Title of Invention

"IMPROVED TISSUE CHAMBER ASSEMBLY FOR ISOLATED ORGAN BATH APPARATUS"

Abstract This invention relates to an improved tissue chamber assembly for solated organ bath apparatus adaptable to evaluate the effects of drugs and chemicals on the tissues isolated from freshly sacrificed animals, comprising of a capacitatively adjustable multipart tissue chamber (2) formed out of non-reactive cylindrical plastic and being imprinted with graduations; a tissue holder cum aerator (1) molded from slender stainless steel in registration with the configuration of the tissue chamber (2); and, a three-way connector (3) formed of non-reactive plastic for fixing the tissue chamber including the warming coil (6).
Full Text FIELD OF INVENTION
The invention generally relates to an isolated organ bath apparatus, which is adapted for evaluating the effects of drugs and chemicals on the tissues isolated from freshly sacrificed animals. More particularly, the invention relates to an improved tissue chamber device for an isolated organ bath apparatus.
BACKGROUND OF THE INVENTION
Isolated organ bath apparatus is extensively used for testing of tissues of animals. Such apparatus in the prior art consists of the following features:-
A Tissue chamber made of glass in single part and houses an isolated tissue perfused with physiological salt solution (PSS). Drugs and chemicals are added to the chamber for testing their effects on the tissue behaviour. A Tissue holder cum aerator tube-which is a hollow cylindrical glass tube is placed into the tissue chamber, and which serves two functions. It holds the tissue in position inside the tissue chamber while aerating the PSS held inside the tissue chamber. The organ bath apparatus further comprises of a water jacket containing water maintained at a predetermined temperature, and surrounding the tissue chamber, a thermostat is provided adjacent to the tissue chamber to control the water temperature, a heating element is provided to heat the water, a stirrer arranged above the heater to achieve uniformity of water temperature, an aerator distantly disposed being flowably connected to pump atmospheric air into the tissue chamber; a force transducer disposed exterior to the tissue chamber for picking-up the data relating to mechanical changes in the tissue, and converting the physiological data into electrical signals for transmitting to an
operably connected physiograph. A warming coil made of glass tube serves to warm the PSS delivered from a storage being flowably connected to the tissue chamber.
However, the disadvantages of the prior art apparatus are that the tissue chambers are of fixed size which results in wastage of PSS and the drugs being inputted for testing, in particular when small tissues are under study. Again, such tissue chambers can not be adapted for various sizes of tissues under study. Further the tissue chambers are not graduated, which may lead to human inaccuracies in measurement because the exact fluid content has to be calculated by the individual performing the tests.
In addition, as the tissue chambers being made of glass, extra care must be taken in handling those. Frequent breakage could disrupt an experiment.
Moreover, due to fixed nature of the tissue chamber, it is difficult to clean it off the drugs or chemicals used during the experiment. This could lead to contamination of subsequent experiments.
The tissue holder with aerator tube constitutes of glass and cannot be adapted to different sizes of tissue chambers.
However the prior art tissue chamber device for the isolated organ bath apparatuses have further disadvantages in that they are generally not replaceable being costly. The running cost including the maintenance cost of such apparatus is quite high, apart from the fact that the core components namely the tissue chamber device is fragile being made of glass.
Additionally, the core components are not capacitatively adjustable which leads to frequent change of the components to suit the requirement of testing. The consumption of chemicals and drugs is higher being manually operated based on human judgement.
OBJECTS OF THE INVENTION
It is an object of the present invention to propose an improved tissue chamber device for isolated organ bath apparatus, which eliminates the disadvantages of the prior art.
Another object of the invention is to propose an improved tissue chamber device for isolated organ bath apparatus, which reduces consumption of precious chemicals and thereby reduce the operational cost of the apparatus.
A further object of the invention is to propose an improved issue chamber device for isolated organ bath apparatus, which is configured as capacitatively adjustable according to the demands.
Yet another object of the invention is to propose an improved tissue chamber device for isolated organ bath apparatus, which reduces the propensity of human error.
A still further object of the invention is to propose an improved tissue chamber device for isolated organ bath apparatus, which is easy to maintain and less expensive to manufacture.SUMMARY OF THE INVENTION
Accordingly there is provided an improved tissue chamber assembly for isolated organ bath apparatus adaptable to evaluate the effects of drugs and chemicals on the tissues isolated from freshly sacrificed animals, comprising of:-
- a capacitatively adjustable multipart tissue chamber formed out of
non-reactive cylindrical plastic and being imprinted with
graduations;
a tissue holder cum aerator molded from slender stainless steel in registration with the configuration of the tissue chamber; and,
- a three-way connector formed of non-reactive plastic for fixing the
tissue chamber including the warming coil.
The invention has incorporated improvement in the core unit of the apparatus known in the prior art. The core unit namely, the tissue chamber has been configured in multipart introducing a three-way connector. The tissue chamber is now made of non-reactive transparent plastic and is also provided with imprinted graduation. The three-way connector is made of non-reactive transparent plastic having three openings. The tissue holder-cum-aerator tube is made of a slender stainless steel tube. It is bent at right angles and has a hook like forming at one end to hold a tissue.
The invention will now be described with the help of the following accompanying drawings.
BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
Fig. 1 shows an isolated organ bath apparatus according to the invention. Fig. 2 shows the constituent components of a tissue chamber device comprising a tissue holder-cum-aerator tube, a tissue and a three-way connector according to the invention.
DETAILED DESCRIPTION OF A PREFERRED EMBODIMENT OF THE INVENTION
As shown in Fig. 1 an organ bath casing (4) of the apparatus constitutes a transparent perspex case which has water filled upto a desired height. The water level (14) is maintained such that a tissue chamber (2) when disposed inside the casing (4) is submerged upto a desired level. A tissue holder-cum-aerator tube (1) is placed with its one end inside said tissue chamber (2) where a tissue (5) being tested is held in position by the tube (1). The other end of said tube (1) is connected at distal end of an aerator (12) which pumps atmospheric air or oxygen through the tube (1) into the tissue chamber (2).
The bottom opening of the tissue chamber (2) is connected to a top end (15) of a three-way connector (3). A warming coil (6) is placed on a side opening (16) of the connector (3) to ensure desired warming of PSS entering the tissue chamber (2). The side opening of the warming coil (6) is connected to storage (13) for the physiological salt solution (PSS), which is placed at a higher level than the organ bath casing (4) to facilitate gravity flow of PSS for perfusion of the tissue (5) within the tissue chamber (2). A bottom opening (17) of said connector (3) is attached to drainage. A thermostat (7) is placed inside the organ bath casing (4) to ensure maintenance of the bath temperature at a desired temperature. A heater (8) disposed in the bath casing to warm the delivered water. A stirrer (9) interposed between the thermostat (7) and the heater (8) to distribute the heat uniformly and maintain a predefined temperature inside the bath casing (4).
A force transducer (10), operably connected to the tissue chamber (2) which transmits electrical signals corresponding to the physio-mechanical changes in the treated tissue (5). The outputted electrical signals from the force transducer (10) are fed to a physiograph (11) which produces a graphical representation of the physiological changes occurring in said tissue (5) due to the effect of the drugs and chemicals.
The tissue chamber (2) in accordance with the invention is available in several sizes. The right size of the said chamber depends on the size of the tissue (5) to be tested and the requisite quantity of the PSS that the chamber must be able to hold for proper performance of the experiment.
The tissue chamber device of the improved isolated organ bath apparatus is configured in multipart to make the device capacitatively adjustable. The device is divided into three parts viz. tissue chamber (2), tissue holder-cum-aerator (1) and a three way connector (3). The tissue chamber (2) is made up of cylindrical plastic and is graduated. The tissue holder-cum-aerator (1) is made up of stainless steel hollow tube. It is molded in correspondance with the chamber (2).
The three way connector (3) being adaptable in registration with the configuration of the tissue chamber and is used to fix the tissue chamber (2) and the warming coil (6) with the organ bath base (4). The third opening (17) of the connector (3) is used for draining the waste fluid from the tissue chamber (2).
The utility of the invention can be construed from the example given below:
Chemical required : 2-chloroadenosine (Sigma, USA) and salts such as NaCI, KCI, NaCHO3, NaH2PO4, MgCl2 and CaCI2 and glucose to make Krebs solution.
Procedure : The tissue (5) is isolated from a freshly sacrificed rat and mounted in the isolated organ bath assembly. The tissue (S) is equilibrated for 1 hr, during which the tissue chamber (2) (4-5 ml capacity), fluid is replaced every 15 min. The spontaneous contractions are recorded, thereafter the drug 2-chbroadenosine (2-CAD) is added and observed for next 15-20 min. This experiment was repeated with four different doses of 2-CAD.
Results: The results show that the drug 2-CAD reduces the frequency as well as amplitude of the rhythmic contractions of the rat portal vein significantly.
To reach a concrete conclusion, this experiment/parameter has to done at least 6-8 times with consistent results.
If the same testing is was to be done by the existing isolated organ bath apparatus, the above mentioned chemicals would have a consumption 4-5 times more than that required by the improved apparatus. This gives a saving of 75-80% of total experimental cost. The unbreakable material used for configuration of the core components of the improved apparatus make it safe and less expensive. Results of the evaluation are 100% reproducble, the propensity of human error is eliminated. As the core components are capacitatively adjustable according to requirement, the cost, time and accuracy remain under control.
The present invention substantially reduces consumption of precious chemicals, which are quite often imported from abroad, and thereby reduce the cost of performing such a test. The tissue chamber is flexbie and adjustable to the
demands of the experiment. The inventive features reduces human error. The possibility of breakage of the tissue chamber during handling is elim inated. The invention discloses a disposable assembly, especialy an interchangeable tissue chamber which is cheap and cost-effective.










WE CLAIM;
1. An improved tissue chamber assembly for isolated organ bath
apparatus adaptable to evaluate the effects of drugs and
chemicals on the tissues isolated from freshly sacrificed animals,
comprising of:-
- a capacitatively adjustable multipart tissue chamber (2) formed out
of non-reactive cylindrical plastic and being imprinted with
graduations;
a tissue holder cum aerator (1) molded from slender stainless steel in registration with the configuration of the tissue chamber (2); and,
- a three-way connector (3) formed of non-reactive plastic for fixing
the tissue chamber including the warming coil (6).
2. The device as claimed in claim 1, wherein a proximal end of the
tissue holder-cum-aerator (1) is interposed in the tissue chamber
(2) with its distal end projecting outwardly for flowably connecting
an aerator (12).
3. The device as claimed in claim 1, wherein a tissue (5) under testing is suspendendly held on the tissue holder-cum-aerator (1).

Documents:

3283-DEL-2005-Abstract-(10-12-2010).pdf

3283-del-2005-abstract.pdf

3283-DEL-2005-Claims-(10-12-2010).pdf

3283-del-2005-claims.pdf

3283-DEL-2005-Correspondence-Others-(10-12-2010).pdf

3283-del-2005-correspondence-others.pdf

3283-del-2005-correspondence-po.pdf

3283-DEL-2005-Description (Complete)-(10-12-2010).pdf

3283-del-2005-description (complete).pdf

3283-del-2005-drawings.pdf

3283-DEL-2005-Form-1-(10-12-2010).pdf

3283-del-2005-form-1.pdf

3283-del-2005-form-18.pdf

3283-del-2005-form-2.pdf

3283-del-2005-form-3.pdf

3283-DEL-2005-GPA-(10-12-2010).pdf

3283-DEL-2005-Petition 137-(10-12-2010).pdf


Patent Number 249639
Indian Patent Application Number 3283/DEL/2005
PG Journal Number 44/2011
Publication Date 04-Nov-2011
Grant Date 01-Nov-2011
Date of Filing 06-Dec-2005
Name of Patentee CHOUDHARY SARWAN KUMAR KRISHI VISHVAVIDYALAYA
Applicant Address VAVIDYALAYA, PALAMPUR, 176062 HIMACHAL PRADESH, INDIA.
Inventors:
# Inventor's Name Inventor's Address
1 RAVINDRA SUDHAKAR TELANG NATIONAL OF DEPARTMENT OF PHARMACOLOGY & TOXICOLOGY, COLLEGE OF VETERINARY & ANIMAL SCIENCES, CSK HIMACHAL PRADESH AGRICULTURAL UNIVERSITY, PALAMPUR-176062, HP, INDIA.
PCT International Classification Number D21F 3/08
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA