Title of Invention

A PROCESS OF PREPARING ESSNTIAL PHOSPOFIDYICHOLINE 73-79%

Abstract This invention relates to a process of preparing EPL-7O Powder (As Phospotidylcholine 73 - 79%) from soya Lecithin liquid. More particularly this invention relate to a process of preparing EPL-7O powder, from Soya lecithin, which is more effective and very much useful for food and drug preparation, specifically considering low cholesterol level, necessary for lever treatment.
Full Text
FORM 2
THE PATENTS ACT, 1870 (39 of 1970)
COMPLETE SPECTFICATION [See section 10]
A process of preparing essential phospholipid powder containing phospotidylcholine 73 ~ 79%
Shah Amit Navnit
401/601, Ashwamegh Complex Near Mithakhail Under Bridge, Navrangpura, Ahemedabad. Gujarat
(India).
The following specification particularly describes the nature of the invention and the manner in which it is to be performed.

Indian National


This Invention relates to a process of preparing essential phospholipid powder, particularly to a process of preparing essential phospholipid powder containing phospotfdytcholine 73 - 79% and more particularly to a process of preparing essential phospholipid powder containing phospottdylchoRne 73 -79% from soya Lecithin liquid, which is more effective and very much useful for food and drug preparation, specifically considering low cholesterol level, necessary for lever treatment.
Presently essential phospholipid powder containing phospolidylcholine 73 -79% is obtained by extraction process from soybeans, which is more effective end much useful for drug preparation. The main object of this inventor is to provide a process of preparing essential phospholipid powder containing phospotfdytcholine 73 - 79%, from Soya tecitlin liquid, which is more effective and very much useful for food and drug preparation specifically considering tow cholesterol level necessary for lever treatment.
Accordingly this invention provides a process of preparing essential phospholipid powder containing phospolidylcholine 73 - 79% comprising the following steps:
i) extracting soya lecithin Liquid using Ethyl/lsopropyl Alcohol in an extractor, precipitating out EPL containing phosphatidylcholine 73-79 % as a top layer and other phospholipids at bottom and transferring the top layer/miscella, into a storage vessel.
ii) distillatiing the said miscella by passing through a heat exchanger for condensing the Alcohol vapours for reuse, and recovering EPL liquid through a flasher.
iii) filtering the said EPL liquid containing phospotfdytcholine and storing in a vessel,
iv) extracting EPL liquid using Acetone/Ether/IPA in an extractor by settling out EPL liquid containing phosphatidylcholine as precipitate at the bottom.
v) drying the said precipitate in slurry form, preferably In a double cone
vacuum dryer, to remove Acelone/Elther/lPA vapours and forming
lumps/cake,
vf) further drying the said lumps/cake in a fluid bed dryer for removing any




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odour and moisture,
vfl) miffing the dry tumps, preferably in a multimlll and pulverizing In a
pulverizer forming EPL powder containing phosphotidylcholine.
viii) passing the said powder through a shifter for obtaining uniform size
particles , ix) optionally passing the said powder through ethylene oxide in an Inert atmosphere of nitrogen, to make it free form any bacteria, before packing in bags or drums and then sealing the same.


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The process of preparing essential phospholipid powder containing phospotidylchollne 73 - 79% from Soya Lecithin liquid will be clear from the following description, by way of the flow diagram in the accompanying drawings and the following example.
Fig No. 1.shows the flow diagram of the extraction of the essential
phospholipid containing phospotidylchollne In liquid form and recovery of
alcohol.
Fig. No 2 shows the flow diagram of the extraction of the essential
phospholipid containing phospotfdytcholine in powder form and recovery of
ETHER/1PA/ACETONE.
Fig. No. 3.showsthe chemical formula of phospotidylchollne.
EXAMPL E:
Take charge of Ethyl Alcohol, 800 Kgs. In an extractor under stirring and feeding slowly Soya lecithin liquid 400 Kgs. Continue stirring about six hours and put up for self ling for one hours, precipitating our EPL containing phospholidylchollne as a top layer and other phospholipids settled as a bottom layer which is collected into a drum, top layer miscefla is transferred into storage vessel and than to a distillation una, the heat exchanger of which is heated to a temperature of 75° C at vacuum of 760 mm/Hg, the miscella is passed through the heal exchanger. The Ethyl Alcohol vapours condense through condenser and collected into a receiver for reuse. EPL liquid Is passed through a flasher and then filtered and the titration is stored En vessel in a dry place at temperature of 35 to 40° C. EPL containing phosphatidylcholine is in oil form now. As an alternative Iso propyl alcohol can be used in place of ethyl alcohol.


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Now take charge of distilled Acetone, 2000 Kgs. In an extractor, add EPL liquid, 200 Kgs. slowly, wider continuous stirring for an hour, continuing stirring for about four hours, maintaining temperature of 25 lo 30e C at atmospheric pressure during extraction and then settling for half an hour. After settling, separating out EPL containing p ho sphotldy I choline precipitate from bottom layer and Acetone and fatty oil mixture, called miscella, at top layer through middle valve of the extractor, and passing through the distillation unit comprising heat exchanger for heating the mis eel la and a Hasher for separating fatty acid oil and condenser for condensing Acetone vapors and receiving them in a Receiver and storing in a storage tank for reuse. The EPL containing phospotfdytcholine preefpffate maybe added wftn binder such as phosphorous in a ratio of about 10 to 20% wAv. of EPL liquid.
The EPL containing phosp hot Idyl choline precipitate is dried In double cone vacuum dryer at temperature of 60 to 80° C and pressure of 760 mm/Hg. for three hours and then cooling to 30° C to remove Acetone vapour and forming EPL lump/cake. Further drying the EPL lump on a fluid bed drier for 50 minutes for removing any odors and moisture, milling the said EPL lump in a muttimill and than pulverizing the same in a pulverizer at room temperature of about 20° C, forming EPL powder of 60 to 80 mesh size, passing the EPL powder through a shifter to obtain EPL powder with uniform particles size preferably of 60 mesh.
The EPL powder is now passed through an atmosphere of Ethylene oxide and Nitrogen and packed in sealed drums/bags.
Ethylene oxide is passed for 15 to 20 minutes in an innert atmosphere of Nitrogen through which EPL powder Is passed for making ft free from any bacteria before packing.
Ether or IPA can also be used in place of Acetone for extraction.


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The process of preparing EPL from Soya lecithin is given in the flow chart accompanying this specification.
The stage I of the flow chart shows the process steps of preparing essential phospholipid containing phospotldylchollne 73 - 79% In liquid form from Soya lecithin liquid and the stage II of the flow chart shows the process steps of preparing essential phospholipid containing phospotidylcholtne 73 - 79% in powder form from the liquid of stage I.
The chemical structure of essential phospholipid containing phospotidylcholtne 73 - 79% Is shown in figure 3.
The present invention yield In 55 to 65% , Soya acid oil - 30 to 40% and moisture 1 to 2%.
The above description with reference to example and the flow chart is given to understand the invention rather than 1o Hmtt lis scope.


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essential phospholipid powder containing phospotldvlchollne 73 -
79 %
SPECIFICATION
Physical State & Appearance Form. White to cream powder
A very hygroscopic. Odourless
Odour Taste Testless or bland taste Soluble in
Solubility benzene, hexane, partially soluble
in alcohol, forms stable emulsion
in water.
Acetone Insoluble Comosttion: Min 98 %
P - Choline Min 73 %
P - Eta no amine Min 15 %
P - Inositols Phosphalidtc Acid Min 7%
Moisture 12 %
Acid Value Max l%
Total ash Max 26
Total Phosphorus as P Max 15 %
Total Nitrogen as N M ax 3 %
Peroxide Value Max 1.2 %
Max l
Hexane Insoluble 0.50 %
pH Value 6.0-7.0
Shelf Life Two years
Packing In HDPE bag in fiber
drums of 10 Kgs.






I CLAIM:
1. A process of preparing essential phospholipid powder containing
phospolldylchollne 73 - 79% comprising the following steps:
i) extracting soya lecithin Liquid using etnyl/isopropyl alcohol in an extractor, precipitatiing out essential phospholipid containing phosphotldyfcholine 73-79 % as a top layer and other phospholipids at bottom and transferring the top layer/miseella into a storage vessel,
ii) dist illating the said miscella by passing through a heat exchanger for condensing the alcohol vapours for reuse, and recovering EPL liquid through a flasher,
III) filtering the said EPL liquid containing phos photidy(choline and storing in a vessel,
iv) extracting EPL liquid using Acetone/Ether/I PA in an extractor by settling out EPL liquid containing phos phot idylc ho line as precipitate at the bottom,
v) drying the said precipitate in slurry form, preferably En a double cone
vacuum dryer, to remove Acetone/Efther/IPA vapours and forming
lumps/cake,
vf) further drying the said lumps/cake in a fluid bed dryer for removing any
odour and moisture,
vii) milling the dry tumps, preferably in a multimili and pulverizing in a
pulverizer forming EPL powder containing phosphotldylchollne,
viii) passing the said powder through a shifter for obtaining uniform size
particles, ix) optionally passing the said powder through ethylene oxide in an inert atmosphere of nitrogen, to make it free form any bacteria, before packing In bags or drums and then sealing the same.
2. A process as claimed in claim 1, wherein the said essential phospholipid


powder containing phospotidylcholine 73 - 79% powder is made to pass through a shifter of 60 to 80 mesh size, preferably 60 mesh size for obtaining uniform size particles.
3. A process as claimed In claim 1 or 2, wherein the said essential phospholipid powder containing pho spot idyl choline 73 - 79% powder is passed through ethylene oxide, in an inert atmosphere of nitrogen, for making it free form any bacteria and packing into sealed drums/begs.
4. A process as claimed in claim 1, wherein the said Soya lecithin liquid and the said ethyl alcohot/isopropyl alcohol is taken In a ratio of 1 :2 w/w. and the said soya lecithin liquid is added slowly into extractor under continuous stirring for about six hours and then settled for about an hour.

5. A process as claimed in claim 1, wherein the essential phospholipid powder containing phospotidylcholine 73 - 79% in Squid form and the acetone/ ether/jpa is taken in ratio of 1:10 w/w and the liquid is added slowly for about an hours under continuous stirring for about four hours maintaining temperature of 25 to 30° C at atmospheric pressure and then settled for about half an hour.
6. A process as claimed in claim 1, wherein the precipitate of essential phospholipid powder containing phospotidylcholine 73 - 79% is dried in double cone vacuum dryer at the temperature of 60 to 80" C and pressure of 760 mm/Hg for about three hours and then coofed to temperature of 30' C.
7. A process as claimed in claim 1, wherein the acetone used is distilled acetone and recycled after extraction and distillation.
8. A process as cfaimed in claim 1, wherein the precipitate slurry of step (iv), in claim 1, is added with binder such as phosphorous in a ratio of 10 to 20% w/w. of the EPL mass.
9. A process as claimed 1, wherein the milling and pulverteing of lumps of essential phospholipid powder containing phospotidylcholine 73 - 79% Is carried out at room temperature of about 20" C.
10.A process of preparing essential phospholipid powder containing phospotidylcholine 73 - 79% from soya lecithin liquid, substantially as herein described and illustrated in the example and flow diagram.
Dated this 25th July 2002.


Documents:

1123-mum-2002-abstract(24-1-2003).pdf

1123-mum-2002-abstract(granted)-(9-9-2005).pdf

1123-mum-2002-cancelled pages(12-1-2005).pdf

1123-mum-2002-claims(16-12-2002).pdf

1123-mum-2002-claims(granted)-(9-9-2005).pdf

1123-mum-2002-correspondence(12-1-2005).pdf

1123-MUM-2002-CORRESPONDENCE(IPO)-(22-1-2004).pdf

1123-mum-2002-description(complete)-(16-12-2002).pdf

1123-mum-2002-description(granted)-(9-9-2005).pdf

1123-mum-2002-drawing(12-1-2005).pdf

1123-mum-2002-drawing(16-12-2002).pdf

1123-mum-2002-drawing(24-1-2003).pdf

1123-mum-2002-drawing(granted)-(9-9-2005).pdf

1123-mum-2002-form 1(12-1-2005).pdf

1123-mum-2002-form 1(16-12-2002).pdf

1123-MUM-2002-FORM 2(COMPLETE)-(16-12-2002).pdf

1123-MUM-2002-FORM 2(GRANTED)-(9-9-2005).pdf

1123-mum-2002-form 2(title page)-(complete)-(16-12-2002).pdf

1123-mum-2002-form 2(title page)-(granted)-(9-9-2005).pdf

1123-mum-2002-form 3(16-12-2002).pdf

1123-mum-2002-specification(amended)-(12-1-2005).pdf


Patent Number 196016
Indian Patent Application Number 1123/MUM/2002
PG Journal Number 29/2010
Publication Date 16-Jul-2010
Grant Date
Date of Filing 16-Dec-2002
Name of Patentee SHAH AMIT NAVNIT
Applicant Address 401/501, ASHWAMEGH COMPLEX, NEAR MITHAKHALI UNDER BRIDGE, NAVRANGPURA AHMEDABAD-380009
Inventors:
# Inventor's Name Inventor's Address
1 SHAH AMIT NAVNIT 401/501, ASHWAMEGH COMPLEX, NEAR MITHAKHALI UNDER BRIDGE, NAVRANGPURA AHMEDABAD-380009
PCT International Classification Number A61K031/56
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA