Title of Invention

SULFONAMIDO ETHER SUBSTITUTED IMIDAZOQUINOLINES

Abstract Imidazopyridine compounds that contain substituted amine functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.
Full Text

Sulfonamido Ether Substituted Imidazoquinolines
Field of the Invention
This invention relates to imidazoquinoline compounds that have ether and sulfonamide or sulfamide functionality at the 1-position, and to pharmaceutical compositions containing such compounds. A further aspect of this invention relates to the use of these compounds as immunomodulators, for inducing cytokine biosynthesis in animals, and in the treatment of diseases, including viral and neoplastic diseases.
Background of the Invention
The first reliable report on the lH-imidazo[4,5-c]quinoline ring system, Backman et al., J. Org. Chem. 15, 1278-1284 (1950) describes the synthesis of l-(6-methoxy-8' quinolinyl)-2-methyl-lH-imidazo[4,5-c]quinoline for possible use as an antimalarial agent. Subsequently, syntheses of various substituted lH-imidazo[4,5-c] quinolines were reported. For example. Jain ct a!., J. Med. Chem. 11, pp. 87-92 (1968), synthesized the compound l-[2-(4-piperidyl)ethyl]-lH-imidazo[4,5-c]quinoline as a possible anticonvulsant and cardiovascular agent. Also, Baranov et al., Chem. Abs. 85, 94362 (1976), have reported several 2-oxoimidazo[4,5-c]quinolines, and Berenyi et al., L Heterocyclic Chem. 18, 1537-1540 (1981), have reported certain 2-oxoimidazo[4,5-cjquinolines.
Certain lH-imidazo[4,5-c]quinolin-4-amines and 1- and 2-substituted derivatives thereof were later found to be useful as antiviral agents, bronchodilators and immunomodulators. These are described in, inter alia, U.S. Patent Nos. 4,689,338; 4,698,348; 4,929,624; 5,037,986; 5,268,376; 5,346,905; and 5,389,640, all of which are incorporated herein by reference.
There continues to be interest in the imidazoquinoline ring system. Certain IH-imidazo[4,5-c] naphthyridine'4-amines, IH-imidazo [4,5-c] pyridin-4-amines, and IH-imidazo[4,5-c] quinolin-4-amines having an ether containing substituent at the 1 position are known. These are described in U.S. Patent Nos. 5,268,376; 5,389,640; 5,494,916; and WO 99/29693.
There is a continuing need for compounds that have the ability to modulate the immune response, by induction of cytokine biosynthesis or other mechanisms.

Summary of the Invention
We have found a new class of compounds that are useful in inducing cytokine biosynthesis in animals. Accordingly, this invention provides imidazoquinoIine-4-amine and tetrahydroimidazoquinoline-4-amine compounds that have an ether and sulfonamide or sulfamide containing substituent at the 1-position. The compounds are defined by Formulas (I) and (II), which are defined in more detail infra. These compounds share the general structural formula

wherein X, R1, R2, and R are as defined herein for each class of compounds having Formulas (I) and (II),
The compounds of Formulas (I) and (II) are useful as immune response modifiers due to their ability to induce cytokine biosynthesis and otherwise modulate the immune response when administered to animals. This makes the compounds useful in the treatment of a variety of conditions such as viral diseases and tumors that are responsive to such changes in the immune response.
The invention further provides pharmaceutical compositions containing the immune response modifying compounds, and methods of inducing cytokine biosynthesis in an animal, treating a viral infection in an animal* and/or treating a neoplastic disease in an animal by administering a compound of Formula (I) or (II) to the animal.
In addition, the invention provides methods of synthesizing the compounds of the invention and novel intermediates useful in the synthesis of these compounds.

Detailed Description of the Invention
As mentioned earlier, we have found certain compounds that induce cytokine biosynthesis and modify the immune response in animals. Such compounds are represented by Formulas (I) and (II), shown below.
Imidazoquinoline compounds of the invention, which have ether and sulfonamide or sulfamide functionality at the 1-position are represented by Formula (I):

wherein: X is -CHR5-, -CHRs-alkyl-, or -CHRs-alkenyl-;
R1 is selected from the group consisting of: -R4-NR3-S02~R6-alkyl; -R4-NR3-S02-R6-alkenyl; -R4-NR3-S02-R6-aryl; -R4~NR3-S02-R6-heteroary 1; -R4-NR3-S02-R6-heterocyclyl;
-R4-NR3-SO2-R7;
-R4-NR3-S02-NR5-R6-alkyl;
-R4-NR3-S02-NRs-R6-alkenyl;
-R4-NR3-S02-NR5-R6-aryl;
-R4-NR3 -S02-NRs-R6-heteroary 1;
-R4-NR3-S02-NR5-R«-heterocyclyl;and
-R4-NR3-SO2-NH2; R2 is selected from the group consisting of: -hydrogen;

-alkyl;
-alkenyl;
-aryl;
heteroaryl;
-heterocyclyl;
-alkyl-Y-alkyl;
-alkyl-Y-alkenyl;
-alkyl-Y-aryl; and
- alkyl or alkenyl substituted by one or more substituents selected
from the group consisting of:
-OH;
-halogen;
-N(R5)2; -CO-N(R5)2; -CO-C1-10 alkyl; -CO-O-C1-10 alkyl;
-N3;
-aryl;
-heteroaryl;
-heterocyclyl;
-CO-aryl; and
-CO-heteroaryl;
Y is-0-or-S(0)o-2S
R3 is H, C1-10 alkyl, or alylalkyl;
each R4 is independently alkyl or alkenyl, which may be interrupted by one
or more -O- groups; or R3 and R4 can join together to form a ring;
each R5 is independently H, C1-10alkyl, or C2-10 alkenyl;
R6 is is a bond, alkyl, or alkenyl, which may be interrupted by one or more
-O- groups;
R7 is CMO alkyl; or R3 and R7 can join together to form a ring;
n is 0 to 4; and

each R present is independently selected from the group consisting of CMO alkyl, C1-10 alkoxy, hydroxy, halogen and trifluoromethyl; or a pharmaceutically acceptable salt thereof. The invention also includes tetrahydroimidazoquinoline compounds that bear an ether and sulfanomide or sulfamide containing substituent at the 1-position. Such tetrahydroimidazoquinoline compounds are represented by Formula (II);

wherein: X is -CHR5-, -CHRj-alkyl-, or -CHRs-alkenyl-;
Ri is selected from the group consisting of: -R4-NR3-S02-R6-alkyl; -R4-NR3-SO2-R6—alkenyl; -R4-NR3-S02-R6-aryl; -R4-NR3-S02-R6-heteroaryl; -R4-NR3-S02-R6-heterocyclyl;
-R4-NR3-SO2-R7; -R4-NR3-S02-NR5-R6-alkyl; -R4-NR3-S02-NR5-R6-alkenyl; -R4-NR3-S02-NR5-R6-aryl; -R4-NR3-S02-NRrR6-heteroaryl; -R4-NR3-S02-NR5-R6-heterocyclyl;and -R4-NR3-SO2-NH2; R2 is selected from the group consisting of: -hydrogen; -alkyl;

-alkenyl;
-aryl;
-heteroaryl;
-heterocyclyl;
-alkyl-Y-alkyl;
-alkyl'Y-alkenyl;
-alkyl-Y-aryl; and
- alkyl or alkenyl substituted by one or more substituents selected
from the group consisting of:
-OH;
-halogen;
-N(R5)2; -CO-N(R5)2; -CO-C1-10 alkyl;
-CO-O-C1-10 alkyl;
-N3;
-aryl;
-heteroaryl;
-heterocyclyl;
-CO-aryl; and
-CO-heteroaryl;
Y is -0- or -S(0)o-2-;
R3 is H, CMO alkyl, or arylalkyl;
each R4 is independently alkyl or alkenyl, which may be interrupted by one
or more -O- groups; or R3 and R4 can join together to form a ring;
each R5 is independently H, C1-10 alkyl, or C2.10 alkenyl;
R6 is a bond, alkyl, or alkenyl, which may be interrupted by one or more
-O- groups;
R7 is C1-10 alkyl; or R3 and R7 can join together to form a ring;
n is 0 to 4; and

each R present is independently selected from the group consisting of Ci,io alkyl, C1-10 alkoxy, hydroxy, halogen, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
Preparation of the Compounds
Compounds of the invention can be prepared according to Reaction Scheme I where R, R1, R2, X and n are as defined above.
In step (1) of Reaction Scheme I a 2,4-dichloro-3-nitroquinoline of Formula X is reacted with an amine of Formula R1-O-X-NH2 to provide a 2-chloro-3-nitroquinolin-4-amine of Formula XI. The reaction can be carried out by adding the amine to a solution of a compound of Formula X in a suitable solvent such as chloroform or dichloromethane and optionally heating. Many quinolines of Formula XI are known or can be prepared using known synthetic methods, see for example, Andre et al., U,S. Patent No. 4,988,815 and references cited therein. Many amines of Formula R1-0'X-NH2 are known; some are commercially available and others can by prepared using known synthetic methods.
In step (2) of Reaction Scheme I a 2-chloro-3-nitroquinolin-4-amine of Formula XI is reduced to provide a 2-chloroquinoline'3,4-diamine of Formula XII. Preferably, the reduction is carried out using a conventional heterogeneous hydrogenation catalyst such as platinum on carbon or palladium on carbon. The reaction can conveniently be carried out on a Parr apparatus in a suitable solvent such as ethanol, isopropanol or toluene.
In step (3) of Reaction Scheme I a 2'Chloroquinoline-3,4-diamine of Formula XII is reacted with a carboxylic acid or an equivalent thereof to provide a 4-chloxo-lH-imidazo[4,5-c]quinoline of Formula XIII. Suitable equivalents to carboxylic acid include orthoesters and 1,1-diaIkoxyalkyl alkanoates. The carboxylic acid or equivalent is selected such that it will provide the desired R2 substituent in a compound of Formula XIIL For example, triethyl orthoformate will provide a compound where R2 is hydrogen and triethyl orthoacetate will provide a compound where R2 is methyl. The reaction can be run in the absence of solvent or in an inert solvent such as toluene. The reaction is run with sufficient heating to drive off any alcohol or water formed as a byproduct of the reaction.
Alternatively, step (3) can be carried out by (i) reacting the diamine of Formula XII with an acyl halide of Formula R2C(0)C1 and then (ii) cyclizing. In part (i) the acyl halide

is added to a solution of the diamine in an inert solvent such as acetonitrile or dichloromethane. The reaction can be carried out at ambient temperature. The product can be isolated using conventional methods. In part (ii) the product of part (i) is heated in an alcoholic solvent in the presence of a base. Preferably the product of part (i) is refluxed in ethanol in the presence of an excess of triethylamine or heated with methanolic ammonia.
In step (4) of Reaction Scheme I a 4-chloro-lH-imidazo[4,5-c]quinoline of Formula XIII is aminated to provide a lH-imidazo[4,5-c]quinolin-4-amine of Formula I. The reaction is carried out by heating (e.g., 125-175°C) a compound of Formula XIII under pressure in a sealed reactor in the presence of a solution of ammonia in an alkanol. The product or a phannaceutically acceptable salt thereof can be isolated using conventional methods.


is tert-butoxycarbonyI and R11 is -R6-alkyl, -R6-aryl, -R6-heteroaryl or -R6-heterocyclyl where R6 is as defined above.
In step (1) of Reaction Scheme II the amino group of an aminoalcohol of Formula XIV is protected with a tert-butoxycarbonyl group. A solution of the aminoalcohol in tetrahydrofuran is treated with di-tert-butyl dicarbonate in the presence of a base such as sodium hydroxide. Many aminoalcohols of Formula XIV are commercially available; others can be prepared using known synthetic methods.
In step (2) of Reaction Scheme II a protected aminoalcohol of Formula XV is converted to an iodide of Formula XVI. Iodine is added to a solution of triphenylphosphine and imidazole in dichloromethane; then a solution of a protected aminoalcohol of Formula XV in dichloromethane is added. The reaction is carried out at ambient temperature.
In step (3) of Reaction Scheme II a lH-imidazo[4,5-c]quinolin*l-yl alcohol of Formula XVII is alkylated with an iodide of Formula XVI to provide a lH-imidazo[4,5-c]quinolin-l-yl ether of Formula XVIII. The alcohol of Formula XVII is reacted with sodium hydride in a suitable solvent such as N,N-dimethylformamide to form an alkoxide. The iodide is added to the alkoxide solution at ambient temperature. After the addition is complete the reaction is stirred at an elevated temperature (-lOO°C). Many compounds of Formula XVII are known, see for example, Gerster, U.S. Patent 4,689,338; others can readily be prepared using known synthetic routes, see for example, Gerster et al., U.S. Patent No. 5.605,899 and Gerster, U.S. Patent No. 5,175,296,
In step (4) of Reaction Scheme II a lH--imidazo[4,5-c]quinolin-l-yl ether of Formula XVIII is oxidized to provide a lH-imidazo[4,5-c]quinoline-5N-oxide of Formula XIX using a conventional oxidizing agent capable of forming N-oxides. Preferably a solution of a compound of Formula XVIII in chloroform is oxidized using 3-chloroperoxybenzoic acid at ambient temperature.
In step (5) of Reaction Scheme II a lH-imidazo[4,5-c]quinoline-5N-oxide of Formula XIX is aminated to provide a lH-imidazo[4,5-c]quinolin-4-amine of Formula XX. Step (5) involves (i) reacting a compound of Formula XIX with an acylating agent and then (ii) reacting the product with an aminating agent. Part (i) of step (5) involves reacting an N-oxide of Formula XIX with an acylating agent. Suitable acylating agents include alkyl- or arylsulfonyl chlorides (e.g., benezenesulfonyl chloride, methanesulfonyl

chloride, p-toluenesulfonyl chloride), Arylsulfonyl chlorides are preferred. Para-toluenesulfonyl chloride is most preferred. Part (ii) of step (5) involves reacting the product of part (i) with an excess of an aminating agent. Suitable aminating agents include ammonia (e.g., in the form of ammonium hydroxide) and ammonium salts (e.g., ammonium carbonate, ammonium bicarbonate, ammonium phosphate). Ammonium hydroxide is preferred. The reaction is preferably carried out by dissolving the N-oxide of Formula XIX in an inert solvent such as dichloromethane or 1,2-dichloroethane with heating if necessary, adding the aminating agent to the solution, and then slowly adding the acylating agent. Optionally the reaction can be carried out in a sealed pressure vessel at an elevated temperature (85-100°-),
In step (6) of Reaction Scheme II the protecting group is removed by hydrolysis under acidic conditions to provide a lH-imidazo[4,5-c]quinolin-4-amine of Formula XXI. Preferably the compound of Formula XX is treated with hydrochloric acid/ethanol at ambient temperature or with gentle heating.
In step (7) of Reaction Scheme II a IHA-imidazo[4,5-c]quinolin'4-amine of Fonnula XXI is converted to a sulfonamide of Formula XXII which is a subgenus of Formula I using conventional synthetic methods. For example, a compound of Formula XXI can be reacted with a sulfonyl chloride of Formula RnS(02)Cl. The reaction can be carried out by adding a solution of the sulfonyl chloride in a suitable solvent such as dichloromethane or 1-methyl-2-pyrrolidinone to a solution of a compound of Formula XXI at ambient temperature. Alternatively, a compound of Formula XXI can be reacted with a sulfonic anhydride of Formula RuS(02)OS(02)R11. The reaction can be run at ambient temperature in an inert solvent such as dichloromethane in the presence of a base such as pyridine or N,N-diisopropylethylamine, The product or a pharmaceutically acceptable salt thereof can be isolated using conventional methods.


Compounds of the invention containing a sulfonamide group can be prepared according to Reaction Scheme III where R, R2, R3, R4, R11, X and n are as defined above and BOC is tert-butoxycarbonyl.
In step (1) of Reaction Scheme III the amino group of an aminoalcohol of Formula XXIII is protected with a tert-butoxycarbonyl group. A solution of the aminoalcohol in tetrahydrofuran is treated with di-/e/t-butyl dicarbonate in the presence of a base such as sodium hydroxide. Many aminoalcohols of Formula XXIII are commercially available; others can be prepared using known synthetic methods.

In step (2) of Reaction Scheme III a protected amino alcohol of Fonnula XXIV is converted to a methanesulfonate of Formula XXV. A solution of a compound of Formula XXIV in a suitable solvent such as dichloromethane is treated with methanesulfonyl chloride in the presence of a base such as triethylamine. The reaction can be carried out at a reduced temperature (0°C),
In step (3a) of Reaction Scheme III a methanesulfonate of Formula XXV is converted to an azide of Formula XXVI. Sodium azide is added to a solution of a compound of Formula XXV in a suitable solvent such as N,N-dimethylformamide or tctrahydrofuran. The reaction can be carried out at an elevated temperature (80 - 100'*C).
In step (3b) of Reaction Scheme III a compound of Formula XXVI is alkylated with a halide of Formula Hal-R3 to provide a compound of Formula XXVII. In compounds where R3 is hydrogen this step is omitted. The compound of Formula XXVI is reacted with sodium hydride in a suitable solvent such as N,N-dimethylformamide to form the anion and then combined with the halide. The reaction can be carried out at ambient temperature.
In step (4) of Reaction Scheme III an azide of Formula XXVI or XXVII is reduced to provide an amine of Formula XXVIIL Preferably, the reduction is carried out using a conventional heterogeneous hydrogenation catalyst such as palladium on carbon. The reaction can conveniently be carried out on a Parr apparatus in a suitable solvent such as methanol or isopropanol.
In step (5) of Reaction Scheme III a 4-chloro-3-nitroquinoline of Formula XXIX is reacted with an amine of Formula XXVIII to provide a 3-nitroquinoline of Formula XXX. The reaction can be carried out by adding an amine of Formula XXVIII to a solution of a compound of Formula XXIX in a suitable solvent such as dichloromethane in the presence of a base such as triethylamine. Many quinolines of Fonnula XXIX are known compounds or can be prepared using known synthetic methods, see for example, U.S. Patent 4,689,338 and references cited therein.
In step (6) of Reaction Scheme III a 3-nitroquinoline of Formula XXX is reduced to provide a 3-aminoquinoline of Formula XXXI. Preferably, the reduction is carried out using a conventional heterogeneous hydrogenation catalyst such as platinum on carbon. The reaction can conveniently be carried out on a Pan* apparatus in a suitable solvent such as toluene.

In step (7) of Reaction Scheme III a compound of Formula XXXI is reacted with a carboxylic acid or an equivalent thereof to provide a lH-imidazo[4,5-c]quinoline of Formula XVIII. Suitable equivalents to carboxylic acid include orthoesters, and 1,1-dialkoxyalkyl alkanoates. The carboxylic acid or equivalent is selected such that it will provide the desired R2 substituent in a compound of Formula XVIII. For example, triethyl orthoformate will provide a compound where R2 is hydrogen and triethyl orthovalerate will provide a compound where R2 is butyl The reaction can be run in the absence of solvent or in an inert solvent such as toluene. The reaction is run with sufficient heating to drive off any alcohol or water formed as a byproduct of the reaction. Optionally a catalytic amount of pyridine hydrochloride can be included.
Alternatively, step (7) can be carried out by (i) reacting a compound of Formula XXXI with an acyl halide of Formula R2C(0)C1 and then (ii) cyclizing. In part (i) the acyl halide is added to a solution of a compound of Formula XXXI in an inert solvent such as acetonitrile or dichloromethane, The reaction can be carried out at ambient temperature or at a reduced temperature. In part (ii) the product of part (i) is heated in an alcoholic solvent in the presence of a base. Preferably the product of part (i) is refluxed in ethanol in the presence of an excess of triethylamine or heated with methanolic ammonia.
Steps (8), (9), (10) and (11) are carried out in the same manner as steps (4), (5), (6) and (7) of Reaction Scheme II.
Reaction Scheme III



Compounds of the invention containing a sulfamide group can be prepared according to Reaction Scheme IV where R, R2, R3, R4, R5, R11, X and n are as defined above.
In step (1) of Reaction Scheme IV a lH-imidazo[4,5-c?]quinolin- 4-amine of Formula XXI is reacted with sulfiiryl chloride to generate in situ a sulfamoyl chloride of Formula XXXII. The reaction can be carried out by adding a solution of sulfiiryl chloride in dichloromethane to a solution of a compound of Formula XXI in dichloromethane in the presence of 1 equivalent of 4-(dimethylamino)pyridine. The reaction is preferably carried out at a reduced temperature (-78°C).
In step (2) of Reaction Scheme IV an amine of Formula HNR5R1] is reacted with the sulfamoyl chloride of Formula XXXII to provide a sulfamide of Formula XXXIII which is a subgenus of Formula 1. The reaction can be carried out by adding a solution containing 2 equivalents of the amine and 2 equivalents of triethylamine in dichloromethane to the reaction mixture from step (1). The addition is preferably carried out at a reduced temperature (-78°C). After the addition is complete the reaction mixture can be allowed to warm to ambient temperature. The product or a pharmaceutically acceptable salt thereof can be isolated using conventional methods.


Compounds of the invention can be prepared according to Reaction Scheme V where R, R2, R3, R4, Ri 1, X and n are as defined above.
In step (1) of Reaction Scheme V a lH-imidazo[4,5-c]quinolin-4-amine of Formula XXI is reduced to provide a 6,7,8,9-tetrahydro-lH-imidazo[4,5-c]quinolin'4-amine of Formula XXXIV. Preferably the reduction is carried out by suspending or dissolving a compound of Formula XXI in trifluoroacetic acid, adding a catalytic amount of platinum (IV) oxide, and then hydrogenating. The reaction can be conveniently carried out in a Parr apparatus.
Step (2) is carried out in the same manner as step (7) of Reaction Scheme II to provide a 6,7,8,9-tetrahydro-lH-'imidazo[4,5-c]quinolin-4-amine of Formula XXXV which is a subgenus of Formula II. The product or a pharmaceutically acceptable salt thereof can be isolated using conventional methods.

Compounds of the invention containing a sulfamide group can be prepared according to Reaction Scheme VI where R, R2, R3, R4, R5, R11 X and n are as defined above.
In step (1) of Reaction Scheme VI a lH-imidazo[4,5-c]quinolin-4-amine of Formula XXXIV is reacted with sulfuryl chloride to generate in situ a sulfamoyl chloride of Formula XXXVI. The reaction can be carried out by adding a solution of sulfuryl chloride in dichloromethane to a solution of a compound of Formula XXXIV in dichloromethane in the presence of 1 equivalent of 4-(dimethylamino)pyridine. The reaction is preferably carried out at a reduced temperature (-78°C).

In step (2) of Reaction Scheme VI an amine of Formula HNR5R11 is reacted with the sulfamoyl chloride of Formula XXXVT to provide a sulfamide of Formula XXXVII which is a subgenus of Formula II. The reaction can be carried out by adding a solution containing 2 equivalents of the amine and 2 equivalents of triethylamine in dichloromethane to the reaction mixture from step (1). The addition is preferably carried out at a reduced temperature (-78°C), After the addition is complete the reaction mixture can be allowed to warm to ambient temperature. The product or a pharmaceutically acceptable salt thereof can be isolated using conventional methods.


wherein X is -CHR5-, -CHR5-alkyl-, or -CHR5-alkenyl-;
Ri is selected from the group consisting of: -R4-NR3-S02-R6-alkyl; -R4—NR3—SO2—R6-alkenyl; -R4-NR3-S02-R6-aryl; -R4-NR3-S02-R6-heteroaryl; -R4-NR3-S02-R6-heterocyclyl;
-R4-NR3-SO2-R7;
-R4-NR3-S02-NR5-R6-alkyl;
-R4-NR3-S02-NR5-R6-alkenyl;
-R4-NR3-S02-NR5-R6-aryl;
-R4-NR3-S02-NR5-R6-heteroaryl;
-R4-NfR3-S02-NR5-R6-heterocyclyl;and
-R4-NR3-SO2-NH2; R2 is selected from the group consisting of: -hydrogen; -alkyl; -alkenyl; -aryl;
-heteroaryl; -heterocyclyl;.. -alkyl-Y-alkyl; -alkyl-Y- alkenyl; -alkyl-Y-aryl; and
- alkyl or alkenyl substituted by one or more substituents selected from the group consisting of:
-OH;
-halogen;
-N(R5)2; -CO-N(R5)2; -CO-Ci-io alkyl;

-CO-O-C1-10alkyl;
-aryl;
-heteroaryl;
-heterocyclyl;
-CO-aryl; and
-CO-heteroaryl; Y is -0' or -S(0)o.2-; R3 is H, C1-1 alkyl, or arylalkyl;
each R4 is independently alkyl or alkenyl, which may be interrupted by one or more -O- groups; or R3 and R4 can join to form a ring; each R5 is independently H, C1-10 alkyl, or C2-10 alkenyl; R6 is a bond, or is alkyl or alkenyl, which may be interrupted by one or more -O- groups;
R7 is C1-10 alkyl; or R3 and R7 can join together to form a bond; n is 0 to 4; and
each R present is independently selected from the group consisting ofC1-10 alkyl, C1-10 alkoxy, hydroxy, halogen and trifluoromethyl; or a pharmaceutically acceptable salt thereof
As used herein, the terms -alkyl-, -alkenyl- and the prefix -alk-- are inclusive of
both straight chain and branched chain groups and of cyclic groups, i.e. cycloalkyl and
cycloalkenyl. Unless otherwise specified, these groups contain from 1 to 20 carbon atoms,
with alkenyl groups containing from 2 to 20 carbon atoms. Preferred groups have a total
of up to 10 carbon atoms. Cyclic groups can be monocyclic or polycyclic and preferably *
have from 3 to 10 ring carbon atoms. Exemplary cyclic groups include cyclopropyl, cyclopropylmethyl, cyclopentyl, cyclohexyl and adamantyl.
In addition, the alkyl and alkenyl portions of-X- groups can be unsubstituted or substituted by one or more substituents, which substituents are selected from the groups consisting of alkyl, alkenyl, aryl, heteroaryl, heterocyclyl, arylalkyl, heteroarylalkyl, and heterocyclylalkyl.

The term -haloalkyl- is inclusive of groups that are substituted by one or more halogen atoms, including perfluorinated groups. This is also true of groups that include the prefix -halo--. Examples of suitable haloalkyl groups are chloromethyl, trifluoromethyl, and the like.
The term -aryl- as used herein includes carbocyclic aromatic rings or ring systems. Examples of aryl groups include phenyl, naphthyl, biphenyl, fluorenyl and indenyl. The term -heteroaryl- includes aromatic rings or ring systems that contain at least one ring hetero atom (e.g., 0, S, N). Suitable heteroaryl groups include furyl, thienyl, pyridyl, quinolinyl, isoquinolinyl, indolyl, isoindolyl, triazolyl, pyrrolyl, tetrazolyl, imidazolyl, pyrazolyl, oxazolyl, thiazolyl, benzofuranyl, benzothiophenyl, carbazolyl, benzoxazolyl, pyrimidinyl, quinoxalinyl, benzoimidazolyl, benzothiazolyl, naphthyridinyl, isoxazolyl, isothiazolyl, quinazolinyl, purinyl, and so on.
-Heterocyclyl- includes non-aromatic rings or ring systems that contain at least one ring hetero atom (e,g., 0, S, N) and includes all of the fully saturated and partially unsaturated derivatives of the above mentioned heteroaryl groups. Exemplary heterocyclic groups include pyrrolidinyl, tetrahydrofuranyl, morpholinyl, thiomorpholinyl piperidinyl, piperazinyl, thiazolidinyl, imidazolidinyl, and the like.
The aryl, heteroaryl, and heterocyclyl groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alky l, alkoxy, alkylthio, haloalkyl, haloalkoxy, haloalkylthio, halogen, nitro, hydroxy, mercapto, cyano, carboxy, formyl, aryl, aryloxy, arylthio, arylalkoxy, arylalkylthio, heteroaryl, heteroaryloxy, heteroarylthio, heteroarylalkoxy, heteroarylalkylthio, amino, alkylamino, dialkylamino, heterocyclyl, heterocycloalkyl, alkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, haloalkylcarbonyl, haloalkoxycarbonyl, alkylthiocarbonyl, arylcarbonyl, heteroarylcarbonyl, aryloxycarbonyl, heteroaryloxycarbonyl, arylthiocarbonyl, heteroarylthiocarbonyl, alkanoyloxy, alkanoylthio, arylcarbonyloxy, arylcarbonylthio, arylcarbonylamino, alkylaminosulfonyl, alkylsulfonyl, arylsulfonyl, heteroarylsulfonyl, aryldiazinyl, alkylsulfonylamino, arylsulfonylamino, arylalkylsulfonylamino, alkylcarbonylamino, alkenylcarbonylamino, arylcarbonylamino, arylalkylcarbonylamino. heteroaiylcarbonylamino,heteroarylalkycarbonylamino, alkylsulfonylamino, alkenylsulfonylamino, arylsulfonylamino, arylalkylsulfonylamino, heteroarylsulfonylamino, heteroarylalkylsulfonylamino, alkylaminocarbonylamino,

alkenylaminocarbonylamino, arylaminocarbonylamino, arylalkylaminocarbonylamino, heteroarylaminocarbonylamino, heteroarylalkylcarbonylamino and, in the case of heterocyclyl, oxc. If any other groups are identified as being -substituted- or -optionally substituted-, then those groups can also be substituted by one or more of the above enumerated substituents.
Certain substituents are generally preferred. For example, preferred R1 groups include —R4-~NR3'-S02—Ra—alkyl, —R4—NR3—SO2—R6-aryl, and —R4—NR3—SO2—R^s—heteroaryl wherein the alkyl, aryl and heteroaryl groups can be unsubstituted or substituted and R4 is preferably ethylene or n-butylene, Thiophene and quinoline are preferred heteroaryl groups Preferably no R substituents are present (i.e., n is 0). Preferred R2 groups include hydrogen, alkyl groups having 1 to 4 carbon atoms (i.e., methyl, ethyl, propyl, isopropyl, n-butyl, sec-butyl, isobutyl, and cyclopropylmethyl), methoxyethyl, and ethoxymethyl. For substituted groups such as substituted alkyl or substituted aryl groups, preferred substituents include halogen, nitrile, methoxy, trifluoromethyl, and trifluoromethoxy. One or more of these preferred substituents, if present, can be present in the compounds of the invention in any combination.
The invention is inclusive of the compounds described herein in any of their pharmaceutically acceptable forms, including isomers (e.g., diastereomers and enantiomers), salts, solvates, polymorphs, and the like. In particular, if a compound is optically active, the invention specifically includes each of the compound's enantiomers as well as racemic mixtures of the enantiomers.
Pharmaceutical Compositions and Biological Activity
Pharmaceutical compositions of the invention contain a therapeutically effective amount of a compound of the invention as described above in combination with a pharmaceutically acceptable carrier.
The term -a therapeutically effective amount- means an amount of the compound sufficient to induce a therapeutic effect, such as cytokine induction, antitumor activity, and/or antiviral activity. Although the exact amount of active compound used in a pharmaceutical composition of the invention will vary according to factors known to those of skill in the art, such as the physical and chemical nature of the compound, the nature of





multiple sclerosis; Ommen's syndrome; discoid lupus; Bowen's disease; Bowenoid papulosis; alopecia areata; the inhibition of keloid formation after surgery and other types of post-surgical scars. In addition, these compounds could enhance or stimulate the healing of wounds, including chronic wounds. The compounds may be useful for treating the opportunistic infections and tumors that occur after suppression of cell mediated immunity in, for example, transplant patients, cancer patients and HIV patients.
An amount of a compound effective to induce cytokine biosynthesis is an amount sufficient to cause one or more cell types, such as monocytes, macrophages, dendritic cells and B-cells to produce an amount of one or more cytokines such as, for example, IFN-a, TNF-a, IL-1, IL-6, IL-10 and IL-12 that is increased over the background level of such cytokines. The precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 µg/kg to about 5 mg/kg. The invention also provides a method of treating a viral infection in an animal and a method of treating a neoplastic disease in an animal comprising administering an effective amount of a compound or composition of the invention to the animal. An amount effective to treat or inhibit a viral infection is an amount that will cause a reduction in one or more of the manifestations of viral infection, such as viral lesions, viral load, rate of virus production, and mortality as compared to untreated control animals. The precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 µg/kg to about 5 mg/kg. An amount of a compound effective to treat a neoplastic condition is an amount that will cause a reduction in tumor size or in the number of tumor foci. Again, the precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10µg/kg to about 5 mg/kg.
The invention is further described by the following examples, which are provided for illustration only and are not intended to be limiting in any way.
In the examples below some of the compounds were purified using semi-preparative HPLC, A Waters Fraction Lynx automated purification system was used. The semi-prep HPLC fractions were analyzed using a Micromass LC-TOFMS and the appropriate fractions were combined and centrifuge evaporated to provide the trifluoroacetate salt of the desired compound.

Column: Phenomenex Luna CI8(2), 10 x 50 mm, 5 micron particle size, lOOA pore; flow rate: 25 mL/min.; gradient elution from 5-65% B in 4 min., then 65 to 95 % B in 0.1 min, then hold at 95% B for 0,4 min., where A=0.05 % trifluoroacetic acid/watc-r and B=0.05% trifluoroacetic acid/acetonitrile; fraction collection by mass-selective triggering.

Part A
A solution of 2-(2-aminoethoxy)ethanol (29.0 g, 0.276 mol) in 180 mL of
tetrahydrofuran (THF), under N2, was cooled to O°C and treated with 140 mL of 2N NaOH solution. A solution of di-tert-butyl dicarbonate (60.2 g, 0.276 mol) in 180 mL of THF was then added dropwise over 1 h to the rapidly stirred solution. The reaction mixture was then allowed to warm to room temperature and was stirred an additional 18 h. The THF was then removed under reduced pressure and the remaining aqueous slurry was brought to pH 3 by addition of 150 mL of IM H2SO4 solution. This was then extracted with ethyl acetate (300 mL, 100 mL) and the combined organic layers were washed with H2O (2X) and brine. The organic portion was dried over Na2S04 and concentrated to give tert-butyl 2-(2-hydroxyethoxy)ethylcarbamate as a colorless oil (47.1 g). Part B
A rapidly stirred solution of tert-butyl 2-(2-hydroxyethoxy)ethylcarbamate (47.1 g, 0.230 mol) in 1 L of anhydrous CH2CI2 was cooled to 0°C under N2 and treated with triethylamine (48.0 mL, 0.345 mol). Methanesulfonyl chloride (19.6 mL, 0.253 mol) was

then added dropwise over 30 min. The reaction mixture was then allowed to warm to room temperature and was stirred an additional 22 h. The reaction was quenched by addition of 500 mL saturated NaHC03 solution and the organic layer was separated. The organic phase was then washed with H2O (3 X 500 mL) and brine. The organic portion was dried over Na2S04 and concentrated to give 2*{2-[(tert-butoxycarbonyl)amino]ethoxy}ethyl methanesulfonate as a brown oil (63.5 g). Part C
A stirred solution of 2-{2-[(tert-'butoxycarbonyl)amino]ethoxy}ethyl methanesulfonate (63.5 g, 0.224 mol) in 400 mL of N,N-dimethylformamide (DMF) was treated with NaN} (16.1 g, 0.247 mol) and the reaction mixture was heated to 90°C under N2. After 5 h, the solution was cooled to room temperature and treated with 500 mL of cold H2O. The reaction mixture was then extracted with Et2O (3 X 300 mL). The combined organic extracts were washed with H2O (4 X 100 mL) and brine (2 X 100 mL). The organic portion was dried over MgS04 and concentrated to give 52.0 g of/er/-butyl 2-(2-azidoethoxy)ethylcarbamate as a light brown oil. Part D
A solution of rerr-butyl 2-(2-azidoethoxy)ethylcarbamate (47,0 g, 0.204 mol) in MeOH was treated with 4 g of 10% Pd on carbon and shaken under H2 (3 Kg/cm2 ) for 24 h. The solution was then filtered through a Celite pad and concentrated to give 35.3 g of crude tert-bnty] 2-(2-aminoethoxy)ethylcarbamate as a colorless liquid that was used without further purification. Part E
A stirred solution of 4-chloro-3-nitroquinoline (31.4 g, 0.151 mol) in 500 mL of anhydrous CH2CI2, under N2, was treated with triethylamine (43 mL, 0.308 mol) and tert-butyl 2-(2-aminoethoxy)ethylcarbamate (0.151 mol). After stirring overnight, the reaction mixture was washed with H2O (2 X 300 mL) and brine (300 mL), The organic portion was dried over Na2S04 and concentrated to give a bright yellow solid. Recrystallization from ethyl acetate/hexanes gave 43.6 g of tert-butyl 2-{2-[(3-nitroquinolin-4-yl)amino]ethoxy}ethy]carbamate as bright yellow crystals. Part F
A solution of tert-butyl 2-{2-[(3-nitroquinolin-4-yl)amino]ethoxy}ethylcarbamate (7.52 g, 20,0 mmol) in toluene was treated with 1.5 g of 5% Pt on carbon and shaken

under H2 (3 Kg/cm ) for 24 h. The solution was then filtered through a Celite pad and concentrated to give 6.92 g of crude tert-butyl 2-{2-[(3-aminoquinoIin-4-yl)amino]ethoxy}ethylcarbamate as a yellow syrup. Part G
A solution of tert-butyl 2-{2-[(3-aminoquinolin-4-yl)amino]ethoxy}ethylcarbamate (10.2 g, 29.5 mmol) in 250 mL of anhydrous CH2CI2 was cooled to O°C and treated with triethylamine (4.18 mL, 30.0 mmol). Methoxypropionyl chloride (3,30 mL, 30.3 mmol) was then added dropwise over 5 min. The reaction was then warmed to room temperature and stirring was continued for 1 h. The reaction mixture was then concentrated under reduced pressure to give an orange solid. This was dissolved in 250 mL of EtOH and 12.5 mL of triethylamine was added. The mixture was heated to reflux and stirred under N2 overnight. The reaction was then concentrated to dryness under reduced pressure and treated with 300 mL of Et2O. The mixture was then filtered and the filtrate was concentrated under reduced pressure to give a brown solid. The solid was dissolved in 200 mL of hot MeOH and treated with activated charcoal. The hot solution was filtered and concentrated to give 11.1 g of tert-butyl 2-{2-[2-(2-methoxyethyl)-lH-imida2o[4,5-c]quinolin-l-yl]ethoxy}ethylcarbamate as a yellow syrup. Part H
A solution of tert-butyl 2-{2-[2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethylcarbamate (10.22 g, 24.7 mmol) in 250 mL of CHCI3 was treated with 3-chloroperoxybenzoic acid (MCPBA, 77%, 9.12 g, 40.8 mmol). After stirring 30 min, the reaction mixture was washed with 1% Na2C03 solution (2 X 75 mL) and brine. The organic layer was then dried over Na2S04 and concentrated to give 10.6 g of tert-butyl 2-{2*[2-(2-methoxyethyl)-5-oxido-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethylcarbamate . as an orange foam that was used without further purification. Parti
A solution oftert-butyl 2-{2-[2-(2-methoxyethyl)-5-oxido-lH-imidazo[4,5' c]quinolin-l-yl]ethoxy}ethylcarbamate (10.6 g, 24.6 mmol) in 100 mL of 1,2-dichloroethane was heated to 60°Cand treated with 10 mL of concentrated NH4OH solution. To the rapidly stirred solution was added solid p-toluenesulfonyl chloride (7,05 g, 37,0 mmol) over a 10 min period. The reaction mixture was treated with an additional 1

mL concentrated NH4OH solution and then sealed in a pressure vessel and heating was continued for 2 h. The reaction mixture was then cooled and treated with 100 mL of CHCI3, The reaction mixture was then washed with H2O, 1% Na2C03 solution (2X) and brine. The organic portion was dried over Na2S04 and concentrated to give 10.6 g of tert-butyl 2-{2-[4-amino-2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethylcarbamate as a brown foam. Part J
Tert-butyl 2-{2-[4-amino-2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethylcarbamate (10.6 g, 24.6 mmol) was treated with 75 mL of 2M HCl in EtOH and the mixture was heated to reflux with stirring. After L5 h, the reaction mixture was cooled and filtered to give a gummy solid. The solid was washed EtOH and Et20 and dried under vacuum to give the hydrochloride salt as a light brown solid. The free base was made by dissolving the hydrochloride salt in 50 mL of H2O and treating with 10% NaOH solution. The aqueous suspension was then concentrated to dryness and the residue was treated with CHCI3. The resulting salts were removed by filtration and the filtrate was concentrated to give 3.82 g of l-[2-(2-aminoethoxy)ethyl]-2-(2-methoxyethyl)-lH-imida2o[4,5'C]quinoIin-4-amine as a tan powder. MS330(MH-H)+;
1HNMR(300MH2,DMSO-d6)δ 8.10(d,J=8.1 Hz, 1 H); 7.66 (d,J = 8.2 Hz, 1 H); 7.40 (m, 1 H); 7,25 (m, 1 H); 6.88 (br s, 2 H); 4.78 (t, J = 5.4 Hz. 2 H); 3.89 (t, J = 4.8 Hz, 2 H); 3.84 (t, J = 6.9 Hz, 2 H); 3.54 (t,7 = 5.4 Hz, 2 H); 3.31 (s, 3 H); 3.23 (t, J = 6.6 Hz, 2 H); 2.88 (t, J =5.3 Hz, 2 H). Part K
l-[2-(2-Aminoethoxy)ethyI]-2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-4-amine (750 mg, 2.28 mmol) was dissolved in 30 mL of anhydrous CH2CI2 and cooled to 0°C under N2. To the stirred solution were added Et3N (0,64 mL, 4.56 mmol) and methanesulfonyl chloride (176 µL, 2.28 mmol) and the reaction was allowed to warm to room temperature over 2 h. The reaction mixture was then quenched by addition of saturated NaHC03 solution (30 mL). The organic layer was separated and washed with H2O (3 X 25 mL) and brine, dried over Na2S04 and concentrated under reduced pressure to give a tan foam. The foam was dissolved in a minimum amount of MeOH and Et20 was added and a solid percipitated from the solution. The off-white solid was isolated bv

filtration and dried to yield 385 mg of N-(2-{2-[4-amino-2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethyl)methanesulfonamide. m.p. 114,0-117.0 °C ; MS 408 (M + H)+;
1H NMR (300 MHz, DMSO-d6) δ 8.05 (d, J = 7.6 Hz, 1 H); 7.61 (d, J = 8.5 Hz, 1 H);
7.42 (t, J = 8.5 Hz, 1 H); 7.24 (t, J = 7.0 Hz, 1 H); 6.99 (t, J - 4.4 Hz, 1 H); 6.51 (s, 2
H);4.76 (t, J = 5.0 Hz, 2 H); 3.88-3.81 (m, 4 H); 3.41 (t, J = 5.4 Hz, 2 H); 3.31 (s, 3 H);
3.23 (t, J = 6.9 Hz, 2 H); 3.04-2.99 (m, 2 H); 2,81 (s, 3 H);
13C (75 MHz, DMSO-d6) 151.9, 145.0, 132.7, 126.7, 126.6, 121.5, 120.5, 115.1, 70.5,
70.2,69.3,58.5,45.4,42.4,27.6.
Anal. Calcd for C18H25N5O4S.O.23 H2O: %C, 52.52; %H, 6.23; %N, 17.01. Found: %C,
52.55; %H, 6.17; %N, 16.66

Part A
l-[2-(2-Aminoethoxy)ethyl]-2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-4-amine (10.0 g, 27.3 mmol) was dissolved in 50 mL of trifluoroacetic acid and treated with Pt02 (1.0 g). The reaction mixture was shaken under H2 (3 Kg/cm2). After 4 d, an additional 0.5 g of Pt02 was added and hydrogenation was continued for an additional 3 d. The reaction was then filtered through Celite and concentrated under reduced pressure to give a brown oil. The oil was dissolved in 200 mL of H2O then made basic (pH-l 1) by addition of 10% NaOH solution. This was then extracted with CHCI3 (5 X 75 mL) and the combined organic layers were dried over Na2S04 and concentrated to give 5.17 g of l-[2-

(2-aminoethoxy)ethyl]-2-(2-methoxyethyl)-6,7,8,9-telTahydro-lH-imidazo[4,5-c]quinolin-
4-amine as a tan solid.
MS 334 (M + H)+
'H NMR (300 MHz, CDCI3) 5 5.19 (s, 2 H); 4.49 (t, J = 5.4 Hz, 2 H); 3.84 (t, J = 6.6 Hz,
2 H); 3.71 (t, J = 5.4 Hz, 2 H), 3.36 (t, J = 5.2 Hz, 2 H); 3.28 (s, 3 H); 3.15 (t, J = 6.6 11.^,
2 H); 2.95 (m, 2 H); 2.82 (m, 2 H); 2.76 (t, J = 5.1 Hz, 2 H); 1.84 (m, 4 H), 1.47 (br s, 2
H).
Part B
l-[2-{2-Aminoethoxy)ethyl]-2-(2-methoxyethyl)-6,7,8,9-tetrahydro-lH-imidazo[4,5-c]quinolin-4-amine (1.00 g, 3.00 mmol) was dissolved in 30 mL of anhydrous CH2CI2 and cooled to 0°C under N2. To the stirred solution were added Et3N (0.84 mL, 6.00 mmol) and methanesulfonyl chloride (232 \xL, 3.00 mmol) and the reaction was allowed to warm to room temperature overnight. The reaction mixture was then quenched by addition of saturated NaHCO3 solution (30 mL). The organic layer was separated and washed with H2O and brine, dried over Na2S04 and concentrated under reduced pressure to give a yellow solid. The solid was triturated with Et20 and a few drops of MeOH. The resulting white powder was isolated by filtration and further purified by column chromatography (SiOi, 3% MeOH/CHCl3 saturated with aqueous NH4OH) to give 389 mg of N-(2-{2-[4-amino-2-(2-methoxyethyl)-6,7,8,9-tetrahydro-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethyl)methanesulfonamide as a white powder, m.p. 151.0-153.0 °C; MS 412(M + H)+6;
'H NMR (300 MHz, DMSO-d6) δ 7.00 (t, J = 5.4 Hz, 1 H); 5.68 (s, 2 H); 4.44 (t, 7=5.1 Hz, 2 H); 3.77 (t, J = 6.8 Hz, 2 H); 3.68 (t, J = 5.0 Hz, 2 H); 3.39 (t, J = 5.8 Hz, 2 H); 3.28 (s, 3 H); 3.11-2.99 (m, 4 H); 2.92 (m, 2 H), 2.82 (s, 3 H); 2.65 (m, 2 H); 1.75 (m, 4
H);
13C (75 MHz, DMSO-d6) 151.3, 149.3, 146.3, 138.4, 124.9, 105.6, 70.6, 70.5, 70.1, 44.5,
42.4,32.7,27.6,23.8,23.1,23.0.
Anal. Calcd for C18H29N5O4S: %C, 52.54; %H, 7.10; %N, 17.02. Found: %C, 52.47; %H,
7.22; %N, 16.83.


Part A
Sodium hydride (60% oil dispersion, 9.1 g, 228 mmol) was placed in a round bottom flask and washed with hexanes (3X) under N2. The dried sodium hydride was treated with 800 mL of anhydrous THF. A solution of tert-butyl 2-(2-azidoethoxy)ethylcarbamate (41.9 g, 182 mmol) in 200 mL of THF was then added to the stirred sodium hydride solution over 40 min. After addition was complete, the reaction was stirred an additional 20 min followed by addition of methyl iodide (13.6 mL, 218 mmol). After stirring overnight, the reaction was quenched with 300 mL of saturated NaHC03 solution. The reaction mixture was then treated with 200 mL of H2O and 1 L of Et20. The organic phase was separated and washed with H2O and brine. The organic portion was then dried over MgS04 and concentrated under reduced pressure to give 41.9 g of tert-butyl 2-(2-azidoethoxy)ethyl(methyl)carbamate as a yellow liquid. Part B
A solution of tert-butyl 2-(2-azidoethoxy)ethyl(methyI)carbamate (41.9 g, 170 mmol) in 600 mL of MeOH was treated with 2.5 g of 10% Pd on carbon and shaken under H2 (3 Kg/cm^) for 24 h. The solution was then filtered through a Celite pad and concentrated to give 37.2 g of crude tert-butyl 2-(2-aminoethoxy)ethyl(methyl)carbamate as a light yellow liquid. Part C
A stirred solution of 4-chloro-3-nitroquinoline (32.3 g, 155 mmol) in 400 mL of anhydrous CH2CI2, under N2, was treated with triethylamine (43.1 mL, 310 mmol) and

tert-butyl 2-(2-aminoethoxy)ethyl(nethyl(carbamete(37.2 g, mmol). After sturring overnight, the reaction mixture was washed with H2O (2 X 300 mL) and brine (300 mL). The organic portion was dried over Na2S04 and concentrated to giv 2 a brown oil. Column chromatography (Si02, 33% ethyl acetate/hexanes-67% ethyl acetate/hexanes) gave 46.7 g of tert-butyl methyl(2-{2'[(3-nitroquinolin-4-yI)amino]ethoxy}ethyl)carbamate as a yellow solid. Part D
A solution of tert-butyl methyl(2-{2-[(3'nitroquinoIin-4-yl)amino]ethoxy}ethyl)carbamate (6.56 g, 16.8 mmol) in 75 mL of toluene was treated with 0.5 g of 5% Pt on carbon and shaken under H2 (3 Kg/cm ) for 24 h. The solution was then filtered through a Celite pad and concentrated to give 6.8 g of crude tert-butyl 2-{2-[(3-aminoquinolin-4-yl)amino]ethoxy}ethyl(methyl)carbamate as an orange syrup which was carried on without further purification. PartE
A solution of tert-butyl 2-{2-[(3-aminoquinolin-4-yl)amino]ethoxy}ethyl(methyl)carbamate (6.05 g, 16.8 mmol) in 200 mL of anhydrous CH2CI2 was cooled to 0°C and treated with triethylamine (2,40 mL, 17.2 mmol). Methoxypropionyl chloride (1.72 mL, 17.2 mmol) was then added dropwise over 5 min. The reaction was then warmed to room temperature and stirring was continued for 3 h. The reaction mixture was then concentrated under reduced pressure to give an orange solid. This was dissolved in 200 mL of EtOH and 7.2 mL of triethylamine was added. The mixture was heated to reflux and stirred under N2 overnight. The reaction was then concentrated to dryness under reduced pressure and treated with 300 mL of Et2O. The mixture was then filtered and the filtrate was concentrated under reduced pressure to give a brown solid. This was dissolved in 300 mL of CH2CI2 and washed with H2O and brine. The organic portion was dried over Na2S04 and concentrated under reduced pressure to give a brown oil. The oil was dissolved in 100 mL of hot MeOH and treated with activated charcoal. The hot solution was filtered and concentrated to give 7,20 g oftert-butyl 2' {2-[2-(2-methoxyethyl)-1 H-imidazo[4,5-c]quinolin-1-yl]ethoxy}ethyl(methyl)carbamate as a yellow syrup. Part F

A solution of/er/-butyl 2-{2-[2-(2-methoxyethyl)-lH-iniidazo[4,5-c]quinolin-l-ylJethoxy}ethyl(methyl)carbamate (7.20 g, 16.8 mmol) in 200 mL of CH2CI2 was treated with MCPBA (77%, 4,32 g, 19.3 mmol). After stirring 6 h, the reaction mixture was treated with saturated NaHCO3 solution and the layers were separated. The organic portion was washed with H2O and brine then dried over Na2S04 and concentrated to give 7.05 g of tert-butyl 2-{2-[2-(2-methoxyethyl)-5-oxido-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethyl(methyl)carbamate as a light brown solid. Part G
A solution of tert-butyl 2-{2-[2-(2-methoxyethyl)-5-oxido-lH'-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethyl(methyl)carbamate (7.05 g, 15.9 mmol) in 100 mL of 1,2-dichloroethane was heated to 80°C and treated with 5 mL of concentrated NH4OH solution. To the rapidly stirred solution was added solid p-toluenesulfonyl chloride (3.33 g, 17,5 mmol) over a 10 min period. The reaction mixture was treated with an additional 5 mL concentrated NH4OH solution and then sealed in a pressure vessel and heating was continued for 4 h. The reaction mixture was then cooled and treated with 100 mL of CH2CI2. The reaction mixture was then washed with H2O, 1% Na2C03 solution (3X) and brine. The organic portion was dried over Na2S04 and concentrated to give 6,50 g of tert-butyl 2-{2-[4-amino-2-(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}cthyl(methyl)carbamate as a brown oil Part H
Ter/'buty!2-{2-[4-amino-2-(2-methoxyethyl)'lH-imidazo[4,5-c]quinolin-l-yl]ethoxy}ethyl(methyl)carbamate (6.50 g, 14.7 mmol) was dissolved in 100 mL of EtOH and treated with 20 mL of 2M HCl in EtOH and the mixture was heated to reflux with stirring. After 6 h, the reaction mixture was cooled and filtered to give a gummy solid. The solid was washed with EtOH and Et20 and dried under vacuum to give the hydrochloride salt as a light brown powder. The free base was made by dissolving the hydrochloride salt in 50 mL of H2O and treating with 5 mL of concentrated NH4OH. The aqueous suspension was extracted with CHCI3 (5 X 50 mL). The combined organic layers were dried over Na2S04 and concentrated to give 3.93 g of 2-(2-methoxyethyl)-l-{2-[2-(methylamino)ethoxy]ethyl}-lH-imidazo[4,5-c]quinolin-4-amine as a tan powder. MS 344 (M + H)+;

'H NMR (300 MHz, DMSO-d6) δ 8.07 (d, J = 7.7 Hz, 1 H); 7.62 (dd, 7 = 1.0, 8.3 Hz, 1 H); 7.42 (ddd, J = 1.0, 7.1, 8.2 Hz, 1 H); 7.22 (ddd, 7 = 1.1, 7.1, 8.2 Hz, 1 H); 6.49 (s, 2 H); 4.75 (t, y = 5,1 Hz, 2 H); 3.83 (t, J = 6.8 Hz. 4 H); 3.35 (t, J = 5.6 Hz, 2 H); 3.30 (s, 3 H); 3.21 (t,y = 6.9 Hz, 2 H); 2.45 (t,J = 5.6 Hz, 2 H); 2.12 (s, 3 H). Part I
2-(2-Methoxyethyl)-l-{2-[2-(methylamino)ethoxy]ethyl}-lH-imidazo[4,5-c]quinolin-4-amine (1.00 g, 2.92 mmol) was dissolved in 30 mL of anhydrous CH2CI2 and cooled to 0°C under N2. To the stirred solution were added Et3N (0.81 mL, 5.81 mmol) and methanesulfonyl chloride (226µL, 2.92 mmol) and the reaction was allowed to warm to room temperature overnight. The reaction mixture was then quenched by addition of saturated NaHCOa solution (30 mL) and CH2CI2 (30 mL). The organic layer was separated and washed with H2O and brine, dried over Na2S04 and concentrated under reduced pressure. Crystallization of the residue from EtOAc and CH2CI2 gave 756 mg of N-(2- {2-[4-amino-2-(2-methoxyethyl)- lH-imidazo[4,5-c]quinolin-1 -yl]ethoxy} ethyl)-N-
methylmethanesulfonamide as tan crystals, m.p. 145.0-146.5 °C;
MS 422 (M + H)+
'H NMR (300 MHz, DMSO-d6) δ 8.06 (d, J = 7.8 Hz, 1 H); 7.61 (dd, J = 0.9, 8.3 Hz, 1
H); 7.42 (t, J = 7.2 Hz, 1 H); 7.23 (ddd, J = 1.0, 7.0, 8.0 Hz, 1 H); 6.50 (s, 2 H); 4.77 (t, J
- 5.0 Hz, 2 H); 3.87 (t, / = 5.0 Hz, 2 H), 3.83 (t, J = 6.8 Hz, 2 H); 3.48 (t, J = 5.5 Hz, 2
H); 3.30 (s, 3 H); 3.22 (t, J = 6.8 Hz, 2 H); 3.13 (t, 7 = 5.5 Hz, 2 H); 2.77 (s, 3 H); 2.63 (s,
3H);
13C NMR (75 MHz,DMSO-d6)δ 153.9, 153.8, 147.0, 134.6, 128.6, 128.5, 123.4, 122.5,
117.0, 72.4, 71.2, 60.4, 51.1, 47.3, 37.3, 37.2, 29.6.
Anal. Calcd for C19H27N5O4S: %C, 54.14; %H, 6.46; %N, 16.61. Found: %C, 53.92; %H,
6.32; %N, 16.47.


Part A
2-(2-Methoxyethyl)-l-{2-[2-(methylamino)ethoxy]ethyl}-lH-imidazo[4,5-
c]quinolin-4-amine (4.22 g, 12.3 mmol) was dissolved in 25 mL of trifluoroacetic acid and
treated with PtOa (0.5 g). The reaction mixture was shaken under H2 (3 Kg/cm ). Alter 4
d, an additional 0,5 g of PtOz was added and hydrogenation was continued for an
additional 3 d. The reaction mixture was then filtered through Celite and concentrated
under reduced pressure to give a yellow oil. The yellow oil was dissolved in 50 mL of
H2O and extracted with 50 mL of CHCI3. The organic portion was removed and
discarded. The aqueous portion was then made basic (pH~12) by addition of 10% NaOH
solution. This was then extracted with CHCI3 (6 X 50 mL) and the combined organic
layers were dried over Na2S04 and concentrated to a brown oil. The brown oil was
dissolved in 100 mL of hot MeOH and treated with 1 g of activated charcoal. The hot
solution was filtered through Celite and concentrated to dryness. The resulting gummy
solid was concentrated several times with EtaO to give 3,19 g of 2-(2-methoxyethyl)-1 - {2
[2-(methylamino)ethoxy]ethyl}-6,7,8,9-tetrahydro-lH-imidazo[4,5-c]quinolin-4-aminea,
an off-white powder.
MS 348 (M + H)+
'H NMR (300 MHz, CDCI3) 5 4,84 (s, 2 H); 4.48 (t, J = 5.7 Hz, 2 H); 3.84 (t, y = 6.7 .
2 H); 3.70 (t,J = 5.7 Hz, 2 H); 3.46 (t, J= 5.1 Hz, 2 H); 3.36 (s, 3 H); 3,14 (t, J - 6.7 HZ.
2 H); 2,96 (m, 2 H); 2.83 (m, 2 H); 2.65 (t,J= 5,1 Hz, 2 H); 2,36 (s, 3 H); 1.85 (m, 4 11)

PartB
(2-(2-Methoxyethyl)-l-{2-[2-(methylammo)ethoxy]ethyl}-6,7,8,9-tetrahydro-lH. imidazo[4,5-c]quinolin-4-amine (750 mg, 2.16 mmol) was dissolved in 30 mL of anhydrous CH2CI2 and cooled to O° under N2. To the stirred solution were added Et^N (0.60 mL, 4.32 mmol) and methanesulfonyl chloride (167 µL, 2.16 mmol) and the reaction was allowed to warm to room temperature over 3 h. The reaction mixture was then quenched by addition of saturated NaHCO3 solution (30 mL) and CH2CI2 (30 mL). The organic layer was separated and washed with H2O and brine, dried over Na2S04and concentrated under reduced pressure. Purification by column chromatography (SiOz, 3-5% MeOH/CHCl3 saturated with aqueous NH4OH) gave the product as a colorless glass. The material was then concentrated with 150-propyl alcohol to give a syrup which solidified upon standing in the freezer. The solid was dried under vacuum to give 437 mg of N-(2-{2-[4-amino-2-(2-methoxyethyl)-6,7,8,9-tetrahydro-lH-imidazo[4,5'c]quinolin-l-yl]ethoxy}ethyl)-N-methylmethanesulfonamide as off-white crystals, m.p, 115.3-117.8 °C; MS 426 (M + H)+;
1H NMR (300 MHz, DMSO-d3) δ 5.65 (s. 2 H); 4.44 (t, J = 5.2 Hz, 2 H); 3.76 (t, J - 6.9 Hz, 2 H), 3.70 (t, J = 5.3 Hz, 2 H); 3.47 (t, J = 5.5 Hz, 2 H); 3.27 (s, 3 H); 3.15 (t, / = 5.5 Hz, 2 H); 3.08 (t, / - 6.9 Hz, 2 H); 2.93 (m, 2 H); 2.78 (s, 3 H); 2.65 (s, 3 H); 2.64 (m, 2 H);1.74(m,4H);
13 NMR (75 MHz, DMSO-d6) δ 15L2, 149.3, 146.3, 138.5, 124.9, 105,6, 70,6, 70.5, 69.2, 58.4,49.2,44.5, 35.4, 35.2, 32.7,27.6,23.8,23,1,23.0.
Anal. Calcd for Ci9H27N5O4S-0.40 C3H8O: %C. 53.97; %H, 7.67; %N, 15.58. Found: %C, 53.71; %H, 7,48; %N, 15.77.


Under a nitrogen atmosphere, chloropropylsulfonyl chloride (0.05 ml, 0.46 mmol) was added dropwise to a solution of l-[2-(2-aminocthoxy)ethyl]-2-butyl-lH-imidazo[4,5-' c]quinolin-4-amine (0.12 g, 0.37 mmol) and triethylamine (0.065 ml, 0.46 mmol) in dichloromethane (5 ml). The reaction was stirred for 20 hours followed by removal of the solvent in vacuo. The resulting off-white solid was dissolved in N,N-dimethylformamide (5mL) and l,8-diazabicyclo[5.4.0]undec-7-ene (0.087ml, 0.58 mmol) was added. The reaction was stined for 18 hours under an atmosphere of nitrogen and then quenched with water and extracted with dichloromethane (2X). The organic fractions were combined, washed with water followed by brine, dried (Na2S04), filtered, and concentrated in vacuo to provide an off-white solid. Recrystallization from ethyl acetate yielded 0,068 g of 2-butyl-l-{2-[2-(l,l-dioxidoisothiazolidin-2-yl)ethoxy]ethyl}-lH-imidazo[4,5-C]quinolin-4-amine as off-white crystals, m.p. 152-154°C.
'H-NMR (300MHz, DMSO-d6): δ 8.06 (d, J = 8.1Hz, 1H), 7.62 (d, J = 7.9Hz, 1H), 7.42 (t, J = 7.6Hz, 1H), 7.23 (t, J = 7.5Hz, 1H), 6,52 (s, 2H), 4.73 (t, J = 4,99Hz, 2H), 3.86 (t, J = 5.0Hz, 2H), 3.46 (t, J - 5.3Hz, 2H), 3.07 (t, J = 7.66Hz, 2H), 2.97-2.87 (m, 6H), 2.04 (quintet, J = 6.8Hz,2H), LSI (quintet, J = 7.6H2, 2H), 1.46 (sextet, J = 7,4Hz, 2H), 0.96 (t, J = 7.3H2, 3H);
13C-NMR(75MHz,DMSO-d6): 5 154.6, 152.9, 145.1, 133.0, 126.8, 126.6, 121.6, 120.8, 115.3, 69.2, 69.1, 47.0, 45.5, 45.0, 43.7, 29.3, 26.2, 21.9, 18.1, 13.7;

Anal calcd for C2iH29N5O3S*0.25H2O: %C, 57.84; %H, 6.82; %N, 16,06; %S, 7.35. Found: %C, 57.90; %H, 6,79; %N, 15,92; %S, 7.55.
Examples 6 - 26
Part A
A solution of tert-butyl 2-{2-[(3-aminoquinolm-4-yl)amino]ethoxy}ethylcarbamate (3*46 g, 10.0 mmol) in 50 mL of toluene was treated with triethylorthovalerate (2.5 mL, 14.5 mmol) and the reaction mixture was healed to reflux. A 25 mg portion of pyridinium hydrochloride was then added and refluxing was continued for 4 h. The reaction was then concentrated to dryness under reduced pressure. The residue was dissolved in 50 mL of CH2CI2 and washed with saturated NaHCOj, H2O and brine. The organic portion was dried over Na2S04 and concetrated to give a green oil. The green oil was dissolved in 50 mL of hot MeOH and treated with activated charcoal. The hot solution was filtered and concentrated to give 4.12 g of tert-butyl 2-[2'(2-butyi-lH-imidazo[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate as a yellow oil. Part B
A solution of tert-butyl 2*[2-(2-butyl-lH-imida2o[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate (4.12 g, 10.0 mmol) in 50 mL of CH2CI2 was treated with 3-chloroperoxybenzoic acid (MCPBA, 77%, 2;5 g, 11.2 mmol). After stirring for 5 h, the reaction mixture was treated with saturated NaHCO3 solution and the layers were separated. The organic portion was washed with H2O and brine then dried over Na2S04 and concentrated to give 3.68 g of tert-butyl 2-[2-(2-butyl-5-Oxido-lH-imidazo[4,5-c]quinolin-'l-yl)ethoxy]ethylcarbamate as a light brown foam. Part C
A solution of tert-butyl 2-[2-(2-butyl-5-oxido-li/-imidazo[4,5-c]quinolin-1-yl)ethoxy]ethylcarbamate (3.68 g, 8.60 mmol) in 100 mL of 1,2-dichloroethane was heated to 80 -C and treated with 10 mL of concentrated NH4OH solution. To the rapidly stirred solution was added solid p-toluenesulfonyl chloride (1.87 g, 9.81 mmol) over a 10 min period. The reaction mixture was then sealed in a pressure vessel and heating was

continued for 2 h. The reaction mixture was then cooled and treated with 100 mL of CH2CI2. The reaction mixture was then washed with H20,1% Na2C03 solution (3X) and brine. The organic portion was dried over Na2S04 and concentrated to give 3.68 g of tert-butyl 2-[2-(4-amino-2-butyl-1H-imidazo[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamatc as a
light brown foam. Part D
Tert-butyl 2-[2-(4-amino-2-butyl-lH-imidazo[4,5"c]quinolin-1' yl)ethoxy]ethylcarbamate (3.68 g, 8.60 mmol) was suspended in 20 mL of 2M HCl in EtOH and the mixture was heated to reflux with stirring. After 3 h, the reaction mixture was concentrated to give a solid. The solid was triturated with hot EtOH (50 mL) and filtered to give 2,90 g of the product as the hydrochloride salt. The free base was made by dissolving the hydrochloride salt in 50 mL of H2O and treating with 5 mL of concentrated NH4OH. The aqueous suspension was extracted with CH2CI2 (3 X 50 mL). The combined organic layers were dried overNa2S04 and concentrated to give l-[2-(2' aminoethoxy)ethyl]-2-butyl-lH-imidazo[4,5-c]quinolin-4-amine as a tan powder. MS 328 (M + H)+
1H NMR (300 MHz, CDCI3) 5 7.95 (d.J - 8.3 Hz, 1 H); 7.83 (d, J = 8.4 Hz, 1 H); 7.50 (m, 1 H); 7.30 (m, 1 H); 5.41 (s, 2 H); 4.69 (t, J = 5.6 Hz, 2 H); 3.93 (t, J = 5.6 Hz, 2 H); 3.39(t,J = 5.1 Hz, 2 H); 2.97 (t, y - 7.9 Hz, 2 H); 2.76 (t, 7=5.1 Hz, 2 H); 1.89(m,2H); 1.52 (m, 2 H); 1.26 (br s, 2 H); 1.01 {t,J=13 Hz, 3 H), Part E
The compounds in the table below were prepared according to the synthetic method of step (7) of Reaction Scheme II above using the following general method.
The sulfonyl chloride or the sulfamoyl chloride (1.1 eq.) was added to a test tube containing a solution of l-[2-(2-aminoethoxy)ethyl]-2-butyl-lH-imidazo[4,5-c]quinolin-4-amine (25 mg) in dichloromethane (5 mL). The test tube was capped and then placed on a shaker at ambient temperature for 18 - 20 hr. The solvent was removed by vacuum centrifugation. The residue was purified by semi-preparative HPLC using the method described above. The products were verified by accurate mass and 1H NMR. The table below shows the structure of the free base and the observed accurate mass (M -f H).
















Examples 27 - 39 Part A
Using the general method of Part A of Examples 6 - 26, 4-piperidineethanol (! 0 L 77.4 mmol) was reacted with di-tert-bntyl dicarbonate (17.7 g, 81.3 mmol) to provide i \ 1 g of tert-butyl 4-(2-hydroxyethyl)piperidine-l-carboxylate as a clear oil. Part B
Iodine (7,97 g) was added in three portions to a solution of imidazole (3.89 g, 57. i mmol) and triphenylphosphine (14.98 g, 57.1 mmol) in dichloromethane (350 mL). After 5 minutes a solution of the material from Part A in dichloromethane (70 mL) was added. The reaction mixture was stirred at ambient temperature overnight. More iodine (7.97 g) was added and the reaction was stirred at ambient temperature for 1 hr. The reaction mixture was washed with saturated sodium thiosulfate (2X) and brine, dried over sodimn sulfate, filtered and then concentrated under reduced pressure to provide an oily residue The residue was purified by column chromatography (silica gel eluting with 20% ethyl acetate in hexanes) to provide 15,52 g of tert-butyl 4-(2-iodoethyl)piperidinc-l-carboxylate as a pale yellow oil. Parte
Under a nitrogen atmosphere, 2-(lH-imidazo[4,5-c]quinolin-l-yl)butan-l -ol (6,5 g 26.9 mmol) was added in three portions to a suspension of sodium hydride (1 4 g of 60°C, 35,0 mmol) in anhydrous N,N-dimethylformamide. The reaction mixture was allowed to stir for 45 minutes by which time gas evolution had ceased, Tert-butyl- 4-(2-iodoethyl)piperidine-l-carboxylate (10.05 g, 29.6 mmol) was added dropwise over a period of 15 minutes. The reaction mixture was allowed to stir at ambient temperature for 2*5 hrs; then it was heated to 100°C and stirred overnight. Analysis by HPLC showed that the reaction was about 35% complete. Saturated ammonium chloride solution was added., the resulting mixture was allowed to stir for 20 minutes and then it was extracted w ith ethyl acetate (2X). The ethyl acetate extracts were washed with water (2X) and then with brine, combined, dried over sodium sulfate, filtered and then concentrated under reduced pressure to provide a brown oil. The oil was purified by column chromatography (silica gel eluting sequentially with 30% ethyl acetate in hexanes, 50% ethyl acetate in hexanes, and ethyl acetate) to provide 2,2 g of tert-butyl 4-{2-[2-(lH-imidazo[4,5-c]quinolin-l -yl)butoxy]ethyl} piperidine-1 -carboxylate.

Part D
Using the general method of Examples 6-26 Part H, the material from Part C was oxidized to provide tert-butyl 4-{2-[2-(5-oxido-lH-imidazo[4,5-c]quinolin-l-yl)butoxy]ethyl}piperidine"l-carboxylate as an oil-Part E
Ammonium hydroxide solution (20 mL) was added to a solution of the material from Part D in dichloromethane (20 mL). A solution of tosyl chloride (0.99 g, 5.2 mmol) in dichloromethane (10 mL) was added over a period of 5 minutes. The resulting biphasic reaction mixture was allowed to stir overnight. The reaction mixture was diluted with chloroform and saturated sodium bicarbonate solution. The layers were separated. The organic layer was dried over sodium sulfate, filtered and then concentrated under reduced pressure to provide a brown glass. This material was purified by column chromatography (silica gel eluting first with 50% ethyl acetate in hexanes and then with ethyl acetate) to provide 1.0 g oftert-hnty\ 4-{2-[2-(4-amino-lH-imidazo[4,5-c]quinolin*l-yl)butoxy]ethyl}piperidine-l-carboxylate as pale yellow glassy foam. Part F
Under a nitrogen atmosphere, tert-hutyl 4- {2-[2-(4-amino-1H-imidazo[4.5-c]quinolin-l-yl)butoxy]ethyl}piperidine-l-carboxylate (LOO g, 2.1 mmol) and 2N ethanolic hydrochloric acid (10 ml, 20 mmol) were combined and the solution was stirred at ambient temperature for 14 hours. The solvent was removed in vacuo and the resulting tan solid was dissolved in water. Saturated aqueous sodium carbonate was added until the pH reached 10, After extraction with dichloromethane (3X), the organic fractions were combined, washed with brine, dried (Na2S04), filtered, and the majority of the solvent was removed in vacuo, Hexane was added to form a precipitate. Vacuum filtration yielded 0.5 g of l-{l-[(2-piperidin-4-ylethoxy)methyl]propyl}-lH-imidazo[4,5-c]quinolin-4-amine as a tan powder.
1H-NMR (300MHz, DMS0-d6): 5 8.34 (bs, 1H), 8.19 (d. J = 8.49, 1H), 7.61 (dd, J = 8.31, L13, 1H), 7.45-7.39 (m. 1H), 7.25-7,19 (m, 1H), 6.55 (s, 2H), 5.25-5.15 (m, 1H), 4.00-3.80 (m, 2H), 3.5-3.3 (m, 2H), 2.8-2.64 (m, 2H), 2.22-2.11 (m. 2H), 2.09-1.99 (m, 2H), 1.8-1.63 (bs, 1H), 1.37-1.0 (m, 5H), 0.95-0.7 (m, 5H);
13C-NMR (75MHz, DMS0-d6): 5 152.8, 145.8, 140.6, 133.0, 127.8, 127.0, 126.9, 121.3, 121.0, 115.5, 7L8, 68.1, 58,4,46.1, 36.3, 33.1, 32.7,24.5, 9.9;

MS (CI) m/e 368.2459 (368.2450 calcd for C2]H3oN50). Part G
The compounds in the table below were prepared according to the synthetic method of step (7) of Reaction Scheme II above using the following general method.
The sulfonyl chloride or the sulfamoyl chloride (1.1 eq.) was added to a test tube containing a solution of l-{l'[(2-piperidin"4-ylethoxy)methyl]propyl}-lH-imidazo[4,5-c]quinolin-4-amine (25 mg) in dichloromethane (5 mL). The test tube was capped and then placed on a shaker at ambient temperature for 20 hr. The solvent was removed by vacuum centrifugation. The residue was purified by semi-preparative HPLC using the method described above. The products were verified by accurate mass and 1H NMR. The table below shows the structure of the free base and the observed accurate mass (M + H).










Examples 40 -49
Part A
A solution of tert-butyl 2-{2-[(3-aminoquinolin-4-
yl)amino]ethoxy}ethylcarbamate (6.92 g, 20.0 mmol) in 100 mL of toluene was treated
with triethylorthoformate (4.65 mL, 28.0 mmol) and the reaction mixture was heated to
reflux. A 100 mg portion of pyridinium hydrochloride was then added and refluxing was
continued for 2 h. The reaction was then concentrated to dryness under reduced pressure.
The residue was dissolved in 200 mL of CH2CI2 and washed with saturated NaHCOa, H2O
and brine. The organic portion was dried over Na2S04 and concentrated to give a green
oil. The green oil was dissolved in 200 mL of hot MeOH and treated with 10 g of
activated charcoal. The hot solution was filtered and concentrated to give 5.25 g of tert-
butyl 2-[2-(lH-imidazo[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate as a light yellow syrup.
Part B
A solution of/er/-butyl 2-[2-(lH-imidazo[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate (5.25 g, 14.7 mmol) in 200 mL of CH2CI2 was treated with MCPBA (77%, 3.63 g, 16.3 mmol). After stirring overnight, the reaction mixture was treated with saturated NaHC03 solution and the layers were separated. The organic portion was washed with H2O and brine then dried over Na2S04 and concentrated to give
4.60 g of tert-butyl 2-[2-(5'Oxido-lH-imidazo[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate as a light brown foam.

Part C
A solution of tert-butyl 2-[2-(5-oxido-lH-imidazo[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate (4.60 g, 12.4 mmol) in 150 mL of 1,2-dichloroethane was heated to 80 °C and treated with 10 mL of concentrated NH4OH solution. To the rapidly stirred solution was added solid p-toluenesulfonyl chloride (2.71 g, 14.2 mmol) over a 10 min period. The reaction mixture was treated with an additional 2 mL of concentrated NH4OH solution and then sealed in a pressure vessel and heating was continued for 3 h. The reaction mixture was then cooled and treated with 100 mL of CH2CI2- The reaction mixture was then washed with H2O, 1% Na2C03 solution (3X) and brine. The organic portion was dried over Na2S04 and concentrated to give 4.56 g of tert-bntyl 2-[2-(4-amino-lH-imidazo[4,5-c]quinolin~l-yl)ethoxy]ethylcarbamate as a light brown foam, Part D
Tert-butyl 2-[2-(4-amino-lH-imida2o[4,5-c]quinolin-l-yl)ethoxy]ethylcarbamate (4.56 g, 12.3 mmol) was dissolved in 100 mL of EtOH and treated with 30 mL of 2M HCl in EtOH and the mixture was heated to reflux with stirring. After 3 h, the reaction mixture was concentrated to give a solid. The solid was triturated with hot EtOH (100 mL) and filtered to give the product as the hydrochloride salt. The free base was made by dissolving the hydrochloride salt in 50 mL of H2O and treating with 5 mL of concentrated NH4OH. The aqueous suspension was extracted with CH2CI2 (5 X 50 mL). The combined organic layers were dried over Na2S04 and concentrated to give 1.35 g of l-[2-(2-aminoethoxy)ethyl]-lH-imidazo[4,5-c]quinolin-4-amine as a tan powder. MS 272 (M + H)+;
'H NMR (300 MHz, CDCI3) 5 7.98 (d, J = 8.2 Hz, 1 H); 7.88 (s, 1 H); 7.84 (d, J = 8.4 Hz, 1 H); 7.54 (m, 1 H); 7.32 (m, 1 H); 5.43 (s. 2 H); 4.74 (t, J = 52 Hz, 2 H); 3.97 (t, J = 5.2 Hz,2H);3.42(t,7-5.1Hz,2H);2.78(t,J=5.1Hz,2H);1.10(brs,2H). PartE
The compounds in the table below were prepared according to the synthetic method of step (7) of Reaction Scheme II above using the following general method.
l-[2-(2-Aminoethoxy)ethyl]-lH-imidazo[4,5-c]quinolin-4-amine (20 mg) and 1-methyl-2-pyrrolidinone (5 mL) were combined in a test tube and then heated and sonicated to provide a solution. The sulfonyl chloride (1.1 eq.) was then added to the test tube. The test tube was capped and then placed on a shaker at ambient temperature for 20 hr. The

solvent was removed by vacuum centrifugation. The residue was purified by semi-preparative HPLC using the method described above. The products were verified by accurate mass and 1H NMR, The table below shows the structure of the free base and the observed accurate mass (M + H).








Example 50 N-[10-(4-amino-2-methyl-lH-imida2o[4,5-c]quinoIin-l-yl)-4,7-dioxadecyl]-
5-dimethylaminonaphthalene-l-sulfonamide

Part A
4,7-Dioxadecane-l,10-diamine (32.6 g, 0.185 mole) in acetonitrile (100 mL) was chilled in an ice bath. To this was slowly added dropwise dansyl chloride (5 g, 0.0185 mole) dissolved in acetonitrile (60 mL) over a 20 min. period. Stirring in an ice bath was

continued for 1.5 hr. The reaction mixture was poured into water (about 300 mL) and extracted with dichloromethane (2x 100 mL). The combined extracts were washed with water and dried to give an oil. The oil was purified by column chromatography (silica gel eluting with acetonitrile containing increasing amounts of ethanol) to provide 4,6 g of N-(10-amino-4,7-dioxadecyl)-5-dimethylaminonaphthaIene-1-sulfonamide as a viscous oil.
'H-NMR (500 MHz, CDCI3) 1-65 (2H, quin), 1.75 (2H, quin), 2.80 (2H, t, 6.59 Hz), 2.S7 (6H, s), 3.03 (2H, t, 6.1 Hz), 3.43 (2H, m), 3.47 (2H, m), 3.52 (2H, m), 3.59 (2H, t, 6.22 Hz), 7.18 (1H, d, J = 7.08 Hz)), 7.56 - 7.49 (overlapping multiplets, 2H), 8.24 (dd, 1H, J -1.2, 7.3 Hz), 8.31 (d, IH), 8.53(d, 1H). Part B
A solution of 2,4-dichIoro-3-nitroquinoline (2.71 g, 0.0115 mole) in toluene (100 mL) was cooled to 0 - 5°C in an ice bath, Triethylamine (1.5 g) was added in one portion. A solution of N-(10-amino-4,7-dioxadecyl)-5-dimethylaminonaphthalene-l-sulfonamide (4.6 g, 0.01159 mole) in toluene (60 mL) was added dropwise while maintaining the temperature below 10°C. The reaction was stirred at 2 - 5°C for 4 hrs and at a room temperature of 21°C overnight (18 hours). Thin layer chromatography (dichloromethane: ethanol) showed a trace of the amine starting material, but was mostly a bright yellow spot at the solvent front assumed to be the addition product. The toluene was removed by rotary evaporation to provide a viscous oil. The oil was purified by column chromatography (silica gel, dichloromethane/ethanol) to provide 2.4 g of N'[10-(2-chloro-3-nitro-4-quinolinyl)amino-4,7-dioxadecyl]-5-dimethy!aminonaphthalene-l-sulfonamide.
1H-NMR (CDCI3) 1.62 (2H, quin), 2.05 (2H, quin), 2.87 (6H, s), 3.03 (2H, m), 3.47 (4H, m), 3.55 (2H, m), 3.65 (2H, m), 3.73 (2H, t, 5.37 Hz), 5.75 (1H, t, 4.39 Hz,NH), 6.91 (1H, t, 4.76 Hz, NH), 7.15 (1H, d, 7.32 Hz), 7.30 (1H, m), 7.50 (2H, overlapping t), 7.62 (1H, m), 7.82 (1H, d, 7.44 Hz), 7.88 (1H, d, 8.54 Hz), 8.22 (1H, m), 8.29 (lH,d, 8.42), 8.52 (lH,d, 8.06 Hz). Part C
Material from Part B (2.2 g, 0.00357 mole) was dissolved in ethanol (150 mL). Catalyst (about 1 g of 5% Pt/C) was added and the mixture was hydrogenated using a Parr

apparatus for 30 minutes. Thin layer chromatography (ethyl acetate: hexane 1:1) showed that the reaction was complete. The reaction mixture was filtered to remove the catalyst and the filtrate was evaporated to provide a sticky solid which was shown by NMR to be crude N-[10-(3-amino-2-chloro-4-quinolinyl)amino-4,7-dioxadecyl]-5-dimethylaminonaphthalene-1-sulfonamide. It was used without further purification.
1H-NMR (500 MHz, CDCI3) 1.60 (2H, quin), 1,90 (2H, quin), 2.87 (6H, s, NMe.), 3.00 (2H, br s), 3.45 (4H, m), 3.53 (2H, m), 3.62 (2H. t, 5.62 Hz), 3.71 (4.30 (2H, br s, NH.), 5.85 (lH,brs,NH), 7.11 (1H, d, J = 7.32 Hz), 7,35 (1H, m), 7.44 - 7.48 (3H, m), 7.82 (llf, d), 8.18 (1H, dd, J = 1.1, 7.2 Hz), 8.30 (1H, d, 8.66 Hz), 8.49 (1H, d, 8.54 Hz). '^C-NMR (125 MHz) 28.48 (CH2), 30.08 (CH2), 41.93 (CH2), 45.27 (CH3), 45.28 (CH2), 69.75 (CH2). 69.83 (CH2), 70.08 (CH2), 70.38 (CH2), 114.99 (CH), 118.84 (CH), 120.84 (CH), 123.02 (CH), 123.50 (C), 125.67 (CH), 126.22 (CH), 128.01 (CH), 128.57 (C) 128.67 (CH), 129.22 (CH), 129.52(C), 129.74(C), 130.09 (CH), 134,72 (C), 137.17(C). 141.82(C), 142.03(C), 151.75(C). Part D
A portion (1 g, 1.708 mmol) of the material from Part C was dissolved in tetrahydrofuran (30 mL) and then cooled in an ice-bath to about 5°C. Freshly distilled acetyl chloride (0. 13 g, 1.78 mmol) was added with stirring. The yellow solid which immediately precipitated was isolated by filtration and washed with tetrahydrofuran. Standing in air gave an oily solid (possibly hygroscopic). FAB (fast atom bombardment) mass spectrum suggested that this was the desired N-[10-(3-acetamido-2-chloro-4' quinolinyI)amino-4,7-dioxadecyl]-5-dimethylaminonaphthalene-l-sulfonamide hydrochloride together with an undetermined amount of starting material. This solid was carried on to Part E without additional purification. Part E
The crude salt from Part D was dissolved in dry methanol containing 7% ammonia (20 mL). The solution was heated at 150°C in a bomb for 6 1/2 hrs. The reaction mixture was cooled and then concentrated. The residue was combined with acetone. Undissolved material was removed by filtration. The filtrate was concentrated and the residue was purified by column chromatography (silica gel; ethanol/dichloromethane) to provide 0.45 g ofa brown oil.

1H-NMR (400 MHz, CDCI3) 1.53 (2H, t, 7.08 Hz), 2.05 (2H, m), 2.45 (3H, s), 2.70 (611, s), 2.90 (2H, t, 5.98 Hz), 3.30 (8H, br, m), 4.40 (2H, t, 6.59 Hz), 5.75 (2H, br s NH2), 6.65 (1H, br s NHS02),7.00 (1H, d, 7.54 Hz), 7,14 (1H. t, 8.06 Hz), 7.30 (3H, m), 7.64 (!H, d, 8.30 Hz), 7.88 (1H, d, 8.18 Hz), 8.06 (1H, d, 7.33 Hz), 8.24 (1H, d, 8.54 Hz), 8,36 (1H, d, 8.55 Hz).
This material was then purified by high performance liquid chromatography using a Bondapak CI8 12.5 nm reverse phase column (available from Waters, Milford, MA) eluting with a composite gradient of acetonitrile in water to provide the desired product.
CYTOKINE INDUCTION IN HUMAN CELLS
An in vitro human blood cell system is used to assess cytokine induction. Activity is based on the measurement of interferon and tumor necrosis factor (a) (IFN and TNF, respectively) secreted into culture media as described by Testerman et. al. In 'Cytokine Induction by the Immunomodulators Imiquimod and S-27609", Journal of Leukocyte Biology, 58, 365-372 (September, 1995). Blood Cell Preparation for Culture
Whole blood from healthy human donors is collected by venipuncture into EDTA vacutainer tubes. Peripheral blood mononuclear cells (PBMCs) are separated from whole blood by density gradient centrifugation using Histopaque®-1077. The PBMCs are washed twice with Hank's Balanced Salts Solution and then are suspended at 3-4 x 106 cells/mL in RPMI complete. The PBMC suspension is added to 48 well flat bottom sterile tissue culture plates (Costar, Cambridge, MA or Becton Dickinson Labware, Lincoln Park, NJ) containing an equal volume of RPMI complete media containing test compound. Compound Preparation
The compounds are solubilized in dimethyl sulfoxide (DMSO). The DMSO concentration should not exceed a final concentration of 1% for addition to the culture wells. Incubation
The solution of test compound is added to the first well containing RPMI complete and serial dilutions are made in the wells. The PBMC suspension is then added to the

wells in an equal volume, bringing the test compound concentrations to the desired range. The final concentration of PBMC suspension is 1.5-2 X 106 cells/mL. The plates are covered with sterile plastic lids, mixed gently and then incubated for 18 to 24 hours at 37°C in a 5% carbon dioxide atmosphere. Separation
Following incubation the plates are centrif uged for 5-10 minutes at 1000 rpm (--200 X g) at 4°C. The cell-free culture supernatant is removed with a sterile polypropylene pipet and transferred to sterile polypropylene tubes. Samples are maintained at -30 to -70°C until analysis. The samples are analyzed for interferon-a and for tumor necrosis factor-a by ELISA* Interferon (a) and Tumor Necrosis Factor (a) Analysis by ELISA
Interferon-a concentration is determined by ELISA using a Human Multi-Species kit from PBL Biomedical Laboratories, New Brunswick, NJ. Results are expressed in pg/mL.
Tumor necrosis factor-a concentration is determined using ELISA kits available from Genzyme, Cambridge, MA; R&D Systems, Minneapolis, MN; or Pharmingen, San Diego, CA- Results are expressed in pg/mL.
The table below lists the lowest concentration found to induce interferon and the lowest concentration found to induce tumor necrosis factor for each compound. A '*" indicates that no induction was seen at any of the tested concentrations; generally the highest concentration tested was 10 or 30 µM*








WHAT IS CLAIMED IS:
I. A compound of the formula (I):

(I)
wherein: X is -CHR5-, -CHRs-alkyl-, or -CHRj-alkenyl-;
R1 is selected from the group consisting of: -R4-NR3-S02-R6-alkyl; -R4—NR3—S02-R6-alkenyl; -R4-NR3~S02-R6-aryl; -R4-NR3-S02-R6-heteroaryl; -R4-NR3-S02-R6-heterocyclyl;
-R4-NR3-SO2-R7;
-R4-NR3-S02-NR5-R6-aIkyl;
-R4-NR3-S02-NR5-R6-alkenyl;
-R4-NR3-S02-NR5-R6-aryl;
-R4-NR3-S02-NR5-R6-heteroaryl;
-R4-NR3-S02-NR5-R6-heterocyclyl; and
-R4-NR3-SO2-NH2;
R2 is selected from the group consisting of: -hydrogen; -alkyl; -alkenyl;

-aryl;
-heteroaryl;
-heterocyclyl;
-alkyl-Y-alkyl;
-alkyl-Y-alkenyl;
-alkyl-Y-aryl; and
-alkyl or alkenyl substituted by one or more substment selected
from the group consisting of; -OH; -halogen; -N(R5)2; -CO-N(R5)2; -CO-CMO alkyl; -CO-O-CMO alkyl;
-N3;
-aryl;
-heteroaryl;
-heterocyclyl;
-CO-aryl; and
-CO-heteroaryl;
Y is -O" or -S(0)o.2-;
R3 is H, CMO alkyl, or arylalkyl;
each R4 is independently alkyl or alkenyl, which may he interrupled by one
or more -O- groups; or R3 and R4 can join together to form a ring ; '
each R5 is independently H, CMO alkyl, or C2-10 alkenyl;
6 is a bond, alkyl, or alkenyl, which may be interrupted by one or more
"O- groups;
R7 is C1-10 alkyl; or R3 and R7 can join together to form a rmi'.:
n is 0 to 4; and
each R present is independently selected from the group consisting of C1-10
alkyl, C1-1 alkoxy, hydroxy, halogen and trifluoromethyl.

or a pharmaceutically acceptable salt thereof.
2. A compound or salt of claim 1 wherein X is -CH(alkyl){alkj \i , A ';.■:.MT. \\:- alkyl groups can be the same or different,
3. A compound or salt of claim 1 wherein X is -CH2-CH2-.
4. A compound or salt of claim 1 wherein X is -CH(C2H5)(CH •)
5. A compound or salt of claim 1 wherein R2 is H.
6. A compound or salt of claim 1 wherein R2 is alkyl.
7. A compound or salt of claim 1 wherein R2 is -alkyl-0-alk\'l.
8. A compound or salt of claim 1 wherein R3 and Rejoin to form a hetermeyclic ring.
9. A compound or salt of claim 1 wherein R1 is -R4-NR3-SO: R aryl
10. A compound selected from the group consisting of: N-(2-{2-[4-amino-2'(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-1-
yl]ethoxy}ethyl)methanesulfonamide; N-(2-{2-[4-aminO'2-(2-methoxyethyl)-6,7,8,9-tetrahydro-lH-imida/(4,5-quinolin-1-
yl]ethoxy}ethyl)methanesulfonamide;
N-(2-{2-[4-amino-2'(2-methoxyethyl)-lH-imidazo[4,5-c]quinolin-l-yl]ethxyrthyl-N--
methylmethanesulfonamide;
N-(2-{2-[4-amino-2-(2-methoxyethyl)'6,7,8,9-tetrahydro-lH-imidazo]4,5-equinolin-1-yl]ethoxyjethyl)-N-methylmethanesulfonamide; 2-butyl-1 - {2-[2-( 1,1 -dioxidoisothiazolidin-2-yl)ethoxy]ethyl} -lH-imidazo[4,5-c]quinolin-4-amine;and
N-[10-(4-amino-2-methyl-lH-imidazo[4,5-c]quinolin-l-yl)-4,7-dioxatdeeu;]-5-dimethylaminonaphthalene-1-sulfonamide;

or a pharmaceutically acceptable salt thereof.

wherein: X is -CHR5-, -CHRs-alkyl-, or -CHRs-alkenyl-;
R1 is selected from the group consisting of; -R4-NR3-S02-R6-alkyl; -R4—NR3-SO2—R6-alkenyl; -R4-NR3-S02-R6-aryl; -R4-NR3-S02-R6-heteroary 1; -R4-NR3-S02-R6-heterocycly!;
-R4—NR3-SO2-R7; -R4-NR3-S02-NRs-R6-alkyl; -R4-NR3-S02-NR5-R6-alkenyl; -R4-NR3-S02-NR5-R6-aryl; -R4-NR3-S02-NR5-R6-heteroaryl; -R4-NR3-S02-NR5-R6-heterocyclyl; and
-R4-NR3-SO2-NH2; 1
R2 is selected from the group consisting of: -hydrogen; -alkyl; -alkenyl; -aryl;
-heteroaryl; -heterocyclyl;

-alkyl-Y-alkyl;
-alkyl-Y-alkenyl;
-alkyl-Y-aryl; and
- alkyl or alkenyl substituted by one or more substituents selected
from the group consisting of:
-OH;
-halogen;
-N(R5)2;
-CO-N(R5)2; -CO-CMO alkyl;
-CO-O'CMO alkyl;
-N3;
-aryl;
-heteroaryl;
-heterocyclyl;
-CO-aryl; and
-CO-heteroaryl;
Y is -O- or -S(0)o,2-;
R3 is H, C1-10 alkyl, or alylalkyl;
each R4 is independently alkyl or alkenyl, which may be interrupted by one
or more -O- groups; or R3 and R4 can join together to form a ring;
each R5 is independently H, C1-10 alkyl, or C2-10 alkenyl;
R6 is a bond, alkyl, or alkenyl, which may be interrupted by one or more
-O- groups; '
R7 is C1-10 alkyl; or R3 and R7 can join together to form a ring;
n is 0 to 4; and
each R present is independently selected from the group consisting of CMO
alkyl, C1-10 alkoxy, hydroxy, halogen, and trifluoromethyl;
or a pharmaceutically acceptable salt thereof.
I. A compound or salt of claim 11 wherein R2 is H or alkyl.

13. A compound or salt of claim 11 wherein R2 is -alkyl-0-alkyl.
14. A pharmaceutical composition comprising a therapeutically effective amount of a
compound or salt of claim 1 and a pharmaceutically acceptable carrier.
15. A method of inducing cytokine biosynthesis in an animal comprising administering
a therapeutically effective amount of a compound or salt of claim 1 to the animal.
16. The method of claim 15 wherein the cytokine is IFN-a.
17. A method of treating a viral disease in an animal comprising administering a
therapeutically effective amount of a compound or salt of claim 1 to the animal.
18. A method of treating a neoplastic disease in an animal comprising administering a
therapeutically effective amount of a compound or salt of claim 1 to the animal.
19. A compound of the formula (III):


-R4-NR3-S02-R6-heteroaryl; -R4-NR3-S02-R6-heterocyclyl;
-[14-NR3-S02-R7; -R4-NR3-S02-NR5-R6-alky];
-R4-NR3-S02-NR5-R6-alkenyl;
-R4-NR3-S02-NR5-R6-aryl;
-R4-NR3-S02-NR5-R6-heteroaryl;
-R4-NR3-S02-NR5-R6-heterocyclyl;and
-R4-NR3-SO2-NH2;
R2 is selected from the group consisting of:
-hydrogen;
-alkyl;
-alkenyl;
-aryl;
-heteroaryl;
-heterocyclyl;
-alkyl-Y-alkyl;
-alkyl-Y- alkenyl;
-alkyl-Y-aryl; and
- alkyl or alkenyl substituted by one or more substituents selected
from the group consisting of:
-OH;
-halogen;
-N(R5)2;
-CO-N(R5)2;
-CO-C1-10 alkyl;
-CO-O-C1-10 alkyl;
-N3;
-aryl;
-heteroaryl;
-heterocyclyl;
-CO-aryl; and

-CO-heteroaryl;
Y is -0- or -S(0)o-2-;
R3 is H, C1-10 alkyl, or arylalkyl;
each R4 is independently alkyl or alkenyl, which may be interrupted by one
or more -O- groups; or R4 and R3 can join to form a ring;
each R5 is independently H, C1-10 alkyl, or C2-10 alkenyl;
R6 is a bond, or is alkyl or alkenyl, which may be interrupted by one or
more -0- groups;
R7 is C1-10 alkyl; or R3 and R7 can join together to form a ring;
n is 0 to 4; and
each R present is independently selected from the group consisting of CMO
alkyl, Ci-10 alkoxy, hydroxy, halogen and trifluoromethyl;
or a pharmaceutically acceptable salt thereof.
20. A pharmaceutical composition comprising a therapeutically effective amount of a compound or salt of claim 11 and a pharmaceutically acceptable carrier.
21. A method of inducing cytokine biosynthesis in an animal comprising administering a therapeutically effective amount of a compound or salt of claim 11 to the animal.
22. The method of claim 21 wherein the cytokine is IFN-a.
23. A method of treating a viral disease in an animal comprising administering a therapeutically effective amount of a compound or salt of claim 11 to the animal.
24. A method of treating a neoplastic disease in an animal comprising administering a therapeutically effective amount of a compound or salt of claim 11 to the animal

25, A compound substantially as herein described and exemplified.
26. A method of treating a neoplastic disease in an animal substantially
as herein described and exemplified.


Documents:

892-chenp-2003-abstract.pdf

892-chenp-2003-claims filed.pdf

892-chenp-2003-claims granted.pdf

892-chenp-2003-correspondnece-others.pdf

892-chenp-2003-correspondnece-po.pdf

892-chenp-2003-description(complete)filed.pdf

892-chenp-2003-description(complete)granted.pdf

892-chenp-2003-form 1.pdf

892-chenp-2003-form 26.pdf

892-chenp-2003-form 3.pdf

892-chenp-2003-form 5.pdf

892-chenp-2003-other documents.pdf

892-chenp-2003-pct.pdf


Patent Number 212210
Indian Patent Application Number 892/CHENP/2003
PG Journal Number 02/2008
Publication Date 11-Jan-2008
Grant Date 26-Nov-2007
Date of Filing 06-Jun-2003
Name of Patentee M/S. 3M INNOVATIVE PROPERTIES COMPANY
Applicant Address 3M Center, P.O. Box 33427, Saint Paul, MINNESOTA 55133-3427
Inventors:
# Inventor's Name Inventor's Address
1 CROOKS, Stephen, L 82 Pine Street, Mahtomedi, MN 55115-1937
PCT International Classification Number C07D 471/04
PCT International Application Number PCT/US2001/046582
PCT International Filing date 2001-12-06
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 60/254,218 2000-12-08 U.S.A.