trace growth 2 = 1 to 10% Coverage of growth 3= 11 to 30% Coverage of growth 4 = 31 to 70% Coverage of growth 5 = 71 to 100% Coverage of growth The second figure in brackets represents the % cover and an assessment of the intensity rating, as follows: 0 = Growth barely visible to the naked eye + = Light growth -H- = Moderate groTvth = Dense rowth Conclusion The control sample (containing no anti-mi crobial composition) was found to be susceptible to dry film fungal and algal colonisation. An addition of 1.0% of the • anti-microbial composition was found to control the fungal and algal population to a level that meets the pass criterion, of below 20%. 35 Example 6 Microbiological Testing against MRSA of Coil Coating Panels Treated with the Anti-microbial Composition of Example 1 Coil coating panels, manufactured by Becker Industrial Coatings Ltd., Liverpool, were treated with a range of concentrations of an anti-microbial composition according to Example 1. The panels were then tested to demonstrate whether they have antibacterial properties against Methicillin Resistant Staphylococcus Aureus (MRSA). The panels were coated as follows: 51 Coil coating panel, 1.0% by volume anti-microbial composition 52 Coil coating panel, 1.5% by volume anti-microbial composition 53 Coil coating panel, 2.0% by volume anti-microbial composition 54 Coil coating panel, 2.5% by volume anti-microbial composition 55 Coil coating panel, 3.0% by volume anti-microbial composition 56 Coil coating panel. 0% anti-microbial composition (control) Method The MRSA culture was diluted to approximately 1.5 x 10" CPU/ml with sterile deionised water. ] ml of this solution was placed on a coil coating panel and was continuously applied over an area of approximately 5 cm x 5 cm using a hand held spreader for a contact period of 1 minute. The culture was immediately recovered from the panel using a swab and was transferred to a universal bottle containing neutralizer (1 ml) and maximum recovery diluent (9 ml). 10 fold serial dilutions were prepared and 0.1 ml aliquots of the dilutions were plated onto nutrient agar, in duplicate. The plates were incubated at 37°C for 24 hours and 48 hours and read using conventional 36 techniques. Tie procedure was repeated with a culture contact time of 5 minutes. All six samples were subjected to the same test protocol. Table 4 - Results Sample SI S2 S3 S4 S5 S6 Contact time 1 min (CFU/ml) 82 73 60 55 49 UxlOJ Contact time 5 min (CFU/ml) 55 50 38 35 30 2.5x10" Conclusion All of the test saropies (SI to S5) produced very significant decreases in the bacterial count (from 1.5 x ICr CFU/roJ) in 1 minute of contact time and further small decreases after 5 minutes. Total bacterial kill was not achieved in 5 minutes of contact. The control sample (S6) produced a small decrease in the bacterial count (from 1.5 x 103 CFU/mJ) in 1 minute of contact time, which may be primarily due to the difficulty of recovering the culture from the panels using swabbing techniques. After 5 minutes of contact time the control samples bacterial count had significantly decreased primarily due to the drying out of the culture during the continuous spreading action on the panels. 37 The coil coatings treated with the anti-microbial composition are effective, at all of the tested concentrations, in very significantly reducing the level of MRSA bacteria when in contact in an aqueous medium for short periods. These coatings would be very effective in assisting in the control of MRSA "bacterial contamination in hospitals and similar environment. Example 7 Microbiological Testing against MRSA of HMG's Panels of Food Safe PVC 94 Laminate Treated with the Anti-microbial Composition of Example 1 Panels, from H. Marcel Guest Ltd (HMG), coated with food safe PVC 94 laminate were treated with a formulation comprising a paint and 2% by volume of the anli-microbial composition prepared according to Example 1 in order to demonstrate whether they have antibacterial properties against Methicillin Resistant Staphylococcus Aureus (MRSA). Samples 51 PVC 94 laminated panel, 2% by volume anti-microbiaj composition, Clear. 52 Control, Clear. 53 PVC 94 laminated panel, 2% by volume antj-microbial composition, White. 54 Control, White. Method The MRSA culture was diluted to approximately 1.5 x 104 CPU/ml with 38 sterile deionised water and 1 ml was placed on a panel and continuously applied over an area of approximately 5 cm x 5 cm using a hand held spreader for a contact period of 1 minute. The culture was immediately recovered from the panel using a swab and was transferred to a universal bottle containing neutralizer (1 ml) and maximum recovery diluent (9 ml). 10 fold serial dilutions were prepared and 0.1 ml aliquots of the dilutions were plated onto nutrient agar, in duplicate. The plates were incubated at 37°C for 24 hours and 48 hours and read using conventional techniques, The procedure was then repeated with a culture contact time of 5 minutes. AJ1 four samples were subjected to the same test protocol. Table 5-Results Sample SI S2 S3 S4 Contact Time 1 min (CPU/ml) 52 2.1 x 1(P 97 4.1 x 10= Contact Time 5 rain (CFU/ml) 30 1.6X102 51 1.9x10* Discussion The test samples (SI and S3) produced very significant decreases in the bacterial count (from 1.5 x 103 CFU/ml) in 1 minute of contact time and further small decreases after 5 minutes. Total bacterial kill was not achieved in 5 minutes of contact. The control samples (S2 and S4) produced significant but smaller decreases in the bacterial count (from 1.5 x 103 CFU/ml) in 1 minute and 5 minutes of contact time. This may be partially due to the difficulty of recovering the culture from the panels using swabbing techniques and to the drying out of 39 the culture during the continuous spreading action on the panels. Conclusions The PVC 94 laminated panels treated with 2% by volume anti-microhial composition, are effective in reducing the level of MRSA bacteria when in contact in an aqueous medium for short periods. These coatings would be likely to be very effective in assisting in the control of MRSA bacterial contamination in hospitals and similar environment. Example 8 Determination of the Anti-microbia] Effect of Coated Test Panels Containing the Anti-microbia] Composition according to Example 1 The microorganisms tested were: Bacillus subtilis NCTC 44878 3.2 x 106 CPU/ml Pseudomonas aeruginosa NCTC 10662 3.6 x 106 CPU/ml Method Test panels were coated with paint/powder coatings containing the antimicrobial composition according to Example 1. The coated test panels were challenged with broth cultures of the two organisms at the above concentrations for 10 minutes contact time. The bacterial suspension was pipetted onto the coated test panel and 40 removed with a swab after 10 minutes. The swab was transferred to maximum recovery diluent and plated onto Standard Plate Count Agar, incubated at 30°C for 24 hours and the total number of colonies counted. Results Table 6 - Paint Coating Panel Number 1 2 . 3 4 5 6 Bacillus subtilis (CFU/ml) 20 7 TNC 60 83 TNC Pseudomonas aeruginosa (CFU/ml) 32 3 TNC 15 41 TNC Table 7 - Epoxy Polygloss Powder Coating Panel Number 1 2 3 4 5 6 Bacillus subtilis (CFU/ml) TNC TNC TNC 30 150 42 Pseudomonas aeruginosa (CFU/ml) TNC 286 132 9 24 30 41 Table 8 - Grey Epoxy Polyester Gloss Powder Coating Panel Number 1 2 3 4 5 Bacillus subtilis (CPU/ml) 4 10 6 TNC TNC Pseud omonas aeruginosa (CFU/ml) 13 9 5 TNC TNC TNC = Too numerous to count Conclusion The results show that the bacteria are almost completely eradicated within 10 minutes contact time by the anti-microbial composition according to Example 1 in many of the paint/powder coating formulations, even though the surface is dry. .A powder coating containing the anti-microbial composition accordin g to Example 1 at the concentrations shown to be effective is, therefore, likely to be highly effective in reducing the number of bacteria on a surface in a short timescale. Example 9 Effectiveness of the Anti-microbial Composition "LCF' of Example 2 The samples tested were as follows: 1000 LCF: 1 % by volume LCF in water 2000 LCF: 2% by volume LCF in water 42 3000 LCF: 3% by volume LCF in water The microorganisms used were: Legionella pneumophila NCTC 11192 Escherichia coli NCTC9001 Staphylococcus aureusNCIMB 12702 Salmonella enteritidis NCTC5188 ListeriamonocytogenesType 1 NCTC7973 Pseudomonas aeruginosa NCIMB 12469 Method The European Suspension Test (Pr En 1276 November 1995) was conducted under the following experimental conditions: Test concentrations: Neat Test temperature: 10°C (+/- 1 °Q Test conditions: Clean (0.3g/2 00ml bovine albumin) Dirty (3g/100ml bovine albumin) Neutraliser: Lecithin 3g/l, polysorbate 80 30g/l, sodium thiosulphate 5g/l, L. histidine lg/1, saponin 30g/l in diluent Contact time: 5 min Temp of incubation: 37°C (+/- 1 °C) Results For the test results to be valid the neutralise! used must be shown to be nontoxic to the bacteria and to adequately neutraJise the product under test. The experimental test conditions must also be validated. 43 To pass the test the product when diluted in Bard water must demonstrate at least a 10^ reduction in viable count when tested under simulated clean or dirty conditions and under the required test conditions. Table 9 - Results for 1000 LCF Test Organisms L. pneumophila E. coli S. aureus S. enteritidis L. monocytogenes P. aeruginosa Clean Conditions Log Reduction 4.12 4.26 4.08 4.44 4.54 4.02 Pass/Fail FAIL FAIL FAIL FAIL FAIL FAIL Dirty Conditions Log Reduction "3.94 4.02 4.10 4.08 4.20 3.90 Pass/Fai FAIL FAIL FAIL FAIL FAIL FAIL Table 10 - Results for 2000 LCF Test Organisms L. pneumophila E. coli S. aureus S. enteritidis L. monocytogenes P. aeruginosa Clean Conditions Log Reduction 4.68 4.76 4.68 4.72 4.86 4.64 Pass/Faij FAIL FAIL FAIL FAIL FAILFAIL Dirty Conditions Log Reduction 4.62 4.34 4.22 4.28 430 4.10 Pass.-7ai FAIL FAIL FAIL FAIL FAIL FAIL 44 Table 1 1 - Results for 3000 LCF Test Organisms L. pneumophila E. coli S. aureus S- enteritidis L. monocytogenes P. aeruginosa Clean Conditions Log Reduction 4.84 4.72 4.84 4.92 4.9S 4.79 Pass/Fail FAIL FAIL FAIL FAIL FAIL FAIL Dirty Conditions Log Reduction 4.68 4.27 4,44 4.95 4.54 4.62 Pass/Fai FAIL FAIL FAIL FAIL FAIL FAIL Conclusion All three samples, 1000 LCF, 2000 LCF and 3000 LCF, failed the European Suspension Test at 10°C for all of the microorganisms used. However, although the samples failed this stringent test, they did display significant anti nricrobial activity against all of the organisms. Example 10 Effectiveness of the Anti-microbial Composition irD4U* of Example 1 The samples tested were as follows: 500 D4L: 0.5% by volume D4L in water 1000 D4L: 1.0% by volume D4L in water 1500 D4L: 1.5% by volume D4L in water 2000 D4L: 2.0% by volume D4L in water The microorganisms used were; Legionella pneumophila NCTC 11192 45 Escherichia coli NCTC9001 Staphylococcus aureus NCIMB 12702 Salmonella enteritidis NCTC5188 Listeria monocytogenes Type 1 NCTC7973 Pseudomonas aerugmosa NCIMB 12469 Method The European Suspension Test was conducted under the following experimental conditions: Test concentrations: Neat Test temperature: 10°C (+/- 1 °Q Test conditions: Clean (0.3g/l 00ml bovine albumin) Dirty (3g/100ml bovine albumin) Neutralise!-: Lecithin 3g/l, polysorbate 80 SOg/1, sodium thiosujphate 5g/l, L. histidine Is/I, saponin 30g/l in diluent Contact time: 5 min Temp of incubation: 37°C (+/- 1 °C) Results For the test results to be valid the neutraliserused must be shown to be nontoxic to the bacteria and to adequately neutralise the product under test. The experimental test conditions must also be validated. To pass the test the product when diluted in hard water must demonstrate at least a 105 reduction in viable count when tested under simulated clean or dirty conditions and under the required test conditions. 46 Table 12 - Results for 500 D4L Test Organisms L. pneurnophila E, coli S, atireus S. enteritidis L. monocytogenes P. aeruginosa Clean Conditions Log Reduction 4.64 4.76 4.80 4.82 4.89 4.50 Pass/Fail FAIL FALL FAIL FAIL FAIL FAIL Dirty Conditions Log Reduction 4.48 4.55 4.70 4.75 4.72 4.36 Pass/Fai FAIL FAIL FAIL FAIL FAIL FAIL Table 13 - Results for 1000 D4L Test Organisms L. pneumophjla E. coli S. aureus S. enteiitidis L. monocytogenes P. aeruginosa Clean Conditions Log Reduction 6.10 6.42 6.10 5.98 6.72 5.88 Pass/Fail PASS PASS PASS PASS PASS PASS Dirty Conditions Log Reduction 5.64 5.85 5.58 5.92 6.27 5.21 Pass/Fai PASS PASS PASS PASS PASS PASS 47 Table 14 - Results for 1500 D4L Test Organisms L. pneumophila E. coli S . aureus S. enteritidis L. monocytogeDcs P. aeruginosa Clean Conditions Log Reduction 6.88 7.14 6.98 6.52 7.39 6.45 Pass/Fai] PASS PASS PASS PASS PASS PASS Dirty Conditions Log Reduction 6.14 7.02 6.34 6.40 6.83 6.06 Pass/Fai PASS PASS PASS PASS PASS PASS Table 15 - Results for 2000 D4L Test Organisms L. pneumophila E. coli S. aureus S. enteritidis L. monocytogenes P. aeruginosa Clean Conditions Log Reduction 7.22 7.20 7.34 7.12 7.59 6.78 Pass/Fail PASS PASS PASS PASS PASS PASS Dirty Conditions Log Reduction 6.48 7.12 7.08 6.62 7.36 6.32 Pass/Fai PASS PASS PASS PASS PASS PASS Conclusion Three samples, 1000 D4L, 1500 D4L and 2000 D4L, passed the European Suspension Test at 10°C, for all of the microorganisms under test. Under identical conditions 500 D4L failed the test. A comparison of the results of the tests of Examples 9 and 10 shows that the composition "D4L" is more effective than the composition "LCF". The composition "D4L" includes anti-microbial agents that are more polar than 48 those included in the composition "LCF". Thus, the inclusion of polar antimicrobial agents increases the efficacy of the composition. Example 12 The dissociation of the Anti-microbial Composition "D4L" of Example 1 upon immersion in water This Example was conducted to assess the difference in biofilm growth between experimental and control surfaces after 48 hours submersion in water. The experimental surfaces were coated with the anti-microbial composition but the control surfaces were not. Method Eight aluminium bottles were painted with four different paint types, The paint types were Series 1 to 4, as set out below. Four of the bottles were painted with paint including 2% by weight of the anti-microbial composition of Example 1 and four were painted with standard paint and used as controls. The paint types were as follows: Series 1: K Type Gloss (tough, durable enamel for high quality industrial finishing and decorative interior/exterior woods). Series 2: Matt White Emulsion (interior/exterior decorative duties. Contains a preservative for in can protection against microbial spoilage). 49 Series 3: Blue Hydracoat (waterborne alternative to alkyd synthetic enamels "used for toy and model coating). Series 4: Aquaguard (two pack epoxy coatings for walls and floors). Each bottle was placed into an inoculated solution, covered and incubated for 48 hours. The bottles were then removed and sprayed with 0.5% Tween/phosphate"buffer solution (100 ml) to remove any biofilra that had formed. The resulting solutions were plated out making serial decimal dilutions, using the Miles and Misra Total Viable Count Technique and incubated inverted at 37°C overnight. The number of colony forming units (CFU) (taken to be viable bacterial individuals) was then counted and the experimental plates were compared to the controls. Results Biofilm growth recovered after 24 hours - E. coli Series 1: 50% more growth on experimental, compared to the control. Series 2: No growth on experimental., very small growth on control. Series 3: 25% more growth on experimental, compared to control. Series 4: 43% more growth on experimental, compared to the control. Biofilm growth recovered after 24 hours - Pseudomonas aeruginosa Series 1: 50% more growth on experimental, compared to the control. Series 2: No growth on control, very smaJl growth on experimental. Series 3: 25% more growth on experimental, compared to control. Series 4; 20% more growth on experimental, compared to the control. 50 Conclusion The components of the anti-microbial compositions dissociate and dissolve in the surrounding solution and provide a carbon source for the microbial populations. The results show an increase in growth of microorganisms on the treated materials after 24 hour immersion in microbial broth. This indicates a more nutrient rich environment in the paints including the antimicrobial composition of the invention compared to the controls and shows that the anti-microbial composition is biodegradable. Example 13 Low Rinsate Toxicology Method Four different surface coatings, paint series 1 to 4 as described in Example 12, were applied to aluminium panel substrates. These were then compared to controls that did not include the anti-microbial composition. The microorganisms used were E. coli and Pseudomonas aerogenosa. After 24 hours, the panels were rinsed with deionised water and the washings tested using Microtox Testing. This test uses a photoluminescent vibrio sp. (a bioluminous microbe) that is highly responsive to the effect of toxins. In the Microtox Test, a sample is mixed with living bacteria that are sensitive to the presence of toxic compounds. The mixture is allowed to regulate for a short time and then a light reading is taken. In the presence of substances at concentrations that are acutely toxic and which pose harm to humans, the bacteria are impaired and cease to give off light. Thus, the greater the light loss from a sample, the more toxic it is. 51 Results Figures 1 and 2 show the residual P. aeruginosa and E. cob" biofilm recovered after 24 hours, for the experimental and control samples respectively. It is clear from Figures 1 and 2 that the biofilm formation is greater for the control samples. Figures 3 and 4 show the toxicity of the surface rinsates for P. aeruginosa and E, coli, for the experimental and control samples respectively. Rinsates for the control samples are less toxic than for the experimental samples. However, the rinsates for both the experimental and the control samples showed low toxicity. Values for the effective concentration at which 50% of the population suffers from some adverse effect (EC50) were difficult to calculate and values for the lethal dose 50 (LD50) were not calculable. The rinsate of some of the anti-microbial compositions was similar in effect to that of the control of other test paints. For example, paint series 1 including the anti-microbial composition had similar effect on the test as paint series 3 without the composition of the invention. Thus, different products of similar types (i.e. paints) have different results in the Microtox test on the rinsate. This indicates that the anti-microbial composition of the invention only has a small effect on the rinsate toxicology, which is within the normal range for products that do not include anti-microbial agents. Thus, the rinsate has low toxicity and is safe. Low toxicity of rinsates from the compositions of the invention is highly desirable for the environment. 52 Example 14 Anti-micxobial Testing of Coated Panels Steel panels with a coating containing 0% (as a control), 1.5%, 2.0% and 3.0% by volume of the anti-microbial composition of Example 1 were tested. The microorganisms used were: Methicillin resistant staph aureus (MRSA) NCTC11940/2.1x105 CFU/ml Pseudornonas aeruginosa NCIMB12469/2.9xl05 CFU/ml Eschericia coli NCTC9001/2.2xl05 CFU/ral Method The selected microorganism (0.1 ml) was transferred to each of the coated panels. A sheet of sterile plastic (5 cm x 5 cm) was placed onto the microorganism, which was then carefully spread to fill the area covered by the plastic film. The first set of samples was processed immediately on completion of the above procedure, i.e. at 0 min. The plastic film was removed and placed onto a ceramic tile, ensuring that :he surface that had been in contact with the contaminated plate was ippermost. The exposed surface was then swabbed to remove all traces of the microorganism and the swab was transferred to 10 ml of maximum recovery diluent (MRD). A. second swab was used to swab the contaminated area on the surface of the panel. This swab was also transferred to the same vial of MRD and the vial was allowed to stand for 53 10 min to ensure that the swabs were well soaked. The vial was then whirlimixed thoroughly for 10 seconds. The resulting mixture (0.1 ml) was then transferred onto nutrient agar plates f and the plates were incubated at 37°C for 48 hours. The procedure was repeated on a further two sets of samples after a 30 min and 16 hour contact period at 25°C. Results The results of the tests undertaken on the coated panels are detailed in Table 16, 17 and 18. The results in Table 16 should be read as the base data with which the results obtained after 30 min and 16 hours are compared. However, it is clear from the variation is the results that recovery of the contaminating organisms is not entirely consistent. Table 16 - Time 0 min (counts per plate) Sample MRSA E. coli P. aeruginosa 1.5% 99 116 101 105 100 138 103 152 112 2.0% 79 100 107 98 110 86 100 97 136 3.0% 131 111 80 118 88 69 139 110 87 Control 102 90 122 142 108 127 115 107 128 54 Table 17 - Time 30 min (counts per plate) Sample MRSA E. coli P. aeruginosa 1.5% 9 16 2 7 4 12 18 11 16 2.0% 1 0 0 0 1 2 4 14 10 3.0% 0 0 0 1 3 0 5 9 5 Control 79 66 84 91 87 70 103 •97 311 Table 18 - Time 16 hours (counts per plate) Sample MRSA E. coli P. aeruginosa 1.5% 2 I 0 0 0 0 0 0 0 2.0% 0 0 0 0 0 0 0 0 0 3.0% 0 0 0 0 0 0 0 0 0 Control 65 44 51 39 41 22 79 59 61 Conclusion The results in Table 17 show that, even after 30 minutes contact, the antibacterial effect of the anti-microbial composition is evident. The consistently higher counts obtained from the control panel support this. In addition, there is a significant difference between the counts from the test 55 panel containing 1.5% by volume of the anti-microbial composition and from the test panels containing 2.0% and 3.0% by volume of the antimicrobial composition, again supporting the antibacterial effect of the composition of the invention. The counts detailed in Table 18 after 16 hours contact also endorse the antibacterial effect (_•_ the anti-microbinl composition although the consistently lower control figures suggest that there may have been a drying out effect during incubation. The results demonstrate that the anti-mi crobial composition of the invention confers an antibacterial effect on the panel coatings, giving the most effective kill at an inclusion rate 2.0% by volume or greater. WE CLAIM: 1. An anti-microbial composition used to reduce or control the formation of microbial colonies on or at a surface of a substrate to which the composition is applied consists essentially of: (i) at least one first anti-microbial agent having a surface tension of from 20 to 35 mN/m at 20°C and selected from (a) a quaternary ammonium compound having the general formula R1R2R3R4N+X- in which one or two of the R groups are alkyl substituted by aryl or interrupted by aryl or oxygen and the other R groups are the same or different and are C1 to C4 alkyl groups, (b) a dialkyldimethylammonium compound wherein the two non-methyl alkyl groups are selected from alkyl groups comprising from 8 to 12 carbon atoms, and (c) a benzalkonium halide or an aryl ring substituted benzalkonium halide; (ii) at least one compound which is a siloxane and has a surface tension of from 8 to 14 mN/m at 20°C; (iii) at least one polar solvent; and (iv) at least one additional anti-microbial agent; 2. An anti-microbial composition as claimed in claim 1, wherein the surface tension of the at least one compound (ii) is 10 mN/m. 3. An anti-microbial composition as claimed in claim 1 or 2, wherein the at least one compound (ii) is at least one polysiloxane. 4. An anti-microbial composition as claimed in claim 3, wherein the or each polysiloxane is selected from polydimethylsiloxanes, and polydimethylhydroxysiloxanes. 5. An anti-microbial composition as claimed in claim 4, wherein the or each polysiloxane is selected from polydimethylsiloxanes having a chain length of from C12 to C20 and polydimethylhydroxysiloxanes having a chain length of from C12 to C20. 6. An anti-microbial composition as claimed in any one of the preceding claims, wherein the at least one first anti-microbial agent and/or the at least one additional anti-microbial agent is of a polar nature. 7. An anti-microbial composition as claimed in any one of the preceding claims, comprising at least one anti-microbial agent selected from bacteriocidal, fungicidal, algicidal, yeasticidal and moldicidal agents. 8. An anti-microbial composition as claimed in any one of the preceding claims, wherein the first anti-microbial agent is selected from benzenemethanaminium N-dodecyl-N,N-dimethylchloride, and benzyl-Ci2-Ci6-alkyldimethyl-ammoniumchloride. 9. An anti-microbial composition as claimed in any one of claims 6 to 8, wherein the at least one additional anti-microbial agent is selected from amphoteric compounds, iodophores, phenolic compounds, quarternary ammonium compounds, hypochlorites and nitrogen based heterocyclic compounds. 10. An anti-microbial composition as claimed in claim 9, wherein the or each phenolic compound is selected from methyl, ethyl, butyl, halo and aryl substituted phenols. 11. An anti-microbial composition as claimed in claim 9, wherein the or each phenolic compound is selected from 2-phenylphenol, 2-benzyl-4-chlorophenol, 2-cyclopentanol-4-chlorophenol, 4-t-amylphenol, 4-t-butylphenol, 4-chloro-2-pentylphenol, 6-chloro-2-pentylphenol, p-chlorometa-xylenol, 2,4,4-trichloro-2-hydroxydiphenol, thymol, 2-i-propyl-3-methylphenol, chlorothymol, 3-methyl-4-chlorophenol, 2,6-dichloro-4-n-alkyl phenols, 2,4-dichloro-meta-xylenol, 2,4,6-trichlorophenol and 2-benzyl-4-chlorophenol. 12. An anti-microbial composition as claimed in claim 9, wherein the amphoteric compound is selected from dodecyl beta-alanine, dodecyl beta-aminobutyric acid, dodecylamino-di(aminoethylamino)glycine and N-(3-dodecylamino)propylglycine. 13. An anti-microbial composition as claimed in claim 9, wherein the or each iodophore is selected from a complex of iodine or triodine with polyvinylpyrrolidone, a polyether glycol, a polyvinyl alcohol, a polyacrylate, a polyamide, a polyalkylene and a polysaccharide. 14. An anti-microbial composition as claimed in claim 9, wherein the or each hypochlorite is selected from a hypochlorite of an alkali metal and an alkaline earth metal. 15. The anti-microbial composition as claimed in claim 14, wherein the hypochlorite is selected from a hypochlorite of lithium, sodium, potassium and calcium. 16. An anti-microbial composition as claimed in claim 1, comprising a chlorinated trisodium phosphate or a hydrate thereof, chlorine dioxide or a precursor thereof, N,N-dichloro-4-carboxybenzenesulphonamide, l,3-dichloro-5,5-dimethylhydantoin or a derivative of chloroisocyanuric acid. 17. An anti-microbial composition as claimed in claim 9, wherein the or each nitrogen based heterocyclic compound is selected from a pyridine derivative, a triazole, a thiazole and an imidazole. 18. An anti-microbial composition as claimed in claim 17, wherein the nitrogen based heterocyclic compound is selected from 4-pyridine carboxylic acid hydrazide, sodium 2-pyridinethiol and bis-(2-pyridylthio)zinc-1,1-dioxide. 19. An anti-microbial composition as claimed in any one of the preceding claims, comprising from 1 to 4% by volume of the at least one compound (ii). 20. An anti-microbial composition as claimed in any one of the preceding claims, wherein the at least one polar solvent is selected from water, isopropanol, diethylene glycol and dipropylene gycol. 21. An anti-microbial composition as claimed in any one of the preceding claims, comprising from 1 to 70% by volume of the polar solvent. 22. A formulation comprising the anti-microbial composition as claimed in any one of the preceding claims and a substrate selected from plastics, fibres, coatings, films, laminates, adhesives, sealants, clays, ceramics, concrete, sand, paints, varnishes, lacquers, cleaning agents and settable or curable compositions. 23. A formulation as claimed in claim 22, wherein the formulation comprises from 0.1 to 5.0% by weight of the anti-microbial composition. 24. A formulation as claimed in claim 23, wherein the formulation comprises from 0.5 to 2.0% by weight of the anti-microbial composition. 25. A method of manufacturing an anti-microbial composition as claimed in any one of claims 1 to 21, the method comprising the steps (i) mixing the anti¬microbial agents together, (ii) adding the at least one compound (ii) to the mixture of step (i), (iii) adding the polar solvent to the mixture of step (ii); and (iv) agitating the resulting mixture until a clear solution is formed.

Documents:

01031-delnp-2005-abstract.pdf

01031-delnp-2005-assignment.pdf

01031-delnp-2005-claims.pdf

01031-delnp-2005-correspondence-others.pdf

01031-delnp-2005-description (complete)-23-05-2008.pdf

01031-delnp-2005-description (complete)-24-07-2008.pdf

01031-delnp-2005-description (complete).pdf

01031-delnp-2005-drawings.pdf

01031-delnp-2005-form-1.pdf

01031-delnp-2005-form-18.pdf

01031-delnp-2005-form-2.pdf

01031-delnp-2005-form-3.pdf

01031-delnp-2005-form-5.pdf

01031-delnp-2005-form-6.pdf

01031-delnp-2005-gpa.pdf

01031-delnp-2005-pct-101.pdf

01031-delnp-2005-pct-210.pdf

01031-delnp-2005-pct-308.pdf

01031-delnp-2005-pct-409.pdf

01031-delnp-2005-pct-416.pdf

1031-DELNP-2003-Claims(27-12-2007).pdf

1031-DELNP-2003-Claims-(24-07-2008).pdf

1031-DELNP-2003-Claims-23-05-2008.pdf

1031-DELNP-2003-Correspondence-Others(27-12-2007).pdf

1031-DELNP-2003-Correspondence-Others-(24-07-2008).pdf

1031-DELNP-2003-Correspondence-Others-23-05-2008.pdf

1031-DELNP-2003-Drawings(27-12-2007).pdf

1031-DELNP-2003-Form-1(27-12-2007).pdf

1031-DELNP-2003-Form-1-23-05-2008.pdf

1031-DELNP-2003-Form-2(27-12-2007).pdf

1031-DELNP-2003-Form-3(27-12-2007).pdf

1031-DELNP-2003-Form-5(27-12-2007).pdf

1031-DELNP-2003-GPA(27-12-2007).pdf

1031-DELNP-2003-Petition-137-23-05-2008.pdf