Title of Invention	METHOD OF 2D-NMR CORRELATION SPECTROSCOPY WITH DOUBLE QUANTUM FILTRATION FOLLOWED BY EVOLUTION OF SINGLE QUANTUM TRANSITIONS
Abstract	The invention relates to a method of 2-dimensional nuclear magnetic resonance (2D-NMR) correlation spectroscopy, comprising a pulse sequence with the following steps: i) excitation of double quantum coherence; ii) immediate reconversion to single transition single quantum coherence; iii) evolution of the set of single transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. The method can achieve an improved sensitivity, has a transition selectivity and suppresses diagonal peaks.

Title of Invention

METHOD OF 2D-NMR CORRELATION SPECTROSCOPY WITH DOUBLE QUANTUM FILTRATION FOLLOWED BY EVOLUTION OF SINGLE QUANTUM TRANSITIONS

Abstract

The invention relates to a method of 2-dimensional nuclear magnetic resonance (2D-NMR) correlation spectroscopy, comprising a pulse sequence with the following steps: i) excitation of double quantum coherence; ii) immediate reconversion to single transition single quantum coherence; iii) evolution of the set of single transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. The method can achieve an improved sensitivity, has a transition selectivity and suppresses diagonal peaks.

Full Text	2 Such a method is known, e.g., from A. Bax et al., J. Am. Chem. Soc. 1980, 102, p. 4849-4851 (compare Ref. 2). Nuclear magnetic resonance (NMR) is a powerful tool for investigating the chemistry of a sample. NMR measures the reaction of the spins of the nuclei within the sample upon excitation with high frequency electromagnetic pulses. 2D NMR1 techniques are especially useful to identify spin correlations which in turn reflect the bonding or spatial topology of the molecule. For low abundance spins, the correlation information has been produced by exciting Double Quantum Coherence (DQC)2,3 which is subsequently reconverted following evolution by either a single pulse (of flip angle typically 45°, 60°, 90°, 120° or 135°), or alternatively by a cluster of pulses as in the 2D INADEQUATE CR sequence4"6. 2D INADEQUATE CR has been designed to provide the maximum sensitivity and results in transition selectivity in F2. These experiments result in spectra whose F1 frequencies are sums of the chemical shifts of each pair of coupled spins. COSY- or SECSY-type displays for 13C spin systems are also known in the literature7'8. These methods afford sub-optimal sensitivity, however, there being no transition selectivity. Object of the invention It is the object of the invention to propose a method of 2D-NMR correlation spectroscopy which can achieve an improved sensitivity and which has a transition selectivity. Short description of the invention This object is achieved, in accordance with the invention, by a method of 2-dimensional nuclear magnetic resonance (2D-NMR) correlation spectroscopy, comprising a pulse sequence with the following steps: Bruker BioSpin AG 20.06.2006 P8968IN 3 i) excitation of double quantum coherence; ii) immediate reconversion to single transition single quantum coherence; iii) evolution of the set of single transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. This method is further referred to as LASSY. The object is also achieved, in accordance with the invention, by a method of 2D-NMR correlation spectroscopy, comprising a pulse sequence with the following steps: i) excitation of double quantum coherence; ii) immediate reconversion with a 90° pulse; iii) evolution of the set of anti-phase single quantum transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. This method is further referred to as LAPSY. The basic idea of the invention, as realized in LASSY and LAPSY, is to adopt a double quantum coherence excitation and an immediate reconversion; followed by a single quantum evolution. This is in contrast to the procedures'of the state of the art, where a double quantum evolution is followed by double quantum reconversion. The inventive approach employs a double quantum filter to discriminate against uncoupled spins, which is particularly useful for nuclei of low abundance in a sample. The subsequent conversion to single quantum coherence (SQC) introduces transition selectivity. The evolution of single quantum coherence further improves the spectral resolution and reduces relaxation losses, as compared to an evolution of DQC. In addition, with the inventive methods, diagonal peaks are suppressed. Bruker BioSpin AG 20.06.2006 P8968IN 4 Preferred variants of the inventive methods In a preferred variant of the inventive LASSY method, during step ii), a conversion to a single transition at each of the chemical shifts of two coupled spins is done. By this way, the transition selectivity can be achieved in a simple way. The selection of the desired component (high-field or low-field component of each doublet, or the so-called b or a component) may be achieved by employing suitable phases of the reconversion pulses. In another preferred variant of the inventive LASSY method, in step ii), the double quantum re-conversion is detuned with a first pulse having a flip angle other than 45°, preferably a reduced flip angle, in particular a flip angle of 20°-30°. This facilitates the measurement of shifts and couplings. In the following, variants of both above inventive methods (LASSY and LAPSY) are introduced. One preferred variant of the inventive methods provides that in step iv), isotropic mixing is done. This is a simple and fast way of doing the mixing step. In particular, a GD-1 sequence can be applied. In a further development of this variant, in step iv), isotropic mixing is performed with sequences optimized for chemical shift bandwidth and / or coupling bandwidth. As an advantageous alternative to the above mentioned variant, in step iv), a cascade of two bilinear rotations is applied. Although taking longer, this variant avoids bandwidth vs. power deposition issues. Bruker BioSpin AG 20.06.2006 P8968IN 5 Optionally, this variant may be further developed by the cascade of two bilinear rotations including pulse contraction. Another preferred variant of the inventive method is characterized in that a p pulse is applied just before or right after step iv). This permits echo pathway selection, while transferring coherence from one doublet component in F1 to the other doublet component in F2. Another preferred variant of the inventive methods provides that suitable offset compensated pulses or offset compensated groups of pulses are employed. Also preferred is a variant, wherein the pulse sequence is adapted for indirect detection. Then the improvement in sensitivity is particularly evident. In a highly preferred variant, the method is used with C-13 nuclei. C-13 can be used with the invention in natural abundance, as already practiced, avoiding any enriching of isotopes in the sample. In a practically important variant of the inventive methods, the method is used with solution samples. Alternatively, and also relevant in practice, the method is used with solid state samples, preferably applying magic angle spinning (MAS), in particular with Si-29 and/or C-13 nuclei. In a highly preferred variant of the inventive methods, the method is used with rare spin-1/2 systems. This variant can be applied with samples in any phase. The rarity of the spin-1/2 system guarantees single coupling between two spins-1/2. Note that according to the invention, a spin system is considered as rare if its abundance in the sample is 10% or less, and preferably 5% or less. In an alternative, also preferred variant, the method is used with spin-1-systems in ordered phases, in particular with H-2, Li-6, N-14. The ordered phase Bruker BioSpin AG 20.06.2006 P8968IN 6 guarantees quadrupolar coupling of a spin-1. This variant can be performed independent of the abundance of the spin-1 system. An ordered phase, suitable for the invention, is for example a liquid crystal. Finally, in a preferred variant, the method is adapted to generate magnitude mode spectra or pure phase spectra. Magnitude mode spectra can be produced by selecting the echo or anti-echo, employing phase cycling as described below or by using gradients. Pure phase spectra are also typically produced by using gradients or phase cycling in alternative procedures. Further advantages can be extracted from the description and the enclosed drawing. The features mentioned above and below can be used in accordance with the invention either individually or collectively in any combination. The embodiments mentioned are not to be understood as exhaustive enumeration but rather have exemplary character for the description of the invention. Detailed description of the invention and drawing The invention is shown in the drawing. Fig. 1 shows schematically a 2D LASSY sequence with isotropic mixing, in accordance with the invention; Fig. 2 shows schematically a 2D LASSY sequence with ZQ (zero quantum) planar mixing, in accordance with the invention; Fig. 3 shows an experimentally obtained 2D LASSY spectrum of 0.5M sucrose; Fig. 4 shows a 2D LAPSY sequence with isotropic mixing, in accordance with the invention. Bruker BioSpin AG 20.06.2006 P8968IN 7 Overview The invention proposes a new family of pulse experiments for correlation spectroscopy of low abundance spins, that results in diagonal suppression and optimal sensitivity with single transition 2D 'multiplet' structure in the cross peaks. The sequence also has better relaxation tolerance than corresponding DQ experiments. Implementation involves a double quantum filter followed by reconversion to single transition single quantum coherence, then evolution followed by isotropic mixing or a laboratory frame bilinear analog. These sequences are useful not only in 13C NMR in solution state, but also 29Si MAS NMR in the solid state and the NMR of spin-1 systems in ordered phases. An 'anti-phase' version of the experiment is also pointed out. More specifically, this document proposes - and demonstrates for 13C at natural abundance - a family of new pulse sequences to achieve the maximum sensitivity as predicted by the relevant unitary bound9, while also achieving a diagonal suppressed COSY display with transition selectivity. F1 spectral widths are now governed by the normal single quantum spectral window, resulting in superior resolution; furthermore, since it is Single Quantum Coherence (SQC) that evolves during t1 and not DQC, relaxation losses may be expected to be typically lower per unit time, as also the single quantum linewidths. The inventive approach The inventive approach is based on employing a double quantum filter to discriminate against uncoupled spins, followed by conversion to single quantum coherence with transition selectivity (i.e. conversion to a single transition at each of the chemical shifts of the two coupled spins). Evolution of the set of single transitions then follows, terminated by a zero quantum mixing Hamiltonian, followed by signal detection. Echo (or anti-echo) selection is governed by the addition (or not) of a p pulse just before / right after mixing. Bruker BioSpin AG 20.06.2006 P8968IN 8 Addition of the said pulse results not only in echo pathway selection, but also the selection of different multiplet components in F1 and in F2 (e.g. the low field component in F1 and the high field component in F2). As described below, this permits the spectroscopist to deduce shifts and couplings despite the selection of only one component out of the cross-peak multiplet - which should normally have four components. We call these experiments Low Abundance Single transition correlation SpectroscopY (LASSY) and dub them CLASSY for C-13 applications. The pulse sequences Figure 1 illustrates a 2D LASSY sequence with isotropic mixing. Here t=(2J)-1 D =10µS. Pulse flip angles are proportional to the width of the corresponding bars: the widest represent 180° pulses, while the narrowest represents a 45° (or shorter) pulse, the intermediate width representing 90° pulses. The phase cycling is given as: j1: {4(x),4(y),4(-x),4(-y)}; j2: {4(x),4(y),4(-x),4(-y),4(-x),4(-y),4(x),4(y)}; j3: {4(x),4(y),4(-x),4(-y)}; j4: {x, -y,-x, y}; j5: {8(x, -y,-x, y), 8(-x, y, x, -y)}; j6: {-y, -x, y, x}; j7: {128( x), 128(-x)}; jR: {-x, y, x, -y, x, -y, -x, y}. Figure 2 illustrates a 2D LASSY sequence with ZQ planar mixing. Here, t=(2J)-1, D=10 µs. The phase cycling is given by: j1,: {4(x),4(y)}; j2: {4(x),4(y),4(-x),4(-y)}; j3: {4(x),4(y)}; j4: {x, -y,-x,y}; j5: {4(x, -y,-x, y), 4(-x, y, x, -y)}; j6: {-y, -x, y, x}; j7: {32 (y), 32(-x), 32 (-y), 32(x)}; j8: {32 ( x), 32(y), 32 (-x), 32(-y)}; jR: {4(-X, y, x, -y, x, -y, -x, y), 4 (x, -y, -x, y, -x, y, x, -y)}. Mixing is accomplished by a suitable zero quantum Hamiltonian, that may be generated by isotropic mixing or by a cascade of two appropriate bilinear Bruker BioSpin AG 20.06.2006 P8968IN 9 rotations (which may include a pulse contraction)10. The sequence of transformations of the spin density matrix may be given as follows: Considering the further evolution of the transverse magnetization of the first spin, we find: In general, isotropic mixing of two spins / and S for an arbitrary duration t gives rise to: The implementation used here includes a terminal p pulse in the case of isotropic mixing (which we currently realize with the GD-1 sequence11); this permits echo pathway selection, while transferring coherence from one doublet component in F1 to the other doublet component in F2. In the implementation with a sequence of two bilinear rotations, this may be achieved by suitably setting the phase of the last pulse relative to that of the first pulse. It may be noted that mixing with a zero quantum effective Hamiltonian effectively Bruker BioSpin AG 20.06.2006 P8968IN 10 suppresses diagonal peaks, employing an appropriate mixing period x that may be chosen in light of the range of values of J in the molecule. The ratio of cross-peak to diagonal peak intensities can be shown in fact to be given by: It may further be noted that with either of the implementations indicated above, the shifts and couplings may be retrieved from the spectrum despite transition selectivity, although in practice the accuracy is a function of the F1 resolution. Analysis The scalar coupling and chemical shift may be calculated as follows. The LASSY spectrum of a coupled two-spin-1/2 system has two cross peaks, each with one multiplet component. These components correspond to different coupled spin states in the two dimensions. The (F1, F2) co-ordinates of the cross-peak detected at the second spin frequency are (v1+J/2, V2-J/2), v's being chemical shift frequencies expressed in Hz. The (F1, F2) co-ordinates of the cross-peak detected at the first spin frequency on the other hand are (V2+J/2, v1-J/2). The scalar coupling may be obtained by subtracting the F2 frequency of this cross peak from the F1 frequency of the other cross peak: The chemical shifts may now be easily obtained. Figure 3 illustrates a 2D LASSY spectrum of 0.5M sucrose. Isotropic mixing has been implemented employing the GD-1 sequence. 64 t1 points are acquired, each with 256 scans and 5k points in t2. Bandwidth vs. power deposition issues of isotropic mixing sequences may be avoided by employing two successive bilinear mixing sequences, although this does involve an extra mixing time of (2J)-1. Bruker BioSpin AG 20.06.2006 P8968IN 11 It is noted that 'detuning' the double quantum re-conversion sequence with the first pulse having a flip angle other than 45° (say, 20°-30°) also affords near-optimum sensitivity, while permitting the measurement of shifts and couplings, since it results in cross peaks that have one strong component and one weak component, diagonally displaced with respect to each other, with the intensity ratio of-3.73: 1 (for 30° flip angle of the first re-conversion pulse). These experiments avoid spectral registration by evolution of double quantum coherence. This has a significant consequence on resolution in F1 as well as on overall experiment sensitivity, since homonuclear double quantum coherence of rare spins in solution state is typically principally relaxed by their heteronuclear dipolar interactions with protons. Such double quantum relaxation is considerably more efficient than the relaxation of the corresponding single quantum coherence of rare spins, under extreme narrowing conditions. For larger molecular weights and/or higher magnetic field intensities, this is even more so, since the spectral density at zero frequency plays a significant role. Avoiding the evolution of DQC for ca. 100-200 ms and substituting this with DQ filtering (which lets the state of DQC persist for merely ca. 10-15 ms) could thus prove to be a major boon in biomolecular NMR as well. The LAPSY variant It is further noted that an alternative approach that involves excitation of DQC, followed by immediate reconversion with a 90° pulse, then spin evolution and mixing as before, also leads to diagonal suppressed correlation spectra. These spectra exhibit cross peaks, each with two multiplet components that are diagonally displaced. While this permits accurate quantification of the coupling constant, sensitivity is clearly sub-optimal (70% of the sensitivity of LASSY). Bruker BioSpin AG 20.06.2006 P8968IN 12 This experiment may be termed Low abundance Anti-Phase correlation SpectroscopY (LAPSY). The 2D LAPSY pulse sequence is shown in Figure 4. Figure 4 illustrates a 2D LAPSY sequence with Isotropic mixing. Here, x=(2J)-1, D=10 µs. Further variants Offset compensated versions of these experiments may be developed. The protocol may also be adapted for indirect detection. In particular, this set of experiments may also be extended to the solid state (e.g. 29Si MAS NMR) and to spin-1 systems (2H, 6Li, 14N) in ordered phases. Conclusion The invention describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that suppresses diagonal responses and maximizes cross-peak sensitivity, yet avoids double quantum evolution as generated by standard pulse sequences, as practiced in the prior art. The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has a superior resolution in the indirect dimension F1 than the double quantum experiments generated by standard pulse sequences, as practiced in the prior art. The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has superior sensitivity overall, than the double quantum experiments generated by standard pulse sequences, as practiced in the prior art. This arises because the relaxation of double quantum coherence during the evolution time in standard pulse sequences can lead to approximately twice as much signal loss per unit time as the relaxation of single Bruker BioSpin AG 20.06.2006 P8968IN 13 transition single quantum coherence - and in fact could be far more lossy for larger molecules (such as biomolecules) and higher magnetic filed intensities of NMR measurement. The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that permits the experimenter to deduce the spin couplings and chemical shifts, despite selecting a single transition in each 'cross-peak' multiplet, by a procedure outlined in the text. Alternatively, another embodiment of the present invention where the flip angle of the first DQ reconversion pulse is reduced, permits this information to be deduced by generating a second peak in the cross-peak multiplet with only marginal sacrifice of sensitivity, as described in the text. The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has the capabilities noted above when applied to the NMR of spin-1 systems in ordered phases (e.g. 2H, 6Li, 14N in liquid crystalline phase) and permits discrimination between isotropic and ordered phases in this application. Finally, the invention describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has the capabilities noted above when applied to NMR in the solid state with magic angle sample spinning, for example the NMR of13C, 29Si, etc. References 1. Aue, W. P.; Bartholdi, E.; Ernst, R. R. J. Chem. Phys. 1976, 64, 2229-2246. 2. Bax, A.; Freeman, R.; Kempsell, S.P. J. Am. Chem. Soc. 1980, 102, 4849- 4851. 3. Bax, A.; Freeman, R.; Frenkiel, T.A.; Levitt, M.H.; J. Magn. Reson. 1983, 43, 478-483. Bruker BioSpin AG 20.06.2006 P8968IN 14 4. Nielsen, N.C.; Thogersen, H.; S0rensen, O.W. J. Am. Chem. Soc. 1995, 117,11365-11366. 5. Nielsen, N.C.; Thogersen, H.; Sorensen, O.W. J. Chem. Phys. 1996, 105, 3962-3968. 6. Meissner, A.; Sorensen, O.W. Concept Magn. Reson. 2002, 14, 141-154. 7. Turner, D. L. Mol. Phys.1981, 44, 1051-1058. 8. Turner, D. L. J. Magn. Reson.1982, 49, 175-178. 9. Stoustrup, J.; Schedletzky, O.; Glaser, S.J.; Griesinger, C; Nielsen, N.C.; Sorensen, O.W. Phys. Rev. Lett. 1995, 74, 2921-2924. 10. Schulte-Herbruggen, T.; Madi, Z.L.; Sorensen, O.W.; Ernst, R. R. Mol. Phys.1991, 72, 847-871. 11. Glaser, S.J.; Drobny, G.P. Adv. Magn. Reson.1990, 14, 35-58. Bruker BioSpin AG 20.06.2006 P8968IN 15 Patent Claims 1. Method of 2-dimensional nuclear magnetic resonance (2D-NMR) correlation spectroscopy, comprising a pulse sequence with the following steps: i) excitation of double quantum coherence; ii) immediate reconversion to single transition single quantum coherence; iii) evolution of the set of single transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. 2. Method according to claim 1, characterized in that during step ii), a conversion to a single transition at each of the chemical shifts of two coupled spins is done. 3. Method according to any one of the preceding claims, characterized in that in step ii), the double quantum re-conversion is detuned with a first pulse having a flip angle other than 45°, preferably a reduced flip angle, in particular a flip angle of 20°-30°. 4. Method of 2D-NMR correlation spectroscopy, comprising a pulse sequence with the following steps: i) excitation of double quantum coherence; ii) immediate reconversion with a 90° pulse; iii) evolution of the set of anti-phase single quantum transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. Bruker BioSpin AG 19.06.2006 P8968IN 16 5. Method according to any one of the claims 1 through 4, characterized in that in step iv), isotropic mixing is done. 6. Method according to claim 5, characterized in that in step iv), isotropic mixing is performed with sequences optimized for chemical shift bandwidth and / or coupling bandwidth. 7. Method according to any one of the claims 1 through 4, characterized in that in step iv), a cascade of two bilinear rotations is applied. 8. Method according to claim 7, characterized in that the cascade of two bilinear rotations includes pulse contraction. 9. Method according to any one of the preceding claims, characterized in that a p pulse is applied just before or right after step iv). 10. Method according to any one of the preceding claims, characterized in that suitable offset compensated pulses or offset compensated groups of pulses are employed. 11. Method according to any one of the preceding claims, characterized in that the pulse sequence is adapted for indirect detection. 12. Method according to any one of the preceding claims, characterized in that it is used with C-13 nuclei. 13. Method according to any one of the preceding claims, characterized in that it is used with solution samples. 14. Method according to any one of the preceding claims, characterized in that it is used with solid state samples, preferably applying magic angle Bruker BioSpin AG 19.06.2006 P8968IN 17 spinning (MAS), in particular with Si-29 and/or C-13 nuclei. 15. Method according to any one of the claims 1 through 14, characterized in that it is used with rare spin-1/2 systems. 16. Method according to any one of the claims 1 through 14, characterized in that it is used with spin-1 -systems in ordered phases, in particular with H- 2, Li-6, N-14. 17. Method according to any one of the preceding claims, characterized in that the method is adapted to generate magnitude mode spectra or pure phase spectra. Bruker BioSpin AG 19.06 2006 P8968IN Dated this 4th day of JULY 2006.

Full Text

2
Such a method is known, e.g., from A. Bax et al., J. Am. Chem. Soc. 1980, 102, p. 4849-4851 (compare Ref. 2).
Nuclear magnetic resonance (NMR) is a powerful tool for investigating the chemistry of a sample. NMR measures the reaction of the spins of the nuclei within the sample upon excitation with high frequency electromagnetic pulses.
2D NMR1 techniques are especially useful to identify spin correlations which in turn reflect the bonding or spatial topology of the molecule. For low abundance spins, the correlation information has been produced by exciting Double Quantum Coherence (DQC)2,3 which is subsequently reconverted following evolution by either a single pulse (of flip angle typically 45°, 60°, 90°, 120° or 135°), or alternatively by a cluster of pulses as in the 2D INADEQUATE CR sequence4"6. 2D INADEQUATE CR has been designed to provide the maximum sensitivity and results in transition selectivity in F2. These experiments result in spectra whose F1 frequencies are sums of the chemical shifts of each pair of coupled spins. COSY- or SECSY-type displays for 13C spin systems are also known in the literature7'8. These methods afford sub-optimal sensitivity, however, there being no transition selectivity.
Object of the invention
It is the object of the invention to propose a method of 2D-NMR correlation spectroscopy which can achieve an improved sensitivity and which has a transition selectivity.
Short description of the invention
This object is achieved, in accordance with the invention, by a method of 2-dimensional nuclear magnetic resonance (2D-NMR) correlation spectroscopy, comprising a pulse sequence with the following steps:
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i) excitation of double quantum coherence;
ii) immediate reconversion to single transition single quantum
coherence;
iii) evolution of the set of single transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection. This method is further referred to as LASSY.
The object is also achieved, in accordance with the invention, by a method of 2D-NMR correlation spectroscopy, comprising a pulse sequence with the following steps:
i) excitation of double quantum coherence;
ii) immediate reconversion with a 90° pulse;
iii) evolution of the set of anti-phase single quantum transitions;
iv) mixing with zero quantum mixing Hamiltonian;
v) signal detection. This method is further referred to as LAPSY.
The basic idea of the invention, as realized in LASSY and LAPSY, is to adopt a double quantum coherence excitation and an immediate reconversion; followed by a single quantum evolution. This is in contrast to the procedures'of the state of the art, where a double quantum evolution is followed by double quantum reconversion.
The inventive approach employs a double quantum filter to discriminate against uncoupled spins, which is particularly useful for nuclei of low abundance in a sample. The subsequent conversion to single quantum coherence (SQC) introduces transition selectivity. The evolution of single quantum coherence further improves the spectral resolution and reduces relaxation losses, as compared to an evolution of DQC. In addition, with the inventive methods, diagonal peaks are suppressed.
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Preferred variants of the inventive methods
In a preferred variant of the inventive LASSY method, during step ii), a conversion to a single transition at each of the chemical shifts of two coupled spins is done. By this way, the transition selectivity can be achieved in a simple way. The selection of the desired component (high-field or low-field component of each doublet, or the so-called b or a component) may be achieved by employing suitable phases of the reconversion pulses.
In another preferred variant of the inventive LASSY method, in step ii), the double quantum re-conversion is detuned with a first pulse having a flip angle other than 45°, preferably a reduced flip angle, in particular a flip angle of 20°-30°. This facilitates the measurement of shifts and couplings.
In the following, variants of both above inventive methods (LASSY and LAPSY) are introduced.
One preferred variant of the inventive methods provides that in step iv), isotropic mixing is done. This is a simple and fast way of doing the mixing step. In particular, a GD-1 sequence can be applied.
In a further development of this variant, in step iv), isotropic mixing is performed with sequences optimized for chemical shift bandwidth and / or coupling bandwidth.
As an advantageous alternative to the above mentioned variant, in step iv), a cascade of two bilinear rotations is applied. Although taking longer, this variant avoids bandwidth vs. power deposition issues.
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Optionally, this variant may be further developed by the cascade of two bilinear rotations including pulse contraction.
Another preferred variant of the inventive method is characterized in that a p pulse is applied just before or right after step iv). This permits echo pathway selection, while transferring coherence from one doublet component in F1 to the other doublet component in F2.
Another preferred variant of the inventive methods provides that suitable offset compensated pulses or offset compensated groups of pulses are employed.
Also preferred is a variant, wherein the pulse sequence is adapted for indirect detection. Then the improvement in sensitivity is particularly evident.
In a highly preferred variant, the method is used with C-13 nuclei. C-13 can be used with the invention in natural abundance, as already practiced, avoiding any enriching of isotopes in the sample.
In a practically important variant of the inventive methods, the method is used with solution samples. Alternatively, and also relevant in practice, the method is used with solid state samples, preferably applying magic angle spinning (MAS), in particular with Si-29 and/or C-13 nuclei.
In a highly preferred variant of the inventive methods, the method is used with rare spin-1/2 systems. This variant can be applied with samples in any phase. The rarity of the spin-1/2 system guarantees single coupling between two spins-1/2. Note that according to the invention, a spin system is considered as rare if its abundance in the sample is 10% or less, and preferably 5% or less.
In an alternative, also preferred variant, the method is used with spin-1-systems in ordered phases, in particular with H-2, Li-6, N-14. The ordered phase
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guarantees quadrupolar coupling of a spin-1. This variant can be performed independent of the abundance of the spin-1 system. An ordered phase, suitable for the invention, is for example a liquid crystal. Finally, in a preferred variant, the method is adapted to generate magnitude mode spectra or pure phase spectra. Magnitude mode spectra can be produced by selecting the echo or anti-echo, employing phase cycling as described below or by using gradients. Pure phase spectra are also typically produced by using gradients or phase cycling in alternative procedures.
Further advantages can be extracted from the description and the enclosed drawing. The features mentioned above and below can be used in accordance with the invention either individually or collectively in any combination. The embodiments mentioned are not to be understood as exhaustive enumeration but rather have exemplary character for the description of the invention.
Detailed description of the invention and drawing The invention is shown in the drawing.
Fig. 1 shows schematically a 2D LASSY sequence with isotropic mixing,
in accordance with the invention;
Fig. 2 shows schematically a 2D LASSY sequence with ZQ (zero
quantum) planar mixing, in accordance with the invention;
Fig. 3 shows an experimentally obtained 2D LASSY spectrum of 0.5M
sucrose;
Fig. 4 shows a 2D LAPSY sequence with isotropic mixing, in accordance
with the invention.
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Overview
The invention proposes a new family of pulse experiments for correlation spectroscopy of low abundance spins, that results in diagonal suppression and optimal sensitivity with single transition 2D 'multiplet' structure in the cross peaks. The sequence also has better relaxation tolerance than corresponding DQ experiments. Implementation involves a double quantum filter followed by reconversion to single transition single quantum coherence, then evolution followed by isotropic mixing or a laboratory frame bilinear analog. These sequences are useful not only in 13C NMR in solution state, but also 29Si MAS NMR in the solid state and the NMR of spin-1 systems in ordered phases. An 'anti-phase' version of the experiment is also pointed out.
More specifically, this document proposes - and demonstrates for 13C at natural abundance - a family of new pulse sequences to achieve the maximum sensitivity as predicted by the relevant unitary bound9, while also achieving a diagonal suppressed COSY display with transition selectivity. F1 spectral widths are now governed by the normal single quantum spectral window, resulting in superior resolution; furthermore, since it is Single Quantum Coherence (SQC) that evolves during t1 and not DQC, relaxation losses may be expected to be typically lower per unit time, as also the single quantum linewidths.
The inventive approach
The inventive approach is based on employing a double quantum filter to discriminate against uncoupled spins, followed by conversion to single quantum coherence with transition selectivity (i.e. conversion to a single transition at each of the chemical shifts of the two coupled spins). Evolution of the set of single transitions then follows, terminated by a zero quantum mixing Hamiltonian, followed by signal detection. Echo (or anti-echo) selection is governed by the addition (or not) of a p pulse just before / right after mixing.
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Addition of the said pulse results not only in echo pathway selection, but also the selection of different multiplet components in F1 and in F2 (e.g. the low field component in F1 and the high field component in F2). As described below, this permits the spectroscopist to deduce shifts and couplings despite the selection of only one component out of the cross-peak multiplet - which should normally have four components.
We call these experiments Low Abundance Single transition correlation SpectroscopY (LASSY) and dub them CLASSY for C-13 applications.
The pulse sequences
Figure 1 illustrates a 2D LASSY sequence with isotropic mixing. Here t=(2J)-1 D =10µS. Pulse flip angles are proportional to the width of the corresponding bars: the widest represent 180° pulses, while the narrowest represents a 45° (or shorter) pulse, the intermediate width representing 90° pulses. The phase cycling is given as: j1: {4(x),4(y),4(-x),4(-y)}; j2: {4(x),4(y),4(-x),4(-y),4(-x),4(-y),4(x),4(y)}; j3: {4(x),4(y),4(-x),4(-y)}; j4: {x, -y,-x, y}; j5: {8(x, -y,-x, y), 8(-x, y, x, -y)}; j6: {-y, -x, y, x}; j7: {128( x), 128(-x)}; jR: {-x, y, x, -y, x, -y, -x, y}.
Figure 2 illustrates a 2D LASSY sequence with ZQ planar mixing. Here, t=(2J)-1,
D=10 µs. The phase cycling is given by:
j1,: {4(x),4(y)}; j2: {4(x),4(y),4(-x),4(-y)}; j3: {4(x),4(y)}; j4: {x, -y,-x,y};
j5: {4(x, -y,-x, y), 4(-x, y, x, -y)}; j6: {-y, -x, y, x};
j7: {32 (y), 32(-x), 32 (-y), 32(x)}; j8: {32 ( x), 32(y), 32 (-x), 32(-y)};
jR: {4(-X, y, x, -y, x, -y, -x, y), 4 (x, -y, -x, y, -x, y, x, -y)}.
Mixing is accomplished by a suitable zero quantum Hamiltonian, that may be generated by isotropic mixing or by a cascade of two appropriate bilinear
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rotations (which may include a pulse contraction)10. The sequence of transformations of the spin density matrix may be given as follows:

Considering the further evolution of the transverse magnetization of the first spin, we find:

In general, isotropic mixing of two spins / and S for an arbitrary duration t gives rise to:

The implementation used here includes a terminal p pulse in the case of isotropic mixing (which we currently realize with the GD-1 sequence11); this permits echo pathway selection, while transferring coherence from one doublet component in F1 to the other doublet component in F2. In the implementation with a sequence of two bilinear rotations, this may be achieved by suitably setting the phase of the last pulse relative to that of the first pulse. It may be noted that mixing with a zero quantum effective Hamiltonian effectively
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suppresses diagonal peaks, employing an appropriate mixing period x that may be chosen in light of the range of values of J in the molecule. The ratio of cross-peak to diagonal peak intensities can be shown in fact to be given by:

It may further be noted that with either of the implementations indicated above, the shifts and couplings may be retrieved from the spectrum despite transition selectivity, although in practice the accuracy is a function of the F1 resolution.
Analysis
The scalar coupling and chemical shift may be calculated as follows. The LASSY spectrum of a coupled two-spin-1/2 system has two cross peaks, each with one multiplet component. These components correspond to different coupled spin states in the two dimensions. The (F1, F2) co-ordinates of the cross-peak detected at the second spin frequency are (v1+J/2, V2-J/2), v's being chemical shift frequencies expressed in Hz. The (F1, F2) co-ordinates of the cross-peak detected at the first spin frequency on the other hand are (V2+J/2, v1-J/2). The scalar coupling may be obtained by subtracting the F2 frequency of this cross peak from the F1 frequency of the other cross peak:

The chemical shifts may now be easily obtained.
Figure 3 illustrates a 2D LASSY spectrum of 0.5M sucrose. Isotropic mixing has been implemented employing the GD-1 sequence. 64 t1 points are acquired, each with 256 scans and 5k points in t2.
Bandwidth vs. power deposition issues of isotropic mixing sequences may be avoided by employing two successive bilinear mixing sequences, although this does involve an extra mixing time of (2J)-1.
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It is noted that 'detuning' the double quantum re-conversion sequence with the first pulse having a flip angle other than 45° (say, 20°-30°) also affords near-optimum sensitivity, while permitting the measurement of shifts and couplings, since it results in cross peaks that have one strong component and one weak component, diagonally displaced with respect to each other, with the intensity ratio of-3.73: 1 (for 30° flip angle of the first re-conversion pulse).
These experiments avoid spectral registration by evolution of double quantum coherence. This has a significant consequence on resolution in F1 as well as on overall experiment sensitivity, since homonuclear double quantum coherence of rare spins in solution state is typically principally relaxed by their heteronuclear dipolar interactions with protons. Such double quantum relaxation is considerably more efficient than the relaxation of the corresponding single quantum coherence of rare spins, under extreme narrowing conditions. For larger molecular weights and/or higher magnetic field intensities, this is even more so, since the spectral density at zero frequency plays a significant role. Avoiding the evolution of DQC for ca. 100-200 ms and substituting this with DQ filtering (which lets the state of DQC persist for merely ca. 10-15 ms) could thus prove to be a major boon in biomolecular NMR as well.
The LAPSY variant
It is further noted that an alternative approach that involves excitation of DQC, followed by immediate reconversion with a 90° pulse, then spin evolution and mixing as before, also leads to diagonal suppressed correlation spectra. These spectra exhibit cross peaks, each with two multiplet components that are diagonally displaced. While this permits accurate quantification of the coupling constant, sensitivity is clearly sub-optimal (70% of the sensitivity of LASSY).
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This experiment may be termed Low abundance Anti-Phase correlation SpectroscopY (LAPSY). The 2D LAPSY pulse sequence is shown in Figure 4.
Figure 4 illustrates a 2D LAPSY sequence with Isotropic mixing. Here, x=(2J)-1, D=10 µs.
Further variants
Offset compensated versions of these experiments may be developed. The protocol may also be adapted for indirect detection. In particular, this set of experiments may also be extended to the solid state (e.g. 29Si MAS NMR) and to spin-1 systems (2H, 6Li, 14N) in ordered phases.
Conclusion
The invention describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that suppresses diagonal responses and maximizes cross-peak sensitivity, yet avoids double quantum evolution as generated by standard pulse sequences, as practiced in the prior art.
The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has a superior resolution in the indirect dimension F1 than the double quantum experiments generated by standard pulse sequences, as practiced in the prior art.
The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has superior sensitivity overall, than the double quantum experiments generated by standard pulse sequences, as practiced in the prior art. This arises because the relaxation of double quantum coherence during the evolution time in standard pulse sequences can lead to approximately twice as much signal loss per unit time as the relaxation of single
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transition single quantum coherence - and in fact could be far more lossy for larger molecules (such as biomolecules) and higher magnetic filed intensities of NMR measurement.
The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that permits the experimenter to deduce the spin couplings and chemical shifts, despite selecting a single transition in each 'cross-peak' multiplet, by a procedure outlined in the text. Alternatively, another embodiment of the present invention where the flip angle of the first DQ reconversion pulse is reduced, permits this information to be deduced by generating a second peak in the cross-peak multiplet with only marginal sacrifice of sensitivity, as described in the text.
The invention further describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has the capabilities noted above when applied to the NMR of spin-1 systems in ordered phases (e.g. 2H, 6Li, 14N in liquid crystalline phase) and permits discrimination between isotropic and ordered phases in this application.
Finally, the invention describes a novel pulse sequence for rare spin 2D NMR correlation spectroscopy, that has the capabilities noted above when applied to NMR in the solid state with magic angle sample spinning, for example the NMR of13C, 29Si, etc.
References
1. Aue, W. P.; Bartholdi, E.; Ernst, R. R. J. Chem. Phys. 1976, 64, 2229-2246.
2. Bax, A.; Freeman, R.; Kempsell, S.P. J. Am. Chem. Soc. 1980, 102, 4849-
4851.
3. Bax, A.; Freeman, R.; Frenkiel, T.A.; Levitt, M.H.; J. Magn. Reson. 1983,
43, 478-483.
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4. Nielsen, N.C.; Thogersen, H.; S0rensen, O.W. J. Am. Chem. Soc. 1995,
117,11365-11366.
5. Nielsen, N.C.; Thogersen, H.; Sorensen, O.W. J. Chem. Phys. 1996, 105,
3962-3968.
6. Meissner, A.; Sorensen, O.W. Concept Magn. Reson. 2002, 14, 141-154.
7. Turner, D. L. Mol. Phys.1981, 44, 1051-1058.
8. Turner, D. L. J. Magn. Reson.1982, 49, 175-178.
9. Stoustrup, J.; Schedletzky, O.; Glaser, S.J.; Griesinger, C; Nielsen, N.C.;
Sorensen, O.W. Phys. Rev. Lett. 1995, 74, 2921-2924.

10. Schulte-Herbruggen, T.; Madi, Z.L.; Sorensen, O.W.; Ernst, R. R. Mol.
Phys.1991, 72, 847-871.
11. Glaser, S.J.; Drobny, G.P. Adv. Magn. Reson.1990, 14, 35-58.
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Patent Claims
1. Method of 2-dimensional nuclear magnetic resonance (2D-NMR)
correlation spectroscopy, comprising a pulse sequence with the following
steps:
i) excitation of double quantum coherence;
ii) immediate reconversion to single transition single quantum
coherence;
iii) evolution of the set of single transitions; iv) mixing with zero quantum mixing Hamiltonian; v) signal detection.
2. Method according to claim 1, characterized in that during step ii), a
conversion to a single transition at each of the chemical shifts of two
coupled spins is done.
3. Method according to any one of the preceding claims, characterized in
that in step ii), the double quantum re-conversion is detuned with a first
pulse having a flip angle other than 45°, preferably a reduced flip angle,
in particular a flip angle of 20°-30°.
4. Method of 2D-NMR correlation spectroscopy, comprising a pulse
sequence with the following steps:
i) excitation of double quantum coherence;
ii) immediate reconversion with a 90° pulse;
iii) evolution of the set of anti-phase single quantum transitions;
iv) mixing with zero quantum mixing Hamiltonian;
v) signal detection.
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5. Method according to any one of the claims 1 through 4, characterized in
that in step iv), isotropic mixing is done.
6. Method according to claim 5, characterized in that in step iv), isotropic
mixing is performed with sequences optimized for chemical shift
bandwidth and / or coupling bandwidth.
7. Method according to any one of the claims 1 through 4, characterized in
that in step iv), a cascade of two bilinear rotations is applied.
8. Method according to claim 7, characterized in that the cascade of two
bilinear rotations includes pulse contraction.
9. Method according to any one of the preceding claims, characterized in
that a p pulse is applied just before or right after step iv).
10. Method according to any one of the preceding claims, characterized in
that suitable offset compensated pulses or offset compensated groups of
pulses are employed.
11. Method according to any one of the preceding claims, characterized in
that the pulse sequence is adapted for indirect detection.
12. Method according to any one of the preceding claims, characterized in
that it is used with C-13 nuclei.
13. Method according to any one of the preceding claims, characterized in
that it is used with solution samples.
14. Method according to any one of the preceding claims, characterized in
that it is used with solid state samples, preferably applying magic angle
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spinning (MAS), in particular with Si-29 and/or C-13 nuclei.
15. Method according to any one of the claims 1 through 14, characterized in
that it is used with rare spin-1/2 systems.
16. Method according to any one of the claims 1 through 14, characterized in
that it is used with spin-1 -systems in ordered phases, in particular with H-
2, Li-6, N-14.
17. Method according to any one of the preceding claims, characterized in
that the method is adapted to generate magnitude mode spectra or pure
phase spectra.
Bruker BioSpin AG 19.06 2006 P8968IN
Dated this 4th day of JULY 2006.

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Patent Number

259545

Indian Patent Application Number

669/KOL/2006

PG Journal Number

12/2014

Publication Date

21-Mar-2014

Grant Date

17-Mar-2014

Date of Filing

04-Jul-2006

Name of Patentee

BRUKER BIOSPIN AG

Applicant Address

INDUSTRIE STRASSE 26, CH-8117 FALLANDEN

Inventors:

#	Inventor's Name	Inventor's Address
1	NARAYANAN CHANDRAKUMAR	H4-2, SOUTHERN SHELTERS, KATTUPAKKAM CHENNAI 600056
2	VELUSAMY RAMESH	S/O P.C. VELUSAMY VELLIAMPALAYM AYYAMPALAYAM POST KODUMUDI (VIA) 638151

PCT International Classification Number

N/A

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA