Title of Invention

HERBAL SKIN-CARE COMPOSITION

Abstract The present invention discloses herbal skin care compositions for topical application containing extracts from Swertia decusatta (Kadu), Eclipta Alba (Bhringaraj), Santalum album (Sandalwood), Parmelia species (Dagadphool), Ginkgo biloba (Ginkgo, maidenhair tree), Bacopa monnieri (Brahmi), Centella asiatica Mandukpami) and formulations for skin lightening and anti aging creams thereof. Further the present invention discloses preparation of extracts and evaluated for their dermatological effects. In vitro screening of the said herbal extracts for cosmetic applications are carried out for their anti elastase activity, anti hyaluronidase activity, superoxide scavenging activity and anti tyrosinase activity.
Full Text FORM 2
THE PATENTS ACT, 1970
(39 of 1970)
COMPLETE SPECIFICATION
[See section 10, rule 13]
'HERBAL SKIN- CARE COMPOSITIONS"
(a) THE KELKAR EDUCATION TRUST'S SCIENTIFIC RESEARCH CENTRE
(b) S.H.KELKAR & CO. LTD., LAL BAHADUR SHASTRI MARG, MULUND WEST, MUMBAI-400080, India.
(c) Indian company incorporated under the Companies Act 1956
The following specification describes the nature of this invention and the manner in which it is to be performed:




Technical Field of the Invention:
The present invention relates to herbal skin care compositions for topical application comprises extracts from Swertia decusatta (Kadu), Eclipta Alba (Bhringaraj), Santalum album (Sandalwood), Parmelia species (Dagadphool), Adhatoda vasica (Adulsa, vasaka), Ginkgo biloba (Ginkgo, maidenhair tree), Bacopa monnieri (Brahmi) and Centella asiatica (Mandukparni). Further these herbal extracts are evaluated for their dermato logical effects. More particularly, the present invention relates to herbal skin care compositions of anti aging and skin lightening cream for topical application containing the said herbal extracts in addition to per se known adjuvants and expedients.
Background and Prior Art:
Skin pigmentation, age spots - these harmless skin defects can be quite an annoying problem. We all want not only silky smooth complexion, but an even and healthy skin tone as well.
Skin pigmentation, age spots have several things in common. Apart from being hereditary, they are more profound in sun-exposed areas: face, hand, chest and back. These unwelcome brown patches are nothing more than accumulation- of skin pigment melanin, which is always produced by the skin. The amount of melanin present determines the skin tone of an individual. In response to sun exposure the body produces extra melanin: it absorbs UV rays and does not allow them to penetrate deep into the skin layers, as it would be damaging. These harmful UV rays are believed to be the most important extrinsic factor for skin ageing.
Since pigmentation and formation of wrinkles are seen, in the uppermost layer of the skin you might want to consider skin lightening and anti aging Skin care compositions.
Skin care compositions comprising compounds, which inhibit the enzyme tyrosinase, act as skin-whitening agents. Skin care compositions comprising compounds, which act as elastase and hyaluronidase inhibitors and free radical scavengers, can be used as agents to prevent skin aging.
Anti aging cream
From cosmetic agents, the consumer also expects specific effects, such as the prevention of premature skin aging, the avoiding or delaying of wrinkle formation, or effective protection
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against drying of the skin. In the field of herbal cosmetics, there are great opportunities of achieving the above-mentioned effects without appreciable risks to health, since tolerance thereof is sufficiently proven from the tradition of folk medicine in all-important cultures. In particular, the activity of herbal extracts is based on the fact that; in many cases, the active substances from plants have also been used as pure substances. There are also cases, however, where the components of an extract display their activity only as a whole. But naturals may not mean safe, so it is expected that they should be tested for safety to human skin.
Skin lightening cream
A fair skin tone in some culture continues to be the foremost indicator of beauty and high social status. So, most people are concerned with the degree of their skin's pigmentation and desire a light skin tone without spots or dull appearance. The proposed skin lightening cream works by the tyrosinase inhibition mechanism which is regarded safe and unlike bleaching agents is non carcinogenic.
Methods for reducing cellulite in mammalian skin is disclosed in US Pat.5, 962,482, US Pat.5, 854,046 and US Pat.5, 958,750. US Pat. 6,057,110 describe human hyaluronidase. Therapeutic and cosmetic compositions for treatment of skin are claimed in US Pat.5, 538,740.
Japanese patent JP2003238434 describes hyaluronidase activity inhibitor comprising an extract of Hizikia fusiforme or Corcharus oltorius and provide food and beverage and cosmetic containing the same.
Other Japanese patent JP7157420 discloses a cosmetic having effectiveness capable of being provided with improved whitening, ant oxidation and hyaluronidase activity inhibiting effect in addition to an advantage in appearance and an improved moist holding property by cholesteric liquid crystals. This cosmetic comprises a cholesteric liquid crystal compounded with >=1 extract of Adhatoda vasica, Lagerstroemia speciosa, Woodfordia fruticosa, Cymbopogon nardus, Cardiospermum halicacabum, Vetiveria zizanoides, Piper longum, Michelia champaca, Hemidesmus indicus, Piper chaba, Tinospora cordifolia, Melaleuca leucadendron, Azadirachdt indica, Murraya koenigii, Sphaeranthus indicus, Phyllanthus nuriri, Desmodium gangeticum and Smilax zeylanica.
Even though there are various prior arts available on skin care compositions, there is no relevant prior art patent available on skin care compositions of these herbal extracts.
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In the present invention, herbal extracts from a wide variety of plants have been prepared and their dermatological effects have been evaluated. Further, it has been observed that the plant extracts and the compositions thereof have therapeutic and cosmetic effects on human skin. Antielastase and antihyaluromidase activity of the compositions of herbal extracts are described in this invention. It is further observed that these compositions also possess free radical scavenging activity. These compositions are useful to prevent skin aging. Present compositions of plant extracts inhibit the enzyme tyrosinase and hence are effective skin whitening and skin lightening agents.
Summary of the Invention:
The present invention discloses herbal skin care compositions for topical application containing extracts from Swertia decusatta (Kadu), Eclipta Alba (Bhringaraj), Santalum album (Sandalwood), Parmelia species (Dagadphool), Ginkgo biloba (Ginkgo, maidenhair tree), Bacopa monnieri (Brahmi), Centella asiatica (Mandukpami) and formulations for skin lightening and anti aging creams thereof. Further the present invention discloses preparation of extracts and evaluated for their dermatological effects. In vitro screening of the said herbal extracts for cosmetic applications are carried out for their anti elastase activity, anti hyaluronidase activity, superoxide scavenging activity and anti tyrosinase activity.
Detailed Description:
The skin compositions of the following herbal origins have been prepared. The herbal extracts are evaluated for their anti elastase anti hyaluronidase activities, their free-radical scavenging activity and their anti tyrosinase activity and claimed in this invention. The herbal extracts compositions of Swertia decusatta (Kadu), Eclipta Alba (Bhringaraj), Santalum album (Sandalwood), Parmelia species (Dagadphool), Adhatoda vasica (Adulsa, vasaka), Ginkgo biloba (Ginkgo, maidenhair tree), Bacopa monnieri (Brahmi), Centella asiatica (Mandukpami), have been prepared and medicinal and cosmetic properties of these herbal extracts are valuated.
Preparation of extracts
Extract of Eclipta alba is the 70% alcoholic extract from shade dried leaves powdered to mesh # 40, that is filtered before vacuum drying.
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Extract Centella asiatica is obtained from shade dried leaves powdered to mesh # 20 and which are subjected to reflux with 60% v/v alcohol at a temperature not exceeding 80°C throughout extraction. The process continued for 48 hrs after which the extracts were cooled, filtered and vacuum dried.
Santalum album extract is prepared by cold extraction of dried paste of the heartwood of santalum album with 60%v/v alcohol.
Extract of Bacopa Monnieri is obtained from shade dried leaves powdered to mesh # 20 and which are subjected to reflux at temperature not exceeding 80°C throughout extraction. The process continued for approx. 48 hrs after which the extracts were cooled, filtered and vacuum dried.
Swertia decussata extract is obtained by refluxing the powdered shade dried leaves mesh # 20 with 50% v/v methanol in a water bath for 4 hrs. The extract so obtained is then filtered and vacuum dried.
Parmelia species is obtained from shade dried flowers powdered to mesh # 40 and which are subjected to reflux with 60% v/v alcohol at a temperature not exceeding 80°C throughout extraction. The process continued for 48 hrs after which the extracts were cooled, filtered and vacuum dried.
Ginkgo biloba extract is prepared by reflux condensation of shade dried leaves reduced to mesh#40, with 70%v/v alcohol. The extract so obtained is filtered and dried under vacuum.
The extracts mentioned above are screened for anti aging and skin lightening activities with suitable vehicles/ carriers. The anti aging activity of herbal extracts is found by three major properties anti oxidant (superoxide scavenging / free radical scavenging), anti elastase (Elastin is the important protein of dermis that is responsible for firmness. Elastase is the lysozymal enzyme that promotes aging by degrading the elastin), anti hyaluronidase (Hyaluronic acid/ hyaluronan is the glycosaminoglycan known for its water holding capacity. Hyaluronidase enzyme is responsible for the pharmacological control on Hyaluronic acid turnover in skin and thus promotes aging). Similarly, anti tyrosinase activity is a criterion for proposing skin lightening activity of herbal extracts.
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In vitro screening of the said herbal extracts for cosmetic applications
Anti Elastase activity- Porcine pancreatic elastase (PPE: sigma Aldrich, type IV) was assayed spectrophotometrically by the modified method of James et al, remodified by K.K. Lee, J.H. Kim, A. J. Cho, and J.D. Choi, using N-Succ-(Ala )3- p-nitroanilide as a substrate, and monitoring the release of p- nitroaniline for 20 min. at 25°C and 410 nm. Reconstitution of the dried herbal extract was done in distilled water.
Anti Hyaluronidase activity- Hyaluronidase activity was determined spectrophotometrically by measuring the amount of N- acetylglucosamine at an optical density of 585nm formed from sodium hyaluronate. Reconstitution of the dried herbal extract was done in 5% DMSO.
Superoxide scavenging activity- The superoxide scavenging ability of above mentioned herbal extracts was measured spectrophotometrically at 560nm. By quantification of difarmazan released as a result of reduction of Nitroblue tetrazolium by NADH and PMS acting as a catalyst. Reconstitution of the dried herbal extract was done in distilled water.
Anti Tyrosinase activity- The anti tyrosinase activity of herbal extracts was determined by pre-incubating the herbal extract to be screened with enzyme tyrosinase from mushroom (Sigma Aldrich). The substrate L Tyrosine is the added and the mixture is incubated at 37°C. The quantification of DOPA quionone released is done spectrophotometrically at 475 nm. Reconstitution of the dried herbal extract was done in 70% ethanol.
Of the above mentioned herbal extracts following herbs showed skin anti aging and skin lightening activities which are tabulated in the Table 1.
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Table 1 : skin anti aging and skin lightening activities for the herbal extracts

Anti Aging Anti Aging Skin Lightening
Herbal Extract Superoxide scavenging Anti hyaluronidase Anti elastase Anti Tyrosinase
Eclipta alba 5mg/ml 62.83 64.68 74.38 -
Centellaasiatica5mg/ml 37.20 58.79
Bacopamonnieri5mg/ml 51.30 57.79
SweretiadecussataSmg/ml 79.14
Parmelia sp. 5mg/ml - . - - 84.00
Santalumalbum5mg/ml 52.34 55.00
Ginkgobiloba5mg/ml 95.00
Compounds, which act as elastase and hyaluronidase inhibitors and free radical scavengers, can be used as agents to prevent skin aging. Compounds, which inhibit the enzyme tyrosinase, act as skin-whitening agents.
Anti aging cream
From cosmetic agents, the consumer also expects specific effects, such as the prevention of premature skin aging, the avoiding or delaying of wrinkle formation, or effective protection against drying of the skin. In the field of herbal cosmetics, there are great opportunities of achieving the above-mentioned effects without appreciable risks to health, since tolerance thereof is sufficiently proven from the tradition of folk medicine in all- important cultures. In particular, the activity of herbal extracts is based on that fact; in many cases, the active substances from plants have also been used as pure substances. There are also cases, however, where the components of an extract display their activity only as a whole. But naturals may not mean safe, so it is expected that they should be tested for safety to human skin.
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It has been found from in vitro screening of herbal extracts for their anti aging properties that the claimed combination of the extracts, when used as active ingredients in skin and body care preparations, has a special cosmetic and care-providing property which results in the reduction of wrinkle depth and increase of skin elasticity.
"Extract" within the meaning of the present invention comprises an extract from the plant parts mentioned as obtained by means of a solvent, optionally under pressure and / or heat. The quality and quantity of the composition of active substances may slightly vary depending on the extractant and extraction method employed
Depending on the cosmetic activities screened a herbal skin care composition for anti aging property, herbal extracts with more than 50% inhibition activity is proposed to be incorporated
In a preferred embodiment of the present invention, the formulations contain from 0.01 to 4% of herbal extracts (w/w), especially from 0.025- 2% extracts of Eclipta alba (w/w), from 0.025-1.5% extracts of Centella asiatica (w/w) and 0.025- 1.5% extract of Bacopa monnieri (w/w), from 0.1- 1% extract of Ginkgo biloba (w/w) and from 0.01-2% extract of santalum album (w/w). If too low a concentration of use is selected, the desired effect will not be achieved. However, if the amounts of extracts are adjusted too high, an economical exploitation is not possible. In addition, considerable galenic problems result in the processing of extracts in the cosmetic formulations.
The present formulation is of semi solid oil in water emulsion preferably light creams and lotions, which consists of the mentioned plant extracts only. The latter as claimed by us to have anti oxidant {Eclipta alba, Centella asiatica, Bacopa monnieri), hyaluronidase inhibition activity {Eclipta alba, Centella asiatica, Bacopa monnieri) and anti elastase activity {Ginkgo biloba, Eclipta alba, Santalum album). Preferably, the formulations would be free from colorants and preservatives, should quickly penetrate into the skin and not impart a greasy feel.
(a) An extract from of, Eclipta Alba (Bhringaraj), Bacopa monnieri (Brahmi), Centella asiatica (Mandukparni);
(b) An extract from Ginkgo biloba, Santalum album
(c) A cosmetically acceptable vehicle for topical administration.
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The formulation comprising 0.1- 4 % extract of Eclipta alba (w/w), and 0.05 to 3% extracts of Centella asiatica (w/w) and 0.05- 1.5% extract of Bacopa monnieri (w/w), 0.1- 1% extract of Ginkgo biloba (w/w), 0.01-2.0% extract of Santalum album (w/w).
The herbal skin care composition comprises cosmetically acceptable vehicle selected from the group consisting of vegetable oils, alcohols, emulsifiers, humectants, antioxidants, thickeners, perfumes and / or mixtures thereof.
Skin lightening cream
A fair skin tone in some culture continues to be the foremost indicator of beauty and high social status. So, most people are concerned with the degree of their skin's pigmentation and desire a light skin tone without spots or dull appearance. The proposed skin lightening cream works by the tyrosinase inhibition mechanism which is regarded safe and unlike bleaching agents is non carcinogenic.
It has been found from in vitro screening of herbal extracts for their anti aging properties that the claimed combination of the extracts, when used as active ingredients in skin and body care preparations, has a special cosmetic and care-providing property which results in reduction of wrinkle depth and increase of skin elasticity.
"Extract" within the meaning of the present invention comprises an extract from the plant parts mentioned as obtained by means of a solvent, optionally under pressure and / or heat. The quality and quantity of the composition of active substances
Depending on the cosmetic activities screened, a herbal skin care composition for skin lightening property with herbal extracts having more than 50% tyrosinase inhibition activity is prepared.
In a preferred embodiment of the present invention, the formulations comprises 0.01 to 5 % herbal extracts (w/w), preferably 0.025- 2 % herbal extracts (w/w), 0.025-5.0 % of Sweretia decussata (w/w) and from 0.005- 3.0 % extract of Parmeliaa sp. (w/w) and 0.050- 3.0 % Santalum album(w/w). If too low a concentration of use is selected, the desired effect will not be achieved.
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However, if the amounts of extracts are adjusted too high, an economical exploitation is not possible.
The formulation is of a semi solid oil in water emulsion preferably light creams and lotions, which consists of the mentioned plant extracts only. The latter have anti oxidant, hyaluronidase inhibition activity and promote the regeneration of the skin. Preferably, the formulations is free from colorants and preservatives should quickly penetrate into the skin and not impart a greasy feel.
(a) An extract from of, Sweretia decussata, Parmelia sp, Santalum album;
(b) A cosmetically acceptable vehicle for topical administration.
The formulation comprising of 0.025-5.0% of Sweretia decussata and 0.005- 3.0% by weight of Parmelia sp. and 0.050- 3.0% Santalum album.
The formulation comprises the cosmetically acceptable vehicle is selected from the group consisting of vegetable oils, alcohols, emulsifiers, humectants, antioxidants, thickeners, perfumes, and or mixtures thereof. The skin lightening composition would especially comprise of a combination of UV filters.
Cosmetically acceptable vehicle
Oil-in water emulsions on the basis of non-ionic emulsifiers:
Oil in water emulsion in semisolid form was selected over water in oil emulsion to act as a suitable vehicle for the incorporation of active. Oil in water system is light textured, less greasy and imparts an elegant skin feel best suited for Indian climatic conditions. On the other hand water in oil emulsions being historically associated with a heavy, and greasy skin feel on application (for Indian skin) are less frequently used in skin care products. Above all, oil in water emulsion forms an ideal vehicle for proposed extracts to be incorporated.
Presence of UV Filters (For skin lightening creams)
The composition contained ultraviolet rays absorbers or filters that prevent the reversal of skin lightening mediated by the active.
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Raw materials
The oil in water cream composition would form the base for proposed Herbal skin care composition. The base formulation for the incorporation of herbal extracts would be designed on the lines of skin care products commonly employed. The ingredients would be selected on the basis of their inherent properties like usefulness in a skin care composition, compatibility with other components and cost. The brief introduction for the proposed raw materials is given here under:
Emulsifier System
The most important step in producing an emulsion is the selection of a suitable emulsifier. The most commonly used emulsifiers are anionic and nonionic systems, or combinations of the two. The primary selection is based on the emulsion type (oil/water or water/oil) and the emulsifier must be compatible with the oil phase, water phase and the actives used in the formulation. The primary emulsifier is the main determinant factor of the emulsion type, while the secondary emulsifier serves to strengthen the interphase thereby improving its stability. The use of sodium soaps is often rejected, as they are too alkaline. When neutralized they produce a hard soap with limited solubility. Potassium Stearate is one of the most widely used emulsifiers as they are cheap and readily available. But it is irritating to the skin the skin and has a tendency to gel with time. A number of other factors like mildness on skin, color, odor, emollient effect and solubility in both the phases would also be considered while selecting the emulsifier system. The emulsifiers to be incorporated in the herbal skin care compositions are selected from sodium stearate, potassium stearate, PEG 400 stearate, Glyceryl monostearate, Arlacel 165.
Co-emulsifiers
In purely thermodynamic backgrounds, the emulsions are physically unstable and therefore need to be stabilized. In order to maintain the droplets as separate entities, and to prevent the phases from separating, accessory emulsifiers or "co-emulsifiers" are often utilized. These co-emulsifiers prevent the oil phase from coalescing or creaming and keep the phases physically stable as an emulsion prior to application to the skin. They lend "body" to the emulsion and give the formulation its character as a lotion or a cream by imparting viscosity to the composition. Fatty alcohols used here, such as cetyl and stearyl alcohols are particularly useful as co-emulsifiers as 2: lor 1:2 and between 1-5% by weight.
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Emollients
The herbal skin care composition would be a skin cream whose performance would be evaluated in terms of the prevention of relief from dryness, ease of spreading, skin feel as well as protection. The combination of these parameters is often termed as emolliency. A combination of Stearic Acid (Triple pressed) 0.1-2%, Isopropyl Myristate0.05-10%, Iso propyl palmitate 0.05-2.5%, Cocoa ButterO.025-15%, Olive Oil 0.001- 50%, almond oil 0.1- 10% (oil soluble emollients), and Glycerol 0.05-20%, sorbitol 0.01- 10%, propylene glycol 0.05-10% (a water soluble humectant) would be used in the proposed skin care composition.
Silicone oils or fluids
Silicone oils or fluids are used to improve the composition's lubricant effect on application to the skin. Silicone oils or fluids proposed to be used are dimethylpolysiloxane, diethylpolysiloxane, diphenylpolysiloxane. Silicone Oils are represented by R.2 [Si-0]n. The silicone fluids would be used in the range 0.002-2%.
Viscosity modifiers
The oil in water emulsions are generally of thin consistency. The formulation of skin cream proposed requires a cream base of suitable consistency. The viscosity of such emulsions would be easily adjusted using the easiest to used homo polymer of acrylic acid, sold under the trade name Carbopol 940, Carbopol Ultrez 10. These new unique polymer would be sufficient to impart high microgel particle rigidity as well as high thixotropy required for formulating a wide variety of products. The thickeners with low dispersion property as well as minimum neutralization requirement would be selected.
pH Regulators
In most liquid systems, Carbopol polymers require neutralization to thicken most efficiently. Unneutralized dispersions have an approximate pH range of 2.5-3.5 depending on the polymer concentration. Once a neutralizer is added to the dispersion, thickening gradually occurs. Optimum neutralization is achieved at a pH of 6.5-7.0. High viscosities would be achieved in a range of 5.0-9.0. The neutralizers would be selected from Sodium hydroxide, potassium hydroxide, ammonium hydroxide, and some water-soluble organic amines which are excellent neutralizing agents for Carbopol polymers in water systems agent. The usage range would be between 0.001- 2.00% by weight. The usage range would be between 0.001- 2.00% by weight.
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Ultra Violet light filters (for skin lightening creams)
Ultra Violet light filters are ingredients that absorb, reflect, scatter and block UV rays which are harmful to the skin. UV filters are used as stabilizers, to protect the integrity of the finished products. In skin lightening creams it would be a must to include a combination of UVA and UVB filters or sunscreens to prevent the skin from an unwanted tan and sunspots. UVA and UVB filters are selected from cinnamates, benzophenones, PABA derivatives, salicylates, physical blockers like Ti02, ZnO, Dibenzoyl methane. A combination of UVA AND UVB filters would be the most suitable to give excellent broad spectrum protection.
Demineralised Water
With the exception of oils or oily products, in almost all other cosmetic products, the main ingredient is water. In creams, lotions, shampoos or conditioners water is the largest ingredient making up 30% to 99.9% of the weight of the product.
The claimed "herbal extracts", range from 0.001 to 35% of the product. The moisturizing, emulsifying, supporting, stabilizing, preserving, thickening and other ingredients make the 100% full. The water in the proposed herbal skin care composition would be of high quality to make a high quality cosmetically acceptable vehicle for the claimed herbal extracts.
Preservatives
Preservatives are used to retard microbial (bacterial and fungal) contamination of cosmetics. The high water content of many cosmetics makes them suitable places for microbial growth. Combinations of preservatives are frequently used. The preservatives would include a combination of any 2-5 preservatives from the list - methyl paraben, propyl paraben, butyl paraben, Quaternium-8, -14 or -15, imidazolidinyl urea, diazolidinyl urea, phenoxy ethanol, citric acid and ethanol.
Disodium EDTA
It is Ethylene diamine tetra acetic acid disodium salt C10H14N2O8Na2. This is a water soluble chelating agent because of its characteristic of neutralization. It has the property of surrounding a metal, such as ca or Mg, to form very stable complexes that help to keep such metals away from
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the skin and products, preventing them from supporting any growth of microorganisms. Prevents product deterioration
Perfume
Fragrance is a significant part of creams. Perfume is not only added to give products a pleasant fragrance, but is often added to disguise the smell of malodorous ingredients like fats and oils or an ingredient that is considered unpleasant. Perfumes are used in very small concentrations. It is important to keep the perfume light so that the products feel clean and fresh on the skin rather than heavy. The perfume is used in the range 0.001- 5% . The herbal skin care composition proposed would contain a minimum quantity of perfumes just sufficient to mask the base cream raw material's odour.
The following examples describe the invention and are in no way limiting the scope of the invention.
Example 1
Formulation of herbal skin care composition for Anti Aging or Age Defying Cream
Extracts to be used for their anti aging properties
An extract from Eclipta Alba (Bhringaraj) - Free radical scavenging, anti hyaluronidase and anti
elastase activity;
Bacopa monnieri (Brahmi), Centella asiatica (Mandukparni) - Free radical and anti
hyaluronidase activity;
An extract from Ginkgo biloba, Santalum album - Anti Elastase activity
The formulation comprising 0.1- 4 % by weight of the extract from Eclipta alba, and 0.05 to 3% by weight of the extracts from Centella asiatica and Bacopa monnieri 0.05- 1.5%, from 0.1-1% Ginkgo biloba, from 0.01-2.0% Santalum album.

S.NO INGREDIENTS %W/W [Fill
A) Aqueous Phase
1 Propylene glycol 0.05-10%
2 Dl-Sodium EDTA 0.001-2.0%
3 Carbomer 0.001-10%
4 NaOH 0.001-10%
6 Water (demineralized) UPTO 100
B) Oil Phase
7 GMS (SE) 0.01-20%
8 Cetyl Alcohol 0.2- 4%
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9 Stearic acid 0.1-30%
10 Isopropyl Palmitate 0.05-13%
12 Almond oil 0.1-15%
13 Cocoa butter 0.01-10%
16 Silicon fluid 0. 02-10%
C) Preservatives
17 Phenoxy ethanol, parabens 0.10-2%
D) Perfume 0.001-2%
E) Extracts 0.01-4%
Example 2
Formulation of herbal skin care composition for Skin Lightening Cream
Extracts to be used for their anti tyrosinase activity
The formulations would contain from 0.01 to 5% by weight of the extracts, especially from 0.025- 2% by weight of the extracts, 0.025-5.0% of Sweretia decussata and from 0.005- 3.0% by weight of Parmelia sp. and 0.050- 3.0% Santalum album.

S.NO INGREDIENTS %W/W
A) Aqueous Phase
1 Sorbitol 0.05-10%
2 Dl-Sodium EDTA 0.001-2.0%
3 Carbomer 940 0.001-10%
4 NaOH 0.001-10%
6 Water (demineralized) UPTO100
B) Oil Phase
7 GMS (SE) 0.01-20%
8 Cetyl alcohol 0.2- 4%
9 Stearic acid 0.1-30%
10 Isopropyl Palmitate 0.05-13%
12 Olive oil 0.1-15%
13 Cocoa butter 0.01-10%
14 Siliconfluid 0. 02-10%
C) Sunscreens
15. PABA derivatives 0.05-5%
D) Preservatives
17 Phenoxy ethanol, parabens 0.10-2%
E) Perfume 0.001-2%
F) Extracts 0.01-5%
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While the present invention is described above in connection with preferred or illustrative embodiments, these embodiments are not intended to be exhaustive or limiting of the invention. Rather, the invention is intended to cover all alternatives, modifications and equivalents included within its scope, as defined by the appended claims.
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We claim,
1. Herbal skin care compositions for anti aging and skin lightening creams characterized in that the said herbal anti aging composition comprises
a) 0.01-4 % herbal extracts (w/w),
b) aqueous Phase comprising of water soluble humectant such as propylene glycol 0.05-10%, chelating agent such as di-Sodium EDTA 0.001- 2.0%, thickener such as Carbomer 0.001-10%, neutralizer such as sodium hydroxide 0.001-10% and water (demineralized) UPTO 100;
c) oil phase comprising of self emulsifying agent such as GMS (SE) 0.01-20%; co-emulsifier such as Cetyl Alcohol 0.2- 4%, emollients such as Stearic acid 0.1-30%, Isopropyl Palmitate 0.05-13%, Almond oil 0.1-15%, Cocoa butter 0.01-10%, Silicon fluid 0. 02-10%;
d) preservatives comprising Phenoxy ethanol, parabens 0.10- 2%; and
e) perfume 0.001-2%;
further characterized in that the said herbal skin lightening creams comprises
a) 0.01 to 5 % of herbal extracts (w/w), preferably 0.025- 2 % extracts,
b) aqueous Phase comprises water soluble humectant such as Sorbitol 0.05-10%, chelating agent such as Di-Sodium EDTA 0.001- 2.0%, thickener such as Carbomer 940 0.001-10%, neutralizer such as sodium hydroxide 0.001-10%, Water (demineralized) UPTO 100;
c) oil Phase comprises self emulsifying agent such as GMS (SE) 0.01- 20%, co-emulsifier such as Cetyl alcohol 0.2- 4%, emollient such as Stearic acid 0.1-30%, Isopropyl Palmitate 0.05-13%, Olive oil 0.1-15%, Cocoa butter 0.01-10%, Silicon fluid 0. 02-10%;
d) sunscreens comprising PABA derivatives 0.05- 5%;
e) preservatives comprising Phenoxy ethanol, parabens 0.10- 2%; and
f) perfume comprising 0.001-2%.
2. Herbal skin care compositions for anti aging as claimed in claim 1 wherein the said herbal extract comprises 0.025 - 4 % of Eclipta alba extract (w/w), preferably 0.025 -2%; 0.025-3 % of Centella asiatica extract (w/w), preferably 0.025-1.5 %; 0.025 -1.5 %
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of Bacopa monnieri extract (w/w), 0.1-1 % of Ginkgo biloba extract (w/w) and 0.01-2 % of Santalum album extract (w/w).
3. Herbal skin care compositions for skin lightening cream as claimed in claim 1 wherein the said herbal extract comprises 0.025-5.0% of Sweretia decussate extract (w/w) and 0.005- 3.0 % of Parmelia sp. and 0.050- 3.0 % Santalum album.
4. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Eclipta alba is the 70% alcoholic extract from shade dried leaves powdered to mesh # 40, that is filtered before vacuum drying.
5. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Centella asiatica is prepared from shade dried leaves powdered to mesh # 20 and which are subjected to reflux with 60% v/v alcohol at a temperature not exceeding 80°C throughout extraction for 48 hrs after, cooled the said extracts, filtered and vacuum dried.
6. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Santalum album is prepared by cold extraction of dried paste of the heartwood of Santalum album with 60%v/v alcohol.
7. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Bacopa Monnieri is prepared from shade dried leaves powdered to mesh # 20 and which are subjected to reflux at temperature not exceeding 80°C throughout extraction for approx. 48 hrs after, cooled the said extract, filtered and vacuum dried.
8. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Swertia decussata is prepared by refluxing the powdered shade dried leaves mesh # 20 with 50% v/v methanol in a water bath for 4 hrs, filtered the said extract and vacuum dried.
9. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Parmelia species is prepared from shade dried flowers powdered to mesh # 40 and which are subjected to reflux with 60% v/v alcohol at a temperature not exceeding 80°C throughout extraction for 48 hrs after, cooled the said extract, filtered and vacuum dried.
10. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 wherein the said extract of Ginkgo biloba is prepared by reflux
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condensation of shade dreid leaves reduced to mesh#40, with 70%v/v alcohol, filtered the said extract and dried under vacuum.
11. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 4 wherein the said extract of Eclipta alba extract have Anti aging activity such as superoxide scavenging activity 62.83 %, Anti hyaluronidase activity 64.68 % and Anti elastase activity 74.38 %.
12. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 and 5 wherein the said extract of Centella asiatica have Anti aging activity Superoxide scavenging activity 37.20 and Anti hyaluronidase 58.79.
13. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 and 6 wherein the said extract of Santalum album have Anti aging activity such as Anti elastase 52.34% and Skin lightening activity such as Anti Tyrosinase 55.00%.
14. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 and 7 wherein the said extract of Bacopa Monnieri have Anti Aging activity such as Superoxide scavenging 51.30% and Anti hyaluronidase 57.79.
15. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 and 8 wherein the said extract of Swertia decussata has skin lightening activity such as Anti Tyrosinase 79.14 %.
16. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 and 9 wherein the said extract of Parmelia species has skin lightening activity such as Anti-Tyrosinase 84.00 %.
17. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3 and 10 wherein the said extract of Ginkgo biloba has Anti aging activity such as Anti elastase 95.00 %.
18. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 4 and 11 wherein the said Anti aging activity such as superoxide scavenging activity 62.83 %, Anti hyaluronidase activity 64.68 % and Anti elastase activity 74.38 % of the said extract of Eclipta alba is for 5 mg/ml concentration of the said Eclipta alba extract.
19. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 5 and 12 wherein the said Anti aging activity such as Superoxide scavenging activity 37.20 and Anti hyaluronidase 58.79 of the said extract of Centella asiatica is for 5 mg /ml concentration of the said Centella asiatica extract.
19

20. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 6 and 13 wherein the said Anti aging activity such as Anti elastase 52.34% and Skin lightening activity such as Anti Tyrosinase 55.00% of the said extract of Santalum album is for 5 mg/ml concentration of the said Santalum album extract.
21. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 7 and 14 wherein the said Anti Aging activity such as Superoxide scavenging 51.30% and Anti hyaluronidase 57.79 of the said extract of Bacopa Monnieri is for 5 mg / ml concentration of the said Bacopa Monnieri extrcat.
22. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 8 and 15 wherein the said skin lightening activity such as Anti Tyrosinase 79.14 % of the said extract of Swertia decussata is for 5 mg / ml concentration of the said Swertia decussata extract.
23. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 9 and 16 wherein the said skin lightening activity such as Anti-Tyrosinase 84.00 % of the said extract of Parmelia species is for 5 mg/ ml concentration of the said Parmelia species extract. ?
24. Herbal skin care compositions for anti aging and skin lightening cream as claimed in claims 1 to 3, 10 and 17 wherein the said Anti aging activity such as Anti elastase 95.00 % of the said extract of Ginkgo biloba is for 5 mg /ml concentration of the said Ginkgo biloba extract.
25. Herbal skin care compositions for anti aging and skin lightening creams as substantially described herein with reference to foregoing examples 1 to 2.


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Abstract:
The present invention discloses herbal skin care compositions for topical application containing extracts from Swertia decusatta (Kadu), Eclipta Alba (Bhringaraj), Santalum album (Sandalwood), Parmelia species (Dagadphool), Ginkgo biloba (Ginkgo, maidenhair tree), Bacopa monnieri (Brahmi), Centella asiatica Mandukpami) and formulations for skin lightening and anti aging creams thereof. Further the present invention discloses preparation of extracts and evaluated for their dermatological effects. In vitro screening of the said herbal extracts for cosmetic applications are carried out for their anti elastase activity, anti hyaluronidase activity, superoxide scavenging activity and anti tyrosinase activity.

Documents:

233-mum-2003-abstract (complete).doc

233-mum-2003-abstract (complete).pdf

233-mum-2003-abstract(13-7-2007).pdf

233-MUM-2003-ABSTRACT(AMENDED)-(25-4-2011).pdf

233-MUM-2003-ANNEXTURE(3-2-2010).pdf

233-mum-2003-cancelled pages(13-7-2007).pdf

233-mum-2003-claims (complete).doc

233-mum-2003-claims (complete).pdf

233-MUM-2003-CLAIMS(GRANTED)-(25-4-2011).pdf

233-mum-2003-correspondence(13-7-2007).pdf

233-MUM-2003-CORRESPONDENCE(3-12-2010).pdf

233-MUM-2003-CORRESPONDENCE(3-2-2010).pdf

233-MUM-2003-CORRESPONDENCE(3-2-2011).pdf

233-mum-2003-correspondence(ipo)-(14-9-2006).pdf

233-MUM-2003-CORRESPONDENCE(IPO)-(25-4-2011).pdf

233-mum-2003-correspondence-received-070305.pdf

233-mum-2003-correspondence-received-090804.pdf

233-mum-2003-correspondence-received-190303.pdf

233-mum-2003-correspondence-received-260204.pdf

233-mum-2003-correspondence-received-270203.pdf

233-mum-2003-correspondence-received.pdf

233-mum-2003-description (complete).pdf

233-mum-2003-description (provisional).pdf

233-MUM-2003-DESCRIPTION(GRANTED)-(25-4-2011).pdf

233-mum-2003-form 3(27-2-2003).pdf

233-mum-2003-form 1(13-7-2007).pdf

233-mum-2003-form 1(19-3-2003).pdf

233-MUM-2003-FORM 19(9-8-2004).pdf

233-MUM-2003-FORM 2(GRANTED)-(25-4-2011).pdf

233-MUM-2003-FORM 2(TITLE PAGE)-(COMPLETE)-(26-2-2004).pdf

233-MUM-2003-FORM 2(TITLE PAGE)-(GRANTED)-(25-4-2011).pdf

233-MUM-2003-FORM 2(TITLE PAGE)-(PROVISIONAL)-(27-2-2003).pdf

233-MUM-2003-FORM 26(28-4-2003).pdf

233-mum-2003-form 3(13-7-2007).pdf

233-MUM-2003-FORM 5(26-2-2004).pdf

233-mum-2003-form-1.pdf

233-mum-2003-form-19.pdf

233-mum-2003-form-2 (complete).doc

233-mum-2003-form-2 (complete).pdf

233-mum-2003-form-2 (digram).doc

233-mum-2003-form-2 (provisional).doc

233-mum-2003-form-2 (provisional).pdf

233-mum-2003-form-26.pdf

233-mum-2003-form-5.pdf

233-mum-2003-specification(amanded)-(13-7-2007).pdf

233-MUM-2003-SPECIFICATION(AMENDED)-(13-7-2007).pdf


Patent Number 247575
Indian Patent Application Number 233/MUM/2003
PG Journal Number 17/2011
Publication Date 29-Apr-2011
Grant Date 25-Apr-2011
Date of Filing 27-Feb-2003
Name of Patentee THE KELKAR EDUCATION TRUST'S SCIENTIFIC RESEARCH CENTRE
Applicant Address S. H. KELKAR & CO. LTD., LAL BAHADUR SHASTRI MARG, MULUND (WEST), MUMBAI-400 080
Inventors:
# Inventor's Name Inventor's Address
1 MAINKAR, A. R. 83 A/44 YASHODA C.H.S, VRINDAVAN SOCIETY, MAJIWADE, THANE 400 601
2 KELKAR, G. D. RESIDENTIAL BLDG., S.H. KELKAR & CO. LTD., LAL BAHADUR SHASTRI MARG, MULUND (WEST), MUMBAI-400 080
3 HEBLE, M. R. C 48, DINDAYAL NAGAR, NSVGHAR ROAD, MULUND (E), MUMBAI-400 081
4 SUNDARAM, S. NO.3, SRINIKETAN, 14TH ROAD, SHEMBUR, MUMBAI-400 071
PCT International Classification Number A61K36/254; A61Q19/00; A61K36/185
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA