Title of Invention

A COMPOSITION FOR CONTROLLING INSECTS AND A PROCESS FOR PREPARING THE SAME

Abstract A composition for controlling insects and a process for preparing the same This invention relates to a composition for controlling insects particularly the mosquito bug. It consists of a blend of (E)"2-hexen-l-ol and (Z)-3-hexenyl acetate preferably in the ratio of 1:3 to 1: 7 volume/volume. This invention also includes a process for the preparation of the above composition wherein the two components are well blended preferably in the ratio stated above. A bait for attracting male tea bugs mosquito having a blend of the two compounds is also part of this invention. The composition exhibits species specific attraction towards male tea mosquito bugs.
Full Text

This invention relates to a composition for controlling insects and a process for preparing the same. More particularly, this invention relates a composition for controlling tea mosquito bugs and its preparation.
The present day trend is to discourage the use of chemical pesticides for control and irradiation of pests to avoid long-term adverse effect on the environment, resistance to a particular chemical due to prolonged use, cumulative and non-cumulative side effects, that surface due to the use of pesticide contaminated products are some of the main reasons against the continuous use of chemical pesticides. Biological, eco and environmentally friendly measures for controlling pests assume significance under this circumstance.
Pheromones are chemical substances produced by animals and insects. They function as stimuli to other individual of the same species for one or more behavioral responses. Sex pheromones of some insects have been used for trapping insects of the opposite sex for bio assay.
An object of this invention is to control tea mosquito bugs by luring adult males with the sex pheromones of female tea mosquito bugs. Yet another object is to develop a chemical composition similar to the volatiles secreted by female tea mosquito bugs which shows optimum attraction to the males of the same species.
Studies were conducted to identify the sex-attracted pheromones produced by female tea bugs mosquito, scientifically known as Helopeltis theilvora, Volatiles produced by newly emerged unmated females were

extracted and analysed by gas chromatography and identified by mass spectrum. From important compounds, among them, two more predominant compounds were identified. Studies were carried out to assess the response of male mosquito to these compounds. Males were found to be highly sensitive in their behavioral response to these compounds. Sensitivity and orientation towards the source of pheromone were also found to be optimum. It was also observed that a combination of two specific compounds exhibited maximum potential for attracting the males of the species.
Studies were then conducted with the identified chemical compounds alone and in combination with each other. Volatile extracts containing species specific pheromones secreted by female tea bugs are found to contain trans-2-hexenyl acetate, cis-3-hexen-l-ol (Z)-3-hexenyl acetate and (E)-2-hexen-l-ol.
Commercially available cis-3-hexen-ol was converted into (E)-2-hexen-l-ol by acetylating with acetic acid and pyridine in the presence of dichloromethane trans-2-hexenyl acetate was deacetylated using sodium carbonate in the presence of methanol and converted into (Z)-3-hexenyl acetate.
These compounds were used as attractants for male tea mosquito bugs individually and in blends. Our study showed that individual compounds did not exhibit any species specific attraction towards female mosquito bugs. However blends of (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate in proportions varying from 1 : 3 to 1: 7 showed high attraction rate.
Data of field attraction studies of two different combinations is given below :


Degree of attraction corresponding to + + is medium; corresponding to + + + is high and corresponding to + + + + is very high.
These compositions of synthetic chemicals can replace natural pheromones in effectively trapping of tea mosquito bugs, which are responsible for considerable damage in tea estates. Trapping and subsequent elimination of male mosquito bugs will adequately control and eventually even eradicate the mosquito population. Any conventional means of trapping and destruction may be used for this purpose. Strips and baits provided with the blend of the two compounds or tunnels having the bait dispersed therein may be used for this purpose.
Our studies clearly showed that a composition containing (E)-2-hexen-l-ol and (Z)-3"hexenyl acetate preferably in the ratio of 1:3 to 1: 7 show

synergistic properties and that the two compounds when individually used failed to attract male tea bug mosquitoes.
Accordingly this invention relates to a composition for controlling insects such as Helopeltis theivora or tea mosquito bug which comprises a blend of (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate.
This invention also relates to a process for preparing a composition for controlling Helopeltis theivora or tea mosquito bug, which comprises the step of blending (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate.
Attraction studies and experiments were carried out to show the effectivity and specificity of the compositions. Rubber Septa baited with the composition and blank baits were set up for such studies, which were carried out at many locations. The results obtained clearly showed that the composition of this invention exhibited medium to very high attraction towards male tea bug mosquitoes.
Control and elimination male mosquito bugs eventually eradicates tea mosquito bugs, which cause considerable damage in tea estates.
The above disclosure and the appended claims do not exclude obvious alteration and modification known to persons skilled in the art.



WE CLAIM :
1. A composition for controlling insects such as Helopeltis theivora or tea mosquito bug comprising a blend of (E)-2-hexen-1 -ol and (Z)-3-hexenyl acetate.
2. The composition as claimed in claim 1, wherein (E)-2-hexen-l-ol and (Z)-3-hexenylacetate are present in the range of 1:3 to 1:7 volume/volume.
3. The composition as claimed in claim 2, wherein the ratio of (E)-2-hexen-ol and (Z)-3-hexenyl acetate is 1:7.
4. The composition as claimed in claims 2 or 3, wherein said (E)-2-hexen-l-ol is prepared by converting cis-3-hexene-l-ol to (E)-2-hexen-l-ol by acetylating acetic acid and pyridine in the presence of dichloromethane.
5. The composition as claimed in claims 2 or 3, wherein said (Z)-3-hexenyl acetate is prepared by deacetylating trans-2-hexenyl acetate with sodium carbonate in the presence of methanol.
6. A process for preparing a composition for controlling Helopeltis theivora or tea mosquito bug comprising the step of blending (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate.
7. The process as claimed in claim 6, wherein said (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate are blended in the ratio of 1:3 to 1:7 volume/volume.

8. The process as claimed in claim 2, wherein said (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate are blended in the ratio of 1:7 volume/volume.
9. The process as claimed in claims 6 to 8, wherein said (E)-2-hexen-l-ol is prepared by acetylating cis-3-hexene-l-ol with acetic acid and pyridine in the presence of dichloromethane.
10. The process as claimed in claims 6 to 8, wherein said (Z)-3-hexenyl acetate is prepared by deacetylating trans-2-hexenyl acetate with sodium carbonate in the presence of methanol.
11. A bait for attracting insects such as tea mosquito bug having a blend of (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate.
12. The bait as claimed in claim 11, wherein said blend of (E)-2-hexen-l-ol and (Z)-3-hexenyl acetate is in the ratio of 1:3 to 1:7.
13. A composition for controlling insects such as Helopeltis theivora or tea mosquito bug substantially as herein described.
14. A process for preparing a composition for controlling Helopeltis theivora or tea mosquito bug, substantially as herein described.

15. A bait for attracting insects such as tea mosquito bug, substantially as herein described.


Documents:

575-che-2003-abstract.pdf

575-che-2003-claims duplicate.pdf

575-che-2003-claims original.pdf

575-che-2003-correspondnece-others.pdf

575-che-2003-correspondnece-po.pdf

575-che-2003-description(complete) duplicate.pdf

575-che-2003-description(complete) original.pdf

575-che-2003-form 1.pdf

575-che-2003-form 18.pdf

575-che-2003-form 26.pdf

575-che-2003-form 3.pdf


Patent Number 209074
Indian Patent Application Number 575/CHE/2003
PG Journal Number 38/2007
Publication Date 21-Sep-2007
Grant Date 20-Aug-2007
Date of Filing 15-Jul-2003
Name of Patentee M/S. UPASI TEA RESEARCH FOUNDATION
Applicant Address TEA RESEARCH INSTITUTE, NIRAR DARN BPO, VALPARAI 642 127,
Inventors:
# Inventor's Name Inventor's Address
1 NARAYANAN NAIR MURALEEDHARAN TEA RESEARCH INSTITUTE, NIRAR DAM BPO, VALPARAI 642 127,
2 RAJAGOPAL SELVASUNDARAM UPASI TEA RESEARCH FOUNDATION TEA RESEARCH INSTITUTE, NIRAR DAM BPO, VALPARAI 642 127,
PCT International Classification Number A01N 25/00
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA