Title of Invention

FORMULATION OF A SYNERGISTIC INSECTICIDAL COMPOSITION AS A CYCLODEXTRIN-COMPLEX

Abstract Abstract The present invention provides a new insecticide formulation based on cyclodextrin characterised in that the active substance (insecticide and/or insect growth regulator), and a compound synergistic with the active substance, are complexed simultaneously with cyclodextrin. The formulation presents as a solid or as a solid/oil composition, and is soluble or completely emulsifiable in water or in aqueous mixtures of water miscible solvents. The activity of the present formulations was found to be greater than that of a mixture of the two active components each complexed separately with cyclodextrin, for the same dose. The preparation process of said formulation and its use as an insecticide in agriculture, for veterinary use or to eliminate household insects, are further aspects of the present invention.
Full Text

INSECTICIDE FORMULATION FIELD OF THE INVENTION
The present invention relates to the field of insecticide compositions, in particular those in which the insecticidal agent is mixed with substances inhibitory to the detoxification mechanisms of insects. New compositions are described in which the effect of insecticide and synergistic substance is further enhanced by simultaneous complexing with cyclodextrin. PRIOR ART
The problem of tolerance and resistance to pesticide activity is particularly serious and of growing importance, leading to the ever more difficult control and eradication of damaging insects in agriculture, veterinary and domestic hygiene applications. Many insects have strengthened their natural defences and their immune and enzymatic systems against toxins with which they come into contact such that, in order to destroy them, increasing doses or the continuous use of new insecticides or insect growth inhibitors are necessary, with the resultant greater risk and damage to the whole ecosystem and food chain through to man, and consequent rising costs.
The use of substances such as piperonyl butoxide (PBO) and its analogues which are able, in synergistic combination with insecticides, to inhibit the activity of certain insect metabolic enzymes involved in detoxification and pesticide resistance processes, thus able to enhance in vitro efficacy, is known in the literature [see for example Gunning et al., "Piperonyl Butoxide", p.215-225, Academic Press (1998)], their use in vivo having also been suggested. In order to better demonstrate synergistic activities, particularly in cases where the insect is most resistant, treatment with the synergistic product at varying times and prior to the insecticide or a repeated insecticide treatment was proposed; pre-treatment with the synergistic compound is particularly beneficial in that subsequent exposure to the insecticide takes place on an already sensitised insect, being therefore more effective. Separate administrations however are not very practical and are economically unfavourable when compared with a single application of the two components. Also described in the literature and in patents are formulations of insecticides and

insect"growth regulators in cyclodextnns (CD) |see tor example L. szerne ex ai,• "Cyclodextrins in Pesticides" in "Comprehensive Supramolecular Chemistry",
p.503514, Elsevier (1996), US 3,846,551]- There are many principle reasons for
using such inclusion complexes: modification of the physicochemical properties of
pesticides, greater stability, the increased wettability and bioavailability of poorly
soluble and poorly absorbable pesticides etc.
a, β and y cyclodextrins are natural or semi-synthetic cyclic oligosaccharides,
generally non-toxic and biodegradable; β-CD and some of its derivatives, such as
the hydroxypropyl (HP-β-CD) and the sulfobutyl ether (SBE-β-CD) are particularly
preferred for applications.
US 3,846,551 states that the activity of insecticides complexed with CD is better
than that of uncomplexed insecticides. However, formulations also containing
simultaneously a synergistic compound have never been described. PBO has
also been prepared as a complex with CD (US 4,524,068), shown to be more
effective as an insecticide synergist than uncomplexed PBO; again in this case,
however, the tests were carried out on mixtures of uncomplexed insecticide and
PBO/CD and not on a single formulation.
The present invention proposes to overcome the drawbacks of the known art and
to improve significantly the performances of commercially known insecticides or
insect growth regulators by means of a single treatment; a further aspect of the
invention is to obtain an economical, industrial scale preparation process for said
formulation, with low or no toxicity to users.
SUMMARY
The present invention relates to a new formulation characterised by the
simultaneous presence, as a complex with cyclodextrin, of
(i) an active principle being a component with insecticidal activity and/or a
component with insect growth regulator activity and
(ii) a component able to synergistically enhance the activity of the active principle.
The invention also refers to the preparation of said formulation and its use in
agriculture, in veterinary practise and for eliminating household insects. The
formulation is obtained by subjecting both the insecticide and the synergistic
compound jointly to complexing with CD.

The aforesaid formulation is also effective in cases where an insect is tolerant and resistant to insecticidai activity or to growth regulation by the same active substance, giving rise, as an insecticide and for the same dose, to an insect mortality substantially greater than that demonstrated by the same active components used in mixtures as such or complexed separately with cyclodextrin. DETAILED DESCRIPTION OF THE INVENTION
Any cyclodextrin can be used for the purposes of the present invention. For example the cyclodextrin can be a, (3, or y cyclodextrin as such or, if appropriate, derivatised to increase the hydrophilic or hydrophobic nature thereof. Particularly preferred are β-CD, y-CD and HP-β-CD, (β-CD being more preferred due to its lower cost. The insecticides which can be used in the present invention preferably contain within their structure at least one aromatic carbocycle or heterocycle. Particularly preferred are those of pyrethroid structure such as Allethrin, Bioallethrin, Tetramethrin, Prallethrin, Cypermethrins (a-Cypermethrin, J3-Cypermethrin, (_)-Cypermethrin), Esbiothrin, Perrnethrin, Fenpropathrin, Transfluthrin, Bifenthrin, Resmethrin, Bioresmethrin, Fenvalerate, Esfenvalerate, Tetramethrin, Imiprothrin, Phenothrin, β-Cyfluthrin, Deltamethrin, Cyhalothrin, Etofenprox, Silaflluofen etc., and their enantiomeric and/or diastereoisomeric mixtures. Cypermethrin, Fenvalerate, Deltamethrin and β-Cyfluthrin and their enantiomeric and/or diastereoisomeric mixtures are most preferred. The quantity of insecticide relative to cyclodextrin is preferably between 5% and 40% (weight/weight) and even more preferably between 10% and 25%. Suitable insect growth regulators include, for example, Brevioxime, Buprofezin Ketoconazole, Teflubenzuron .
The quantity of growth regulator relative to cyclodextrin is preferably between 0.01% and 5% (weight/weight) and even more preferably between 0.5% and 3%. The components able to synergistically enhance the activity of the active principle (here referred to in brief as "synergistic compounds") are per se known and already in use. Such products are inhibitors of insect detoxifying enzymes, for example esterases and oxidases. Preferred examples of synergistic compounds are piperonylbutoxide and sesamol. Piperonylbutoxide is particularly preferred. The synergistic compounds can be used as such or already preformulated with

additives; one example of a commercially available preformulation is known as
PB80EC-NF; it contains 88% PBO and 12% emulsifier (dodecylbenzenesulfonate,
also known as SOITEM).
The quantity of synergistic compound relative to cyclodextrin is preferably between
10% and 100% (weight/weight) and even more preferably between 25% and 95%;
these percentages refer to the quantity of pure synergistic compound, hence
excluding additives that may be present in the preformulation.
The quantity of insecticide relative to the synergistic compound is preferably
between 5% and 50% (weight/weight) and even more preferably between 10%
and 30%.
Emuisifiers, UV stabilisers, antioxidants and other additives which are not
particular to insecticidal activity but are useful for the specific application, can also
be present in the aforesaid formulation.
The quantity of said additives relative to cyclodextrin is preferably between 0 and
30% (weight/weight) and even more preferably between 5% and 15%. These
percentages refer to all additives present, including those already present in the
preformulations of the active principles used.
Emuisifiers which can be used are, for example, the aforementioned
dodecyibenzene-suifate, llynOsuifonaies, phosphoiipids and polyethylene yiyCois.
UV stabilisers which can be used are for example 2-hydroxy-4-
methoxybenzophenone, 2-hydroxy-4-octoxy-benzophenone and 4-hydroxy-
2,2,6,6- tetramethylpiperidine sebacate.
Antioxidants which can be used are for example 2,6-ditertbutyM-hydroxy-toluene-
The composition of the invention is formulated preferably as a solid or as a solid/oil
composition; said formulations can be used as such, or previously
dissolved/emulsified in water or in aqueous solutions of water miscible solvents,
such as a C1-4 alcohol; said aqueous solutions contain from 1% to 99% by weight,
preferably from 5% to 60% by weight, of water miscible solvent.
The preparation procedure for the aforedescribed formulations is characterised by
a simultaneous complexing in cyclodextrin of the synergistic compound and the
insecticide and/or growth regulator.
More specifically, the procedure comprises the following passages:

(a) preparihg a solution or suspension of the synergistic compound and the
insecticide'fend/or growth regulator in a suitable solvent; the solvent is preferably
an alcohol, for example ethanol or 2-propanoI.
(b) preparing a solution of cyclodextrin in water or in mixtures of water/ water
miscible organic solvent; dissolution of the CD can be conveniently facilitated by
heating (for example by maintaining it at 70°C-90°C for 30-90 minutes).
(c) adding the solution/suspension obtained in (a) to the solution obtained in (b);
preferably the solution/suspension in (a) is added slowly, e.g. over 2-10 hours
(more preferably 4-8 hours), at a temperature of 20°C-90°C, (more preferably 30°C-70°C).
Following addition of the active components, the complexing reaction goes to completion within a period generally between 12 and 36 hours (preferably 18-24 hours) maintaining the mixture under agitation, at a temperature between 20°C and 90°C (preferably 30°C-70°C).
The final CD complex, which includes both the synergistic compound and the insecticide and/or growth regulator, is recovered from the reaction mixture by known methods, such as filtration, drying or lyophilisation.
A further aspect of the invention is the use of the aforesaid formulations as an insecticide in agriculture, for veterinary use or to eliminate household insects. The joint complexing of insecticide and/or growth regulator and synergistic compound with cyclodextrin has surprisingly resulted in a significant increase in the effectiveness of the composition compared with a mixture of the two components complexed individually. By means of the invention an enhancement of the interaction between insecticide and synergistic compound is achieved; in comparative trials carried out by the inventors this enhancement has always been over 50%: the effect is therefore of substantial proportions. Enhancement of activity gives rise to several industrially significant advantages: for instance by using the same amounts of active substance, synergistic m compositions of greater activity can be obtained; or insecticide compositions with an effectiveness equal to known compositions can be obtained but with the use of less active substances; the use of less active substances results in a lower product cost, reduced environmental impact from the production process, as well

as the final composition having a lower volume/weight, of further practical
advantage to the crop spraying operator.
Therefore highly effective insecticide formulations, of lower cost than known
formulations, were unexpectedly obtained with the present invention.
The following non-limiting examples are therefore useful to illustrate the invention.
EXPERIMENTAL PART
Example 1
Procedure for preparing the formulation and stability measurements.
βCD (2g) in distilled water (20 ml) is introduced at 80°C into a 2-neck flask,
equipped with cooler and nitrogen outlet The solution is left for 1 hour at 80°C
under agitation. After this period, a 96% ethanol solution (25 ml) containing the
synergistic compound and the insecticide and/or growth regulator in the required
proportions, is added in portions over a 6 hour period at 65°C. The mixture is left
at 70°C under agitation for a further 21 hours, then the mixture is left to cool at
room temperature under agitation and finally allowed to decant for 4 hours. The
solid is filtered off and the solution dried under vacuum.
By means of the aforesaid procedure the following products were prepared:
- inclusion complex pCD-fenvalerate and PBO (*) (a white water soluble solid)
- inclusion complexes pCD-cypermeLhrinS and PBO (*) (a white water soiubie
solid).
(*): the PBO was used starting from a commercial composition known as PB80EC-
NF, containing 88% PBO and 12% SOITEM.
The inclusion complexes were stable in the solid phase for at least 30 days at
23°C.
Example 2
Preparation of a formulation based on fenvaierate
Operating as in example 1, a formulation was prepared starting from 1.9 g βCD,
0.35 g fenvaierate and 1.6 g PB80EC-NF.
Example 3
Preparation of a formulation based on cypermethrin
Operating as in example 1, a formulation was prepared starting from 1.9 g |3CD,
0.35 g acypermethrin and 1.6 g PB80EC-NF.

Example 4
Mortality assay
In a mortality assay conducted as reported in the literature [Gunning R.V. et al., J. Econ. Entomol. 77, 1283-1287 (1984)] a cotton aphid specimen was used showing a resistance to cypermethrin at least 7000 times greater than the lethal dose (LD50). Using the formulation prepared as in example 3 at an active principle concentration of less than the LD50, complete mortality of the insect was obtained, while with traditional formulations containing the same doses of cypermethrin or with mixtures of cypermethrin and PBO encapsulated separately and at equal doses, the mortality was always Preparation of a formulation based on cypermethrin
pCD (50 g) in distilled water (630 ml) at 75°C was introduced into a two-neck flask provided with cooler and nitrogen outlet. The solution was maintained under stirring at 75 °C for 1 hour. Thereafter a solution of 2-propanol (790 ml) containing PBO/SOITEM (98/2, 13.4 g) and acypermethrin (5.4 g) was added in portions at the temperature of 70-75°C during 6 hours. The mixture was maintained under stirring at 75 °C for another 18 hours, then the mixture was allowed to cool at room temperature under stirring during 90 minutes and finally kept still for 3 hours. The solution was dried under vacuum obtaining, as dry residue, a formulation based on cypermethrin.
In such dry residue, containing 8.6% water and 2% 2-propanol, the PBO/cypermethrin ratio was comprised between 2.7 and 3 [as from GC-FID and 1H NMR (DMSO-d6) analysis, respectively] whereas the amount (weight/weight) of PCD was about 72% [1H NMR (DMSO-d6)] Example 6
Preparation of a formulation based on bifenthrin
PCD (2g) in distilled water (20 ml) at 75°C was introduced into a two-neck flask
» provided with cooler and nitrogen outlet The solution was maintained under
stirring at 75 °C for 1 hour. Thereafter, a solution of 2-propanol (25 ml) containing
PBO/SOITEM (98/2, 0,52 g) and bifenthrin (0.22 g) was added in portions at the

temperature of 75°C during 6 hours. The mixture was maintained under stirring at
75 °C for another 18 hours, then the mixture was cooled at room temperature
under Stirling during 2 hours and finally kept still for 3 hours. The solution was
dried under vacuum, obtaining, as dry residue, a formulation based on bifenthrin.
Example 7 _„
Preparation of a formulation based on cyfluthrin
The formulation was prepared according to example 6, starting from 2 g PCD,
0.53g PBO/SOITEM (98/2) and 0.22g pcyfluthrin.
Example 8
Preparation of a formulation based on cyhalothrin
The formulation was prepared according to example 6, starting from 5 g PCD,
1.33g PBO/SOITEM (98/2) and 0.58g Acyhalothrin.
Example 9
Preparation of a formulation based on deltamethrin
The formulation was prepared according to example 6, starting from 5 g pCD,
1.33g PBO/SOITEM (98/2) and 0.65g deltamethrin.
Example 10
Preparation of a formulation based on fenvalerate
The formulation was prepared according to example 6, starting from 5 g βCD ,
..._ -_ . - _ ,_ _,,_.__ —w __ j— _, .^ -. — _ ^ j ,
1.33g PBO/SOITEM (98/2) and 0.54g fenvalerate.
In the dry residue thus obtained the PBO/fenvalerate ratio was about 4.2 [as from
1H NMR (DMSO-e/6) analysis], whereas the 3CD content (weight/weight) was
about 76% [1H NMR (DMSO-d6)analysis].
Example 11
Preparation of a formulation based on cypermethrin
pCD (2g) in distilled water (20 ml) at 75°C was introduced into a two-neck flask
provided with cooler and nitrogen outlet The solution was maintained under
stirring at 75 °C for 1 hour. Thereafter, a solution of 2-propanol (25 ml) containing
PBO/SOITEM (96/4, 0.93 g) and acypermethrin (0.36 g) was added in portions
during 6 hours at the temperature of 70-75 °C. The mixture was maintained under
stirring at 75 °C for another 18 hours, then the mixture was cooled at room
temperature under stirring during 2 hours and finally kept still for 3 hours. The oil

phase was thus removed and the overlaying solution was dried under vacuum
obtaining", 'as dry residue, a formulation based on cypermethrin.
Example 12
Preparation of a formulation based on cypermethrin
(3CD (1 g) in distilled water (20 ml) at 75°C was introduced into a two-neck flask
provided with cooler and nitrogen outlet. The solution was maintained under
stirring at 75 °C for 1 hour. Thereafter the solution is cooled at 50°C, then at this
temperature a solution of 2-propanol (25 ml) containing PBO/SOITEM (98/2, 0.26
g) and acypermethrin (0.11 g) was added in portions during 6 hours. The mixture
was maintained under stirring at 50°C for another 18 hours, then the mixture was
allowed to cool at room temperature under stirring during 2 hours and finally kept
still for 3 hours. The solid (4%) is filtered off and the solution containing the
inclusion complex is dried under vacuum obtaining, as dry residue, a formulation
based on cypermethrin.
In such dry residue the PBO/cypermethrin ratio was about 4/1 [as from 1H NMR
(DMSO-c/6) analysis] whereas the amount (weight/weight) of pCD was about
73% [1H NMR (DMSO-d/6) analysis].
Example 13
Preparation of a formulation based on ketoconazole
The formulation was prepared according to example 6, starting from 2 g pCD,
0.53g PBO/SOITEM (98/2) and 0.06g ketoconazole.
Example 14
Preparation of a formulation based on cypermethrin
βCD (1g) in distilled water (20 ml) at 75°C was introduced into a two-neck flask
provided with cooler and nitrogen outlet The solution was maintained under
stirring at 75 °C for 1 hour. Thereafter, the solution was cooled at 50°C; then, at
this temperature, a solution of 2-propanol (50 ml) containing PBO/SOITEM (98/2,
0.26 g) and acypermethrin (0.11 g) was added in portions during 6 hours. The
mixture was maintained under stirring at 50°C for another 90 minutes, then the
mixture was cooled at room temperature under stirring during 90 minutes and
finally kept still for 1 hour. The separated solution was dried under vacuum
obtaining, as dry residue, a formulation based on cypermethrin.

Example 15
Preparation of a formulation based on pyrethrum extracts βCD (2g) in distilled water (20 ml) at 75°C was introduced into a two-neck flask provided with cooler and nitrogen outlet The solution was maintained under stirring at 75 °C for 1 hour. Thereafter, a solution of 2-propanoI (25 ml) containing PBO/SOITEM (98/2, 0.53 g) and the pyrethrum extracts at 25% w/w (0.70 g) was added in portions during 6 hours at the temperature of 70-75 °C. The mixture was maintained under stirring at 75 °C for another 18 hours, then the mixture was cooled at room temperature under stirring during 2 hours and finally kept still for 3 hours. The solid was filtered off, and the overlaying solution was dried under vacuum obtaining, as dry residue, a formulation based on pyrethrum extracts-Example 16
Preparation of a formulation based on cyperrnethrin
3CD (5 g) in distilled water (50 ml) at 75°C was introduced into a two-neck flask provided with cooler and nitrogen outlet The solution was maintained under stirring at 80°C for 1 hour. Thereafter a solution of 2-propanol (63 ml) containing PBO/SOITEM (98/2, 2.24 g) and acypermethrin (0.91 g) was added in portions at the temperature of 75°C during 6 hours. The mixture was maintained under stirring at 75 °C for another 18 hours, then the mixture was allowed to coo! at room temperature under stirring during 2 hours and finally kept still for 3 hours. The oil phase was removed and the overlaying solution was dried under vacuum obtaining, as dry residue, a formulation based on cypermethrin. In such dry residue the PBO/cypermethrin ratio was about 11 [as from 1H NMR (DMSO-d/6) analysis] whereas the amount (weight/weight) of pCD was about 75% [1H NMR (DMSO-d6)]. Example 17
Preparation of a formulation based on cypermethrin
PCD (50 g) in distilled water (500 ml) at 75°C was introduced Into a two-neck flask provided with cooler and nitrogen outlet- The solution was maintained under stirring at 75°C for 1 hour. Thereafter a solution of 96% ethanol (625 ml) containing PB80EG-NF (42.7 g) and acypermethrin (9.15 g) was added in portions at the temperature of 70-75°C during 6 hours. The mixture was maintained under

stirring at 70°C for another 18 hours, then the mixture was allowed to cool at room
temperature under stirring during 2 hours and finally kept still for 3 hours. The oil
phase was removed and the overlaying solution was dried under vacuum
obtaining, as dry residue, a formulation based on cypermethrin.
In such dry residue the PBO/cypermethrin ratio was about 2.8 [as from 1H NMR
(DMSO-d6) analysis] whereas the amount (weight/weight) of βCD was about
60% [1H NMR (DMSO-d6)].
Example 18
Mortality assay
A mortality assay was performed as reported in example 4 on a Bemisia Tabaci
strain
(B-Bioiype) ( Silver leaf whitefly) using the formulation described in example 16,
dissolved in 10 ml Agral 90. The results are shown in table 1. Data obtained with
acypermethrin dissolved in 10 ml Agral 90, and with placebo (10 ml Agral 90) are
shown as a reference. In all tests, concentration values are referred to the amount
of active principle.

Example 19
Mortality assay
A mortality assay was performed as reported in example 4 on a cotton aphid strain (Aphis Gossypii) using the formulation described in example 10, dissolved in 10 ml Agral 90. The results are shown in table 2. Data obtained with fenvalerate dissolved in 10 ml Agral 90, and with placebo (10 ml Agral 90) are shown as a

referencei In all tests, concentration values are reterred to tne amount or active principle

Example 20
Mortality assay
A mortality assay was performed as reported in example 4 on a Heicoverpa Armigera strain ( Cotton Bollworm) using the formulation described in example 17, dissolved in 10 ml Agral 90. The results are shown in table 3. Data obtained with acypermethfin dissolved in 10 ml Ayral90, and with a mixture of ucypermethrin /PBO dissolved in 10 ml of Agral 90 (the PBO content being 0.2% with respect to acypermethrin) are shown as a reference. In all tests, concentration values are referred to the amount of active principle-


NEW SET OF CLAIMS
1. Insecticidal composition comprising:
(0 an active principle being a component with insecticidal activity belonging to the
pyretnroid class and/or a component with insect growth regulator activity
(ii) a component able to synergistically enhance the activity of the active principle,
said composition being obtainable by subjecting both components (i) and (ii) to
simutaneous complexing with cyclodextrin.
2. Composition as claimed in claim 1, wherein the cyclodextrin is chosen from a, p,
y cyclodextrin, HP-B-cyclodextrin and SBE-B-cyclodextrin.
3. Composition as claimed in claims 1-2, wherein the quantity of insecticide
relative to cyclodextrin is between 5% and 40% (weight/weight).
4. Composition as claimed in claims 1-3, wherein the quantity of growth regulator
relative to cyclodextrin is between 0.01% and 5% (weight/weight).

5. Composition as claimed in claims 1-3, wherein the compound able to
synergistically enhance the activity of the active principle is chosen from piperonyl
butoxide and sesamol.
6. Composition as claimed in claims 1-4, wherein the quantity of synergistic
compound relative to cyclodextrin is between 10 and 100% (weight/ weight).
7. Composition as claimed in claims 1-5, wherein the quantity of insecticide
relative to the synergistic compound is between 5% and 50% (weight/weight).
8. Composition as claimed in claims 1-6, comprising emulsifiers, UV stabilisers,
antioxidants and other additives in a quantity between 0 and 30% (weight/weight).
9. Composition as claimed in claims 1-7, formulated for use in solid form or as a
solid/oil composition, possibly dissolved/emulsified in water or in aqueous
solutions of water miscible solvents.

10. Process for preparing compositions described in claims 1-9, characterised by
simultaneously complexing the synergistic compound and the insecticide and/or
growth regulator in cyclodextrin.
11. Process as claimed in claim 10, comprising the following passages:
(a) preparing a solution or suspension of the synergistic compound and the insecticide and/or growth regulator in a suitable solvent;

miscible organic solvents;
(c) adding the solution/suspension obtained in (a) to the solution obtained in (b).
13. Use of a composition as claimed in claims 1-9, as an insecticide in .agriculture,
or for eliminating household insects. "~~
13. Use of a composition as claimed in claims 1-9, in preparing a veterinary
formulation of use as an insecticide.


Documents:

1872-CHENP-2006 AMENDED PAGES OF SPECIFICATION 19-04-2011.pdf

1872-CHENP-2006 AMENDED CLAIMS 19-04-2011.pdf

1872-CHENP-2006 CORRESPONDENCE OTHERS 14-07-2010.pdf

1872-CHENP-2006 CORRESPONDENCE OTHERS.pdf

1872-CHENP-2006 CORRESPONDENCE PO.pdf

1872-CHENP-2006 EXAMINATION REPORT REPLY RECIEVED 19-04-2011.pdf

1872-CHENP-2006 FORM-18.pdf

1872-chenp-2006 form-3 19-04-2011.pdf

1872-CHENP-2006 POWER OF ATTORNEY 19-04-2011.pdf

1872-CHENP-2006 CORRESPONDENCE OTHERS 15-09-2009.pdf

1872-chenp-2006-abstract.pdf

1872-chenp-2006-claims.pdf

1872-chenp-2006-correspondnece-others.pdf

1872-chenp-2006-description(complete).pdf

1872-chenp-2006-form 1.pdf

1872-chenp-2006-form 3.pdf

1872-chenp-2006-form 5.pdf

1872-chenp-2006-pct.pdf


Patent Number 248072
Indian Patent Application Number 1872/CHENP/2006
PG Journal Number 24/2011
Publication Date 17-Jun-2011
Grant Date 14-Jun-2011
Date of Filing 26-May-2006
Name of Patentee ENDURA S.P.A.
Applicant Address Viale Pietramellara, 5, I-40125 BOLOGNA
Inventors:
# Inventor's Name Inventor's Address
1 PICCOLO, Oreste Via Borno, 5, I-23896 SIRTORI
2 DELOGU, Giovanna Via Ugo La Malfa, 26, I-07100 SASSARI
3 BORZATTA, Valerio Via Bellettini, 20, I-40127 BOLOGNA
PCT International Classification Number A01N25/10; A01N37/38; A01N43/30
PCT International Application Number PCT/EP2004/052665
PCT International Filing date 2004-10-26
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 MI2003A002088 2003-10-27 Italy