| Title of Invention | "A METHOD FOR MAKING A RECOMBINANT PROTEIN" |
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| Abstract | The present invention deals with examination of the alterability of part, or whole, of the surfaces of beta sheet-based protein structures, focusing especially on enzymes. The alteration is done by supplanting/transplanting a part, or whole, of the surface of one protein onto the surface of a homologous protein of superimposable popypeptide backbone, by exploiting the structural features of beta sheets to alter only the regions of the surface involved in substrate/ligand binding and catalysis. The transplantation involves replacement of a selected set of non-contiguous residues constituting the surface regions desired to be altered in one enzyme/protein, by a set of non-contiguous residues located at analogous positions in the other enzyme/protein, in a manner that is likely to facilitate folding and function of the new chimera formed by combining residues from both enzymes/proteins. The present invention also deals with using this surface engineering approach to selectively combine enzyme/protein characteristics from different domains of life that are not ordinarily combined by natural evolution, such as the creation of chimera that retains the bulk of the thermostable scaffold of a thermophile enzyme onto which the active surface of a mesophile homolog is transplanted, so as to create a thermo-stable protein with meso-active functional characteristics of pH and temperature of optimal function. |
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2411-del-2006-Abstract-(15-07-2013).pdf
2411-del-2006-Claims-(15-07-2013).pdf
2411-del-2006-Claims-(20-12-2012).pdf
2411-del-2006-Correspondence Others-(20-03-2013).pdf
2411-del-2006-Correspondence Others-(20-12-2012).pdf
2411-del-2006-correspondence-others (17-03-2008).pdf
2411-del-2006-Correspondence-Others-(03-07-2013).pdf
2411-del-2006-Correspondence-Others-(15-07-2013).pdf
2411-DEL-2006-Correspondence-Others-(18-12-2009).pdf
2411-DEL-2006-Correspondence-Others-(23-03-2010).pdf
2411-del-2006-correspondence-others.pdf
2411-del-2006-description (provisional).pdf
2411-del-2006-form-18 (17-03-2008).pdf
2411-DEL-2006-Form-3-(18-12-2009).pdf
2411-del-2006-Form-3-(20-03-2013).pdf
2411-del-2006-Form-3-(20-12-2012).pdf
2411-DEL-2006-Form-3-(23-03-2010).pdf
2411-del-2006-Petition-137-(20-12-2012).pdf
| Patent Number | 257848 | |||||||||||||||||||||||||||
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| Indian Patent Application Number | 2411/DEL/2006 | |||||||||||||||||||||||||||
| PG Journal Number | 46/2013 | |||||||||||||||||||||||||||
| Publication Date | 15-Nov-2013 | |||||||||||||||||||||||||||
| Grant Date | 12-Nov-2013 | |||||||||||||||||||||||||||
| Date of Filing | 06-Nov-2006 | |||||||||||||||||||||||||||
| Name of Patentee | COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH | |||||||||||||||||||||||||||
| Applicant Address | ANUSANDHAN BHAWAN, RAFI MARG. NEW DELHI-110 001, INDIA | |||||||||||||||||||||||||||
Inventors:
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| PCT International Classification Number | C07K14/205; C07K14/28 | |||||||||||||||||||||||||||
| PCT International Application Number | N/A | |||||||||||||||||||||||||||
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