Title of Invention

A NOVEL PROCESS FOR THE MANUFACTURE AND PURIFICATION OF COMPACTIN

Abstract

An improved method for the manufacture and purification of Compactin comprising - loading a contained bioreactor with solid state nutritive matrix and sterilizing it, - mixing the said sterilized solid state nutritive matrix with Penicillium citrinum, - incubating the said inoculated solid state nutritive matrix for 4 -6 days at 25 –30degereeC, - extracting the fermented matrix with an organic solvent or an aqueous buffer~ - concentrating the extract obtained, and re-extracting in ethyl acetate~ optionally giving the ethyl acetate extract a wash with saturated NaCI solution - concentrating the ethyl acetate extract and cooling the slurry obtained at 0 to 10 °C for 4 to 12 hours; - filtering to get crude crystals, optionally giving a hexane wash;. -dissolving the crude crystals in a water miscible solvent followed by the addition of filter aid and agitating for 0.5 to 3 hour;. - filtering get a clear solution which is concentrated and -crystallizing of the concentrate to get pure compactin.

Full Text

Documents:

in-pct-2002-1600-che abstract.pdf

in-pct-2002-1600-che claims granted.pdf

in-pct-2002-1600-che correspondence others.pdf

in-pct-2002-1600-che correspondence po.pdf

in-pct-2002-1600-che description (complete) granted.pdf

in-pct-2002-1600-che form 1.pdf

in-pct-2002-1600-che form 13.pdf

in-pct-2002-1600-che form 19.pdf

in-pct-2002-1600-che form 3.pdf

in-pct-2002-1600-che form 5.pdf

in-pct-2002-1600-che pct.pdf

in-pct-2002-1600-che power of attorney.pdf