Title of Invention

CREAM FILLER COMPOSITION AND METHOD FOR PREPARING.

Abstract

The invention relates to a cream filler composition, which comprises: - a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction, - a powder sweetener composition, - and a wheat gluten fraction having an increase gliadin content compared to the natural gliadin content in wheat gluten. The invention relates further to a method for preparing such a cream filler composition and food compositions comprising such a cream filler composition.

Full Text

CREAM FILLER COMPOSITION AND METHOD FOR PREPARING The invention relates to a cream filler composition, a method for preparing such a cream filler composition and food compositions comprising such a cream filler composition. Cream filler compositions for sandwich cookies are generally composed of sucrose, or sucrose in combination with other sweeteners, flavourings, and oils or fats. These oils, fats or oil/fat mixtures (hereafter called oleaginous compositions) are very important with regard to the organoleptic properties of the cream, but also towards texture and storage stability. In order to obtain these properties, these oleaginous compositions are formulated by combining different fats and oils, which have been wholly or partially hydrogenated. As a result thereof, the oleaginous component of the cream filling comprises an increased quantity of saturated fatty acids in combination with trans-fatty acids. Typical examples of such cream fillings are described in US 3,244,536, US 4,834,991 or US 4,753,812. In US 3,244,536, a filler fat composition is described which is obtained by a combination of a component A which is a partially hydrogenated fat with a high content of c18fatty acid, and with component B which is a vegetable oil containing a high proportion of lauric acid. The blend is then subjected to interesterification. As the starting material for component A, any animal fat or vegetable oil may be used as long as it contains at least 50 % of c18 acid. As component B, any vegetable oil which contains lauric acid may be used. Coconut oil and palm kernel oil are satisfactory. This filler fat composition shows a short plastic range, that is, a wide temperature range over which the fat is soft workable and yet is in the solid state. In US 4,834,991, a filler cream containing soybean oil is disclosed which has an improved "get away" property resulting in a rapid burst of flavor when consumed. The filler cream ingredients are mixed, aerated, and cooled simultaneously to obtain a specific gravity of between about 0.85 and about 1.20. The resulting filler cream is utilized as a filler for sandwich cookies or other baked goods. In US 4,753,812, there is disclosed a sandwich cream filling composition comprising a fat matrix consisting essentially of a plastic fat of defined SFI profile and melt point and a hard butter also of defined SFI profile and melt point The composition is characterized as having good stand-up throughout a wide temperature range from about 10 °C to about 32 °C and an imperceptible solids content at temperatures above about 37 °C. The products which are used as a stiffener ingredient in the abovementioned US patent applications, such as non-fat dry milk or soy protein, are effective in increasing plastic and yield values of the filler composition, at higher temperatures. However, these filler fat compositions comprise saturated and/or partially hydrogenated fats. The incorporation of saturated and/or partially hydrogenated fats in food compositions has become a real health issue in recent years. Indeed, both types of fats are directly associated with nonfatal heart attacks and deaths from coronary heart diseases. It is also believed that the amount and type of dietary fat influences the incidence of depression. Further, it has also been reported that the presence of trans-fatty acids, present in partially hydrogenated fats, also increases the risk of getting diabetes and some types of cancer such as breast cancer. Therefore there is a growing concern regarding the use of these saturated or partially hydrogenated oleaginous compositions in a broad range of food compositions, including cream filler compositions. Attempts to reduce fat content and/or caloric content in cream fillings were already undertaken, as shown in the patent literature related to that subject. For instance, in the example of GB 1 458 568, cream fillings are disclosed containing 22 % water, 45 % sugar and 33 % shortening. This shortening contains an emulsifier system allowing the preparation of stable cream fillings containing an important amount of water. The cream filling composition disclosed in EP 827 693 is in a substantially non-emulsified form, and is composed of 10-20 % water, 30-60 % fats, milk protein and sugar. Thereby the fat fraction is characterised by a solid content of >= 8 % at 30°C. However, the presence of too much water in cookie cream fillings can cause the cookies to become soggy and lose crunchiness. In DD 285 469, reduced fat cream fillings are described, composed of an aqueous mixture of sugar and thickener, combined with a fat component. In US 5,939,127, a low fat cream filling is described whereby the fat component is replaced by a fat replacer composition, in combination with glycerol. This fat replacer is an aqueous mixture of an emulsifier and inulin. In US 5,626,903, fat reduction is realised by using an emulsifier system thereby reducing fat content to at least below 16%. An anhydrous reduced fat filler composition is disclosed in US 5,612,078, thereby replacing part of the fat with a polyol (glycerol) and a particular emulsifier. In all the documents cited above, saturated and/or partially hydrogenated oleaginous compositions are part of the formulations. Therefore, it would be highly desirable that substantially all saturated and/or partially hydrogenated fats are replaced by unsaturated or poly-unsaturated fats or oils. In addition, the solutions of the prior art all have one or more shortcomings with regard to nutritional, organoleptic, functional, economical and/or process-related characteristics. Therefore, the purpose of the invention is to provide cream filler compositions, substantially free of saturated fats and free of partially hydrogenated fats, containing almost exclusively natural vegetable oils having high contents of unsaturated and/or poly-unsaturated fatty acids, but in which the cream fillings should retain consistency at room temperature and provide good organoleptic properties when consumed; the fillings must have good whipping and creaming properties, so as to impart to the filler cream a light and consistent texture upon whipping and aeration; the cream filler composition should have good shelf storage properties. With substantially free of saturated fats and free of partially hydrogenated fats is meant: - a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction; and - a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction, With natural vegetable oils there is meant vegetable oils that were not submitted to partial hydrogenation, thereby showing a SFI (solid fat index) of about 0 (zero) at room temperature (25 ° C). The purpose of the invention is achieved by providing a cream filler composition which comprises: - a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction, - a powder sweetener composition, - and a wheat gluten fraction having an increase gliadin content compared to the natural gliadin content in wheat gluten. In a preferred cream filler composition according to the invention, the cream filler composition comprises: from 25 - 60 % by weight of a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction; from 40 to 70 % by weight of a powder sweetener composition; and from 1 to 15 % by weight of a wheat gluten fraction having an increased gliadin content compared to the natural gliadin content of wheat gluten. More preferably, the cream filler composition according to the invention comprises: from 30 - 50 % by weight of a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction; from 45 to 65 % by weight of a powder sweetener composition; and from 3 to 12 % by weight of a wheat gluten fraction having an increased gliadin content compared to the natural gliadin content of wheat gluten. In an advantageous embodiment of a cream filler composition according to the invention, the powder sweetener composition comprises one or more carbohydrate-based sweeteners. In another advantageous embodiment of a cream filler composition according to the invention, the powder sweetener composition comprises one or more high intensity sweeteners. In still another advantageous embodiment of a cream filler composition according to the invention, the powder sweetener composition comprises a combination of one or more carbohydrate-based sweeteners and high intensity sweeteners. The wheat gluten fraction of the cream filler composition according to the invention comprises preferably a gliadin-rich fraction wherein the gliadin/glutenin ratio is at least 2, more preferably at least 2,5 and most preferably at least 3, as determined by means of the solvent fractionation method. In a preferred embodiment of a cream filler composition according to the invention, the wheat gluten fraction is obtained by means of the following method: the gluten are dispersed continuously or not in water up to a dry substance varying between 5 and 30 %, by which • the pH of the dispersion is monitored between 4,4 and 4,8, and • the gluten - water mixture is submitted to shearing actions, through which the dispersion, continuously or not, can be fractionated in gliadin- and glutenin-rich fractions, by which a single gliadin-rich fraction with a gliadin/glutenin ratio of at least 2,5 is obtained, and a single glutenin-rich fraction with a gliadin/glutenin ratio of less than 0,8 is obtained. In a first preferred method for preparing a cream filler composition according to the invention, the cream filler composition is prepared batch-wise. In one possibility, the batch-wise preparation is performed by first mixing the gliadin-rich fraction with the powder sweetener and then adding oil while mixing continuously until a homogeneous viscous cream is obtained. In another possibility, the batch-wise preparation is performed by first mixing the gliadin-rich fraction with the oil and then adding the powder sweetener while mixing until homogenenous. In a second preferred method for preparing a cream filler composition according to the invention, the cream filler composition is prepared in a continuous way. The continuous preparation is for instance performed by continuously dosing the powder sweetener, the gliadin-rich fraction and the oil in a continuous mixing unit Further distinctive features and characteristics will be clarified in the following description and the following examples, which should be considered as being no restriction in the general scope of the invention as that appears from the above description and from the claims at the end of this text. The cream filling composition of the invention is characterised in that it comprises: - a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, and a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction, - a powder sweetener composition, - and a wheat gluten fraction having an increased gliadin content, compared to the natural gliadin content in wheat gluten. The powder sweetener composition typically comprises one or more carbohydrate-based sweeteners, but may also contain high intensity sweeteners. The most important vegetable oils that can be used in these cream filling compositions are a,o.? (but not limited to) soybean oil, maize oil, sunflower oil, rapeseed oil, canola oil, peanut oil, cottonseed oil, olive oil, and saffiower oil. The powdered sweetener composition may comprise a powder of a.o. sucrose, dextrose, fructose, maltitol, sorbitol, isomaltitol, lactitol, xylitol, erythritol, mannitol, polydextrose, tagatose, maltodextrine, hydrogenated or non-hydrogenated starch hydrolysates, hydrogenated maltodextrine or a mixture of two or more of these products. These carbohydrate-based sweeteners can further be combined with a high intensity sweetener such as e.g. aspartame, acesulfam K, saccharin, cyclamate, alitame, sucralose, stevioside, neohesperidine, etc. The wheat gluten fraction typically comprises a gliadin-rich fraction wherein the gliadin/glutenin ratio is at least 2, as determined by means of the "solvent fractionation" method as disclosed in WO 03/013266 (see fig. 2). More preferably the gliadin/glutenin ratio in the wheat gluten fraction is at least 2,5 and most preferably at least 3. This solvent fractionation method uses the different solubility characteristics of the wheat protein classes in different solvents to separate gliadins, glutenins and albumins and globulins. Distribution of protein in the different solvents is quantified by Kjeldahl analysis. Equipment ■ Polypropylene centrifuge tubes of 40 ml ? Centrifuge (able to spin at 15000 g) ? Analytical balance ? 0.5MNaCl ? 1.5% SDS solution ? 95% Ethanol ■ Incubation chamber at 10°C ? Kjeldahl apparatus ? Kjeldahl tablets ? For 300 ml tubes: type CT (5g K2S04 + 0.15g CuS04.5H20 + 0.15g Ti02). ? For 100 ml tubes: type CTQ (l,5g K2S04 + 0.045g CuS04.5H20 + 0.045g Ti02). Procedure The extraction procedure is outlined in figure 2. All extractions are for 30 min at room temperature. ? Weigh a sample in a PP-centrifuge tube of +/- 40 ml. For flour 2.000 g, for gluten 200 mg, for process samples the amount corresponding to +/- 150-160 mg of protein. ? Add 20 ml 0.5M NaCl. Stir for 30 min at room temperature (RT). After extraction, centrifuge at 500 g for 15 min at RT. Carefully decant the supernatant in a clean PP-tube. Add another 20 ml of 0.5M NaCl to the residue (precipitate A) and repeat the extraction and centrifugation. Combine this second supernatant with the first and then centrifuge at 15000 g for 15 min at RT. The supernatant contains the albumins and globulins. ? To the residue (precipitate B) is added 6 ml 1.5% SDS solution. The mixture is homogenised and combined with precipitate A. Extraction is for 30 min at RT. 14 ml of absolute ethanol is then added dropwise and the mixture is stirred for another 30 min at RT. After centrifugation at RT for 15 min at 500 g9 a precipitate and supernatant 3 are obtained. This complete procedure is repeated on the obtained precipitate, yielding precipitate C and supernatant 4. ? Supernatants 3 and 4 are combined in a small beaker and kept in an incubation chamber at 10 °C for 60 min. A fine precipitate forms and this mixture is then poured into the centrifuge tube containing precipitate C. The mixture is immediately transferred to the centrifuge and spun at J 5000 g for 15 min at 10 °C. This yields a supernatant containing gliadins and a precipitate containing glutenins. Kjeldahl determination The solutions containing the albumins/globulins and gliadins (+/- 40 ml) are quantitatively transferred to destruction flasks (750 ml). A tablet, 14 ml of concentrated sulphuric acid and 3 drops of octanol (antifoam) are added and samples are destroyed for 90min (untill clear). The concentrate (about 15 ml) is transferred to a Kjeldahl tube and nitrogen is determined as described in Amylum method 0701. The glutenin fraction is freeze dried and nitrogen content is determined as in dry products. Results are reported as the amount of protein found in the different classes and expressed as a percentage of the recovered protein. Thereby it is desirable that the above cited wheat gluten fractions are obtained by means of a process as disclosed in WO 03/013266, i.e. the gluten are dispersed continuously or not in water up to a dry substance varying between 5 and 30 %, by which • the pH of the dispersion is monitored between 4,4 and 4,8, and • the gluten - water mixture is submitted to shearing actions, through which the dispersion, continuously or not, can be fractionated in gliadin- and glutenin-rich fractions, by which a single gliadin-rich fraction with a gliadin/glutenin ratio of at least 2,5 is obtained, and a single glutenin-rich fraction with a gliadin/glutenin ratio of less than 0,8 is obtained. In an advantageous aspect of the invention the cream filler composition is characterised in that it comprises: - from 25-60% by weight of a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, and a saturated fat fraction representing 0-5% by weight of the total lipid fraction - from 40 to 70% by weight of a powder sweetener composition, - and from 1 to 15% by weight of a wheat gluten fraction having an increased gliadine content, compared to the natural gliadine content of wheat gluten. In a more advantageous aspect of the invention the cream filler composition is characterised in that it comprises: - from 30-50% by weight of a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, and a saturated fat fraction representing 0-5% by weight of the total lipid fraction, - from 45 to 65% by weight of a powder sweetener composition, and from 3 to 12% by weight of a wheat gluten fraction having an increased gliadin content, compared to the natural gliadin content of wheat gluten. The products according to the invention can be prepared batch-wise or in a continuous way. In a batch-wise process, the gliadin-rich fraction can be first mixed with the sweetener powder. Then the oil can be added while mixing continuously until a homogeneous viscous cream, is obtained. Alternatively, the gliadin-rich fraction is first mixed with the oil, and then the sweetener compound is added, while mixing until homogeneous. A continuous method comprises e.g. continuous dosing of sweetener, gliadin-rich product and oil into a continuous mixing unit, (e.g., but not limited to Lodige-type, VOMM-type, or Denissen-type mixers) The thus obtained compositions do show a number of mechanical properties making them very suitable as cream fillers for sandwich cookies. Important properties are consistency at room temperature and "melt" behaviour at body temperature, in combination with a light and consistent texture. Furthermore, shelf stability is also very important. Another advantage of the process according to the invention relates to the fact that, in contrast to the existing state of the art processes, such as described in US 4,753,812 and US 4,834,991 (as mentioned in documents of the state of the art), there is no longer a need for a heating and cooling/ciystallisation step to obtain cream filler compositions for e.g. sandwich cookies or layered wafer cookies. The invention also pertains to food compositions containing the cream filler of the invention. Typical representatives are e.g. sandwich cookies or layered wafer cookies. EXAMPLES: Example 1-2: in the following examples, cream filler compositions according to the invention are compared with compositions containing protein ingredients other than the high gliadin wheat gluten fractions (hereafter called gliadins). Cream filler functional properties such as consistency of texture and storage stability are determined respectively, as the compression force measured by means of a texture analyser, and oil leaching in function of time. In the following table la and lb, several compositions are described containing different types of proteins. Table la discloses compositions with standard formulations. These standard formulations are based on two literature sources: - information of Kraft General Foods, for instance the Oreo Cookies; - booklet "Secondary processing in biscuit manufacturing" by Duncan Manley, date 1998, chapter 6 "Cream sandwiching". On page 43 is stated that it is usual to use a recipe which has around 30 % fat in the cream, but levels as low as 20 % and as high as 45 % will be found. Table lb is concerned with analogous compositions in comparison with these Oreo Cookies, but containing the gliadin enriched fraction according to the invention. Quantities mentioned are in parts by weight. The cream filler compositions are prepared as follows: Icing sugar and protein ingredients are first dry mixed in a Kenwood mixer equipped with a K beater. Then all the oil is added at once. During the first 15 seconds mixing is performed at low speed. During the following 3 minutes the ingredients are mixed at maximum speed. The thus obtained cream filler compositions are then compared with commercial cream filler compositions containing saturated and/or partially hydrogenated fats/oils. The commercial cream fillers were obtained by recovering the cream filler material present between the cookies of three commercial products: Oreo's, Prince-LU and Frou Frou wafers. Following tests were performed in order to compare these different formulations: Texture analysis: The cream fillers prepared are put in a small cup having the dimensions of 12 mm high and a diameter of 5,4 cm. The sample is conditioned at least for one hour at the temperature at which it is measured (room temperature). A cylindric probe of 5 mm diameter is displaced at a speed of 1 mm/sec over a distance of 5 mm into the cream filler. Thereby the compression force (in g) is measured as an indicative value for texture consistency. Oil retention stability: On a silicon paper a layer of cream filler is spread, the height being 2 mm. With a sharp edged cylinder of 32,2 mm diameter a disc is cut out and placed in the centre of a synergy paper. (Half hour Blotter test paper, Bridge & Company, printers 8c stationers)(see fig. 1) Then the paper containing the cream filler is stored at 25°C and the spreading of oil is measured at specific times. The spreading of oil is determined as the average value of spreading in the four directions. Oil spreading is thereby inversely related to storage stability. Table 2 comprises data on compression force of the cream fillers as well as data on oil spreading in function of time. Values of commercial fillings are compared with the product compositions of table la and lb. (1): No compression value could be determined. The comparative examples 1 to 4, containing soy isolate, caseinate, and wheat gluten or no protein, do not provide a consistent texture. They are not storage-stable over longer periods as show by the oil retention test, also. The examples 1 and 2 according to the invention provide stable consistent cream filler compositions, this on the basis of liquid, unsaturated oils. Examples 3-7: In this series of examples according to the invention, a number of formulations, showing a variable sugar, oil and protein content, are tested. The composition of the cream fillings is provided in table 3 while consistency values and storage stability values are disclosed in table 4. From the above it is clear that different consistencies can be obtained as a function of the filler composition. Also, storage stability comparable to the stability of the commercial formulations could be obtained using the formulations of the invention. CLAIMS 1. Cream filler composition, characterised in that it comprises: - a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction, - a powder sweetener composition, - and a wheat gluten fraction having an increase gliadin content compared to the natural gliadin content in wheat gluten. 2. Cream filler composition according to claim 1, characterised in that it comprises: from 25 - 60 % by weight of a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction; from 40 to 70 % by weight of a powder sweetener composition; and from 1 to 15 % by weight of a wheat gluten fraction having an increased gliadin content compared to the natural gliadin content of wheat gluten. 3. Cream filler composition according to claim 2, characterised in that it comprises: from 30 - 50 % by weight of a lipid fraction composed of a vegetable oil having a SFI of 0 at room temperature, a saturated fat fraction representing between 0 % and 5 % w/w of the total lipid fraction and a partially hydrogenated fat fraction representing between 0 % and 1 % w/w of the total lipid fraction; from 45 to 65 % by weight of a powder sweetener composition; and from 3 to 12 % by weight of a wheat gluten fraction having an increased gliadin content compared to the natural gliadin content of wheat gluten. 4. Cream filler composition according to any one of the preceding claims, characterised in that the powder sweetener composition comprises one or more carbohydrate-based sweeteners. 5. Cream filler composition according to any one of the claims 1 to 3, characterised in that the powder sweetener composition comprises one or more high intensity sweeteners. 6. Cream filler composition according to any one of the claims 1 to 3, characterised in that the powder sweetener composition comprises a combination of one or more carbohydrate-based sweeteners and high intensity sweeteners. 7. Cream filler composition according to any one of the claims 1 to 6, characterised in that the wheat gluten fraction comprises a gliadin-rich fraction wherein the gliadin/glutenin ratio is at least 2, as determined by means of the solvent fractionation method. Cream filler composition according to claim 7, characterised in that the wheat gluten fraction comprises a gliadin-rich fraction wherein the gliadin/glutenin ratio is at least 2,5 as determined by means of the solvent fractionation method. Cream filler composition according to claim 8, characterised in that the wheat gluten fraction comprises a gliadin-rich fraction wherein the gliadin/glutenin ratio is at least 3, as determined by means of the solvent fractionation method, 10. Cream filler composition according to any one of claims 1 to 9, characterised in that the wheat gluten fraction is obtained by means of the following method: the gluten are dispersed continuously or not in water up to a dry substance varying between 5 and 30 %, by which • the pH of the dispersion is monitored between 4,4 and 4,8, and • the gluten - water mixture is submitted to shearing actions, through which the dispersion, continuously or not, can be fractionated in gliadin- and glutenin-rich fractions, by which a single gliadin-rich fraction with a gliadin/glutenin ratio of at least 2,5 is obtained, and a single ghxtenin-rich fraction with a gliadin/glutenin ratio of less than 0,8 is obtained. 11. Method for preparing a cream filler composition according to any one of the preceding claims, characterised in that the cream filler composition is prepared batch-wise. 12. Method according to claim 11, characterised in that the batch-wise preparation is performed by first mixing the gliadin-rich fraction with the powder sweetener and then adding oil while mixing continuously until a homogeneous viscous cream is obtained. 13. Method according to claim 11, characterised in that the batch-wise preparation is performed by first mixing the gliadin-rich fraction with the oil and then adding the powder sweetener while mixing until homogenenous. 14. Method for preparing a cream filler composition according to any one of the claims 1 to 10, characterised in that the cream filler composition is prepared in a continuous way. 15. Method according to claim 14, characterised in that the continuous preparation is performed by continuously dosing the powder sweetener, the gliadin-rich fraction and the oil in a continuous mixing unit 16. Food compositions comprising a cream filler composition according to any one of the claims 1 to 10. Dated this 30 day of June 2006

Documents:

2399-CHENP-2006 AMENDED PAGES OF SPECIFICATION 25-07-2011.pdf

2399-CHENP-2006 AMENDED CLAIMS 25-07-2011.pdf

2399-chenp-2006 form-1 25-07-2011.pdf

2399-chenp-2006 form-3 25-07-2011.pdf

2399-CHENP-2006 OTHER PATENT DOCUMENT 1 25-07-2011.pdf

2399-chenp-2006 other patent document 2 25-07-2011.pdf

2399-CHENP-2006 POWER OF ATTORNEY 25-07-2011.pdf

2399-CHENP-2006 AMENDED PAGES OF SPECIFICATION 09-02-2012.pdf

2399-CHENP-2006 AMENDED CLAIMS 09-02-2012.pdf

2399-CHENP-2006 EXAMINATION REPORT REPLY RECEIVED 25-07-2011.pdf

2399-CHENP-2006 FORM-3 09-02-2012.pdf

2399-CHENP-2006 OTHER PATENT DOCUMENT 09-02-2012.pdf

2399-CHENP-2006 CORRESPONDENCE OTHERS 15-02-2012.pdf

2399-CHENP-2006 CORRESPONDENCE OTHERS 27-02-2012.pdf

2399-CHENP-2006 CORRESPONDENCE OTHERS 09-02-2012.pdf

2399-CHENP-2006 CORRESPONDENCE OTHERS 11-03-2011.pdf

2399-CHENP-2006 FORM-3 27-02-2012.pdf

2399-chenp-2006-abstract.pdf

2399-chenp-2006-claims.pdf

2399-chenp-2006-correspondnece-others.pdf

2399-chenp-2006-description(complete).pdf

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2399-chenp-2006-form 1.pdf

2399-chenp-2006-form 3.pdf

2399-chenp-2006-form 5.pdf

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