Title of Invention

PROCESS FOR PREPARATION OF PENAM DERIVATIVES FROM CEPHAM DERIVATIVES

Abstract

The present invention provides a novel process for preparing a 2(3-heterocyclyl methyl penam derivatives of the formula (I): which comprises : (iv) reacting a compound of formula (VII) where L represents a leaving group with a compound of formula (VIII) where Het is as defined earlier in the presence of a solvent and base at a temperature in the range of -10 to 110 °C to produce a compound of formula (IX) (v) oxidizing the compound of formula (IX) using conventional oxidizing agents in the presence of water miscible solvent and an organic acid to produce a compound a formula (I) and if necessary (vi) de-esterifying the compound of formula (I) where R1 represents a carboxy protecting group to a compound of formula (I) where R, represents hydrogen using metal catalyst, in the presence of a base and water immiscible solvent.

Full Text

Field of the Invention The present invention relates to a process for preparing 2a-methyl-2p-substituted methyl penam derivatives from cepham derivatives. More particularly the present invention provides a novel process for preparing a 2p-heterocyclyl methyl penam derivatives of the formula (I) wherein Ri represents hydrogen, carboxylic acid protecting group such as an ester or a pharmaceutically acceptable salt; R2 and R3 may be same or different and independently represent hydrogen, halogen, NH2, acylamino, phthalimido with a proviso that both R2 and R3 are not NH2, acylamino, phthalimido; Het represents a 5 or 6 membered NH containing heterocycle ring system containing one or more heteroatoms selected from O, S, or N. The 2P-heterocyclyl methyl penam derivatives of the formula (I) is used as p-lactam antibiotic. The utility of p-lactam antibiotics is limited by the resistance exhibited by the microorganisms, through the action of p-lactamase enzyme. The enzyme acts through cleavage of p-lactam ring of these antibiotics, thereby destroying the drug leading to loss of activity. It therefore requires P-lactamase inhibitors, which can counteract with the P-lactamase enzyme and eliminate the drug resistance. The P-lactamase inhibitors are used along with P-lactam antibiotics to promote the antibiotic activity. Thus research on new penam derivatives and novel processes for their production is continuing. Background of the Invention Several patents have disclosed various methods of producing 2(i-substituted methyl penam derivative. For instance, US patents 4529592, 4562073, & 4668514 and EP 97446 discloses a process, which involves treatment of 2P-azidomethyl penam derivatives of the formula (II): wherein R is a carboxy-protecting group, with acetylene/acetylene derivative or vinyl derivative under high pressure in a sealed reactor and at elevated temperatures followed by deprotection with a suitable reagent to get the (3-lactamase inhibitor of the formula (I). The 2p-azidomethyl penam derivative of the formula (II) was in turn prepared from the 2p-substituted methyl penam derivatives of the formula (III) wherein R = carboxy-protecting group; X = chloro or bromo, by treating with sodium azide in aqueous polar aprotic solvents, followed by oxidation. The above method suffers from the limitation of introducing only very few heterocycles like 1,2,3-triazole group, but not a wide variety of other heterocycles. In addition, the method requires handling of acetylene gas at high pressure and high temperature, which carries inherent hazard owing to its high detonation velocity, thus rendering it non industrial and eco-friendly. Added to it, this process also requires handling of excess sodium azide, leaving behind large quantities of azide for ETP treatment which is hazardous owing to the release of hydrazoic acid as it is a potential explosive and a serious health hazard. The EP 0273699 disclosed a different approach, which involves the preparation of 2p-triazolylmethylpenam derivatives of the formula (IV) wherein X = chlorine or bromine; R is carboxy-protecting group, with 1H-1,2,3-triazole. The product obtained can be oxidized and deprotected to get the 2p-substituted methyl penam derivatives of the formula (I). The EP 306924 disclosed a reduction method employing lead compounds like lead chloride or lead bromide to prepare 2P-triazolylmethyl penam derivative of the formula (IV) (n=0-2) from 6,6-dibromo-2p-triazolylmethyl penam derivative of the formula (V). where R represents carboxy-protecting group, is treated with 2-trimethylsilyl-1,2,3-triazole in a sealed tube at elevated temperatures to give a mixture, which requires purification by column chromatography to isolate the 2p-triazolylmethyl penam derivative of the formula (IV) (n=0). In most of the methods involved, 2p-halomethylpenam of the formula (HI) is used as the key intermediate. This is true with both the azide route and the triazole route discussed above. However, the five-membered 2-halomethyl penam of the formula (III) itself is an unstable intermediate and therefore manufacturing of this intermediate in large quantities and storing are always cumbersome to handle. This intermediate has been found to degrade on storage even at low temperatures in isolated form as well in the solvent from which it is isolated. Thus all the operations related to preparation of this intermediate have to be done rapidly and the isolated intermediate has to be converted to the final product immediately. As a result of these limitations, the scale up in plant always affords less yield and low quality, which ultimately leads to low level of consistency. All the above described processes are associated with one or more of the following limitations: (i) unstable nature of the key intermediate (ii) use of hazardous and explosive reagents (iii) requirement of high pressures coupled with elevated temperatures - especially with acetylene (iv) use of large excess of sodium azide and its consequent environmental and explosion issues (v) use of highly toxic and polluting compounds of heavy metals like lead, especially in the penultimate stages of pharmaceuticals. These factors affect the consistency in quality and yield of the intermediates and the final product as well as safety on manufacturing scale. To overcome the foregoing limitations, we were searching for a novel process, which involves stable intermediates and safe reagents/reaction conditions to manufacture 2p~substituted methyl penams. In our laboratory, we conducted extensive research and investigated a variety of synthetic schemes and methodologies to find a novel solution for manufacturing the said penam. Objective of Invention The main objective of the present invention is to provide a process for the preparation of 2P-heterocyclyl methyl penam derivatives of the formula (I), which involves the conversion of six-membered cepham moiety. Another objective of the present invention is to provide a process for the preparation of 2p-heterocyclyl methyl penam derivatives of the formula (I), in good yields and high purity. Still another objective of the present invention is to provide a process for the preparation of 2p-heterocyclyl methyl penam derivatives of the formula (I), in pure form and not contaminated with the other isomers. As a result of our continued efforts, we could identify a new route, which employs a cepham moiety unlike the penam derivatives employed so far. The advantage of the application of the six-membered cepham moiety is that it is a stable intermediate unlike the penams employed so far, and therefore utilization of this intermediate would reflect in overcoming the limitations discussed above. While in all the available literature 2p-chloromethylpenams of the formula (III) were employed to prepare 2(3-triazolylmethyl substituted penams of the formula (IV), whereas the present invention relies on ring-contraction phenomenon of converting the six-membered 3-halomethyl cephams of the formula (VII) in to 2p-heterocyclyl methyl penams of the formula (I). Summary of the Invention Accordingly, the present invention provides a process for the preparation of 2p-heterocyclyl methyl penam derivatives of the formula (I), wherein Ri represents hydrogen, carboxylic acid protecting group such as an ester or a pharmaceutically acceptable salt; R2 and R3 may be same or different and independently represent hydrogen, halogen, NH2, acylamino, phthalimido with a 1 proviso that both R2 and R3 are not NH2, acylamino, phthalimido; Het represents a 5 or 6 membered NH containing heterocycle ring system containing one or more heteroatoms selected from O, S, orN, which comprises: (i) reacting a compound of formula (VII) where L represents a leaving group with a compound of formula (VIII) where Het is as defined earlier in the presence of a solvent and base at a temperature in the range of-10 to 110 °C to produce a compound of formula (IX) (ii) oxidizing the compound of formula (IX) using conventional oxidizing agents in the presence of water miscible solvent and an organic acid to produce a compound a formula (I) and if necessary (iii) de-esterifying the compound of formula (I) where Ri represents a carboxy protecting group to a compound of formula (I) where Ri represents hydrogen, using metal catalyst in the presence of a base and water immiscible solvent. The process is as shown in Scheme-1 Detail Description of the Invention: In an embodiment of the present invention carboxy-protecting group such as ester is selected from p-nitrobenzyl, p-methoxyphenyl, diphenylmethyl, and the like. In another embodiment of the present invention L represents a leaving group selected from halogen like chloro, bromo, iodo; p-toluenesulphonyloxy, methanesulphonyloxy. In yet another embodiment of the present invention the group represented by Het is selected from pyrrolyl, pynrolidinyl, piperidinyl, imidazolyl, oxazolidinyl, 1,2,3-triazolyl, 1,2,4-triazolyl etc. In still another embodiment of the present invention the group represented by acylamino is selected from phenacetylamino, phenoxyacetylamino or benzoylamino. In still another embodiment of the present invention, the reaction between the 3-substituted cepham derivative of the formula (VII) and with compound of formula (VIII) is carried out in a suitable solvent in the presence or absence of a phase transfer catalyst in the presence or absence of a base. The molar ratio of the compound of formula (VIII) is about 1 to 30 times, preferably about 1 to 10 times with respect to the cepham compound of the formula (VII). The heterocyclic amine_used can either be in free form or as its salt of a mineral acid or an organic sulphonic or carboxylic acid. The solvents do not play a major role and therefore a wide variety of solvents such as ethereal solvents like THF, dioxane, diglyme, monoglyme, etc, polar aprotic solvents like DMF, DMAC, DMSO, acetone, ethyl acetate, sulpholane, acetonitrile, etc, protic solvents like n-butanol, isopropanol, methanol, ethanol, cyclohexanol, etc, aromatic solvents like toluene, anisole, etc., chlorinated solvents like dichloroethane, dichloromethane, carbon tetrachloride, chlorobenzene, etc, can be used. These organic solvents can be used as a single solvent or a combination or witn some amount of water as an additional component. In the case of water-immiscible solvents, the reaction is conducted in biphasic medium using a phase transfer catalyst under vigorous agitating conditions. The phase transfer catalyst can be a quaternary ammonium salt like tetrabutylammonium bromide, benzyltributylammonium bromide, benzyltrioctylammonium bromide, etc., or a phosphonium salt like benzyltriphenylphosphonium bromide, etc. The base can be inorganic or organic, and preferably an inorganic oxide or a carbonate of alkali or alkaline earth metal like magnesium carbonate, calcium carbonate, cesium carbonate, barium carbonate, potassium hydrogen carbonate, sodium carbonate, sodium hydrogen carbonate, copper oxide, copper carbonate, potassium carbonate, etc. The temperature of the reaction is normally between -10 to 110 °C, and preferably between 30 to 65 °C. The product obtained from the above reaction can be either purified to remove the unwanted isomers or taken directly to next step without purification, as the product obtained in the next step takes care of removing impurities and isomers, thereby affording pure compound. The product thus obtained is isolated in paste form and oxidized with an oxidizing agent in aqueous acidic medium. The oxidizing reagent is a conventional sulfur-oxidizer like potassium permanganate, peracetic acid, trifluoroperacetic acid, m-chloroperbenzoic acid, oxone, etc, preferably potassium permanganate. The oxidation can be conducted in the presence of an organic acid like aliphatic carboxylic acid, aliphatic sulphonic acid, etc., preferably acetic acid, methane sulphonic acid, etc. The reaction temperature can vary from -30 to +50 °C, and preferably from -10 to +30 °C. The time required for the reaction can very from 15 min to 8 hours, preferably 15 min to 2 hours. At the end of the reaction, the reaction mixture is quenched with a suitable reagent to destroy the excess oxidizing reagent and the reaction medium is neutralized with an inorganic base like sodium bicarbonate. At this stage, the product undergoes a purification process in ethyl acetate wherein other isomers of the reaction are getting solubilized in this solvent. The selectivity of purification to remove unwanted isomers of the process is less in other solvents and ethyl acetate is a preferred solvent for getting pure-required-isomer. The 2p-triazolylmethyl substituted penam of the formula (IX) thus obtained was converted to 2p-triazolylmethylpenam derivative of the formula (I) by a suitable de-esterification methodology depending on the type of carboxyl-protecting group. For instance, in the case of the p-nitrobenzyl protecting group, the following methodology illustrates the deprotection to obtain the P-lactam inhibitor of the formula (I). The 2p~triazolylmethyl substituted penam of the formula (I) (n=2; R is a carboxy-protecting group) is converted to the compound of the formula (I) (n=2; R=H) in the presence of a noble metal catalyst, in the presence of an inorganic base in a biphasic medium in the presence of a hydrogen source at elevated pressures. The noble metal catalyst can be 5-10% Pd/C, 5% Pt, Adam's catalyst, etc., and preferably 10% Pd. The reaction is conducted in the presence or absence of an organic or inorganic base, and preferably in the presence of an inorganic base. The inorganic base is a carbonate of alkali or alkaline earth metal and preferably sodium bicarbonate. While the reaction can be conducted in a monophasic or biphasic medium, preferably an aqueous-organic biphasic medium is used, comprising of water-immiscible solvent such as toluene, ethyl acetate, methyl acetate, etc., and preferably ethyl acetate. After work up, the product was isolated by crystallization from the aqueous medium. The process of producing the 2P-triazolylmethylpenam derivative of the formula (I) is described in detail in the reference examples given below which are provided by way of illustration only and should not be considered to limit the scope of the invention. It is interesting to note that in the five-membered penam derivative obtained from the six-membered cepham derivative, the stereochemical course of the reaction pathway is favorable to produce the p-isomer selectively. In addition, during the ring contraction of the 3-substituted cepham derivatives of the formula (VII) into 20-heterocyclylmethyl penam derivatives of the formula (I), the configuration of the carboxyl group is unchanged. The carboxyl group is trans to the 2p-triazolylmethyl group. The relative stereochemistry has been confirmed by NOE experiments unequivocally. Examples: Example 1: Preparation of 4-Nitrobenzyl 2P-(lH-l,2,3-triazol-l-ylmethyl)-2a-methyIpenam- 3 To a solution of 4-nitrobenzyl 3-bromo-3-methylcepham-4-carboxylate (50 gm) in acetone (250 mL) contained in a 2 Lit RB flask was added water (65 mL) and 1H-1,2,3-triazole (100 mL) at room temperature. To the clear solution, calcium carbonate (25 gm) was added under vigorous stirring. The reaction mixture was heated to 50-60 °C over a period of 15 min and maintained under vigorous stirring at this temperature for period of 9 hrs. The progress of the reaction was monitored by TLC. After the reaction was over, the reaction mixture was filtered to remove the inorganic salts and the bed washed with acetone (50 mL). The clear solution was distilled under vacuum to remove acetone at less than 30 °C. The solution after removal of acetone was poured in to dichloromethane (250 mL) and stirred well at 26-28 °C. The organic layer was separated and washed with purified water (200 mL) four times. The organic layer was concentrated under vacuum to remove dichloromethane, initially at Example 2: Preparation of 4-Nitrobenzyl 2p-(lH-l,2,3-triazoH-ylmethyl)-2a-methylpenam- 3a-carboxylate-l,l-dioxide of the formula (I): To acetic acid (350 mL) at 20 °C in a 2 Lit RB flask, was added 4-nitrobenzyl 2p-(lH-l,2,3-triazol-l-ylmethyl)-2a-methylpenam-3a-carboxylate (as obtained from the above example) and purified water (35 mL). The homogeneous reaction mixture was cooled to 5-10 °C under stirring. To the homogeneous reaction mixture, powdered potassium permanganate (30 gm) was added in 12 lots over a period of 1.5 -2.0 hrs while maintaining the temperature at 5-10 °C. Stirring was continued for another 0.5 hrs and the reaction was monitored by TLC. After the reaction was over, the reaction mixture was charged into crushed ice (500 gm) under vigorous stirring over a period of 0.5-1.0 hrs. To the mass, cold ethyl acetate (500 mL) was added while maintaining the temperature at 0-5 °C. A dilute solution of hydrogen peroxide (25%; 40 mL) was added slowly over a period of 1 hr at such a rate that the temperature was maintained at 0-5 °C. After the decolourization was complete, ethyl acetate (200 mL) was added. To the solution, which was almost colorless, sodium chloride (100 gm) was added and stirred well for 15 min. The ethyl acetate layer was separated and washed with water (250 ml) twice. To the ethyl acetate layer, 8% sodium bicarbonate solution (-400 mL) was added slowly until pH of the aqueous layer was >7.2. The reaction mixture was stirred for another 15 min and the pH checked again. After the pH stabilized at >7.2, stirring was stopped and the layers separated. The organic layer was washed with water (250 mL) twice and charcoalized with activated carbon (10 gm). The organic layer was concentrated to remove ethyl acetate under vacuum up to 150 mL when the product separated out from the medium. After maintaining under stirring for 5 hrs, the material was filtered and washed with ethyl acetate (30 mL). Drying under vacuum afforded colorless 4-nitrobenzyl 2p-(lH-l,2,3-triazoM-ylmethyl)-2a-methylpenam-3a-carboxylate 1,1-dioxide in pure form in 50-75% yield. Mass m/e: M+l peak at 436.3; !H NMR data (CDC13): 5 1.29 (3H, s, 2a-Me), 3.53 (1H, dd, J = 1.9 & 16.3 Hz, 7H-trans), 3.61 (1H, dd, J = 4.3 & 16.3 Hz, 7H-cis), 4.63 (1H, s, CH-CO2), 4.66 (1H, dd, J = 1.9 & 4.2 Hz, 6H), 5.07 (2H, Abq, J = 15.1 Hz, 2p-CH2), 5.35 (2H, Abq, J = 14 Hz, COO-CH2), 7.61 (2H, d, J = 8.7 Hz, aromatic ortho protons), 8.30 (2H, d, J = 8.7 Hz, aromatic meta protons), and 7.75 & 7.79 (2H, triazole protons). Example 3: Preparation of 2p-(lH-l,2,3-triazol-l-ylmethyl)-2a-methylpenam-3a-carboxyIic acid -1,1-dioxide of the formula (I): In to a 2 Lit high-pressure hydrogenator, ethyl acetate (500 mL), 10% Pd/C (2.5 gm), and 4-nitrobenzyl 2p-(lH-l,2,3-triazol-l-ylmethyl)-2a-methylpenam-3a-carboxylate 1,1-dioxide (25 gm) were added. The heterogeneous reaction mixture was cooled to 20-22 °C under stirring. A solution of sodium bicarbonate (24 gm in 375 mL of purified water) was added over 10-15 min at 20-22 °C. The hydrogenator was flushed with nitrogen and hydrogen pressure of 200 psi was applied over 10 min at 20-22 °C. The hydrogen pressure was maintained for 1.5-2.0 hrs and the progress of the reaction monitored. After the reaction was over, the hydrogen pressure was released and flushed with nitrogen. The reaction mass was cooled to 0-5 °C. The catalyst Pd/C was recovered by filtration and the bed washed with chilled purified water (50 mL). The aqueous layer was separated and washed with ethyl acetate (150 mL) three times. The pH was set to 6.4-6.6 with 6N HC1 (-37 mL required) and the aqueous layer washed with ethyl acetate (150 mL). The aqueous layer was charcoalized with activated carbon (4 gm) over 15 min and the bed washed with purified water (50 mL). The pH was set to 3.2 with 6N HC1 (-60 mL) and maintained for 15 min. Crystallization occurred. Stirring was continued at this pH for 30 min. The pH was further set to 2.5-2.6 with 6N HC1 (-15 mL) and maintained for 2 hrs. The crystals were filtered and washed with water followed by ethyl acetate (40 mL). The material was dried under vacuum for 5 hrs at 26-30 °C. The yield of the product, 2P-(1H-1,2,3-triazol-1 -ylmethyl)-2a-methylpenam-3a-carboxylic acid-1,1 -dioxide, was around 85-90%. Mass m/e: M-l peak at 299.1; lH NMR data (DMSO-d6): 5 1.33 (3H, s, 2a-Me), 3.31 (1H, dd, J = 1.4 & 16.5 Hz, 7H-trans), 3.71 (1H, dd, J = 4.5 & 16.5 Hz, 7H-cis), 4.80 (1H, s, CH-C02), 4.91 (1H, d, J - 15.3 Hz, H' of 2(3-CH2), 5.19 (1H, dd, J = 1.5 & 4.4 Hz, H6), 5.24 (1H, d, J = 15.3 Hz, H" of 2p-CH2), and 7.8 & 8.1 (2H, triazole protons). The stereochemistry of the 2ct-methyl and 2p-methylene groups was confirmed by NOE experiments. We claim : 1. A process for the preparation of 2p-heterocyclyl methyl penam derivatives of the formula (I), wherein R4 represents hydrogen, carboxylic acid protecting group such as an ester or a pharmaceutically acceptable salt; R2 and R3 may be same or different and independently represent hydrogen, halogen, -NH2? acylamino, phthalimido with a proviso that both R2 and R3 are not -NH2, acylamino, phthalimido; Het represents a 5 or 6-membered -NH-containing heterocycle ring system containing one or more heteroatoms selected from O, S, or N, which comprises : (i) reacting a compound of formula (VII) wherein L represents a leaving group and all other substituents are as defined above with a compound of formula (VIII) wherein Het. is as defined above, in the presence of a solvent and base at a temperature in the range of -10 to 110 °C to produce a compound of formula (IX)- where all substituents are as defined above, (ii) oxidizing the compound of formula (IX) using conventional oxidizing agents in the presence of water miscible solvent and an organic acid to produce a compound a formula (I) and (iii) de- esterifying the compound of formula (I) wherein R\ represents a carboxy protecting group to a compound of formula (I) wherein Ri represents hydrogen, by using metal catalyst in the presence of a base and water immiscible solvent. 2. The process according to claim 1, wherein the solvent used in step (i) is selected from THF, dioxane, diglyme, monoglyme, DMF, DMAC, DMSO, acetone, ethyl acetate, sulpholane, acetonitrile, n-butanol, isopropanol, methanol, ethanol, cyclohexanol, toluene, anisole, dichloroethane, dichloromethane, carbon tetrachloride, chlorobenzene or mixtures thereof. The process according to claim 1, wherein the base used in step (i) is selected from magnesium carbonate, calcium carbonate, cesium carbonate, barium carbonate, potassium hydrogen carbonate, sodium carbonate, sodium hydrogen carbonate, copper oxide, copper carbonate or potassium carbonate. The process according to claim 1, wherein the oxidizing agent used in step (ii) is selected from potassium permanganate, peracetic acid, trifluoroperacetic acid, m-chloroperbenzoic acid or oxone. The process according to claim 1, wherein the organic acid used in step (ii) is selected from acetic acid or methane sulphonic acid. The process according to claim 1, wherein the de-esterification in step (hi) is carried out using 5-10% Pd/C, 5% Pt or Adam's catalyst. The process according to claim 1, wherein the base used in step (iii) is selected from magnesium carbonate, calcium carbonate, cesium carbonate, barium carbonate, potassium hydrogen carbonate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate or sodium bicarbonate. The process according to claim 1, wherein the water-immiscible solvent used in step (iii) is selected from toluene, ethyl acetate or methyl acetate. The process according to claim 1, wherein the carboxy protecting group is selected from p-nitrobenzyl, p-methoxyphenyl or diphenylmethyl. The process according to claim 1, wherein the leaving group L is selected from chloro, bromo, iodo, p-toluenesulphonyloxy or methanesulphonyloxy. The process according to claim 1, wherein the group represented by Het is selected from pyrrolyl, pyrrolidinyl, piperidinyl, imidazolyl, oxazolidinyl, 1,2,3-triazolylor 1,2,4-triazolyl. The process according to claim 1 wherein the acylamino group is selected from phenacetylamino, phenoxyacetylamino or benzoylamino. The process according to claim 1, wherein the compound of formula (I) formed is a p isomer.

Documents:

434-MAS-2002 FORM-3 27-06-2011.pdf

434-mas-2002-abstract.pdf

434-mas-2002-assignment.pdf

434-mas-2002-claims duplicate.pdf

434-mas-2002-claims original.pdf

434-mas-2002-correspondence others.pdf

434-mas-2002-correspondence po.pdf

434-mas-2002-description complete duplicate.pdf

434-mas-2002-description complete original.pdf

434-mas-2002-form 1.pdf

434-mas-2002-form 3.pdf

434-mas-2002-form 5.pdf

434-mas-2002-other documents.pdf

434-mas-2002-pct.pdf