Title of Invention	A PROCESS OF PREPARING A NEEM OIL AND TURPENTINE OIL BASED WOUND HEALING OINTMENT OR CREAM
Abstract	A neem and turpentine oils based wound healing ointment or cream comprising 10 - 20% by weight of neem and turpentine oils in the volume ratio 1:4 to 4:1, 1.5 - 3.0 % by weight of zinc oxide, 0.5 -2.0% by weight of salicylic acid, 0.5 - 2.0 % by weight of potassium nitrate,5 - 15% by weight of linseed oil and 50 to 80% by weight of additive base.

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FORM 2 THE PATENTS ACT, 1970 As amended by the Patents (Amendment) Act, 2002 COMPLETE SPECIFICATION (See Section 10; Rule 13) TITLE A process of preparing a neem oil and turpentine oil based wound healing ointment or cream APPLICANTS Godrej Agrovet Limited, Pirojshanagar, Eastern Express Highway, Vikhroli (East), Mumbai 400 079, Maharashtra, India, an Indian Company INVENTORS Under Section 28(2) Iyer Ramaswamy, Dr Vyas Brahmanand Ambashankar, Dr Mistry Keki Bamanshaw and Godrej Nadir Burjor, all of Godrej Agrovet Limited, Pirojshanagar, Eastern Express Highway, Vikhroli (East), Mumbai 400 079, Maharashtra, India, all Indian Nationals The following specification particularly describes the nature of this invention and the manner in which it is to be performed FIELD OF INVENTION This invention relates to a process of preparing a neem oil and turpentine oil based wound healing ointment or cream. PRIOR ART Antibacterial and antifungal properties of neem oil and turpentine oil are known. It is, however, difficult and cumbersome to apply and retain neem oil and turpentine oil on wounds for healing purpose due to the free flowing nature of the oils. Therefore, use of neem oil and turpentine oil for wound healing application is inconvenient, cumbersome and time consuming. Turpentine oil is also known to act as maggot repellent. Regeneration of tissues is an integral part of healing. Activities such as cell proliferation, smoothening of the skin or restoration of the epithelial cells also facilitate healing. Linseed oil, zinc oxide and salicylic acid are reported to possess these activities. Antiseptic properties of salicylic acid and zinc oxide are also reported. [The Merck Index - 12th Edn 1996, Publ: Merck & Co. Inc. NJ USA] OBJECTS OF INVENTION An object of the invention is to provide a neem oil and turpentine oil based wound healing ointment or cream which has improved wound healing properties. Another object of the invention is to provide a neem oil and turpentine oils based wound healing ointment or cream which is easy and convenient to apply on wounds. Another object of the invention is to provide a neem oil and turpentine oil based wound healing ointment or cream which is economical. Another object of the invention is to provide a neem oil and turpentine oil based wound healing ointment or cream which is without side effects and is safe. Another object of the invention is to provide a process of preparing a neem oil and turpentine oil based wound healing ointment or cream which has improved wound healing properties. Another object of the invention is to provide a process of preparing a neem oil and turpentine oil based wound healing ointment or cream which is easy and convenient to apply on wounds. Another object of the invention is to provide a process of preparing a neem oil and turpentine oil based wound healing ointment or cream which is economical. Another object of the invention is to provide a process of preparing a neem oil and turpentine oil based wound healing ointment or cream which is without side effects and is safe. Another object of the invention is to provide a process of preparing a neem oil and turpentine oil based wound healing ointment or cream which is simple, easy and convenient to carry out. Another object of the invention is to provide a method of treatment of wounds by applying topically the above neem oil and turpentine oil based wound healing ointment or cream. DESCRIPTION OF THE INVENTION According to the invention there is provided a neem oil and turpentine oil based wound healing ointment or cream comprising 10 - 20% by weight of neem oil and turpentine oil in the volume ratio 1:4 to 4:1, 1.5 to 3.0 % by weight of zinc oxide, 0.5 to 2.0% by weight of salicylic acid, 0.5 to 2.0% by weight of potassium nitrate, 5 to 15% by weight of linseed oil and 50 to 80% by weight of additive base. Preferably the additive base comprises bees wax and petroleum jelly in the weight ratio 15 : 40 to 25 : 70. Preferably the wound healing ointment or cream comprises 16.5% by weight of neem oil and turpentine oil in the volume ratio 3:1, 1.65% by weight of zinc oxide, 0.8% by weight of salicylic acid, 0.8% by weight of potassium nitrate, 10.0% by weight of linseed oil and 70.25% by weight of additive base, namely bees wax and petroleum jelly in the weight ratio 23 : 77. According to the invention there is also provided a process of preparing a neem oil and turpentine oil based wound healing ointment or cream comprising the steps of (i) melting 50 to 80% by weight of an additive base at 40 to 100°C; (ii) mixing 10 to 20% by weight of neem oil and turpentine oil in the volume ratio 1:4 to 4:1 and 5 to 15% by weight of linseed oil with the additive base melt under stirring followed by cooling the mixture to 20 to 50 ° C; (iii) and further mixing the mixture obtained by step (ii) with 1.5 to 3.0% by weight of zinc oxide, 0.5 to 2.0% by weight of salicylic acid and 0.5 to 2.0% by weight of potassium nitrate under stirring. Preferably the process comprises melting the additive base namely bees wax and petroleum jelly in the weight ratio 15 : 40 to 25 : 70. Preferably the process comprises the steps of ia) melting the additive base, namely bees wax and petroleum jelly in the weight ratio 23 : 77; iia) mixing 16.5% by weight of neem oil and turpentine oil in the volume ratio 3:1 and 10.0% by weight of linseed oil with the additive base melt followed by cooling; iiia) and further mixing the mixture obtained by step (iia) with 1.65% by weight of zinc oxide, 0.8% by weight of salicylic acid and 0.8% by weight of potassium nitrate. Preferably the additive base is melted at 75 ° C. Preferably the mixture of step (ii) or (iia) is cooled to 40 ° C. Besides bees wax and petroleum jelly other additive base such as paraffin wax, salts and esters of fatty acids, fatty alcohols or hydrogenated wax may also be used in the ointment or cream of the invention. According to the invention there is also provided a method of treatment of wounds by applying topically the neem oil and turpentine oil based wound healing ointment or cream described above. The medicament of the invention may be used on any mammalian species. The following experimental example is illustrative of the invention but not limitative of the scope thereof. Example 1 Bees wax (16.5 % by weight) and petroleum jelly (53.75% by weight) were melted at 75 °C. Neem oil and turpentine oil (16.5% by weight in the volume ratio 3:1) and linseed oil (10.0% by weight) were mixed with the melt under stirring for half an hour. After cooling the mixture to 40°C , zinc oxide (1.65% by weight), salicylic acid (0.8% by weight) and potassium nitrate ( 0.8% by weight) were further mixed with the above mixture under stirring for one hour to form an ointment. The wound healing efficacy of the ointment was studied on fresh wounds in dogs and cattle and the results were as given in the following Tables: Table 1 Data on wound healing in dogs Medicament Time taken to start wound healing in days (Mean ± S.E) Time taken for complete wound healing in days (Mean ±SE) Neem oil and turpentine oil in the volume ratio 3:1 4.41 ±0.51 21.34 ±2.64 Ointment of Example 1 2.83 ±0.33 16.50 ±2.38 Acrilin ointment 2.33 ±0.33 15.33 ±1.05 Table 2 Data on wound healing in cattle Medicament Time taken to start wound healing in days (Mean ± S.E) Time taken for complete wound healing in days (Mean ±SE) Neem oil and turpentine oil in the volume ratio 3:1 5.12 ± 0.48 36.2 ±2.80 Ointment of Example 1 2.67 ± 0.33 29.5 ± 2.29 Acrilin ointment 3.0 ±0.26 30.33 ±2.65 The frequency of application of the medicaments on the wounds was twice daily namely morning and evening. Acrilin is a commercially available ointment of GSK Agrivet, India comprising gamma benzene hexachloride, proflavin hemisulphate and cetrimide. It is quite evident from Tables 1 and 2 that the ointment of the invention showed improved wound healing properties as compared to neem oil and turpentine oil alone and that the wound healing properties of the ointment of the invention were comparable to those of the commercially available ointment Acrilin. The ointment of the invention is easy and convenient to apply on wounds. It is economical because neem oil and turpentine oil and the other ingredients are cheap and easily available. The ointment of the invention is also safe and without side effects as the ingredients are without side effects. Besides being in the form of ointment, the medicament of the invention also may be in the form of a cream. The process of preparing the ointment or cream of the invention is simple, easy and convenient to carry out. WE CLAIM : 1) A neem and turpentine oils based wound healing ointment or cream comprising 10 - 20% by weight of neem and turpentine oils in the volume ratio 1:4 to 4:1, 1.5 - 3.0 % by weight of zinc oxide, 0.5 -2.0% by weight of salicylic acid, 0.5 - 2.0 % by weight of potassium nitrate,5 - 15% by weight of linseed oil and 50 to 80% by weight of additive base. 2) A neem and turpentine oils based wound healing ointment or cream as claimed in claim 1, wherein the additive base comprises bees wax and petroleum jelly in the weight ratio 15: 40 to 25 : 70. 3) A neem and turpentine oils based wound healing ointment or cream as claimed in claim 1, comprising 16.5% by weight of neem and turpentine oils in the volume ratio 3:1, 1.65% by weight of zinc oxide, 0.8%) by weight of salicylic acid,0.8% by weight of potassium nitrate, 10.0% by weight of linseed oil and 70.25% by weight of additive base namely bees wax and petroleum jelly in the weight ratio 23 : 77. 4) A process of preparing a neem and turpentine oils based wound healing ointment or cream comprising the steps of (i) melting 50 to 80% by weight of an additive base at 40 to 100°C; (ii) mixing 10 - 20% by weight of neem and turpentine oils in the volume ratio 1:4 to 4:1 and 5 -15% by weight of linseed oil with the additive base melt under stirring followed by cooling the mixture to 20-50°C; (iii) and further mixing the mixture obtained by step (ii) with 1.5 - 3.0 % by weight of zinc oxide, 0.5 - 2.0% by weight of salicylic acid and 0.5 -2.0% by weight of potassium nitrate under stirring. 5) A process as claimed in claim 4, which comprises melting the additive base namely bees wax and petroleum jelly in the weight ratio 15 : 40 to 25: 70. 6) A process as claimed in claim 4, which comprises the steps of ia) melting the additive base, namely bees wax and petroleum jelly in the weight ratio 23 : 77; iia) mixing 16.5% by weight of neem and turpentine oils in the volume ratio 3:1 and 10.0% by weight of linseed oil with the additive base melt followed by cooling; iiia) and further mixing the mixture obtained by step (iia) with 1.65% by weight of zinc oxide, 0.8% by weight of salicylic acid and 0.8% by weight of potassium nitrate. 7) A process as claimed in anyone of claims 4-6, wherein the additive base is melted at 75 ° C. 8) A process as claimed in any one of claims 4 to 7, wherein the mixture of step (ii) or (iia) is cooled to 40 ° C. 9) A process of preparing a neem and turpentine oils based wound healing ointment or cream as herein described particularly with reference to Example 1. Dated this 26th day of April 2004 (Jose M A) of Khaitan & Co Agent for the Applicants

Documents:

486-mum-2004-abstract(10-3-2005).pdf

486-mum-2004-cancelled pages(11-11-2005).pdf

486-mum-2004-claims(amanded)-(11-11-2005).pdf

486-mum-2004-claims(complete)-(27-4-2004).pdf

486-mum-2004-claims(granted)-(29-6-2007).pdf

486-mum-2004-correspondence(20-04-2006).pdf

486-mum-2004-correspondence(ipo)-(1-8-2007).pdf

486-mum-2004-correspondence(ipo)-(29-06-2007).pdf

486-mum-2004-description(complete)-(27-4-2004).pdf

486-mum-2004-description(granted)-(29-6-2007).pdf

486-mum-2004-form 1(27-04-2004).pdf

486-mum-2004-form 19(07-06-2004).pdf

486-mum-2004-form 2(complete)-(27-4-2004).pdf

486-mum-2004-form 2(granted)-(29-6-2007).pdf

486-mum-2004-form 2(title page)-(complete)-(27-4-2004).pdf

486-mum-2004-form 2(title page)-(granted)-(29-6-2007).pdf

486-mum-2004-form 26(27-04-2004).pdf

486-mum-2004-form 3(27-04-2004).pdf

486-mum-2004-form 8(07-06-2004).pdf

486-mum-2004-specification(amanded)-(10-3-2005).pdf