Title of Invention

ANTI-TRKA ANTIBODY

Abstract

Antibodies and derivatives thereof An anti-TrkA antibody is provided that comprises: a) a variable heavy chain comprising a sequence selected from any of BXhVHl, BXhVH2, BXhVH3, BXhVH4, BXhVH5, or HuVHWOv as shown in Figure la; or from variants of any of said sequences and/or b) a variable light chain comprising a sequence selected from any of BXhVLl, BXhVL2, BXhVL3, BXhVL4, BXhVL5, BXhVL6, BXhVL7 or BXhVL8; as shown in Figure lb, or from variants of any of said sequences. TrkA-binding derivatives are also provided. Antibodies or derivatives of the present invention are useful in a number of therapies, including pain therapy.

Full Text

FORM 2 THE PATENTS ACT 1970 (Act 39 of 1970) COMPLETE SPECIFICATION (SECTION 10) ANTI-TRKA ANTIBODIES AND DERIVATIVES THEREOF Lay Line Genomics S.p.A Via Prospero Colonna32, IT-00149 Roma, Italy THE FOLLOWING SPECIFICATION DESCRIBES THE NATURE OF THE INVENTION; AND THE MANNER IN, WHICH IT IS TO BE PERFORMED Antibodies and derivatives thereof The present invention relates to antibodies and to derivatives thereof, especially to humanised antibodies and derivatives thereof. Nerve growth factor (NGF) acts through two membrane receptors. One is the relatively low affinity p75 receptor. The other is a 140 KDa high affinity receptor, known as TrkA. NGF has potential use in the treatment of a wide range of disorders, such as various neurodegenerative disorders (including Alzheimer's disease), diabetes and leprosy. However NGF can have various undesired agonist properties. These include an increase in pain sensitivity. The NGF-TrkA system provides a potential target for therapies for pain. Various anti-TrkA antibodies have been produced. One such antibody is a monoclonal antibody which is referred to as 5C3 in WO 97/21732 (McGill University). However, this was found to be a TrkA agonist and is therefore not useful for reducing pain. Specifically, when binding to TrkA this antibody does not prevent the functional activation thereof. An anti-TrkA monoclonal antibody known as MNAC13 is disclosed in WO 00/73344 (Societa Italiana Per La Ricerca Scientifica), from which EP-B-118138 (Lay Line Genomics SpA) is derived. This antibody and various derivatives thereof are said to be effective in preventing the functional activation of TrkA in a range of biological systems. The MNAC13 monoclonal antibody was used in a standard nociception test and was found to provide remarkable hypoalgesia. A single chain Fv (ScFv) variant of this antibody is also disclosed in WO 00/73344 and is referred to therein as MNAC13 ScFv. This contains the variable light and heavy chain regions of the larger antibody linked together by a linker polypeptide, which joins the C-terminus of the VL region with the N-terminus of the VH region. This variant was found to bind TrkA as efficiently as MNAC13. The sequence of the light and heavy variable regions was compared with that of the corresponding regions of the antibody described in WO 97/21732 and it was found that there was only a low level of overall sequence identity therewith. WO 06/131952 (Lay Line Genomics SpA) discloses medical uses of anti-TrkA antibodies in treating chronic pain. It provides evidence of this by using models of persistent pain, in particular the Chronic Constriction Injury (CCI) model. 'WO 06/1371066 [Lay Line Genomics SpA) discloses using an anti-Tr'KA antibody capable of inhibiting the binding between NGF and TrkA in combination with at least one opioid analgesic for treating or preventing pain. It is explained that this combination therapy allows a reduced opioid dosage to provide the same level of pain relief as a much higher dosage. This can therefore be useful in reducing the level of opioid side effects in pain therapy, because dosages can be lowered. WO 05/061540 (Lay Line Genomics SpA & Scuolo Internazionale Superiore Di Studi Avanzati-Sissa) discloses a method of humanisation of antibodies in which structural data obtained from crystal lographic studies are used to conduct the first design stages of humanisation. As examples, WO 05/061540 takes anti-TrkA antibodies, as disclosed in WO 00/73344, and anti-NGF antibodies as starting points, and then redesigns them using the disclosed method. Whilst the humanised antibodies disclosed in WO 05/061540 are useful, there is a need to provide additional humanised antibodies so as to expand the possibilities for effective therapies. The present inventors have now provided a range of anti-TrkA antibodies and derivatives thereof that are not disclosed in WO 05/061540. The inventors have also provided data indicating the utility of such antibodies. Prior to the present invention these antibodies were simply not known in the art and the data provided could not have been predicted. According to one aspect of the present invention, there is provided an anti-TrkA antibody that comprises: a) a variable heavy chain comprising a sequence selected from any of BXhVHl (SEQ ID NO 1), BXhVH2 (SEQ ID NO 2), BXhVH3 (SEQ ID NO 3), BXhVH4 (SEQ ID NO 4).. BXhVH5 (SEQ ID NO 5), or HuVHWOv (SEQ ID NO 6), as shown in Figure la; or from variants of any of said sequences; and/or b) a variable light chain comprising a sequence selected from any of BXhVLl (SEQ ID NO 7), BXhVL2 (SEQ ID NO 8), BXhVL3 (SEQ ID NO 9), BXhVL4 (SEQ ID NO 10), BXhVL5 (SEQ ID NO 11), BXhVL6 (SEQ ID NO 12), BXhVL7 (SEQ ID NO 13) or BXhVL8 (SEQ ID NO 14); as shown in Figure lb, or from variants of any of said sequences. A derivative of said antibody is also provided; wherein the derivative is capable of binding TrkA. More preferably, the antibody comprises both a variable heavy chain as described in a) above and a variable light chain as described in part b), i.e. it comprises one of the following combinations of light and heavy chains: BXhVHIVLl, BXhVHlVL2, BXhVHlVL3, BXhVHlVL4, BXhVHlVL5, BXhVHlVL6, BXhVHlVL7, BXhVHlVL8; BXhVH2VLl, BXhVH2VL2, BXhVH2VL3, BXhVH2VL4, BXhVH2VL5, BXhVH2VL6, BXhVH2VL7, BXhVH2VL8, BXhVH3VLl, BXhVH3VL2, BXhVH3VL3, BXhVH3VL4, BXhVH3VL5, BXhVH3VL6, BXhVH3VL7, BXhVH3VL8, BXhVH4VLl, BXhVH4VL2, BXhVH4VL3, BXhVH4VL4, BXhVH4VL5, BXhVH4VL6, BXWH4VL7, BXhVH4VL8, BXhVH5VLl, BXhVH5VL2, BXhVH5VL3, BXhVH5VL4, BXhVH5VL5, BXhVH5VL6, BXhVH5VL7, BXhVH5VL8, or HuVHWOv/HuVLWO. Desirably, the derivative of the antibody has at least one CDR region selected from the regions underlined in Figures la & 1 b for each sequence, or from variants thereof having no more than two amino acid changes (preferably no more than one amino acid change) per underlined region. More desirably, it has a plurality of CDR regions selected from the regions underlined in Figures la & lb for each sequence, or from variants thereof having no more than two amino acid changes (preferably no more than one amino acid change) per underlined region. It may therefore comprise one, two, three, four, five or six of such CDR regions (optionally in combination with one or more other CDR regions). It may preferably comprise at least the third CDR region of the heavychain, more preferably at least the third CDR region of the heavy and light chains. i Most desirably, however, it has six CDR regions corresponding to the six CDR regions underlined in Figures la & lb for each sequence or corresponding to variants thereof having no more than two amino acid changes per underlined region. Indeed, in most cases, it is preferred that few or no changes are made to the CDR sequences. Thus one, two, three, four, five, or even all six CDR regions may have the same amino acid sequences as those shown in Figures la & lb. Turning now to framework regions, it is preferred that the derivative has at least one framework region selected from the non-underlined sequences shown in Figures la & lb or from variants thereof that have at least 75% amino acid sequence identity therewith (e.g. at least 80%, at least 85%, at least 90%, at least 95% or at least 98% sequence identity therewith). The degree of amino acid sequence identity can be determined by simple alignments of the sequences without any gaps and determining the sequence differences. Sequences can be aligned according to Kabat's numbering scheme and sequence identities can then be determined accordingly (See Kabat, Sequences of Proteins of Immunological Interest, National Institutes of Health, Bethesda MD, 1987 & 1991). This numbering scheme is discussed in WO 05/061540 (Reference can also be made to Chothia & Lesk, J. Mol. Biol., 196, 901 (1987) and to Chothia et al., Nature, 342, 878 (1989)). Less preferably, one or more gaps may be allowed (e.g. for one or more amino acid insertions/deletions) and gap penalties may then be assigned. Sequence identity can be determined using sequence analysis software e. g., BLASTN or BLASTP (available at www. ncbi.nlm.nih.gov/BLAST/). The default parameters for comparing two sequences (e.g. "Blast' ing two sequences against each other) by BLASTN (for nucleotide sequences) are reward for match = 1, penalty for mismatch = 2, open gap = 5, extension gap = 2. When using BLASTP for protein sequences, the default parameters are reward for match = 0, penalty for mismatch = 0, open gap =11, and extension gap = 1]. More preferably, a plurality of framework regions is present and these regions are selected from the non-underlined sequences shown in Figure la & lb or from variants thereof that have at least 75% amino acid sequence identity therewith (e.g. at least 80%, at least 85%, at least 90%, at least 95% or at least 98% sequence identity. Each chain shown in Figure la & lb has four framework regions. Thus it is preferred that at least two, at least three or four such regions/variants thereof are present. Most preferably, all four framework regions or variants thereof are present. Where one or more variant framework regions are present, it is generally preferred that the these regions do not include amino acid substitutions that would result in a change to an amino acid that is present in a murine sequence at the corresponding position. The relevant murine amino acids that can be used for comparison are shown in mVHEP and mVLEP in Figures la & lb respectively, with the exception that, for the purposes of this discussion, the few italicised amino acids shown in mVHEP and mVLEP are considered to be non-murine. At these positions the residues considered to be murine are given in the table below, in the order in which the italicised residues appear in the Figures. Position Italicised residue shown in Figure Corresponding murine residue Heavy chain M V Heavy chain Q G Light Chain D Q Light Chain S T Thus the percentage of humanisation of one or more framework regions may be reduced by amino acids substitutions that do not necessarily increase the percentage of murine residues present. These may result from conservative non-murine amino acid substitutions and/or from non-conservative non-murine substitutions. However conservative substitutions are most preferred. Amino acids can be grouped as follows: Group I (hydrophobic lateral chains): M, A, V, L, I; Group II (neutral hydrophilic lateral chains): C, S, T, N, Q; Group III (acid lateral chains): D, E; Group IV (basic lateral chains): K, R; Group V (residues that influence the orientation of the main chain): G, P; and Group VI (aromatic lateral chains): F, Y, W. Conservative amino acid substitutions entail substitutions between amino acid of the same group, whilst non conservative amino acid substitutions entail an exchange between members of different groups. Whatever sequences are present in the different regions of the light and/or heavy chains, it is preferred that an antibody or derivative of the present invention has certain functional characteristics. In addition to binding to TrkA, it is preferred that an antibody or derivative of the present invention is capable of blocking or reducing the binding of NGF to TrkA. Preferably, it is capable of blocking or reducing one or more biological activities that would otherwise be induced by the binding of NGF to the TrkA receptor. Thus it is preferred that it is an antagonist of one or more activities induced by NGF binding to TrkA (rather than an agonist). Thus the antibodies and derivatives thereof according to the invention suitably prevent the functional activation of TrkA. Inhibition of functional activation of TrkA by antibodies and derivatives thereof can lead to analgesia in vivo. Various assay procedures can be used. A standard assay is the classical PC 12 in vitro assay in which PC 12 cells are incubated with NGF and candidates are assessed to see if they are effective in reducing the extension of NGF-induced neuritic growth. This model was used in WO 00/73344, for example. In another assay, preferred antibodies produce an OD450/630 nm value of greater than 0.1 in the TrkA-IgG binding assay illustrated by Figure 2. More preferably the OD450/630 nm value is greater than 0.2. Most preferably it is greater than 0.3. In a further assay, preferred antibodies or derivatives thereof provide an increase in FACS staining of TF1 cells in the FACS based assay described in the Examples (see Table 2). This is preferably an increase of over 1.0 fold. More preferably it is an increase that is at least 1.5 fold, at least 2.0 fold or at least 2.5 fold. Most preferably it is at least 3.0 fold. Additional assays include assays for pain reduction, as described later in connection with the medical uses of the present invention (It is particularly desirable for medical applications that the antibodies/derivatives thereof act as antagonists rather than agonists in respect of the pain response). Desired antibodies/derivatives of the present invention are selective in that they bind with greater affinity to TrkA than to TrkB (Compare the black and white columns in Figure 2, for example). For example they preferably have a binding affinity that is at least 2 times, at least 4 times, or at least 6 times as great for TrkA than for TrkB. High binding affinities to TrkA relative to TrkB result in greater selectivity and a lower risk of undesired side effects. Binding affinities can be readily assayed by comparative binding studies, such as those illustrated in Figure 2. Turning now to highly preferred antibodies of the present invention, these comprise one of the following combinations of light and heavy chains: BXhVH3VL3, BXhVH5VL1 or BXhVH5VL3. These gave the best results in the assay illustrated by Figure 3. Preferred derivatives are derivatives of BXhVH3VL3, BXhVH5VLl or BXhVH5VL3. It will be appreciated from the foregoing discussion that a wide range of antibodies and derivatives thereof are within the scope of the present invention. These have numerous applications, including those discussed below: Medical applications Antibodies or derivatives of the present invention can be used in medicine. They can be used to treat various disorders/conditions, as set out in various categories below. The invention thus provides a method of treatment of the below mentioned conditions which comprises administering to a subject, suitably a mammalian subject especially a human subject, in need thereof a therapeutically effective amount of an antibody or derivative as described herein such that the condition is thereby treated. The invention also provides use of an antibody or derivative as described herein in the manufacture of a medicament for the treatment of the below mentioned conditions. The invention