Title of Invention	" ß-AMYLOID MODULATOR COMPOUND"
Abstract	A compound having the structure: N-methyl-(D-Lue-D-Val-D-Phe-D-Phe-D-Lue)-NH2.

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The present invention relates to a ß-amyloid modulator compound. Background of the Invention Alzheimer's disease (AD), first described by the Bavarian psychiatrist Alois Alzheimer in 1907, is a progressive neurological disorder that begins with short term memory loss and proceeds to disorientation, impairment of judgment and reasoning and, ultimately, dementia. The course of the disease usually leads to death in a severely debilitated, immobile state between four and 12 years after onset' AD has been estimated to afflict 5 to 11 percent of the population over age 65 and as much as 47 percent of the population over age 85 The societal cost for managing AD is upwards of 80 billion dollars annually, primarily due to the extensive custodial care required for AD patients Moreover, as adults born during the population boom of the 1940's and 1950's approach the age when AD becomes more prevalent, the control and treatment of AD will become an even more significant health care problem Currently, there is no treatment that significantly retards the progression of the disease For reviews on AD, see Selkoe, DJ. Sci Amer. .November 1991, pp. 68-78; and Yankner, B.A. et al (1991) N Eng J Med 325.1849-1857. It has recently been reported (Games et al (1995) Nature 373:523-527) that an Alzheimer-type neuropathology has been created in transgenic mice. The transgenic mice express high levels of human mutant amyloid precursor protein and progressively develop many of the pathological conditions associated with AD. Pathologically, AD is characterized by the presence of distinctive lesions in the victim's brain. These brain lesions include abnormal intracellular filaments called neurofibrillary tangles (NTFs) and extracellular deposits of amyloidogenic proteins in senile, or amyloid, plaques. Amyloid deposits are also present in the walls of cerebral blood vessels of AD patients. The major protein constituent of amyloid plaques has been identified as a 4 kilodalton peptide called (3-amyloid peptide (ß-AP)(Glenner, G.G and Wong, C.W. (1984) Biochem. Biophys Res Commun. K20:885-890; Masters, C. el al (1985) Proc. Natl Acad Sci USA 82 4245-4249) Diffuse deposits of ß-AP are frequently observed in normal adult brains, whereas AD brain tissue is characterized by more compacted, dense-core ß-amyloid plaques. (See e g., Davies, L et al. (1988) Neurology 38.1688-1693) These observations suggest that ß-AP deposition precedes, and contributes to, the destruction of neurons that occurs in AD. In further support of a direct pathogenic role for ß-AP, ß-amyloid has been shown to be toxic to mature neurons, both in culture and in vivo. Yankner, B.A et al (1989) Science 245:417-420, Yankner, B.A. et al. (1990) Proc Natl Acad. Sci USA 87:9020-9023; Roher, A.E. et al (1991) Biochem. Biophys. Res. Commun J74:572-579; Kowall, N.W et al. (1991) Proc Natl Acad. Sci USA 88:7247-7251. Furthermore, patients with hereditary cerebral hemorrhage with amyloidosis-Dutch-type (HCHWA-D), which is characterized by diffuse ß-amyloid deposits within the cerebral cortex and cerebrovasculature, have been shown to have a point mutation that leads to an ammo acid substitution within ß-AP Levy, E. et al (1990) Science 248:1124-1126 This observation demonstrates that a specific alteration of the ß-AP sequence can cause ß-amyloid to be deposited. Natural ß-AP is derived by proteolysis from a much larger protein called the amyloid precursor protein (APP). Kang, J. et al (1987) Nature 325:733; Goldgaber, D etal. (1987) Science 235:877; Robakis, N K et al (1987) Proc Natl Acad. Sci USA 84:4190; Tanzi, R.E. et al. (1987) Science 235.880 The APP gene maps to chromosome 21, thereby providing an explanation for the ß-amyloid deposition seen at an early age in individuals with Down's syndrome, which is caused by trisomy of chromosome 21. Mann, D.M. et al. (1989) Neuropathol Appl Neurobiol j_5:317, Rumble, B. et al (1989) AT. Eng J Med. 320:1446 APP contains a single membrane spanning domain, with a long ammo terminal region (about two-thirds of the protein) extending into the extracellular environment and a shorter carboxy-terminal region projecting into the cytoplasm Differential splicing of the APP messenger RNA leads to at least five forms of APP, composed of either 563 amino acids (APP-563), 695 amino acids (APP-695), 714 amino acids (APP-714), 751 amino acids (APP-751) or 770 ammo acids (APP-770). Within APP, naturally-occumng ß amyloid peptide begins at an aspartic acid residue at amino acid position 672 of APP-770 Naturally-occurring ß-AP derived from proteolysis of APP is 39 to 43 amino acid residues in length, depending on the carboxy- terminal end point, which exhibits heterogeneity. The predominant circulating form of J3-AP in the blood and cerebrospinal fluid of both AD patients and normal adults is j31 -40 ("short 0"). Seubert, P. et al. (1992) Nature 359:325; Shoji, M. et al (1992) Science 258:126. However, (31-42 and pi-43 ("long P") also are forms in ß-amyloid plaques. Masters, C. et al. (1985) Proc. Natl Acad. Sci. USA 82:4245; Miller, D. et al (1993) Arch. Biochem. Biophys. 301:41; Mori, H. etal (1992) J. Biol Chem. 267:17082. Although the precise molecular mechanism leading to P-APP aggregation and deposition is unknown, the process has been likened to that of nucleation-dependent polymerizations, such as protein crystallization, microtubule formation and actin polymerization. See e.g., Jarrett, J.T. and Lansbury, P.T. (1993) Cell 73:1055-1058. In such processes, polymerization of monomer components does not occur until nucleus formation. Thus, these processes are characterized by a lag time before aggregation occurs, followed by rapid polymerization after nucleation. Nucleation can be accelerated by the addition of a "seed" or preformed nucleus, which results in rapid polymerization. The long ß forms of ß-AP have been shown to act as seeds, thereby accelerating polymerization of both long and short ß-AP forms. Jarrett, J.T. et al (1993) Biochemistry 32:4693. In one study, in which amino acid substitutions were made in ß-AP, two mutant ß peptides were reported to interfere with polymerization of non-mutated ß-AP when the mutant and non-mutant forms of peptide were mixed. Hilbich. C. et al. (1992) J Mol. Biol. 228:460-473. Equimolar amounts of the mutant and non-mutant (i e , natural) p amyloid peptides were used to see this effect and the mutant peptides were reported to be unsuitable for use in vivo. Hilbich, C. et al (1992), supra. Summary of the Invention This invention pertains to compounds, and pharmaceutical compositions thereof, that can bind to natural ß amyloid peptides (ß-AP), modulate the aggregation of natural ß-AP and/or inhibit the neurotoxicity of natural ß-APs. The compounds are modified in a manner which allows for increased biostability and prolonged elevated plasma levels. The ß-amyloid modulator compounds of the mvention comprise apeptidic structure, preferably based on ß-amyloid peptide, that is composed entirely of D-amino acids In various embodiments, the peptidic structure of the modulator compound comprises a D-amino acid sequence corresponding to a L-amino acid sequence found within natural ß-AP, a D-amino acid sequence which is an inverso isomer of an L-amino acid sequence found within natural ß-AP, a D-amino acid sequence which is a retro-inverso isomer of an L-amino acid sequence found within natural ß-AP, or a D-amino acid sequence that is a scrambled or substituted version of an L-amino acid sequence found within natural ß-AP. Preferably, the D-amino acid peptidic structure of the modulator is designed based upon a subregion of natural ß-AP at positions 17-21 (Aß17.2o and Aß17.2], respectively), which has the amino acid sequences Leu-Val-Phe-Phe-Ala (SEQ ID NO:4). In preferred embodiments, a phenylalanine in the compounds of the invention is substituted with a phenylalanine analogue Which is more stable and less prone to, for example, oxidative metabolism, or allows for increased brain levels of the compound. In yet another embodiment, a modulator compound of the invention includes a ß-amyloid peptide comprised of D-amino acids, L-amino acids or both, an inverso isomer of a ß-amyloid peptide, or a retro-inverso isomer of a ß-amyloid peptide which is attached to a hydrazine moiety, wherein the compound binds to natural ß-amyloid peptides or modulates the aggregation or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. A modulator compound of the invention preferably comprises 3-20 D-amino acids, more preferably 3-10 D-amino acids and even more preferably 3-5 D-amino acids The D-amino acid peptidic structure of the modulator can have free ammo-, carboxy-, or carboxy amide- termini. Alternatively, the amino-terminus, the carboxy-terminus or both may be modified. For example, an N-terminal modifying group can be used that enhances the ability of the compound to inhibit Aß aggregation. Moreover, the amino-and/or carboxy termini of the peptide can be modified to alter a pharmacokinetic property of the compound (such as stability, bioavailability, e.g., enhanced delivery of. the compound across the blood brain barrier and entry into the brain, and the like). Preferred amino-terrninal modifying groups include alkyl groups, e.g., methyl, ethyl, or isopropyl groups. Preferred carboxy-terminal modifying groups include amide groups, alkyl or aryl amide groups (e g, phenethylamide), hydroxy groups (i.e., reduction products of peptide acids, resulting in peptide alcohols), acyl amide groups, and acetyl groups. Still further, a modulator compound can be modified to label the compound with a detectable substance {e.g., a radioactive label) In certain preferred embodiments, the invention provides a compound having the structure: N,N-dimethyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2; N,N-dimethyl-(D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-methyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-ethyl-(Giy-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-isopropyl-(Giy-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;H-(D-Leu-D-VaI-D-Phe-D-Phe-D-Ala)-isopropylamide;H-(D-Leu-D-Val-D-Phe-D-Phe-D-Ala)-dimethylamide;N,N-diethyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N,N-diethyl-(D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH,;N,N-dimethyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N,N-dimethyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2,N,N-dimethyl-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH2;H-(Gly-D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N-ethyl-(Gly-D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N-ethyl-(Gly-D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH,;N,N-dimethyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N,N-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N-propyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2, N,N-diethyl-(Gly-D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;H-(D-Ue-D-Val-D-Phe-D-Phe-D-Ile)-NH2; H-(D-Ile-D-Val-D-Phe-D-Phe-D-Ala-)-NH2; H-( D-Ile- D-Ile-D-Phe-D-Phe- D-Ile)-NH2; H-(D-Nle-D-Val-D-Phe-D-Phe-D-Ala-)-NH2; H-(D-Nle-D-Val-D-Phe-D-Phe-D-Nle)-NH2; 1 -piperidine-acety]-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH:, l-piperidine-acetyl-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH2;H-D-Leu-D-Val-D-Phe-D-Phe-D-Leu-isopropylamide; H-D-Leu-D-Phe-D-Phe-D-Val-D-Leu-isopropylarnide; H-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-methylamide;H-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-methylamide;H-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-OH;N-methyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;H-(D-Leu-D-Val-D-Phe-D-Cha-D-Leu)-NH2;H-(D-Leu-D-Val-D-Phe-D-[ß-F]Phe-D-Leu)-NH2;H-(D-Leu-D-Val-D-Phe-D-[F5]Phe-D-Leu)-NH2; H-(D-Leu-D-Phe-D-Cha-D-Val-D-Leu)-NH2; H-(D-Leu-D-Phe- D-f-FJPhe-D-Val-D-Leu)-NH2; H-(D-Leu-D-Phe- D-[F5]Phe-D-Val-D-Leu)-NH2; H-(D-Leu-D-Phe-D-Lys-D-Val-D-Leu)-NH2;H-(D-Leu-D-Cha-D-Phe-D-VaI-D-Leu)-NH2;H-(D-Leu-D-Cß-F]Phe-D-Phe-D-Val-D-Leu)-NH2;H-(D-Leu-D-[F5]Phe-D-Phe-D-Val-D- Leu)-NH2; H-(D-Leu- D-Lys-D-Phe-D-Val-D-Leu)-NH2; H-(D-Leu-D-Cha-D-Cha-D-Val-D-Leu)-NH2; H-(D-Leu- D-[/>F]Phe-D-[p-F]Phe-D-Val-D-Leu)-NH2; H-(D-Leu-D-[F5]Phe-D-[F5]Phe-D-Val-D-Leu)-NH2; H-(D-Leu- D-Lys- D-Lys-D-Val-D-Leu)-NH2; N-methyl-(D-Leu-D-Val-D-Phe-D-Cha-D-Leu)-NH2;N-methyl-(D-Leu-D-Val-D-Phe-D-[ß-F]Phe-D-Leu)-NH2;N-methyl-(D-Leu-D-Val-D-Phe-D-[F5]Phe-D-Leu)-NH2;H-D-Leu-D-Val-D-Phe-NH-(H-D-Leu-D-Val-D-Phe-)NH;H-D-Leu-D-Val-D-Phe-NH-NH-COCH3; and H- D-Leu-D-Val-D-Phe-NH-NH2. Particularly preferred compounds of the invention are set forth in the Examples. Another aspect of the invention pertains to pharmaceutical compositions. Typically, the pharmaceutical composition comprises a therapeutically effective amount of a modulator compound of the invention and a pharmaceutically acceptable carrier Yet another aspect of the invention pertains to methods for inhibiting aggregation of natural (3-amyloid peptides. These methods compnse contacting the natural ß-amyloid peptides with a modulator compound of the invention such that aggregation of the natural (3-amyloid peptides is inhibited. Yet another aspect of the invention pertains to methods tor detecting the presence or absence of natural ß-amyloid peptides in a biological sample. These methods comprise contacting a biological sample with a compound of the invention, wherein the compound is labeled with a detectable substance, and detecting the compound bound to natural ß-amyloid peptides to thereby detect the presence or absence of natural ß-amyloid peptides in the biological sample. Still another aspect of the invention pertains to methods for treating a subject for a disorder associated with ß-amyloidosis. These methods comprise administering to the subject a therapeutically effective amount of a modulator compound of the invention such that the subject is treated for a disorder associated with ß-amyloidosis. Preferably, the disorder is Alzheimer's disease. Use of the modulators of the invention for therapy or for the manufacture of a medicament for the treatment of a disorder associated with ß-amyloidosis is also encompassed by the invention. Brief Description of the Drawings Figure 1 is a table depicting the results from a brain uptake assay. Figure 2 is a graph depicting the results from the fibril binding assay described m Example 2. Detailed Description of the Invention This invention pertains to compounds, and pharmaceutical compositions thereof, that can bind to natural ß-amyloid peptides, modulate the aggregation of natural ß amyloid peptides (ß-AP) and/or inhibit the neurotoxicity of natural ß-APs. The compounds are modified in a manner which allows for increased biostability and prolonged elevated plasma levels. A compound of the invention that modulates aggregation of natural ß-AP, referred to herein interchangeably as a ßamyloid modulator compound, a p amyloid modulator or simply a modulator, alters the aggregation of natural ß-AP when the modulator is contacted with natural ß-AP Thus, a compound of the invention acts to alter the natural aggregation process or rate for ß-AP, thereby disrupting this process Preferably, the compounds inhibit ß-AP aggregation. The compounds of the invention are characterized in that they comprise a peptidic structure composed entirely of D-ammo acid residues. This peptidic structure is preferably based on ß-amyloid peptide and can comprise, for example, a D-amino acid sequence corresponding to a L-amino acid sequence found within natural ß-AP, a D-amino acid sequence which is an inverso isomer of an L-amino acid sequence found within natural ß-AP, a D-amino acid sequence which is a retro-inverso isomer of an L-amino acid sequence found within natural ß-AP, or a D-amino acid sequence that is a scrambled or substituted version of an L-amino acid sequence found within natural ß-AP. In preferred embodiments, the phenylalanines in the compounds of the invention are substituted with phenylalanine analogues which are more stable and less prone to, for example, oxidative metabolism The invention encompasses modulator compounds comprising a D-amino acid peptidic structure having free amino-, carboxy-, or carboxy amide- termini, as well as modulator compounds in which the amino-terminus, the carboxy-terminus, and/or side chain(s) of the peptidic structure are modified The p amyloid modulator compounds of the invention can be selected based upon their ability to bind to natural ß-amyloid peptides, modulate the aggregation of natural ß-AP in vitro and/or inhibit the neurotoxicity of natural ß-AP fibrils for cultured cells (using assays described herein, for example, the neurotoxicity assay, the nucleation assay, or the fibril binding assay). Preferred modulator compounds inhibit the aggregation of natural ß-AP and/or inhibit the neurotoxicity of natural ß-AP. However, modulator compounds selected based on one or both of these properties may have additional properties in vivo that may be beneficial in the treatment of amyloidosis (J. S. Pachter et al (1998) "Api-40 induced neurocytopathic activation of human monocytes is blocked by Ap peptide aggregation inhibitors." Neurobiology of Aging (Abstracts -The 6,h International Conference on Alzheimer's Disease and Related Disorders, Amsterdam, 18-23 July 1998) 19, S128 (Abstract 540); R. Weltzein, A et al (1998) "Phagocytosis of Beta-Amyloid: A Possible Requisite for Neurotoxicity." J Neuroimmunology (Special Issue: Abstracts of the International Society of Neuroimmunology Fifth Intemational Congress, Montreal, Canada, 23-27 August 1998) 1998, 90, 32 (Abstract 162)). For example, the modulator compound may interfere with processing of natural ß-AP (either by direct or indirect protease inhibition) or by modulation of processes that produce toxic ß-AP, or other APP fragments, in vivo. Alternatively, modulator compounds may be selected based on these latter properties, rather than inhibition of Aß aggregation in vitro. Moreover, modulator compounds of the invention that are selected based upon their interaction with natural ß-AP also may interact with APP or with other APP fragments. Still further, a modulator compound of the invention can be characterized by its ability to bind to ß-amyloid fibrils (which can be determined, for example, by radiolabeling the compound, contacting the compound with ß-amyloid plaque and counting or detecting, e.g., by imaging, the compound bound to pathological forms of ß-AP, e.g., the plaque), while not significantly altering the aggregation of the ß-amyloid fibrils. Such a compound that binds efficiently to ß-amyloid fibrils while not significantly altering the aggregation of the ß-amyloid fibrils can be used, for example, to detect ß-amyloid fibrils (e g , for diagnostic purposes, as described further herein). It should be appreciated, however, that the ability of a particular compound to bind to ß-amyloid fibrils and/or modulate their aggregation may vary depending upon the concentration of the compound. Accordingly, a compound that, at a low concentration, binds to ß-amyloid fibrils without altering their aggregation may nevertheless inhibit aggregation of the fibrils at a higher concentration. All such compounds having the property of binding to ß-amyloid fibrils and/or modulating the aggregation of the fibrils are intended to be encompassed by the invention. As used herein, a "modulator" of ß-amyloid aggregation is intended to refer to an agent that, when contacted with natural p amyloid peptides, alters the aggregation of the natural p amyloid peptides. The term "aggregation of ß amyloid peptides" refers to a process whereby the peptides associate with each other to form a multimenc, largely insoluble complex. The term "aggregation" further is intended to encompass ß amyloid fibril formation and also encompasses ß-amyloid plaques. The terms natural ß-amyloid peptide natural ß-AP" and "natural Aß peptide", used interchangeably herein, are intended to encompass naturally occurring proteolytic cleavage products of the ß amyloid precursor protein (APP) which are involved m ß-AP aggregation and ß-amyloidosis. These natural peptides include ß-amyloid peptides having 39-43 amino acids (/. e , Aß (_39, Aß j.40, Aß j _4 j, Aß [_42 and Aß ].43). The amino-terminal amino acid residue of natural ß-AP corresponds to the aspartic acid residue at position 672 of the 770 ammo acid residue form of the amyloid precursor protein ("APP-770"). The 43 amino acid long form of natural ß-AP has the ammo acid sequence DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIAT (also shown in SEQ ID NO:l), whereas the shorter forms have 1-4 amino acid residues deleted from the carboxy-terrninal end. The amino acid sequence of APP-770 from position 672 (i.e., the ammo-terminus of natural ß-AP) to its C-terminal end (103 ammo acids) is shown in SEQ ID NO:2. The preferred form of natural ß-AP for use in the aggregation assays described herein is Aß].40 or Aß 1^2- In the presence of a modulator of the invention, aggregation of natural p amyloid peptides is "altered" or "modulated" The various forms of the term "alteration" or "modulation" are intended to encompass both inhibition of ß-AP aggregation and promotion of ß-AP aggregation. Aggregation of natural ß-AP is "inhibited" in the presence of the modulator when there is a decrease in the amount and/or rate of ß-AP aggregation as compared to the amount and/or rate of ß-AP aggregation in the absence of the modulator The various forms of the term "inhibition" are intended to include both complete and partial inhibition of ß-AP aggregation. Inhibition of aggregation can be quantitated as the fold increase in the lag time for aggregation or as the decrease in the overall plateau level of aggregation (i.e., total amount of aggregation), using an aggregation assay as described in the Examples. In various embodiments, a modulator of the invention increases the lag time of aggregation at least 1.2-fold, 1.5-fold, 1.8-fold. 2-fold, 2.5-fold, 3-fold, 4-fold or 5-fold, for example, when the compound is at a one molar equivalent to the ß-AP. In various other embodiments, a modulator of the invention inhibits the plateau level of aggregation at least 10%, 20%, 30%, 40 %, 50 %, 75 % or 100 %. A modulator which inhibits ß-AP aggregation (an "inhibitory modulator compound") can be used to prevent or delay the onset of ß-amyloid deposition. Preferably, inhibitory modulator compounds of the invention inhibit the formation and/or activity of neurotoxic aggregates of natural Aß peptide (i.e., the inhibitory compounds can be used to inhibit the neurotoxicity of ß-AP). Additionally, the inhibitory compounds of the invention can reduce the neurotoxicity of preformed ß-AP aggregates, indicating that the inhibitory modulators can either bind to preformed Aß fibrils or soluble aggregate and modulate their inherent neurotoxicity or that the modulators can perturb the equilibrium between monomeric and aggregated forms of ß~ Aß in favor of the non-neurotoxic form. Alternatively, in another embodiment, a modulator compound of the invention promotes the aggregation of natural Ap peptides. The various forms of the term "promotion" refer to an increase in the amount and/or rate of ß-AP aggregation m the presence of the modulator, as compared to the amount and/or rate of ß-AP aggregation in the absence of the modulator. Such a compound which promotes Aß aggregation is referred to as a stimulatory modulator compound Stimulatory modulator compounds may be useful for sequestering ß-amyloid peptides, for example in a biological compartment where aggregation of ß-AP may not be deleterious to thereby deplete ß-AP from a biological compartment where aggregation of ß-AP is deleterious. Moreover, stimulatory modulator compounds can be used to promote Aß aggregation in in vitro aggregation assays (e.g, assays such as those described in Example 2), for example in screening assays for test compounds that can then inhibit or reverse this Aß aggregation (z e., a stimulatory modulator compound can act as a "seed" to promote the formation of Aß aggregates). In a preferred embodiment, the modulators of the invention are capable of altering ß-AP aggregation when contacted with a molar excess amount of natural ß-AP. A "molar excess amount of natural ß-AP" refers to a concentration of natural ß-AP, in moles that is greater than the concentration, in moles, of the modulator. For example, if the modulator and ß-AP are both present at a concentration of 1 µM, they are said to be "equimolar", whereas if the modulator is present at a concentration of 1 µM and the ß-AP is present at a concentration of 5 µM, the ß-AP is said to be present at a 5-fold molar excess amount compared to the modulator. In preferred embodiments, a modulator of the invention is effective at altering natural ß-AP aggregation when the natural ß-AP is present at at least a 2-fold, 3-fold or 5-fold molar excess compared to the concentration of the modulator. In other embodiments, the modulator is effective at altering ß-AP aggregation when the natural ß-AP is present at at least a 10-fold, 20-fold, 33-fold, 50-fold, 100-fold, 500-fold or 1000-fold molar excess compared to the concentration of the modulator. As used herein, the term "ß amyloid peptide comprised entirely of D-amino acids", as used in a modulator of the invention, is intended to encompass peptides having an amino acid sequence identical to that of the natural sequence in APP, as well as peptides having acceptable amino acid substitutions from the natural sequence, but which is composed of D-amino acids rather than the natural L-amino acids present in natural ß-AP. Acceptable amino acid substitutions are those that do not affect and/or may improve the ability of the D-ammo acid-containing peptide to alter natural ß-AP aggregation. Moreover, particular ammo acid substitutions may further contribute to the ability of the peptide to alter natural ß-AP aggregation and/or may confer additional beneficial properties on the peptide (e.g, increased solubility, reduced association with other amyloid proteins, etc.). A peptide having an identical amino acid sequence to that found within a parent peptide but in which all L-amino acids have been substituted with all D-amino acids is also referred to as an "inverso" compounds. For example, if a parent peptide is Thr-Ala-Tyr, the inverso form is D-Thr-D-Ala-D-Tyr. As used herein, the term "retro-mverso isomer of a ß amyloid peptide", as used in a modulator of the invention, is intended to encompass peptides in which the sequence of the amino acids is reversed as compared to the sequence in natural ß-AP and all L-amino acids are replaced with D-ammo acids. For example, if a parent peptide is Thr-Ala-Tyr, the retro-inverso form is D-Tyr-D-Ala-D-Thr. Compared to the parent peptide, a retro-inverso peptide has a reversed backbone while retaimng substantially the original spatial conformation of the side chains, resulting in a retro-inverso isomer with a topology mat closely resembles the parent peptide. See Goodman et al. "Perspectives in Peptide Chemistry" pp. 283-294 (1981) See also U.S Patent No. 4,522,752 by Sisto for further description of "retro-inverso" peptides. Various additional aspects of the modulators of the invention, and the uses thereof, are described in further detail in the following subsections. I. Modulator Compounds In one embodiment, a modulator compound of the invention comprises a ß-amyloid peptide, the ß-amyloid peptide being comprised entirely of D-amino acids, wherein the compound binds to natural ß-amyloid peptides or modulates the aggregation or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. Preferably, the ß-amyloid peptide of the modulator is comprised of 3-20 D-amino acids, more preferably 3-10 D-amino acids, and even more preferably 3-5 D-amino acids. In preferred embodiments, a phenylalanine in the compounds of the invention is substituted with a phenylalanine analogue which is more stable and less prone to, for example, oxidative metabolism. In one embodiment, the ß-amyloid peptide of the modulator is amino-terminally modified, for example, with a modifying group comprising an alkyl group such as a CI -C6 lower alkyl group, e.g., a methyl, ethyl, or propyl group; or a cyclic, heterocyclic, polycyclic or branched alkyl group. Examples of suitable N-terminal modifying groups are descnbed further in subsection II below. In another embodiment, the ß-amyloid peptide of the modulator is carboxy-termmally modified, for example the modulator can comprise a peptide amide, a peptide alkyl or aryl amide (e.g., a peptide phenethylamide) or a peptide alcohol. Examples of suitable C-terminal modifying groups are described further in subsections II and III below. The ß-amyloid peptide of the modulator may be modified to enhance the ability of the modulator to alter ß-AP aggregation or neurotoxicity. Additionally or alternatively, ß-amyloid peptide of the modulator may be modified to alter a pharmacokinetic property of the modulator and/or to label the modulator with a detectable substance (described further in subsection III below). In another embodiment, a modulator compound of the invention comprises a retro-inverso isomer of a ß-amyloid peptide, wherein the compound binds to natural ß-amyloid peptides or modulates the aggregation or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. Preferably, the retro-inverso isomer of the ß-amyloid peptide is comprised of 3-20 D-amino acids, more preferably 3-10 D-amino acids, and even more preferably 3-5 D-amino acids. In preferred embodiments, the phenylalanines in the compounds of the invention are substituted with phenylalanine analogues which are more stable and less prone to, for example, oxidative metabolism. In one embodiment, the retro-inverso isomer is amino-terminally modified, for example, with a modifying group comprising an alkyl group such as a C1-C6 lower alkyl group, e g,a methyl, ethyl, or propyl group; or a cyclic, heterocyclic, polycyclic or branched alkyl group. Examples of suitable N-terminal modifying groups are described further in subsection II below. In another embodiment, the retro-inverso isomer is carboxy-termmally modified, for example with an amide group, an alkyl or aryl amide group (e g, phenethylamide) or a hydroxy group (i.e., the reduction product of a peptide acid, resulting in a peptide alcohol). Examples of suitable C-terminal modifying groups are described further in subsections II and III below. The retro-inverso isomer may be modified to enhance the ability of the modulator to alter (3-AP aggregation or neurotoxicity. Additionally or alternatively, the retro-inverso isomer may be modified to alter a pharmacokinetic property of the modulator and/or to label the modulator with a detectable substance (described further in subsection III below). In yet another embodiment, a modulator compound of the invention includes a ß-amyloid peptide comprised entirely or partially of D-amino acids, an inverso isomer of a ß-amyloid peptide, or a retro-inverso isomer of a ß-amyloid peptide which is attached to a hydrazine moiety, wherein the compound binds to natural ß-amyloid peptides or modulates the aggregation or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. Preferably, the modulator compound of the invention is composed of 1-20 D-ammo acids, more preferably 1-10 D-amino acids, even more preferably 1-5 D-amino acids, and most preferably 2-4 D-amino acids which are attached to a hydrazine moiety. In one embodiment, the modulator compounds of the invention which include a hydrazine moiety are amino-terminally modified, for example with a modifying comprising an alkyl group, e.g., a methyl, ethyl, or isopropyl group. Examples of suitable N-terminal modifying groups are described further in subsection II below. In another embodiment, modulator compounds of the invention which include a hydrazine moiety are carboxy-terminally modified, for example with an acetyl. Examples of suitable C-terminal modifying groups are described further in subsections II and III below. The modulator compounds of the invention which include a hydrazine moiety may be modified to enhance the ability of the modulator to alter ß-AP aggregation or neurotoxicity. Additionally or alternatively, the modulator compounds of the invention which include a hydrazine moiety may be modified to alter a pharmacokinetic property of the modulator and/or to label the modulator with a detectable substance (described further in subsection III below). The modulators of the invention preferably are designed based upon the amino acid sequence of a subregion of natural B-AP The term "subregion of a natural 6-amyloid peptide" is intended to include amino-terminal and/or carboxy-terminal deletions of natural ß-AP. The term "subregion of natural ß-AP" is not intended to include full-length natural ß-AP (i.e , "subregion" does not include Aß1-39 Aß1-40, Aß1-41, Aß1-42 and Aß1-43). A preferred subregion of natural ß-amyloid peptide is an "Ap aggregation core domain" (ACD) As used herein, the term "AP aggregation core domain" refers to a subregion of a natural ß-amyloid peptide that is sufficient to modulate aggregation of natural P-APs when this subregion, in its L-amino acid form, is appropriately modified (e.g., modified at the amino-terminus), as described in detail in U.S. patent application Serial No. 08/548,998 and U.S. patent application Serial No 08/616,081, the entire contents of each of which are expressly incorporated herein by reference. Preferably, the ACD is modeled after a subregion of natural ß-AP that is less than 15 amino acids in length and more preferably is between 3-10 amino acids in length. In various embodiments, the ACD is modeled after a subregion of ß-AP mat is 10, 9, 8, 7, 6, 5, 4 or 3 amino acids in length. In one embodiment, the subregion of ß-AP upon which the ACD is modeled is an internal or carboxy-terminal region of ß-AP (i.e , downstream of the amino-terminus at ammo acid position 1). In another embodiment, the ACD is modeled after a subregion of ß-AP that is hydrophobic. Preferred Aß aggregation core domains encompass amino acid residues 17-20 or 17-21 of natural ß-AP (Aß 17.20 andi Aß 17.21, respectively) and analogues thereof, as described herein. The amino acid sequences of Aß17-20 and Aß 17.21 are Leu-Val-Phe-Phe (SEQ ID NO:3) and Leu-Val-Phe-Phe-Ala (SEQ ID NO:4), respectively. As demonstrated in the Examples, D-amino acid-containing modulators designed based upon the amino acid sequences of Aß17-20 and Aß 17.21 are particularly effective inhibitors of Aß aggregation and exhibit an enhanced biostabihty and prolonged elevated plasma levels. These modulators can comprise a D-amino acid sequence corresponding to the L-amino acid sequence of Aß 17.20 or Aß 17.21, a D-amino acid sequence which is an inverso isomer of the L-armno acid sequence of Aß 17.20 or Aß1- 7. 21, a D-amino acid sequence which is a retro-inverso isomer of the L-amino acid sequence of Aß 17.20 or Aß17.2], or a D-amino acid sequence that is a scrambled or substituted version of the L-amino acid sequence of Aß 17-20 or Aß 17-21- In preferred embodiments, a phenylalanine in the modulators designed based upon the amino acid sequences of Aß 17.20 and Aß17-2 is substituted with a phenylalanine analogue which is more stable and less prone to, for example, oxidative metabolism. In other preferred embodiments, the modulators designed based upon the amino acid sequences of Aß17.20 and Aß7.2 further comprise a hydrazine moiety. The D-amino acid-based modulators may have unmodified amino- and/or carboxy-termini and/or carboxy amide termini, or, alternatively, the amino-terminus, the carboxy-terminus, or both, may be modified (described further below). The peptidic structures of effective modulators generally are hydrophobic and are characterized by the presence of at least two D-amino acid structures independently selected from the group consisting of a D-leucine structure, a D-phenylalanine structure and a D-valine structure As used herein, the term a "D-amino acid structure" (such as a "D-leucine structure", a "D-phenylalanine structure" or a "D-valine structure") is intended to include the D-amino acid, as well as analogues, derivatives and mimetics of the D-ammo acid that maintain the functional activity of the compound (discussed further below) For example, the term "D-phenylalanine structure" is intended to include D-phenylalanine as well as D-cyclohexylalanine [D-cha], D-4-fluorophenylalanine (para-fluorophenylalanine) {[ß-F]f or D-[p-F]Phe}, D-pentafluorophenylalanine {[F5]f or D-[F5]Phe}, chlorophenylalanine, bromophenylalanine, nitrophenylalanine, D-pyridylalanine, D-homophenylalanine, methyltyrosine, and benzylserine, as well as substitution with D-lysine structure, D-valine structure, or a D-leucine structure. The term "D-leucine structure" is intended to include D-leucine, as well as substitution with D-valine, D-isoleucine, or other natural or non-natural amino acids having an aliphatic side chain, such as D-norleucine, or D-norvaline The term "D-valine structure" is intended to include D-valine, as well as substitution with D-leucine or other natural or non-natural amino acid having an aliphatic side chain. In other embodiments, the peptidic structure of the modulator comprises at least two D-amino acid structures independently selected from the group consisting of a D-leucine structure, a D-phenylalanine structure, a D-valine structure, a D-alanine structure, a D-tyrosine structure, a D-iodotyrosine structure, and a D-Iysine structure. In another embodiment, the peptidic structure is comprised of at least three D-amino acid structures independently selected from the group consisting of a D-leucine structure, a D-phenylalanine structure and a D-vahne structure. In yet another embodiment, the peptidic structure is comprised of at least three D-amino acid structures independently selected from the group consisting of a D-leucine structure, a D-phenylalanine structure, a D-valine structure, a D-alanine structure, a D-tyrosine structure, a D-iodotyrosine structure, and a D-lysine structure. In yet another embodiment, the peptidic structure comprises at least four D-amino acid structures independently selected from the group consisting of a D-leucine structure, a D-phenylalanine structure and a D-valine structure. In yet another embodiment, the peptidic structure is comprised of at least four D-amino acid structures independently selected from the group consisting of a D-leucine structure a D-phenylalanine structure and a D-valine structure In preferred embodiments, the peptidic structure includes at least one phenylalanine analogue which is more stable than phenylalanine and less prone to, for example, oxidative metabolism. In one embodiment, the invention provides a ß-amyloid modulator compound comprising a formula (I): (Formula Removed) wherein Xaaj, Xaa2, Xaa3 and Xaa4 are each D-amino acid structures and at least two of Xaaj, Xaa2, Xaa3 and Xaa4 are, independently, selected from the group consisting of a D-leucine structure, a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fIuorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalamne, bromophenylalanine, nitrophenyialanine, and D-homophenylalanine, and a D-valine structure; Y, which may or may not be present, is a structure having the formula (Xaa)a, wherein Xaa is any D-amino acid structure and a is an integer from 1 to 15; Z, which may or may not be present, is a structure having the formula (Xaa)b, wherein Xaa is any D-amino acid structure and b is an integer from 1 to 15, A, which may or may not be present, is a modifying group attached directly or indirectly to the compound; and n is an integer from 1 to 15; wherein Xaaj, Xaa2, Xaa3, Xaa4, Y, Z, A and n are selected such that the compound binds to natural ß-amyloid peptides or modulates the aggregation or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural P-amyloid peptides, and is less prone to metabolism, e.g., oxidative metabolism. In a subembodiment of this formula, a fifth amino acid residue, Xaa5, is specified C-terminal to Xaa4 and Z, which may or may not be present, is a structure having the formula. (Xaa)b, whereirrXaa is any D amino acid structure and b is an integer from 1 to 14. Accordingly, the invention provides a ß-amyloid modulator compound comprising a formula (II): (Formula Removed) wherein b is an integer from 1 to 14. In a preferred embodiment, Xaa1, Xaa2, Xaa3, Xaa4 of formula (I) are selected based on the sequence of Aß17.20. or acceptable substitutions thereof Accordingly, in preferred embodiments, Xaa1 is a D-alanine structure or a D-leucine structure, Xaa2 is a D-valine structure or a D-phenylalanine structure, Xaa3 is a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fiuorophenylalanine), D-pentafluorophenylalanine, chlorophenylalamne, bromophenylalanine, nitrophenyialanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure and Xaa4 is a D-phenylalanine structure, e g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosme structure, or a D-lysine structure. In another preferred embodiment, Xaaj, Xaa2, Xaa3, Xaa4 and Xaa5 of formula (II) are selected based on the sequence of Aß]7_21, or acceptable substitutions thereof Accordingly, in preferred embodiments, Xaa1 is a D-alanine structure or a D-leucine structure, Xaa2 is a D-valine structure, Xaa3 is a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure, Xaa4 is a D-phenylalanine structure, e g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine). D-pentafluorophenylalanine, chlorophenylalamne, bromophenylalanine, nitrophenylaianine, D-pyridylalamnerand D homophenylalanine a D-tyrosine structure a D-iodotyrosine structure, or a D-lysine structure, and Xaa5 is a D-alanine structure or a D-leucine structure. In another preferred embodiment, Xaa1. Xaa2, Xaa3 and Xaa4 of formula (I) are selected based on the retro-inverso isomer of Aß]7.20, or acceptable substitutions thereof. Accordingly, in preferred embodiments, Xaaj is a D-alanine structure, a D-leucine structure, or a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fiuorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, a D-leucine structure, a D-valine structure, or a D-lysine structure; Xaa2 is a D-phenylalanine structure, e.g., D-cyclohexylalamne, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenylalanine, D-pyridylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure; Xaa3 is a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenyialanine, D-pyridylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure; and Xaa4 is a D-valine structure or a D-leucine structure. In another preferred embodiment, Xaa1, Xaa2, Xaa3, Xaa4 and Xaa5 of formula (II) are selected based on the retroinverso isomer of Aß 17.21, or acceptable substitutions thereof. Accordingly, in preferred embodiments, Xaaj is a D-alanine structure, a D-leucine structure or a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenyialanine, D-pyridylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure; Xaa2 is a D-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenyialanine, D-pyridylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure; Xaa3 is a D-tryrosine structure eg D cyclohexylalanine,. D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenyialanine, D-pyridylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, or a D-lysine structure; Xaa4 is a D-valine structure or a D-leucine structure and Xaa5 is a D-leucine structure. In another embodiment, the invention provides a ß-amyloid modulator compound comprising a formula (III) (Formula Removed) wherein Xaa1 and Xaa2 are each D-amino acid structures and at least two of Xaa1 and Xaa2 are, independently, selected from the group consisting of a D-leucine structure, a D-phenylalanine structure, e g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitrophenylalanine, and D-homophenylalanine, a D-tyrosine structure, a D-iodotyrosine structure, a D-lysine structure, or a D-valine structure; NH-NH is a hydrazine structure; Y, which may or may not be present, is a structure having the formula (Xaa)a, wherein Xaa is any D-amino acid structure and a is an integer from 1 to 15; Xaaj', Xaa2', and Xaa3' which may or may not be present, are each D-amino acid or L-amino acid structures and at least two of Xaaj', Xaa2\ and Xaa3*are, independently, selected from the group consisting of a D- or L-leucine structure, a D- or L-phenylalanine structure, e.g., D-cyclohexylalanine, D-4-fluorophenylalanine (para-fluorophenylalanine), D-pentafluorophenylalanine, chlorophenylalanine, bromophenylalanine, nitropheriylalanine, and D-homophenylalanine, a D- or L-tyrosme structure, a D- or L-iodotyrosine structure, a D- or L-lysme structure, or a D- or L-valine structure; Z, which may or may not be present, is a structure having the formula (Xaa)^ wherein X aa is any D amion acid struture and b is an integer from 1 to 15: A, which may or may not be present, is a modifying group attached directly or indirectly to the compound; and n is an integer from 1 to 15; wherein Xaaj, Xaa2, Xaaj', Xaa2', Xaa3', Y, Z, A and n are selected such that the compound binds to natural ß-amyloid peptides or modulates the aggregation or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides, and is less prone to metabolism, e.g., oxidative metabolism. In the modulators of the invention having the formula (I), (II), or (III) shown above, an optional modifying group ("A") is attached directly or indirectly to the peptidic structure of the modulator. (As used herein, the term "modulating group" and "modifying group" are used interchangeably to describe a chemical group directly or indirectly attached to a peptidic structure). For example, a modifying group(s) can be directly attached by covalent coupling to the peptidic structure or a modifying group(s) can be attached indirectly by a stable non-covalent association. In one embodiment of the invention, a modifying group is attached to the amino-terminus of the modulator Alternatively, in another embodiment of the invention, a modifying group is attached to the carboxy-terminus of the modulator. In other embodiments, the modifying group is attached to both the amino and the carboxy-terminus of the modulator. In yet another embodiment, a modulating group(s) is attached to the side chain of at least one amino acid residues of the peptidic structure of the modulator (e g., through the epsilon amino group of a Iysyl residue(s), through the carboxyl group of an aspartic acid residue(s) or a glutamic acid residue(s), through a hydroxy group of a tyrosyl residue(s), a serine residue(s) or a threonine residue(s) or other suitable reactive group on an amino acid side chain). If a modifying group(s) is present, the modifying group is selected such that the compound inhibits aggregation of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. Accordingly, since the ß-AP peptide of the compound is modified from its natural state, the modifying group "A" as used herein is not intended to include hydrogen. In a modulator of the invention, a single modifying group may be atached to the peptidic structure or multiple modifying group may be attached to the peptidic structure. The number of modifying groups is selected such that the compound inhibits aggregation of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. However, n preferably is an integer between 1 and 60, more preferably between 1 and 30 and even more preferably between 1 and 10 or 1 and 5. In a preferred embodiment, A is an amino-terminal modifying group comprising a cyclic, heterocyclic, polycyclic, linear, or branched alkyl group and n=l. In another preferred embodiment, A is carboxy-terminally modifying group comprising an amide group, an alkyl amide group, an aryl amide group or a hydroxy group, and n=l. Suitable modifying groups are described further in subsections II and III below. In preferred specific embodiments, the invention provides a ß-amyloid modulator compound comprising a peptidic structure selected from the group consisting of (D-Leu-D-Val-D-Phe-D-Cha-D-Leu) (SEQ ID NO:5); (D-Leu-D-Val-D-Cha-D-Phe-D-Leu) (SEQ ID NO:6); (D-Leu-D-Val-D-Phe-D-[ß-F]Phe-D-Leu) (SEQ ID NO:7); (D-Leu-D-Val-D-fc-F]Phe-D-Phe-D-Leu) (SEQ ID N08); (D-Leu-D-Val-D-Phe-D-[F- 5]Phe-D-Leu) (SEQ ID N0:9); (D-Leu-D-Val-D-[F5]Phe-D-Phe-D-Leu) (SEQ ID NO: 10); (D-Leu-D-Phe-D-Cha-D-Val-D-Leu) (SEQ ID NO 11); (D-Leu-D-Phe-D-[ß-F]Phe-D-Val-D-Leu) (SEQ ID NO 12); D-Leu-D-Phe-D-[F5]Phe-D-Val-D-Leu) (SEQ ID NO: 13); (D-Leu-D-Phe-D-Lys-D-Val-D-Leu) (SEQ ID NO: 14); (D-Leu-D-Cha-D-Phe-D-Val-D-Leu) (SEQ ID NO: 15); (D-Leu-D-[ß-F]Phe-D-Phe-D-Val-D-Leu) (SEQ ID NO: 16); (D-Leu-D-[F5]Phe-D-Phe-D-Val-D-Leu) (SEQ ID NO: 17); (D-Leu-D-Lys-D-Phe-D-Val-D-Leu) (SEQ ID NO" 18); (D-Leu-D-Cha-D-Cha-D-Val-D-Leu) (SEQ ID NO: 19); (D-Leu-D-Val-D-Cha-D-Cha-D-Leu) (SEQ ID NO:20); (D-Leu-D-[ß-F]Phe-D-[ß-F]Phe-D-Val-D-Leu) (SEQ ID NO 21), (D-Leu-D-Val-D-[ß-F]Phe-D-|>-F]Phe-D-Leu) (SEQ ID NO:22); (D-Leu-D-[F5]Phe-D-[F5]Phe-D-Val-D-Leu) (SEQ ID NO:23); (D-Leu-D-Val-D-[F5]Phe-D-[F5]Phe-D-Leu) (SEQ ID NO:24); (D-Leu-D-Val-D-Phe) (SEQDNO:25). Any of the aforementioned specific peptidic structures can be amino-terminally and/or carboxy-terminally modified and described further in subsections II and/or III below. Particularly preferred modulators of the invention include the following N,N-dimethyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N,N-dimethyl-(D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-methyl-(GIy-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-ethyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-isopropyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;H-(D-Leu-D-Val-D-Phe-D-Phe-D-Ala)-isopropylamide;H-(D-Leu-D-Val-D-Phe-D-Phe-D-Ala)-dimethylamide;N,N-diethyl-(Gly-D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2,N,N-diethyl-(D-Ala-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N^-dimethyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N,N-dimethyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N,N-dimethyl-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH2;H-(Gly-D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N-ethyl-(Gly-D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;N-ethyl-(Gly-D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-methyl-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH2;N-ethyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2)N-propyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2; N,N-diethyl-(Gly-D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;H-(D-Ile-D-Val-D-Phe-D-Phe-D-Ile)-NH2; H-(D-Ile-D-Val-D-Phe-D-Phe-D-Ala-)-NH2; H-( D-Ile- D-Ile-D-Phe-D-Phe- D-Ile)-NH2; H-(D-Nle-D-Val-D-Phe-D-Phe-D-Ala-)-NH2; H-(D-Nle-D-Val-D- Phe-D-Phe-D-Nle>NH2, l-piperidme-acetyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2, l-piperidine-acetyl-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-NH2;H-D-Leu-D-Val-D-Phe-D-Phe-D-Leu-isopropylamide; H-D-Leu-D-Phe-D-Phe-D-Val-D-Leu-isopropylamide, H-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-methylamide;H-(D-Leu-D-Phe-D-Phe-D-Val-D-Leu)-methylamide;H-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-OH;N-methyI-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2;H-(D-Leu-D-Val-D-Phe-D-Cha-D-Leu)-NH2, H-(D-Leu-D-VaI-D-Phe-D-[ß-F]Phe-D-Leu)-NH2;H-(D-Leu-D-Val-D-Phe-D-[F5]Phe-D-Leu)-NH2; H-(D-Leu-D-Phe-D-Cha-D-Val-D-Leu)-NH2; H-(D-Leu-D-Phe- D-[ß-F]Phe-D-Val-D-Leu)-NH2; H-(D-Leu-D-Phe- D-[F5]Phe-D-Val-D-Leu)-NH2; H-(D-Leu-D-Phe-D-Lys-D-Val-D-Leu)-NH2;H-(D-Leu-D-Cha-D-Phe-D-Val-D-Leu)-NH2;H-(D-Leu-D-{/7-F3Phe-D-Phe-D-VaI-D-Leu)-NH2;H-(D-Leu-D-[F5]Phe-D-Phe-D-Val-D-Leu)-NH2; H-(D-Leu- D-Lys-D-Phe-D-Val-D-Leu)-NH2; H-(D-Leu-D-Cha-D-Cha-D-Val-D-Leu)-NH2; H-(D-Leu- D-(ß-F]Phe-D-[ß-F]Phe-D-Val-D-Leu)-NH2, H-(D-Leu-D-[F5]Phe-D-[F5]Phe-D-Val-D-Leu)-NH2; H-(D-Leu- D-Lys- D-Lys-D-Val-D-Leu)-NH2, N-raethyl-(D-Leu-D-Val-D-Phe-D-Cha-D-Leu)-NH2;N-methyl-(D-Leu-D-Val-D-Phe-D fe F Leu ffl2+JsI.4aetliyl D4Leu- D-Lys- D-Lys-D-Val-D-Leu)-NH2, D-Leu-D-Val-D-Phe-NH-(H-D-Leu-D-Val-D-Phe-)NH;H-D-Leu-D-Val-D-Phe-NH-NH-COCH3; and H- D-Leu-D-Val-D-Phe-NH-NH,. Even more preferred compounds of the invention include PPI-1319: H-(D-Leu-D-Phe-[>-F]D-Phe-D-Val-D-Leu)-NH2 and PPI: 1019: N-methyl-(D-Leu-D-Val-D-Phe-D-Phe-D-Leu)-NH2. (As described above, D-Cha stands for D-cyclohexylalamne; [p-F]f or D-[ß-F]Phe stands for D-4-fiuorophenylalanine (also pcrra-fluorophenylalanine); [F5]f or D-[ F5]Phe stands for D-pentafluorophenylalamne; and D-Nle stands for D-norleucine). The D-amino acid peptidic structures of the modulators of the invention are further intended to include other peptide modifications, including analogues, derivatives and mimetics, that retain the ability of the modulator to alter natural ß-AP aggregation as described herein. For example, a D-amino acid peptidic structure of a modulator of the invention may be further modified to increase its stability, bioavailability, and solubility The terms "analogue", "derivative" and "mimetic" as used herein are intended to include molecules which mimic the chemical structure of a D-peptidic structure and retain the functional properties of the D-peptidic structure. Approaches to designing peptide analogs, derivatives and mimetics are known in the art. For example, see Farmer, P.S. in Drug Design (EJ. Ariens, ed.) Academic Press, New York, 1980, vol. 10, pp. 119-143; Ball. J.B. and Alewood, P.F. (1990) J Mol Recognition 3:55; Morgan, B.A. and Gainor, J.A. (1989) Ann Rep Med Chem. 24:243; and Freidinger, R.M. (1989) Trends Pharmacol. Sci. H):270. See also Sawyer, T.K. (1995) "Peptidomimetic Design and Chemical Approaches to Peptide Metabolism" in Taylor, M.D. and Amidon, G.L. (eds.) Peptide-Based Drug Design: Controlling Transport and Metabolism, Chapter 17; Smith, A.B. 3rd, et al. (1995) J. Am. Chem. Soc. 117:11113-11123; Smith, A.B. 3rd, et al (1994) J. Am Chem Soc. 116:9947-9962; and Hirschman, K,etal (1993) J Am. Chem Soc 115:12550-12568. As used herein, a "derivative" of a compound X(e g,a peptide or amino acid) refers to a form of X in which one or more reaction groups on the compound have been derivatized with a substituent group. Examples of peptide derivatives include peptides in which an amino acid side chain, the peptide backbone, or the amino- or carboxy- terminus has been derivatized (eg peptidic compounds with methylated amide_ linkages). As used herein an "analogue" of a compound X refers to a compound which retains chemical structures of X necessary for functional activity of X yet which also contains certain chemical structures which differ from X. An examples of an analogue of a naturally-occurring peptide is a peptide which includes one or more non-naturally-occurring amino acids. As used herein, a "mimetic" of a compound X refers to a compound in which chemical structures of X necessary for functional activity of X have been replaced with other chemical structures which mimic the conformation of X. Examples of peptidomimetics include peptidic compounds in which the peptide backbone is substituted with one or more benzodiazepine molecules (see e.g., James, G.L. etal. (1993) Science 260:1937-1942). Analogues of the modulator compounds of the invention are intended to include compounds in which one or more D-amino acids of the peptidic structure are substituted with a homologous amino acid such that the properties of the original modulator are maintained. Preferably conservative amino acid substitutions are made at one or more amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art, including basic side chains (e g, lysine, arginine, histidine), acidic side chains (e g, aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g, alanine, valine, leucine, isoleucine, prohne, phenylalanine, methionine, tryptophan), P-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e g., tyrosine, phenylalanine, tryptophan, histidine). Non-limiting examples of homologous substitutions that can be made in the peptidic structures of the modulators of the invention include substitution of D-phenylalanine with D-tyrosine, D-pyridylalanine or D-homophenylalanine, substitution of D-leucine with D-valine or other natural or non-natural amino acid having an aliphatic side chain and/or substitution of D-valine with D-leucine or other natural or non-natural amino acid having an aliphatic side chain. The term mimetic, and in particular, peptidomimetic, is intended to include isosteres. The term "isostere" as used herein is intended to include a chemical structure that can be substituted for a second chemical structure because the steric conformation of the first structure fits a binding site specific for the second structure. The term specifically includes peptide back-bone modifications (i.e., amide bond mimetics) well known to those skilled in the art. Such modifications include modifications of the amide nitrogen, the ct-carbon, amide carbonyl, complete replacement of the amide bond, extensions, deletions or backbone crosslinks. Several peptide backbone modifications are known, including [CH2S], [GH2NH], [CSNH2],[NHCO],[COCH2], and [(E) or (Z) CH=CH]. In the nomenclature used above,  indicates the absence of an amide bond. The structure that replaces the amide group is specified within the brackets. Other possible modifications include an N-alkyl (or aryl) substitution ([CONR]), or backbone crosslinking to construct lactams and other cyclic structures. Other derivatives of the modulator compounds of the invention include C-terminal hydroxymethyl derivatives, O-modified derivatives (e g, C-terminal hydroxymethyl benzyl ether), N-terminally modified derivatives including substituted amides such as alkylamides and hydrazides and compounds in which a C-terminal phenylalanine residue is replaced with a phenethylamide analogue (e.g, Val-Phe-phenethylamide as an analogue of the tripeptide Val-Phe-Phe) The modulator compounds of the invention can be incorporated into pharmaceutical compositions (described farther in subsection V below) and can be used in detection and treatment methods as descnbed further in subsection VI below. II. Modifying Groups In certain embodiments, the modulator compounds of the invention are coupled directly or indirectly to at least one modifying group (abbreviated as MG). The term "modifying group" is intended to include structures that are directly attached to the D-amino acid peptidic structure (e.g., by covalent coupling), as well as those that are indirectly attached to the peptidic structure (e g, by a stable non-covalent association or by covalent coupling to additional amino acid residues, or mimetics, analogues or derivatives thereof, which may flank the Aß-denved D-amino acid peptidic structure) For example the modifying group can be coupled to the amino terminus or caboxy terminus of an Aß-derived D-amino acid peptidic structure, or to a peptidic or peptidomimetic region flanking the core domain. Alternatively, the modifying group can be coupled to a side chain of at least one D-amino acid residue of an AP-derived D-amino acid peptidic structure, or to a peptidic or peptidomimetic region flanking the core domain (e.g, through the epsilon ammo group of a lysyl residue(s), through the carboxyl group of an aspartic acid residue(s) or a glutamic acid residue(s), through a hydroxy group of a tyrosyl residue(s), a serine residue(s) or a threonine residue(s) or other suitable reactive group on an amino acid side chain). Modifying groups covalently coupled to the D-amino acid peptidic structure can be attached by means and using methods well known in the art for linking chemical structures, including, for example, amide, alkylamino, carbamate, urea or ester bonds. The term "modifying group" is intended to include groups that are not naturally coupled to natural Aß peptides in their native form. Accordingly, the term "modifying group" is not intended to include hydrogen The modifying group(s) is selected such that the modulator compound alters, and preferably inhibits, aggregation of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides or inhibits the neurotoxicity of natural ß-amyloid peptides when contacted with the natural ß-amyloid peptides. Although not intending to be limited by mechanism, in embodiments where the modulator comprises a modifying group(s), the modifying group(s) is thought to function as a key pharmacophore that enhances the ability of the modulator to disrupt Aß polymerization. In a preferred embodiment, the modifying group(s) comprises an alkyl group. The term "alkyl", as used herein, refers to a straight or branched chain hydrocarbon group having from about 1 to about 10 carbon atoms. Exemplary alkyl groups include methyl, ethyl, dimethyl, diethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, and n-hexyl. An alkyl group may be unsubstituted, or may be substituted at one or more positions, with, e.g., halogens, alkyls, cycloalkyls, alkenyls, alkynyls, aryls, heterocycles, hydroxyls. aminos, nitros, thiols, amines, imines, amides, phosphonates, phosphines, carbonyls, carboxyls, silyls, ethers, thioethers, sulfonyls, selenoethers, ketones, aldehydes, esters, -CF3, -CN, or the like. Preferred" alkyls are methyls, ethyls, dimethyls, diethyls, n-propyls, isopropyls. In another embodiment, one modifying group, e.g., an alkyl group, is coupled to another modifying group. In yet another embodiment, a D-amino acid in a modulator compound of the invention is modified with two modifying groups. Accordingly, preferred modifying groups include a 1-piperidine acetyl group. In a preferred embodiment, the modifying group(s) comprises a cyclic, heterocyclic, polycyclic or branched alkyl group. The term "cyclic group", as used herein, is intended to include cyclic saturated or unsaturated (i.e., aromatic) group having from about 3 to 10, preferably about 4 to 8, and more preferably about 5 to 7, carbon atoms. Exemplary cyclic groups include cyclopropyl, cyclobutyl, cyclopentyl. cyclohexyl, and cyclooctyl. Cyclic groups may be unsubstituted or substituted at one or more ring positions. Thus, a cyclic group may be substituted with, e g, halogens, alkyls, cycloalkyls, alkenyls, alkynyls, aryls, heterocycles, hydroxyls, aminos, nitros, thiols amines, imines, amides, phosphonates, phosphines, carbonyls, carboxyls, silyls, ethers, thioethers, sulfonyls, sulfonates, selenoethers, ketones, aldehydes, esters, -CF3, -CN, or the like. The term "heterocyclic group" is intended to include cyclic saturated or unsaturated {i.e , aromatic) group having from about 3 to 10, preferably about 4 to 8, and more preferably about 5 to 7, carbon atoms, wherein the ring structure includes about one to four heteroatoms. Heterocyclic groups include pyrrolidine, oxolane, thiolane, imidazole, oxazole, piperidine, piperazine, morpholine and pyridine. The heterocyclic ring can be substituted at one or more positions with such substituents as, for example, halogens, alkyls, cycloalkyls, alkenyls, alkynyls, aryls, other heterocycles, hydroxyl, amino, nitro, thiol, amines, imines, amides, phosphonates, phosphines, carbonyls, carboxyls, silyls, ethers, thioethers, sulfonyls, selenoethers, ketones, aldehydes, esters, -CF3, -CN, or the like. Heterocycles may also be bridged or fused to other cyclic groups as described below. The term "polycyclic group" as used herein is intended to refer to two or more saturated or unsaturated (/ e., aromatic) cyclic rings in which two or more carbons are common to two adjoingn rings eg. the rings are fused rings". Rings that are joined through non-adjacent atoms are termed "bridged" rings. Each of the rings of the polycyclic group can be substituted with such substituents as described above, as for example, halogens, alkyls, cycloalkyls, alkenyls, alkynyls, hydroxyl, amino, nitro, thiol, / amines, imines, amides, phosphonates, phosphines, carbonyls, carboxyls, silyls, ethers, thioethers, sulfonyls, selenoethers, ketones, aldehydes, esters, -CF3, -CN, or the like. A preferred polycyclic group is a group containing a cis-decalin structure. Although not intending to be limited by mechanism, it is thought that the "bent" conformation conferred on a modifying group by the presence of a cis-decalin structure contributes to the efficacy of the modifying group in disrupting Aß polymerization Accordingly, other structures which mimic the "bent" configuration of the cis-decalin structure can also be used as modifying groups. An example of a cis-decalin containing structure that can be used as a modifying group is a cholanoyl structure, such as a cholyl group. For example, a modulator compound can be modified at its amino terminus with a cholyl group by reacting the aggregation core domain with cholic acid, a bile acid. Moreover, a modulator compound can be modified at its carboxy terminus with a cholyl group according to methods known in the art (see e g, Wess, G. et al (1993) Tetrahedron Letters, 34:817-822; Wess, G. et al (1992) Tetrahedron Letters 33:195-198; and Kramer, W. et al. (1992) J. Biol. Chem. 267:18598-18604) Cholyl derivatives and analogues can also be used as modifying groups. For example, a preferred cholyl derivative is Aic (3-((O-aminoethyl-iso )lyl), which has a free amino group that can be used to further modify the modulator compound {e.g., a chelation group for 99mTc can be introduced through the free amino group of Aic). As used herein, the term "cholanoyl structure" is intended to include the cholyl group and derivatives and analogues thereof, in particular those which retain a four-ring cis-decalin configuration Examples of cholanoyl structures include groups derived from other bile acids, such as deoxycholic acid, lithocholic acid, ursodeoxycholic acid, chenodeoxycholic acid and hyodeoxycholic acid, as well as other related structures such as cholanic acid, bufalin and resibufogenin (although the latter two compounds are not preferred for use as a modifying group). Another example of a cis-decalin containing compound is 5ß-cho!estan-3a-ol (the cis-decalin isomer of (+)-dihydrocholesterol). For further description or bile acid and steroid-structure-and nomeclature see Nes , W R, and, McKean, M.L. Biochemistry of Steroids and Other Isopentanoids, University Park Press, Baltimore, MD, Chapter 2. In addition to cis-decalin containing groups, other polycyclic groups may be used as modifying groups. For example, modifying groups derived from steroids or (3-lactams may be suitable modifying groups. In one embodiment, the modifying group is a "biotinyl structure", which includes biotinyl groups and analogues and derivatives thereof (such as a 2-iminobiotinyl group). In another embodiment, the modifying group can comprise a "fluorescein-containing group", such as a group derived from reacting an Aß-derived peptidic structure with 5-(and 6-)-carboxyfluorescein, succmimidyl ester or fluorescein isothiocyanate. In various other embodiments, the modifying group(s) can comprise an Ts'-acetylneuraminyl group, a trans-4-cotininecarboxyl group, a 2-imino-3-imidazolidineacetyl group, an (S)-(-)-indoline-2-carboxyl group, a (-)-menthoxyacetyl group, a 2-norbornaneacetyl group, a -oxo-5-acenaphthenebutyryl, a (-)-2-oxo-4-thiazolidinecarboxyl group, atetrahydro-3-furoyl group, a 2-iminobiotinyl group, adiethylenetriaminepentaacetyl group, a 4-morpholinecarbonyl group, a 2-thiopheneacetyl group or a 2-thiophenesulfonyl group In addition to the cyclic, heterocyclic and polycyclic groups discussed above, other types of modifying groups can be used in a modulator of the invention. For example, hydrophobic groups and branched alkyl groups may be suitable modifying groups. Examples include acetyl groups, phenylacetyl groups, phenylacetyl groups, diphenylacetyl groups, triphenylacetyl groups, isobutanoyi groups, 4-methylvaleryl groups,trans-cinnamoyl groups, butanoyl groups and 1-adamantanecarbonyl groups. Yet another type of modifying group is a compound that contains a non-natural amino acid that acts as a beta-turn mimetic, such as a dibenzofuran-based amino acid described in Tsang, K.Y. et al. (1994) J. Am Chem Soc. U6:398S-4005; Diaz, H and Kelly, J.W. (1991) Tetrahedron Letters 41:5725-5728, and Diaz. H et al. (1992) J Am Chem. Soc. 114:8316-8318. An example of such a modifying group is a peptide-ammoethyldibenzofuranyl-proprionic acid (Adp) group (e.g., DDIIL-Adp) (SEQ ID NO 31). This type of modifying group further can comprise one or more N-methyl peptide bonds to introduce additional steric hindrance lo the aggregation of naturaJ-ft-AP-^when- — compounds of this type interact with natural ß-AP Yet another type of modifying group is an NH-OR group, where the R can be any of the modified or umodified alkyl or cycloalkyl groups described herein. Non-limiting examples of suitable modifying groups, with their corresponding modifying reagents, are listed below. Modifying Group Modifying Reagent Methyl- Methylamine, Fmoc-D-[Me]-Leu- OH,methylannne and a bromoacetylpeptide Ethyl- Ethylamine, acetaldehyde and sodium cyanoborohydride, ethylamine and a bromoacetylpeptide Propyl- Propylamine, propionaldehyde and sodium cyanoborohydride, propylamine and a bromoacetylpeptide Isopropyl- Isopropylamine, isopropylamine and a bromoacetylpeptide Piperidine- Piperidine and a bromoacetylpeptide Acetyl- Alette anhydride, arctic arid Dimethyl- Methylamine, formaldehyde and sodium cyanoborohydride Diethyl- Acetaldehyde and sodium cyanoborohydride Cholyl- Chohc acid Lithocholyl- Lithochohc acid Hyodeoxycholyl- Hyodeoxycholic acid Chenodeoxycholyl- Chenodeoxycholic acid Ursodeoxycholyl- Ursodeoxycholic acid 3-Hydroxycinnamoyl- 3-Hydroxycinnamic acid 4-Hydroxycinnamoyl- 4-Hydroxycinnamic acid 2-Hydroxycinnamoyl- 2-Hydroxycinnamic acid 3-Hydroxy-4-methoxycinnamoyl- 3-Hydroxy-4-methoxycinnamic acid 4-Hydroxy-3-methoxycinnamoyl- 4-Hydroxy-3-methoxycinnamic acid 2-Carboxycinnamoyl- 2-Carboxycinnamic acid 3-Formylbenzoyl 3-Carboxybenzaldehyde 4-FormyIbenzoyl 4-Carboxybenzaldehyde 3,4,-Dihydroxyhydrocinnamoyl- 3,4,-Dihydroxyhydrocinnamic acid 3,7-Dihydroxy-2-napthoyl- 3,7-Dihydroxy-2-naphthoic acid 4-Formylcinnamoyl- 4-Formylcinnamic acid 2-Formylphenoxyacetyl- 2-Formylphenoxyacetic acid 8-Formyl-1 -napthoyl 1,8-napthaIdehydic acid 4-(hydroxymethyl)benzoyl- 4-(hydroxymethyI)benzoic acid 4-Hydroxyphenylacetyl- 4-Hydroxyphenylacetic acid 3-Hydroxybenzoyl- 3-Hydroxybenzoic acid 4-HydroxybenzoyI- 4-Hydroxybenzoic acid 5-Hydantoinacetyl- 5-Hydantoinacetic acid L-Hydroorotyl- L-Hydroorotic acid 4-Methylvaleryl- 4-Methylvaleric acid 2,4-Dihydroxybenzoyl- 2,4-Dihydroxybenzoic acid ~3,4-Dihy dtoxycinoaffieyl— 3,4-Oihyriroxycipnamic acid 3,5-Dihydroxy-2-naphthoyl- 3,5-Dihydroxy-2-naphthoic acid 3-BenzoyIpropanoyl- 3-Benzoylpropanoic acid trans-Cmnamoyl- trans-Cmnamic acid Phenylacetyl- Phenylacetic acid Diphenylacetyl- Diphenylacetic acid Triphenylacetyl- Triphenylacetic acid 2-Hydroxyphenylacetyl- 2-Hydroxyphenylacetic acid 3-Hydroxyphenylacetyl- 3-Hydroxyphenylacetic acid 4-Hydroxyphenyiacetyl- 4-Hydroxyphenylacetic acid (±)-Mandelyl- (±)-Mandelic acid (±)-2,4-Dihydroxy-3,3-dimethylbutanoyl (±)-Pantolactone Butanoyl- Butanoic anhydride Isobutanoyl- Isobutanoic anhydride Hexanoyl- Hexanoic anhydride Propionyl- Propionic anhydride 3 -Hydroxy butyroyl ß-Butyrolactone 4-Hydroxybutyroyl y-Butyrokctone 3-Hydroxypropionoyl ß-Propiolactone 2,4-Dihydroxybutyroyl α-Hydroxy-ß-Butyrolactone I - Adamantanecarbonyl- 1 - Adamantanecarbonic acid Glycolyl- Glycolic acid DL-3-(4-hydroxyphenyl)lactyl- DL-3-(4-hydroxyphenyl)lactic acid 3-(2-HydroxyphenyJ)propionyl- 3-(2-Hydroxyphenyl)propionic acid 4-(2-HydroxyphenyI)propionyI- 4-(2-HydroxyphenyI)propionic acid D-3-Phenyllactyl-. D-3-Phenyllactic acid Hydrocinnamoyl- Hydrocinnamic acid 3-(4-Hydroxyphenyl)propionyl- 3-(4-Hydroxyphenyl)propionic acid L-3-Phenyllactyl- L-3-Phenyllactic acid 4-methylvaleryl 4-methylvaleric acid 3-pyridylacetyl 3-pyridylacetic acid 4-pyndylacetyl 4-pyridylacetic acid Isonicotinoyl 4-quinolinecarboxyl 4-quinolinecarboxylic acid 1 -isoquinolinecarboxyl 1 -isoquinohnecarboxylic acid 3-isoquinolinecarboxyl 3-isoqumoIinecarboxyiic acid Preferred modifying groups include methyl-containing groups, ethyl-containing groups, propyl-containing groups, and piperidine-containing groups, e.g., a 1-piperidine-acetyl group. Ill Additional Chemical Modifications of Aß Modulators A ß-amyloid modulator compound of the invention can be further modified to alter the specific properties of the compound while retaining the ability of the compound to alter Aß aggregation and inhibit Aß neurotoxicity For example, in one embodiment, the compound is further modified to alter a pharmacokinetic property of the compound, such as in vivo stability or half-life. In another embodiment, the compound is further modified to label the compound with a detectable substance. In yet another embodiment, the compound is further modified to couple the compound to an additional therapeutic moiety Schematically, a modulator of the invention comprising a D-amino acid Aß aggregation core domain coupled directly or indirectly to at least one modifying group can be illustrated as MG-ACD, whereas this compound which has been further modified to alter the properties of .the modulator can be illustrated as MG-A CD-CM, wherein CM represents an additional chemical modification. To further chemically modify the compound, such as to alter the pharmacokinetic properties of the compound, reactive groups can be derivatized. For example, when the modifying group is attached to the ammo-terminal end of the aggregation core domain, the carboxy-terminal end of the compound can be further modified. Preferred C-terminal modifications include those which reduce the ability of the compound to act as a substrate for carboxypeptidases. Examples of preferred C-terminal modifiers include an amide group (ie . a peptide amide"), an alkyl or aryl amide group (e g, an ethylamide group or a phenethylamide group) a hydroxy group (/ e., a peptide alcohol) and various non-natural ammo acids, such as D-amino acids and |3-alanine. Alternatively, when the modifying group is attached to the carboxy-terminal end of the aggregation core domain, the ammo-terminal end of the compound can be further modified, for example, to reduce the ability of the compound to act as a substrate for aminopeptidases. A modulator compound can be further modified to label the compound by reacting the compound with a detectable substance. Suitable detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, P-galactosidase, or acetylcholinesterase, examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; and examples of suitable radioactive material include 14C, 123, 124I 125I 131I 99m-pc S or H. in a preferred embodiment, a modulator compound is radioactively labeled with 14C, either by incorporation of 14C into the modifying group or one or more amino acid structures in the modulator compound. Labeled modulator compounds can be used to assess the in vivo pharmacokinetics of the compounds, as well as to detect Aß aggregation, for example for diagnostic purposes. Aß aggregation can be detected using a labeled modulator compound either in vivo or in an in vitro sample derived from a subject. Preferably, for use as an in vivo diagnostic agent, a modulator compound of the invention is labeled with radioactive technetium or iodine. Accordingly, in one embodiment, the invention provides a modulator compound labeled with technetium, preferably 99mTc. Methods for labeling peptide compounds with technetium are known in the art (see e.g., U.S. Patent Nos. 5,443,815, 5,225,180 and 5,405,597, all by Dean et al; Stepniak-Biniakiewicz, D., et al. (1992) J. Med. Chem. 35:274-279; Fritzberg, A.R., Tr al (1988) Proc. Natl. Acd Scu USA 85 40.25 -4029 bidoo ke eat Cancer Res. Suppl. 50:799s-803s; and Regan, L. and Smith, C.K. (1995) Science 270:980-982). A modifying group can be chosen that provides a site at which a chelation group for 99mTc can be introduced, such as the Aic derivative of cholic acid, which has a free amino group. In another embodiment, the invention provides a modulator compound labeled with radioactive iodine. For example, a phenylalanine residue within the Aß sequence (such as Phe; 9 or Phe^o) can be substituted with radioactive iodotyrosyl. Any of the various isotopes of radioactive iodine can be incorporated to create a diagnostic agent. Preferably, 123I (half-life = 13.2 hours) is used for whole body scintigraphy, 124I (half life = 4 days) is used for positron emission tomography (PET), 125I (half life = 60 days) is used for metabolic turnover studies and 131I (half life = 8 days) is used for whole body counting and delayed low resolution imaging studies. Furthermore, an additional modification of a modulator compound of the invention can serve to confer an additional therapeutic property on the compound. That is, the additional chemical modification can comprise an additional functional moiety For example, a functional moiety which serves to break down or dissolve amyloid plaques can be coupled to the modulator compound. In this form, the MG-ACD portion of the modulator serves to target the compound to Aß peptides and disrupt the polymerization of the Aß peptides, whereas the additional functional moiety serves to break down or dissolve amyloid plaques after the compound has been targeted to these sites. In an alternative chemical modification, a ß-amyloid compound of the invention is prepared in a "prodrug" form, wherein the compound itself does not modulate A|3 aggregation, but rather is capable of being transformed, upon metabolism in vivo, into a ß-amyloid modulator compound as defined herein. For example, in this type of compound, the modulating group can be present in a prodrug form that is capable of being converted upon metabolism into the form of an active modulating group. Such a prodrug form of a modifying group is referred to herein a secondary modifying group." A variety of strategies are known in the art for preparing peptide prodrugs that limit metabolism m order to optimize delivery of the active form of the peptide-based drug (see e.g., Moss, J. (1995) in Peptide-Based Drug Design: Controlling Transport and Metabolism, Taylor, M.D. and Amidon, G.L. (eds), Chapter 18. Additionally strategies have been specifically tailored to achieving CNS delivery based on "sequential metabolism" (see e.g., Bodor, N., et al (1992) Science 257jl 698-1700; Prokai, L., et al. (1994) J Am. Chem. Soc. 116:2643-2644, Bodor, N. and Prokai, L. (1995) in Peptide-Based Drug Design: Controlling Transport and Metabolism, Taylor, M.D. and Amidon. G.L. (eds), Chapter 14. In one embodiment of a prodrug form of a modulator of the invention, the modifying group comprises an alkyl ester to facilitate blood-brain barrier permeability. Modulator compounds of the invention can be prepared by standard techniques known in the art. The peptide component of a modulator can be synthesized using standard techniques such as those described in Bodansky, M. Principles of Peptide Synthesis, Springer Verlag, Berlin (1993) and Grant, G.A (ed.). Synthetic Peptides. A User's Guide, W.H. Freeman and Company, New York (1992). Automated peptide synthesizers are commercially available (e.g , Advanced ChemTech Model 396; Milligen/ Biosearch 9600). Additionally, one or more modulating groups can be attached to the Aß-derived peptidic component (e g , an Aß aggregation core domain) by standard methods, for example using methods for reaction through an amino group (e.g, the alpha-amino group at the amino-terminus of a peptide), a carboxyl group (e g, at the carboxy terminus of a peptide), a hydroxyl group (e.g., on a tyrosine, serine or threonine residue) or other suitable reactive group on an amino acid side chain (see e.g., Greene, T.W and Wuts, P.GM. Protective Groups in Organic Synthesis, John Wiley and Sons, Inc., New York (1991). Exemplary syntheses of D-amino acid ß amyloid modulator are described further in Example 1 IV. Screening Assays Another aspect of the invention pertains to a method for selecting a modulator of ß-amyloid aggregation In the method a test compounds is contacted with natrual ß amyloid peptides, the aggregation of the natural ß-AP is measured and a modulator is selected based on the ability of the test compound to alter the aggregation of the natural ß-AP (e.g, inhibit or promote aggregation). In a preferred embodiment, the test compound is contacted with a molar excess amount of the natural ß-AP. The amount and/or rate of natural ß-AP aggregation in the presence of the test compound can be determined by a suitable assay indicative of ß-AP aggregation, as described herein (see eg, Example 2). In a preferred assay, the natural ß-AP is dissolved in solution in the presence of the test compound and aggregation of the natural ß-AP is assessed in a nucleation assay (see Example 2) by assessing the turbidity of the solution over time, as measured by the apparent absorbance of the solution at 405 nm (described further in Example 2; see also Jarrett et al. (1993) Biochemistry 32 4693-4697). In the absence of a ß-amyloid modulator, the A405m of the solution typically stays relatively constant during a lag time in which the ß-AP remains in solution, but then the A4o5nm of the solution rapidly increases as the ß-AP aggregates and comes out of solution, ultimately reaching a plateau level (/ e , the A405nrn of the solution exhibits sigmoidal kinetics over time). In contrast, in the presence of a test compound that inhibits ß-AP aggregation, the A405njm of the solution is reduced compared to when the modulator is absent. Thus, in the presence of the inhibitory modulator, the solution may exhibit an increased lag time, a decreased slope of aggregation and/or a lower plateau level compared to when the modulator is absent. This method for selecting a modulator of ß-amyloid polymerization can similarly be used to select modulators that promote ß-AP aggregation. Thus, in the presence of a modulator that promotes ß-AP aggregation, the A405nm of the solution is increased compared to when the modulator is absent (e.g., the solution may exhibit an decreased lag time, increase slope of aggregation and/or a higher plateau level compared to when the modulator is absent). Another assay suitable for use in the screening method of the invention, a seeded extension assay, is also described further in Example 2. In this assay, ß-AP monomer and an aggregated ß-AP "seed" are combined, in the presence and absence of a test compound, and the amount of ß-fibril formation is assayed based on enhanced emission of the dye Thioflavine T when contacted with ß-AP fibrils. Moreover, ß-AP aggregation can be assessed by electron microscopy (EM) of the ß-AP preparation in the presence or absence of the modulator For example, ßamyloid fibril formation, which is detectable by EM, is reduced in the presence of a modulator that inhibits ß-AP aggregation (i.e, there is a reduced amount or number of ß-fibrils in the presence of the modulator), whereas ß fibril formation is increased in the presence of a modulator that promotes ß-AP aggregation (i.e., there is an increased amount or number of ß-fibrils in the presence of the modulator). Another preferred assay for use in the screening method of the invention to select suitable modulators is the neurotoxicity assay described in Example 3. Compounds are selected which inhibit the formation of neurotoxic Aß aggregates and/or which inhibit the neurotoxicity of preformed Aß fibrils This neurotoxicity assay is considered to be predictive of neurotoxicity in vivo. Accordingly, inhibitory activity of a modulator compound in the in vitro neurotoxicity assay is predictive of similar inhibitory activity of the compound for neurotoxicity in vivo V. Pharmaceutical Compositions Another aspect of the invention pertains to pharmaceutical compositions of the ß-amyloid modulator compounds of the invention. In one embodiment, the composition includes a ß amyloid modulator compound in a therapeutically or prophylactically effective amount sufficient to alter, and preferably inhibit, aggregation of natural ß-amyloid peptides, and a pharmaceutically acceptable carrier. In another embodiment, the composition includes a ß amyloid modulator compound in a therapeutically or prophylactically effective amount sufficient to inhibit the neurotoxicity of natural ß-amyloid peptides, and a pharmaceutically acceptable carrier. A "therapeutically effective amount" refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic result, such as reduction or reversal or ß-amyloid deposition and/or reduction or reversal of Aß neurotoxicity. A therapeutically effective amount of modulator may vary according to factors such as the disease state" age, sex, and weight of the individual, and the ability of the modulator to elicit a desired response in the individual. Dosage regimens may be adjusted to provide the optimum therapeutic response. A therapeutically effective amount is also one in which any toxic or detrimental effects of the modulator are outweighed by the therapeutically beneficial effects. The potential neurotoxicity of the modulators of the invention can be assayed using the cell-based assay described in Example 6 and a therapeutically effective modulator can be selected which does not exhibit significant neurotoxicity. In a preferred embodiment, a therapeutically effective amount of a modulator is sufficient to alter, and preferably inhibit, aggregation of a molar excess amount of natural ß-amyloid peptides. A "prophylactically effective amount" refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result, such as preventing or inhibiting the rate of ß-amyloid deposition and/or Aß neurotoxicity in a subject predisposed to ß-amyloid deposition. A prophylactically effective amount can be determined as described above for the therapeutically effective amount. Typically, since a prophylactic dose is used in subjects prior to or at an earlier stage of disease, the prophylactically effective amount will be less than the therapeutically effective amount. One factor that may be considered when determining a therapeutically or prophylactically effective amount of a ß amyloid modulator is the concentration of natural ß-AP in a biological compartment of a subject, such as in the cerebrospinal fluid (CSF) of the subject. The concentration of natural P-AP in the CSF has been estimated at 3 nM (Schwartzman, (1994)Proc Natl Acad Sci. USA 91:8368-8372). Anon-limiting range for a therapeutically or prophylactically effective amounts of a ß amyloid modulator is 0.01 nM-10 µM. It is to be noted that dosage values may vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that dosage ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed composition. The amount of active compound in the composition may vary according to factors such as the disease state, age, sex, and weight of the individual, each of which may affect the amount of natural P-AP in the individual. Dosage regimens may be adjusted to provide the optimum therapeutic response. For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the mammalian subjects to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on (a) the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals. As used herein "pharrnaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible. In one embodiment, the carrier is suitable for parenteral administration. Preferably, the earner is suitable for administration into the central nervous system (e g., intraspinally or intracerebrally). Alternatively, the carrier can be suitable for intravenous, intraperitoneal or intramuscular administration. In another embodiment, the carrier is suitable for oral administration. Pharrnaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. The use of such media and agents for pharrnaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the pharmaceutical compositions of the invention is contemplated. Supplementary active compounds can also be incorported into the compositions Therapeutic compositions typically must be sterile and stable under the conditions of manufacture and storage. The composition can be formulated as a solution, microemulsion, liposome, or other ordered structure suitable to high drug concentration. The earner can be a solvent or dispersion medium contaming, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetheylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as manitol, sorbitol, or sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, monostearate salts and gelatin. Moreover, the modulators can be administered in a time release formulation, for example in a composition which includes a slow release polymer The active compounds can be prepared with carriers that will protect the compound against rapid release, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, polylactic acid and polylactic, polyglycolic copolymers (PLG). Many methods for the preparation of such formulations are patented or generally known to those skilled in the art Sterile injectable solutions can be prepared by incorporating the active compound (e g, ß-amyloid modulator) in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. A modulator compound of the invention can be formulated with one or more additional compounds that enhance the solubnility of the modulator compound Prefered compounds to be added to formulations to enhance the solubility of the modulators are cyclodextrin derivatives, preferably hydroxypropyl--cyclodextrin. Drug delivery vehicles containing a cyclodextrin derivative for delivery of peptides to the central nervous system are described in Bodor, N , et al (1992) Science 257:1698-1700 For the ß-amyloid modulators described herein, inclusion in the formulation of hydroxypropyl-y-cyclodextrin at a concentration 50-200 mM increases the aqueous solubility of the compounds. In addition to increased solubility, inclusion of a cyclodextrin derivative in the formulation may have other beneficial effects, since |3-cyclodextrin itself has been reported to interact with the Ap peptide and inhibit fibril formation in vitro (Camilleri, P., et al (1994) FEBS Letters 34J_:256-258. Accordingly, use of a modulator compound of the invention in combination with a cyclodextrin derivative may result in greater inhibition of Aß aggregation than use of the modulator alone. Chemical modifications of cyclodextrins are known in the art (Hanessian, S., et al. (1995) J. Org. Chem. 60:4786-4797). In addition to use as an additive in a pharmaceutical composition containing a modulator of the invention, cyclodextrin derivatives may also be useful as modifying groups and, accordingly, may also be covalently coupled to an Aß peptide compound to form a modulator compound of the invention. Another preferred formulation for the modulator compounds to enhance brain uptake comprises the detergent Tween-80, polyethylene glycol (PEG) and ethanol in a saline solution. A non-limiting example of such a preferred formulation is 0.16% Tween-80,1.3% PEG-3000 and 2% ethanol in saline. In another embodiment, a pharmaceutical composition comprising a modulator of the invention is formulated such that the modulator is transported across the blood-brain barrier (BBB). Various strategies known in the art for increasing transport across the BBB can be adapted to the modulators of the invention to thereby enhance transport of the modulators across the BBB (for reviews of such strategies, see e g, Pardridge, W.M. (1994) Trends in Biotechnol. 12:239-245; Van Bree, J.B. et al. (1993) Pharm World Sci. 15:2-9; and Pardridge, W.M. et al (1992) Pharmacol. Toxicol 71_:3-10). In one approch the modulator is chemically modified to form prodrug with enhanced transmembrane transport. Suitable chemical modifications include covalent linking of a fatty acid to the modulator through an amide or ester linkage (see e.g., U.S. Patent 4,933,324 and PCT Publication WO 89/07938, both by Shashoua; U.S. Patent 5,284,876 by Hesse et al; Toth, I. et al. (1994) J Drug Target. 2.237-239; and Shashoua, V.E. et al (1984) J Med. Chem. 27.659-664) and glycating the modulator (see e g., U.S. Patent 5,260,308 by Poduslo et al.). Also, N-acylamino acid derivatives may be used in a modulator to form a "lipidic" prodrug (see e g, 5,112,863 by Hashimoto et al). In another approach for enhancing transport across the BBB, a peptidic or peptidomimetic modulator is conjugated to a second peptide or protein, thereby forming a chimeric protein, wherein the second peptide or protein undergoes absorptive-mediated or receptoT-mediated transcytosis through the BBB, Accordingly, by coupling the modulator to this second peptide or protein, the chimeric protein is transported across the BBB. The second peptide or protein can be a ligand for a brain capillary endothelial cell receptor ligand. For example, a preferred ligand is a monoclonal antibody that specifically binds to the transferrin receptor on brain capillary endothelial cells (see e.g, U.S. Patents 5,182,107 and 5,154,924 and PCT Publications WO 93/10819 and WO 95/02421, all by Friden et al). Other suitable peptides or proteins that can mediate transport across the BBB include histones (see e.g., U.S. Patent 4,902,505 by Pardridge and Schimmel) and ligands such as biotin, folate, niacin, pantothenic acid, riboflavin, thiamin, pryridoxal and ascorbic acid (see e g., U.S. Patents 5,416,016 and 5,108,921, both by Heinstein). Additionally, the glucose transporter GLUT-1 has been reported to transport glycopeptides (L-serinyl-ß-D-glucoside analogues of [Met5]enkephalin) across the BBB (Polt, R. etal. (1994)Proc. Natl. Acad. Sci. USA 9L7114-1778). Accordmgly, a modulator compound can be coupled to such a glycopeptide to target the modulator to the GLUT-1 glucose transporter. For example, a modulator compound which is modified at its amino terminus with the modifying group Aic (3-(0-aminoethyl-iso) cholyl, a derivative of cholic acid having a free amino group) can be coupled to a glycopeptide through the amino group of Aic by standard methods. Chimeric proteins can be formed by recombinant DNA methods (e g., by formation of a chimeric gene encoding a fusion protein) or by chemical crosslinking of the modulator to the second piptide or protein to form a chimeric protein Numerous chemical crosslinking agents are known in the (e g., commercially available from Pierce, Rockford IL). A crosslinking agent can be chosen which allows for high yield coupling of the modulator to the second peptide or protein and for subsequent cleavage of the linker to release bioactive modulator. For example, a bioun-avidin-based linker system may be used. In yet another approach for enhancing transport across the BBB, the modulator is encapsulated in a carrier vector which mediates transport across the BBB. For example, the modulator can be encapsulated m a liposome, such as a positively charged unilamellar liposome (see e.g , PCT Publications WO 88/07851 and WO 88/07852, both by Faden) or in polymeric microspheres (see e g., U.S. Patent 5,413,797 by Khan et al., U.S. Patent 5,271,961 by Mathiowitz et al and 5,019,400 by Gombotz et al). Moreover, the carrier vector can be modified to target it for transport across the BBB. For example, the carrier vector (e g., liposome) can be covalently modified with a molecule which is actively transported across the BBB or with a ligand for brain endothelial cell receptors, such as a monoclonal antibody that specifically binds to transferrin receptors (see e.g., PCT Publications WO 91/04014 by Collins et al. and WO 94/02178 by Greiget al). In still another approach to enhancing transport of the modulator across the BBB, the modulator is coadministered with another agent which functions to permeabilize the BBB. Examples of such BBB "permeabilizers" include bradykinin and bradykinin agonists (see e.g., U.S. Patent 5,112,596 by Malfroy-Camine) and peptidic compounds disclosed in U.S. Patent 5,268,164 by Kozarich et al Assays that measure the in vitro stability of the modulator compounds in cerebrospinal fluid (CSF) and the degree of brain uptake of the modulator compounds in animal models can be used as predictors of in vivo efficacy of the compounds. Suitable assays for measuring CSF stability and brain uptake are described in Examples 7 and 8, respectively. A modulator compound of the invention can be formulated into a pharmaceutical composition wherein the modulator is the only active compound or, alternatively, the pharmaceutical composition can contain additional active compounds. For example, two or more modulator compounds may be used in combination Moreover, a modulator compound of the invention can be combined with one or more other agents that have anti-amyloidogenic properties. For example, a modulator compound can be combined with the non-specific cholinesterase inhibitor tacrine (COGNEX®, Parke-Davis). In another embodiment, a pharmaceutical composition of the invention is provided as a packaged formulation. The packaged formulation may include a pharmaceutical composition of the invention in a container and printed instructions for administration of the composition for treating a subject having a disorder associated with ß-amyloidosis, e.g. Alzheimer's disease. VI. Methods of Using Aß Modulators Another aspect of the invention pertains to methods for altering the aggregation or inhibiting the neurotoxicity of natural ß-amyloid peptides. In the methods of the invention, natural ß amyloid peptides are contacted with a ß amyloid modulator such that the aggregation of the natural ß amyloid peptides is altered or the neurotoxicity of the natural ß amyloid peptides is inhibited. In a preferred embodiment, the modulator inhibits aggregation of the natural ß amyloid peptides. In another embodiment, the modulator promotes aggregation of the natural ß amyloid peptides. Preferably, aggregation of a molar excess amount of ß-AP, relative to the amount of modulator, is altered upon contact with the modulator. In the method of the invention, natural ß amyloid peptides can be contacted with a modulator either in vitro or in vivo. Thus, the term "contacted with" is intended to encompass both incubation of a modulator with a natural ß-AP preparation in vitro and delivery of the modulator to a site in vivo where natural P-AP is present. Since the modulator compound interacts with natural ß-AP, the modulator compounds can be used to detect natural ß-AP, either in vitro or in vivo Accordingly, one use of the modulator compounds of the invention is as diagnostic agents to detect the presence of natural ß-AP, either in a biological sample or in vivo in a subject. Furthermore, detection of natural AP- utilizing a modulator compound of the invention further can be used to diagnose amyloidosis in a subject. Additionally, since the modulator compounds of the invention disrupt ß-AP aggregation and inhibit ß-AP neurotoxicity, the modulator compounds also are useful in the treatment of disorders associated with ß-amyloidosis, either prophy tactically or therapeutically. Accordingly, another use of the modulator compounds of the invention is as therapeutic agents to alter aggregation and/or neurotoxicity of natural ß-AP. In one embodiment, a modulator compound of the invention is used in vitro, for example to detect and quantitate natural ß-AP in sample (eg.,a sample of biological fluid). To aid in detection, the modulator compound can be modified with a detectable substance. The source of natural ß-AP used in the method can be, for example, a sample of cerebrospinal fluid (e.g, from an AD patient, an adult susceptible to AD due to family history, or a normal adult). The natural ß-AP sample is contacted with a modulator of the invention and aggregation of the ß-AP is measured, such as by the assays described in Example 2. The degree of aggregation of the ß-AP sample can then be compared to that of a control sample(s) of a known concentration of ß-AP, similarly contacted with the modulator and the results can be used as an indication of whether a subject is susceptible to or has a disorder associated with ß-amyloidosis. Moreover, ß-AP can be detected by detecting a modulating group incorporated into the modulator. For example, modulators incorporating a biotin compound as described herein (e g, an amino-terminally biotinylated ß-AP peptide) can be detected using a streptavidin or avidin probe which is labeled with a detectable substance (e.g., an enzyme, such as peroxidase). In another embodiment, a modulator compound of the invention is used in vivo to detect, and, if desired, quantitate, natural ß-AP deposition in a subject, for example to aid in the diagnosis of ß amyloidosis in the subject. To aid in detection, the modulator compound can be modified with a detectable substance, preferably 99mTc or radioactive iodine (described further above), which can be detected in vivo in a subject. The labeled ß-amyloid modulator compound is administered to the subject and, after sufficient time to allow accumulation of the modulator at sites of amyloid deposition, the labeled modulator compound is detected by standard imaging techniques. The radioactive signal generated by the labeled compound car be directly detected (e.g, whole body-counting), or alternatively, the radioactive signal can be converted into an image on an autoradiograph or on a computer screen to allow for imaging of amyloid deposits in the subject. Methods for imaging amyloidosis using radiolabeled proteins are known in the art. For example, serum amyloid P component (SAP), radiolabeled with either 123I or 99mTc, has been used to image systemic amyloidosis (see e g, Hawkins, P.N. and Pepys, M.B. (1995) Eur. J. Nucl. Med. 22:595-599). Of the various isotypes of radioactive iodine, preferably 123I (half-life =13.2 hours) is used for whole body scintigraphy, 124I (half life = 4 days) is used for positron emission tomography (PET), 125I (half life = 60 days) is used for metabolic turnover studies and I31I (half life = 8 days) is used for whole body counting and delayed low resolution imaging studies. Analogous to studies using radiolabeled SAP, a labeled modulator compound of the invention can be delivered to a subject by an appropriate route (e.g, intravenously, intraspinally, intracerebrally) in a single bolus, for example containing 100 ug of labeled compound carrying approximately 180 MBq of radioactivity. The invention provides a method for detecting the presence or absence of natural ß-amyloid peptides in a biological sample, comprising contacting a biological sample with a compound of the invention and detecting the compound bound to natural ¸ß amyloid peptides to thereby detect the presence or absence of natural ß-amyloid peptides in the biological sample. In one embodiment, the ß-amyloid modulator compound and the biological sample are contacted in vitro In another embodiment, the ß-amyloid modulator compound is contacted with the biological sample by administering the ß-amyloid modulator compound to a subject For in vivo administration, preferably the compound is labeled with radioactive technetium or radioactive iodine. The invention also provides a method for detecting natural ß-amyloid peptides to facilitate diagnosis of a ß-amyloidogenic disease, comprising contacting a biological sample with the compound of the invention and detecting the compound bound to natural ß-amyloid peptides to facilitate diagnosis of a ß-amyloidogenic disease. In one embodiment, the ß-amyloid modulator compound and the biological sample are contacted in virto. In another ambodiment, the ß -amyloid modulator compound is contacted with the biological sample by administering the ß-amyloid modulator compound to a subject. For in vivo administration, preferably the compound is labeled with radioactive technetium or radioactive iodine. Preferably, use of the method facilitates diagnosis of Alzheimer's disease. In another embodiment, the invention provides a method for altering natural ß-AP aggregation or inhibiting ß-AP neurotoxicity, which can be used prophylactically or therapeutically in the treatment or prevention of disorders associated wim ß amyloidosis, e.g., Alzheimer's Disease. Modulator compounds of the invention can reduce the toxicity of natural ß-AP aggregates to cultured neuronal cells. Moreover, the modulators also have the ability to reduce the neurotoxicity of preformed AP fibrils. Accordingly, the modulator compounds of the invention can be used to inhibit or prevent the formation of neurotoxic AP fibrils m subjects {e.g., prophylactically in a subject predisposed to ß-amyloid deposition) and can be used to reverse ß-amyloidosis therapeutically in subjects already exhibiting ß-amyloid deposition. A modulator of the invention is contacted with natural ß amyloid peptides present in a subject (e.g, in the cerebrospinal fluid or cerebrum of the subject) to thereby alter the aggregation of the natural ß-AP and/or inhibit the neurotoxicity of the natural ß-APs. A modulator compound alone can be administered to the subject, or alternatively, the modulator compound can be administered in combination with other therapeutically active agents (e g., as discussed above in subsection IV). When combination therapy is employed, the therapeutic agents can be coadministered in a single pharmaceutical composition, coadministered in separate pharmaceutical compositions or administered sequentially The modulator may be administered to a subject by any suitable route effective for inhibiting natural P-AP aggregation in the subject, although in a particularly preferred embodiment, the modulator is administered parenterally, most preferably to the central nervous system of the subject. Possible routes of CNS administration include intraspinal administration and intracerebral administration (e.g., intracerebrovascular administration). Alternatively, the compound can be administered, for example, orally, Inlraperitoneally, intravenously or intramuscularly.For non CNS administration routes, the compound can be administered in a formulation which allows for transport across the BBB. Certain modulators may be transported across the BBB without any additional further modification whereas others may need further modification as described above in subsection IV. Suitable modes and devices for delivery of therapeutic compounds to the CNS of a subject are known in the art, including cerebrovascular reservoirs (e.g., Ommaya or Rikker reservoirs; see e.g., Raney, J.P. etal (1988) J. Neurosci. Nurs. 20:23-29; Sundaresan, N. etal. (1989) Oncology 3:15-22), catheters for intrathecal delivery (e g, Port-a-Cath, Y-catheters and the like; see e.g., Plummer, J.L. (1991) Pain 44:215-220; Yaksh, T.L. et al. (1986) Pharmacol. Bwchem. Behav. 25:483-485), injectable intrathecal reservoirs (e.g, Spinalgesic; see e g, Brazenor, G.A. (1987) Neurosurgery 2^:484-491), implantable infusion pump systems (e g , Infusaid; see e.g., Zierski, J. et al. (1988) Acta Neurochem. Suppl. 43/94-99; Kanoff, R.B. (1994) J. Am. Osteopath. Assoc. 94-487-493) and osmotic pumps (sold by Alza Corporation). A particularly preferred mode of administration is via an implantable, externally programmable infusion pump. Suitable infusion pump systems and reservoir systems are also described in U.S. Patent No. 5, 368,562 by Blomquist and U.S. Patent No. 4,731,058 by Doan, developed by Pharmacia Deltec Inc The method of the invention for altering ß-AP aggregation in vivo , and in particular for inhibiting ß-AP aggregation, can be used therapeutically in diseases associated with abnormal ß amyloid aggregation and deposition to thereby slow the rate of ß amyloid deposition and/or lessen the degree of ß amyloid deposition, thereby ameliorating the course of the disease. In a preferred embodiment, the method is used to treat Alzheimer's disease (e.g., sporadic or familial AD, including both individuals exhibiting symptoms of AD and individuals susceptible to familial AD). The method can also be used prophylactically or therapeutically to treat other clinical occurrences of ß amyloid deposition, such as in Down's syndrome individuals and in patients with hereditary cerebral hemorrhage with amyloidosis-Dutch-type (HCHWA-D). While inhibition of p-AP aggregation is a preferred therapeutic method, modulators that promote p-AP aggregation may also be useful therapeutically by allowing forme sequestration of p-AP at sites that do not lead to neurological impairment. Additionally, abnormal accumulation of p-amyloid precursor protein in muscle fibers has been implicated in the pathology of sporadic inclusion body myositis (IBM) (Askana, V. et al. (1996) Proc. Natl Acad Sa USA 93:1314-1319; Askanas, V et al. (1995) Current Opinion in Rheumatology 7:486-496). Accordingly, the modulators of the invention can be used prophylactically or therapeutically in the treatment of disorders in which p-AP, or APP, is abnormally deposited at non-neurological locations, such as treatment of IBM by delivery of the modulators to muscle fibers. This invention is further illustrated by the following examples which should not be construed as limiting. A modulator's ability to alter the aggregation of natural p-amyloid peptide and/or inhibit the neurotoxicity of natural p-amyloid peptide in the assays described below are predictive of the modulator's ability to perform the same function in vivo. This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patents and published patent applications cited throughout this application, as well as the Figures, are hereby incorporated by reference. EXAMPLE 1: Preparation of ß-amyloid Modulator Compounds Comprising D-Amino Acids p-amyloid modulators comprising D-amino acids can be prepared by solid-phase peptide synthesis, for example using an Nα-9-fluorenylmethyloxycarbonyl (FMOC)-based protection strategy as follows. Starting with 2.5 mmoies of FMOC-D-Val-Wang resin, sequential additions of each amino acid are performed using a four-fold excess of protected amino acids, l-hydroxybenzotnazole (HOBt) and diisopropyl carbodiimide (DIC). Recouplings are performed when necessary as determined by ninhydrin testing of the resin after coupling. Each synthesis cycle is minimally described by a three minute deprotection(25 % piperidine /Nmethyl-pyrrolidone (NMP)), a 15 minute deprotection, five one minute NMP washes, a 60 minute coupling cycle, five NMP washes and a ninhydrin test. For N-terminal modification, an N-terminal modifying reagent is substituted for an FMOC-D-amino acid and coupled to a 700 mg portion of the fully assembled peptide-resin by the above protocol. The peptide is removed from the resin by treatment with trifluoroacetic acid (TFA) (82.5 %), water (5 %), thioanisole (5 %), phenol (5 %), ethanedithiol (2.5 %) for two hours followed by precipitation of the peptide in cold ether. The solid is pelleted by centrifugation (2400 rpm x 10 mm.), and the ether decanted. The solid is resuspended in ether, pelleted and decanted a second time. The solid is dissolved in 10 % acetic acid and lyophilized to dryness. For preparative purification and subsequent analytical characterization, 60 mg of the solid is dissolved in 25 % acetonitrile (ACN) /0.1 % TFA and applied to a C18 reversed phase high performance liquid chromatography (HPLC) column. Alternatively, ß-amyloid modulators comprising D-amino acids can be prepared on a Rainin PS3 peptide synthesizer using an automated protocol established by the manufacturer for a 0.25 mmole scale synthesis Couplings are performed using 2-(lH-benzotriazol-l-yl)-l5l,3,3-tetramethyl-uroruum-hexafluoro-phosphate (HBTU) /FMOC-D-amino acid in four fold excess in 0 4 M N-methylmorpholine (NMM) / dimethylformamide (DMF) for 60 minutes. In between couplings, the FMOC group is removed by reaction with 20% pipendme / DMF for 20 minutes. The peptide is removed from the resin by treatment with 95% TF A/water for one hour and precipitated with ether. The pellet is resuspended in 40% acetonitrile/water and Iyophilized. When necessary, the material was purified by preparative HPLC using 15%-50% acetomtnle over 60 minutes on a Vydac CI8 column (21 x 250 mm). Various N-terminally modified p-amyloid modulator compounds can be synthesized using standard methods. Fully-protected resin-bound peptides are prepared as described above on an appropriate resin to eventually afford carboxyl terminal peptide acids. Small portions of each peptide resin (e g , 13-20 µmoles) are aliquoted into separate reaction vessels. The TV-terminal FMOC protecting group of each sample is removed in the standard manner with 20 % piperidine in NMM followed by extensive washig with DMF The unprotected N-terminal α-amino group of e ach peptide-resin sample can be modified using one of the following methods: Method A, coupling of modifying reagents containing free carboxylic acid groups: The modifying reagent (five equivalents) is predissolved in NMP, DMSO or a mixture of these two solvents. HOBT and DIC (five equivalents of each reagent) are added to the dissolved modifier and the resulting solution is added to one equivalent of free-amino peptide-resin. Coupling is allowed to proceed overnight, followed by washing. If a ninhydrin test on a small sample of peptide-resin shows that coupling is not complete, the coupling is repeated using 1 -hydroxy-7-azabenzotriazole (HOAt) in place of HOBt. Method B, coupling of modifying reagents obtained in preactivated forms. The modifying reagent (five equivalents) is predissolved in NMP, DMSO or a mixture of these two solvents and added to one equivalent of peptide-resin. Diisopropylethylamine (DIEA; six equivalents) is added to the suspension of activated modifier and peptide-resin. Coupling is allowed to proceed overnight, followed by washing. If a ninhydrin test on a small sample of peptide-resin shows that coupling is not complete, the coupling is repeated. After the second coupling (if required) the N-terminaily modified peptide-resins are dried at reduced pressure and cleaved from the resin with removal of side-chain protecting groups as described above. Analytical reversed-phase HPLC is used to confirm that a major product is present in the resulting crude peptides, which are purified using Millipore Sep-Pak cartridges or preparative reverse-phase HPLC. Mass spectrometry or high-field nuclear magnetic resonance spectrometry is used to confirm the presence of the desired compound in the product. Method C, preparation of N-terminal-alkyl substituted peptides using bromoacetyl peptide intermediates: A resin-bound peptide can be coupled to bromoacetic acid (12 equivalents) with 1,3-dnsopropyIcarbodiimide (D1C) (13 equivalents) in DMF. The resulting bromoacetyl substituted peptide can be modified upon reaction with primary or secondary amines including, methylamine, ethylamine, propylamine, isopropylamine and piperidine. The reaction is performed in 60% DMSO/DMF and is typically complete after 24 hours Method D, preparation of N-terminal-alkyl substituted peptides via reductive alkylation: After the peptide is dissolved (or partially dissolved) in water containing 0-10 % methanol, it is reacted with an aldehyde (5-8 equivalents) and sodiumcyanoborohydride (10-16 equivalents). The number of equivalents can be adjusted for the type of aldehyde and the degree of substitution desired. The pH of the resulting solution is adjusted to 2 with 1 M HC1 and maintained at 2 for one hour. The reaction is monitored by hplc and is usually completed with two hours The reaction mix is concentrated at room temperature and hplc purified. Method E, C-terminal modification: The peptide was synthesized on 2-chlorotrityl resin using standard Fmoc chemistry however the final D-amino acid group coupled was Boc protected. The peptide was removed from the resin with 8/1/1 dichloromethane (DCM) / acetic acid / tnfluoroethanol and the mixture concentrated. The peptide residue was dissolved in 20 % acetonitrile, frozen and lyophilyzed overnight. The crude BOC protected peptide acid was coupled under basic conditions (pH=l 1, adjusted with DIE A) to an amine with one equivalent each of l-hydroxy-7- azobenzotriazole(HOAt) and DIC. The reaction was completed after stirring overnight and the peptide precipitated with water. The BOC group was cleaved upon reaction with 25 % TFA in DCM for one hour and the peptide HPLC purified. EXAMPLE 2: ß-Amyloid Aggregation Assays The ability of ß-amyloid: modulator compounds to modulate (e g, inhibit or promote) the aggregation of natural P-Aß when combined with the natural ß-AP can be examined in one or both of the aggregation assays described below. Natural ß-AP (ß-Aß1-40) for use in the aggregation assays is commercially available from Bachem (Torrance, CA). A. Nucleation Assay The nucleation assay is employed to determine the ability of test compounds to alter (e.g inhibit) the early events in formation of ß-AP fibers from monomeric ß-Aß. Characteristic of a nucleated polymerization mechanism , a lag time is observed prior to nucleation, after which the peptide rapidly forms fibers as reflected in a linear rise in turbidity. The time delay before polymerization of P-Aß monomer can be quantified as well as the extent of formation of insoluble fiber by light scattering (turbidity). Polymerization reaches equilibrium when the maximum turbidity reaches a plateau. The turbidity of a solution of natural ß-AP m the absence or presence of various concentrations of a ß-amyloid modulator compound is determined by measuring the apparent absorbance of the solution at 405nm (A405 nm) over time. The threshold of sensitivity for the measurement of turbidity is in the range of 15-20 µM P-Aß. A decrease in turbidity over time in the presence of the modulator, as compared to the turbidity in the absence of the modulator, indicates that the modulator inhibits formation of ß-AP fibers from monomeric ß-AP. This assay can be performed using stirring or shaking to accelerate polymerization, thereby increasing the speed of the assay. Moreover the assay can be adapted to a 96-well plate format to screen multiple compounds. To perform the nucleation assay, first Aß1-40 peptide is dissolved in HFIP (l,l,l,3,3,3-Hexafluoro-2-propanol; Aldrich 10,522-8) at a concentration of 2 mg peptide/ml and incubated at room temperature for 30 min. HFIP-solubilized peptide is sonicated in a waterbath sonicator for 5 min at highest setting, then evaporated to dryness under a stream of argon. The peptide film is resuspended in anhydrous dimethylsulfoxide (DMSO) at a concentration of 6.9 mg/ml (25x concentration), sonicated for 5 min as before, then filtered through a 0.2 micron nylon syringe filter (VWR cat. No. 28196-050). Test compounds are dissolved in DMSO at a lOOx concentration. Four volumes of 25x Aß1-40 peptide in DMSO are combined with one volume of test compound in DMSO in a glass vial, and mixed to produce a 1:1 molar ratio of Aß peptide to test compound. For different molar ratios, test compounds are diluted with DMSO prior to addition to Aß1.40, in order to keep the final DMSO and Aß1-40 concentrations constant Control samples do not contain the test compound. Ten microliters of the mixture is then added to the bottom of a well of a Corning Costar ultra low binding 96-well pJate (Corning Costar, Cambridge MA; cat. No. 2500) Ninety microliters of water is added to the well, the plate is shaken on a rotary shaken at a constant speed at room temperature for 30 seconds, an additional 100 u.1 of 2x PTL buffer (20 mM 'NaH2P04, 300 mM NaCl, pH 7.4) is added to the well, the plate is reshaken for 30 seconds and a baseline (t=0) turbidity reading is taken by measuring the apparent absorbance at 405 nm using a Bio-Rad Model 450 Microplate Reader. The plate is then returned to the shaker and shaken continuously for 5 hours. Turbidity readings are taken at 15 minute intervals ß-amyloid aggregation in the absence of any modulators results in enhanced turbidity of the natural (3-Aß solution (1 e , an increase in the apparent absorbance at 405 run over time). Accordingly, a solution including an effective inhibitory modulator compound exhibits reduced turbidity as compared to the control sample without the modulator compound {i.e., less apparent absorbance at 405 nm over time as compared to the control sample). Alternative to use of turbidity to quantitate ß-amyloid aggregation, fluorescence of thioflavin T (Th-T) also can be used to quantitate ß-amyloid aggregation in the nucleation assay (use of Th-T fluorescence for quantitating ß-amyloid aggregation is described further below for the seeded extension assay) B. Fibril Binding Assay The following materials are needed for the Fibril binding assay: Millipore multifilter apparatus; 12 x 75 glass tubes; GF/F 25 mm glass filters; PBS/0.1% tween 20 at 4°C (PBST); Aß fibrils; radioactive compound; nonradioactive compound; Eppendorf repeat pipettor with tips; labels; forceps; and vacuum. In this assay, each sample is run in triplicate. The "aged" Aß fibril is first prepared approximately 8 days in advance by aging 1 ml aliquots of a 200 µM Aß 1-40 peptide solution in 4%DMSO/PBS for 8 days at 37°C with rocking. Such "aged" Aß peptide can be tested directly on cells or frozen at -80°C. The 200 µM Aß fibril is diluted in PBST to yield a 4 µM solution (320 µ1 in 16 ml PBST). 100µL aliquots of this solution are added per tube with the repeat pipetor The p-amyloid modulator compounds of the invention are prepared at 2|iM - 200 fM dilutions as follows- Dilute a 5 mM stock 1:3 in DMSO to yield a 1.6667 stock (200 µl in 400 µl DMSO). Dilute a 1.667 mM stock 1:3 in DMSO to yield a 0.5556 stock (200 µl in 400 µl DMSO). Dilute a 555.556 µM stock 1:3 in DMSO to yield a 185.19 stock (200 µl in 400 µl DMSO). 5 Dilute a 185.185 µM stock 1:3 in DMSO to yield a 61.728 stock (200 µl in 400 µl DMSO). Dilute a 61.728 µM stock 1:3 in DMSO to yield a 20.576 stock (200 µl in 400 µl DMSO). Dilute a 20.576 µM stock 1:3 in DMSO to yield a 6.8587 stock (200 µl in 400 µl DMSO). Dilute a 6.859 µM stock 1:3 in DMSO to yield a 2.2862 stock (200 µl in 400 µl DMSO) Dilute a 2.286 µM stock 1:3 in DMSO to yield a 0.7621 stock (200 µl in 400 µl DMSO) 10 Dilute a 762.079 nM stock 1:3 in DMSO to yield a 254.03 stock (200 µl in 400 µl DMSO). Dilute a 254.026 nM stock 1:3 in DMSO to yield a 84.675 stock (200 µl in 400 µl DMSO). Dilute a 84.675 nM stock 1:3 in DMSO to yield a 28.225 stock (200 µl in 400 µl DMSO). Dilute a 28.225 nM stock 1:3 in DMSO to yield a 9.4084 stock (200 µl in 400µl DMSO). Dilute a 9.408 nM stock 1:3 in DMSO to yield a 3.1361 stock (200 µl in 400 µl DMSO) 15 Dilute a 3.136 nM stock 1:3 in DMSO to yield a 1.0454 stock (200 µl in 400 µl DMSO). Dilute a 1.045 nM stock 1:3 in DMSO to yield a 0.3485 stock (200 µl in 400 µl DMSO). Dilute a 348.459 µM stock 1:3 in DMSO to yield a 116.15 stock (200 µl in 400 µl DMSO). Dilute a 116.153 µM stock 1:3 m DMSO to yield a 38.718 stock (200 µl in 400 µl DMSO). Dilute 185.185 µM stock 1:25 in PBST to yield 7.4074 (50 µL m 1.2 mL PBST) 20 Dilute 61.728 µM stock 1:25 in PBST to yield 2.4691 (50 µL in 1.2 mL PBST) Dilute 20.576 µM stock 1:25 in PBST to yield 0.823 (50 µL in 1.2 ml PBST) Dilute 6.859 µM stock 1:25 in PBST to yield 0.2743 (50 µL in 1.2 ml PBST) Dilute 2.286 µM stock 1:25 in PBST to yield 0.0914 (50 µL in 1.2 mL PBST) Dilute 762.079 nM stock 1:25 in PBST to yield 30.483 (50 µL in 1.2 mL PBST) 25 Dilute 254.026 nM stock 1:25 in PBST to yield 10.161 (50 µL in 1.2 mL PBST) Dilute 84.675 nM stock 1:25 in PBST to yield 3.387 (50 µL in 1.2 mL PBST) Dilute 28.225 nM stock 1:25 in PBST to yield 1.129 (50 µL in 1.2 mL PBST) Dilute 9.408 nM stock 1:25 in PBST to yield 0.3763 (50 µL in 1.2 mL PBST) Dilute 3 136 nM stock 1:25 in PBST to yield 0 1254 (50 µL m 1.2 mL PBST) 30 Dilute 1 045 nM stock 1:25 in PBST to yield 0 0418 (50 uL in 1.2 mL PBST) Dilute 348.459 pM stock 1:25 in PBST to yield 13.938 (50 uL in 1.2 mL PBST) Dilute 116.153 pM stock 1:25 in PBST to yield 4.6461 (50 uL in 1.2 mL PBST) The ß-amyloid modulator compound (200µ.L) is then added to the appropriate tube containing the Aß fibril. The radioactively labeled ß-amyloid modulator compound is prepared using standard radioactive safety protocols by making a dilution into a PBS/0.1% tween-20 solution such that there is a final concentration of 20,000 dpm per 100 µl. 100 µ1 aliqouots of the radioactively labeled ß-amyloid modulator compound are added per tube using the repeat pipettor. The samples are covered with parafllm and incubated at 37 °C inside plastic radioactivity bags overnight. To filter the samples, the filters are pre-wetted in a small volume of PBST Two Millipore multifiltration apparati are set with GF7F filters in each filtration slot following the instructions from the manufacturer. The samples are removed from the 37 °Cjncubator and each sample is filtered using a small volume (~5 ml) of cold PBST buffer. The sample tube is then washed with two additional 5 mL volumes of cold PBST buffer. The vacuum is allowed to pull to a semi dry filter for approximately 2 minutes after adding the last sample and the filter is transferred to a labelled tube for iodination counting. One minute counts are recorded, the data is plotted, and the Prism program (GraphPAD) is used to analyze the graph, according to the manufacturer's instrutions. C. Seeded Extension Assay The seeded extension assay can be employed to measure the rate of Aß fiber formed in a solution of Aß monomer following addition of polymeric Aß fiber "seed" The ability of test compounds to prevent further deposition of monomelic Aß to previously deposited amyloid is determined using a direct indicator of P-sheet formation using fluorescence. In contrast with the nucleation assay, the addition of seed provides immediate nucleation and continued growth of preformed fibrils without the need for continuous mixing, and thus results in the absence of a lag time before polymerization starts. Since this assay uses static polymerization conditions, the activity of positive compounds in die nucleation assay can be confirmed in this second assay under different conditions and with an additional probe of amyloid structure. In the seeded extension assay, monomeric Aß1-40 is incubated in the presence of a "seed" nucleus (approximately ten mole percent of Aß that has been previously allowed to polymerize under controlled static conditions). Samples of the solution are then diluted in thioflavin T (Th-T). The polymer-specific association of Th-T with Aß produces a fluorescent complex that allows the measurement of the extent of fibril formation (Levine, H. (1993) Protein Science 2:404-410). In particular, association of Th-T with aggregated p-Aß, but not monomeric or loosely associated p-Aß, gives nse to a new excitation (ex) maximum at 450 ran and an enhanced emission (em) at 482 nm, compared to the 385 nm (ex) and 445 nm (em) for the free dye. Small aliquots of the polymerization mixture contain sufficient fibril to be mixed with Th-T to allow the monitoring of the reaction mixture by repeated sampling. A linear growth curve is observed in the presence of excess monomer. The formation of thioflavin T responsive P-sheet fibrils parallelsthelncrease in t urbidity observed using the nucleation eassy A solution of Aß monomer for use in the seeded extension assay is prepared by dissolving an appropriate quantity of Aß1-40 peptide in 1/25 volume of dimethysulfoxide (DMSO), followed by water to 1/2 volume and 1/2 volume 2x PBS (lOx PBS: NaCl 137 mM, KC1 2.7 mM Na2HP04 • 7H20 4.3 mM, KH2PO4 1.4 mM pH 7.2) to a final concentration of 200 uM. To prepare the stock seed, 1 ml of the Aß monomer preparation, is incubated for approximately 8 days at 37 °C and sheared sequentially through an 18, 23, 26 and 30 gauge needle 25,25, 50, and 100 times respectively. 2 µl samples of the sheared material is taken for fluorescence measurements after every 50 passes through the 30 gauge needle until the fluorescence units (FU) plateau (approx. 100-150x). Test compounds are prepared by dissolving an appropriate amount of test compound in lx PBS to a final concentration of 1 mM (lOx stock). If insoluble, the compound is dissolved in 1/10 volume of DMSO and diluted in lx PBS to 1 mM. A further 1/10 dilution is also prepared to test each candidate at both 100 µM and 10 uM. To perform the seeded extension assay, each sample is set up with 50 p.1 of 200 uM monomer, 125 FU sheared seed (a variable quantity dependent on the batch of seed, routinely 3-6 ul) and 10 ul of 10x modulator solution. The sample volume is then adjusted to a final volume of 100 µl with lx PBS. Two concentrations of each modulator typically are tested: 100 µM and 10 uM, equivalent to a 1:1 and a 1:10 molar ratio of monomer to modulator. The controls include an unseeded reaction to confirm that the fresh monomer contains ho seed, and a seeded reaction in the absence of any modulators, as a reference to compare against candidate modulators. The assay is incubated at 37 °C for 6 h, taking 2 ul samples hourly for fluorescence measurements. To measure fluorescence, a 2 ul sample of Aß is added to 400 µ1 of Thioflavin-T solution (50 mM Potassium Phosphate 10 mM Thioflavin-T pH 7.5). The samples are vortexed and the fluorescence is read in a 0.5 ml micro quartz cuvette at EX 450 nm and EM 482 nm (Hitachi 4500 Fluorimeter) ß-amyloid aggregation results in enhanced emission of Thioflavin-T. Accordingly, samples including an effective inhibitory modulator compound exhibit reduced emission as compared to control samples without the modulator compound. EXAMPLE 3: Analysis of ß-Amyloid Modulator Compounds In this example, ß-amyloid modulator compounds described herein were prepared and tested for their ability to inhibit aggregation of natural ß-amyloid peptide using aggregations assays as described m Example 2. The results from a first series of experiments, are summarized below in Tables I, II, and III. TABLE I Notes: * means nucleation assay data was measured at 3, 1 and 0.3 uM of compound # means nucleation assay data was measured at 2.5, 1.25 and 0.6 pM of compound (Table Removed) TABLE II (Table Removed) Notes: * means nucleation assay data was measured at 2.5, 1.25 and 0.6 (J.M of compound The modulator compounds were evaluated in nucleation assays utilizing 5 uM A|3|_4o and either 5 jxM, 2.5 uM, 1.25 uM, 3 uM, 1 uM, or 0.3 uM test compound. The change in lag time (ALag) is presented as the ratio of the lag time observed in the presence of the test compound (at either 5 uM, 2.5 uM, 1.25 uM, 3 uM, 1 uM, or 0.3 jiM) to the lag time of the control. TABLE III (Table Removed) (nvl) = D-norvaline (nle) = D-norleucine [3-I]y = 3-iodo-D-tyrosine [p-F]f=para-fiuoro-D-phenylalanine PPI-1801 is the acetyl amide analog of H-LPFFD-OH that has been reported in the literature. This compound was prepared and tested for activity for comparison purposes. The results indicate that this compound binds poorly to fibrils in the assay used herein. In contrast, the results shown in Tables I, II, and III, and Figure 2 demonstrate that p-amyloid modulators of the invention are effective inhibitors of Aß aggregation. EXAMPLE 6: Neurotoxicity Assay The neurotoxicity of natural p-amyloid peptide aggregates, in either the presence or absence of a (3-amyloid modulator, calTBeTe^te^lifTcell-based assay usin^eraierar rat or human neuronally-derived cell line (PC-12 cells or NT-2 cells, respectively) and the viability indicator 3,(4,4-dimethylthiazol-2-yl)2,5-diphenyl-tetra2olium bromide (MTT) (See e.g., Shearman, M.S. et al (1994) Proc. Natl. Acad. Set USA 91_:1470-1474; Hansen, M.B. et al. (1989) J. Immun. Methods JJ9:203-210 for a description of similar cell-based viability assays). PC-12 is a rat adrenal pheochromocytoma cell line and is available from the American Type Culture Collection, Rockville, MD (ATCC CRL 1721). MTT (commercially available from Sigma Chemical Co.) is a chromogenic substrate that is converted from yellow to blue m viable cells, which can be detected spectrophotometrically. To test the neurotoxicity of natural (3-amyloid peptides, stock solutions of fresh Aß monomers and aged Aß aggregates are first prepared. AßI_4Q in 100% DMSO is prepared from lyophilized powder and immediately diluted in one half the final volume in H2O and then one half the final volume in 2X PBS so that a final concentration of 200 p.M peptide, 4% DMSO is achieved. Peptide prepared vn this way and tested immediately on cells is referred to as "fresh" Aß monomer. To prepare "aged" Aß aggregates, peptide solution is placed in a 1.5 ml Eppendorf tube and incubated at 37 °C for eight days to allow fibrils to form. Such "aged" Aß peptide can be tested directly on cells or frozen at -80°C. The neurotoxicity of fresh monomers and aged aggregates are tested using PC12 and NT2 cells. PC12 cells are routinely cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% horse serum, 5% fetal calf serum, 4mM glutamine, and 1% gentamycin. NT2 cells are routinely cultured in OPTI-MEM medium (GIBCO BRL CAT. #31985) supplemented with 10% fetal calf serum, 2 mM glutamine and 1% gentamycin. Cells are plated at 10-15,000 cells per well in 90 µl of fresh medium in a 96 -well tissue culture plate 3-4 hours prior to treatment. The fresh or aged Aß peptide solutions (10 µL) are then diluted 1:10 directly into tissue culture medium so that the final concentration is in the range of 1-10 µM peptide. Cells are incubated in the presence of peptide without a change in media for 48 hours at 37°C. For the final three hours of exposure of the cells to the P-AP preparation, MTT is added to the media to a final concentration of 1 mg/ml and incubation is continued at 37 °C. Following the two hour incubation with MTT, the media is removed and the cells are lysed in 100 µL isopropanol/0.4N HCl with agitation. An equal volume of PBS is added to each well and the plates are agitated for an additional 10 minutes. Absorbance of each well at 570 nm is measured using a microliter plate reader to quantitate viable cell. Using this assay, the neurotoxicity of aged (5 day or 8 day) Aß1-40 aggregates alone, but not fresh Aß1-40 monomers alone, was confirmed. Experiments demonstrated that incubating the neuronal cells with increasing amounts of fresh Aß1-40 monomers was not significantly toxic to the cells whereas incubating the cells with increasing amounts of 5 day or 8 day Aß1-40 aggregates led to increasing amount of neurotoxicity. The EC50 for toxicity of aged Aß1-40 aggregates was 1 -2 µM for both the PC12cells and the NT2 cells. To determine the effect of a ß-amyloid modulator compound on the neurotoxicity of Aß1-40 aggregates, a modulator compound is preincubated with Aß1-40 monomers under standard nucleation assay conditions as described in Example 2 and at particular time intervals post-incubation, aliquots of the ß-AP/modulator solution are removed and 1) the turbidity of the solution is assessed as a measure of aggregation and 2) the solution is applied to cultured neuronal cells for 48 hours at which time cell viability is assessed using MTT to determine the neurotoxicity of the solution. Additionally, the ability of p-amyloid modulator compounds to reduce the neurotoxicity of preformed Aß1-40 aggregates can be assayed In these experiments, Aß1-40 aggregates are preformed by incubation of the monomers in the absence of any modulators. The modulator compound is then incubated with the preformed Aß1-40 aggregates for 24 hours at 37 °C, after which time the p-AP/modulator solution is collected and its neurotoxicity evaluated as described above. EXAMPLE 7: Assay of Modulator Compound Stability in Cerebrospinal Fluid The stability of a modulator compound in cerebrospinal fluid (CSF) can be assayed in an in vitro assay as follows. A CSF solution is prepared containing 75% Rhesus monkey CSF (commercially available from Northern Biomedical Research), 23% sterile phosphate buffered saline and 2% dimethylsulfoxide (v/v) (Aldrich Chemical Co., Catalog No. 27,685-5). Test modulator compounds are added to the CSF solution to a final concentration of 40 µM or 15 µM. All sample handling is carried out in a laminar flow hood and test solutions are maintained at 37 °C during the assay. After 24 hours, enzymatic activity in the solutions is quenched by adding acetonitnle to produce a final concentration of 25% (v/v). Samples (at the 0 time point and the 24 hour time point) are analyzed at room temperature using reverse-phase HPLC. A microbore column is used to maximize sensitivity. The parameters for analytical HPLC are as follows: Solvent System A: 0.1% Trifluoroacetic acid (TFA) in water (v/v) B: 0.085% TFA/Acetonitrile. 1% H20 (v/v) Injection and Gradient Inject: 100-250 µL of test sample Run: 10% for B for 5 min., then 10-70% B over 60 min. Chromatographic analysis is performed using a Hewlett Packard 1090 series II HPLC. The column used for separation is a C4, 5 urn, 1 x 250 mm (Vydac #214TP51) The flow rate is 50 µL/min and the elution profile of the test compounds is monitored at 214, 230, 260 and 280 nm. EXAMPLE 8: Brain Uptake Assay Brain levels of our Aß-derived peptides were determined in the rat following intravenous administration. Under ketamine/xylazine anesthesia male Sprague-Dawley rats (219-302g) received an intravenous injection via a catheter inserted in the left jugular vein (dose volume of 4 mL/kg administered over 1 minute) The actual dose administered of each compound tested is shown in Figure 1. At 60 minutes post administration the left common carotid artery was cannulated to enable perfusion of the left forebrain to remove cerebral blood. The left forebrain, void of blood was subjected to capillary depletion as described by (Triguero et al. (1990) J Neurochem. 54:1882-1888). This established technique separates brain vasculature from the parenchyma and, thus, allows the accurate determination of the concentration of compound under investigation that has traversed the blood brain barrier. The amount of parent compound that was present within the brain was determined by LC/MS/MS. The above-described assay was used to measure the bram uptake of the following modulators: (Table Removed) *The lower letter notation refers to a D-configuration The results are summarized in Figure 1. The (3-amyloid modulator compounds described herein are summarized in the following Table. TABLE IV (Table Removed) EQUIVALENTS Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims. We claim: 1. A ß-amyloid modulator compound comprising the structure: N-mehtyl-(D-Leu-D-Val-D-Phe-D-Leu)-NH2. 2. A pharmaceutical composition for treating or diagnosing Alzheimer's disease comprising the ß-amyloid modulator compound as claimed in claim 1 at a concentration of 0.01 nM to 10 µM and a pharmaceutically acceptable carrier.

Documents:

in-pct-2001-00886-del-abstract.pdf

in-pct-2001-00886-del-claims.pdf

in-pct-2001-00886-del-correspondence-others.pdf

in-pct-2001-00886-del-correspondence-po.pdf

in-pct-2001-00886-del-description (complete).pdf

in-pct-2001-00886-del-drawings.pdf

in-pct-2001-00886-del-form-1.pdf

in-pct-2001-00886-del-form-19.pdf

in-pct-2001-00886-del-form-2.pdf

in-pct-2001-00886-del-form-3.pdf

in-pct-2001-00886-del-form-5.pdf

in-pct-2001-00886-del-gpa.pdf

in-pct-2001-00886-del-petition-137.pdf

in-pct-2001-00886-del-petition-138.pdf