Title of Invention

"A PROCESS OF PREPARATION OF BACTERIAL SUSPENSION USEFUL FOR BIOLOGICAL CONTROL OF TASAR SILKWORM DISEASES"

Abstract This invention relates to a Process of preparation of bacterial suspension which is useful for Biological Control of Tasar Silkworm Diseases comprising the steps of, (a) isolation and purification of Brevibacillus laterosporus R-39 from Terminalia arjuna leaf, which is potent antagonistic bacteria against the pathogenic microorganisms of tasar silkworm Antheraea mylitta D; (b) mass multiplication of purified leaf bacteria characterized in that the important parameters comprising of: Inoculum dose (104 cell/ml); growth temperature (35+2°C); pH of 6.8 to 7.2 for bacterial growth; Time 36-42 hrs for cell multiplication and harvesting; Bacterial growth medium consisting of Tryptone 10gm; yeast extract 5gm; sodium chloride 5g/litre of dislitted water, at pH 7.0; (c) harvesting of live bacterial mass; (d) its storage in ampules with the critical concentration of bacterial suspension 108-109 cells/ml; (e) concentration of bacteria for foliar spray, wherein critical concentration is of 105-106 cells/ml, using soil water suspension as carrier.
Full Text FIELD OF INVENTION
This invention is related to a process for biological control of Tasar silkworm diseases. This process is used for enhancing the silk cocoon production.
PRIOR ART
Tasar silk worm (Antheraea mylitta D) suffers from various diseases, which results in the loss of crop estimated to the tune of 30-40%. For the prevention and control of silkworm diseases, it is known to provide various chemicals/drugs e.g. Antiprotozoan drugs, chemical formulation (Tasar Keet Oushod, Sodium Hypochlorite, Asiphor), antibiotics (Septran, Chloramphenicol) and disinfectants (Formalin, Bleaching Powder). However, the application of same is difficult for the farmers to adopt and not fully effective.
An Indian Patent (No: 192151) known in the art relates to a process for the preparation of novel composition useful as feed supplement for silkworm for enhancing the silk production comprising mixing of compound (i) stigmasterol in the range of 1 to 8 part (ii) 20-hydroxyecdysone compound in the range of 0.25 to 8 part and (iii) silenside in the range of 0.25 to 8 part. Thus, this Patent uses all chemicals.
Application of chemicals/drugs is very much detrimental to the silkworm and its host plants leading to harmful consequences of the flora and fauna. The chemicals/drugs used against the silkworm diseases are expensive and not very specific against pathogens responsible for the viral and bacterial diseases of silkworm. Moreover, chemicals/drugs are not eco-friendly and may cause damage to the surrounding agricultural crops and the environment. The tasar farmers who are poor and illiterate can not normally follow the accuracy of the method in preparation of the chemicals/drugs before application which may cause application of higher doses over the actual recommended dose. This in turn may spoil the whole crops since tiny tasar silkworm can not sustain the ill effect of chemicals.
OBJECTS OF THE INVENTION
An object of the present invention is to propose a process for biological control of Tasar silkworm diseases which does not cause environmental pollution.
Another object of the present invention is to propose a process for biological control of Tasar silkworm diseases which is easy in application and ecofriendly.
Further object of the present invention is to propose a process for biological control of Tasar silkworm diseases which protects the tasar silk crop a»d improves the cocoon production.
Still further object of the present invention is to propose a process for biological control of Tasar silkworm diseases which minimizes the crop loss by 20-25% caused due to various diseases of tasar silkworm.
Still another object of the present invention is to propose a process for biological control of Tasar silkworm diseases which improves the tasar cacoon productivity to the tune of 12-15 cocoon per laying (number of eggs laid by a tasar silkmoth).
Yet further object of the present invention is to propose a process for biological control of Tasar silkworm diseases which is cheaper as compared to the existing art.
STATEMENT OF INVENTION
According to this invention it provides a process for biological control of Tasar silkworm diseases comprising steps of: isolation of bacteria from the leaves of Arjuna plant, purification and multiplication of bacteria characterized by the steps of standarization of the purified bacteria followed by preparation of bacterial suspension by mixing soil water with bacteria for foliar application during silkworm rearing.
DETAIL DESCRIPTION OF THE PRESENT INVENTION
The process for biological control of Tasar silkworm diseases uses antagonistic bacteria available on the leaf Surface Microflora (LSM) of Tasar food plants (Terminalia arjuna). These bacteria are capable of antagonizing the bacterial and virus causing diseases to the Tasar silkworm Antheraea mylitta D. Association of this bacteria with tasar food plant and its antagonistic property were not known earlier.
The bacteria has been identified to be a species of Bacillus/Caterosporus and the same is deposited with Institute of Microbial Technology, IMTECH, Chandigarh and accorded no. 4396 dated 09.09 2002.
The bacteria is isolated from the leaf surface, purified in the laboratory through usual microbiological techniques and cultural, physiological and biochemical features of bacteria are studied to standardize the growth and development of the bacteria . The purified bacteria is multiplied in a lab fermentor, harvested in the laboratory and the desired quantity (5ml @ 108-109 cells/ml) is kept in ampoules.
The bacteria is capable of antagonizing other microorganisms and effective against bacterial and viral diseases of tasar insect.
The last step of the process comprises in preparing soil water suspension a day before spraying on the plant leaves. This is prepared by mixing 5kg of soil thoroughly with approximately 10 litres.of water in a container. The soil is preferably the soil on which Arjuna plant is-grown. However, other soils may also be used.
The soil particles are allowed to settle for example: for 12 hrs so as to obtain clean water from the top of the container. The top water, 5 litre in amount, thus obtained is mixed with one ampoule of bacteria to prepare the bacterial suspension, wherein the suspension comprises 105-106 bacteria. Thereafter, the bacterial suspension is sprayed on the leaves along with the worms during silkworm rearing in out door conditions. The amount of soil, water and bacteria may vary at the rate of above mentioned quantities according to the requirement. For the purpose of preventing the diseases, only two spraying is done once each during 2nd and 3rd instar larvae.
It is to be noted that the present invention is susceptible to modifications, adaptations and changes by those skilled in the art. Such variant Embodiments employing the concepts and features of this invention are intended to be within the scope of the present invention, which is further set forth under the following claims:-









