Indian Patents. 208190:PROCESS FOR THE CONVERSION OF THREO (2R,3R)-2-HYDROXY-3(2-AMINOPHENYLTHIO)-3-(4-METHOXY PHENYL) PROPIONIC ACID DERIVATIVES INTO MIXTURES OF ENANTIOMERS

Title of Invention	PROCESS FOR THE CONVERSION OF THREO (2R,3R)-2-HYDROXY-3(2-AMINOPHENYLTHIO)-3-(4-METHOXY PHENYL) PROPIONIC ACID DERIVATIVES INTO MIXTURES OF ENANTIOMERS
Abstract	A process which allows the re-use of compounds of formula in diltiazem synthesis through a process of conversion to a mixture of enamiomers III-(2R,3R) and III- (2S.3S) is described.

Full Text	The following Specification Particularly describes and ascertains the nature of this invention and the manner in which it is to be performed The present invention relates to a method for the preparation of thero-2-hydroxy-3-(2-aminophenylthio-3-(4-methoxyphenyl) propionic acid esters, intermediates useful for the synthesis of diltiazem, starting from (2R,3R)-2-hydroxy-3-(2-aminophenylghio)-3-(4-methoxyphenyl) propionic acid or derivatives thereof. The invention also relates to preparation of Dilitazem from the above proponic acid ester prepared by the method of this inventions. The method of the present invention permits recycle of a wate product of a conventional Diltiazem Synthesis. Diltiazem, (+)-(2S/3S)-3-acetoxy-5-[2-(dimethylamino)-2-dihydro-2(-4-methoxy-phenyl)-1,5-benzothiazepin-4(5H)-one (The Merck Index, XII ed., no. 3247, page541) is a known drug with calcium-antagonist activity described in the U.K. patent 1236467 (Tanabe Seiyaku Co. Ltd.). Several methods for the preparation of diltiazem such as, for example, those described in the above cited U.K. patent 1236467, in the European patent application 0 059 335 and in the Japanese patent no. 71/8982, all in the name of Tanabe Seiyaku Co. Ltd., are described in the literature. Most of these methods substantially provide for the following synthetic scheme. Scheme 1 -1- wherein R is a hydrogen atom or a lower alkyl and the asterisks mark the stereogenic carbon atoms. These methods use the compound of formula III-threo in racemic form, as intermediate. 8 However, diltiazem shows stereocenters with S configuration and then, following the above reported scheme, a step for the separation of the (2S,3S) enantiomer from the (2R,3R) one is necessary. The separation of the two enantiomers can be carried out on the intermediate (III) in the form of an ester (R=alkyl) as well as in the form of acid (R=H). 10 For example, the separation of the (2S,3S) and (2R,3R) enantiomers at the level of the ester III can be carried out by using an optically active acid as resolving agent (U.S. patent 5,144,025 - Zambon Group S.p.A.) or by spontaneous resolution (U.S. patent 5,097,059 - Zambon Group S.p.A.). In case of separation at the level of the acid III, the methods described in the literature 15 provide for, inter alia, the resolution with optically active bases such as a-phenylethylamine (European patent application 0 098 892 - Tanabe Seiyaku Co. Ltd) or L-lysine (U.K. patent application 2130578 - Istituto Luso Farmaco d'ltalia S.p.A.) or the acylation in the presence of a lipase (European patent application 0 617 130 - Orion-Yhtyma Oy Fermion). It is evident that the resolution methods have the disadvantage of giving the desired (2S,3S) 20 isomer with a maximum theoretical yield of 50% on the raceme and of giving also the corresponding (2R,3R) isomer, at the same time. The isomers with (2R,3R) configuration, unsuitable for diltiazem synthesis, are then a waste product in the industrial synthesis. As a consequence, a process for the recycle of said compounds in order to recover them for 25 diltiazem synthesis should be useful. As far as we know, the only method described in the literature for the recycle of an intermediate with (2R,3R) configuration is the process claimed in the U.S. patent 5,102,999 (Zambon Group S.p.A.) which provides for the racemization of the intermediate IV-cis (2R,3R). -3- We have now found a process for the conversion of the intermediates 1II-(2R,3R) to a mixture of enantiomers III-(2R,3R) and III-(2S,3S) which allows to re-use the enantiomers with (2R,3R) configuration of the intermediates of formula III for diltiazem synthesis. Therefore, object of the present invention is a process for the conversion of thero-(2R,3R)-2-hydroxy-3-(2-aminophenylthio)-3-(4-methoxypbenyl)propionic acid derivatives of formula 10 ,OCH3 III-(2R,3R) wherein R1 is a linear or branched C1-C3 alkyl or a hydrogen atom; into a mixture of enantiomers III-(2R,3R) and III-(2S,3S), characterised by the following steps: (a) the cyclization of the compound III-(2R,3R) to afford the corresponding compound of formula 20 I0CH3 1V-(2R,3R) 75 30 (b) the conversion to the compound of formula wherein R2 is a hydrogen alkyl or a C3Cacyl group, (C) the reduction to afford the compound of formula (d) the opening reaction by treating with a strong acid or with a strong base in an alcoholic or aqueous solvent. The mixture of the two enantiomers III-(2R,3R) and III-(2S,3S) obtained with the process object of the present invention can be resolved according to the already reported methods in order to separate the (2S, 3S) enantiomer and if desired, preparing Diltiazem from the said enantiomer. In the present context, if not otherwise specified, the term mixture of enantiomers means a substantially racemic mixture (2R,3R:2S,3S ratio about 1:1) or a mixture wherein the enantiomer 2S,3S prevails. Analogously, if the absolute configuration of the compounds of formula III or IV is not indicated, it means that said compounds are a substantially racemic mixture (2R,3R:2S,3S ratio about 1:1) or a mixture wherein the enantiomer 2S, 3S prevails. The term liner or branched C1-C3 alkyl means methyl, ethyl, propyl, isopropyl and the term C2-C4 acyl group means acetyl, propionyl, butyryl, isobutyryl. The intermediates of formula III-(2R,3R) used as starting products in the process object of the present invention are known compounds and they are obtained as waste products in the optical separation processes for diltiazem synthesis. Generally the compounds of formula III are in the form of acid (R1=H) or in the form of methyl (Rl=CH3) or ethyl (R1-CH2-CH3) ester. Preferably, in the process object of the present invention the compounds of formula III are methyl esters. The cyclization of the compound III-(2R,3R) to afford the compound of formula IV-(2R,3R) can be carried out according to the known methods for the cyclization of the corresponding (2S,3S) enantiomer. For example, the cyclization of the esters of formula I1I-(2R,3R) can be carried out by treating with fosfonic acids (U.S. patent 5,223,612 - Zambon Group S.p.A.) or by treating with sulfonic acids (European patent application 0 447 135 - Tanabe Seiyaku Co. Ltd.). Similarly, the cyclization of the acid of formula III-(2R,3R) can be carried out by treating with sulfonic acids (European patent application 0 395 323 - Tanabe Seiyaku Co. Ltd.) or with bases (European patent application 0 450 705 - Stamicarbon B.V.). In the process object of the present invention the cyclization reaction is preferably carried out starting from the methyl ester of formula II1-(2R,3R) by treating with cis-propenyl- fosfonic acid. The subsequent conversion reaction to the derivative V can be carried out according to known methods too. For example, the methods described in J. Org. Chem., 1996, 8586-8590 or in the already cited U.S. patent 5,102,999 can be used. Preferably, in the process object of the present invention, the acetyl derivative of formula V (R2=COCH3) is prepared, then hydrolysed to afford the compound Va which will be in equilibrium with its tautomer (Vb) as herein below reported. 30 ,OCH3 .OCH, Va Vb The subsequent reduction reaction allows to obtain the compound IV-cis. Optionally, the hydrolysis and reduction reactions can be carried out one-pot, that is in an unique reaction environment, without isolating the compound Va (or Vb). The preparation of the acetyl derivative V is preferably carried out by treating with acetic anhydride in dimethylsulfoxide, in the presence of catalytic amounts of pyridine. The optional hydrolysis can be then carried out by treating with bases such as sodium hydroxide or sodium mesylate. The reduction of the compound of formula V can be carried out with known methods, for example by treating with hydrides according to what reported in the already cited U.S. patent 5,102,999. The resultant compound IV-cis can be then converted into the corresponding compound of formula III-threo by treating with a strong acid or with a strong base in an alcoholic or aqueous solvent. The amount of acid or base is at least equimolar, preferably in excess, with respect to the compound IV-cis. Generally the reaction is carried out by using an excess of acid or base equal to 10%-30% in moles with respect to the compound IV. The strong acids used in the process of the invention are inorganic acids such as hydrochloric, hydrobromic, sulfuric and fosforic acid or organic acids such as sulfonic acids, preferably methanesulfonic,p-toluenesulfonic and camphorsulfonic acid. Sodium hydroxide is preferably used as strong base. -7- In the process object of the present invention methanesulfonic acid is preferably used. The reaction is carried out in an alcoholic solvent such as, for example, methanol or ethanol, preferably in methanol, or in water optionally in admixture with a suitable co-solvent such as dimethylsulfoxide. Dependently on the alcohol used as solvent the corresponding ester of formula III-threo is obtained which can then be used again in the preparation of diltiazem according to the synthetic route illustrated in the previous scheme 1. When an aqueous solvent is used, the acid (R1=H) of formula III-threo is obtained and used according to the synthetic route illustrated in scheme 1 too. The opening reaction of the compound of formula IV-cis represents the most characterising feature of the process object of the present invention. In fact, as far as we know, this reaction has never been described in the literature. On the contrary, cyclizations of the compound III-threo to afford the compound IV-cis by using acids or bases have widely been described in the literature. Furthermore, it is evident that the possibility of racemizing the waste products with (2R,3R) configuration of the process for diltiazem preparation at the level of one of the earliest synthetic intermediates represents a relevant advantage from a practical and economic viewpoint. 20 A particularly preferred embodiment of the process object of the present invention is the following. The methyl ester of formula III-(2R,3R), obtained by resolution of the corresponding racemic mixture, is cyclized with cis-propenyl-fosfonic acid and then oxidised by treatment with acetic anhydride/dimethylsulfoxide/pyridine obtaining the acetyl derivative of formula V. After basic hydrolysis and reduction with sodium borohydride the racemic compound IV- cis is obtained and treated with an excess of methanesulfonic acid in methanol up to the obtainment of the racemic methyl ester III-threo. The resolution of the racemic methyl ester III-threo allows to obtain the compound III- (2S,3S) which is used for diltiazem synthesis and the enantiomer III-(2R,3R) which can undergo a further recycle phase according to the process object of the present invention. ¦8- In order to better illustrate the present invention the following examples are now given. Example 1 A mixture of methyl (2R,3R)-2-hydroxy-3-(2-aminophenylthio)-3-(4-metrioxyphenyl)- propionate (5 g; 15 mmoles) and cis-propenyl-fosfonic acid (0.183 g; 1.5 mmoles) in xylene (35 ml) was heated under reflux and stirring for 5.5 hours. After distilling a xylene/methanol mixture (about 3%), the reaction mixture was cooled to 15°C. The resultant precipitate was filtered under vacuum, washed with xylene (2x5 ml) and then dried in oven at 65°C obtaining (2R,3R)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-l,5- benzothiazepin-4(5H)-one (42 g; 89.6% yield). Example 2 A catalytic amount of pyridine (19.7 g; 0.25 moles) was added to a solution of (2R,3R)-2,3- dihydro-3-hydroxy-2-(4-methoxyphenyl)-l,5-benzotbiazepin-4(5H)-one (500 g; 1.66 moles) in dimethylsulfoxide (1100 g; 14.1 moles) and acetic anhydride (425 g; 4.17 moles). After keeping the solution under stirring at room temperature for 24 hours, water (1000 ml) was slowly added and the mixture was kept under stirring for 30 minutes. The crystalline precipitate was filtered off, washed with methanol and dried obtaining 3- acetoxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (481.6 g; 84.7% yield). Example 3 A suspension of 3-acetoxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (17.1 g; 0.05 mmoles) in methanol (51 ml) was cooled in ice and then a solution of NaOH (5 g; 0.125 mmoles) in water (63 ml) was added. The solution was then kept under stirring for 2 hours at room temperature. After neutralisation with HCL 2N (50 ml) and extraction with ethyl acetate, the organic phase was washed twice with water, dried and concentrated obtaining a viscous oil. The oil was treated with ethyl ether obtaining 2-(4-methoxyphenyl)-l,5-benzothiazepin- 3,4(2H,5H)-dione (12.98 g; 86.7% yield) as a crystalline solid. Example 4 Sodium borohydride (0.567 g; 15 mmoles) was added to a solution of 2-(4-methoxyphenyl)-1,5-benzothiazepin-3,4(2H,5H)-dione (4.25 g; 14.2 mmoles) in methanol (65 ml), kept under stirring, at 15°C. After 1 hour the reaction mixture was poured into a buffer solution (100 ml) at pH 7 and methanol was removed by distillation under vacuum. The resultant mixture was extracted with methylene chloride (2x30 ml). After evaporation of the solvent under reduced pressure cis-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (4.15 g; 97% yield) as racemic mixture was obtained. Example 5 In a 500 ml flask, equipped with thermometer and condenser, cis-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one (50 g; 165.9 mmoles) was suspended in methanol (200 ml) and methanesulfonic acid (19.2 g; 200 mmoles) was added to the mixture. The reaction mixture was brought to reflux and the progress of the reaction was followed by TLC (eluent ethylacetate:hexane=6:4). After heating for 7 hours, the content of starting product was about 1%. The reaction mixture was then cooled at room temperature before adding, with a dropping funnel, a 8% solution of sodium bicarbonate (208 g; final pH=7.0). The resultant precipitate was filtered off and washed three times with water (3x50 ml). The resultant solid was then dried at 50°C under vacuum up to constant weight obtaining methyl threo-2-hydroxy-3-(2-aminophenylthio)-3-(4-inethoxyphenyl)-propionate (54 g; molar yield 94.2%; HPLC titre 96.5%). Example 6 30% Sodium methylate in methanol (0.1 ml; 0.5 mmoles) and, after 15 minutes, sodium borohydride (54 mg; 1.42 mmoles) were added to a suspension of 3-acetoxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (1 g; 2.9 mmoles) in methanol (5 ml). After keeping the mixture under stirring at room temperature for 5 hours, methanesulfonic acid (0.6 ml; 9.3 mmoles) was added and the mixture was heated under reflux for 5 hours. - After cooling at room temperature, 8% sodium bicarbonate (9 ml) was added. After adding toluene, the resultant solid was filtered off, washed with water (3x1 ml) and dried at 50°C under vacuum obtaining methyl threo-2-hydroxy-3-(2-aminophenylthio)-3-(4- methoxyphenyl)propionate (0.92 g; molar yield 90%; HPLC titre 95%). Example 7 30% Sodium methylate in methanol (0.5 ml; 2.5 mmoles) and, after 15 minutes, sodium borohydride (0.28 g; 7.35 mmoles) were added to a suspension of 3-acetoxy-2-(4- methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (5 g; 14.7 mmoles) in methanol (25 ml). After keeping the mixture under stirring at room temperature for 5 hours, a 6.5M solution of hydrochloric acid in methanol (2.7 ml; 17.6 mmoles) was added and the mixture was heated under reflux for 5 hours. After cooling at room temperature, 8% sodium bicarbonate (16 ml) was added. The resultant precipitate was filtered off, washed with water (3x5 ml) and dried at 50°C under vacuum obtaining methyl threo-2-hydroxy-3-(2-aminophenylthio)-3-(4- methoxyphenyl)-propionate (5 g; molar yield 85%; 1H-NMR titre 75%). We claim, 1. A process for the conversion of threo-(2R,3R)-2-hydroxy-3-(2-aminophenyltIiio)-3-(4-methoxyphenyl)propionic acid derivatives of formula uridtiiOH COOR1 .OCH, III - (2R,3R) wherein R is a linear or branched C1-C3 alkyl or a hydrogen atom; into a mixture of enantiomers III-(2R,3R) and III-(2S,3S), characterised by the following steps: (a) the cyclization of the compound III-(2R,3R) to afford the corresponding compound of formula IV-(2R,3R) wherein R2 is a hydrogen atom or a C2-C4 acyl group, ( c) the reduction of the compound of formula (V) of step (b) to afford the compound of formula OH OCH3 IV - cis (d) the opening reaction by treating with a strong acid or with a strong base in an alcoholic or aqueous solvent to get the mixture of enantiomers of formula III-(2R, 3R)and III(2S,3S) 2) A process according to claim 1 for the conversion of the methyl ester of threo (2R,3R)- 2-hydroxy -3-(2-aminophenylthio)-3-(4-methoxyphenyl)-propionic acid. 3) A process according to claim 1 wherein the compound of formula V wherein R is acetyl is used. 4) A process according to claim I wherein the opening reaction is carried out by treating with a strong acid. 5) A process according to claim 4 wherein the strong acid is hydrochloric, hydrobromic, sulfuric, fosforic, methanesulfonic, p-toluenesulfonic or camphorsulfonic acid. 6) A process according to claim 5 wherein the strong acid is methanesulfonic acid. 7) A process according to claim 4 wherein an alcoholic solvent is used. 8) A process according to claim 7 wherein the solvent is methanol. 9) A method for the preparation of Diltiazem, the method comprising conducting the process according to claim 1 and thereafter synthesizing the Diltiazem from the III-(2S,3S) enantiomer obtained in the process. A process which allows the re-use of compounds of formula in diltiazem synthesis through a process of conversion to a mixture of enamiomers III-(2R,3R) and III- (2S.3S) is described.

Full Text

The following Specification Particularly describes and ascertains the nature of this invention and the manner in which it is to be performed
The present invention relates to a method for the preparation of thero-2-hydroxy-3-(2-aminophenylthio-3-(4-methoxyphenyl) propionic acid esters, intermediates useful for the synthesis of diltiazem, starting from (2R,3R)-2-hydroxy-3-(2-aminophenylghio)-3-(4-methoxyphenyl) propionic acid or derivatives thereof. The invention also relates to preparation of Dilitazem from the above proponic acid ester prepared by the method of this inventions. The method of the present invention permits recycle of a wate product of a conventional Diltiazem Synthesis. Diltiazem, (+)-(2S/3S)-3-acetoxy-5-[2-(dimethylamino)-2-dihydro-2(-4-methoxy-phenyl)-1,5-benzothiazepin-4(5H)-one (The Merck Index, XII ed., no. 3247, page541) is a known drug with calcium-antagonist activity described in the U.K. patent 1236467 (Tanabe Seiyaku Co. Ltd.).
Several methods for the preparation of diltiazem such as, for example, those described in the above cited U.K. patent 1236467, in the European patent application 0 059 335 and in the Japanese patent no. 71/8982, all in the name of Tanabe Seiyaku Co. Ltd., are described in the literature.
Most of these methods substantially provide for the following synthetic scheme. Scheme 1
-1-
wherein R is a hydrogen atom or a lower alkyl and the asterisks mark the stereogenic carbon
atoms.
These methods use the compound of formula III-threo in racemic form, as intermediate. 8 However, diltiazem shows stereocenters with S configuration and then, following the above
reported scheme, a step for the separation of the (2S,3S) enantiomer from the (2R,3R) one is
necessary.
The separation of the two enantiomers can be carried out on the intermediate (III) in the
form of an ester (R=alkyl) as well as in the form of acid (R=H). 10 For example, the separation of the (2S,3S) and (2R,3R) enantiomers at the level of the ester
III can be carried out by using an optically active acid as resolving agent (U.S. patent
5,144,025 - Zambon Group S.p.A.) or by spontaneous resolution (U.S. patent 5,097,059 -
Zambon Group S.p.A.).
In case of separation at the level of the acid III, the methods described in the literature 15 provide for, inter alia, the resolution with optically active bases such as a-phenylethylamine
(European patent application 0 098 892 - Tanabe Seiyaku Co. Ltd) or L-lysine (U.K. patent
application 2130578 - Istituto Luso Farmaco d'ltalia S.p.A.) or the acylation in the presence
of a lipase (European patent application 0 617 130 - Orion-Yhtyma Oy Fermion).
It is evident that the resolution methods have the disadvantage of giving the desired (2S,3S) 20 isomer with a maximum theoretical yield of 50% on the raceme and of giving also the
corresponding (2R,3R) isomer, at the same time.
The isomers with (2R,3R) configuration, unsuitable for diltiazem synthesis, are then a waste
product in the industrial synthesis.
As a consequence, a process for the recycle of said compounds in order to recover them for 25 diltiazem synthesis should be useful.
As far as we know, the only method described in the literature for the recycle of an
intermediate with (2R,3R) configuration is the process claimed in the U.S. patent 5,102,999
(Zambon Group S.p.A.) which provides for the racemization of the intermediate IV-cis
(2R,3R).
-3-
We have now found a process for the conversion of the intermediates 1II-(2R,3R) to a mixture of enantiomers III-(2R,3R) and III-(2S,3S) which allows to re-use the enantiomers with (2R,3R) configuration of the intermediates of formula III for diltiazem synthesis. Therefore, object of the present invention is a process for the conversion of thero-(2R,3R)-2-hydroxy-3-(2-aminophenylthio)-3-(4-methoxypbenyl)propionic acid derivatives of formula
10
,OCH3
III-(2R,3R)
wherein R1 is a linear or branched C1-C3 alkyl or a hydrogen atom;
into a mixture of enantiomers III-(2R,3R) and III-(2S,3S),
characterised by the following steps:
(a) the cyclization of the compound III-(2R,3R) to afford the corresponding compound of
formula
20 I0CH3
1V-(2R,3R)
75
30
(b) the conversion to the compound of formula
wherein R2 is a hydrogen alkyl or a C3Cacyl group, (C) the reduction to afford the compound of formula
(d) the opening reaction by treating with a strong acid or with a strong base in an alcoholic or aqueous solvent.
The mixture of the two enantiomers III-(2R,3R) and III-(2S,3S) obtained with the process object of the present invention can be resolved according to the already reported methods in order to separate the (2S, 3S) enantiomer and if desired, preparing Diltiazem from the said enantiomer.
In the present context, if not otherwise specified, the term mixture of enantiomers means a substantially racemic mixture (2R,3R:2S,3S ratio about 1:1) or a mixture wherein the enantiomer 2S,3S prevails.
Analogously, if the absolute configuration of the compounds of formula III or IV is not indicated, it means that said compounds are a substantially racemic mixture (2R,3R:2S,3S ratio about 1:1) or a mixture wherein the enantiomer 2S, 3S prevails.
The term liner or branched C1-C3 alkyl means methyl, ethyl, propyl, isopropyl and the term C2-C4 acyl group means acetyl, propionyl, butyryl, isobutyryl.
The intermediates of formula III-(2R,3R) used as starting products in the process object of
the present invention are known compounds and they are obtained as waste products in the
optical separation processes for diltiazem synthesis. Generally the compounds of formula III are in the form of acid (R1=H) or in the form of
methyl (Rl=CH3) or ethyl (R1-CH2-CH3) ester.
Preferably, in the process object of the present invention the compounds of formula III are
methyl esters.
The cyclization of the compound III-(2R,3R) to afford the compound of formula IV-(2R,3R) can be carried out according to the known methods for the cyclization of the corresponding
(2S,3S) enantiomer. For example, the cyclization of the esters of formula I1I-(2R,3R) can be
carried out by treating with fosfonic acids (U.S. patent 5,223,612 - Zambon Group S.p.A.) or
by treating with sulfonic acids (European patent application 0 447 135 - Tanabe Seiyaku Co.
Ltd.). Similarly, the cyclization of the acid of formula III-(2R,3R) can be carried out by treating with sulfonic acids (European patent application 0 395 323 - Tanabe Seiyaku Co.
Ltd.) or with bases (European patent application 0 450 705 - Stamicarbon B.V.).
In the process object of the present invention the cyclization reaction is preferably carried
out starting from the methyl ester of formula II1-(2R,3R) by treating with cis-propenyl-
fosfonic acid. The subsequent conversion reaction to the derivative V can be carried out according to
known methods too. For example, the methods described in J. Org. Chem., 1996, 8586-8590
or in the already cited U.S. patent 5,102,999 can be used.
Preferably, in the process object of the present invention, the acetyl derivative of formula V
(R2=COCH3) is prepared, then hydrolysed to afford the compound Va which will be in equilibrium with its tautomer (Vb) as herein below reported.
30
,OCH3
.OCH,
Va Vb
The subsequent reduction reaction allows to obtain the compound IV-cis.
Optionally, the hydrolysis and reduction reactions can be carried out one-pot, that is in an
unique reaction environment, without isolating the compound Va (or Vb).
The preparation of the acetyl derivative V is preferably carried out by treating with acetic
anhydride in dimethylsulfoxide, in the presence of catalytic amounts of pyridine. The optional hydrolysis can be then carried out by treating with bases such as sodium hydroxide
or sodium mesylate.
The reduction of the compound of formula V can be carried out with known methods, for
example by treating with hydrides according to what reported in the already cited U.S.
patent 5,102,999. The resultant compound IV-cis can be then converted into the corresponding compound of
formula III-threo by treating with a strong acid or with a strong base in an alcoholic or
aqueous solvent.
The amount of acid or base is at least equimolar, preferably in excess, with respect to the
compound IV-cis. Generally the reaction is carried out by using an excess of acid or base equal to 10%-30% in
moles with respect to the compound IV.
The strong acids used in the process of the invention are inorganic acids such as
hydrochloric, hydrobromic, sulfuric and fosforic acid or organic acids such as sulfonic acids,
preferably methanesulfonic,p-toluenesulfonic and camphorsulfonic acid. Sodium hydroxide is preferably used as strong base.
-7-
In the process object of the present invention methanesulfonic acid is preferably used.
The reaction is carried out in an alcoholic solvent such as, for example, methanol or ethanol,
preferably in methanol, or in water optionally in admixture with a suitable co-solvent such as dimethylsulfoxide.
Dependently on the alcohol used as solvent the corresponding ester of formula III-threo is
obtained which can then be used again in the preparation of diltiazem according to the
synthetic route illustrated in the previous scheme 1.
When an aqueous solvent is used, the acid (R1=H) of formula III-threo is obtained and used according to the synthetic route illustrated in scheme 1 too.
The opening reaction of the compound of formula IV-cis represents the most characterising
feature of the process object of the present invention.
In fact, as far as we know, this reaction has never been described in the literature.
On the contrary, cyclizations of the compound III-threo to afford the compound IV-cis by using acids or bases have widely been described in the literature.
Furthermore, it is evident that the possibility of racemizing the waste products with (2R,3R)
configuration of the process for diltiazem preparation at the level of one of the earliest
synthetic intermediates represents a relevant advantage from a practical and economic
viewpoint. 20 A particularly preferred embodiment of the process object of the present invention is the
following.
The methyl ester of formula III-(2R,3R), obtained by resolution of the corresponding
racemic mixture, is cyclized with cis-propenyl-fosfonic acid and then oxidised by treatment
with acetic anhydride/dimethylsulfoxide/pyridine obtaining the acetyl derivative of formula V. After basic hydrolysis and reduction with sodium borohydride the racemic compound IV-
cis is obtained and treated with an excess of methanesulfonic acid in methanol up to the
obtainment of the racemic methyl ester III-threo.
The resolution of the racemic methyl ester III-threo allows to obtain the compound III-
(2S,3S) which is used for diltiazem synthesis and the enantiomer III-(2R,3R) which can undergo a further recycle phase according to the process object of the present invention.
¦8-
In order to better illustrate the present invention the following examples are now given.
Example 1
A mixture of methyl (2R,3R)-2-hydroxy-3-(2-aminophenylthio)-3-(4-metrioxyphenyl)- propionate (5 g; 15 mmoles) and cis-propenyl-fosfonic acid (0.183 g; 1.5 mmoles) in xylene
(35 ml) was heated under reflux and stirring for 5.5 hours.
After distilling a xylene/methanol mixture (about 3%), the reaction mixture was cooled to
15°C.
The resultant precipitate was filtered under vacuum, washed with xylene (2x5 ml) and then dried in oven at 65°C obtaining (2R,3R)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-l,5-
benzothiazepin-4(5H)-one (42 g; 89.6% yield).
Example 2
A catalytic amount of pyridine (19.7 g; 0.25 moles) was added to a solution of (2R,3R)-2,3-
dihydro-3-hydroxy-2-(4-methoxyphenyl)-l,5-benzotbiazepin-4(5H)-one (500 g; 1.66 moles) in dimethylsulfoxide (1100 g; 14.1 moles) and acetic anhydride (425 g; 4.17 moles). After
keeping the solution under stirring at room temperature for 24 hours, water (1000 ml) was
slowly added and the mixture was kept under stirring for 30 minutes.
The crystalline precipitate was filtered off, washed with methanol and dried obtaining 3-
acetoxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (481.6 g; 84.7% yield).
Example 3
A suspension of 3-acetoxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (17.1 g; 0.05
mmoles) in methanol (51 ml) was cooled in ice and then a solution of NaOH (5 g; 0.125
mmoles) in water (63 ml) was added.
The solution was then kept under stirring for 2 hours at room temperature. After neutralisation with HCL 2N (50 ml) and extraction with ethyl acetate, the organic
phase was washed twice with water, dried and concentrated obtaining a viscous oil.
The oil was treated with ethyl ether obtaining 2-(4-methoxyphenyl)-l,5-benzothiazepin-
3,4(2H,5H)-dione (12.98 g; 86.7% yield) as a crystalline solid.
Example 4
Sodium borohydride (0.567 g; 15 mmoles) was added to a solution of 2-(4-methoxyphenyl)-1,5-benzothiazepin-3,4(2H,5H)-dione (4.25 g; 14.2 mmoles) in methanol (65 ml), kept under stirring, at 15°C.
After 1 hour the reaction mixture was poured into a buffer solution (100 ml) at pH 7 and methanol was removed by distillation under vacuum. The resultant mixture was extracted with methylene chloride (2x30 ml). After evaporation of the solvent under reduced pressure cis-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (4.15 g; 97% yield) as racemic mixture was obtained.
Example 5
In a 500 ml flask, equipped with thermometer and condenser, cis-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one (50 g; 165.9 mmoles) was suspended in methanol (200 ml) and methanesulfonic acid (19.2 g; 200 mmoles) was added to the mixture.
The reaction mixture was brought to reflux and the progress of the reaction was followed by TLC (eluent ethylacetate:hexane=6:4). After heating for 7 hours, the content of starting product was about 1%.
The reaction mixture was then cooled at room temperature before adding, with a dropping funnel, a 8% solution of sodium bicarbonate (208 g; final pH=7.0).
The resultant precipitate was filtered off and washed three times with water (3x50 ml). The
resultant solid was then dried at 50°C under vacuum up to constant weight obtaining methyl
threo-2-hydroxy-3-(2-aminophenylthio)-3-(4-inethoxyphenyl)-propionate (54 g; molar yield
94.2%; HPLC titre 96.5%).
Example 6
30% Sodium methylate in methanol (0.1 ml; 0.5 mmoles) and, after 15 minutes, sodium borohydride (54 mg; 1.42 mmoles) were added to a suspension of 3-acetoxy-2-(4-methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (1 g; 2.9 mmoles) in methanol (5 ml). After keeping the mixture under stirring at room temperature for 5 hours, methanesulfonic acid (0.6 ml; 9.3 mmoles) was added and the mixture was heated under reflux for 5 hours.
-
After cooling at room temperature, 8% sodium bicarbonate (9 ml) was added.
After adding toluene, the resultant solid was filtered off, washed with water (3x1 ml) and
dried at 50°C under vacuum obtaining methyl threo-2-hydroxy-3-(2-aminophenylthio)-3-(4- methoxyphenyl)propionate (0.92 g; molar yield 90%; HPLC titre 95%).
Example 7
30% Sodium methylate in methanol (0.5 ml; 2.5 mmoles) and, after 15 minutes, sodium
borohydride (0.28 g; 7.35 mmoles) were added to a suspension of 3-acetoxy-2-(4-
methoxyphenyl)-l,5-benzothiazepin-4(5H)-one (5 g; 14.7 mmoles) in methanol (25 ml). After keeping the mixture under stirring at room temperature for 5 hours, a 6.5M solution of
hydrochloric acid in methanol (2.7 ml; 17.6 mmoles) was added and the mixture was heated
under reflux for 5 hours.
After cooling at room temperature, 8% sodium bicarbonate (16 ml) was added.
The resultant precipitate was filtered off, washed with water (3x5 ml) and dried at 50°C under vacuum obtaining methyl threo-2-hydroxy-3-(2-aminophenylthio)-3-(4-
methoxyphenyl)-propionate (5 g; molar yield 85%; 1H-NMR titre 75%).
We claim,
1. A process for the conversion of threo-(2R,3R)-2-hydroxy-3-(2-aminophenyltIiio)-3-(4-methoxyphenyl)propionic acid derivatives of formula
uridtiiOH COOR1
.OCH,
III - (2R,3R)
wherein R is a linear or branched C1-C3 alkyl or a hydrogen atom; into a mixture of enantiomers III-(2R,3R) and III-(2S,3S), characterised by the following steps:
(a) the cyclization of the compound III-(2R,3R) to afford the corresponding compound of formula
IV-(2R,3R)
wherein R2 is a hydrogen atom or a C2-C4 acyl group,
( c) the reduction of the compound of formula (V) of step (b) to afford the compound of formula
OH
OCH3
IV - cis
(d) the opening reaction by treating with a strong acid or with a strong base in an alcoholic or aqueous solvent to get the mixture of enantiomers of formula III-(2R, 3R)and III(2S,3S)
2) A process according to claim 1 for the conversion of the methyl ester of threo (2R,3R)- 2-hydroxy -3-(2-aminophenylthio)-3-(4-methoxyphenyl)-propionic acid.
3) A process according to claim 1 wherein the compound of formula V wherein R is
acetyl is used.
4) A process according to claim I wherein the opening reaction is carried out by
treating with a strong acid.
5) A process according to claim 4 wherein the strong acid is hydrochloric,
hydrobromic, sulfuric, fosforic, methanesulfonic, p-toluenesulfonic or
camphorsulfonic acid.
6) A process according to claim 5 wherein the strong acid is methanesulfonic
acid.
7) A process according to claim 4 wherein an alcoholic solvent is used.
8) A process according to claim 7 wherein the solvent is methanol.
9) A method for the preparation of Diltiazem, the method comprising conducting the process according to claim 1 and thereafter synthesizing the Diltiazem from the III-(2S,3S) enantiomer obtained in the process.
A process which allows the re-use of compounds of formula
in diltiazem synthesis through a process of conversion to a mixture of enamiomers III-(2R,3R) and III- (2S.3S) is described.

Documents:

01873-cal-1998 abstract.pdf

01873-cal-1998 claims.pdf

01873-cal-1998 correspondence.pdf

01873-cal-1998 description(complete).pdf

01873-cal-1998 form-1.pdf

01873-cal-1998 form-18.pdf

01873-cal-1998 form-2.pdf

01873-cal-1998 form-3.pdf

01873-cal-1998 form-5.pdf

01873-cal-1998 letters patent.pdf

01873-cal-1998 p.a.pdf

01873-cal-1998 reply f.e.r.pdf

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Patent Number

208190

Indian Patent Application Number

1873/CAL/1998

PG Journal Number

29/2007

Publication Date

20-Jul-2007

Grant Date

19-Jul-2007

Date of Filing

22-Oct-1998

Name of Patentee

ZAMBON GROUP S.P.A.

Applicant Address

An Italian Company, Via della Chimica 9, 36100 Vicense, Italy.

Inventors:

#	Inventor's Name	Inventor's Address
1	PIETRO ALLEGRINI	VIA S. CHIARA 27, 36045-LONIGO-(VI), ITALY.
2	GAETANO MARCHIORO	VIA CALVI 80,36100-VICENA,
3	GIUSEPPE BARRECA	CORSO A.DE GASPERI 22,37042-CALDIERD- (VR)
4	MARCO VILLA	VIALE LUNIGIANA 10,20125 MILLANO
5	LAURA RUSSO	VIA VSILLA 156/C,20153 MILANO,

PCT International Classification Number

C07C 323/02, C07C319

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	M197A002374	1997-10-22	Italy