Title of Invention

DEVELOPMENT OF HARBAL NUTRITIOUS CHOCAEATE AND ITS PROCESSING

Abstract The present invention provides a herbal nutritious composition of extracts of plants Tinospora cordifolia, Glycyrrhyza glabra, Adhatoda vasica, Madhuca indica and Cassia occidentalis. The extracts of these plants are mxed with additives. The plant extract comprises 20-40 % and additives 60-80%. The solid or semisolid nutritious composition is rich in protein/mineral fortified with standardized herbs with proven pharmacological activities such as anti-oxidant, anti stress and adaptogenic property and relief the chronic cough & cold. The novel herbal nutritious composition comprises of decoction of Tinospora cordifolia, Glycyrrhyza glabra, Adhatoda vasica, and paste of Madhuca indica with roasted seeds of Cassia occidentalis. In a battery of tests like hypoxia and swimming endurance the synergistic effect of the composition showed 90% potency confirms the adaptogenic activity
Full Text FIELD OF INVENTION
The present invention relates to a herbal nutritious composition. The novel herbal composition(s) provides proven pharmacological activities such as anti-oxidant, anti stress, adaptogenic and immune enhancing property. The invention also provides a method for preparation of said composition. BACKGROUND AND PRIOR ART OF THE INVENTION
The origins of chocolate can be traced back to the ancient Maya and Aztec civilizations in Central America, who first enjoyed 'chocolate; a much-prized spicy drink made from roasted cocoa beans. Chocolate was exclusively for drinking until the early Victorian era, when a technique for making solid 'eating' chocolate was devised. Throughout its history, whether as a cocoa, a drinking chocolate beverage or confectionery treat, chocolate has been a much sought after food.
As conventional chocolate is assumed to be a causative factor of to migraines, Acne and pimples, diabetes and obesity and also considered as a luxury food where as Cocoa beans contain a type of antioxidant that may have a role in the prevention of certain diseases. The catechins found in cocoa beans aid resistance against degenerative diseases such as cancer and heart disease. In this context the thought has aroused of providing a chocolate type of edible substance which can provide positive effects with lesser or no side effect, for it the proper blending of modern knowledge with traditional Indian system of medicine, preparing medicine in the form of food which is therapeutically effective & economical too.
OBJECTS OF THE INVENTION
The main object of the present invention to provide a novel herbal nutritious chocolate and it's processing.
SUMMARY OF THE INVENTION
Accordingly the present invention provides a herbal nutritious composition useful in the treatment of anti fatigue, anti stress agent and provides vitality and energy.
The novel herbal nutritious composition comprises of decoction of Tinospora cordifolia, Glycyrrhyza glabra, Adhatoda vasica, and paste of Madhuca indica with roasted seeds of Cassia occidentalis. In a battery of tests like hypoxia and swimming endurance the synergistic effect of the composition showed 90% potency confirms the adaptogenic activity. The detail description of the plants used in the invention are having the following properties as reported.
Tinospora cordifolia Family: Menispermaceae
Botanical Descriptions: A large glabrous climbing shrub. Stems rather succulent with long filiform, aerial roots arising from branches. Bark; warty, papery thin, creamy white or grey brown. Peels off easily. Wood, soft, perforated. Leaves; membranous, cordate with broad sinus. Pointed at the tip. Flowers; unisexual and greenish, in long clusters. Seeds; curved. Drupes; ovoid, succulent, lustrous, red, pea sized. Fruits; fleshy, one seeded. Flowers during the summer and fruits during the winter. It is found throughout tropical India, ascending to an altitude of 300m.
Medicinal Uses: Useful in bilious fever, rheumatism, general debility, seminal weakness, splenic diseases and urinary affections. Fresh plant is considered more efficacious. It is mostly used for preparing a kind of starch knovm as Guduchi satva or Sat giloe.
Phytochemistry: Sesquiterpene tinocordifolin, sesquiterpene glucoside tinocordifoiioside, tinosponone, tinocordioside, cordioside, fiaranoid diterpenes, a new clerodane furano-diterpene viz. columbin, tinosporaside, an immunologically active arabinogalactan, two phytoecdyyones viz., ecdysterone and makisterone and several glycosides isolated as polyacetates. Other alkaloids viz., jatrorrhizine, palmatine, berberine, tembeterine, phenylpropene disaccharides cordifolioside A, B and C, choline, tinosporic acid, tinosporal, tinosporon, 20-P-hydroxyecdysone, palmatoside C and F, cordifolisides D and E, diterpenoid fiiranolactones.
Pharmacology; The water and ethanolic extract inhibited the cyclophosphamide induced immunosupression. Aqueous extract of the stem showed anti-inflammatory, analgestic and antipyretic properties in rats. In clinical studies, it also showed immunosuppressive
effect in obstructive jaundice patients, antioxidant activity and amelioration of cylcophosphamide-induced toxicity.
Glycyrrhyza glabra Family: Papilionaceae.Fabaceae.
Botanical description: A perennial herbs and under shrubs distributed in the sub -tropical and warm temperate regions of the world, chiefly in the Mediterranean countries and china. The drug is imported into India on a considerable scale from Asia Minor, Iraq, Persia and other Central Asian countries.
Phytochemistry:- Glycyrrhizin, Glycyrrhizic acid, and glycyrrhetinic acid , the characterstic sweet taste is due to Glycyrrhizin (2-14%).
Pharmacology: Rhizomes and roots tonic, expectorant, demulcent, and laxative, used for allaying coughs and catarrhal affections, and irritable conditions of the membranes of the urinary organs.
Madhuca indica (Koenig) Gmelin Family: Sapotaceae
Botanical description: A medium sized to large deciduous tree, leaves clustered near the ends of stout branches 5-25 cm long and 3.5- 12 cm wide, apex shortly acuminate, base rounded to cuneate; petioles 2-4 cm long; stipules 0.6-1 cm long, subulate, densely pubescent. Flowers numerous, scented, clustered on leafless shoots; fruits ovoid or subglobose, 2.5-5 cm long, greenish turning reddish- yellow or orange when ripe, fleshy; seeds 1-4, brown or ochre, oblong - ellipsoid, shiny, 2.5 - 3.8 cm long and 1.5 cm wide. Flowers appear with new leaves from February to April and fruits ripen from May to August in northern and central India.
Pharmacology: The seed oil has emollient properties and is useful for external application in treating skin diseases, rheumatism and headache. It is also laxative and used to treat chronic constipation, piles and haemorrhoids and is sometimes used as an emetic and galactagogue. The ground flowers are applied as a paste to relieve aching muscles. The bark is used for treating rheumatism, ulcers, itches, bleeding and spongy
gums, tonsillitis and diabetes mellitus. The roots are applied externally to promote healing of ulcers.
Cassia occidentalis (L) Family: Caesalpiniaceae
Botanical description: An erect fetid, woody herb or undershrub, 60-150 cm tall, with a smooth, purplish or green stem. Leaves alternate, pinnate, 15-20 cm long, stipulate with a sessile dark brown gland near the base of the petiole; leaflets 3-5 pairs, opposite, short-stalked, membranous, ovate or lanceolate, 3-9 cm long and 1.5-4 cm wide, base rounded, apex acute or attenuate, glabrous above glaucous beneath. Flowers yellow, in short, few flowered axillary or terminal recemes. Flowers and fruits throughout the year depending on locality, but mainly between August and January in central India.
Phytochemistry: The seeds contain tannic acid, mucilage (36%), fatty acid (2.56%), emodin and a toxalbumin. Chrysarobin (methyl - di hydroxy anthranol. M. P. 203-4°C) has been isolated from the benzene extract of the seeds. The fatty oil contains: saturated fatty acids 19.7; linoleic acid, 31.4; oleic acid 30.7, linolenic acid, 6.3;volatile constituents, 0.7; un sapon matter 7.4 %. Pharmacology: All parts of the plant have similar medicinal properties and are considered purgative, tonic, febrifugal, expectorant and diuretic. The plant is used as a vermifuge and to treat sore eyes, haematuria, rheumatism, dropsy, typhoid, asthma, blood disorders and is reportedly effective against leprosy. A decoction of the plant is used for the treatment of hysteria, dysentery and other stomach troubles. The roots are considered bitter, tonic, purgative, anthelmintic and diuretic. In ayurveda a paste made from the roots is considered specific ringworm and other skin ailments. The leaves are also used externally to relieve inflammatory swellings, rheumatism, wounds and sprains. The root bark is used as a quinine substitute to cure fever, and its decoction is used as a stimulant and as a specific medicine for gonorrhoea and hepatic troubles.
DETAILED DESCRIPTION OF THE INVENTION
Accordingly the present invention provides a herbal nutritious composition, comprising the extracts obtained from Tinospora cordifolia, 4-7 %, Glycyrrhyza glabra, 4-7 %, Adhatoda vasica 4-7%, Madhuca indica 8-15% and Cassia occidentalis 2-4 % and acceptable additives 60-80%.

In another embodiment, an herbal nutritious chocolate used in the fields of foods and
pharmaceuticals as a nutrient source, comprising the extracts obtained from Tinospora
cordifolia, Glycyrrhyza glabra, Adhatoda vasica, Madhuca indica and Cassia occidentalis with
acceptable additives.
In still another embodiment, herbal nutritious composition as, wherein the extracts/juice of the
plants are mixed in the ratio viz. Tinospora cordifolia (4-7 %), Glycyrrhyza glabra (4-7 %),
Adhatoda vasica (4-7 %), Madhuca indica (8-15 %), and Cassia occidentalis (2-4 %).
In another embodiment, herbal nutritious composition wherein said composition is in
conventional form of a semisolid/solid/granules or any other form.
In another embodiment, herbal nutritious composition wherein the extracts of plants may be
alcoholic or aqueous extract or mixture thereof.
In yet another embodiment, herbal nutritious composition wherein the alcohol used is ethanol.
In another embodiment, herbal nutritious composition wherein the said composition comprises
plant extract about 20-40% wt of the total formulation.
In still another embodiment, herbal nutritious composition wherein the additives selected are
starch, lactose, sugar, gum acacia and known lubricant or mixture thereof
In another embodiment, herbal nutritious composition wherein the plant extracts are obtained:
from plant parts selected from leaf, seeds, floral parts rhizome and aerial parts.
In yet another embodiment, herbal nutritious composition wherein the lubricants may be selected
from milk or its derivatives.
In another embodiment, the invention also provides a method for preparation of a herbal
nutritious composition, wherein the said method comprising:
a) Selecting parts of plant Tinospora cordifolia, Glycyrrhyza glabra, and Adhatoda vasica, to be extracted,
b) drying the plant material in shade,
c) powdering the dried plant material to a coarse powder,
d) sieving the coarse powder to prepare a decoction, adding an extracting agent and boiling to obtain concentrate,
e) adding floral parts of Madhuca indica and roasted seeds of Cassia occidentalis to the concentrate and spray drying one or more additives thereon to form a mixture and
6
f) heating the mixture obtained in step e) gently to form a paste.
In yet another embodiment, herbal nutritious composition wherein the formulation can be stored
at room temperature
In yet another embodiment, herbal nutritious composition wherein formulation has a shelf life of
2 years.
In another embodiment, herbal nutritious composition wherein the formulation showed as a
potent antioxidant activity.
In still another embodiment, herbal nutritious composition wherein the formulation showed as a
potent adaptogenic activity.
In another embodiment, herbal nutritious composition wherein the formulation showed as a
potent anti stress activity.
In yet another embodiment, herbal nutritious composition wherein the formulation showed as a
potent enzymatic super oxide dismutase enhancing activity.
In another embodiment, herbal nutritious composition wherein the formulation showed as a
potent lipid peroxidation inhibition.
In still another embodiment, herbal nutritious composition wherein the herbal nutritious
composition showed as a potent enzymatic enhancing catalase activity.
In another embodiment, herbal nutritious composition wherein the herbal nutritious composition
showed an enhanced protein content.
In yet another embodiment, herbal nutritious composition wherein the herbal nutritious
composition helpful in defending the body against the effects of stressful environment and
provide vitality and energy.
Following example are given by illustration
Formulatioinl (Fl):
1. Tinospora cordifolia (stem) - 7 %
2. Adhatoda vasica (Wl. PL) - 0%
3. Glycyrrhyza glabra (Rhizome) - 0 %
(Table Removed)
Formulatiom3 (F3):
1. Tinospora cordifolia (stem) - 0 %t
2. Adhatoda vasica (Wl ?l.) - 0%
3. Glycyrrhyza glabra (Rhizome) - 7 %
4. Madhuca indica (f[.parts) - \4%
5. Cassia occidentalis- 3 %
6. Spray dried milk powder - 10%)
7. Sugar- 10%
8. Gum acacia powder- 1 %
9. Lactose to make up 100 %>
Formulatioin4 (F4):
1. Tinospora cordifolia (stem) - 6 %
2. Adhatodavasica(Wl.PL.) -
3. Glycyrrhyza glabra (Rhizome)
4. Madhuca indica (fl.parts) -
5. Cassia occidentalis-
6. Spray dried milk powder -
7. Sugar-
8. Gum acacia powder-
9. Lactose
6% 6% 12% 3% 9% 9% 1% to make up 100 %
The above (1,2,3) dried material coarsely powdered with 16 x screen and water is added
which is heated on to concentrate. Out of which 300 ml of decoction is taken in it of
Madhuca indica (fl.parts) added along with powder of roasted seeds of Cassia
occidentalis followed by spray dried milk and gum acacia. Mixed properly through
mixer, and sieved through fine mesh no. 60, and consistency to paste is kept in molds &,
in oven to remove excess of moisture. Altering the first three ingredients, and taking
them altogether has provided four sets of combination which has been termed as
Table 1- Effect of formulation on chronic stress-induced changes in lipid peroxidaation, superoxide dismutase and catalase in rats.

(Table Removed)
Values are mean ± S.E.M.
P: ' NOTE:
No mortality was found in any of the treated group.
No gross abnormality in behavior was observed in the animal exposed with herbal preparation.
Formulation (Fl)- consisting of only extract of Tinospora cordifolia (stem) Formulation (F2) consisting of only extract Adhatoda vasica (Wl. PI.) Formulation (F3) consisting of only Glycyrrhyza glabra (Rhizome) where as Formulation (F4) consisting of all the above.
The above table 1 states that the herbal formulation F4 has potent antioxidant activity as evident by its ability to prevent lipid peroxidation from 0.71 to 0.28 and by the augmentation of the antioxidant enzymes viz. superoxide dismutase (256.8 in vehicle under stress to 162.3 at F4) and catalse (19.5 in vehicle under stress to 31.3 at F4) when compared to the vehicle which is plain water. Thus the herbal formulation prepared exhibits potent antioxidant activity under stress conditions and hence is a good rejuvenator.
Lipid peroxidation: The fundic part liver was homogenized (5%) in ice-cold 0.9% NaCl in glass homogenizer for 30 s. The homogenate was centrifuged for 10 min A volume of the homogenate (0.20 ml) was transferred to a vial and was mixed with 0.2 ml of a 8.1% (w/v) sodium dodecyl sulfate solution, 1.50 ml of a 20% acetic acid solution (adjusted to pH 3.5 with NaOH) and 1.50 ml of a 0.8% (w/v) solution of thiobarbituric acid (TBA) and the final volume was adjusted to 4.0 ml with distilled water. Each vial was tightly capped and heated in a boiling water bath for 60 min. The vials were then cooled under running water. Equal volumes of tissue blank or test samples and 10% trichloroacetic acid were transferred into a centrifuge tube and centrifuged. The absorbance of the supernatant fraction was measured at 532 nm. Control experiment was processed using the same experimental procedure except the TBA solution was replaced with distilled water. 1,1,3,3-Tetraethoxypropan was used as standard for calibration of the curve and is expressed as nanomoles per milligram protein. SOD and Catalase:
Decomposition of H2O2 in presence of catalase (CAT) was followed at 240 nm. One unit (U) of catalase was defined as the amount of enzyme required to decompose 1 µmol of H2O2 per minute, at 25 °C and pH 7.0. Results are expressed as units of CAT activity per milligram of protein. Superoxide dismutase (SOD) activity was estimated by the
inhibition of nicotinamide adenine dinucleotide (reduced)-phenazine methosulphate-nitrobluetetrazolium reaction system. One unit of the enzyme is equivalent to 50% inhibition in the formazan formation in 1 min at room temperature (25 °C) and the resuhs have been expressed as units of SOD activity per milligram of protein.
Table 2: Effect of formulation on hypoxia and swimming performance time in mice.

(Table Removed)
Values are mean ± S.E.M.
P: '^ NOTE:
No mortality was found in any of the treated group.
No gross abnormality in behavior was observed in the animal exposed with herbal
preparation.
Formulation (Fl)- consisting of only extract of Tinospora cordifolia (stem)
Formulation (F2) consisting of only extract Adhatoda vasica (Wl. PI.) Formulation
(F3) consisting of only Glycyrrhyza glabra (Rhizome) where as Formulation (F4)
consisting of all the above.
As evident from table 2 the herbal formulation F4 exhibits potent adaptogenic activity as
evident from increase in the hypoxia time from 28.11 under stress to 45.89 upon
administration of F4 and increase in swimming endurance from 295.12 to 423.21 upon
administration of F4.
Commercially available sample did not significant activity in hypoxia time and swimming endurance tests of the adaptogenic activity. Procedures for adaptogenic activity Hypoxia time:
The animals were placed in an empty glass jar of 300 mL capacity attached with an
electronic watch; the jars were made air tight with greased glass stoppers and the time
until the onset of convulsion was recorded (Singh B et al, 2001, Phytotherapy Research,
15; 311-318).
Swimming performance time:
The animals were allowed to swim inside a perpex glass beaker (30 cm high with 20 cm
diameter containing water up to 25cm high) maintained at 26° ± 1° C with a continuous
air current from the bottom. The end point of swimming endurance was taken as when
the mice remained at the bottom for more than 10s (Singh B et al, 2001, Phytotherapy
Research, 15; 311-318).




We claim:
1. A herbal nutritious composition, comprising the extracts obtained from Tinospora cordifolia, 4-7 %, Glycyrrhyza glabra, 4-7 %, Adhatoda vasica 4-7%, Madhuca indica 8-15% and Cassia occidentalis 2-4 % and acceptable additives 60-80%.
2. The herbal nutritious composition as claimed in claim 1, wherein said composition is in the form of a semisolid/solid/granules or any other form.
3. The herbal nutritious composition as claimed in claim 1, wherein the extracts in the form ofjuices.
4. The herbal nutritious composition as claimed in claim 1, wherein the extracts of plants is alcoholic extract, aqueous extract or mixture thereof.
5. The herbal nutritious composition as claimed in claim 4, wherein the alcohol used is ethanol.
6. The herbal nutritious composition as claimed in claim 1, wherein the extract comprises 20-40% wt of the composition.
7. The herbal nutritious composition as claimed in claim 1, wherein the additives are selected from starch, lactose, sugar, gum acacia and lubricants or mixture thereof.
8. The herbal nutritious composition as claimed in claim 1, wherein the plant extracts are obtained from plant parts selected from leaf, seeds, floral parts rhizome and aerial parts.
9. The herbal nutritious composition as claimed in claim 1, wherein the lubricants used is selected from milk or its derivatives.
10. A method for preparation of a herbal nutritious composition as claimed in claim 1, wherein the said method comprising:
a) Selecting parts of plant Tinospora cordifolia, Glycyrrhyza glabra, and Adhatoda vasica, to be extracted.
b) drying the plant material in shade,
c) powdering the dried plant material to a coarse powder,
d) sieving the coarse powder to prepare a decoction, adding an extracting agent and boiling to obtain concentrate,
e) adding floral parts of Madhuca indica and roasted seeds of Cassia occidentalis to the concentrate and spray drying one or more additives thereon to form a mixture and
f) heating the mixture obtained in step e) gently to form a paste.
11. A method as claimed in claim 10, wherein the paste is transferred to a mold and cooled to obtain a solid or semi-solid composition.
12. A method as claimed in claim 10, wherein the paste is used to form granules.
13. A method as claimed in claim 11, wherein the molding is carried out by cooling the paste in molds at a temperature in the range of -4 to -20 degree C.
14. A herbal nutritious composition and method for preparation thereof substantially as herein described with reference to the examples.

Documents:

2507-DEL-2004-1-Correspondence-Others-(21-02-2011).pdf

2507-DEL-2004-1-Petition 137-(21-02-2011).pdf

2507-DEL-2004-Abstract-(21-02-2011).pdf

2507-del-2004-abstract.pdf

2507-DEL-2004-Claims-(21-02-2011).pdf

2507-del-2004-claims.pdf

2507-DEL-2004-Correspondence-Others-(21-02-2011).pdf

2507-del-2004-correspondence-others.pdf

2507-DEL-2004-Description (Complete)-(21-02-2011).pdf

2507-del-2004-description (complete).pdf

2507-DEL-2004-Form-1-(21-02-2011).pdf

2507-del-2004-form-1.pdf

2507-del-2004-form-18.pdf

2507-del-2004-form-2.pdf

2507-DEL-2004-Form-3-(21-02-2011).pdf

2507-del-2004-form-3.pdf

2507-del-2004-form-5.pdf


Patent Number 248784
Indian Patent Application Number 2507/DEL/2004
PG Journal Number 34/2011
Publication Date 26-Aug-2011
Grant Date 24-Aug-2011
Date of Filing 16-Dec-2004
Name of Patentee COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH
Applicant Address RAFI MARG, NEW DELHI-110 001, INDIA
Inventors:
# Inventor's Name Inventor's Address
1 PALPU PUSHPANGADAN N.B.R.I LUCKNOW INDIA
2 AJAY KUMAR SINGH RAWAT N.B.R.I LUCKNOW INDIA
3 CHANDANA VENKATESWARA RAO N.B.R.I LUCKNOW INDIA
4 SANJEEV KUMAR OJHA N.B.R.I LUCKNOW INDIA
5 GADDAM DAYANAND REDDY N.B.R.I LUCKNOW INDIA
PCT International Classification Number A61K
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA