Title of Invention	PROCESS FOR THE PREPARATION OF (3-CYANO-1H-INDOL-7-YL) (4-(4-FLUOROPHENETHYL) PIPERAZIN-1-YL)-METHANONE AND SALTS THEREOF.
Abstract	The present invention relates to a process for the preparation of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]-methanone and salts thereof, characterised in that an indole ester of the formula II in which R is as defined in Claim 1, is converter into 3-cyano-1H-indole-7-carboxylic acid via steps (1) to (4) according to Claim 1, and this is reacted with 1-[2-(4-fluorophenyl)ethyl] piperazine of salts thereof to give the product.

Title of Invention

PROCESS FOR THE PREPARATION OF (3-CYANO-1H-INDOL-7-YL) (4-(4-FLUOROPHENETHYL) PIPERAZIN-1-YL)-METHANONE AND SALTS THEREOF.

Abstract

The present invention relates to a process for the preparation of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]-methanone and salts thereof, characterised in that an indole ester of the formula II in which R is as defined in Claim 1, is converter into 3-cyano-1H-indole-7-carboxylic acid via steps (1) to (4) according to Claim 1, and this is reacted with 1-[2-(4-fluorophenyl)ethyl] piperazine of salts thereof to give the product.

Full Text	PROCESS FOR THE PREPARATION OF (3-CYANO-1H-INDOL-7-YL) (4-(4-FLUOROPHENETHYL) PIPERAZIN-1-YL)-METHANONE AND SALTS THEREOF The invention relates to a process for the preparation of (3-cyano-1H-indol- 7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]methanone of the formula I, and salts thereof, and of intermediates in the synthesis. The compound (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin- 1-yl]methanone and the corresponding physiologically acceptable salts surprisingly have selective affinity to 5-HT2A receptors. In particular, they are selective 5-HT2A antagonists. 5-HT2A antagonists exhibit clinically antipsychotic activity with no or minimal side effects and are correspondingly regarded as antipsychotics having few side effects. In addition, they can be used in the treatment of neurological disorders attributable to disturbances in serotonergic transmission, such as depression, anxiety states, panic illnesses, obsessive-compulsive disorders, pain, sleep disturbances, sleeplessness, eating disorders, such as anorexia nervosa, bulimia, addiction behaviour, dependence on certain addiction-causing substances, such as LSD and MDMA, cardiovascular disorders, such as various angina diseases, Raynaud"s syndrome, intermittent claudication, cardiac or peripheral vascular spasms, fibromyalgia, cardiac arrhythmia and thrombotic illnesses, since the sub- stances inhibit platelet aggregation. In combination with classical or atypical neuroleptics, the side effects induced by the neuroleptics can be sup- pressed. Owing to the reduction in ocular pressure, the substances can also be employed in glaucoma therapy. Toxic symptoms caused by poison- ing with, for example, ergovalin, can be suppressed using the substances. The compounds can therefore be used as medicament active ingredients in human and veterinary medicine. They can furthermore be used as interme- diates for the preparation of further medicament active ingredients. Since (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1 -yl]metha- none and salts thereof are very highly promising as medicaments, the preparation is of extremely high interest. The object of the present invention was therefore to find a novel and effect- ive synthesis variant for the 5-HT2A receptor antagonists. The invention therefore relates to a process for the preparation of (3-cyano- 1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]methanone of the formula I, and salts thereof, characterised in that (1) an indole ester of the formula II in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, is formylated, (2) the formyl ester of the formula III in which R is as defined above, formed from (1) is reacted with hydroxylamine to give an oxime derivative of the formula IV in which R is as defined above, (3) the oxime of the formula IV is converted into a cyanoindole ester of the formula V in which R has one of the meanings indicated above, (4) the ester of the formula V is saponified to give 3-cyano-1H-indole- 7-carboxylic acid, (5) the 3-cyano-1H-indole-7-carboxylic acid is reacted with 1-[2-(4-fluoro- phenyl)ethyl]piperazine or salts thereof to give the compound of the formula I, and (6) the resultant base of the formula I is converted into one of its salts by treatment with an acid. The substituent R in the formulae II to VI is an alkyl group having from 1 to 6 carbon atoms or an arylalkyl group. The alkyl group preferably has 1, 2, 3 or 4 carbon atoms and is therefore, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl or tert- butyl, furthermore also pentyl, 1-, 2- or 3-methylbutyl, 1,1-, 1,2- or 2,2- dimethylpropyl, 1-ethylpropyl, hexyl, 1-, 2-, 3- or 4-methylpentyl, 1,1-, 1,2-, 1,3-, 2,2-, 2,3- or 3,3-dimethylbutyl, 1- or 2-ethylbutyl, 1 -ethyl-1-methyl- propyl, 1-ethyl-2-methylpropyl, 1,1,2-or 1,2,2-trimethylpropyl, furthermore trifluoromethyl or pentafluoroethyl. Arylalkyl is alternatively -(CH2)0-Ar, where Ar is preferably phenyl or naphthyl, and o can be 0, 1 or 2. Arylalkyl is, in particular, benzyl, phenyl- ethyl or naphthylmethyl, particularly preferably benzyl. R is preferably methyl or ethyl, particularly preferably ethyl. The indole esters of the formula II are commercially available or can be prepared by known synthetic methods. The preparation can, for example, be carried out starting from 3-methyl-2-nitrobenzoic acid, which is com- mercially available, by the following reactions: (1) esterification of 3-methyl-2-nitrobenzoic acid, (2) reaction with an N,N-dimethylformamide acetal to give a 3-(2- dimethylaminovinyl)-2-nitrobenzoic acid ester, and (3) subsequent palladium-catalysed ring closure reaction to give the indole ester of the formula II. Suitable indole esters of the formula II are, in particular, methyl 1H-indole- 7-carboxylate, ethyl 1H-indole-7-carboxylate, tert-butyl 1H-indole- 7-carboxylate and benzyl 1H-indole-7-carboxylate. The synthesis according to the invention is particularly preferably carried out using ethyl 1H-indole-7- carboxylate. Suitable N,N-dimethylformamide acetals are, for example, N,N-dimethyl- formamide bis[2-(trimethylsilyl)ethyl] acetal, N,N-dimethylformamide dibenzyl acetal, N.N-dimethylformamide dibutyl acetal, N,N-dimethylform- amide di-tert-butyl acetal, N.N-dimethylforrnamide diethyl acetal, N,N- dimethylformamide diisopropyl acetal, N.N-dimethylformamide dimethyl acetal, N.N-dimethylformamide dineopentyl acetal, N.N-dimethylformamide dipropyl acetal and N.N-dimethylformamide ethylene acetal. Particular preference is given to N.N-dimethylformamide diethyl acetal and N,N-dimethylformamide dimethyl acetal. The palladium-catalysed cyclisation reaction is carried out analogously to the method of Leimgruber-Batcho [Clark R.D. et al, Heterocycles, 1984, 22, 195-221, Batcho D. et al, Organic Synthesis, 1985, 63, 214-225]. The reaction conditions selected are known from the literature. However, it is also possible to use other processes known from the literature, which are not explained in greater detail here, for the preparation of compounds of the formula II (lit.: Houben-Weyl, Methoden der Organ. Chemie [Methods of Organic Chemistry], Georg-Thieme-Verlag, Stuttgart). The formylation of a compound of the formula II to give a formyl ester of the formula III, in which R has one of the meanings indicated above, is carried out analogously to the method of Vilsmeyer-Haack [Jutz C. et al., Iminium Salts in Organic Chemistry Part I, New York, John Wiley & Sons Inc., 1976, pp. 234 ff, pp. 237 ff]. The reaction is preferably carried out in an aprotic polar solvent and at reaction temperatures between 0° and 50° and with heating to from 100° to 130°C. Particularly preferred solvents are dimethylformamide (DMF) and mixtures of DMF with aromatic hydrocarbons, such as benzene, toluene or xylene, or with further amides, such as N-methylpyrrolidone (NMP). The formylation is particularly preferably carried out in DMF in the presence of POCI3. Suitable formylindole esters of the formula III are, in particular, 7-methoxy- carbonyl-3-indolecarboxaldehyde, 7-ethoxycarbonyl-3-indolecarbox- aldehyde, 7-tert-butoxycarbonyl-3-indolecarboxaldehyde and 7-benzyloxy- carbonyl-3-indolecarboxaldehyde. The synthesis according to the invention is particularly preferably carried out using 7-ethoxycarbonyl-3-indole- carboxaldehyde. The oximation of the compounds of the formula III, as described above, is carried out under standard conditions (lit.: Kurtz P., Houben-Weyl, Methoden der Organ. Chemie [Methods of Organic Chemistry], Vol. VIII, Georg-Thieme-Verlag, Stuttgart). The oximation is particularly preferably carried out in polar aprotic solvents, such as DMF, benzene, toluene, xylene or NMP, at temperatures between 0° and 50°, in particular at room temperature. The preparation of the cyanoindole esters of the formula V, as described above, is carried out by treatment with acid. Suitable acids are, for example, inorganic acids, such as hydrochloric acid, sulfuric acid or phos- phoric acid, or organic acids, such as formic acid, acetic acid, p-toluene- sulfonic acid or methanesulfonic acid. The reaction is particularly preferably carried out in high-boiling aprotic solvents, such as DMF or NMP, or mixtures thereof with aprotic solvents, at temperatures between 20° and 100°, in particular at 50°C. In a particular embodiment of the process, steps (1) to (3) are carried out in situ, i.e. in a one-pot process analogously to Liebscher J. et al, Z. Chem. 1983, 23, 214-215, without the intermediates being isolated. The one-pot process gives better yields compared with the stepwise synthesis. The saponification of the compounds of the formula V to give 3-cyano-1H- indole-7-carboxylic acid is carried out under standard conditions (lit.: Houben-Weyl, Methoden der Organ. Chemie [Methods of Organic Chemistry], Georg-Thieme-Verlag, Stuttgart). The saponification is particularly preferably carried out using KOH in methanol at room temperature. As an alternative to chemical saponification, the ester cleavage can also be carried out enzymatically with the aid of esterases. Suitable esterases are, for example, Bacillus sp. esterase, Bacillus stearothermophilus esterase, Candida lipolytica esterase, Mucor miehei esterase, horse liver esterase, Saccaromyces cerevisiae esterase, pig"s liver esterase, Thermoanaerobium brockii esterase and pig"s liver esterase isoenzyme 1. The esterases may also be employed in immobilised form. Commercial immobilised esterases are, for example, pig"s liver esterase (PLE) immobilised on Eupergit® C or on oxirane-acrylic beads. Enzymatic reactions are preferably carried out in aqueous buffer systems, but other solvents, in particular alcohols, such as ethanol, may also be present. The reaction of 3-cyano-1 H-indole-7-carboxylic acid with 1-[2-(4-fluoro- phenyl)ethyl]piperazine or one of the salts, in particular, with 1-[2-(4-fluoro- phenyl)ethyl]piperazine dihydrochloride, is carried out by methods as are known from the literature for the acylation of amines [Houben-Weyl, I.c., Volume 15/11, pages 1 to 806 (1974)]. However, it is also possible to react the compounds in the presence of an inert solvent. Examples of suitable solvents are hydrocarbons, such as benzene, toluene and xylene; ketones, such as acetone and butanone; alcohols, such as methanol, ethanol, iso- propanol and n-butanol; ethers, such as tetrahydrofuran (THF) and diox- ane; amides, such as dimethylformamide (DMF) or N-methylpyrrolidone; nitriles, such as acetonitrile, if desired also mixtures of these solvents with one another or mixtures with water. The addition of an acid-binding agent, for example an alkali or alkaline earth metal hydroxide, carbonate or bicarbonate or of another salt of a weak acid of the alkali or alkaline earth metals, preferably of potassium, sodium or calcium, or the addition of an organic base, such as triethylamine, dimethylaniline, pyridine or quinoline, or of an excess of a piperazine derivative, may be favourable. Depending on the conditions used, the reaction temperature is between about 0° and 150°, normally between 20° and 130°. Instead of the 3-cyano-1H-indole-7-carboxylic acid, it is also possible to employ derivatives of this acid, preferably the preactivated carboxylic acid, or a corresponding carboxylic acid halide, a symmetrical or mixed anhydride or an active ester of 3-cyano-1H-indole-7-carboxylic acid. Radicals of this type for activation of the carboxyl group in typical acylation reactions are described in the literature (for example in the standard works, such as Houben-Weyl, Methoden der organischen Chemie [Methods of Organic Chemistry], Georg-Thieme-Verlag, Stuttgart). Activated esters are advantageously formed in situ, for example by addition of HOBt or N-hydroxysuccinimide. The compound 1-[2-(4-fluorophenyl)ethyl]piperazine and salts thereof are known and can be prepared by conventional processes which are known to the person skilled in the art. A description of the preparation is disclosed, for example, in DE 2855703. A resultant base of the formula I can be converted into the associated acid- addition salt using an acid. Suitable acids for this reaction are those which give physiologically acceptable salts. Thus, it is possible to use inorganic acids, for example sulfuric acid, hydrohalic acids, such as hydrochloric acid or hydrobromic acid, phosphoric acids, such as orthophosphoric acid, nitric acid or sulfamic acid, furthermore organic acids, in detail aliphatic, alicyclic, araliphatic, aromatic or heterocyclic monobasic or polybasic carboxylic, sulfonic or sulfuric acids, such as formic acid, acetic acid, propionic acid, pivalic acid, diethylacetic acid, malonic acid, succinic acid, pimelic acid, fumaric acid, maleic acid, lactic acid, tartaric acid, malic acid, benzoic acid, salicylic acid, 2-phenylpropionic acid, citric acid, gluconic acid, ascorbic acid, nicotinic acid, isonicotinic acid, methane- or ethanesulfonic acid, ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, naphthalenemono- and -disulfonic acids and laurylsulfuric acid. In a preferred embodiment, the salt formation is carried out in a solvent mixture of acetone/water in a ratio of between 5:1 and 4:1 by precipitation using hydrochloric acid (37%). (3-Cyano-1H-indol-7-yl)[4-(4-fIuorophen- ethyl)piperazin-1-yl]methanone, hydrochloride, is formed. In a further embodiment of the process according to the invention, the cyanoindole ester of the formula V can be reacted directly, without prior saponification to 3-cyano-1H-indole-7-carboxylic acid, with 1-[2-(4-fluoro- phenyl)ethyl]piperazine or a corresponding salt by chemical or biochemical aminolysis. The chemical aminolysis can be carried out, for example, by the method of Menger F. M. et al., J. Am. Chem. Soc. 1969, 91, 5346-9. The biochemical aminolysis can be carried out, for example, by reaction of a compound of the formula V with 1-[2-(4-fluorophenyl)ethyl]piperazine or one of the corresponding salts in the presence of a lipase or an antibody. The biochemical aminolysis can be carried out, for example, by the method of Gotor V. et al., Bioorg. Med. Chem. 1999, 7, 2189-2197. The invention therefore likewise relates to a process for the preparation of (3-cyano-1 H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1 -yl]methanone of the formula I and salts thereof, characterised in that (1) an indole ester of the formula II in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, isformylated, (2) the formyl ester of the formula III in which R is as defined above, formed from (1) is reacted with hydroxylamine to give an oxime derivative of the formula IV in which R is as defined above, (3) the oxime of the formula IV is converted into a cyanoindole ester of the formula V in which R has one of the meanings indicated above, (4) the ester of the formula V is converted into the compound of the formula I by aminolysis using 1-[2-(4-fluorophenyl)ethyl]piperazine or one of the salts, and (5) the resultant base of the formula I is converted into one of its salts by treatment with an acid. In a further embodiment of the process according to the invention, the compound 3-cyano-1 H-indole-7-carboxylic acid can be prepared by halogenation of an indole ester of the formula II as described above to give a compound of the formula VI in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl and Hal is CI, Br or l, followed by cyanation. The halogenation of the compounds of the formula II, as described above, is carried out under standard conditions (lit.: Houben-Weyl, Methoden der Organ. Chemie [Methods of Organic Chemistry], Georg-Thieme-Verlag, Stuttgart) or analogously to Heterocycles, 1986, 24. 2879-85, ibid. 1989, 29,1663-7; J. Am. Chem. Soc. 1985, 107, 2943-5; J. Org. Chem. 1993, 58, 2058-60, or J. Chem. Soc, Perkin Trans. 1, 1989, 2009-15. The bromination or iodination in the 3-position of the indole can likewise be carried out analogously to Bocchi et al. Synthesis 1982, 1096-1097. Examples of suitable solvents for the halogenation are hydrocarbons, such as benzene, toluene and xylene; halogenated hydrocarbons, such as dichloromethane and chloroform; ketones, such as acetone and butanone; alcohols, such as methanol, ethanol, isopropanol and n-butanol; ethers, such as tetrahydrofuran (THF) and dioxane; amides, such as dimethyl- formamide (DMF) and N-methylpyrrolidone; nitriles, such as acetonitrile, and if desired mixtures of these solvents with one another. Compounds prepared in accordance with the invention by halogenation are, for example, methyl 3-chloro-1H-indole-7-carboxylate, methyl 3-bromo- 1H-indole-7-carboxylate, methyl 3-iodo-1H-indole-7-carboxylate, ethyl 3- chloro-1H-indole-7-carboxylate, ethyl 3-bromo-1H-indole-7-carboxylate, ethyl 3-iodo-1H-indole-7-carboxylate, tert-butyl 3-chloro-1H-indo!e-7- carboxylate, tert-butyl 3-bromo-1H-indole-7-carboxylate, tert-butyl 3-iodo- 1H-indole-7-carboxylate, benzyl 3-chloro-1H-indole-7-carboxylate, benzyl 3- bromo-1H-indole-7-carboxylate and benzyl 3-iodo-1H-indole-7-carboxylate. The use of ethyl 3-bromo-1H-indole-7-carboxylate or ethyl 3-iodo-1H- indole-7-carboxylate is particularly suitable in accordance with the invention. The substitution of the halogen group of the compounds of the formula VI by the cyano group is carried out analogously to the method of Cassar L. et al., Adv. Chem. Ser. 1974, 132, 252-73, with nickel catalysis or analogously to the method of Sakamoto T. et al, J. Chem. Soc, Perkin Trans. 1 1999, 16, 2323-2326, or Chatani N. et al, J. Org. Chem. 1986, 51, 4714-16, with palladium catalysis. The cyano group is particularly preferably introduced with palladium cataly- sis. The invention therefore likewise relates to a process for the preparation of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1 -yl]methanone of the formula I and salts thereof, characterised in that (1) an indole ester of the formula II in which R is an alky! group having from 1 to 6 carbon atoms or arylalkyl, is halogenated, (2) the halogen group of the ester of the formula VI in which R and Hal are as defined above, formed from (1) is converted into a cyano group, with the ester being saponified to 3-cyano-1H-indole-7-carboxylic acid at the same time, (3) the 3-cyano-1H-indole-7-carboxylic acid is reacted with 1-[2-(4-fluoro- phenyl)ethyl]piperazine or salts thereof to give the compound of the formula I, and (4) the resultant base of the formula I is converted into one of its salts by treatment with an acid. A further aspect of the invention relates to compounds of the formula IV in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and salts thereof. Alkyl and arylalkyl have one of the meanings indicated above. Compounds of the formula IV may occur in two isomeric forms, compounds of the formulae IVa and IVb. The general formula IV covers the individual isomers of the formulae IVa and IVb as well as mixtures thereof. in which R has one of the meanings indicated above. Preferred compounds of the formula IV are methyl 3-hydroxyimino-1H-indole-7-carboxylate, ethyl 3-hydroxyimino-1H-indole-7-carboxylate, tert-butyl 3-hydroxyimino-1H-indole-7-carboxylate and benzyl 3-hydroxyimino-1H-indole-7-carboxylate, where both the Z- and E- forms and mixtures of these are included. Particularly preferred compounds of the formula IV are ethyl (2)-3-hydroxyimino-1H-indole-7-carboxylate, ethyl (E)-3-hydroxyimino-1H-indole-7-carboxylate and E/Z mixtures. A salt of the compounds of the formula IV can be prepared by the methods described above for compounds of the formula I. The compounds of the formula IV are valuable intermediates in the synthe- sis of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]metha- none and salts thereof, as described above. A further aspect of the invention relates to compounds of the formula V in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and salts thereof. Alkyl and arylalkyl have one of the meanings indicated above. Preferred compounds of the formula V are methyl 3-cyano-1H-indole-7-carboxylate, ethyl 3-cyano-1H-indole-7-carboxylate, tert-butyl 3-cyano-1H-indole-7-carboxylate and benzyl 3-cyano-1H-indole-7-carboxylate, and salts thereof. The process according to the invention is particularly preferably carried out using ethyl 3-cyano-1H-indole-7-carboxylate. A salt of the compounds of the formula V can be prepared by the methods described above for compounds of the formula I. The compounds of the formula V are valuable intermediates in the synthe- sis of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]metha- none and salts thereof, as described above. The invention likewise relates to the compound 3-cyano-1H-indole-7-car- boxylic acid and salts thereof. A further aspect of the invention relates to compounds of the formula VI in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and Hal is Cl, Br or I and salts thereof. Alkyl and arylalkyl have one of the meanings indicated above. Preferred compounds of the formula VI are methyl 3-bromo-1H-indole-7-carboxylate, ethyl 3-bromo-1H-indole-7-carboxylate, tert-butyl 3-bromo-1H-indole-7-carboxylate and benzyl 3-bromo-1H-indole-7-carboxylate, methyl 3-iodo-1H-indole-7-carboxylate, ethyl 3-iodo-1H-indole-7-carboxylate, tert-butyl 3-iodo-1H-indole-7-carboxylate and benzyl 3-iodo-1H-indole-7-carboxylate, and salts thereof. The process according to the invention is particularly preferably carried out using ethyl 3-bromo-1H-indole-7-carboxylate or ethyl 3-iodo-1H-indole-7- carboxylate. A salt of the compounds of the formula VI can be prepared by the methods described above for compounds of the formula I. The compounds of the formula VI are valuable intermediates in the synthe- sis of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]metha- none and salts thereof, as described above. Even without further details, it is assumed that a person skilled in the art will be able to utilise the above description in the broadest scope. The preferred embodiments should therefore merely be regarded as descriptive disclosure which is absolutely not to be regarded as limiting in any way. All temperature data above and below are given in °C. In the following examples, "conventional work-up" means that water is added if necessary, the mixture is adjusted, if necessary, to a pH of between 2 and 10, depending on the constitution of the end product, the mixture is extracted with ethyl acetate or dichloromethane, the phases are separated, the organic phase is dried over sodium sulfate and evaporated, and the product is purified by chromatography on silica gel and/or by crystallisation. Example 1: 1.1. Methyl 3-formylindole-7-carboxylate 2.9 ml of phosphorus oxychloride are added slowly to 7 ml of N,N-dimethyl- formamide in a nitrogen atmosphere (formylation solution). 5 g (0.029 mol) of methyl indole-7-carboxy!ate are dissolved in 7 ml of DMF and added slowly to the formylation solution, during which the temperature does not rise above 30 degrees. The mixture is then warmed at 100° for one hour. After cooling, the mixture is poured into water and neutralised using sodium hydroxide solution, and the deposited crystals are filtered off with suction, m.p. 154°. Yield 5.3 g (89.9% of theory). 1.2. Methyl 3-(hydroxyiminomethyl)indole-7-carboxylate 5 g of methyl 3-formylindole-7-carboxylate (0.024 mol) are added to a solu- tion of 0.03 mol of hydroxylammonium hydrochloride in dimethylformamide. The reaction mixture is heated at 125° for one hour and subjected to con- ventional work-up, giving 5.1 g of methyl 3-(hydroxyiminomethyl)indole-7- carboxylate. 1.3. Methyl 3-cyanoindole-7-carboxylate 5 g of methyl 3-(hydroxyiminomethyl)indole-7-carboxylate are suspended in 20 ml of toluene, 1 ml of sulfonyl chloride is added, and the mixture is refluxed for one hour. Evaporation and extraction with ethyl acetate give 4.5 g of methyl cyanoindole-7-carboxylate, m.p. 212°. 1.4. 3-Cyanoindole-7-carboxylic acid 4.5 g (0.022 mol) of methyl cyanoindole-7-carboxylate are suspended in 100 ml of methanol, and a solution of 30 ml of sodium hydroxide solution (w = 32%) in 30 ml of water is added at room temperature. Stirring over- night gives a virtually clear solution, which is filtered and evaporated. Water is added to the residue until a clear solution is formed, and the mixture is adjusted to pH = 2 using concentrated hydrochloric acid with ice-cooling. The white crystals are filtered off with suction and dried for 2 hours under reduced pressure, giving 4 g of 3-cyanoindole-7-carboxylic acid (97.7% of theory); m.p. 317.5 - 318.5°. 1.5. 7-{4-[2-(4-FIuorophenyl)ethyl]piperazine-1 -carbonyl}-1H-indole- 3-carbonitrile 5 g (0.027 mol) of 3-cyanoindole-7-carboxylic acid are dissolved in 40 ml of hot N-methylpyrrolidone, the solution is cooled to 40°, and 7.6 g (0.027 mol) of N,N-carbonyldiimidazole are added. The mixture is stirred at room temperature for a further one hour. A suspension of 1-(2-(4-fluoro- phenyl)ethyl)piperazine dihydrochloride in 40 ml of N-methylpyrrolidone is subsequently poured in. After 5 minutes, a clear solution is formed and just afterwards white crystals deposit. The mixture is stirred overnight at room temperature. The crystals are filtered off with suction, washed and dried, giving 5 g of 7-{4-[2-(4-fluorophenyl)ethyl]piperazin-1-carbonyl}-1H-indole- 3-carbonitrile as the free base having a melting point (m.p.) of 192.0 - 193.5°. The chemical names 7-{4-[2-(4-fluorophenyl)ethyl]piperazine-1-carbonyl}- 1H-indole-3-carbonitrile and (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)- piperazin-1-yl]methanone are synonymous. 1.6. 7-{4-[2-(4-Fluorophenyl)ethyl]piperazin-1 -carbonyl}-1H-indole- 3-carbonitrile, hydrochloride 2.1g of the free base obtained in accordance with 1.5 are heated in 50 ml of acetone, and water is added until a clear solution is formed. A mixture of 0.6 ml of hydrochloric acid (w = 37%) and 1.2 ml of acetone is then stirred in. The mixture is subsequently evaporated to half the volume in a rotary evaporator. The precipitated hydrochloride is filtered off with suction, washed with acetone and diethyl ether and dried, giving 1.6 g of 7-{4-[2-(4- fluorophenyl)ethyl]piperazin-1-carbonyl}-1H-indole-3-carbonitrile, hydro- chloride (69% of theory), decomposition range 314 - 319°. Example 2: 2.1. Methyl 3-cyanoindole-7-carboxylate 9.1 g of phosphoryl chloride are added to 30 ml of dimethylformamide with ice cooling at a reaction temperature of 20-30°. A solution of 8 g of methyl indole-7-carboxylate in dimethylformamide is added dropwise, during which the temperature rises to 40°. After one hour at 125°, the solution is added dropwise while still hot to a solution of 6.3 g of hydroxylammonium chloride in 40 ml of dimethylformamide, and the mixture is stirred at 120°C for a fur- ther 15 minutes. The mixture is poured into water, extracted with ethyl ace- tate, filtered through neutral aluminium oxide and evaporated, giving 4.5 g of methyl 3-cyanoindote-7-carboxylate having a melting range of 212 - 213.5° (48.9% of theory). The further reaction is carried out analogously to Example 1.4. to 1.6. Example 3: 3.1. Ethyl 3-bromoindole-7-carboxylate 12 g of pyridine hydrobromide perbromide are added to a solution of 5 g of ethyl indole-7-carboxylate in 50 g of pyridine. The reaction mixture is warmed to 30-50° and stirred until the conversion is complete (from about 3 to 10 hours). Conventional work-up gives ethyl 3-bromoindole-7-carboxylate. 3.2. 3-Cyanoindole-7-carboxylic acid 7 g of ethyl 3-bromoindole-7-carboxylate are dissolved in 70 g of NMP, and 4 g of CuCN are added. The mixture is heated to 100-140°C with stirring. After 3 hours, the mixture is subjected to conventional work-up, giving 3-cyanoindole-7-carboxylic acid. The further reaction of the 3-cyanoindole-7-carboxylic acid is carried out analogously to Example 1.5. to 1.6. WE CLAIM : 1. Process for the preparation of (3-cyano-1H-indol-7-yl)[4-(4-fluorophen- ethyl)piperazin-1-yl]methanone of the formula I and salts thereof, characterised in that (1) an indole ester of the formula II in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, is formylated, (2) the formyl ester of the formula III in which R is as defined above, formed from (1) is reacted with hydroxylamine to give an oxime deriva- tive of the formula IV in which R is as defined above, (3) the oxime of the formula IV is converted into a cyanoindole ester of the formula V in which R has one of the meanings indicated above, (4) the ester of the formula V is saponified to give 3-cyano-1H-indole-7- carboxylic acid, (5) the 3-cyano-1H-indole-7-carboxylic acid is reacted with 1-[2-(4- fluorophenyl)ethyl]piperazine or salts thereof to give the compound of the formula I, and (6) the resultant base of the formula I is converted into one of its salts by treatment with an acid. Process as claimed in Claim 1 wherein steps (1) to (3) are carried out as a one-pot synthesis. 3. Process as claimed in Claim 1 where in step (4), the ester of the formula V is converted into the compound of the formula I by aminolysis using 1-[2-(4-fluorophenyl)ethyl]piperazine or one of the salts. 4. Process as claimed in Claim 1 wherein 3-cyano-1H-indole- 7-carboxylic acid is prepared by halogenation of an indole ester of the formula II in accordance with Claim 1 to give a compound of the formula VI in which R is an alkyl group having from 1 to 6 carbon atoms or aryl- alkyl, and Hal is CI, Br or l, followed by cyanation. 5. Process as claimed in one or more of Claims 1 to 4, wherein R is ethyl. 6. Process as claimed in one or more of Claims 1 to 5, wherein that 1-[2-(4-fluorophenyl)ethyl]piperazine is employed as the dihydro- chloride. 7. Process as claimed in one or more of Claims 1 to 6, wherein that the base (3-cyano-1H-indoI-7-yl)[4-(4-fluorophenethyl)piperazin-1 - yl]methanone is converted into its hydrochloride. 8. Oxime derivatives of the formula IV in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and salts thereof. 9. Cyanoindole esters of the formula V in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and salts thereof. 10. 3-Cyano-1H-indole-7-carboxylic acid and salts thereof. 11. Compounds of the formula VI in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and Hal is Cl, Br or I, and salts thereof. The present Invention relates to a process for the preparation of (3-cyano-lH-Indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl)] methanone and salts thereof, characterized in that an Indole ester of the formula II in which R is as defined in claim1, is converted into 3-cyano-1H-indole-7-carboxylic acid via steps (1) to (4) according to Claim 1, and this Is reacted with 1-[2-(4- fluorophenyl)ethyl]piperazine of salts thereof to give the product.

Full Text

PROCESS FOR THE PREPARATION OF (3-CYANO-1H-INDOL-7-YL) (4-(4-FLUOROPHENETHYL)
PIPERAZIN-1-YL)-METHANONE AND SALTS THEREOF
The invention relates to a process for the preparation of (3-cyano-1H-indol-
7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]methanone of the formula I,
and salts thereof, and of intermediates in the synthesis.
The compound (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-
1-yl]methanone and the corresponding physiologically acceptable salts
surprisingly have selective affinity to 5-HT2A receptors. In particular, they
are selective 5-HT2A antagonists.
5-HT2A antagonists exhibit clinically antipsychotic activity with no or minimal
side effects and are correspondingly regarded as antipsychotics having few
side effects. In addition, they can be used in the treatment of neurological
disorders attributable to disturbances in serotonergic transmission, such as
depression, anxiety states, panic illnesses, obsessive-compulsive
disorders, pain, sleep disturbances, sleeplessness, eating disorders, such
as anorexia nervosa, bulimia, addiction behaviour, dependence on certain
addiction-causing substances, such as LSD and MDMA, cardiovascular
disorders, such as various angina diseases, Raynaud"s syndrome,
intermittent claudication, cardiac or peripheral vascular spasms,
fibromyalgia, cardiac arrhythmia and thrombotic illnesses, since the sub-
stances inhibit platelet aggregation. In combination with classical or atypical
neuroleptics, the side effects induced by the neuroleptics can be sup-
pressed. Owing to the reduction in ocular pressure, the substances can
also be employed in glaucoma therapy. Toxic symptoms caused by poison-
ing with, for example, ergovalin, can be suppressed using the substances.
The compounds can therefore be used as medicament active ingredients in
human and veterinary medicine. They can furthermore be used as interme-
diates for the preparation of further medicament active ingredients.
Since (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1 -yl]metha-
none and salts thereof are very highly promising as medicaments, the
preparation is of extremely high interest.
The object of the present invention was therefore to find a novel and effect-
ive synthesis variant for the 5-HT2A receptor antagonists.
The invention therefore relates to a process for the preparation of (3-cyano-
1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]methanone of the formula
I,
and salts thereof, characterised in that
(1) an indole ester of the formula II
in which
R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
is formylated,
(2) the formyl ester of the formula III
in which R is as defined above,
formed from (1) is reacted with hydroxylamine to give an oxime derivative
of the formula IV
in which R is as defined above,
(3) the oxime of the formula IV is converted into a cyanoindole ester of the
formula V
in which R has one of the meanings indicated above,
(4) the ester of the formula V is saponified to give 3-cyano-1H-indole-
7-carboxylic acid,
(5) the 3-cyano-1H-indole-7-carboxylic acid is reacted with 1-[2-(4-fluoro-
phenyl)ethyl]piperazine or salts thereof to give the compound of the
formula I, and
(6) the resultant base of the formula I is converted into one of its salts by
treatment with an acid.
The substituent R in the formulae II to VI is an alkyl group having from 1 to
6 carbon atoms or an arylalkyl group.
The alkyl group preferably has 1, 2, 3 or 4 carbon atoms and is therefore,
for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl or tert-
butyl, furthermore also pentyl, 1-, 2- or 3-methylbutyl, 1,1-, 1,2- or 2,2-
dimethylpropyl, 1-ethylpropyl, hexyl, 1-, 2-, 3- or 4-methylpentyl, 1,1-, 1,2-,
1,3-, 2,2-, 2,3- or 3,3-dimethylbutyl, 1- or 2-ethylbutyl, 1 -ethyl-1-methyl-
propyl, 1-ethyl-2-methylpropyl, 1,1,2-or 1,2,2-trimethylpropyl, furthermore
trifluoromethyl or pentafluoroethyl.
Arylalkyl is alternatively -(CH2)0-Ar, where Ar is preferably phenyl or
naphthyl, and o can be 0, 1 or 2. Arylalkyl is, in particular, benzyl, phenyl-
ethyl or naphthylmethyl, particularly preferably benzyl.
R is preferably methyl or ethyl, particularly preferably ethyl.
The indole esters of the formula II are commercially available or can be
prepared by known synthetic methods. The preparation can, for example,
be carried out starting from 3-methyl-2-nitrobenzoic acid, which is com-
mercially available, by the following reactions:
(1) esterification of 3-methyl-2-nitrobenzoic acid,
(2) reaction with an N,N-dimethylformamide acetal to give a 3-(2-
dimethylaminovinyl)-2-nitrobenzoic acid ester, and
(3) subsequent palladium-catalysed ring closure reaction to give the
indole ester of the formula II.
Suitable indole esters of the formula II are, in particular, methyl 1H-indole-
7-carboxylate, ethyl 1H-indole-7-carboxylate, tert-butyl 1H-indole-
7-carboxylate and benzyl 1H-indole-7-carboxylate. The synthesis according
to the invention is particularly preferably carried out using ethyl 1H-indole-7-
carboxylate.
Suitable N,N-dimethylformamide acetals are, for example, N,N-dimethyl-
formamide bis[2-(trimethylsilyl)ethyl] acetal, N,N-dimethylformamide
dibenzyl acetal, N.N-dimethylformamide dibutyl acetal, N,N-dimethylform-
amide di-tert-butyl acetal, N.N-dimethylforrnamide diethyl acetal, N,N-
dimethylformamide diisopropyl acetal, N.N-dimethylformamide dimethyl
acetal, N.N-dimethylformamide dineopentyl acetal, N.N-dimethylformamide
dipropyl acetal and N.N-dimethylformamide ethylene acetal.
Particular preference is given to N.N-dimethylformamide diethyl acetal and
N,N-dimethylformamide dimethyl acetal.
The palladium-catalysed cyclisation reaction is carried out analogously to
the method of Leimgruber-Batcho [Clark R.D. et al, Heterocycles, 1984, 22,
195-221, Batcho D. et al, Organic Synthesis, 1985, 63, 214-225].
The reaction conditions selected are known from the literature. However, it
is also possible to use other processes known from the literature, which are
not explained in greater detail here, for the preparation of compounds of
the formula II (lit.: Houben-Weyl, Methoden der Organ. Chemie [Methods of
Organic Chemistry], Georg-Thieme-Verlag, Stuttgart).
The formylation of a compound of the formula II to give a formyl ester of
the formula III, in which R has one of the meanings indicated above, is
carried out analogously to the method of Vilsmeyer-Haack [Jutz C. et al.,
Iminium Salts in Organic Chemistry Part I, New York, John Wiley & Sons
Inc., 1976, pp. 234 ff, pp. 237 ff]. The reaction is preferably carried out in
an aprotic polar solvent and at reaction temperatures between 0° and 50°
and with heating to from 100° to 130°C. Particularly preferred solvents are
dimethylformamide (DMF) and mixtures of DMF with aromatic
hydrocarbons, such as benzene, toluene or xylene, or with further amides,
such as N-methylpyrrolidone (NMP).
The formylation is particularly preferably carried out in DMF in the presence
of POCI3.
Suitable formylindole esters of the formula III are, in particular, 7-methoxy-
carbonyl-3-indolecarboxaldehyde, 7-ethoxycarbonyl-3-indolecarbox-
aldehyde, 7-tert-butoxycarbonyl-3-indolecarboxaldehyde and 7-benzyloxy-
carbonyl-3-indolecarboxaldehyde. The synthesis according to the invention
is particularly preferably carried out using 7-ethoxycarbonyl-3-indole-
carboxaldehyde.
The oximation of the compounds of the formula III, as described above, is
carried out under standard conditions (lit.: Kurtz P., Houben-Weyl,
Methoden der Organ. Chemie [Methods of Organic Chemistry], Vol. VIII,
Georg-Thieme-Verlag, Stuttgart).
The oximation is particularly preferably carried out in polar aprotic solvents,
such as DMF, benzene, toluene, xylene or NMP, at temperatures between
0° and 50°, in particular at room temperature.
The preparation of the cyanoindole esters of the formula V, as described
above, is carried out by treatment with acid. Suitable acids are, for
example, inorganic acids, such as hydrochloric acid, sulfuric acid or phos-
phoric acid, or organic acids, such as formic acid, acetic acid, p-toluene-
sulfonic acid or methanesulfonic acid. The reaction is particularly preferably
carried out in high-boiling aprotic solvents, such as DMF or NMP, or
mixtures thereof with aprotic solvents, at temperatures between 20° and
100°, in particular at 50°C.
In a particular embodiment of the process, steps (1) to (3) are carried out in
situ, i.e. in a one-pot process analogously to Liebscher J. et al, Z. Chem.
1983, 23, 214-215, without the intermediates being isolated. The one-pot
process gives better yields compared with the stepwise synthesis.
The saponification of the compounds of the formula V to give 3-cyano-1H-
indole-7-carboxylic acid is carried out under standard conditions (lit.:
Houben-Weyl, Methoden der Organ. Chemie [Methods of Organic
Chemistry], Georg-Thieme-Verlag, Stuttgart).
The saponification is particularly preferably carried out using KOH in
methanol at room temperature.
As an alternative to chemical saponification, the ester cleavage can also be
carried out enzymatically with the aid of esterases. Suitable esterases are,
for example, Bacillus sp. esterase, Bacillus stearothermophilus esterase,
Candida lipolytica esterase, Mucor miehei esterase, horse liver esterase,
Saccaromyces cerevisiae esterase, pig"s liver esterase,
Thermoanaerobium brockii esterase and pig"s liver esterase isoenzyme 1.
The esterases may also be employed in immobilised form. Commercial
immobilised esterases are, for example, pig"s liver esterase (PLE)
immobilised on Eupergit® C or on oxirane-acrylic beads.
Enzymatic reactions are preferably carried out in aqueous buffer systems,
but other solvents, in particular alcohols, such as ethanol, may also be
present.
The reaction of 3-cyano-1 H-indole-7-carboxylic acid with 1-[2-(4-fluoro-
phenyl)ethyl]piperazine or one of the salts, in particular, with 1-[2-(4-fluoro-
phenyl)ethyl]piperazine dihydrochloride, is carried out by methods as are
known from the literature for the acylation of amines [Houben-Weyl, I.c.,
Volume 15/11, pages 1 to 806 (1974)]. However, it is also possible to react
the compounds in the presence of an inert solvent. Examples of suitable
solvents are hydrocarbons, such as benzene, toluene and xylene; ketones,
such as acetone and butanone; alcohols, such as methanol, ethanol, iso-
propanol and n-butanol; ethers, such as tetrahydrofuran (THF) and diox-
ane; amides, such as dimethylformamide (DMF) or N-methylpyrrolidone;
nitriles, such as acetonitrile, if desired also mixtures of these solvents with
one another or mixtures with water. The addition of an acid-binding agent,
for example an alkali or alkaline earth metal hydroxide, carbonate or
bicarbonate or of another salt of a weak acid of the alkali or alkaline earth
metals, preferably of potassium, sodium or calcium, or the addition of an
organic base, such as triethylamine, dimethylaniline, pyridine or quinoline,
or of an excess of a piperazine derivative, may be favourable. Depending
on the conditions used, the reaction temperature is between about 0° and
150°, normally between 20° and 130°.
Instead of the 3-cyano-1H-indole-7-carboxylic acid, it is also possible to
employ derivatives of this acid, preferably the preactivated carboxylic acid,
or a corresponding carboxylic acid halide, a symmetrical or mixed
anhydride or an active ester of 3-cyano-1H-indole-7-carboxylic acid.
Radicals of this type for activation of the carboxyl group in typical acylation
reactions are described in the literature (for example in the standard works,
such as Houben-Weyl, Methoden der organischen Chemie [Methods of
Organic Chemistry], Georg-Thieme-Verlag, Stuttgart).
Activated esters are advantageously formed in situ, for example by addition
of HOBt or N-hydroxysuccinimide.
The compound 1-[2-(4-fluorophenyl)ethyl]piperazine and salts thereof are
known and can be prepared by conventional processes which are known to
the person skilled in the art. A description of the preparation is disclosed,
for example, in DE 2855703.
A resultant base of the formula I can be converted into the associated acid-
addition salt using an acid. Suitable acids for this reaction are those which
give physiologically acceptable salts. Thus, it is possible to use inorganic
acids, for example sulfuric acid, hydrohalic acids, such as hydrochloric acid
or hydrobromic acid, phosphoric acids, such as orthophosphoric acid, nitric
acid or sulfamic acid, furthermore organic acids, in detail aliphatic, alicyclic,
araliphatic, aromatic or heterocyclic monobasic or polybasic carboxylic,
sulfonic or sulfuric acids, such as formic acid, acetic acid, propionic acid,
pivalic acid, diethylacetic acid, malonic acid, succinic acid, pimelic acid,
fumaric acid, maleic acid, lactic acid, tartaric acid, malic acid, benzoic acid,
salicylic acid, 2-phenylpropionic acid, citric acid, gluconic acid, ascorbic
acid, nicotinic acid, isonicotinic acid, methane- or ethanesulfonic acid,
ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid,
p-toluenesulfonic acid, naphthalenemono- and -disulfonic acids and
laurylsulfuric acid.
In a preferred embodiment, the salt formation is carried out in a solvent
mixture of acetone/water in a ratio of between 5:1 and 4:1 by precipitation
using hydrochloric acid (37%). (3-Cyano-1H-indol-7-yl)[4-(4-fIuorophen-
ethyl)piperazin-1-yl]methanone, hydrochloride, is formed.
In a further embodiment of the process according to the invention, the
cyanoindole ester of the formula V can be reacted directly, without prior
saponification to 3-cyano-1H-indole-7-carboxylic acid, with 1-[2-(4-fluoro-
phenyl)ethyl]piperazine or a corresponding salt by chemical or biochemical
aminolysis.
The chemical aminolysis can be carried out, for example, by the method of
Menger F. M. et al., J. Am. Chem. Soc. 1969, 91, 5346-9.
The biochemical aminolysis can be carried out, for example, by reaction of
a compound of the formula V with 1-[2-(4-fluorophenyl)ethyl]piperazine or
one of the corresponding salts in the presence of a lipase or an antibody.
The biochemical aminolysis can be carried out, for example, by the method
of Gotor V. et al., Bioorg. Med. Chem. 1999, 7, 2189-2197.
The invention therefore likewise relates to a process for the preparation of
(3-cyano-1 H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1 -yl]methanone of
the formula I
and salts thereof, characterised in that
(1) an indole ester of the formula II
in which
R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
isformylated,
(2) the formyl ester of the formula III
in which R is as defined above,
formed from (1) is reacted with hydroxylamine to give an oxime derivative
of the formula IV
in which R is as defined above,
(3) the oxime of the formula IV is converted into a cyanoindole ester of the
formula V
in which R has one of the meanings indicated above,
(4) the ester of the formula V is converted into the compound of the formula
I by aminolysis using 1-[2-(4-fluorophenyl)ethyl]piperazine or one of the
salts, and
(5) the resultant base of the formula I is converted into one of its salts by
treatment with an acid.
In a further embodiment of the process according to the invention, the
compound 3-cyano-1 H-indole-7-carboxylic acid can be prepared by
halogenation of an indole ester of the formula II
as described above to give a compound of the formula VI
in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl
and
Hal is CI, Br or l,
followed by cyanation.
The halogenation of the compounds of the formula II, as described above,
is carried out under standard conditions (lit.: Houben-Weyl, Methoden der
Organ. Chemie [Methods of Organic Chemistry], Georg-Thieme-Verlag,
Stuttgart) or analogously to Heterocycles, 1986, 24. 2879-85, ibid. 1989,
29,1663-7; J. Am. Chem. Soc. 1985, 107, 2943-5; J. Org. Chem. 1993, 58,
2058-60, or J. Chem. Soc, Perkin Trans. 1, 1989, 2009-15.
The bromination or iodination in the 3-position of the indole can likewise be
carried out analogously to Bocchi et al. Synthesis 1982, 1096-1097.
Examples of suitable solvents for the halogenation are hydrocarbons, such
as benzene, toluene and xylene; halogenated hydrocarbons, such as
dichloromethane and chloroform; ketones, such as acetone and butanone;
alcohols, such as methanol, ethanol, isopropanol and n-butanol; ethers,
such as tetrahydrofuran (THF) and dioxane; amides, such as dimethyl-
formamide (DMF) and N-methylpyrrolidone; nitriles, such as acetonitrile,
and if desired mixtures of these solvents with one another.
Compounds prepared in accordance with the invention by halogenation
are, for example, methyl 3-chloro-1H-indole-7-carboxylate, methyl 3-bromo-
1H-indole-7-carboxylate, methyl 3-iodo-1H-indole-7-carboxylate, ethyl 3-
chloro-1H-indole-7-carboxylate, ethyl 3-bromo-1H-indole-7-carboxylate,
ethyl 3-iodo-1H-indole-7-carboxylate, tert-butyl 3-chloro-1H-indo!e-7-
carboxylate, tert-butyl 3-bromo-1H-indole-7-carboxylate, tert-butyl 3-iodo-
1H-indole-7-carboxylate, benzyl 3-chloro-1H-indole-7-carboxylate, benzyl 3-
bromo-1H-indole-7-carboxylate and benzyl 3-iodo-1H-indole-7-carboxylate.
The use of ethyl 3-bromo-1H-indole-7-carboxylate or ethyl 3-iodo-1H-
indole-7-carboxylate is particularly suitable in accordance with the
invention.
The substitution of the halogen group of the compounds of the formula VI
by the cyano group is carried out analogously to the method of Cassar L. et
al., Adv. Chem. Ser. 1974, 132, 252-73, with nickel catalysis or analogously
to the method of Sakamoto T. et al, J. Chem. Soc, Perkin Trans. 1 1999,
16, 2323-2326, or Chatani N. et al, J. Org. Chem. 1986, 51, 4714-16, with
palladium catalysis.
The cyano group is particularly preferably introduced with palladium cataly-
sis.
The invention therefore likewise relates to a process for the preparation of
(3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1 -yl]methanone of
the formula I
and salts thereof, characterised in that
(1) an indole ester of the formula II
in which
R is an alky! group having from 1 to 6 carbon atoms or arylalkyl,
is halogenated,
(2) the halogen group of the ester of the formula VI
in which R and Hal are as defined above,
formed from (1) is converted into a cyano group, with the ester being
saponified to 3-cyano-1H-indole-7-carboxylic acid at the same time,
(3) the 3-cyano-1H-indole-7-carboxylic acid is reacted with 1-[2-(4-fluoro-
phenyl)ethyl]piperazine or salts thereof to give the compound of the
formula I, and
(4) the resultant base of the formula I is converted into one of its salts by
treatment with an acid.
A further aspect of the invention relates to compounds of the formula IV
in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
and salts thereof.
Alkyl and arylalkyl have one of the meanings indicated above.
Compounds of the formula IV may occur in two isomeric forms, compounds
of the formulae IVa and IVb. The general formula IV covers the individual
isomers of the formulae IVa and IVb as well as mixtures thereof.
in which R has one of the meanings indicated above.
Preferred compounds of the formula IV are
methyl 3-hydroxyimino-1H-indole-7-carboxylate,
ethyl 3-hydroxyimino-1H-indole-7-carboxylate,
tert-butyl 3-hydroxyimino-1H-indole-7-carboxylate and
benzyl 3-hydroxyimino-1H-indole-7-carboxylate, where both the Z- and E-
forms and mixtures of these are included.
Particularly preferred compounds of the formula IV are
ethyl (2)-3-hydroxyimino-1H-indole-7-carboxylate,
ethyl (E)-3-hydroxyimino-1H-indole-7-carboxylate and E/Z mixtures.
A salt of the compounds of the formula IV can be prepared by the methods
described above for compounds of the formula I.
The compounds of the formula IV are valuable intermediates in the synthe-
sis of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]metha-
none and salts thereof, as described above.
A further aspect of the invention relates to compounds of the formula V
in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
and salts thereof.
Alkyl and arylalkyl have one of the meanings indicated above.
Preferred compounds of the formula V are
methyl 3-cyano-1H-indole-7-carboxylate,
ethyl 3-cyano-1H-indole-7-carboxylate,
tert-butyl 3-cyano-1H-indole-7-carboxylate and
benzyl 3-cyano-1H-indole-7-carboxylate, and salts thereof.
The process according to the invention is particularly preferably carried out
using ethyl 3-cyano-1H-indole-7-carboxylate.
A salt of the compounds of the formula V can be prepared by the methods
described above for compounds of the formula I.
The compounds of the formula V are valuable intermediates in the synthe-
sis of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]metha-
none and salts thereof, as described above.
The invention likewise relates to the compound 3-cyano-1H-indole-7-car-
boxylic acid and salts thereof.
A further aspect of the invention relates to compounds of the formula VI
in which R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
and
Hal is Cl, Br or I
and salts thereof.
Alkyl and arylalkyl have one of the meanings indicated above.
Preferred compounds of the formula VI are
methyl 3-bromo-1H-indole-7-carboxylate,
ethyl 3-bromo-1H-indole-7-carboxylate,
tert-butyl 3-bromo-1H-indole-7-carboxylate and
benzyl 3-bromo-1H-indole-7-carboxylate,
methyl 3-iodo-1H-indole-7-carboxylate,
ethyl 3-iodo-1H-indole-7-carboxylate,
tert-butyl 3-iodo-1H-indole-7-carboxylate and
benzyl 3-iodo-1H-indole-7-carboxylate, and salts thereof.
The process according to the invention is particularly preferably carried out
using ethyl 3-bromo-1H-indole-7-carboxylate or ethyl 3-iodo-1H-indole-7-
carboxylate.
A salt of the compounds of the formula VI can be prepared by the methods
described above for compounds of the formula I.
The compounds of the formula VI are valuable intermediates in the synthe-
sis of (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl]metha-
none and salts thereof, as described above.
Even without further details, it is assumed that a person skilled in the art
will be able to utilise the above description in the broadest scope. The
preferred embodiments should therefore merely be regarded as descriptive
disclosure which is absolutely not to be regarded as limiting in any way.
All temperature data above and below are given in °C. In the following
examples, "conventional work-up" means that water is added if necessary,
the mixture is adjusted, if necessary, to a pH of between 2 and 10,
depending on the constitution of the end product, the mixture is extracted
with ethyl acetate or dichloromethane, the phases are separated, the
organic phase is dried over sodium sulfate and evaporated, and the
product is purified by chromatography on silica gel and/or by crystallisation.
Example 1:
1.1. Methyl 3-formylindole-7-carboxylate
2.9 ml of phosphorus oxychloride are added slowly to 7 ml of N,N-dimethyl-
formamide in a nitrogen atmosphere (formylation solution). 5 g (0.029 mol)
of methyl indole-7-carboxy!ate are dissolved in 7 ml of DMF and added
slowly to the formylation solution, during which the temperature does not
rise above 30 degrees. The mixture is then warmed at 100° for one hour.
After cooling, the mixture is poured into water and neutralised using sodium
hydroxide solution, and the deposited crystals are filtered off with suction,
m.p. 154°. Yield 5.3 g (89.9% of theory).
1.2. Methyl 3-(hydroxyiminomethyl)indole-7-carboxylate
5 g of methyl 3-formylindole-7-carboxylate (0.024 mol) are added to a solu-
tion of 0.03 mol of hydroxylammonium hydrochloride in dimethylformamide.
The reaction mixture is heated at 125° for one hour and subjected to con-
ventional work-up, giving 5.1 g of methyl 3-(hydroxyiminomethyl)indole-7-
carboxylate.
1.3. Methyl 3-cyanoindole-7-carboxylate
5 g of methyl 3-(hydroxyiminomethyl)indole-7-carboxylate are suspended in
20 ml of toluene, 1 ml of sulfonyl chloride is added, and the mixture is
refluxed for one hour. Evaporation and extraction with ethyl acetate give
4.5 g of methyl cyanoindole-7-carboxylate, m.p. 212°.
1.4. 3-Cyanoindole-7-carboxylic acid
4.5 g (0.022 mol) of methyl cyanoindole-7-carboxylate are suspended in
100 ml of methanol, and a solution of 30 ml of sodium hydroxide solution
(w = 32%) in 30 ml of water is added at room temperature. Stirring over-
night gives a virtually clear solution, which is filtered and evaporated. Water
is added to the residue until a clear solution is formed, and the mixture is
adjusted to pH = 2 using concentrated hydrochloric acid with ice-cooling.
The white crystals are filtered off with suction and dried for 2 hours under
reduced pressure, giving 4 g of 3-cyanoindole-7-carboxylic acid (97.7% of
theory); m.p. 317.5 - 318.5°.
1.5. 7-{4-[2-(4-FIuorophenyl)ethyl]piperazine-1 -carbonyl}-1H-indole-
3-carbonitrile
5 g (0.027 mol) of 3-cyanoindole-7-carboxylic acid are dissolved in 40 ml of
hot N-methylpyrrolidone, the solution is cooled to 40°, and 7.6 g
(0.027 mol) of N,N-carbonyldiimidazole are added. The mixture is stirred at
room temperature for a further one hour. A suspension of 1-(2-(4-fluoro-
phenyl)ethyl)piperazine dihydrochloride in 40 ml of N-methylpyrrolidone is
subsequently poured in. After 5 minutes, a clear solution is formed and just
afterwards white crystals deposit. The mixture is stirred overnight at room
temperature. The crystals are filtered off with suction, washed and dried,
giving 5 g of 7-{4-[2-(4-fluorophenyl)ethyl]piperazin-1-carbonyl}-1H-indole-
3-carbonitrile as the free base having a melting point (m.p.) of 192.0 -
193.5°.
The chemical names 7-{4-[2-(4-fluorophenyl)ethyl]piperazine-1-carbonyl}-
1H-indole-3-carbonitrile and (3-cyano-1H-indol-7-yl)[4-(4-fluorophenethyl)-
piperazin-1-yl]methanone are synonymous.
1.6. 7-{4-[2-(4-Fluorophenyl)ethyl]piperazin-1 -carbonyl}-1H-indole-
3-carbonitrile, hydrochloride
2.1g of the free base obtained in accordance with 1.5 are heated in 50 ml
of acetone, and water is added until a clear solution is formed. A mixture of
0.6 ml of hydrochloric acid (w = 37%) and 1.2 ml of acetone is then stirred
in. The mixture is subsequently evaporated to half the volume in a rotary
evaporator. The precipitated hydrochloride is filtered off with suction,
washed with acetone and diethyl ether and dried, giving 1.6 g of 7-{4-[2-(4-
fluorophenyl)ethyl]piperazin-1-carbonyl}-1H-indole-3-carbonitrile, hydro-
chloride (69% of theory), decomposition range 314 - 319°.
Example 2:
2.1. Methyl 3-cyanoindole-7-carboxylate
9.1 g of phosphoryl chloride are added to 30 ml of dimethylformamide with
ice cooling at a reaction temperature of 20-30°. A solution of 8 g of methyl
indole-7-carboxylate in dimethylformamide is added dropwise, during which
the temperature rises to 40°. After one hour at 125°, the solution is added
dropwise while still hot to a solution of 6.3 g of hydroxylammonium chloride
in 40 ml of dimethylformamide, and the mixture is stirred at 120°C for a fur-
ther 15 minutes. The mixture is poured into water, extracted with ethyl ace-
tate, filtered through neutral aluminium oxide and evaporated, giving 4.5 g
of methyl 3-cyanoindote-7-carboxylate having a melting range of 212 -
213.5° (48.9% of theory).
The further reaction is carried out analogously to Example 1.4. to 1.6.
Example 3:
3.1. Ethyl 3-bromoindole-7-carboxylate
12 g of pyridine hydrobromide perbromide are added to a solution of 5 g of
ethyl indole-7-carboxylate in 50 g of pyridine. The reaction mixture is
warmed to 30-50° and stirred until the conversion is complete (from about 3
to 10 hours).
Conventional work-up gives ethyl 3-bromoindole-7-carboxylate.
3.2. 3-Cyanoindole-7-carboxylic acid
7 g of ethyl 3-bromoindole-7-carboxylate are dissolved in 70 g of NMP, and
4 g of CuCN are added. The mixture is heated to 100-140°C with stirring.
After 3 hours, the mixture is subjected to conventional work-up, giving
3-cyanoindole-7-carboxylic acid.
The further reaction of the 3-cyanoindole-7-carboxylic acid is carried out
analogously to Example 1.5. to 1.6.
WE CLAIM :
1. Process for the preparation of (3-cyano-1H-indol-7-yl)[4-(4-fluorophen-
ethyl)piperazin-1-yl]methanone of the formula I
and salts thereof, characterised in that
(1) an indole ester of the formula II
in which
R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
is formylated,
(2) the formyl ester of the formula III
in which R is as defined above,
formed from (1) is reacted with hydroxylamine to give an oxime deriva-
tive of the formula IV
in which R is as defined above,
(3) the oxime of the formula IV is converted into a cyanoindole ester of
the formula V
in which R has one of the meanings indicated above,
(4) the ester of the formula V is saponified to give 3-cyano-1H-indole-7-
carboxylic acid,
(5) the 3-cyano-1H-indole-7-carboxylic acid is reacted with 1-[2-(4-
fluorophenyl)ethyl]piperazine or salts thereof to give the compound of
the formula I, and
(6) the resultant base of the formula I is converted into one of its salts
by treatment with an acid.
Process as claimed in Claim 1 wherein steps (1) to (3) are
carried out as a one-pot synthesis.
3. Process as claimed in Claim 1 where in step (4), the
ester of the formula V is converted into the compound of the formula I
by aminolysis using 1-[2-(4-fluorophenyl)ethyl]piperazine or one of the
salts.
4. Process as claimed in Claim 1 wherein 3-cyano-1H-indole-
7-carboxylic acid is prepared by halogenation of an indole ester of the
formula II
in accordance with Claim 1 to give a compound of the formula VI
in which R is an alkyl group having from 1 to 6 carbon atoms or aryl-
alkyl, and
Hal is CI, Br or l,
followed by cyanation.
5. Process as claimed in one or more of Claims 1 to 4, wherein
R is ethyl.
6. Process as claimed in one or more of Claims 1 to 5, wherein
that 1-[2-(4-fluorophenyl)ethyl]piperazine is employed as the dihydro-
chloride.
7. Process as claimed in one or more of Claims 1 to 6, wherein
that the base (3-cyano-1H-indoI-7-yl)[4-(4-fluorophenethyl)piperazin-1 -
yl]methanone is converted into its hydrochloride.
8. Oxime derivatives of the formula IV
in which
R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and
salts thereof.
9. Cyanoindole esters of the formula V
in which
R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl,
and salts thereof.
10. 3-Cyano-1H-indole-7-carboxylic acid and salts thereof.
11. Compounds of the formula VI
in which
R is an alkyl group having from 1 to 6 carbon atoms or arylalkyl, and
Hal is Cl, Br or I,
and salts thereof.
The present Invention relates to a process for the preparation of
(3-cyano-lH-Indol-7-yl)[4-(4-fluorophenethyl)piperazin-1-yl)]
methanone and salts thereof, characterized in that an Indole ester
of the formula II in which R is as defined in claim1, is converted
into 3-cyano-1H-indole-7-carboxylic acid via steps (1) to (4)
according to Claim 1, and this Is reacted with 1-[2-(4-
fluorophenyl)ethyl]piperazine of salts thereof to give the product.

Documents:

1046-kolnp-2003-granted-abstract.pdf

1046-kolnp-2003-granted-claims.pdf

1046-kolnp-2003-granted-correspondence.pdf

1046-kolnp-2003-granted-description (complete).pdf

1046-kolnp-2003-granted-examination report.pdf

1046-kolnp-2003-granted-form 1.pdf

1046-kolnp-2003-granted-form 18.pdf

1046-kolnp-2003-granted-form 2.pdf

1046-kolnp-2003-granted-form 3.pdf

1046-kolnp-2003-granted-form 5.pdf

1046-kolnp-2003-granted-gpa.pdf

1046-kolnp-2003-granted-letter patent.pdf

1046-kolnp-2003-granted-reply to examination report.pdf

1046-kolnp-2003-granted-specification.pdf

1046-kolnp-2003-granted-translated copy of priority document.pdf

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Patent Number

216050

Indian Patent Application Number

01046/KOLNP/2003

PG Journal Number

10/2008

Publication Date

07-Mar-2008

Grant Date

06-Mar-2008

Date of Filing

18-Aug-2003

Name of Patentee

MERCK PATENT GMBH.

Applicant Address

FRANKFURTER STRASSE 250, 64293 DARMSTADT

Inventors:

#	Inventor's Name	Inventor's Address
1	CRASSIER, HELENE	SANDSTRASSE 30, 64331 WEITERSTADT, DE
2	ECKERT, UWE	HEIDELBERGER STRASSE 51a, 64285 DARMSTADT, DE
3	BOTTCHER, HENNING	STIFTSTRASSE 12, 64287 DARMSTADT, DE
4	BATHE, ANDREAS	MERCKSTRASSE 17, 64283 DARMSTADT, DE
5	EMMERT, STEFFEN	MITTELSTRASSE 41, 64331 WEITERSTADT, DE

PCT International Classification Number

C 07 D 209/14

PCT International Application Number

PCT/EP01/15240

PCT International Filing date

2001-12-21

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	101 02 944.6	2001-01-23	Germany