also provides a kit of parts comprising an antibody or derivative as described herein together with instructions directing the use thereof by a subject for the treatment of the below mentioned conditions. Here the term "treatment" includes therapeutic treatment of an existing disorder/condition. It also includes prophylactic treatment. It further includes the amelioration of one or more adverse symptoms, even if a patient is not cured of a given disorder/condition. For example, pain may be alleviated or reduced. Pain A preferred medical use is in the treatment of pain. According to International Association for the Study of Pain ("IASP") pain is generally defined as "An unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage or both". The essential element in all forms of pain is the activation of specialized high-threshold receptors and nerve fibers to warn the organism of potential tissue damage. The involvement of inflammatory cells and processes is a common element in many pain states. The term "acute pain" means immediate, generally high threshold, pain brought about by injury such as a cut, crush, burn, or by chemical stimulation. The term "chronic pain," as used herein, means pain other than acute pain, both of inflammatory and neuropathic origin. It is understood that chronic pain often is of relatively long duration, for example, months or years and can be continuous or intermittent. Antibodies of the present invention can be used to treat chronic pain or acute pain. The treatment of chronic pain is preferred The use of anti-TrkA antibodies in treating pain is discussed in WO 00/73344, in WO 05/061540 and in WO 06/131952 for example. The pain may for example be associated with any of the following: pancreatitis, kidney stones, endometriosis, IBD, Crohn's disease, post surgical adhesions , gall bladder stones, headaches, dysmenorrhea, musculoskeletal pain, sprains, visceral pain, ovarian cysts, prostatitis, cystitis, interstitial cystitis, post-operative pain, migraine, trigeminal neuralgia, pain from burns and/or wounds, pain associated with trauma, neuropathic pain, pain associated with musculoskeletal diseases, rheumatoid arthritis, osteoarthritis, ankylosing spondilitis, periarticular pathologies, oncological pain, pain from bone metastases, HIV infection. Various models are known for assessing pain and can be used in screening antibodies/derivatives thereof. For example, the nociception hot plate test can be used, as disclosed in WO 00/73344, for example. The experiment can be carried out according to McMahon et al., Nature Medicine, 1, 774-780 (1995), using the antibody/derivative as immunoadhesin. The antibody/derivative is infused subcutaneously into hind paw of an adult rat for a period of three weeks or by an osmotic mini-pump. The nociception sensitivity is evaluated at intervals using a hot plate test (Eddy and Leimbach, J. Phar. Exp. Ther., 107, 385-393(1953)). which mimics hyperalgesia situations following inflammation or partial damage to the nerve. The nociceptive stimulus induces in such a case a response (paw licking and/or jumping) which presumes an integrated coordination higher than simple reflex. According to the test the animal is put in a pen having a plate heated to the desired temperature as base, usually 56°C. The latency of any of two responses (paw licking and jumping) is measured in control animals (treated with non relevant antibody) and in those treated with the anti-TrkA antibody/derivative. As an alternative to the hot plate test, the nociceptive response to formalin can be assessed. This test is disclosed by Porro and Cavazzuti in Prog. Neurobiol., 41:565- 607 (1993) and was used in WO 06/137106. It involves assessing the reduction in pain response by analyzing any subsequent reduction in paw licking when a given candidate is administered prior to testing. Saline is typically used as a negative control. The Chronic Constriction Injury (CCI) model is also a well known animal model. It involves chronic constriction of the sciatic nerve and is used for assessment of chronic pain of a neuropathic nature. This model is described by Bennett and Xie in Pain, 33, 87-107 (1988). It was used in WO 06/131592, for example. Cancer The antibodies/derivatives can also be used in the treatment of cancer. Various cancers express TrkA. The interaction of TrkA with NGF may be involved in tumour development (e.g. of prostate and pancreatic cancers). Indeed in certain forms of cancer, an excess of NGF can facilitate the growth and infiltration of nerve fibres. By blocking the action of NGF it is possible to significantly reduce the formation of neuromas. Furthermore, as an alternative to simply providing a blocking effect, the antibodies/derivatives can be coupled to a cytotoxic agent and can be used to target cancer cells expressing TrkA, as discussed later in further detail. It is not however necessary to couple the antibodies/derivatives to toxins. ADCC (antibody-dependent cell-mediated cytotoxicity) arises due to an immune response in which antibodies/derivatives, by coating target cells, can make them vulnerable to attack by the system (e.g. by T cells, by complement activation, etc.) Neuronal disorders The antibodies/derivatives can also be used in the treatment of various neuronal disorders. As indicated above the antibodies/derivatives can be used to reduce the formation of neuromas. They can also be used in the treatment of neurodegenerative disorders. As discussed earlier, NGF has potential use in the treatment of Alzheimer's disease, but has undesired agonist properties, including an increase in pain sensitivity. Antibodies/derivatives of the present invention may be useful in such treatments to reduce undesired agonist effects of NGF (see also the "Combination therapy" section below). Furthermore, the antibodies/derivatives can be used to treat neuropathic pain, as discussed above. This may be associated with a lesion or a dysfunction of the nervous system. Inflammatory Disorders A still further application is in the treatment of inflammatory disorders. NGF is released by mast cells, fibroblasts and other cell types in the peripheral sites where inflammatory processes occur. In particular, mast cells appear to play a fundamental role. They produce NGF and at the same time express functional TrkA receptors at their surface. The NGF/TrkA system appears to mediate mastocyte activation through an autocrine positive feedback mechanism which allows local amplification of the algogenic inflammatory signal. Examples of inflammatory disorders that may be treated include inflammatory forms of the urinary tract and of the pelvic region, osteoarthritis, rheumatioid arthritis, asthma. Other disorders As discussed earlier, NGF has potential use in the treatment of diabetes and leprosy, but has undesired agonist properties, including an increase in pain sensitivity. Antibodies/derivatives of the present invention may be useful in such treatments to reduce undesired agonist effects of NGF (see also the "Combination therapy" section below). Combination therapy Antibodies or derivatives thereof of the present invention may be used together with one or more other active agents in combination therapy. They may be used for simultaneous, sequential or concerted administration in medicine. For example, the antibody or derivative may be combined with an analgesic opioid. WO 06/137106 explains that small amounts of molecules able to block TrkA biological activity can potentiate the analgesic effects of opioids. Such opioids include one or more compounds selected from the following: morphine, codeine, dihydrocodeine diacetylmorphine, hydrocodone, hydomorphone, levorphanol, oxymorphone, alfentanil, buprenorphine, butorphanol, fentanyl, sufentanyl, meperidine, methadone, nabulfina, propoxyphene, pentazocine, and their pharmaceutical ly acceptable derivatives thereof (e.g. pharmaceutically acceptable salts thereof). Alternatively, the antibody or derivative may be used in combination therapy with one or more non-opioid analgesic. 16 HU SEP 2010 A further combination is that of the antibody or derivative with NGF. As discussed above, the use of NGF in the treatment of various disorders, including Alzheimer's disease, diabetes mellitus, leprosy, etc., had been proposed, but increases in pain sensitivity had been noted arising from agonist properties towards peripheral targets. Again, by using an antibody or derivative of the present invention, pain sensitivity can be reduced, thereby making NGF-based therapies more attractive. A further combination is that of one or more antibodies or derivatives of the present invention together with one or more other antibodies. A preferred combination is with one or more other anti-TrkA or anti-NGF antibodies. Such combinations may provide increased efficacy in treating one or more of the disorders discussed herein, relative to treatment with a single antibody. For example combinations of two or more antibodies found to be amongst the most effective in assay procedures used herein may be used. Pharmaceutical compositions, vehicles and routes of administration The antibodies/derivatives of the present invention can be administered by any appropriate route. This includes (but is not limited to) intraperitoneal, intramuscular, intravenous, subcutaneous, intratracheal, oral, enteral, parenteral, intranasal or dermal administration. Thus the invention provides a pharmaceutical composition comprising an antibody or derivative thereof together with a pharmaceutically acceptable carrier or excipient. The antibodies/derivatives can typically be administered for local application by injection (intraperitoneal or intracranial-typically in a cerebral ventricle-or intrapericardiac or intrabursal) of liquid formulations or by ingestion of solid 17 [- 1 SEP Z010 formulations (in the form of pills, tablets, capsules) or of liquid formulations (in the form of emulsions and solutions). Compositions for parenteral administration commonly comprise a solution of immunoglobulin dissolved in a compatible, preferably aqueous solution. The concentration of the antibody/derivative in these formulations can vary from less than 0.005% to 15-20% w/v. It is selected mainly according to the volumes of the liquid, viscosity, etc, and according to the particular administration mode desired. Alternatively, the antibodies/derivatives can be prepared for administration in solid form. The antibodies can be combined with different inert or excipient substances, which can include ligands such as microcrystalline cellulose, gelatin or Arabic rubber; recipients such as lactose or starch; agents such as alginic acid, Primogel or corn starch; lubricants such as magnesium stearate, colloidal silicon dioxide; sweeteners such as saccharose or saccharin; or flavours, such as mint and methyl salicylate. Other pharmaceutical administration systems include hydrogel, hydroxymethylcellulose, liposomes, microcapsules, microemulsions, microspheres, etc. Local injections directly at a site affected by a disorder /close thereto is a preferred mode of administration if a disorder is localised. In contrast to anti-tumour based therapies, WO 06/131952 discusses the use of various anti-TrkA antibodies in the treatment of pain. Here it is explained that anti-TrkA antibodies are suitably administered systemically. Systemic administration can be performed by injection, e.g. continuous intravenous infusion, bolus intravenous infusion, subcutaneous or intramuscular injection. Alternatively, other forms of administration (e.g. oral, mucosal, via inhalation, sublingually, etc.) may also be used. If desired, however, delivery of the antibody/ derivative can be performed by local administration (e.g. intra-articular injection or subcutaneous, intramuscular injection) in the vicinity of affected tissues. The anti-TrkA antibody/derivative will suitably be formulated in a pharmaceutical composition appropriate for the intended route of administration. Solutions for injection will suitably contain the antibody/derivative dissolved or dispersed in an aqueous medium (e.g. water for injection) as appropriate containing appropriate buffers and molarity modifiers (e.g. phosphate, salt and/or dextrose). The treatment regime (i.e. dose, timing and repetition), can be represented by single or repeated administrations (e.g. injections) of the product by the chosen administration route. The interval of dose administration can be subject to modifications depending on the extent and duration of the clinical response, as well as the particular individual and the individual clinical history. Suitably the anti-TrkA antibody/derivative has a long duration of action. In particular the clinical effect of the antibody following administration may be as long as 21 days as determined from animal studies. Furthermore, anti-TrkA antibodies/derivatives may manifest clinical benefit for a longer period than that in which its presence can be detected in a relevant biological matrix such as serum or plasma following its administration. In light of the intended long duration of action (i.e. an effect suitably lasting at least one week, or preferably at least two weeks e.g. at least three weeks or at least four weeks), suitably the antibody/derivative may be administered to subjects at a frequency of not more than once per week e.g. not more than once per two weeks or once per three weeks or once per four weeks. A suitable daily dose of the anti-TrkA antibody/derivative will typically range from 0.1 5 mg/kg to 10 mg/kg body weight. (Using humanised anti-TrkA antibodies and a CCI model it is reported in WO 06/131592 that significant analgesic properties were observed in experimental animals at a dosage of 2 mg/kg, although lower dosages may of course be preferred for humans.) Turning now to administration in respect of tumours, administration may be through direct and localized injection into a tumour or a tissue near the tumour site. For systemic administration, doses vary from 0.05 mg/kg per day to 500 mg/kg per day, although dosages in the lower region of the range are preferred because they are easier to administer. Dosages can be calibrated for example to guarantee a particular level in the plasma of the antibody/derivative (in the range of about 5-30 mg/ml, preferably between 10-15 mg/ml) and maintain this level for a given period of time until the clinical results are achieved. Effective methods for measuring or assessing the stage of pancreatic or prostatic tumours are based on the measurement of the prostate specific antigen (PSA) in blood, on the measurement of the survival time for pancreas tumours, on the measurement of the slowing or inhibition of diffusion for metastases in the case of both tumour types. For direct injection at the level of a tumour site, dosage depends on different factors including the type, stage and volume of the tumour, along with many other variables. Depending on tumour volume, typical therapeutic doses may vary from 0.01 mg/ml to 10 mg/ml injections which can be administered with the necessary frequency. Whatever the nature of the therapy, humanised antibodies/derivatives may be eliminated much more slowly and require lower dosages to maintain an effective level in the plasma than non-humanised antibodies. Moreover, with high affinity antibodies/derivatives, administration may be less frequent and less sizable than with antibodies having lower affinity. The therapeutically effective dosage of each antibody/ derivative can be determined during the treatment by a skilled medical practitioner. If necessary, dosages can be reduced (e.g. to reduce side effects) or increased (to increase activity). Prior to administration, preparations of antibodies/derivatives of the invention can be stored by being frozen or lyophilized. They may then be reconstituted immediately before use in a suitable buffer. Given that lyophilisation and reconstitution can result in a loss in activity, antibody administration levels can be calibrated to compensate for this fact. (For conventional immunoglobulins, IgM antibodies tend to have a greater loss of activity than IgG antibodies). A shelf life may also be assigned so that antibodies/derivatives are not used after a certain period of storage. Diagnostic and prognostic applications An antibody or derivative thereof of the present invention can be used in the diagnosis or prognosis of any of the diseases/ conditions discussed above in relation to medical uses. For example it may be used to facilitate detection of TrkA positive tumour markers, as a precocious marker of the insurgence of Alzheimer's disease, etc. It may also be used in the diagnosis of CIPA ("congenital insensitivity to pain with anhydrosis"). This is a hereditary, recessive, autosomal syndrome characterised by recurrent episodic fever, anhydrosis, the absence of reaction to nociceptive stimuli, mental retardation and a tendency to self-mutilation. It results from mutations in the TrkA gene. Indeed an antibody or derivative of the present invention may be used in the diagnosis or prognosis of a wide range of conditions involving aberrant expression of TrkA (compared to expression of TrkA in a healthy individual or a healthy tissue sample). The present invention therefore includes within its scope a method comprising obtaining a biological sample obtained from a patient and contacting the sample with an antibody or derivative of the present invention. If desired, the antibody/derivative may be immobilised. It may be provided in the form of a diagnostic kit. The method may then include assaying the binding of the antibody / derivative to said sample in a quantitative or qualitative manner. If desired, this may be done with reference and/or to a positive control (indicating a healthy state) or a negative control (indicating the presence/likelihood of a disorder), For diagnostic purposes, the antibodies/derivatives can be both marked with a detectable marker or can be unmarked. (The term "marker" is used herein to include labels or any other detectable moiety/moiety that can trigger a detectable change.) Unmarked antibodies can be used in combination with other marked antibodies (secondary antibodies), which are reactive against humanised, or human antibodies (e. g. specific antibodies for the constant regions of human immunoglobulins). Alternatively, antibodies can be marked directly. A wide variety of markers can be used, e. g. radionuclides, fluorophores, colourings, enzymes, enzymatic substrates, enzymatic factors, enzymatic inhibitors, ligands, etc. In particular, for diagnostic or prognostic imaging applications, a detectable agent is conjugated to the antibody that is detectable or marked with a detectable radioisotope (e.g. a radioisotope such as of iodine, indium, technetium) or in paramagnetic manner (with paramagnetic atoms or ions, such as transition elements, actinides and rare earths, in particular, manganese II, copper II and cobalt II). Imaging procedures may entail the intravenous, intraperitoneal or subcutaneous injection (in lymphatic drainage regions to identify lymph node metastases) and may use detectors of radionuclide emissions (such as scintillation β counters) in the case of immunoscintigraphy. If a paramagnetic marking is used instead, an NMR spectrometer can be used. Other applications The antibodies/derivatives thereof may be used as starting points to develop further antibodies. Thus they may be used as design tools. They may be screened by one or more binding/functional assays and may therefore be part of a drug development program. They may be used for tissue typing, for forensic studies, etc. They may be used as research tools For example they may be used for further research into TrkA and/or into disorders in which TrkA binding to NGF (or other TrkA binding agents) may be implicated. They may be used to study binding and/or activation All of the above applications of the antibodies/derivatives are within the scope of the present invention. Nature of antibodies and antibody derivatives It will be appreciated from the foregoing description that a wide range of antibodies and derivatives thereof can be used in the present invention. For the avoidance of doubt the terms "antibodies" and "antibody derivatives" are discussed below in further detail. Antibodies Antibodies of the present invention can be in the form of any desired immunoglobulin structure. IgG and IgM are however preferred, with IgG being the most preferred. Of the IgG isoforms, IgGl is preferred, but other forms can be used including IgG4. The antibodies are chimeric, i.e. they include one or more regions that are normally not associated with one another in nature. More specifically, one or more murine-derived CDR regions are present in the antibodies, but other regions (especially constant regions) are preferably human or humanised. Humanised regions have more residues in common with a given human immunoglobulin region than with a corresponding mouse immunoglobulin region. Preferably, they have at least 75%, at least 80%, at least 85%, at least 90%, at least 95% or at least 98% identical with the human region at the amino acid sequence level. More preferably, there is 100% sequence identity over one or more non-CDR regions (e.g. constant regions). In some cases, however it may be beneficial to introduce certain changes. For example, it may be desirable to introduce changes that prevent/reduce one or more of the following: a) activation of the complement system b) complement mediated lysis c) activation of T cells d) binding to an Fc receptor. Mutations indicated to allow one more of the above to be achieved are discussed in various patents. One or more of said mutations may therefore be included in antibodies/derivatives of the present invention For example, US Patent No 6,194,551 proposes amino acid substitutions at amino acid positions 322, 329 and/or 331 (using the Kabat numbering system) of the constant heavy chain region of the IgG molecule and suggests that they can be used to prevent/reduce undesired activation of the complement system by abolishing Fc binding to Clq (see also Ward and Ghetie. Therapeutic Immunology 2: 77-94 (1995)). US Patent No 6,194,551 explains that proline is conserved at position 329 in human IgG's. This residue (which is glycosylated and may thereby be involved in activating the complement system) is preferably replaced with alanine. However, substitution with any other amino acid is contemplated, e.g., serine, threonine, asparagine, glycine or valine. US Patent No 6,194,551 explains that proline is also conserved at position 331 in human IgGl, IgG2 and IgG3, but not IgG4 (which has a serine residue at position 331). Residue 331 is preferably replaced by alanine or another amino acid, e.g. serine (for IgG regions other than IgG4), glycine or valine. A further possibility discussed is to introduce substitutions at position 322. Lysine 322 is conserved in human IgGs, and this residue is said to be preferably replaced by an alanine residue, although a substitution with any other amino acid residue is contemplated (e.g. serine, threonine, glycine or valine). US Patent 6,491,916 discloses that mutations in the region spanning about position 230 to about position 240 of a humanised antibody can produce particular advantages. Here it is explained that comparisons of antibodies that bind to Fc to those that do not bind to Fc suggest that changes in this region result in anti-CD3 antibodies that do not activate T cells. For example, some of the preferred antibodies comprise a mutation at position 234, at position 235, or at both. Anti-CD3 antibodies comprising one, two, three, four, five, or more mutations at one or more of positions 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, or 240, are expected to have advantages. This patent also discloses that an antibody having an IgGl Fc region and mutated to have alanine at both positions 234 and 235 does not bind to the CI q component of complement and start the complement-mediated cascade. Further, it is explained that the mutation Lys 320 to Gin has an affinity for Clq only slightly weaker than the wild type but is non lytic. US Patent 5,624,821 discloses that by changing any one of residues 318 (Glu), 320 (Lys) and 322 (Lys), to Ala, it is possible to abolish Clq binding. It points out that it is not necessary to replace the ionic residues only with Ala to abolish Clq binding, but that it will also be possible to use other alkyl-substituted non-ionic residues, such as Gly, He, Leu, or Val, or such aromatic non-polar residues as Phe, Tyr, Trp and Pro in place of any one of the three residues in order to abolish Clq binding. It will also be possible to use such polar non-ionic residues as Ser, Thr, Cys, and Met in place of residues 320 and 322. but not 318, in order to abolish Clq binding activity. US Patent 5,624,821 further discloses that replacing residue 297 (Asn) with Ala results in removal of lytic activity while only slightly reducing (about three fold weaker) affinity for Clq. It explains that it is thought this arises because the alteration destroys the glycosylation site and that the presence of carbohydrate is required for complement activition. It points out that any other substitution at this site will also destroy the glycosylation site. US Patent 5,624,821 also discloses that mutations on, adjacent or close sites in the hinge link region (e.g. replacing residues 234, 236 or 237 by Ala) indicate that alterations in residues 234, 235, 236 and 237 at least affect affinity for the Fc gamma Rl receptor. Of course one or more amino acid changes (typically conservative amino acid changes) may be incorporated that do not substantially affect biological properties. Possible mutations are therefore not restricted to those discussed above. Antibodies of whatever nature can be provided in monoclonal form (i.e. in combination with identical antibodies) or polyclonal form (i.e. in combination with different antibodies). Hybridomas capable of producing monoclonal antibodies of the present invention are also within the scope of the present invention. Antibody derivatives The term "antibody derivatives" is intended to allow for a wide range of structural changes that can be made relative to an antibody, provided that desired functional properties are retained. Thus, for example, binding affinity to TrkA is desirably retained. Preferably, the derivatives are also effective in one or more of the functional assays described herein. For the avoidance of doubt it is noted that all of the following are considered to be derivatives of an antibody of the present invention: a) a fragment of said antibody b) a multimer comprising a plurality of fragments of said antibody (referred to herein as a "fragment multimer") c) a fusion product of said antibody, fragment or fragment multimer and another moiety d) a variant of said antibody, fragment, fragment multimer, or fusion product, having at least 75% sequence identity therewith. Thus the term "derivative" is interpreted broadly. Turning now to fragments of the present invention, these are preferably at least seven amino acids long (Thus they are at least as long as the shortest CDR region shown in Figures la & lb for the heavy and light chains of the present invention). More preferably, they are at least ten, at least fifteen, or at least twenty amino acids long. They can be produced, by means of proteolytic digestion starting from intact antibodies or by inserting stop codons in the desired positions in vectors bearing the coding DNA sequences for the variable regions of the heavy and light chain. This can be done after the CH1 region to produce Fab fragments or after the hinge region to produce (Fab')2 fragments. Derivatives in the form of ScFv chains can be obtained by joining the variable regions of the heavy chain and of the light chain by means of a linker (Huston et al, PNAS, 85, 5879 (1988); Bird et al, Science, 242,423 (1988)). Fv or Fab fragments can be expressed in E. coli (Buchner and Rudolph, Bio/Technology, 9, 157 (1991); Skerra et al., Bio/Technology, 9, 273 (1991)) or also in eukaryotic cells, preferably mammal derived. Indeed a very range of fragment forms is possible, including those discussed by Holliger & Hudson in Nature Biotechnology, Vol 23, No 9, 1126-1136 (2005). These are all within the scope of the present invention. They can include fragments consisting of individual VH or VL chains (sometimes known as "domain antibodies" or "dAbs") or even fragments of said chains (e.g. individual CDR regions). Multimeric forms are also included, such as minibodies, bis(or higher)-ScFv, diabodies, triabodies, tetrabodies, Fab multimers, etc. (referred to herein as "fragment multimers"). Furthermore, various other moieties can be covalently linked with antibodies/fragments of the present invention so as to provide beneficial properties. Such "fusion products" are within the scope of derivatives of the present invention. The moiety may for example be a diagnostic agent, a therapeutic agent, a marking agent, an agent that increases the half life of the product, or an agent that reduces immunogenicity (preferably in a human host). For example, fusion products in the form of PEGylated antibodies/fragments may be provided. PEG has been predominantly used to reduce the immunogenicity and increase the circulating half-lives of antibodies. It may also have a beneficial effect on the use of antibodies in certain clinical settings such as tumour targeting. The parts of a fusion product can be linked together chemically. For example this may be done by cross-binding using heterobifunctional agents (e.g. SPDP, carbodiimide, glutaraldehyde, etc.). In the case of fusion proteins, these are preferably made using genetic engineering techniques. Thus appropriate coding sequences based on the genetic code can be provided encoding the desired fusion protein and can then be cloned into a host cell using a suitable expression vector. Expression may be under the control of a constitutive or inducible promoter. The expressed fusion protein can be purified using standard techniques (e.g. by using immunoffinity procedures). Cell-based or cell-free expression systems may be used. Fusion proteins may for example comprise antibodies/fragments of the present invention fused to cytotoxins. Resultant fusion proteins may then be used to target cells that express TrkA receptors, e.g. TrkA expressing tumour cells. The production of various cytotoxic immunotoxins is reported by Thorpe et al, Monoclonal Antibodies in Clinical Medicine, Academic Press, 168 (1982). Indeed a large number of cytotoxic agents are suitable for use in immunotoxins. Such agents include radionuclides such as iodine 131 or other isotopes of iodine, yttrium 90, rhenium 188 and bismuth 212 or other isotopes that emit alpha particles, a great number of chemotherapeutic drugs such as vindesin, methotrexate, adriamycin and cisplatin; cytotoxic proteins, such as proteins that inhibit ribosomes (e.g. pokeweed antiviral protein, Pseudomonas exotoxin A, diphtheria toxin, ricin A and clavin of vegetable origin), or agents active at the cell surface level (e.g. phospholipase enzymes such as Phospholipase C). Sometimes the cytotoxic region of the immunotoxin can be immunogenic and consequently limit the clinical usefulness of the fusion protein in case of chronic or long term therapy. An alternative to avoid the problem of the immunogenicity is to express in fusion with the binding domain of the antibody/derivative a protein able to interact with DNA and bind to this fusion protein the vector (e.g. plasmid) that contains the toxin expression cassette. The numerous positive charges of protamin, a human protein that binds DNA, can interact in stable fashion with the negative charges of the DNA, generating a fusion partner for the neutral charge antibody/derivative. This is much more stable and less immunogenic than the toxin itself. After internalization of the antibody-vector complex via receptor mediated endocytosis, the expression of the toxin causes the death of the cell. Moreover, if desired, inducible or cell-specific promoters can be provided in the toxin expression cassette. This approach is aimed at maximizing the selective elimination of tumour cells while minimizing toxicity side effects (Chen et al, Gene Ther., 2, 116 (1995)). Fusion proteins may also include fusions with other antibodies/derivatives. For example fusions of dAbs to specific antigens with other dAbs capable of binding long lasting serum proteins (e.g. serum albumin) have been used to increase serum half life. Variable heavy and light chain sequences of the present invention may form part of multivalent antibodies having specificity for one or more antigens, one of which is TrkA, or one or more epitopes within TrkA. Multivalent antibodies with specificity for one or more antigens, one of which is TrkA Expression systems Many expression systems can be used to provide antibodies/derivatives of the present invention. For example, prokaryotic systems can be used and are well characterized. E. coli is one of the prokaryotic hosts that is particularly useful for cloning the DNA sequences of the present invention. Moreover, a great number of well characterized promoters is available, e. g. from the lac or trp operon or β-lactamase or λ phage. Typically, these promoters control expression and bear binding site for the ribosome, for the correct start and finish of transcription and translation. It is possible to increase the half-life of the humanised immunoglobulins of the invention produced in prokaryotic systems by conjugation with polyethylene glycol (PEG). Other single-cell organisms, such as yeasts, can be used for expression. The host of choice is Saccharomyces, using suitable carriers provided with expression control, replication termination and origin sequences. Phage-display libraries bearing sequences of the variable regions of immunoglobulins have been well reported and can be used in binding studies [Cesareni, FEBS Letts, 307, 66 (1992); Swimmer et al. PNAS, 89, 3756 (1992); Gram et al. PNAS, 89, 3576 (1992); Clackson et al. Nature, 352, 624 (1991); Scott & Smith, Science, 249, 386 (1990); Garrard et al. Bio/Techniques, 9,1373 (1991)]. Insect cell cultures can also be used, typically utilising cells of S2 Drosophila transfected in stable fashion or cells of Spodoptera frugiperda with the expression system based on the Baculovirus (Putlitz et al. Bio/Technology, 8, 651 (1990)). Plants and cultures of vegetable ceils can even be used (Larrick & Fry, Hum. Antibodies Hybridomas,2, 172 (1991); Benvenuto et al. Plant Mol. Biol, 17 865 (1991); Durin et al. Plant Mol. Biol,; 15,281 (1990); Hiatt et al. Nature, 342,76 (1989)) It is also possible to use tissue cultures of mammal cells to express the polypeptides of the present invention. This can be advantageous in obtaining human glycosylation patterns. Different isotypes can be expressed. IgGl has proven to be the most effective isotype in the induction of the immune response (Winnacker, From Genes to Clones, VCH Publishers, NY, (1987)) whilst IgG4 is often used for diagnostic applications (Riechmann et al., Nature, 332,323 (1988)). Mutated forms abolishing/reducing activation of complement may also be provided, as discussed earlier with reference to US Patent No 6,194s551., US Patent 5,624,821 and/or US Patent 6,491,916. In particular, mammalian cells are preferred. A great number of host cell lines have been developed for the secretion of intact immunoglobulins, among them are CHO cell lines, several COS cell lines, the HeLa cells, myeloma cell lines (NSO, SP/2, YB/0 e P3X63.Ag8.653), transformed B cells or hybridomas. Expression vectors for these cells can include expression control sequences, such as a replication origin, a promoter, an enhancer (Queen et al, PNAS, 86:10029 (1989)), and the sequences required for ribosome binding, RNA splicing and polyadenylation, and sequences for transcription termination. The expression control sequences of choice are promoters derived from immunoglobulin genes and from viruses, such as SV40, Adenovirus, Bovine Papilloma Virus, Cytomegalovirus and the like. Generally, the expression vector includes a selectable marker, such as the resistance to neomycin. For the expression of humanised antibodies, it is preferable to cultivate the mammal cell lines with a serum-free medium. For example, the HUDREG-55 cell line can easily be grown in Serum-Free and Protein-Free Hybridoma Medium Cat. No. S-2897 from Sigma (St. Louis, Mo.). Nucleic Acids, Vectors, Transgenic animals Nucleic acid sequences encoding the antibodies/derivatives/antibody chains of the present invention can be produced by standard techniques, given that the amino acid sequences for the key variable regions are provided herein and that corresponding coding sequences can be provided using the genetic code. These sequences can be incorporated into expression vectors and/or cloned into cells. Indeed techniques for producing and cloning "reshaped antibodies" with rodent CDR regions and humanised framework regions are now well known. They are discussed for example in Jones, Dear, Foote, Neuberger and Winter, Nature, 321, 522-4 (1986); in Riechmann, Clark, Waldman and Winter, Nature, 332, 323-327 (1988) and in Verhoeyen, Milstein and Winter, Science, 239, 1534-1536 (1988). Such nucleic acids can be incorporated into expression vectors, including plasmids, phage, etc., as is well known in the art and is discussed above. Nucleic acids of the present invention can also be used to design probes or primers. These can be used for example to isolate or amplify nucleic acids of the present invention. Probes or primers are therefore within the scope of the present invention. Typically they are at least 10, at least 15 or at least 20 bases long. Preferably they hybridise under stringent conditions to nucleic acid strands that encode antibodies/derivatives of the present invention or to complementary strands thereof. One example of stringent hybridisation conditions involves using a pre-washing solution of 5 X SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0) and attempting hybridisation overnight at 55°C using 5 X SSC. However, there are many other possibilities. Some of these are listed in Table I of W098/45435, for example (See especially the conditions set out under A-F of that table and, less preferably, those listed under G to L or M to R). In a further aspect of the present invention, the nucleic acids can advantageously be used to provide transgenes for use in producing non-human transgenic animals, preferably mice. Here the antibody/derivative may be expressed in an inducible way, or under the control of constitutive promoters. Such animals can be advantageously used to study and test drugs for human pathologies wherein the NGF/TrkA interaction is inhibited and, particularly, neurodegenerative pathologies. The antibody/derivative can be advantageously expressed in a retrievable body fluid such as milk or serum, from which itcan be retrieved and purified using standard techniques. Transgenes used to produce the transgenic animals may comprise the relevant coding sequence(s) operatively bound to a promoter, usually in combination with an enhancer sequence, such as that of the rodent immunoglobulin or the promoter/enhancer of the casein gene (Buhler et al., Bio/Technology; 8.140 (1990); Meade et al, Bio/Technology, 8,443(1990)). The transgenes can be transferred into the cells or embryos by means of homologous recombination. A wide range of non-human transgenic animals can be produced, including mice, rats, sheep, cows, goats, etc. (see WO 91/08216). It will be appreciated from the foregoing description that the present invention provides a range of new antibodies, derivatives, nucleic acids, etc. If desired, these can be provided in substantially purified form. For the purposes of the present invention this means that they are the majority of the dry weight of a particular composition. For example they may represent at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 98% of said dry weight. They may be provided in isolated form. This means that they are removed from one or more other components with which they may be normally associated in nature. (For example a nucleic acid may be provided in a form that is isolated from a cell). They may be provided in a variety of other forms. For example they may be fused to heterologous moieties and/or they may be immobilised. All of the above forms are within the scope of the present invention. The present invention will now be described by way of example only, with reference to the accompanying drawings, wherein: Figures la & lb show amino acid sequence alignments for various heavy and light chains. Figure 2 shows the results for antigen binding specificity towards TrkA-IgG in respect of supernatants from various clones resulting from an experiment involving transient expression of the humanised MNAC13 variants in COS~7 cells. Figure 3 the results of an experiment in which cellular binding of the antibodies to TrkA expressed on TF-1 cells was analysed by cytofluorimetric analysis. Figure 4 shows the results of a further analysis in which the best binders identified from Figure 3 (BXhVH3VL3, BXhVHSVLl, BXhVH5VL3, and HuMNACWOv) were compared to HuMNACWO. Figure 5 shows the results of an assay in which different humanised candidates were assayed in parallel with murine MNAC13 antibody (muMNACEP), chimMNAC13, HuMNACWO, and Human IgGl as standard control. Figure 6 shows the heavy and light chains for BXhVHSVLl, including the constant regions (the first amino acid of the constant region is underlined.) Figure 7 shows the heavy chain for BXhVHSVLl N297A, including the constant region (the first amino acid of the constant region is underlined and the 297 A position is bold and underlined). Figure 8 shows BXWH5VL1 and BXWH5VL1 N297A binding to cell lines expressing huTrkA. Figure 9 shows the effect of various antibodies on NGF-induced MIP-1β production in human mast cell line HMC-1. Figure 10 shows binding of BXhVH5VLl to cell bound Fc receptors on THP1 cell line compared to BXhVH5VLl N297A. Figure 11 shows an experiment demonstrating the analgesic effect of local intradermic injection of BXhVH5VLl N297A or control hlgG when co-injected with rhNGF Figure 12 shows an experiment demonstrating the analgesic effect of local intradermic injection of muMNACEP or control mlgG when co-injected with rhNGF Figure 13 shows an experiment demonstrating the analgesic effect of systemic administration of BXhVHSVLl N297A when compared to control hlgG in an animal model of NGF-induced pain. Figure 14 shows an experiment demonstrating the analgesic effect of systemic administration of muMNACEP when compared to control hlgG in an animal model of NGF-induced pain. Examples Before discussing the examples in detail, some of the nomenclature used therein is set out below: muMNACEP This term is used to indicate the murine antibody MNAC13, as disclosed in EPI181318 The heavy chain variable region of this antibody is referred to herein as mVHEP (SEQ ID NO. 15V The light chain variable region is referred to herein as mVLEP (SEQ ID NO. 16). HuMNACWQ This term is used to indicate the humanised antibody MNAC13 disclosed in WO 05/061540 The heavy chain variable region of this antibody is referred to herein as HuVHWO (SEQ ID NO. 17). The light chain variable region is referred to herein as HuVLWO rSEQIDNO. 18) HuMNACWOv This terms is used to indicate a variant of the antibody disclosed in HuMNACWO (see above) in which the heavy chain CDR3 region has been replaced with a CDR3 region corresponding to that present in muMNACEP. The variant is novel and is within the scope of the present invention. The heavy chain variable region of this antibody is referred to herein as HuVHWOv(SEQ ID NO. 6). The light chain variable region can be referred to herein as HuVLWOv. However, in order to avoid duplication, it is not shown in Figure 1 b, because it is the same as HuVLWO (SEP ID NO. 18). ChimMNAC13 This corresponds to muMNACEP, but has human constant regions instead of mouse constant regions. The heavy chain variable region of this antibody is referred to herein as mVHEP (SEQ ID NO. 15) The light chain is referred to herein as mVLEP (SEQ ID NO. 16) 3-23*01 (SEP ID NO. 19). JH4 (SEP ID NO. 20). L6*01 (SEO ID NO. 21) and JK1 (SEQIDNO. 22) These are coding sequences derived from human germline genes. They are used for assessing degrees of humanisation in Table 1. Thus if there are no changes relative to a human germline sequence it is considered that there is 100% humanisation. [The percentage humanisation = number of changes X 100] total number of residues compared The table below shows the percentage humanisation for' the different variants: Table 1 Sequence variant Number of % Number of % humanisation murine AA in Humanisation murine AA (related to the FW/number (related to FW including CDR complete of total AAin the FW sequence) AA/number of total AA in the variable region variable sequence) BXhVHl 0/87 100 36/123 70.7 BXWH2 3/87 96.6 39/123 68.3 BXhVH3 3/87 96.6 39/123 68.3 BXhVH4 3/87 96.6 39/123 68.3 BXhVH5 5/87 94.2 41/123 66.7 BXhVHWO 12/87 86.2 48/123 61.0 BXhVLl 0/80 100 26/106 75.5 BXhVL2 4/80 95 30/106 71.7 BXhVL3 6/80 92.5 32/106 69.8 BXhVL4 6/80 92.5 32/106 69.8 BXhVLS 6/80 92.5 32/106 69.8 BXhVL6 8/80 90 34/106 67.9 BXhVL7 8/80 90 34/106 67.9 BXhVL8 11/80 86.2 37/106 65.1 BXhVLWO 9/80 88.8 35/106 67 It can be seen that all of the variant variable chains have a degree of humanisation over the framework regions of over 85%. "BX" sequences The sequences labelled with a code beginning with "BX" are novel sequences of the present invention. The letters following "BX" are either VH or VL to indicate a heavy or light variable chain respectively. The sequences are then simply numbered consecutively in the order in which they are shown in Figures la & lb for a given chain. There are five heavy chain sequences. Thus they are numbered: BXhVHI (SEP ID NO. n BXhVH2 (SEP ID NO. 2) BXhVH3 (SEQ ID NO. 3) BXhVH4 (SEQ ID NO. 4) BXhVH5 (SEP ID NO. 5) There are eight light chain sequences. Thus they are numbered: BXhVLl (SEP ID NO. 7) BXhVL2 (SEP ID NO. 8) BXhVL3 fSEQ ID NO. 9) BXhVL4 (SEOIDNO. 10) BXhVL5 (SEOIDNO. 11) BXhVL6 (SEOIDNO. 12) BXhVL7 (SEOIDNO. 13) BXhVL8 (SEOIDNO. 14) The chains can be combined in antibodies or derivatives thereof. The forty possible combinations have all been produced and are: BXhVHIVLl, BXhVHlVL2, BXhVHlVL3, BXhVHlVL4, BXhVHlVL5, BXhVHlVL6, BXhVHlVL7, BXhVHlVL8, BXhVH2VLl, BXhVH2VL2, BXhVH2VL3, BXhVH2VL4, BXhVH2VL5, BXhVH2VL6, BXhVH2VL7, BXhVH2VL8, BXhVH3VLl, BXhVH3VL2, BXhVH3VL3, BXhVH3VL4, BXhVH3VL5, BXhVH3VL6, BXhVH3VL7, BXhVH3VL8, BXhVH4VLl, BXhVH4VL2, BXhVH4VL3, BXhVH4VL4, BXhVH4VL5, BXhVH4VL6, BXhVH4VL7, BXhVH4VL8, BXhVH5VLl, BXhVH5VL2, BXhVH5VL3, BXhVH5VL4, BXhVH5VL5, BXhVH5VL6, BXhVH5VL7, BXhVH5VL8. "N297A" The designation "N297A" after the name of an antibody indicated that position 297 of the heavy chain constant region is mutated from N to A. The sequence of BXhVH5VLl N297A is provided as SEQ ID No. 23. Expression vectors The appropriate coding sequences were fused to a sequence coding for a secretory signal 5' and a splice donor sequence 3' to the cDNA for cloning into an antibody expression system. The DNA fragments were cloned into IgGl expression vectors. These expression vectors were based on genomic sequences encoding the human constant domains and cloning cassettes for the insertion of the selected cDNA fragments of the hVH and hVL sequences. Transient expression of the humanised MNAC13 variants in COS-7 cells and determination of antibody titers Each combination of Heavy and Light chain was transiently transfected in COS-7 cells and antibody titer was determined. The expression vectors coding for the light chain and for the heavy chain were transiently cotransfected into COS-7 cells by lipofection using Lipofectamin according to the manufacturer's instructions (Invitrogen, Germany) in a 24-well format. After transfection the medium was replaced by DMEM containing 10%FCS and 2% L-glutamine and the supernatants of the COS-7 cells were collected 4 days after transfection. The antibody titer of the humanised antibodies secreted into the supernatants of transfected COS-7 cells was analyzed by a sandwich ELISA. Briefly, a mouse anti-human kappa chain recognizing antibody (BD) was immobilized on a 96 well plate, blocked and incubated with diluted supernatant of transfected COS-7 cells. The presence of antibodies was detected by a POD conjugated rabbit anti-human IgG (H+L) antibody (Dianova, Germany). A chimeric control antibody was used as a standard in concentrations from 1 to 10 ng/ml. The determined antibody concentrations were further adjusted by an internal standard sample having a standardized antibody concentration. Example 1 Comparison of humanised antibody binding towards TrkA-IgG in ELISA Based on the determined antibody concentration, supernatants of all samples were adjusted to the same antibody concentration. The binding activities of all humanised antibody variants were analyzed by a TrkA-IgG antigen ELISA. They were compared to the binding activities of the ChimMNAC13 and HuMNACWOv. Antibodies and antigens were thawed, aliquoted and stored at - 20°C. Aliquots of the antibodies in use were stored at 4 °C for a maximum of two weeks. Antigen ELISA was performed as follows: Maxisorb plates (Nunc, Germany) were coated with 0.125, 0.25, 0.5, and 1µ,g/ml TrkA-IgG. To check the specificity of antibody-antigen binding TrkB-IgG (lµ.g/ml) as a negative control was used. Transiently expressed antibody variants were used at 1, 10, and 100 ng/ml. Detailed procedure as follows: Coating Plates: Nunc MaxiSorp 96well lOOµlAvell of TrkA-IgG at 2 ng/ml in Carbonate Buffer 0.1M pH 9.6 (TrkB-IgG used as negative control) Seal plate and incubate overnight at +4°C Wash 3 times with 200 µ.1 of wash buffer Blocking Block plates by adding 200µl of SuperBlock Blocking Buffer in PBS. (Pierce Prod # 37515) to each well. Immediately empty the plate by inversion. Repeat two additional times. Incubate at 37°C for 2 hours. Primary Antibody Discard supernatant and add 100µ1 of purified mAb appropriately diluted in TEST Buffer (standard curve range: 50-5000 pg/ml). Seal plate and incubate at 37°C for 2 hours. (In order to increase sensitivity incubate overnight at +4°C) Wash 4 times with wash buffer. Secondary Antibody Add 100µl of HRP-conjugated Goat anti mouse IgG (Pierce cat. 31430) diluted 1:10000 in TEST Buffer. Incubate at 37°C for 1 hour. Wash 4 times with wash buffer. Development Add lOOµl of Substrate solution to each well. Incubate at room temperature. Stop the reaction with lOOµl of H2S04 2M. Determine the optical density of each well using a microtiter reader at 450 run. Results The results for supematants from the various clones evaluated for antigen binding specificity by using the ELISA assay are shown in Figure 2. Briefly, the specific antigen TrkA-IgG (black bars) and the negative control TRKB-IgG (white bars) were coated at 1 µg/ml concentration on different 96-well plates. Antibodies supematants were quantified, appropriately diluted, and tested at 5 ng/ml concentration. After washing, binding was detected with the appropriated HRP-labeled secondary antibody, revealed by a chromogenic reaction and quantified by OD450/630 nm measure. The majority of the humanised antibodies show a comparable selective affinity for high density TrkA antigen. In addition, their binding specificity is not significantly different from parental murine anti-human TrkA antibody and its chimeric isoform, indicating that antigen seiectivity has been fully preserved along the humanisation procedure. Example 2 Cellular binding assay of new candidates by cytofluorimetric analysis of TrkA surface expression on TF-1 cells Procedure Harvest cells from culture, preparing a single cell suspension. (In order to obtain maximum antigen expression split cells 1:3 the day before). Distribute 0.3-0.4 x 106cells/sample and wash IX with cold FACS buffer (PBS pH 7.4 + 0.1% NaN3 + 0.1% BSA). Centrifuge at 350 x g for 5 min. Discard supernatant and Keep tubes on ice. Fc Receptors Blocking Add 50 µI/sample of Human IgG [300 ug/ml] in FACS buffer and mix by gently vortexing. Incubate at 4°C for 15 min. Primary Antibody Add 100µl/sample of Primary Antibody muMNAC13 [4 ug/ml] in FACS buffer and mix by gently vortexing. As negative control use purified mouse IgGl isotype control at the same concentration. Incubate at 4°C for 30 min. Wash 2X with 1ml of FACS buffer, spin 5 min at 350 x g, and discard the supernatant. Secondary Antibody Add 100 µl/sample of Donkey anti Mouse IgG (H+L) R-Phycoerythrin conjugated Jackson ImmunoResearcb cat.# 715-116-151 in FACS buffer and mix by gently vortexing. Incubate at 4°C for 30 min. Wash 2X with lral of FACS buffer, spin 5 min at 350 x g, and discard the supernatant. Re-suspend in 0.5 ml of FACS Buffer. Acquire sample data on flow cytometer. Results TF-I cells were stained with supernatants from all the clones as well as HuMNACWO and HuMNACWOv antibodies as controls (4 µ.g/ml) for 30 minutes at 4°C. Staining was revealed by an appropriate PE-labeled secondary antibody and quantified by cytofluorimetric analysis to evaluate the fluorescence intensity of the binding. The results are shown in Figure 3, which is based upon the table below. Table 2 Geo Mean Fluorescence Fold Increase N°. Variants Mean ± S.D. Mean ± S.D. 1 mVHEP/mVLEP 11.0 ± 2.2 3.2 ± 0.7 2 hVHWOv/hVLWO 8.9 ± 1.9 2.6 ± 0.6 3 hVHl/hVLl 5.7 ± 0.4 1.7 ± 0.1 4 hVHl/hVL2 4.6 ± 0.4 1.3 ± 0.1 Geo Mean Fluorescence Fold Increase 5 hVHl/hVL3 6.1 ± 0.6 1.8 ± 0.2 6 hVHl/hVL4 5.1 ± 0.5 1.5 ± 0.2 7 hVHl/hVL5 4.5 ± 0.3 1.3 ± 0.1 8 hVHl/hVL6 4.9 ± 0.4 1.4 ± 0.1 9 hVHl/hVL7 5.1 ± 0.4 1.5 ± 0.1 10 hVHl/hVL8 5.2 ± 0.1 1.5 ± 0.0 11 hVH2/hVLl 9.2 ± 1.3 2.6 ± 0.4 12 hVH2/hVL2 6.4 ± 0.7 1.8 ± 0.2 13 hVH2/hVL3 10.8 ± 3.3 3.1 ± 0.4 14 hVH2/hVL4 6.1 • 0.3 1.8 ± 0.1 15 hVH2/hVL5 6.4 ± 0.2 1.8 ± 0.1 16 hVH2/hVL6 6.4 ± 0.7 1.8 ± 0.2 17 hVH2/hVL7 6.5 ± 0.8 1.9 ± 0.3 18 hVH2/hVL8 6.5 ± 1.0 1.9 ± 0.3 19 hVH3/hVLl 8.6 ± 1.5 2.5 ± 0.5 20 hVH3/hVL2 7.1 ± 2.1 2.0 ± 0.6 21 hVH3/hVL3 12.6 ± 0.6 3.6 ± 0.2 22 hVH3/hVL4 7.1 ± 0.1 2.0 ± 0.0 23 hVH3/hVL5 6.9 ± 0.5 2.0 ± 0.2 24 hVH3/hVL6 6.4 ± 0.5 1.8 ± 0.2 25 hVH3/hVL7 7.1 ± 1.0 2.0 ± 0.3 26 hVH3/hVL8 6.5 ± 1.2 1.9 ± 0.3 27 hVH4/hVLl 10.4 ± 2.4 3.0 ± 0.7 28 hVH4/hVL2 8.3 ± 2.5 2.4 ± 0.7 29 hVH4/hVL3 10.9 ± 3.0 3.1 ± 0.9 30 hVH4/hVL4 8.0 ± 2.2 2.3 ± 0.6 Geo Mean Fluorescence Fold Ii icrease 31 hVH4/hVL5 8.6 ± 1.7 2.5 ± 0.5 32 hVH4/hVL6 8.0 ± 1.4 2.3 ± 0.4 33 hVH4/hVL7 8.7 ± 2.5 2.5 ± 0.7 34 hVH4/hVL8 8.3 ± 1.9 2.4 ± 0.6 35 hVH5/hVLl 11.3 ± 2.7 3.2 ± 0.8 36 hVH5/hVL2 8.6 ± 2.3 2.5 ± 0.7 37 hVH5/hVL3 13.7 ± 2.1 3.9 ± 0.6 38 hVH5/hVL4 9.1 ± 2.2 2.6 ± 0.7 39 hVH5/hV.L5 8.3 ± 2.0 2.4 ± 0.6 40 hVH5/hVL6 9.1 ± 1.5 2.6 ± 0.5 41 hVH5/hVL7 8.6 ± 2.0 2.5 ± 0.6 42 hVH5/hVL8 8.3 ± 1.6 2.4 ± 0.5 43 hulgG 3.5 ± 0.0 1.0 ± 0.0 The results showed that all the clones tested as well as HuMNACWOv positively detected the membrane-associated TrkA receptors on TF1 cells though to a different extent. HuMNACWO is not able to stain TF1 cells, which have a low density of surface TrkA receptors. To further confirm, out of 40 clones tested, the best binders were selected to be further analyzed. As evaluated in two separate experiments (Figure 4) BXHVH3VL3, BXhVH5VLl, BXhVH5VL3, and HuMNACWOv were compared with HuMNACWO. The selected leads were confirmed good binders and slightly better performers when compared to HuMNACWOv. The humanized antibody isoforms BXhVH5VLl N297A and BXhVH5VLl together with the reference antibodies muMNACEP and HuMNACWO were also assayed for binding capability on TF-1, HMC-1 and PC12-hTrkA cell lines which express different levels of surface receptor hTrkA. As shown in Figure 8, BXhVH5VLl N297A and BXhVH5VLl antibodies comparably bind all the tested cell lines, independently of the receptor density on the cellular surface. Both antibodies appear to bind more efficiently when compared to the parental muMNACEP. HuMNACWO only binds high surface receptor density cell linePC12-hTrkA. Example 3 Comparison of humanised antibody biological activity in vitro with a proliferation assay on TF1 cells To measure the ability of anti-human TrkA monoclonal antibodies to block cell surface TrkA- p-NGF mediated biological activity, a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al,, 1989, J. Cell Physiol. 140:323-334) was used. TF-1 cells were incubated with various concentrations of the antibodies for 0.5 hour at 37° C in a 96 flat well culture plate. Following this pre-incubation period, recombinant human p-NGF (rec-hu-β-NGF, R&D Systems) was added to the cell-antibody mixture. The assay mixture in a total volume of 200 µL, containing antibody at different concentrations indicated, human β-NGF at 5.0 ng/mL and TF-1 cells at 5 x 103 cells/well, was incubated at 37°C for 5 days in a humidified C02 incubator. After that period the plates were centrifuged and after removal of the supernatant, frozen at - 80°C in order to lyse the cells. CyQUANT Cell Proliferation Assay Kit (Molecular Probes) was used for measuring cell proliferation according to the manufacturer's instructions. This experiment was performed twice. Results Different humanised candidates were assayed in parallel with murine MNAC13 antibody (muMNACEP), chimMNACB, HuMNACWO and Human IgGl as standard control (Figure 5). The IC50 were calculated for each curve and the results are given in the table overleaf. It was found that antibody BXhVH5VLl was the best performer among the candidates. The heavy and light chains for this antibody are therefore shown in Figure 6. The average IC50 for murine MNAC13 over a series of experiments was.0.54 ± 0.47 µg/ml. Table 3 Proliferation assay on TF1 cells IC50 (µg/ml) Mean SD muMNACEP 0.54 0.47 EXP-1EXP-2 0.06 0,58 ChimMNACB BXhVH5VLl 0.17 1.84 BXhVH3VL3 0.41 2.38 BXhVH5VL3 1.40 1.21 HuMNACWO HulgGstd Example 4 Surface plasmon resonance analysis Surface plasmon resonance analysis was used to measure the association and dissociation rate constants for binding kinetics of the different antibodies (murine, chimeric, 5 humanised variants) towards TrkA-IgG using BIACORE 2000 (Biacore AB, Uppsala, Sweden). TrkA-IgG was immobilised on a CM-5 sensor chip according to manufacturer's conditions, in a way to achieve an immobilization density of 1100 RU. Each antibody sample was analyzed in antibody concentration ranges of 20-0.63 µg/ml. Calculations from the sensograms were performed by using the BIA evaluation version 3 (1999) software. Analysis of the individual sets of sensograms was performed with the BIA evaluation version 3 (1999) software. Among the different models tested to fit the kinetics data, the best fitting was obtained with the "separated 1:1" algorithm. In this model, only a defined range of the early association and dissociation curves was used for the calculation. It is assumed that during these early phases of the curve, the overlay effects like mass transfer, rebinding or others do not affect the calculations. Results The dissociation constant (KD) was determined for various antibodies and is set out in the table overleaf in the order in order of increasing value. The KD value has molar units (M), which correspond to the concentration of ligand at which a binding site on a particular protein is half occupied. The smaller the value, the more tightly bound the ligand, or the higher the affinity between ligand and protein (here between antigen and antibody). Table 4 Antibody Kon (1/Ms) Koff (1/s) KD [M] ChimMNAC13 2.68x105 3.53x10-4 1.51x10-10 MNAC13 8.50x105 1.67x10-4 2.50xl0-10 BXhVH5 VL1 7.68x105 4.70x10-4 6.15xlO-10 BXhVH5VL3 l.OOxlO6 6.38X10-4 6.62xl0'-10 BXhVH3VL3 3.25x10' 4.42x10-4 1.45xl0-9 HuMNACWOv 1.62xl06 3.86x10-3 2.48x10-9 HuMNACWO (separate experiment) 7.39x10' 3.09x10-2 4.18xl0-9 It can be seen from the above table the calculated KD for the murine and the chimeric isoforms are very comparable with one another. They are slightly lower but of the same order of magnitude as the humanised variants BXhVH5VLl and BXhVH5VL3. On the contrary, the humanised variants HuMNACWOv and BXhVH3VL3 display a one order of magnitude higher KD than that observed for the murine and the chimeric variants. However, the KD values here are still lower than for the prior art humanised antibody HuMNACWO. Indeed, preferred KD values for antibodies/variants of the present invention using this model are below 4,18 x 10-8 (Thus they are lower than for the value for the humanised prior art antibody HuMNACWO). More preferably they are below 2.4 8 x 10~9 (thus they are lower than the HuMNACWOv, which is a variant of HuMNACWO with the same framework regions, but with changes in the third CDR of the heavy chain). Most preferably the KD values are below 1 x 10"9 (thus they are of the same general order of magnitude as for the murine and the chimeric isoforms). Consistently, the ranking given in the above table, which is based on the calculated data using the "separated" algorithm, reflected very well the ranking obtained by visual inspections of the sensograms of all investigated variants in overlay plots. Example 5 Comparison of humanised antibody biological activity in vitro with a chemokine secretion assay on HMC-1 mast cell line NGF acts as an important intermediate in inflammatory pain, contributing to both peripheral and central sensitization. The sensitization of peripheral nociceptors can be very rapid and can involve non-neural cells such as mast cells. To measure the ability of anti-human TrkA monoclonal antibodies to inhibit (3-NGF-induced MlPla secretion, a biological assay using the mast cell line, HMC-1 (Ahamed, J. et al J Immunol. 2004 Jun 1;172(11):6961-8.) was used. HMC-1 cells (0.1 xl06/well) were plated in triplicate in complete growth medium in a 96 flat well culture plate and incubated with various concentrations of monoclonal antibody for 0.5 hour at 37° C. Following this pre-incubation period, recombinant human p-NGF (rec-hu-p-NGF, R&D Systems) was added to the cell-antibody mixture to a final concentration of 50 ng/ml and incubation at 37° C was extended for 6 hours in a humidified C02 incubator. Supernatants were then harvested and levels of MlP-lp were quantified by sandwich ELISA using a DuoSet® Elisa Kit for Human CCL4/MIPl-fi from R&D System (Cat. Nr. DY271). Data obtained were expressed as % of response and analyzed with GraphPad Prism 5 software using a nonlinear regression analysis, log(inhibitor) vs. normalized response—variable slope equation. Results BXhVH5VLl N297A antibody was assayed in parallel with murine muMNACEP, HuMNACWO, and Human IgGl as standard control . The IC50 values were calculated for each curve and the results are shown in Figure 9. The inhibitory activity of BXhVHSVLl N297A was significantly higher than that of the humanised antibody HuMNACWO. Example 6 BXhVH5VLl N297A and BXhVHSVLl in vitro characterization. Evaluation of binding to cellular FcRs on THP-1 cells Human acute monocytic leukemia cell line THP1 (ATCC) was cultured in RPMI1640/GLUTAMAX (Invitrogen) + 10% Foetal Bovine Serum (Invitrogen) + Pen/Strep, and maintained between 2-9x100,000 cells/ml. Cells were harvested from culture and prepared as a single cell suspension. 0.3-0.4 x 106cells/sample were then distributed in 96-well round-bottom tissue culture plates (Costar, Cambridge, MA) and washed IX with cold FACS buffer. After centrifugation at 350 x g for 5 min., supernatant is discarded and plates put on ice. Binding of IgG to FcyRs on THP-1 cells was performed by incubating monomeric IgGs in FACS Buffer starting from 30 µg/ml to 0.02 µg/ml (dilutions 1:3) in a total volume of lOOµl at 4 °C for 30 min. Cells were then washed three times with 200µl of FACS buffer, and IgG binding detection is acheived by adding 100 \xl of Donkey anti Human IgG (H+L) R-Phycoerythrin conjugated (Jackson Immuno Research cat.# 709-116-149) 1:100 in FACS buffer. After gentle vortexing. cells were incubated at 4°C for 30 min. Plates were washed 2X with 200µ.l of FACS buffer, cells are finally resuspended and transferred in 0.5 ml of FACS Buffer and acquired by using a flow cytometer. Results Figure 10 clearly shows that as expected based on prior art disclosures (see US Patent 5,624,821 Winter) the mutated isoform BXhVH5VLl N297A is devoid of significant binding capability to cellular Fc receptors. Example 7 In vivo Experiments In vivo experiments that were performed to further assay the antibodies/derivatives of the present invention are set out below; NGF-mediated pain models Nerve growth factor (NGF) and its receptor TrkA are crucial mediators of the pain sensations characteristic of inflammatory pain. Classically, NGF is known as a developmental survival factor for sensory and sympathetic neurons but it continues to be synthesized in adult animals in the periphery, where it is transported retrogradely to the cell bodies of sensory neurons (Hendry et a!., 1974, Otten et al 1980). Inflammation and nerve injury cause the release of NGF which stimulates primary afferent fibres and induces behavioural sensitisation. Subcutaneous chronic treatment with NGF in rats causes hyperalgesia and alters local cutaneous sensation (Lewin et al., 1993; Andreev et al., 1995). Intradermal injection of rhNGF into human forearm and masseter muscle in humans causes hyperalgesia, allodynia and alters local cutaneous sensation that began 3hrs following injection and peaked 1-7 days post injection and recovered by day 21 (Dyck et al., 1997; Svensson et al., 2003). Thus, injections of rhNGF into the rat hindpaw were used here as a model of behavioural sensitisation that was specifically generated by NGF. The present experiments involved two different protocols: 1. We first examined whether intradermal injection of recombinant human (rh) NGF alone in rat paw could cause behavioural sensitisation as measured by standard nociceptive tests for hyperalgesia (Hargreave's plantar test). We then established whether intradermal co-injection of the murine IgG, muMNACEP, human IgG, and BXhVHSVLl N297A_antibodies at a dose of 100u.g could affect the rhNGF-induced sensitisation. Murine IgG1 and human IgG antibodies ware used as a negative controls at the appropriated dosages. 2. We then established whether systemic pre-treatment of muMNACEP antibody (at doses of 8 and 1 mg/Kg, i.p.) and BXhVH5VLl N297A (doses of 8, 3, and 1 mg/Kg, i.p.) could affect the peripherally induced rhNGF sensitisation. In the first protocol, whereby treatments where administered locally, male Lewis rats (Charles River, 5-6weeks 200g) were used with 8-9 animals per group and 4 experimental groups, injected according to the set method. Injections were carried out blind. A summary of the treatments is outlined in the table below. muMNACEP /BXhVH5VLl N297A mIgG,/hIgG Intradermal 100µg + 500ng 100µg + 500ng treatments rhNGF, n=9 rhNGF, n-9 In the second protocol, whereby treatments were administered systemically (i.p.) 24hrs prior to rhNGF paw injection, male Lewis rats (Charles River, 5-6weeks 200 g) were used with 10-12 animals per group and 10 experimental groups. Injections were carried out blind. A summary of the treatments is outlined in the table below. muMNACEP mlgG, BXhVH5VLl N297A hlgG Systemic i.p. lmg/Kgn=10 lmg/Kg lmg/Kg n= 10 lmg/Kg 24hr pre-treatments 8mg/Kgn= 10 n=12 8mg/Kg n= 8 3mg/Kgn=10 8mg/Kg n= 12 n=10 3mg/Kg n=I0 8mg/Kg n= 12 rhNGF 500ng 500ng 500ng 500ng intradermal Assay All animals were numbered and then habituated to behaviour- testing procedures 24-48hr prior to commencement of the experiment. Behaviour readouts were the paw withdrawal latency to the plantar test as a measure of hyperalgesia. Baseline recordings were taken to establish paw withdrawal latencies. Nociceptive sensitivity was induced by intradermal rhNGF injected at Time point 0 and behavioural nociceptive sensitivity was monitored 30 minutes, 1 hour, 2 hours, 24 hours and 48 hours following rhNGF injection. Treatments were administered blind as follows: Protocol 1: Treatment administration by intradermal injection at Time 0. Protocol 2: Treatment administration by systemic single injection IP 24h before rhNGF paw injection. Baseline plantar and von Frey tests were performed before drug treatments were administered. Hyperalgesia measurements were taken 30min, lhr, 2hrs, 24hrs and 48hrs after rhNGF injection. Three to four recordings were taken for each hindpaw ipsilateral (right paw, rhNGF-injected) and contralateral (left paw, un-injected) to rhNGF injection. Data from animals from individual treatment groups were collated, and means and standard deviations were calculated for the controlateral and ipsilateral paw responses. The presence of hyperalgesia was indicated by a significant reduction in the paw withdrawal latency (recorded in seconds) in the rhNGF-injected ipsilateral hindpaw when compared with the control contralateral paw (by paired t-test) and when compared with the pre-injection/pre-treatment baseline (by one-way ANOVA). Intradermal anti-hyperalgesic efficacy We compared the anti-hyperalgesic efficacy of BXhVH5VLl N297A (Figure 11) and muMNACEP (Figure 12) (plus mlgG1 and hlgG as relative controls) by intradermal injection in this model of NGF-induced hyperalgesia. When BXhVH5VLl N297A and muMNACEP were co-injected with rhNGF, there was no significant development of hyperalgesia, as indicated by no significant difference between ipsilateral and contralateral paw withdrawal responses (Figures 11 and 12). Hyperalgesia in ipsilateral paw responses were always present following co-injection with the negative controls (mlgG1 hlgG). Data are represented as means ± 95% CI, before (baseline) and following intradermal injection of 500ng rhNGF with respective treatments (at arrow). Significant reduction in paw withdrawal latency ipsilateral is indicated by '*' '**' (p Systemic anti-hyperalgesic efficacy We compared the anti-hyperalgesic efficacy of BXhVH5VLl N297A, muMNACEP mlgGl, and hlgG by systemic injection in this model of NGF-induced hyperalgesia. Three different doses of BXhVH5VLl N297A and control hlgG (1, 3, and 8 mg/Kg) were tested (Figure 13). Similarly, two different doses of muMNACEP and mlgGl were tested, 1 mg/Kg and 8mg/Kg (Figure 14). All treatments were administered i.p. 24hours prior to intradermal injection with rhNGF. Systemic pre-treatment of 8 and 3 mg/kg BXhVH5VLl N297A significantly prevented the development of hyperalgesia following rhNGF injection, as indicated by no significant difference between ipsilateral and contralateral paw withdrawal responses (Figure 13). Systemic pre-treatment with the murine parental antibody mMNACEP (8 mg/Kg) also prevented the development of rhNGF-induced hyperalgesia (Figure 14). However, the overall analgesic response of BXhVH5VLl N297A appeared to be better as compared with mMNACEP antibody. At the same dose, hyperalgesia in ipsilateral paw responses was always present following co-injection with the negative control mlgG1 and hlgG. Data are represented as means ± 95% CI, before (baseline) and following intradermal injection of 500ng rhNGF with respective treatments (at arrow). Significant reduction in paw withdrawal latency ipsilateral is indicated by '*' '**' (p Example 8 Further in vivo experiments that may be performed to further assay the antibodies/derivatives of the present invention are set out below: Formalin test Mice are pretreated with the antibody/derivative intraperitoneally and 18 hours later are injected in the right dorsal footpad with 5% Formalin. Licking time (time spent licking the injured paw) is measured for up to 1 hour. Chronic constriction injury test Mice are subject to surgical constriction of sciatic nerve, in order to induce a neuropathic allodynia. Animals are then treated with antibody/derivative and withdrawal response to a mechanical stimulus localized to the injured limb versus the contralateral limb is measured. Arthritis model Rats are injected with complete Freund's adjuvant at the tail base intradermally. Approximately three weeks late they develop a systemic poly arthritis characterized by joint pain. Animals are treated with the antibody/derivative and the analgesic effect is evaluated by the vocalization assay consisting of measurement of intensity of vocalization upon gentle manipulation of the joints. Monkey carrageenan induced pain model Rhesus macaques are pretreated intravenously with the antibody/derivative. The following day, animals are injected subcutaneously with carrageenan in the tail. Withdrawal time from a heat stimulus is measured. General Points Unless the context indicates otherwise, the following general points apply: 1) All references discussed herein are deemed to be incorporated by reference. 2) The term "comprises" is non-limiting in that it covers "including" as well as "consisting of. Thus the word 'comprises' and variations such as 'comprise' and 'comprising' will be understood to imply the inclusion of a stated integer, step, group of integers or group of steps but not to the exclusion of any other integer, step, group of integers or group of steps. 3) Equivalents of aspects of the invention discussed herein are considered to be within the scope of the invention, even if the equivalents are not specifically discussed. SEQUENCE LISTING BioXell S.p.A. Benigni, Fabio d'Ambrosio, Daniele Antibodies and derivatives thereof BXL-P824PCT US 61/025,995 2008-02-04 23 Patentln version 3.5 1 123 PRT Homo sapiens 1 Glu Val Lys Leu Leu Glu Ser Gly GJy Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Val Arg Gln Thr Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 2 123 PRT Homo sapiens 2 Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Glrt Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Val Arg Gln Thr Pro Gly Lys Arg Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 3 123 PRT Homo sapiens 3 Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 4 123 PRT Homo sapiens 4 Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Val Arg Gln Thr Pro Gly Lys Arg Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr ;lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 5 123 PRT Homo sapiens 5 Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Tip Ala Arg Gin Ala Pro Gly Lys Gly Leu Glu Tip Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Phe Gly Asn Asp Phe Phe Phe Pro Met Asp Arg 100 105 110 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 6 123 PRT Homo sapiens 6 Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Ala Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 7 106 PRT Homo sapiens 7 Glu lle Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly lle Pro Ala Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp Phe Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Phe Ala Val Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gln Gly Thr Lys Leu Glu lle Lys 100 105 8 106 PRT Homo sapiens 8 Glu lle Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly lle Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp Phe Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gln Gly Thr Lys Leu Glu lle Lys 100 105 9 106 PRT Homo sapiens 9 Gln He Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Arg AJa Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly He Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Phe Tyr Thr Leu Thr lle Ser Ser Leu Glu Pro GIu 65 70 75 80 Asp Phe Ala Val Tyr Tyr Cys His Gpn Tpp Ser Ser Tyr Pro Tlp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Leu Glu lle Lys 100 105 10 106 PRT Homo sapiens 10 Gln lle Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 . Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Tlp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly lle Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp Phe Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Tlp Ser Ser Tyr Pro Tlp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Leu Glu lle Lys 100 105 11 106 PRT Homo sapiens II Gln lle Val Leu Thr Gin Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly lln Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly He Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp Tyr Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gln Gly Thr Lys Leu Glu He Lys 100 105 12 106 PRT Homo sapiens 12 Glu lle Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Glu Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Tlp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly lle Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Phe Tyr Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Leu Glu lle Lys 100 105 13 106 PRT Homo sapiens 13 Gln He Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly lle Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Phe Tyr Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Leu Glu lle Lys 100 105 14 106 PRT Homo sapiens 14 Gln lle Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Glu Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Arg Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly lle Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Phe Tyr Thr Leu Thr lle Ser Ser Leu Glu Pro Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Leu Glu lle Lys 100 105 15 123 PRT Musmusculus 15 Glu Val Lys Leu Met Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 3 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Ala Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gln Thr Ser Val Thr Val Ser Ser 115 120 16 106 PRT Mus musculus 16 Asp lle Val Leu Ser Gln Ser Pro Ala lle Met Ser Ala Ser Leu Gly 15 10 15 Glu Glu lle Thr Leu Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Leu Leu lle Thr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Phe Tyr Ser Leu Thr lle Ser Ser Val Glu Ala Glu 65 70 75 80 Asp Ala Ala Asp Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Leu GIu lle Lys 100 105 17 123 PRT Homo sapiens 17 Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Ala Arg Grn Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Phe Gly Asn Asp Phe Phe Phe Pro Met Asp Arg 100 105 110 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 18 106 PRT Homo sapiens 18 Asp lle Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 15 10 15 Asp Arg Val Thr lle Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gin Lys Pro Gly Gln Ala Pro Lys Leu Leu lle Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp Tyr Thr Leu Thr lle Ser Ser Leu Gln Pro Glu 65 70 75 80 Asp Val Ala Thr Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Trp Thr 85 90 95 Phe Gly Gly Gly Thr Lys Val Glu lle Lys 100 105 19 98 PRT Homo sapiens 19 Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30 Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ser Ala lle Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys 20 15 PRT Homo sapiens 20 Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 15 10 15 21 95 PRT Homo sapiens 21 Glu lle Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 15 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 20 25 30 Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu lle 35 40 45 Tyr Asp Ala Ser Asn Arg Ala Thr Gly lle Pro Ala Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr lle Ser Ser Leu Glu Pro 65 70 75 80 Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro 85 90 95 22 12 PRT Homo sapiens 22 Trp Thr Phe Gly Gln Gly Thr Lys Val Glu lle Lys 1 5 10 23 453 PRT Homo sapiens 23 Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 15 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 20 25 30 Thr Met Ser Trp Val Arg Gln Thr Pro Gly Lys Arg Leu Glu Trp Val 35 40 45 Ala Tyr lle Ser Lys Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr lle Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Ala Met Tyr Gly Asn Asp Phe Phe Tyr Pro Met Asp Tyr 100 105 110 Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly 115 120 125 Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 130 135 140 Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 145 150 155 160 Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 165 170 175 Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 180 185 190 Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr lle Cys Asn Val 195 200 205 Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 210 215 220 Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 225 230 235 240 Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 245 250 255 Leu Met lle Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 260 265 270 Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 275 280 285 Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gin Tyr Ala Ser 290 295 300 Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 305 310 315 320 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 325 330 335 Pro lle Glu Lys Thr lle Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 340 345 350 Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 355 360 365 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp lle Ala 370 375 380 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 385 390 395 400 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 405 410 415 Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 420 425 430 Val Met His Glu Gly Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 435 440 445 Leu Ser Pro Gly Lys 450 Claims 1. An anti-TrkA antibody that comprises: a) a variable heavy chain comprising a sequence selected frorn any of BXhVHl, BXhVH2, BXhVH3, BXhVH4, BXhVH5 or HuVHWOv as shown in Figure la; and/or b) a variable light chain comprising a sequence selected from any of BXhVLl, BXhVL2, BXhVL3, BXhVL4, BXhVL5, BXhVL6, BXhVL7 or BXhVL8 as shown in Figure lb or a derivative of said antibody wherein the derivative is capable of binding TrkA wherein the antibody or derivative has at least 85% humsnisation when measured over the total framework regions of the variable region of at least one of its chains (excluding the associated CDR regions). 2. An antibody or derivative according to claim 1 wherein the antibody comprises one of the following combinations of light and heavy chains: BXhVHlVL1, BXhVHlVL2, BXhVHlVL3, BXhVHlVL4, BXhVHIVLS, BXhVHlVL6, BXhVHlVL7, BXhVHlVL8, BXWH2VL1, BXhVH2VL2, BXhVH2VL3, BXhVH2VL4, BXhVH2VL5, BXhVH2VL6, BXhVH2VL7, BXhVH2VL8, BXhVH3VLl, BXhVH3VL2, BXhVH3VL3, BXhVH3VL4, BXhVH3VL5; BXhVH3VL6, BXhVH3VL7, BXhVH3VL8, BXhVH4VLl, BXhVH4VL2s BXhVH4VL3, BXhVH4VL4, BXhVH4VL5; BXhVH4VL6, BXhVH4VL7, BXhVH4VL8, BXhVHSVLl, BXhVH5VL2, BXhVH5VL3, BXhVH5VL4, BXhVH5VL5, BXhVH5VL6, BXhVH5VL7, BXhVH5VL8, or HuVHWOv/HuVLWO or a derivative of said antibody; wherein the derivative is capable of binding TrkA. 3. An antibody or derivative according to claim 1 or claim 2 wherein the antibody comprises one of the following combinations of light and heavy chains: BXhVH3VL3, BXhVH5VLl, or BXhVH5VL3. 4. An antibody or derivative according to any preceding claim that comprises one or more of CDR regions selected from the regions underlined in Figures la & lb or from variants thereof having up to two amino acid changes per underlined region, 5. An antibody or derivative according to any preceding claim that comprises six CDR regions. 6. An antibody or derivative according to any preceding claim that has a plurality of framework regions selected from the non-underlined sequences shown in Figures la and/or lb or from variants thereof that have at least 75% sequence identity with the non-underlined regions. 7. An antibody or derivative according to claim 6 wherein said sequence identity is at least 85%. 8. An antibody or derivative according to claim 6 wherein said sequence identity is at least 95%. 9. An antibody or derivative according to claim 6 wherein the variants have no greater a percentage of murine residues present in the framework regions than are present in the corresponding non-underlined regions shown in Figures la and/or lb. 10. An antibody or derivative according to any preceding claim that binds with greater affinity to TrkA than to Trk B. 11. An antibody or derivative according to any preceding claim that is capable of blocking or reducing the binding of NGF to the TrkA receptor. 12. An antibody or derivative according to any preceding claim that is capable of blocking or reducing one or more biological activities that would otherwise be induced by the binding of NGF to the TrkA receptor. 13. An antibody or derivative according to any preceding claim comprising a non-rodent derived constant region. 14. An antibody or derivative according to any preceding claim comprising a human constant region or a constant region with at least 75% sequence identity with a human constant region. 15. An antibody or derivative according to any preceding claim comprising a human IgG constant region or a constant region with at least 75% sequence identity therewith. 16. An antibody or derivative thereof comprising a constant region that comprises: a) the sequence shown in Figure 6 for the heavy chain that begins with the underlined A and terminates with the final K or a sequence with at least 75% sequence identity therewith; and /or b) the sequence shown in Figure 6 for the light chain that begins with the underlined R and terminates with the final C, or a sequence with at least 75% sequence identity therewith. 17. An antibody or derivative according to claim 16 wherein position 297 of the heavy chain constant region is mutated from N to A (N297A) 18. A derivative according to any preceding claim comprising a constant region that has one or more amino acid changes relative to a human immunoglobulin constant region that prevent/reduce one or more of the following, a) activation of complement b) complement-mediated lysis c) activation of T cells d) binding to an Fc receptor. 19. An antibody or derivative according to any preceding claim that provides an OD450/630 nm value of greater than 0.1 in the TrkA-IgG binding assay illustrated by Figure 2. 20. An antibody or derivative according to any preceding that provides an increase in FACS staining of TFI cells in the FACS based assay described in the examples that is an increase is an increase of over 1,0 fold. 21. An antibody or derivative according to any preceding claim that has a Kp value of less than 4.18 x 10"s M in respect of TrkA-IgG binding in an assay as described in the Examples. 22. An antibody or derivative according to any preceding claim that has at least 90% humanisation when measured over the total framework regions of the variable region of at least one chain (excluding the associated CDR regions). 23. An antibody or derivative according to any preceding claim that has at least 95% humanisation when measured over the total framework regions of the variable region of at least one chain (excluding the associated CDR regions). 24. An antibody or derivative according to claim 1 or any of claims 21 to 22 that has at least the percentage humanisation given in any of said claims in respect of the framework regions for both the light and heavy chains. 25. A derivative of an antibody according to any preceding claim wherein the derivative is a) a fragment of said antibody b) a fragment multimer c) a fusion product of said antibody fragment or fragment multimer and another moiety d) a variant of said antibody, fragment, fragment multimer, or fusion product, having at least 75% sequence identity therewith. 26. A derivative according to claim 24 wherein the derivative is a fusion product and another moiety is an agent selected from the following: a diagnostic agent, a therapeutic agent, a marking agent, an agent that increases the half life and/or that reduces the immunogenicity of said derivative in a human host. 27. A derivative thereof according to claim 25 wherein the therapeutic agent is a cytotoxin. 28. An antibody or derivative thereof according to any preceding claim that is PEGylated. 29. An antibody or derivative according to any preceding claim in immobilised form. 30. An antibody or derivative according to any of claims 1 to 28; for use in medicine 31. An antibody or derivative according to any of claims 1 to 28; for use in the treatment of pain. 32. An antibody or derivative according to any of claims 1 to 28; for use in the treatment of chronic pain. 33. An antibody or derivative according to any of claims 1 to 28; for use in the treatment of acute pain. 34. An antibody or derivative according to any of claims 1 to 28; for use in the treatment of pain associated with one or more of the following: pancreatitis, kidney stones, endometriosis, IBD, Crohn's disease, post surgical adhesions , gall bladder stones, headaches, dysmenorrhea, musculoskeletal pain, sprains, visceral pain, ovarian cysts, prostatitis, cystitis, interstitial cystitis, post-operative pain, migraine, trigeminal neuralgia, pain from burns and/or wounds, pain associated with trauma, neuropathic pain, pain associated with musculoskeletal diseases, rheumatoid arthritis, osteoarthritis, ankylosing spondilitis, periarticular pathologies, oncological pain, pain from bone metastases, HIV infection. 35. An antibody or derivative according to any of claims 1 to 28; for use in the treatment of cancer, a neuronal disorder (e.g. a neurodegenerative disorder), Alzheimer's disease, diabetes mellitus, a viral disorder, an HIV mediated disorder, leprosy, or an inflammatory disorder. 36. An antibody or derivative according to any of claims 30 to 34; whereas the treatment involves ADCC. 37. A combination of an antibody or derivative according to any of claims 1 to 28 and an analgesic, for simultaneous, sequential or concerted administration in medicine. 38. A combination of an antibody or derivative according to any of claims 1 to 28 and NGF, for simultaneous, sequential or concerted administration in medicine. 39. A combination of an antibody or derivative according to any of claims 1 to 28 and a further anti-TrkA antibody or derivative thereof, for simultaneous, sequential or concerted administration in medicine. 40. A pharmaceutical composition comprising an antibody or derivative thereof according to any of claims 1 to 28 and a pharmaceutically acceptable carrier or excipient. 41. A pharmaceutical composition comprising an antibody according to any of claims 1 to 28 and another pharmaceutically active agent. 42. A pharmaceutical composition according to claim 40; wherein said another agent is one or more of: a) an analgesic agent b) another anti-TrkA antibody or derivative thereof c)NGF d) an anti-cancer agent. 43. An antibody or derivative thereof according to any of claims 1 to 28 for use in diagnosis or prognosis. 44. An antibody or derivative thereof for use in the diagnosis or prognosis of a condition involving aberrant expression of TrkA or an aberrant activity involving TrkA. 45. An antibody or derivative thereof for use in the diagnosis or prognosis of any of the diseases or disorders specified in claims 30 to 34. 46. A method comprising obtaining a biological sample and administering an antibody or derivative according to any of claims 1 to 28 thereto. 47. A method according to claim 45 further comprising comparing the results for binding of said antibody or derivative to said sample with a positive or negative control sample. 48. A polypeptide that consists of or comprises a variable light chain of an antibody or derivative thereof according to any of claims 1 to 28. 49. A polypeptide that consists of or comprises a variable heavy chain of an antibody or derivative thereof as described in any of claims 1 to 28. 50. A polypeptide according to claim 47 or claim 48 that further comprises one or more human constant regions. 51. A nucleic acid encoding an antibody or derivative thereof according to any of claims 1 to 28 or encoding a polypeptide according to any of claims 47 to 49. 52. A vector comprising a nucleic acid according to claim 50. 53. An expression system that expresses an antibody or derivative thereof according to any of claims 1 to 28, or a polypeptide according to any of claims 47 to 49 or that can be induced to provide such expression. 54. A non-human transgenic mammal that expresses an antibody or derivative thereof according to any of claims 1 to 28, or a polypeptide according to any of claims 47 to 49 or that can be induced to provide such expression. 55. A kit of parts comprising an antibody or derivative according to any one of claims 1 to 28 together with instructions directing the use thereof by a subject as an analgesic.

Documents:

1861-mumnp-2010- claims.doc

1861-MUMNP-2010--CORRESPONDENCE(15-12-2010).pdf

1861-MUMNP-2010--FORM 1(15-12-2010).pdf

1861-mumnp-2010-abstract.doc

1861-mumnp-2010-abstract.pdf

1861-MUMNP-2010-ANNEXURE-A(15-04-2013).pdf

1861-MUMNP-2010-CLAIMS(AMENDED)-(13-1-2014).pdf

1861-MUMNP-2010-CLAIMS(AMENDED)-(15-4-2013).pdf

1861-MUMNP-2010-CLAIMS(MARKED COPY)-(13-1-2014).pdf

1861-MUMNP-2010-CLAIMS(MARKED COPY)-(15-4-2013).pdf

1861-mumnp-2010-claims.pdf

1861-MUMNP-2010-CORRESPONDENCE(11-10-2013).pdf

1861-MUMNP-2010-CORRESPONDENCE(15-04-2013).pdf

1861-MUMNP-2010-CORRESPONDENCE(15-10-2013).pdf

1861-MUMNP-2010-CORRESPONDENCE(15-12-2010).pdf

1861-MUMNP-2010-CORRESPONDENCE(15-9-2011).pdf

1861-MUMNP-2010-CORRESPONDENCE(17-7-2012).pdf

1861-MUMNP-2010-CORRESPONDENCE(20-2-2013).pdf

1861-MUMNP-2010-CORRESPONDENCE(23-9-2010).pdf

1861-MUMNP-2010-CORRESPONDENCE(25-1-2012).pdf

1861-MUMNP-2010-CORRESPONDENCE(28-3-2011).pdf

1861-mumnp-2010-correspondence.pdf

1861-mumnp-2010-description(complete).pdf

1861-MUMNP-2010-DRAWING(13-1-2014).pdf

1861-mumnp-2010-drawing.pdf

1861-mumnp-2010-form 1.pdf

1861-MUMNP-2010-FORM 13(15-04-2013).pdf

1861-mumnp-2010-form 13.pdf

1861-MUMNP-2010-FORM 18(23-9-2010).pdf

1861-mumnp-2010-form 2(title page).pdf

1861-mumnp-2010-form 2.doc

1861-mumnp-2010-form 2.pdf

1861-MUMNP-2010-FORM 26(15-12-2010).pdf

1861-MUMNP-2010-FORM 26(15-4-2013).pdf

1861-MUMNP-2010-FORM 3(11-10-2013).pdf

1861-MUMNP-2010-FORM 3(15-4-2013).pdf

1861-MUMNP-2010-FORM 3(15-9-2011).pdf

1861-MUMNP-2010-FORM 3(17-7-2012).pdf

1861-MUMNP-2010-FORM 3(25-1-2012).pdf

1861-MUMNP-2010-FORM 3(28-3-2011).pdf

1861-mumnp-2010-form 3.pdf

1861-mumnp-2010-form 5.pdf

1861-mumnp-2010-form pct-ib-306.pdf

1861-MUMNP-2010-MARKED COPY(15-04-2013).pdf

1861-mumnp-2010-marked copy.pdf

1861-MUMNP-2010-OFFICE ACTION(15-10-2013).pdf

1861-MUMNP-2010-OTHER DOCUMENT(15-4-2013).pdf

1861-MUMNP-2010-PETITION UNDER RULE 137(15-4-2013).pdf

1861-MUMNP-2010-PETITION UNDER RULE-137(20-2-2013).pdf

1861-MUMNP-2010-REPLY TO EXAMINATION REPORT(15-4-2013).pdf

1861-MUMNP-2010-REPLY TO HEARING(13-1-2014).pdf

1861-MUMNP-2010-SEQUENCE LISTING(15-4-2013).pdf

1861-mumnp-2010-substitute sheet.pdf

1861-mumnp-2010-wo international publication report a1.pdf

abstract1.jpg

Address change - Copy.pdf

Address change.pdf

Amended.pdf

form 3 -22-01-2014.pdf

Form-13 & Annexure.pdf

letter and docs.pdf

letter.pdf

Marked Up Amended.pdf

Petition Form-3.pdf

petition-20140121.pdf

petition.pdf

Relevant Document.pdf