We Claim
1 A Process of Preparation of Bacterial Suspension useful for Biological Control of Tasar Silkworm Diseases comprising the steps of:
a) isolation and purification of Brevibacillus laterosporus R-39 from Terminalia arjuna leaf, which is potent antagonistic bacteria against the pathogenic microorganisms of tasar silkworm Antheraea mylitta D.
b) mass multiplication of purified leaf bacteria characterized in that the important parameters comprising of:
Inoculum dose (104 cell/ml);
growth temperature (35+2°C);
pH of 6.8 to 7.2 for bacterial growth;
Time 36-42 hrs for cell multiplication and harvesting;
Bacterial growth medium consisting of Tryptone 10gm; yeast
extract 5gm; sodium chloride 5g/ litre of dislitted water, at pH
7.0;
c) harvesting of live bacterial mass;
d) its storage in ampules with the critical concentration of bacterial suspension 108-109 cells/ml;
e) concentration of bacteria for foliar spray, wherein critical concentration is of 105-106 cells/ml, using soil water suspension as carrier.
2. A process of Preparation of Bacterial Suspension useful for biological control of Tasar Silkworm diseases, as claimed in Claim 1, wherein Brevibacillus laterosporus R-39 bacteria is having antagonistic property to Tasar silkworm pathogens, namely Serratia marcescens, Proteus vulgaris and cytoplasmic polyhedrosis virus.
3. A process of Preparation of Bacterial Suspension useful for biological control of Tasar silkworm disease, as claimed in Claim 1(e), wherein the soil water suspension, comprises of nutritional ingredients and having pH 5.0 to pH 7.0 and temperature of 22°C-37°C depending upon climatic conditions of tropical sericulture regions.
4. A process of Preparation of Bacterial Suspension useful for biological control of Tasar silkworm diseases, as claimed in Claim 1(e), wherein soil water suspension, for foliar spray, is obtained by mixing 4-5 kg of soil in 8-10 liter of water in a container which is then allowed to settle for 12-15 hours and subsequently taking clean upper water and finally mixing it further with antagonistic bacteria to make the concentration of soil water to 105-106 bacteria per ml.
5. A process of Preparation of Bacterial Suspension useful for biological control of Tasar silkworm diseases as herein described.

Documents:

3051-DEL-2005-Abstract-(07-03-2011).pdf

3051-DEL-2005-Abstract-(24-02-2011).pdf

3051-del-2005-abstract.pdf

3051-del-2005-assignment.pdf

3051-DEL-2005-Claims-(07-03-2011).pdf

3051-DEL-2005-Claims-(24-02-2011).pdf

3051-DEL-2005-Claims-(28-07-2010).pdf

3051-del-2005-claims.pdf

3051-DEL-2005-Correspondence Others-(30-03-2011).pdf

3051-DEL-2005-Correspondence-Others-(07-03-2011).pdf

3051-DEL-2005-Correspondence-Others-(11-04-2011).pdf

3051-DEL-2005-Correspondence-Others-(12-07-2010).pdf

3051-DEL-2005-Correspondence-Others-(24-02-2011).pdf

3051-DEL-2005-Correspondence-Others-(28-07-2010).pdf

3051-DEL-2005-Correspondence-Others-(31-12-2009).pdf

3051-del-2005-correspondence-others.pdf

3051-del-2005-correspondence-po.pdf

3051-DEL-2005-Description (Complete)-(07-03-2011).pdf

3051-del-2005-description (complete).pdf

3051-DEL-2005-Form-1-(07-03-2011).pdf

3051-DEL-2005-Form-1-(28-07-2010).pdf

3051-del-2005-form-1.pdf

3051-del-2005-form-13.pdf

3051-del-2005-form-18.pdf

3051-DEL-2005-Form-2-(07-03-2011).pdf

3051-del-2005-form-2.pdf

3051-DEL-2005-Form-3-(28-07-2010).pdf

3051-DEL-2005-GPA-(12-07-2010).pdf

3051-DEL-2005-Petition-137-(31-12-2009).pdf


Patent Number 247635
Indian Patent Application Number 3051/DEL/2005
PG Journal Number 18/2011
Publication Date 06-May-2011
Grant Date 28-Apr-2011
Date of Filing 16-Nov-2005
Name of Patentee CENTRAL TASAR RESEARCH AND TRAINING INSTITUTE
Applicant Address REGIONAL OFFICE, CENTRAL SILK BOARD, 342-347, 2ND FLOOR, A-WING WEST KRANTI BHAWAN, AUGUST KRANTI BHAWAN, BIKAJI CAMA PLACE, NEW DELHI-110066.
Inventors:
# Inventor's Name Inventor's Address
1 DIPAK KUMAR ROY CENTRAL TASAR RESEARCH & TRANINING INSTITUTE, POST PISKA NAGRI.
2 ALOK SAHAY CENTRAL TASAR RESEARCH & TRAINING INSTITUTE, POST PISKA NAGRI.
3 GAJENDRA PAL SINGH CENTRAL TASAR RESEARCH & TRAINING INSTITUTE, POST PISKA NAGRI.
4 N.SURYANARAYANA CENTRAL TASAR RESEARCH & TRAINING INSTITUTE, POST PISKA NAGRI.
5 D.N. SAHAY CENTRAL TASAR RESEARCH & TRAINING INSTITUTE, POST PISKA NAGRI.
PCT International Classification Number A01N 68/00
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA