Title of Invention

"CIS-1(5-CYANO-PYRIDIN-2-YL)-3-(4,7-DIFLUORO-1,1A,2,7B-TETRAHYDRO CYCLOPROPA[C]CHROMEN-1-YL)-UREA OF FORMULA 1"

Abstract A compound of the formula I: where; R1 is O, S; R2 is an optionally substituted nitrogen-containing heterocycle, wherein the nitrogen is located at the 2 position relative to the (thio) urea bond; R3 is H, C1-C3 alkyl, R4-R7 are independently selected from H, C1-C6 alkyl,C2-C6 alkenyl, C2-C6 alkynyl, haloC1-C6alkyl, C1-C6 alkanoyl, halo C1-C6 alkanoylC1-C6 alkoxy, halo C1-C6 alkoxy, C1-C6 alkyloxy-V alkyl, haloC1-C6 alkyloxy-C1-C6 alkyl hydroxy- C1-C6 alkyl, amino- C1-C6 alkyl, carboxy- C1-C6 alkyl, cyano- C1-C6 alkyl, amino, carboxy, carbamoyl, cyano, halo, hydroxy; X is —(CHR8)n—D—(CHR8)m—; D is —NR9 --, —O—, —S—-, — S(=O)— or —S(=O)2—; R8 is independently H, C1-C3 alkyl, halo substituted C1-C3 alkyl; R9 is H, C1-C3 alkyl; n and m are independently 0, 1 or 2; or prodrugs or pharmaceutically acceptable salts thereof.
Full Text Technical field
This invention relates to non-nucleoside reverse transcriptase inhibitors active
against HIV-1 and having an improved resistance and pharmacokinetic profile.
The invention further relates to novel intermediates in the synthesis of such
compounds and the use of the compounds in antiviral methods and
compositions.
Background to the invention
Non nucleoside reverse transcriptase inhibitors (NNRTI) bind to an allosteric site on reverse transcriptase and represent an important development in the arsenal of drugs against HIV, particularly HIV-1. International patent application WO 93/03022, discloses thiourea NNRTI which were later denoted "PETT" (phenyl ethyl thiazolyl thiourea) compounds in J Med Chem 39 6 1329-1335 (1995) and J Med Chem 39 21 4261-4274 (1996). International patent application nos. WO99/47501, WO/0039095, WO/0056736, WOOO/78315 and WOOO/78721 describe thiourea PETT derivatives which hcn/e allegedly been optimised against a composite RT binding pocket.
International patent application no W095/06034 and J Med Chem 42 4150-4160 (1999) disclose urea isosteres of PETT NNRTIs. International patent application no WO99/36406 discloses urea.NNRTI compounds with a freestanding cyclopropyl bridge, wherein the phenyl left hand wing bears an obligate 6-hydroxy function and international patent application no WOOD/ 47561 discloses prodrugs of such compounds.
Although the urea and thiourea NNRTI disclosed in the above documents are active against reverse transcriptase, especially that of HIV-1, the nature of the HIV virus with its extreme lack of replicative fidelity and consequent tendency to rapid resistance development prompts a demand for further antiretroviral agents with enhanced antiviral performance against problematic drug escape mutants, notably at the RT 100, 103 and/or 181 positions.

Additionally, modern HIV therapy regimes, denoted HAART, Highly Active Anti Retroviral Therapy, administer antivirals as combinations of three or more antivirals of various classes, which combinations are administered for prolonged periods, if not for life. HAART requires the patient to follow a complicated dosing schedule with sometimes dozens of tablets per day taken at various times of the day in some cases before and in other cases after the ingestion of food.-There is thus a need for antiretroviral preparations allowing greater flexibility in dosing to facilitate patient compliance.
Brief description of the invention
In accordance with a first aspect of the invention there are provided
compounds of the (Formula Remove)


H
R6

RI

R3
where;
R! isO, S;
R2 is an optionally substituted, nitrogen-containing heterocycle, wherein the
nitrogen is located at the 2 position relative to the (thio)urea bond;
R3 is H, d-C3 alky!,
R4-Ry are independently selected from H, d-Ce alky!, Cg-Ce alkenyl, C2-Cs
alkynyl, haloCrC6 alkyl, CrG6 alkanoyl, haloCrC6 alkanoyl, CrC6 alkoxy,
halod-Ce alkoxy, Ci-C6 aIkyloxy-Ci-C6 alkyl, halod-Ce alkyloxy-CrC6 alkyl
hydroxy-d-Ce alkyl, amino-d-Ce alkyl, carboxy-Ct-Ce alkyl, cyano-d-Ce
alkyl, amino, carboxy, carbamoyl, cyano, halo, hydroxy, keto and the like;
X is -(CH2)n-D-(CH2)m-
D is -NR8-, -0-, -S-, -S(=O)- or-S(=O)2-
R8 is H, d-Ca alkyl
n and m are independently 0 or 1;
and pharmaceutically acceptable salts and prodrugs thereof.

The currently preferred value for R-\ is 0, that is a urea derivative, although RI as S (ie a thiourea derivative) is also highly potent.
Representative values for Ra include thiazolyl, pyridyl, pyrimidyi, pyrazinyl, pyridazinyl, pyrrolyl, imidazolyl, indolyl, triazolyl, tetrazolyl, piperidyl, piperazinyl and fused rings such as benzothiazolyl, benzopyridyl, benzodiazolyl, benzimidazolyl, quinolyl, purinyl and the like, any of which can be optionally substituted.
Preferred R2 values include pyrid-2-yl and thiazol-2-yl.
The optional substituents to R2 can include up to three substituents such as Ci-C6 alkyl, CrC6 alkoxy, C2-C6 alkenyl, G2-C8 alkynyl, C2.C8 alkenoxy, CrC6 alkoxy C-i-Ce alkyl, d-Ce alkanoyl, halod-Ce alkyl, Ci-C4 alkanoyloxy, d-C4 alkylthio, amino (including CrCa alkyl-substituted amino), carboxy, carbamoyi, cyano, halo, hydroxy, aminomethyl, carboxymethyl, hydroxymethyl, nitro, aryl, (such as phenyl, pyrrol-1-yl, tetrazol-5-yl, triazol-4-yl, pyridyl, pyrimidyi, pyrazinyl, imidazolyl, indolyl, piperidyl, piperazinyl, and the like) substituted (as herein defined) aryi, or -S02Q or-C(=O)Q, where Q is CrC6 alky!, halosubstituted C-i-Cs alkyl, aryl (as herein defined), substituted (as herein defined) aryl or amino. Heteroatoms in R2 can be derivatised, such as with Ct-C6 alkyl, oxo and the like. The optional Ra substituent may be ortho or meta to the bond to the (thio)urea function but is preferably para.
Preferred optional substituents to R2 include ethynyl, phenoxy, pyrrid-1-yl, cyclopropyl, phenyl, halo-substituted phenyl (especially para and meta chloro and fluorophenyl), and dimethylamino. Particularly preferred R2 substituents include halo (F, Br, Cl and I) and cyano. Preferred halo groups include Cl.
The currently preferred value for R3 is H.
Preferably R4 is hydrogen, halo or hydroxy, especially fluoro.

Preferably R5 is halo, Ci_3 alkylcarbonyl, C1-3alkyloxy or H, especially fluoro and most preferably H.
Preferably R6 is hydrogen, halo, CrC3alkyloxy, C1-3alkylcarbonyl, cyano or ethynyl, especially methoxy or fluoro and most preferably H.
Preferably R7 is hydrogen, halo, Gt-salkyloxy, or d-aalkylcarbonyl, most preferably fluoro.
Preferably R5 and Re are H and R4 and R7are halo, most preferably both are fluoro.
Preferably D is -O-, n is 0, m is 1, Ri is O, R2 is substituted pyrid-2-yl and R3 is H. An alternative preferred embodiment embraces compounds wherein D is -O-, n is 0, m is 1, RI is S, R2 is substituted pyrid-2-yl and R3 is H.
The compounds of formula I may be administered as a racemic mixture, but preferably the cyclopropyl moiety intermediate the (thio)urea function, X and the phenyl ring (denoted Y below) is at least 75% such as around 90% enantiomerically pure with respect to the conformation:

(Formula Remove)
Prefered optical isomers of the compounds of formula I show a negative optical rotation value. Such isomers, for example when X is -O-CHa-, tend to elute less rapidly from a chiral chromatagrarn, for example chiral AGP 150 x 10 mm, 5|j.m; Crom Tech LTD Colomn, flow rate 4 ml/min, mobile phase 89 vol % 10mM HOAc/NH4OAc in acetonitrile. On the basis of preliminary x-ray crystallography analysis a presently favoured absolute configuration appears to be:


H

The currently preferred value for D is -O-. Convenient values for n and m include 1:0 and 1:1. Preferred values of n:m include 0:2 and especially 0:1, that is a chroman derivative. Particularly preferred compounds have stereochemistry corresponding to (1S, 1 aflybffl-l .1 a.2,7b-tetrahvdrocvclopropafclchromen-l-vl. For the sake of clarity, it is noted that the structure:


(Formula Remove)

is equal to
R5


The expression CrCn alkyl, where n is 3, 6, 7 etc or lower alkyl includes such groups as methyl, ethyl, n-propyl, isopropyl, n-butyl, s-butyl, t-butyl, n-pentyl, n-hexyl, 3-methyl pentyl and the like.The term halo refers to chloro, bromo, fluoro and iodo, especially fluoro. CrCn alkoxy refers to groups such as methoxy, ethoxy, propoxy, t-butoxy and the like. C2-Cn alkenyl,refers to groups such as vinyl, 1-propen-2-yl, 1-buten-4-yl, l-penten-5-yl, 1-buten-1-yl and the like. CrCn alkylthio includes methylthio, ethylthio, t-butylthio and the like. CrCn alkanoyloxy includes acetoxy, propionoxy, formyloxy, butyryloxy and the like. C2-Cn alkenoxy includes ethenyloxy, propenyloxy, iso-butoxyethenyl and the like. HaloC-i-Cn alkyl (including complex substituents
comprising this moiety such as haloCi-Cn alkyloxy) includes alkyls as defined herein substituted 1 to 3 times by a halogen including trifluormethyl, 2-dichloroethyl, 3,3-difluoropropyl and the like. The term amine includes goups such as NH2, NHMe, N(Me)2 which may optionally be substituted with halogen, d-C/ acyloxy, CrC6 alkyl, CrC6 alkoxy, nitro, carboxy, carbamoyl, carbamoyloxy cyano, methylsulphonylamino and the like. Carboxy, carboxymethyl and carbamoyl include the corresponding pharmaceutically acceptable CrC6 alkyl and aryl esters.
Prodrugs of the compounds of formula I are those compounds which following administration to a patient release a compound of the formula I in vivo. Typical prodrugs are pharmaceutically acceptable ethers and especially esters (including phosphate esters) when any of R^Rr or the optional substituent to R2 represent an hydroxy function, pharmaceutically acceptable amides or carbamates when any of the Ra substituent or R4-R7 represent an amine function or pharmaceutically acceptable esters when the Ra substituent or R4-R7 represent a carboxy function.
The compounds' of formula I can form salts which form an additional aspect of the invention. Appropriate pharmaceutically acceptable salts of the compounds of formula I include salts of organic acids, especially carboxylic acids, including but not limited to acetate, trifluoroacetate, lactate, gluconate, citrate, tartrate, maleate, malate, pantothenate, isethionate, adipate, alginate, aspartate, benzoate, butyrate, digluconate, cyclopentanate, glucoheptanate, glycerophosphate, oxalate, heptanoate, hexanoate, fumarate, nicotinate, palmoate, pectinate, 3-phenylpropionate, picrate, pivalate, proprionate, tartrate, lactobionate, pivolate, camphorate, undecanoate and succinate, organic sulphonic acids such as methanesulphonate, ethanesulphonate, 2-hydroxyethane sulphonate, camphorsulphonate, 2-napthalenesulphonate, benzenesulphonate, p-chlorobenzenesulphonate and p-toluenesulphonate; and inorganic acids such as hydrochloride, hydrobromide, hydroiodide, sulphate, bisulphate, hemisulphate, thiocyanate, persulphate, phosphoric and sulphonic acids.
Hydroxy protecting group as used herein refers to a substituent which protects hydroxy! groups against undesirable reactions during synthetic procedures such as those O-protecting groups disclosed in Greene, "Protective Groups In Organic Synthesis," (John Wiley & Sons, New York (1981)). Hydroxy protecting groups comprise substituted methyl ethers, for example, methoxymethyl, benzyloxymethyl,-2-methoxyethoxymethyl, 2-(trimethylsilyl)ethoxymethyl, t-butyl and other lower alkyl ethers, such as isopropyl, ethyl and especially methyl, benzyl and triphenylmethyl; tetrahydropyranyl ethers; substituted ethyl ethers, for example, 2,2,2-trichioroethyl; silyl ethers, for example, trimethylsilyl, t-butyldimethylsilyl and t-butyldiphenylsilyl; and esters prepared by reacting the hydroxyl group with a carboxylic acid, for example, acetate, propionate, benzoate and the like.
The invention further provides pharmaceutical compositions comprising the compounds of the invention and pharmaceutically acceptable carriers or diluents therefor. Additional aspects of the invention provide methods for the inhibition of HIV comprising administering a compound of the formula I to a subject afflicted with or exposed to HIV-1. The HIV-1 may comprise a drug escape mutant, such as HIV strain comprising the mutations at the 100, 103 and/or 181 mutations, especially K103N.
The invention also extends to the use of the compounds of formula I in therapy, such as in the preparation of a medicament for the treatment of HIV infections.
In treating conditions caused by HIV, the compounds of formula I are preferably administered in an amount to achieve a plasma level of around 100 to 5000 nM, such as 300 to 2000 nM. This corresponds to a dosage rate, depending on the bioavailability of the formulation, of the order 0.01 to 10 mg/kg/day, preferably 0.1 to 2 mg/kg/day. A typical dosage rate for a normal adult will be around 0.05 to 5 g per day, preferably 0.1 to 2 g such as 500-750 mg, in one to four dosage units per day. As with all Pharmaceuticals, dosage rates will vary with the size and metabolic condition of the patient as well as
the severity of the infection and may need to be adjusted for concomitant medications.
In keeping with the usual practice with HIV inhibitors it is advantageous to co-administer one to three additional antivirals to provide synergistic responses and to ensure complementary resistance patterns. Such additional antivirals may include AZT-; ddl, ddC, D4T, 3TC.-DAPD, alovudine, abacavirr adefovir, adefovir dipivoxil, bis-POC-PMPA, GW420 867X, foscarnet, hydroxyurea, Hoechst-Bayer HBY 097, efavirenz, trovirdine, capravirine, nevirapine, delaviridine, tipranavir, emtricitabine, PFA, H2G (omaciclovir), MIV-606 (valomaciclovir stearate), TMC-126, TMC-125, TMC-120, efavirenz, DMP-450, loviride, ritonavir, (including kaletra), lopinavir, saquinavir, lasinavir, indinavir, amprenavir, amprenavir phosphate, nelfinavir and the like, typically at molar ratios reflecting their respective activities and bioavailabilities. Generally such ratio will be of the order of 25:1 to 1:25, relative to the compound of formula I, but may be lower, for instance in the case of cytochrome antagonists such as ritonavir.
Compounds of the invention are typically prepared as follows:
Scheme 1

R10
(Formula Remove)

M.DPPA, Et3N, toluene; (b) substituted 2-aminopyridine; (c) aqueous HCI, dioxane; (d) substituted 2-pyridyl isothiocyanate.

Compounds of the general formula (I), wherein RI is O (urea) or S (thiourea), R2 is, for instance, a 5-substituted pyrid-2-yl, and Ra is H, are prepared by methods shown in Scheme 1. The cyclopropanecarboxylie acid 1-Scheme-1 is converted to the acyl azide and heated to 120 °C to induce Curtius rearrangement and provide the isocyanate 2-Scheme-1. The urea 3-Scheme-
1 is obtained by coupling of the isocyanate with the relevantly substituted 2-
• aminopyridine. Hydrolysis of the isocyanate'as instep (c)-which results in the • cyclopropylamine 4-Scheme-1, followed by reaction with a 2-pyridyl isothiocyanate provides the thiourea 5-Scheme-1. The isothiocyanate may be prepared from the optionally ring substituted 2-aminopyridine by known methods, such as treatment with thiophosgene orthiocarbonyldiimidazole. R3 variants of formula I are prepared correspondingly using the appropriately amine-substituted amino-Ra, ie 2-(N-methylamino)pyridine for Ra as methyl. Many 2-aminopyridines are commercially available and others are described in literature, for example those shown in Scheme 2. Ri=S compounds can alternatively be prepared from the isothiocyanate corresponding to 2-Scheme
2 or from amine 3-Scheme 2 and amino-Ra in conjunction with an RC(=S)R'
both as described in WO 9303022. Although Scheme 1 has been illustrated
with a substituted pyridyl it is readily apparent that corresponding couplings
can be used for other R2 variants such as optionally substituted thiazolyl,
pyrazinyl, benzothiazolyl, pyrimidinyl etc.


Scheme 2
Br



(Formula Remove)


c
]•.
H2N N
(a) phenol, NaH, DMF; (b) 10% Pd/C, H2 1 atm, EtOH; (c) PdCI2(PPh3)2l trimethylsilylacetylene.Cul, diisopropylamine; (d) tert-butylammonium fluoride
Replacement of the bromine in 5-bromo-2-nitropyridine by a phenoxy group, followed by reduction of the nitro group affords the 2-amino-5-phenoxypyridine. The Sonogashira coupling of 2-amino-5-iodopyridine with the terminal alkyne SiMe3C=CH in the presence of catalytic amounts of bis(triphenylphosphine)palladium dichloride and cuprous iodide as in step (c) provides the 2-amino-5-(2-trimethylsilyiethynyl)pyridine. Removal of the silyl group by TBAF yields 2-amino-5-ethynylpyridine which can be coupled to the isocyanate as described in Scheme 1. Alternatively, treatment with TBAF may be performed on the urea 3-Scheme-1 or thiourea 5-Scheme-1 where R10 is -C=CSiMe3 to convert R10 to -C=CH.
bcneme y



COOEt
(Formula Remove)
COOEt


R10
(a) ethyl diazoacetate, catalyst, CH2CI2; (b) chromatography and then reflux with LiOH, H2O, MeOH; (c) reflux with LiOH, H2O, MeOH and then chromatography; (d) rt, NaOH, H2O, MeOH and then reflux with LiOH, H2O, MeOH
Compounds of the general formula (I), wherein R1 is O (urea) or S (thiourea), R2 is, for example, a 5-substituted pyrid-2-yl, R3 is H, X is -D-CH2, and wherein the cyclopropyl moiety has the relative configuration
X
are prepared by methods shown in Scheme 3. Cyclopropanation of the double bond in the chromene 1-Scheme-3 with ethyl diazoacetate is catalyzed by cuprous or rhodiurn(ll) salts such as Cul, (CuOTf)2-benzene, and Rh2(OAc)4 in solvents such as dichloromethane, 1,2-dichloroethane, or chloroform. The reaction provides a diastereomeric mixture of the cyclopropanecarboxylic acid ethyl esters 2-Scheme-3. with the all cis relative configuration, and its trans isomer 3-Scheme-3. Separation by column chromatography of the cis and trans diastereomers may be accomplished at this stage, followed by
hydrolysis of the isolated 2-Scheme-3, such as by retluxlng in aqueous methanolic LiOH, to yield a racemic mixture of the all cis cyclopropanecarboxylic acid 4-Scheme-3. as described in step (b). Alternatively, the diastereomeric mixture of ethyl esters may be subjected to hydrolysis, and separation conducted on the mixture of cyclopropanecarboxylic acids to provide the isolated all cis isomer, as in step •-(c). Step' (d) involves isolation of the cis ethyl 'ester 2-Scheme-3 which may also be done by selective hydrolysis of the trans 3-Scheme-3 at lower temperatures, such as treatment with aqueous methanolic NaOH at ambient temperature. The isolated cis ethyl ester may then be hydrolyzed in the usual manner to the cyclopropanecarboxylic acid 4-Scheme-3. The cyclopropanecarboxyiic acid is subjected to the methods outlined in Scheme 1 to obtain the urea or thiourea 5-Scheme-3. The chromenes 1 -Scheme-3 are prepared by methods shown in Schemes 4, 5, and 6. Although this scheme 3 has been illustrated with a D=O variant it will be apparent that corresponding manipulations will be available to the D=S, S=O; S(=O)2 and D=NR8 variants. When R8 is H, the nitrogen is typically protected with a conventional secondary amine protecting group, such as those described in 'Greene & Wuts Protective Groups in Organic Synthesis 2nd ed, Wiley NY 1991).
Scheme 4
(Formula Remove)


R5'
R4
1 (a) 3-bromopropyne, K2C03, acetone; (b) N,N-diethylaniline or PEG-200, 225
Scheme 4 describes the preparation of chromenes, including many from commercially available disubstituted phenols, such as those wherein the substitution pattern in the benzene ring is as follows: R4 and R7 are halo; R4 and R6 are halo; R5 and R7 are halo; R4 is halo and R7 is Ci-3 alkylcarbonyl; and R4 is hydroxy while R5 is Ci.3 alkylcarbonyl. Reaction of the available
disubstituted phenol 1-Scheme-4 with 3-bromopropyne in the presence of a base, such as K2CO3 in acetone or NaH in DMF, results in nucleophilic substitution of the halide to provide the ether 2-Scheme-4. Ring closure may be accomplished by heating the ether in N, N-dimethylaniline or polyethylene giycol to yield the chromene 3-Scheme-4.
Scheme 5
(Formula Remove)

NaBH4, EtOH; (b) p-toiuenesulfonic acid, toluene, reflux;
Scheme 5 describes the preparation of chromenes, used as starting material
in Scheme 3, from the appropriately substituted chromanones, which are
readily accessed from commercially available chromanones, for example
those wherein one of the positions in R4 to R7 is substituted with halo or Ci-a
alkoxy. Conversion of the carbonyl group in 4-chromanone 1 a-Scherne-5 and
to the correponding alcohol by a suitable reducing agent such sodium
borohydride in ethanol provides 2-Scheme-5. Refiuxing the alcohol with small
amounts of acid, such as p-TsOH in toluene, causes dehydration of 2;
Scheme-5 to the desired chromene 1 -Scheme-3. Corresponding
manipulations will be available for other D variants. For example the
corresponding 2H-1-benzothiopyran is readily prepared from commercially
available (substituted) thiochroman-4-ones by reaction- with a reductant such
as a metal hydride for example lithium aluminium hydride in an organic
solvent such as ether, followed by dehydration such as refluxing with an acid
for example potassium acid sulphate or the like.

Scheme 6
(Formula Remove)



(a) allyl bromide, K2CO3, acetone; (b) Ph3PCH3Br, NaH, THF; (c) CI2[Pcy3]2Ru=CHPh, CH2CI2 (d) Ph3P+CH=CH2 Br', DBU

Chromenes, for use as starting material in Scheme 3, are prepared from substituted o-hydroxybenzaldehydes as shown by methods outlined in Scheme 6. Reaction of 1 -Scheme-6 with allyl bromide in the presence of a base, such as K2CO3 in acetone, results in nucleophilic substitution of the halide to provide the ether 2-Scheme-6. Witting reaction transforms the aldehydic group into the olefin and provides 3-Scheme-6. The pair of terminal double bonds may undergo metathesis intramolecularly by treatment with a catalyst such as the ruthenium complex Grubb's catalyst in step (c) to produce the chromene. Alternatively 1-Scheme-6 can be cyclised directly as shown in step d) in the legend above.
Scheme 7

TfO
COEt

COOEt

Me3Si

COOEt
COOEt

.,., 3

COOH

(Formula Remove)
(a) Pd(0), DPPP, Et3N, (CH3)3SiC=CH; (b) Pd(0), butyl vinyl ether, DMF; (c) Pd(0), Zn(CN)2) DMF; (d) NaOH, H2O, MeOH
Pd(0) catalyzed coupling of the triflate 1-Scheme-7 leads to the replacement of the trifluoromethanesulfonyloxy group and the introduction of other substiutents at R6. Thus, Scheme 7 provides the preparation of synthesis intermediates for use in scheme 3 to give the urea or thiourea 5-Scheme-3 wherein R6 is cyano, ethynyl, orCi.3alkylcarbonyl.
Scheme 8
(Formula Remove)
7 PG
(a) BuLi/ZnC!2, THF; Pd(OAc)2> BrCH=CHCOOEt; DIBAL
(b) TsNHN=CHCOCI; PhNMe2, NEt3, CH2CI2
(c) Rh2(5-R-MEPY)4, abs degassed dichloromethane
(d) 30% HBr, AcOH
(e) NaOH, H2O
(f) NaOH; CO2; l-Prl/DMSO
(g) IPrOH, HCI; DEAD, PPh3) THF
(h) NaOH, MeOH:H20
(i) 1. BBr3, CH2CI2 2. CH3CN 3. NaOH, water
(j) 1. BuLi/ZnCl2, THF; Pd(OAc) 2. cpd 9-.Scheme-8 3. Jones reagent
(chromic acid, suifuric acid in acetone)
Convenient routes to compounds wherein X is -CHa-O- are depicted in Scheme 8, where Ra and Rb are optional substituents FU-R/, which are suitably protected with conventional protecting groups as necessary and R° is a lower alkyl ester. Optionally substituted phenol 1 -Scheme-8 which is hydroxy-protected with a protecting group such as methyl, MOM and the like is reacted with a base such as BuLi or the like in a solvent such as THF or the like-a'nd transformed to zinc salt by adding zinc chloride or the like. A' " ' catalyst such as Pd(OAc)2 or the like is added along with an activated acrylate such as lower alkyl-cis-3-haloacrylate, for example BrCH=CHCOOEt or the like. The reaction mixture is cooled and a reducing agent such as DIBAL or the like is added portionwise and quenched to yield 2-Scheme-8. A hydrazone such as the p-toluenesulfonylhydrazone of glyoxylic acid chloride or the like and a base such as N,N-dimethylaniline or the like is added in a solvent such as CH2CI2 or the like followed by the addition of another base such as Et3N or the like to yield 3-Scheme-8. The reaction product is dissolved in a solvent such as dichloromethane or the like which is preferably degassed. A chira! Doyle's catalyst such as Rhi2(5-R-IVIEPy)4 (US 5175311, available from Aidrich or Johnson Matthey), or the like is added to yield 4^ Scheme-8 in a high enantiomeric excess such as greater than 80, preferably greater than 90% ee. Preferably, this compound is first reacted with BBra in dichloromethane followed by the addition of acetonitrile the reaction mixture and finally sodiumhydroxjde is added to give 6-Scheme-8. Alternatively, this .product (4-Scheme-8) is ring-opened with an electrophile preferably HBr or the like under in conjunction with an acid such as AcOH or the like. Under acid conditions a spontaneous ring closure takes place to form chromenone 5^ Scheme-8. When subjected to basic conditions such as NaOH or the like, the chromenone rearranges to form the chromencyclopropylcarboxylic acid 61 Scheme-8. Alternatively, 4-Scheme-8, for instance when the phenolic protecting group is MOM, can be subjected to basic conditions such as NaOH, carbon dioxide and a lower alkyl halide such as iPrl in a solvent such as DMSO to open the iactone and yield the alkyl ester 7-Scheme-8. Displacement of the hydroxy protecting group and ring closure with the free hydroxymethyl moiety occurs in acidic conditions such as iPrOH/HCI or the like followed by DEAD; PPHs in an organic solvent such as THF or the like.
Alternatively, in a convergent approach, compound 1 -Scheme-8 is reacted with BuLi and transformed to a zinc salt. This salt reacted with the cyclopropyliodide, 9-Scheme-8. in a palladium-catalyzed reaction to give after reaction with Jone's reagent compound 4-Scheme-8. This carboxylic acid is in turn converted to the isocyanate as shown in Scheme 1 and subsequently to the heteroarylurea or heteroarylthiourea of the Formula I.
A further aspect of the invention provides novel intermediates useful in the above described syntheses of the compound of formula I. A preferred group of intermediates include compounds of the formula II:
(Formula Remove)

R4
where X and R-rRy are as defined above and RH is -C(O)ORi2, where Rjs is H or a carboxy protecting group such as a lower alkyl ester; -NCO, -NCS or an amine such as Nhk. A favoured subset of the compounds of formula II have the formula III:
R11
(Formula Remove)

where R4 and Ry are independently halo, most preferably fluoro, and Rn is -COOH, a lower alkyl ester thereof, isocyanate, isothiocyanate or amino.
A further group of preferred intermediates includes compounds of the formul, IV
(Formula Remove)

R7 IV .
where R4 to R7 are as defined above, PG is an hydroxy protecting group and PG* is an hydroxy protecting group or together with the adjacent O defines a keto function.
A preferred subset of compounds of formula IV are those of formula V:

PG*—O
(Formula Remove)
O-PG
R7 'V

where R4 and R7are independently halo, most preferably fluoro, PG is lower alkyl, such as isopropyl, ethyl and most preferably methyl and PG* is lower alkyl such as isopropyl, ethyl and most preferably methyl or together with the adjacent O defines a keto group

A still further group of preferred intermediates includes compounds of the formula VI:
R4

where FlrRy are as defined above, PG is an hydroxy protecting group and Ria is H, an ester thereof or an hydroxy protecting group. A preferred subset within formula VI has the formula VII:
(Formula Remove)
R4
,R13
R7 VII.
where R4 and R7 are independently halo, preferably fluoro, PG is lower alkyl, such as isopropyl, ethyl and most preferably methyl and R^ is H or -C(=O)CH=N=N.
Favoured compounds of formula I include
c/s-1 -(5-Cyano-pyridin-2-yl)-3-(1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -
yl)-urea,
c/s-1 -(5-Cyano-pyridin-2-yl)-3-(1,1 a,3,7b-tetrahydro-2-oxa-
cyclopropa[a]naphtha!en-1-yl)-urea,
c/s-1 -(5-Cyano-pyridin-2-yl)-3-(7-hydroxy-6-propionyl-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea,
c/s-1 -(6-Acety!-7-hydroxy-1,1 a^^b-tetrahydro-cyclopropafcjchromen-'l -yl)-3-
(5-cyano-pyridin-2-yl)-urea,
c/s-1-(5-Cyanopyridin-2-yl)-3-(7-fluoro-4-propionyl-1l1a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea, .'..... ..._„.. ..........
c/s-1 -(5-Cyano-pyridin-2-y!)-3-(7-fluoro-4-methoxy-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea,
c/s-1-(S-Cyano-pyridin^-yO-S^-fluoro^-chloro-l.la^yb-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea,
c/s-1 -(5-Chloro-pyridin-2-yl)-3-(4-chloro-7-fluoro-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromene-1-yl)-urea,
c/s-1 -(5-Bromo-pyridin-2-yl)-3-(4-chloro-7-fluoro-1,1a,2,7b-tetrahydro-
cyclopropa[c]chromene-1-yl)-urea,
c/s-1 -(5-Cyano-pyridin-2-yl)-3-(5-cyano-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea,

c/s-1 -(5-Cyano-pyridin-2-yl)-3-(5-ethynyl-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea,
c/s-1 -(5-Acetyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)-3-(5-cyano-
pyridin-2-yl)-urea,
c/s-1-(5-Methoxy-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-cyano-
pyridin-2-yl)-urea,
" c/s-1-(5-Cyano-pyridin-2-yl)-3-(A/-acetyl-1,1 a,3,7b-tetrahydro-2-oxa-
cyclopropa[a]quinoline-1-yl))-urea,
c/s-1-(5-Cyano-3-methyl-pyridin-2-yl)-3-(4l7-difluoro-1,1a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea,
c/s-1 -(4,7-Difluoro-1, 1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)-3-(5-
ethynyI-pyridin-2-yl)-urea,
c/s-1-(5-Bromo-pyridin-2-yl)-3-(4,7-difIuoro-1,1a,2,7b-tetrahydro-
cyclopropa[c]chromen-1 -yl)-urea,
c/s-1 -(4,7-Dif luoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)-3-(5-
phenoxy-pyridin-2-yl)-urea,
c/s-1-(5-Cyano-pyridin-2-yI)-3-(4,7-dif!uoro-1,1a,2,7b-tetrahydro-
cyclopropa[c]chromen-1 -yl)-thiourea,
1-(6-Chloro-5-cyano-pyridin-2-yl)-3-(5,7-difluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)urea,
1-(5-Cyano-pyridin-2-yl)-3-(5,7-dif!uoro-1,1a,2)7b-tetrahydro-
cyclopropa[c]chromen-1-yl)urea,
c/s-1-(4-Bromo-7-f Iuoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-
cyano-pyridin-2-yl)-urea,
c/s-1 -(4-Bromo-7-f!uoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-y!)-3-(6-
chloro-5-cyano-pyridin-2-yl)-urea,
c/s-1 -(4-Bromo-6-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-l-yl)-3-(5-
cyano-pyridin-2-yl)-urea,
c/s-1-(4-Bromo-6-f!uoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(6-
cloro-5-cyano-pyridin-2-yl)-urea,
c/s-1 -(5-Cyanopyridin-2-yl)-3-(6-f luoro-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)urea,
c/s A/-[1 a, 6&-dihydro-1 H-benzo[b]cycIopropa[d]thien-1 -yl]-A/-(5-cyano-2-
pyridinyl)-urea,

A/-[(1S, 1aR, 7bR) or(1R, 1ab, /'bb)-l. la, 2, /b-tetrahydrocyclopropa[c]-
[1 ]benzothiopyran-1 -yl]-A/'-(5-cyano-2-pyridinyI) urea,
c/s-A/-(5-bromo-2-pyridinyl)-/V-(7-chloro-4-f!uoro-1,1a,2I7b-
tetrahydrocyclopropa[c]chromen-1-yl)urea,
c/s-/V-(7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromen-1-yl)-A/-(5-
chloro-2-pyridinyl)urea,
c/s-N-(7-chloro-4-fluoro-1j1a,2,7b-tetrahydroeyclopropa[c]chromen-1-yl)-yV-(5-"
cyano-2-pyridinyl)urea,
c/'s-A/-(5-phenoxy-2-pyridinyl)-A/-(4,7-dichloro-1,1 a,2,7b-
tetrahydrocyclopropa[c]chromen-1 -yl)urea,
c/s-/V-(5-bromo-2-pyridinyl)-W-(4,7-dichloro-1,1 a,2,7b-
tetrahydrocyclopropa[c]chromen-1 -yl)urea ,
c/s-/V-(5-chloro-2-pyridinyl)-/V-(4,7-dichloro-1,1 a,2,7b-
tetrahydrocyclopropa[c]chromen-1-yl)urea,
c/s-/V-(5-cyano-2-pyridinyl)-A/-(4,7-dichloro-1,1 a,2,7b-
tetrahydrocyclopropa[c]chromen-1 -yl)urea,
A/-[(1 S, 1 aRjbR^J-diiluoro-l ,1 a,2,7b-tetrahydrocyclopropa[c]chromen-1 -yl]-
/V-(5-fluoro-2-pyridinyl)urea,
/V-[(1S,1aH7bf?)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-1-yl]-
A/-(5-iodo-2-pyridinyl)urea,
A/-[(1 S, 1 afl,7bff)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-1 -yt]-
/V-(3-isoxazolyl)urea,
/V-[(.1S,1a.£?,7b/:?)t4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-lTyl]T, ..., . .....
/V-[4-(4-chIorophenyl)-1,3-thiazol-2-yl]urea,
A/-[(1 S,1 af?,7bff)-4,7-difluoro-1 .la^^b-tetrahydrocyclopropatcjchromen-l-yl]-
/V-(6-fluoro-1,3-benzothiazol-2-yl)urea,
/V-[(1 S,1 aR,7bR)-4J-d\nuom-~\ .la^Jb-tetrahydrocyclopropafclchromen-l-yl]-
/V-(4-pyrimidinyl)urea
A/-[(1 S,1 aH7bfl)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c|chromen-1-yl]-
A/-(2-pyrazinyl)urea,
A/-[(1 S,1 afljb^^^-difluoro-l ,1a,2,7b-tetrahydrocyclopropa[c]chromen-1-yl]-
/V-(5-cyclopropyl-1/-/-pyrazol-3-yl)urea

and pharmaceutically acceptable salts thereof, especially enantfomerically enriched, for example greater than 80% by weight, preferably > 90%, such as >97% ee or pure preparations comprising the (-) enantiomer.
Particularly preferred compound thus include
(-)-cis--l -(5-Cyano-pyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chrorn'en-1-yl)-urea, •--..• ........ .....,.-.• ... ,,
(-) c/s-1-(5-Chloro-pyridin-2-yl)-3-(4,7-difluoro-1,1al2,7b-tetrahydro-
cyclopropa[c]chromen-1 -yl)-urea; or
(-J-c/s-1 -(5-Cyano-pyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-thiourea;
('->c/s-1-(5-Fluoropyridin-2-yl)-3-(4,7-difluoro-1,1a,2,7b-tetrahydro-
cyc!opropa[c]chromen-1-y!)-urea,
(-)-cisA -(5-Fluoropyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-thiourea;
and pharmaceutically acceptable salts thereof.
While it is possible for the active agent to be administered alone, it is preferable to present it as part of a pharmaceutical formulation. Such a formulation will comprise the above defined active agent together with one or more acceptable carriers or excipients and optionally other therapeutic ingredients. The carrier(s) must be acceptable in the sense of being compatible with-the other ingredients of the formulation and-not deleterious to the recipient.
The formulations include those suitable for rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration, but preferably the formulation is an orally administered formulation. The formulations may conveniently be presented in unit dosage form, e.g. tablets and sustained release capsules, and may be prepared by any methods well known in the art of pharmacy.

Such methods include the step of bringing into association the above defined active agent with the carrier. In general, the formulations are prepared by uniformly and intimately bringing into association the active agent with liquid carriers or finely divided solid carriers or both, and then if necessary shaping the product. The invention extends to methods for preparing a pharmaceutical composition comprising bringing a compound of Formula I or its - pharmaceutically acceptable salt in conjunction-or association' with a pharmaceutically acceptable carrier or vehicle. If the manufacture of pharmaceutical formulations involves intimate mixing of pharmaceutical excipients and the active ingredient in salt form, then it is often preferred to use excipients which are non-basic in nature, i.e. either acidic or neutral. Formulations for oral administration in the present invention may be presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active agent; as a powder or granules; as a solution or a suspension of the active agent in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water in oil liquid emulsion and as a bolus etc.
With regard to compositions for oral administration (e.g. tablets and'Capsules), the term suitable carrier includes vehicles such as common excipients e.g. binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, polyvinylpyrrolidone (Povidone), methylcellulose, ethylcellulose, sodium carbpxymethylcellulose, hydroxypropylmethylcellulose^sucrose and starch; . fillers and carriers, for example corn starch, gelatin, lactose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride and alginic acid; and lubricants such as magnesium stearate, sodium stearate and other metallic stearates, stearic acid, glycerol stearate, silicone fluid, talc waxes, oils and colloidal silica. Flavouring agents such as peppermint, oil of wintergreen, cherry flavouring or the like can also be used. It may be desirable to add a colouring agent to make the dosage form readily identifiable. Tablets may also be coated by methods well known in the art. A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active agent in a free flowing form such

as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface-active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may be optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active agent.
Other formulations suitable for oral administration include lozenges comprising the active agent in a flavoured base, usually sucrose and acacia or tragacanth; pastilles comprising the active agent in an inert base such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active agent in a suitable liquid carrier.
Detailed Description
Various aspects of the invention will now be illustrated by way of example only
with reference to the following non-limiting examples.
Example 1
(±) c/s-1 -(5-Cvano-Dvridin-2-yl)-3-(1.1 a,2,7b-tetranvdro-
cvclQproparclchromene-1-vn-urea.
a) ±c/s-1,1a,2,7b-Tetrahydro-cvcloproparclchromene-1 -carboxvlic acid ethyl este
(Formula Remove)

To a mixture of 2/-/-chromene (4.89 g, 37 mmol) and (CuOTf)2-benzene (186 mg, 0.37 mmol) in 1,2-dichloroethane (80 mL) at 20 °C, was added dropwise (3h) a solution of ethyl diazoacetate (8.44 g, 74 mmol) in 1,2-dichloroethane (20 ml). After 15 min at 20 °C, the reaction mixture was washed with H2O (100 mL). The H2O phase was washed with CHaCIa (50 ml) and the solvent of the combined organic phases was removed under reduced pressure. The crude product was column chromatographed (silica gel, 20->50% EtOAc in

hexane), to give 1.96 g (24%) of ±cis-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester and 3.87 g (48%) of ±-trans-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester as a byproduct,
1H-NMR (CDCI3): 7.26 (d, 1H), 7.10 (dd, 1H), 6.90 (dd, 1H), 6.78 (d, 1H), 4.49 (dd; TH);4.20 (dd, 1H)',3:97 (q, 2H), 2.44 (dd, 1H), 2.14 (titi,1H), 2.07-1.95"' (m, 1H), 1.02 (t, 3H).
b) (±)-c/s-1,1 a,2,7b-Tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
(Formula Remove)

A mixture of (±)-c/s-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic
acid ethyl ester (1.96 g, 9.0 mmol), LiOH (539 mg, 22.5 mmol), H2O (10 mL).
and MeOH (20 mL) was heated to reflux for 2h. The reaction mixture was
concentrated to about 10 mL, 4N HCI was added dropwise giving a white
precipitate. The reaction mixture was extracted with CHaCIa (3x15 mL) and the
solvent of the combined organic phases was removed under reduced
pressure. The crude product was crystallized from EtOAc/hexane, to give 435
mg (25%) of (±)-c/s-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic
acid as a white solid. ,.•.,...
1H-NMR (CDCIa): 9.80 (brs, 1H), 7.22 (d, 1H), 7.10 (dd, 1H), 6.89 (dd, 1H), 6.77 (d, 1H), 4.45 (dd, 1H), 4.22 (dd, 1H), 2.45 (dd, 1H), 2.14-1.98 (m, 2H).
c) (±)-c/s-1 -(5-Cyano-pyridin-2-yi)-3-(1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-yl)-urea.
(Formula Remove)


To a solution of (±)-c/s-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (285 mg, 1.5 mmol) and triethylamine (209 u.L, 1.5 mmol) in toluene (1.5 ml) at 20 °C, was added diphenylphosphoryl azide (413 mg, 1.5 mrnol). After 30 min at 20 °C, the reaction mixture was heated to 120 °C for 15 min, where after a solution of 2-amino-5-cyano-pyridine (197 mg, 1.65 mmol) in DMF (1m!_) was added. After 3h at 120 °C, the reaction mixture was allowed to assume room temperature. The reaction mixture was concentrated under reduced pressure, benzene (20 ml) was added and the reaction mixture was washed with 1N HCI (30 ml), H2O (30 ml) and brine (30 mL). The solvent of the organic phases was removed under reduced pressure. The crude product was crystallized from EtOH/CH2CI2, to give 133 rng (29%) of (±) -c/s-1 -(5-cyano-pyridin-2-yl)-3-(1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-yl)-urea.
1H-NMR (DMSO-de): 9.78 (s, 1H), 8.31 (d, 1H), 7.99 (dd, 1H), 7.83 (d, 1H), 7.43 (d, 1H), 7.27 (d, 1H), 7.09 (dd, 1H), 6.89 (dd, 1H), 6.80 (d, 1H), 4.25 (dd, 1H),4.14(dd, 1H), 3.43 (m, 1H), 2.35 (dd, 1H), 1.92 (m, 1H).
Example 2
(±)-c/s-1 -(5-Cyano-pvridin-2-vl)-3-f 1,1 a,3.7b-tetrahvdro-2-oxa-
cvclopropafa1naphthalen-1-yl)-urea.
a) (±)-c/s-1,1 a,3,7b-Tetrahydro-2-oxa-cyclopr6pa[a]riaphthalene-1 -carboxylic acid ethyl ester
(Formula Remove)

(±)-c/s-1,1a,3,7b-Tetrahydro-2-oxa-cyclopropa[a]naphthalene-1-carboxylic acid ethyl ester was synthesized analogously to Example 1 a from 1H-isochromene (3.57 g, 27 mmol), to give 910 mg (15%) of (±)-c/s-1,1a,3,7b-tetrahydro-2-oxa-cyclopropa[a]naphthalene-1 -carboxylic acid ethyl ester.

1H-NMR (CDCIa): 7.34 (d, 1H), 7.25 (dd, 1H), 7.18 (dd, 1H), 7.03 (d, 1H), 4.81 (d, 1 H), 4.51 (d, 1 H), 4.28 (dd, 1 H), 3.95 (q, 2H), 2.43 (dd, 1 H), 2.05 (dd, 1 H), 1 .04 (t, 3H).
b) (±)-c/s-1 ,1 a,3,7b-Tetrahydro-2-oxa-cyclopropa[a]naphthalene-1-carboxylic acid
(Formula Remove)

(±)-c/s-1 , 1 a,3,7b-Tetrahydro-2-oxa-cyclopropa[a]naphthalene-1 -carboxylic acid was synthesized analogously to Example 1b from (±)-c/s-1,1a,3,7b-tetrahydro-2-oxa-cyclopropa[a]naphthalene-1 -carboxylic acid ethyl ester (436 mg, 2 mmol), to give 86 mg (22%) of (±)-c/s-1,1a,3,7b-tetrahydro-2-oxa-cyclopropa[a]-naphthaiene-1 -carboxylic acid as a. white solid. The crude product was column chromatographed (silica gel, 1->5% MeOH in CH2CI2).
1H-NMR (CDCI3): 8.50 (brs, 1H), 7.39 (d, 1H), 7.30 (dd, 1H), 7.21 (dd, 1H), 7.07 (d, 1H), 4.87 (d, 1H), 4.57 (d, 1H), 4.38 (dd, 1H), 2.59 (dd, 1H), 2.15 (dd, IN)!
c) (±)-c/s-1 -(5-Cyano-pyridin-2-yl)-3-(1 , 1 a,3,7b-tetrahydro-2-oxa-cyc!opropa[a]naphthalen-1-yl)-urea
(Formula Remove)

(±)-c/s-1-(5-Cyano-pyridin-2-yl)-3-(1,1a,3,7b-tetrahydro-2-oxa-cyclopropa[a]naphthalen-1-yl)-urea was synthesized analogously to example 1 c from (±)-c/s-1,1 a,3,7b-tetrahydro-2-oxa-cyclopropa[a]naphthalene-1-carboxylic acid (86 mg, 0.45 mmol). The crude product was column chromatographed (silica gel, 1-»5% MeOH in CH2CI2), to give 21 mg (15%) of (±)-c/s-1 -(5-cyano-pyridin-2-yl)-3-(1,1 a,3,7b-tetrahydro-2-oxa-cyclopropa[a]naphthalen-1-yl)-urea.

1H-NMR (DMSO-d6): 9.62 (s, 1H), 8.29 (d, 1H), 7.98 (dd, 1H), 7.52-7.44 (m, 2H), 7.27-7.05 (m, 4H), 4.69 (d, 1H), 4.45 (d, 1H), 4.05 (dd, 1H), 3.25-3.10 (m, 1H),2.22(dd,
Example 3_
(±)-c/s-1 -(5-Cvano-pvridin-2-vl)-3-(7-hvdroxv-6-propionvl-1 ,1 a.2.7b-tetrahvdro-
cycloproparclchro'rrien-1 -yl)-urea. ' ' ...... "
a) 1 -(2-Hydroxy-4-prop-2-ynyloxy-phenyl)-propan-1 -one
HO
A mixture of 2',4'-dihydroxy-propiophenone (24.9 g, 0.15 mol), 3-bromo-propyne (24.2 g, 0.20 mol) and K2CO3 (20.7 g, 0.15 mol) in acetone (500 mL) was refluxed for 12 h. The reaction mixture was allowed assume room temperature and the precipitate was removed by filtration. The filtrate was concentrated under reduced pressure. The crude product was purified by column chromatography (silica gel, 0-»2% MeOH in HaO), to give 26.2 g '85%) of 1-(2-hydroxy-4-prop-2-ynyloxy-phenyl)-propan-1-one.
H-NMR (CDCIa): 12.80 (s, 1H), 7.69 (d, 1H), 6.52 (m, 2H), 4.72 (d, 2H), 2.96 (q,2H), 2.56 (t, 1H), 1.23 (t, 3H).
3b) 1 -(5-Hydroxy-2H-chromen-6-yl)-propan-1 -one.
(Formula Remove)

A mixture of 1-(2-hydroxy-4-prop-2-ynyloxy-phenyl)-propan-1-one (19.8 g, 97 mmol) and W,/V-diethy!aniline (100 mL) was heated to reflux for 3 h. The reaction mixture was concentrated under reduced pressure. The crude product was purified by column chromatography (silica gel, 5->10% EtOAc in Hexane) and thereafter recrystallized from EtOAc/Hexane, to give 8.91 g (45%) of 1-(5-hydroxy-2H-chromen-6-yl)-propan-1-one.

1H-NMR (CDCI3): 13.00 (s, 1H), 7.49 (d, 1H), 6.75 (dt, 1H), 6.27 (d, 1H), 5.67 (dt, 1H), 4.86 (dd, 2H), 2.90 (q, 2H), 1.19 (t, 3H).
3c) 7-Hydroxy-6-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -•carboxylic acid ethyl ester.
(Formula Remove)

Q"
To a mixture of 1-(5-hydroxy-2H-chrornen-6-yl)-propan-1-one (511 mg, 2.5 mmol) and (Rh(II)Ac2)2 (11 mg, 0.025 mmol) in 1,2-dichloroethane (8 ml) at 20 °C, was added dropwise (3h) a solution of ethyl diazoacetate (571 mg, 5 mmol) in 1,2-dichloroethane (2 mL). After 15 min at 20 °C, the reaction mixture was washed with H2O (10 ml). The H2O phase was washed with CHaCI2 (10 mL) and the solvent of the combined organic phases was removed under reduced pressure. The crude product was purified by column chromatography (silica gel, 1->5% MeOH in CH2CI2), to give 300 mg (41%) of 7-hydroxy-6-propionyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester (a 33/64 mixture of cis and trans isomers).
1H-NMR (CDCI3): 13.13-13.07 (m, 1H), 7.57-7.49 (m, 1H), 6.41-6.38 (m, 1H), 4.65-3.92 (m, 4H), 3.01-1.95 (m, 5H), 1.29-1.08 (m, 6H).
3d) (±)-c/s-7-Hydroxy-6-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
(Formula Remove)

±c/s-7-Hydroxy-6-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid was synthesized analogously to Example 2b from 7-hydroxy-6-

propionyi-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester (299 mg, 1.03 mmol, a 33/64 mixture of c/sand trans isomers), to give 39,3 mg (15%) of (±)-c/s-7-hydraxy-6-propionyl-1,1a,2,7b-tetrahydro-cyc!opropa[c]chromene-1 -carboxylic acid as a white solid and (±)-frans-7-hydroxy-6-propionyl-1,1 a^jb-tetrahydro-cyclopropafcjchromene-l -carboxylic acid as a byproduct. The crude product was purified by column chromatography (silica'gel, 'l-»5% MeOH in CH2CI2}.
1H-NMR (DMSO-de): 7.67 (d, 1H), 6.35 (d, 1H), 4.57 (dd, 1H), 4.36 (dd, 1H), 2.98 (q, 2H), 2.55-2.46 (m, 1H), 2.18-2.00 (m, 2H), 1.10 (t, 3H).
3e) (±)-c/s-1 -(5-Cyano-pyridin-2-yl)-3-(7-hydroxy-6-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea.
(Formula Remove)

(±)-c/s-1-(5-Cyano-pyridin-2-yl)-3-(7-hydroxy-6-propionyl-1,1a,2,7b-tetrahydro-cyc!opropa[c]chromen-1-yl)-urea was synthesized analogously to Example 1c from ±c/s-7-hydroxy-6-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (39.3 mg, 0.15 mmol). The crude product was purified by HPLC (Cis, 5-s-95% acetonitrile in H20), to give 2.9 rng (5.1%) of (±)-c/s-1-(5-cyano-pyridin-2-yl)-3-(7-hydroxy-6-propionyl-1,1a,2,7b-tetrahydro-cyc!opropa[c]chromen-1-yl)-urea.
1H-NMR (DMSO-d6): 13.15 (s, 1H), 9,71 (s, 1H), 8.30 (d, 1H), 8.01 (dd, 1H), 7.73 (d, 1H), 7.57 (d, 1H), 7.50 (d, 1H), 6.43 (d, 1H), 4.42 (dd, 1H), 4.13 (dd, 1H), 3.45-3.32 (m, 1H), 3.01 (q, 2H), 2.49-2.42 (m, 1H), 1.97-1.86 (m, 1H), 1.12{t, 3H).

±c/s-1 -(6-Acetvl-7-hvdroxv-1 , 1 a,2J7b-tetrahvdro-cycloproparclchromen-1 -vlV3-(5-cvano-Pvridin-2-vlKurea
4a) 1 -(2-Hydroxy-4-prop-2-ynyloxy-phenyl)-ethanone
(Formula Remove)

1-(2-Hydroxy-4-prop-2-ynyloxy-phenyl)-ethanone was synthesized
analogously to Example 3a from 1-(2,4-dihydroxy-phenyl)-ethanone (20 g,
131 mmol), to give 22 g (88%) of 1-(2-hydroxy-4-prop-2-ynyloxy-phenyl)-
ethanone.
1H-NMR (CDCI3): 12.70 (s, 1H), 7.66 (d, 1H), 6.52 (m, 2H), 4.72 (d, 2H), 2.58-
2.55 (m,4H).
4b) 1 -(5-Hydroxy-2H-chromen-6~y!)-ethanone
(Formula Remove)

o 1-(5-Hydroxy-2tf-chromen-6-yl)-ethanone was synthesized analogously to
Example 3b from 1 -(2-hydroxy-4-prop-2-ynyloxy-phenyl)-ethanone (17 g, 89 mmol), to give 6.0 g (35%) of 1-(5-hydroxy-2H-chromen-6-y!)-ethanone.
1H-NMR (CDC!3): 12.92 (s, 1H), 7.51 (d, 1H), 6.79 (dt, 1H), 6.32 (d, 1H), 5.71 (dt, 1H), 4.89 (dd, 2H), 2.55 (s, 3H).
4c) 6-Acetyl-7-hydroxy-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester

(Formula Remove)


6-Acetyl-7-hydroxy-1,1a,2,7b-tetrahydro-cyclopropa[cIchromene-1-carboxylic acid ethyl ester (a 40/60 mixture of ess and trans isomers) was synthesized analogously to Example Scfrom 1-(5-hydroxy-2H-chromen-6-yl)-ethanone.
1H-NMR (CDCI3): 13,05-12.97 (m, 1H), 7.54-7.47 (m, 1H), 6.43-6.33 (m, 1H), 4.63-3.94 (m, 4H), 3.02-1.96 (m, 6H), 1.31-1.08 (m, 3H).
4d) 6-Acetyl-7-hydroxy-1I1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid.
(Formula Remove)

0
6-Acetyl-7-hydroxy-il1a,2,7b-t6trahydro-cyclopropa[c]chromene-1-carboxylic acid was synthesized analogously to Example 1 b from 6-acetyl-7-hydroxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester (2 g, 8.1 mmol, a 40/60 mixture of as and trans isomers), to give 300 mg (17%) of 6-acetyi-7-hydroxy-1,1 a,2,7b-tetrahydro-cycloprapa[c]chromene-1 -carboxyfic acid (a. 40/60 mixture of cis and trans isomers). The crude product was purified by column chromatography (silica gel, 1-»5% MeOH in CH2CI2)
1H-NMR (CDC!3): 7.55-7.45 (m, 1H), 6.45-6.3Q (m, 1H), 4.65-4.00 (m, 2H), 3.05-1.95 (m,6H).
4e) (±)-c/5-1-(6-Acety[-7-hydroxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chrornen-1-yl)-3-(5-cyano-pyridin-2-yl)-urea
(Formula Remove)

0
(±)-c/s-1-(6-Acetyl-7-hydroxy-1l1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-cyano-pyridin-2-yl)-urea was synthesized analogously to Example to from 6-acetyl-7-hydroxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (300 mg, 1.21 mmol, a 40/60 mixture of cis and trans isomers).

The crude product was purified by HPLC (Cia, 5-»95% acetonitriie in HaO), to give 7.7 mg (17 %) of (±)-c/s-1-(6-acetyl-7-hydroxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-cyano-pyridin-2-yl)-urea and 9.0 mg (20 %) of (±)-fra/7s-1-(6-acetyl-7-hydroxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yi)-3-(5-cyano-pyridin-2-yl)-urea as a byproduct.
1H-NMR (CDCIa+CDa'OD): 7.98 (d, 1H), 7.74 (dd, 1H), 7.60 (d, 1H), 7.01 (d, 1H), 6.40 (d, 1H), 4.43 (dd, 1H), 4.29 (dd, 1H), 3.57 (dd, 1H), 2.69 (m, 1H), 2.61 (s, 3H), 2.00-1.86 (m, 1H).
Example 5
(+)-c/s-1-(5-Cvanopvridin-2-vl)-3-(7-fluoro-4-propionvl-1,1a12,7b-tetrahvdro-
cycloproparc1chrornen-1-vl)-urea.
5a) 1 -(4-Fluoro-2-prop-2-ynyloxy-phenyl)-propan-1 -one.
(Formula Remove)

To a mixture of NaH (95%, 278 mg, 11 mmol) in DMF (20 mL) at 0 °C, was added 1-(4-fiuoro-2-hydroxy-phenyl)-propan-1-one (1.68 g, 10 mmol) in DMF (5 ml). After 15 min at 0 °C, was 3-brorno-propyne (3.02 g, 20 mmol) added to the reaction mixture. After 1h at 0 °C, was the reaction mixture allowed to assume room temperature. The reaction mixture was extracted with HgO (100 ml). The H2O phase was washed with Et2O (3x100 ml) and the solvent of the combined organic phases was removed under reduced pressure. The crude product was purified by column chromatography (silica gel, CHaCfe), to give 1.40 g (68%) of 1-(4-fluoro-2-prop-2-ynyloxy-phenyl)-propan-1-one.
1H-NMR (CDCI3): 7.64 (dd, 1H), 6.69 (dd, 1H), 6.60 (ddd, 1H), 4.68 (d, 2H), 2.85 (q, 2H), 2.58 (t, 1H), 1.03 (t, 3H).

5b) 1-(5-Fluoro-2H-chromen-8-yl)-propan-1-one.
(Formula Remove)

1-(5-Fluoro-2/ir-chramen-8-yl)-propan-1-one was synthesized ana!agous!y to Example 3b from 1-(4-fluoro-2-prop-2-ynyloxy-pheny!)-propan-1-one (1.34 g, 6.5 mmol), to give 619 mg (46%) of 1-(5-f!uoro-2H-chromen-8-yI)-propan-1-one.
1H-NMR (CDCI3): 7.60 (dd, 1H), 6.67-6.58 (m, 2H), 5.86 (dt, 1H), 4.76 (dd, 2H), 2.93 (q,2H), 1.23 (t, 3H).
5c) (±)-c/s-7-F!uoro-4-propionyl-1 ,1a,2,7b-tetrahydro-cycfopropa[c]chromene-1 -carboxylic acid ethyl ester.
(Formula Remove)

"F
(±)-c/s-7-Fluoro-4-propiony!-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chramene-1 -carboxylic acid ethyl ester was synthesized according to method 3c) from 1 -(5-fluoro-2W-chromen-8-yl)-propari-1-one (619 mg, 3 mmoi), to give 142 mg (16%) of (±)-c/s-7-fluoro-4-propionyl-1,1a,2,7b-tetrahydro-cyc!opropa[c]chromene-1 -carboxylic acid ethyl .ester and (±)-frans-7-f tuoro-4-. propionyl-1 ,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester as a byproduct.
1H-NMR (CDC!3): 7.59 (dd, 1H), 6.65 (m, 1H), 4.50-4.46 (m, 2H), 3.95 (q, 2H); 2.89 (q, 2H), 2.57 (dd, 1H), 2.20 (dd, 1H), 1.13-1.03 (m, 1H), 1.12-1.01 (m,
6H).

5d) (±)-c/s-7-Fluoro-4-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid.
(Formula Remove)

F
(±)-c/s-7-Fluoro-4-propionyI-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid was synthesized analogously- to Example 1b-from (±)-c/s-7-f luoro-4-propionyl-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester (140.3 mg, 0.48 mmol), to give 83 mg (65%) of (±)-cis-7-f!uoro-4-propionyl-1 ,1 a,2,7b-tetrahydro-cyc!opropa[c]chromene-1 -carboxylic acid as a white solid. The crude product was purified by column chromatography (silica gel, 1-»5% MeOH in CH2CI2).
1H-NMR (DMSO-de): 12.15 (brs, 1H),7.46(dd, 1H),6.78(dd, 1H),4.57(dd, 1H), 4.43 (dd, 1H), 2.93-2.80 (m, 2H), 2.55 (dd, 1H), 2.24 (dd, 1H), 2.20-2.10 (m, 1H), 1.02(t,3H).
5e) (±)-c/s-1-(5-Cyanopyridin-2-yl)-3-(7-fluoro-4-propionyl-1 ,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea
(Formula Remove)

(±)-c/s-1 -(5-Cyanopyridin-2-yl)-3-(7-fluoro-4-propionyl-1 ,1 aj2,7b-tetrahydro-cyclopropa[c]chromen-1-y!)-urea was synthesized analagously to Example 1c from (±)-c/s-7-fluoro-4-propionyl-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (81 .9 mg, 0.31 mmol). The crude product was purified by HPLC (Cia, 5-»95% acetonitrile in H20), to give 12 mg (10%) of (±)-c/s-1-(5-cyanopyridin-2-yl)-3-(7-fluoro-4-propionyl-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea.
1H-NMR (DMSO-d6): 9.81 (s, 1H), 8.33 (d, 1H), 8.04 (dd, 1H), 7.83 (brs, 1H), 7.49-7.40 (m, 2H), 6.89 (dd, 1H), 4.41 (dd, 1H), 4.34 (dd, 1H), 3.46-3.38 (m, 1H), 2.76 (q, 2H), 2.56-2.46 (m, 1H), 2.09-1.98 (m, 1H), 0.93 (t, 3H).

Example 6
(±lcfe-1-(5-Cvano-pvridin-2-vn-3-(7-fiuoro-4-methoxy-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromen-1-yl)-urea.
6a) 6-F!uoro-2-hydroxy-3-methoxy-benzaIdehyde. 1M boron trichloride in dichlorometharte (25"ml; 25 mmol) was added to a" solution of 6-fluoro-2,3-dimethoxy-benzaldehyde [Cantreil, Amanda S.; Engelhardt, Per; Hoegberg, Marita; Jaskunas, S, Richard; Johansson, Nils Gunnar; et al; J.Med.Chem.; 39; 21; 1996; 4261-4274] (4.26 g; 23 mmoi) in dichloromethane (30 ml) keeping the reaction temperature at -70 C, The reaction mixture stirred at room temperature overnight and hydrolyzed with water. The organic phase was separated, washed with water and evaporated in vacua. The residue was chromatographed (silica gel, EA:Hex, 5:1) to give 3.72 g (94%) of 6-fluoro-2-hydroxy-3-methoxy-benzaldehyde as yellow crystals.
1H-NMR(CDC!3): 11.61 (s, 1H), 10.23 (s, 1H), 7.02 (dd, 1H), 6.55 (app.-t, 1H), 3.87 (s, 3H).
6b) 5-Fluoro-8-methoxy-2H-chromene.
6-Fluoro-2-hydroxy-3-methoxy-benzaldehyde (3.32 g, 19 mmol) was dissolved in acetonitrile (20 ml) and DBU (2.97 ml, 19 mmol) was added followed by -vinyltriphenylphosphine bromide (7.2 g, 19 mmoi). The reaction mixture was heated under reflux for48h, diluted with water and extracted with ether (3x50 ml), The organic phase was washed with water, 10% sodium hydroxide, water and brine and evaporated in vacuo. The residue was submitted to column chromatography (silica gel, EA:Hex, 1:20) yielding 1,2 g of 5-fluoro-8-methoxy-2H-chromene (34%).
1H-NMR (CDCla): 6.65 (m, 2H), 6.54 (t, 1H), 5.83 (dt, 1H), 4.88 (dd, 2H), 3.83(s, 3H).

6c) (±)-c/s-7-Fluoro-4-methoxy-1, i a,.;, / u-ieiranyaro-
cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
The title compound was synthesized analogously to example 3c from 5-fluoro-
8-methoxy-2H-chromene.
1H-NMR (CDCI3): 6.7-6.5 (m, 2H), 4.48 (m, 2H), 3.99 (m, 2H), 3.80 (s, 3H), 2:57-(app.t, 1H), 2.20 (app.t, 1H), 2.05 (m, 1H), i;08'(t; 3H).~
6d) (±)-c/s-7-Fluoro-4-methoxy-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
The title compound was synthesized analogously to example 1b from (±)-c/s-7-fluoro-4-methoxy-1,1 a, 2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester.
'H-NMR (CDCI3): 6.7-6.5 (m, 2H), 4.48 (m, 2H), 3.80 (s, 3H), 2.61 (app. t, 1H), 2.17 (app. t, 1H), 2.06 (m, 1H).
6e) (±)-c/s-1 -(5-Cyano-pyridin-2-yl)-3-(7-fluoro-4-methoxy-1,1 a,2,7bv
tetrahydro-cyc(opropa[c]chromen-1-yl)-urea.
The title compound was synthesized analogously to Example 1c from (±)-c/s-
7-fluoro-4-methoxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic
acid (62 mg, 0.17 mmol). Yield 38 mg (40%).
1H-NMR (CDCI3): 10.06 (br. s, 1H), 9.40 (br. d, 1H), 8.11 (d, 1H),7.70 (dd, 1H), 6.91 (d, 1H), 6.68 (m, 2H), 4.48 (dd, 1H), 4.28 (dd, 1H), 3.90-3.72 (m, 4H), 2.64 (app. T, 1H), 1.96 (m, 1H).

Example 7
f±)-c/s-1-(5-Cvano-pvridin-2-vl)-3-(7-f[uoro-4-chloro-1,ta,2,7b-tetrahvdro-
cvclopropafc]chrQmen-1-yi)-urea
7a) 1 -Chloro-4-fiuoro-2-prop-2-ynyloxy-benzene.
The title compound was synthesized analogously to example 15a) from 2-
chloro-5-ffuorophenol (2.5 g). Yield 2,8' g(9Q%). '""" ' '"'""" ..... ' '
'H-NMR (CDCI3): 7.32 (dd, 1H), 6.85 (dd, 1H), 6.68 (m, 1H), 4.77 (d, 2H), 2.58 (t,1H).
7b) 5-Fluoro-8-chloro-2H-chromene.
The title compound was synthesized analogously to Example 15b) from 1-
chloro-4-fluoro-2-prop-2-ynyioxy-benzene (2.8 g). Yield 0.97 g (35%).
1H-NMR (CDCI3): 7.09 (dd, 1H), 6.63 (dt, 1H), 6.56 (t, 1H), 5.84 (dt, 1H), 4.95 (dd, 2H).
7c)
carboxylic acid ethyl ester.
The title compound was synthesized analogously to Example 15c) from 5-F!uoro-8-chloro-2H-chromene.' • = ••-.-
1H-NMR (CDCI3): 7.14 (dd, 1H), 6.60 (t, 1H), 4.51 (m, 2H), 4.01 (m, 2H), 2.60( app. t, 1H), 2.23 (t, 1H), 2.09 (m, 1H), 1.08 (t, 3H).
7d) (iJ-c/s
1-carboxy(ic acid.
The title compound was synthesized analogously to example 1 5 d) from (±)-
c/s-7-fluoro-4-chloro-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chramene-1 -carboxylic
acid ethyl ester 850 mg). Yield 43 mg (96%).

1H-NMR (CDC!3): 8.86 (br. s, 1H), 7.13 (dd, 1H), 6.59 (t, 1H), 4.50 (m, 2H), 2.63 (t, 1H), 2.23-2.05 (m,2H).
7e) ±c/s-1 -(5-Cyano-pyridin-2-yl)-3-(7-fluoro-4-chIoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-y!)-urea.
The title compound was synthesized analogously to example 1c from (+J-cVs-7-fluoro-4-chloro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (63 mg). Yield 52 mg (56%).
1H-NMR (CDCI3): 9.79 (br. s, 1H), 9.34 (br. s, 1H), 8.22 (d, 1H), 7.72 (dd, 1H), 7.17 (dd, 1H), 6.87 (d, 1H), 6.67 (t, 1H), 4.54(dd, 1H), 4.33 (dd, 1H), 3.84 (app. q, 1H), 2.68 (dd, 1H), ZOO (m, 1H).
Example 8
±cis-1 -(5-Chloro-pyridin-2-yl)-3-f4-chloro-7-fluoro-1.1 a,2,7b-tetrahvdro-
cvcloproDafdchromene-l-vD-urea.
±cis-1-(5-Chloro-pyridin-2-yl)-3-(4-chloro-7-fluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-yl)-urea (15 mg, 24 %) was prepared according to the procedure described in example 1c, from ±cis-(4-chloro-7-fluoro-1,1a,2,7b-tetranydro-cyc!opropa[c]chromene)-1-carboxylic acid (40 mg, 0.16 mmol) arid 2-amino'-5-chloropyridine (76 mg, 0.57 mmol).
1H NMR (400 MHz,CDCI3) 5 ppm: 9.29 (brs, 1H), 9.26 (brs 1H), 7.84 (d, 1H), 7.47 (dd, 1H), 7.16 (dd, 1H), 6.76 (d, 1H), 6.67 (dd, 1H), 4.65 (dd, 1H), 4.34 (dd, 1H), 3.82 (dd, 1H), 2.62 (dd, 1H), 1.96 (m, 1H)

±c/s-1-(5-Bromo-pvridin-2-vl)-3-(4-chloro-7-fluoro-1,1a,2,7b-tetrahvdro-cyclopropa[c1chromene-1-vl)-urea.
±cis-1-(5-Bromo-pyridin-2-yl)-3-(4-chloro-7-fluoro-1,1a,2l7b-tetrahydro-cyclopropa[c]chromene-1-yl)-urea (13 mg, 19 %) was prepared according to the procedure described in example 1c, from ±cis-(4-chloro-7-fiuoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene)-1-carboxylic acid (40 mg, 0.16 mmol) and 2-amino-5-bromopyridine (99 mg, 0.57 mmol).
1H NMR (400 MHz, CDCI3) 5 ppm: 9.27 (brs, 1H), 9.02 (brs, 1H), 7.95 (d, 1H), 7.60 (dd, 1H), 7.16 (dd, 1H), 6.70 (d, 1H), 6.67 (dd, 1H), 4.50 (dd, 1H), 4.35 (dd, 1 H), 3.81 (dd, 1H), 2.63 (d.d, 1H), 1.97 (m, 1H)
Example 10.
±cis-1 -(5-Cvano-pvridin-2-yl)-3-(5-cvano-1.1 a.2.7b-tetrahydro-
cvclopropafc7chromen-1-y[)-urea
10a) Trifluoro-methanesulfonic acid 4-formyl-3-hydroxy-phenyl ester. A solution of triflic anhydride (1.77 ml, 10.5 mmol) in dichloromethane 10 ml) was added to a mixture of 2,4-dihydroxybenzaldehyde (1.38 g, 10 mmol) and pyridine (0.85 ml, 10,5 mmol) in dichloromethane (30 ml) at -70C. Dry ice bath was removed and the reaction mixture was stirred for 2h at room temperature. The reaction mixture was diluted with dichloromethane, washed with water, brine and evaporated in vacua. The crude product was purified by column chromatography (silica gel, EA:Hex, 1:6) to give 1.55 g of trifluoro-methanesulfonic acid 4-formy!-3-hydroxy-phenyl ester (57%).
1H-NMR (CDCI3): 11.28 (s, 1H), 9.93 (s, 1H), 7.67 (d, 1H), 6.95 (m, 2H).
10b) Trifluoro-methanesulfonic acid 3-alIy!oxy-4-formyl-phenyl ester. Potassium carbonate (1.6 g, 11.5 mmol) and allyl bromide (1 ml, 11.5 mmol) were added to a solution of trifluoro-methanesulfonic acid 4-formyl-3-hydroxy-

phenyl ester (1.55 g, 5.7 mmoi) in acetone (50 ml). The reaction mixture was stirred at 55C for 2h, filtered and evaporated in vacua. The residue was chromatographed (silica gel, EA:Hex, 1:20) to give 1.3 g (73%) of trifluoro-methanesulfonic acid 3-allyloxy-4-formyl-phenyl ester.
1H-NMR (CDCI3): 10.47 (s, 1H), 7.93 (d, 1H), 6.95 (d, 1H), 6.90 (s, 1H), 6.05
(m, 1H), 5:47 (d, 1H), 5.40 (d, 1H), 4.69 (d, 2H).- ' • •
10c) Trifluoro-methanesulfonic acid 3-allyloxy-4-vinyl-phenyl ester. Methyltriphenylphosphonium bromide (1.95 g, 5.45 mmol) was added to a suspension of sodium hydride (60% in oil) (0.25 g, 6.3 mmol) in THF (35 ml) at OC and it was stirred for 30 min at room temperature. To the above solution was added solution of trifluoro-methanesulfonic acid 3-aIlyloxy-4-formyl-phenyl ester (1.3 g, 4.2 mmol) in THF (15 ml), and the reaction mixture was stirred for 2h at room temperature. The reaction mixture was diluted with hexane and extracted with water. Organic phase was washed with brine and evaporated. Silica gel column chromatography (EA:Hex, 1:20) afforded trifluoro-methanesulfonic acid 3-aIlyloxy-4-vinyl-phenyl ester (0.68 g, 53 %).
1H-NMR (CDCI3): 7.51 (d, 1H), 7.02 (dd, 1H), 6.85 (dd, 1H), 6.77 (d, 1H), 6.05 (m, 1H), 5.76 (dd, 1H), 5.43(m, 1H), 5,32 (m, 2H), 4.58 (dt, 2H).
,1..0d) Trifluoro-methanesulfonic acid 2H-chromen-7-yl ester. To a solution of trifluoro-methanesulfonic acid 3-allyloxy-4-vinyl-phenyl ester (0.68 g, 2.2 mmol) in dichloromethane (5 ml) was added Ru-catalyst (Grubb's catalyst) (36 mg, 2 mol%), and the reaction mixture was stirred for2h at room temperature. After that period the reaction was complete (GC) and the reaction mixture was used in the next step without any work-up. Analytical sample was obtained after removal of the solvent by silica gel column chromatography (EA:Hex, 1:20).
1H-NMR (CDCI3): 6.97 (d, 1H), 6.76 (dd, 1H), 6.68 (d, 1H), 6.39 (dt, 1H), 5.81 (dt, 1H),4.98(dd,2H).

10e) ±c/s-5-Trifluoromethanesulfonyloxy-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylicacid ethyl ester. Rh(OAc)2 (19 mg, 2 mol%) was added to the above solution (10d) and the solution of EDA (0.44 ml, 4.4 mmol) in 1 ml of dichloromethane was added with a syringe pump over 5h at room temperature, When the reaction was complete (GC) dichloromethane was evaporated, the residue was dissolved in ethyl acetate and washed with saturated ammonium chloride solution and • ••-• brine. Organic phase was evaporated and crude mixture of cis- and trans-isomers (1:1.3) was separated by column chromatography (silica gel, EA:Hex, 1:6) to give 0.4 g (50%) of ±c/s-5-trifluoromethanesulfonyloxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
1H-NMR (CDCI3): 7,29 (d, 1H), 6.82 (dd, 1H), 6.73 (d, 1H), 4.51 (dd, 1H), 4.29 (dd, 1H), 3.98 (m, 2H), 2.45 (t, 1H), 2.19 (t, 1H), 2.05 (m, 1H), 1.03 (t, 3H).
10f) ±c/s-5-Cyano-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester.
±c/s-5-Trifluoromethanesulfonyloxy-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester (154 rng, 0.42 mmol), Pd(OAc)2 (9 mg, 10 mol%) and PPh3 (44 mg, 40 mol%) were mixed in DMF (4 ml) and gentle stream of nitrogen passed through reaction mixture for 10 min. Zn(CN)2 (74 mg, 0.63 mmol) was added, vial was sealed and the reaction mixture-was-stirred at 120C overnight. The reaction mixture was diluted with • ethyl acetate and extracted with saturated ammonium chloride. Organic phase was evaporated and residue chromatographed (silica gel, EA:Hex 1:5) to give 53 mg (52%) of ±c/s-5-cyano-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
1H-NMR (CDCIa): 7.33 (d, 1H), 7.19 (dd, 1H), 7.05 (d, 1H), 4.50 (dd, 1H), 4.25 (dd, 1 H), 3.99 (q, 2H), 2.46 (t, 1 H), 2.25 (t, 1 H), 2.11 (m, 1 H), 1.06 (t, 3H).

10g) ±c/s-5-Cyano-1,1a,2,7b-tetrahydro-cyciopropa[c]chromene-1-carboxylic acid.
±c/s-5-Cyano-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxyIic acid ethyl ester (53 mg, 0.22 mmol) and NaOH (35 mg, 0.88 mmol) were dissolved in mixture methanol water (1:1) (5 ml). Reaction mixture was stirred at 60C for 30 min. Methanol was evaporated in vacua and 20 ml of water was added. Resulting solution'was'extracted with ether. Water pfrase'Wasconcentrated,"" acidified with 1M HCI to pH~2 and extracted with ether. The organic phase was washed with brine and evaporated to give 42 mg (90%) of ±c/s-5-cyano-1,1 a,2,7b-tetrahydro-cyc!oprqpa[c]chromene-1 -carboxylic acid.
1H-NMR (CDClg): 7.33 (d, 1H), 7.19 (dd, 1H), 7.06 (d, 1H), 4.51 (dd, 1H), 4.31 (dd, 1 H), 2.53 (app. t, 1 H), 2.27 (app. t, 1 H), 2.16 (m, 1 H).
10h) ±c/s-1 -(5-Cyano-pyridin-2-yl)-3-(5-cyano-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea.
±c/s-5-Cyano~1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (42 mg, 0.19 mmol) and TEA (0.032 ml, 0.21 mmol) were dissolved in 3 ml of toluene. DPPA (0.046 ml, 0.21 mmol) and 2-amino-5-cyano-pirydine (25 mg, 0.21 mmol) were added. The reaction mixture was heated under reflux with stirring for 3h. The resulting precipitate was filtered and washed with hot ethanol (3 ml) yielding 41 mg (63%) of ±c/s-1-(5-cyano-pyridin-2-yl)-3-(5-cyano-1,1 a,2,7b-tetrahydro- cyclopropa[c]ch?6ffieh-1 -yl)-urea.
1H-NMR (DMSO-d6): 9.86 (s, 1 H), 8.48 (d, 1 H), 8.07 (dd, 1 H), 7.97 (br. s, 1 H), 7.51 (d, 1H), 7.43 (d, 1H), 7.37 (d, 1H), 7.34 (dd, 1H), 4.39 (dd, 1H), 4.19 (dd, 1 H), 3.57 (app. q, 1 H), 2.54 (app. t, 1 H), 2.09 (m, 1 H).

bxample 11
±c/s-1-(5-Cvano-pyridin-2-vn-3-(5-ethvnvl-1,1a.2.7b-tetrahvdro-
cvclopropafclchromen-1-vn-urea
11 a) ±c/s-5-Trimethylsilany!ethynyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester. .,. ±c/s-5-Trifluoromethanesulfonyloxy-1 ,.ta;2,7b-tetrahydr.o^, . cyc!opropa[c]chromene-1-carboxylic acid ethyl ester (152 mg, 0.41 mmol), DPPP (38 mg, 20 mol%), Pd(dba)2 (24 mg, 10 mol%), Cu! (3 mg, 4 mol%) were mixed in 3 ml of triethylamine and gentle stream of nitrogen passed through reaction mixture for 10 min. Trimethylsilyl-acetylene (0.088 ml, 0.62 mmol) was added, vial was sealed and the reaction mixture was stirred at 120C overnight. The reaction mixture was diluted with ethyl acetate, washed with water, brine and evaporated. The residue was purified by silica gel column chromatography (EA:Hex, 1:15) to give 0.1 g (77%) of ±c/s-5-trimethylsilanylethynyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
1H-NMR (CDCI3): 7.15 (d, 1H), 7.01 (dd, 1H), 6.88 (d, 1H), 4.47 (dd, 1H), 4.16 (dd, 1H), 3.96 (q, 2H), 2.38 (t, 1H), 2.13 (t, 1H), 2.01 (m, 1H), 1.04 (t, 3H), 0.22 (s, 9H).
11 b) ±c/s-5-Ethynyl-1 ,.1a,2,7b-tetrahydro-cyc!opropa[c]chromene-1-carboxylic acid.
±c/s-5-Trirnethylsilanylethynyl-1,1 a^Jb-tetrahydro-cyclopropalplchromene-l -carboxylic acid ethyl ester (0.1 g, 0.32 mmol) and sodium hydroxide (0.076 g, 1.9 mmol) were dissolved in mixture of methanohwater (1:1) (5 ml). The reaction mixture was heated at 6QC for 5h, then it was acidified with 1M HCl to pH~2 and extracted with ether. The organic phase was washed with brine and evaporated to give 66 mg (97%) of) ±e/s-5-ethynyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.

'H-NMR (CDCIa): 7.17 (d, 1H), 7.03 (dd, 1H), 6.91 (d, 1H), 4.45 (dd, 1H), 4.23 (dd, 1H), 3.02 (s, 1H), 2.46 (t, 1H), 2.13 (t, 1H), 2.07 (m, 1H).
11 c) ±c/s-1-(5-Cyano-pyridin-2-yl)-3-(5-ethynyl-1,1a,2,7b-tetrahydro-
cyciopropa[c]chromen-1-yl)-urea.
The title compound was synthesized analogously to example 10h from ±c/s-5-
ethynyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen.e-1-carboxylic acid. (6.6.mgr,
31 mmol).Yield 53 mg (52%).
1H-NMR (DMSO-ds): 9.88 (s, 1H), 8.41 (d, 1H), 8.06 (dd, 1H), 7.86 (br. s, 1H), 7.46 (d, 1H), 7.32 (d, 1H), 7.02 (dd, 1H), 6.93 (d, 1H), 4.31 (dd, 1H), 4.16 (dd, 1H), 4.12 (s, 1H), 3.47 (q, 1H), 2.43 (app. t, 1H), 2.00 (m, 1H).
Example 12
±c/s-1 -(5-Acetyl-1,1 a.2,7b-tetrahvdro-cyclopropafc1chromen-1 -vl)-3-(5-cvano-
pyridin-2-vl)-urea
12a) ±CAs-5-Acetyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
±c/s-5-Trifluoromethanesulfonyloxy-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester (117 mg, 0.32 mmol), DPPP (7.3 mg, 50 mol%), Pd(OAc)2 (2 mg, 25 mol%) and triethyl amine (0.09 ml, 0.64>mmol) were mixed in DMF (3 ml) and gentle stream of nitrogen... passed through reaction mixture for 10 min. Butyl vinyl ether (0.21 ml, 1.6 mmol) was added, vial was sealed and the reaction mixture was stirred at 100C for 2h. 5% HCI (5 ml) was added and the reaction mixture was stirred at room temperature for 30 min. Resulting mixture was extracted with ethyl acetate. The organic phase was washed with saturated ammonium chloride and evaporated. The residue was purified by silica gel column chromatography (EA:Hex, 1:5) to give 76 mg (91%) of ±c/s-5-acetyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.

'H-NMR (CDCI3):7.52 (dd, 1H), 7.36 (d, 1H), 7.34 (d, 1H), 4.51 (dd, 1H), 4.21 (dd, 1H), 3.98 (q, 2H), 2.53 (s, 3H), 2.47 (t, 1H), 2.23 (t, 1H), 2.08 (m, 1H), 1.05(t, 3H).
12b) ±c/s-5-Acety!-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic
acid.
Thq. title compound vyas synthesized analogously to, example, !Og,from;±c/s-5-,
acetyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl
ester (76 mg, 29 mmoi).Yield 66 mg (97%).
1H-NMR (CDCI3): 7.52 (dd, 1H), 7.37 (d, 1H), 7.34 (d, 1H), 4.52 (dd, 1H), 4.26 (dd, 1H), 2.55 (s, 3H), 2.53 (t, 1H), 2.25 (t, 1H), 2.13 (m, 1H).
12c) ±c/s-1 -(5-Cyano-pyridin-2-yl)-3-(5-acetyl-1,1 a,2,7b~tetrahydro-
cyc/opropa[c]chromen-1-yf)-urea.
The title compound was synthesized analogously to example 10h from ±c/s-5-
acety!-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (66 mg,
28 mmol).Yieid 58 mg (59%).
1H-NMR (DMSO-d6): 9.87 (s, 1H), 8.42 (d, 1H), 8.05 (dd, 1H), 7.88 (br. s, 1H), 7.52 (dd, 1H), 7.49-7.44 (m, 2H), 7.37 (d, 1H), 4.39 (dd, 1H},4.18 (dd, 1H), 3.55 (q, 1H), 2,55-2.50 (m, 4H, superimposed on residua! DMSO-d6 peak), 2.07 (m, 1H).
Example 13
±c;s-1 -(5-Methoxy-l ,1 a,2,7b-tetrahvdro-cvclopropafc1chrorri6n-1 -vO-3-(5-
cyano-pvridin-2-vl)-urea
The title compound was synthesized analogously to example 10 from 2-hydroxy-4-methoxybenzaldehyde.

'H-NMR(CDCI3):8.44(br. s, 1H), 8.06(d, 1H),7.70(dd, 1H), 7.18 (d, 1H), 6.82 (br. d, 1H), 6.55 (dd, 1H), 6.36 (d, 1H), 4.32 (dd, 1H), 4.24 (dd, 1H), 3.76 (s, 3H), 3.58 (q, 1H), 2.36 (dd, 1H), 1.86 (m, 1H).
Example 14
±cis-1 -(5-Cvano-pvridin-2-yl)-3-(A/-acetvl-1,1 a.3,7b-tetrahvdro-2-oxa-
cyclopropafalquinoline-1 -vl))-urea .,,t-,-- ,...„..,.,.-. .......
a) /V-Acetyl-1,2-dihydroquinoline.
Quinoline (19.37g, 150 mmol) was dissolved in anhydrous diethyl ether (500 ml) and cooled to 0 °C under inert atmosphere. DIBAL, 1.5 M in toluene (100 ml, 150 mmol) was added dropwise over 2 hrs and the reaction mixture was stirred at 0 °C for 30 min. Acetic anhydride (500 ml) was added dropwise over 30 min and the reaction mixture was stirred at 0 °C for 30 min. HgO was added cautiously. The reaction mixture was extracted with diethyl ether and concentrated to give A/-acetyl-1,2-dihydroquinoline (11.5 g, 44 %).
b) ±cis-(A/-acetyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]quinoline)-1 -
carboxylic acid ethyl ester.
±cis-(A/-acetyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]quinoline)-1-carboxylic acid ethyl ester was prepared according to the procedure described in example 1a, frorrt /V~acetyl-1,2-dihydroquinoline (10 g, 58 mmol) The product was purified by column chromatography on silica (EtpAc/hexane 5%->50%) to give ±cis-(A/-acetyl-1,1 a^./b-tetrahydro-cyclopropafclquinolinej-l-carboxylic acid ethyl ester (2.0 g, 13%).
c) ±cis-(A/-Acetyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]quinoline)-1 -
carboxylic acid.
±cis-(A/-AcetyI-1,1al2,7b-tetrahydro-cyclopropa[c]quinoline)-1-carboxylic acid (425 mg, 24 %) was prepared according to the procedure described in example 1b, from ±cis-(/V-acetyl-1,1a,2,7b-tetrahydro-cyclopropa[c]quinoline)-1-carboxylic acid ethyl ester (2.0 mg, 7.7 mmol) .

d) ±cis-1 -(5-Cyano-pyridin-2-yl)-3-(/V-acety!-1,1 a,3,7b-tetrahydro-2-oxa-
cyclopropa[a]quinoline-1-yl))-urea.
±cis-1 -(5-Cyano-pyridin-2-yl)-3-(W-acetyl-1, 1a,3,7b-tetrahydro-2-oxa-
cyclopropa[a]quinoline-1-yl))-urea (250 mg, 40 %) was prepared according to
the procedure described in example 1c, from ±cis-(A/-acetyl-1,1a,2,7b-
tetrahydro-cyclopropa[c]quinoline)-1-carboxylic acid (416 mg, 1.8 mmol).
1H NMR (250 MHz, DMSO-d6) 5 ppm: 9.51 (brs, 1H), 8.30 (d 1H), 8.01 (dd, 1H), 7.54 (dd, 1H), 7.44, (dd, 1H), 7.36 (d, 1H), 7.23-7.18 (m, 3H), 4.10 (d, 1H), 3.60 (dd, 1H), 3.12-3.05 (m, 1H),2.37(tr, 1H), 2.0-1.92 (m, 4H)
Example 15
+/--c/'s-1-(5-Cvanopvridin-2-v()-3-(4,7-difluoro-1,1a,2,7b-tetrahvdro-
15 a) 2,4-Difluoro-2-propynyIoxybenzene.
cvcloproparc1chrornen-1-yl)-urea
(Formula Remove)

(Formula Remove)

Commercially available 2,5-difIuorophenol (20 g , 0.15 mol), K2CO3 (53 g, 0.38 mol) and commercially available 3-bromopropyne (45 g, 0.38 mol) were dissolved in acetone (300 ml), refluxed over night, cooled and filtrated. The solvent was removed and the crude product, dissolved in ether and washed with water and brine. The organic phase was evaporated and the crude product was re-dissolved in a small amount of ether and filtrated through a column of basic AI2O3. Evaporation and drying gave20 g (80 %) of 2,4-difluoro-2-prop-ynyloxy-benzene

15b) 5,8-umuoro-k!rt-cnromene.
(Formula Remove)

2,4-Difluoro-2-propynyloxybenzene (20 g, 0.12 mol) was dissolved in N,N,-diethyl aniline (100 ml) and heated under argon atmosphere at 225 deg. Celcius with an oil-bath for 6- 8 h. Ether (150 ml) was added and the aniline was removed by extraction using 2 M HCI(aq). Purification bychromatography (silica gel, n-hexane) gave 5,8-difluoro-2H-chromene 5.8 g (29 %)
15c) +/- c/s-4,7-Difluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester.
(Formula Remove)

5,8-Difluoro-2H-chromene (5 g, 0.03 mol), (Rh(ll)Ac2)2 (0.39 g, 0.00089 mol) was dissolved in 1,2-dichloroethane (60 ml) or ethanol-free chloroform. Ethyl diazoacetate (9.4 ml, 0089 mol) in the same solvent was added dropwise over a period of approximately 5 h under N2 atmosphere. The solvent was then removed under vacuum and the mixture was taken upp in ethyl acetate, washed with NaHCC>3(aq), water and brine and the solvent removed. The product (33 % cis, 66 % trans) was purified by hromatography (0 -M 0 % • ethyl acetate in n-hexane) to give 2.2 g of the title compound ( 30 %).
1 5d) c/'s4,7-Difluoro-1 , 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
(Formula Remove)

C/s-4,7-Difluoro-1 , 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester (2 g, 0.008 mol) was heated in 1 M LiOH in methanol-water (25

%) at 80 deg. for 2 h. The volume was reduced to half and acidified. Extraction with ether followed by chromatography (silica gel, ether) gave pure title compound (35 %)
e) (/-)c/s-1 -(5-Cyano-pyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)-urea
(Formula Remove)

(+/-)-c/s-1 -(5-Cyano-pyridin-2-yl)-3-(4,7-difluoro-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea was prepared analogously to Example 1 c but using c/s-4,7-dif!uoro-1 , 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (0.2 g, 0.00088 mol) to give 0.130 g (42 %) of pure title compound. The crude product was purified by extraction between 0.01 M HCI (aq) and ethyl acetate and chromatography (silica gel, 0-»1 % MeOH in Ether). The solvent was evaporated and the solid washed with a cold solution of 50 % aceton in n-hexane.
1H-NMR (CDCI3-MeOD): 8.16 (d, 1H), 7.72 (dd, 1H), 6.97-6.86 (m, 2H), 6.69-6.61 (m, 1H), 4.47 (dd, 1H), 4.31 (dd, 1H), 3.75 (m, 1H), 2.65 (t, 1H), 2.05-1.96(m,
Example 1 6
(+/-)-c/s-1-(5-Gvano-3-methyl-pvridin-2-vn-3-(4,7-difluoro-1,1a,2.7b-
(+/-)-c/s-1 -(5-Cyano-3-methyl-pyridin-2-yl)-3-(4,7-dif luoro-1,1 a,2,7b-tetrahydro-cyc!opropa[c]chromen-1-yl)-urea was prepared analogously to
tetrahydro-cyclopropafclchromen-l-vD-urea
(Formula Remove)


Example 1c but using c/s-4,7-Difluoro-1, 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (168 mg, 0.74 mmol) and 6-amino-5-methyl-nicotinonitrile (109 mg, 0.82 mmol) to give (+/-)-c/s-1 -(5-cyano-3-methyl-pyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea 52 mg of(20 %). The crude product was purified by extraction between 0.01 M HCI (aq) and ethyl acetate and chromatography (silica gel, 0->25% MeOH in Ether). The. solvent was. . . evaporated and the solid washed with 25 % aceton in n-hexane.
1H NMR (CDCI3-MeOD): 8.02 (d, 1H), 7.61 (dd, 1H), 6.97-6.87 (m, 1H, 6.70-6.62 (m, 1H), 4.48 (dd, 1H), 4.30 (dd, 1H), 3.78 (t, 1H), 3.37 (s, 3H), 2.66 (t, 1H),2.03(m, 1H).
Example 17
+/--C/S-1 -(5-Chloro-pyridin-2-vn-3-f4.7-difluoro-1.1 a.2.7b-tetrahvdro-
cvclopropafc'lchromen-1 -vl)-urea
(Formula Remove)

F
+/--c/s-1-(5-Chloro-pyridin-2-yl)-3-(4,7-difluoro-1I1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea was prepared analogously to Example 1c but using c/s-4,7-dif!uoro-1, 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (90 mg, 0.4 mmol) and 6-amino-5-chloropyridine (51. mg, 0.44 , mmol) to give +/--c/s-1-(5-chloro-pyridin-2-yl)-3-(4,7-difluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea (50 mg, 35 %). The crude product was purified by extraction between 0.01 M HCI (aq) and ethyl acetate-ether (1:1) and chromatography (silica gel, ether).
1H NMR (CDCl3): 9.2 (broad s, NH), 8.6 (broad s, NH), 7.81 (dd, 1H), 7.48 (dd, 1H), 6.89 (m, 1H), 6.75 (d, 1H), 6.69 (m, 1H), 4.45 (dd, 1H), 4.33 (dd, 1H), 3.75 (m, 1H), 2.61 (m, 1H), 1.97 (m, 1H).

Example 18
(+/-)-c/s-1-(417-Difluoro-1.1a,2,7b-tetrahvdro-cycloproparc1chromen-1-vl)-3-(5-
ethvnv(-pvridin-2-yl)-urea
(Formula Remove)

F
+/- c/s-1 -(4,7-Difluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-ethynyl-pyridin-2-yl)-urea was prepared analogously to Example 1c) but using c/s-4,7-difluoro-1, 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (100 mg, 0.4 0.44 mmol) and 5-trimethylsilanylethynyl-pyridine-2-y!amine (93 mg, 0.49 mmol) to give (25 mg, 17 %). The crude product was purified by extraction between 0.01 M HCI (aq) and ethyl acetat-ether (1:1) and chromatography (silica gel, ether). The mixture obtained (containing the title compound together with silylated compound) was stirred with BU4N+F" in 25 %water in THF for 30 min and the chromatography was repeated to obtain pure +/- c/s-1 -(4,7-Difluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]ch.romen-1 -yl)-3-(5-ethynyl-pyridin-2-yl)-urea.
1H NMR (CDCI3): 9.2( broad s, NH), 7.95 (d, 1H), 7.59 (dd, 1H), 7.48 (broad s, 1 H) , 6.89 (td, 1 H), 6.64 (td, 1 H), 6.57 (d, 1 H), 4.46 (dd, 1 H), 4.33 (dd, 1 H), 3.78 (q, 1H),3.11 (s, 1H), 2.62 (t, 1H), 1.99-1.97 (m, 1H)
Example 19
(+/-)-c/s-1-(5-Bromo-pvridin-2-vl)-3-(4.7-difluoro-1.1a.2.7b-tetrahvdro-
cv_cjoproparclchromen-1-vl)-urea
(Formula Remove)

•4-/--c/s-1-(5-Bromo-pyridin-2-y!)-3-(4,7-dif]uoro-1,1a,2,7b-tetrahydro- . cyclopropa[c]chrornen-1-yl)-urea was prepared analogously to Example 1c but using c/s-4,7-difluoro-1, 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-

carboxylic acid (50 mg, 0.22mmol) and 6-amino-5-bromopyridine (42 mg, 0.24 mmol) to give +/--c/s-1-(5-brorno-pyridin-2-yI)-3-(4,7-difluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chrornen-1-yl)-urea (50 mg, 35 %). The crude product was purified by extraction between 0.01 M HC! (aq) and ethyl acetate and chromatography (silica gel, ether.
1H NMR ,(CDCI3): 9.2 (broad s, NH), 7.88 (d, 1H), 7.75 (broad s, 1H), 7.60 (dd, 1H), 6.89 (m, 1H), 6.63 (td, 1H ), 6.59 (d, 1H), 4.45 (dd, 1H), 4.33 (dd, 1H), 3.78 (q, 1H), 2.62 (t, 1H), 1.98 (m, 1H).
Example 20
-*-/- c/s-1 -(4.7-Difluoro-1,1 a,2,7b-tetrahvdro-cycloproparc1chromen-1 -yl)-3-(5-
phenoxv-pvridin-2-vn-urea.
(Formula Remove)

(+/-)-c/s-1 -(4,7-Difluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-phenoxy-pyridin-2-yl)-urea was prepared analogously to Example 1c) but using c/s-4,7-difluoro-1, 1a,2,7b-tetrahydro-cyclopropa[c]chfomene-1-carboxylic acid (60 mg, 0.26 mmol) and 6-amino-5-phenoxypyridlne (56 mg, 0.29 mmol) to give 32 mg (30 %) of the title compound. The crude product was purified by extraction between 0.01 M HCI (aq) and ethyl acetate and chromatography (silica gel, 20 % ether in n-hexane)
1H NMR (CDCI3): 7.60 (d, 1H), 7.45 (broad s, 1H), 7.37-7.34 (m, 2H), 7.27-7.24 (m, 2H), 7.14-7.11 (m, 1H), 6.94-9.92 (m, 2H), 6.79-7.74 (m, 1H), 6.63 (d, 1 H), 6.59-6.55 (m, 1 H), 4.43 (dd, 1 H), 4.36 (dd, 1 H), 3.75 (q, 1 H), 2.59 (t, 1H), 1.98-1.94 (m, 1H).

Example 21
(+/-)-c/s-1 -(5-Cyanq-pyridin-2-yl)-3-(4.7-difluoro-1.1 a,2.7b-tetrahydro-
cyciopropafclchromen-1-vlHhjQurea
(Formula Remove)

-
c/s-4,7-Difluorb-1, 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (113 mg, 0.5 mmol), DPPA (118.6 jil, 0.55 mmol) and TEA (70.7 nJ, 0.55 mmol) was refluxed in toluene (2ml) for 1 h. Dioxane (3 ml) and HCI(aq) (1.5 ml, 6M) was then added and the reaction mixture was left for 1 h. at 50 °C. Ether and water was then added and the layers separated. The water phase was washed with ether and then made alkaline with ammonia (aq). Extraction with dichlorometane and drying gave the intermdiate 4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-1yiamine, which was directly treated with 6-isothiocyanato-nicotinonitril (34 mg, 0.55 mmol) in acetonitrile (4 ml) at RT over-night. The precipitated crystals were filtrated off and washed with cold acetonitrile to giveSO mg (17 %) of pure (+/-)-c/s-1-(5-Cyano-pyridin-2-y!}-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-cyciopropa[c]chromen-1 -yl)-thiourea LC-MS: m/z 358.9
Example 22
1 -(6-ChJoro-5-cyano-Pvridin-2-vn-3-(5,7-difluorQ-1.1 a,2,7b-tetrahydro-
cvclopropaTc]chromen-1-yl)ure_a

Cl
(Formula Remove)
1 -(6-Chloro-5-cyano-pyridin-2-yl)-3-(5,7-difluoro-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)urea was prepared analogously to Example 1c) but using c/s-5,7-difluoro-1, la^Jb-tetrahydro-cyclopropatcjchromene-l-carboxylic acid (280 mg, 1.21 mmol) and 6-amino-2-chloro-3-cyanopyridine ( 203 mg, 1 .33 mmol) to give the title compound in small amount. The crude product was purified by extraction between 0.01 M HCI (aq) and ether and chromatography (silica gel, ether ) and washed with acetone-ether.

1H NMR (DMSO-d6): 1 0 (br s, NH), 8.20 (d, 1 H), 7.70 (d, 1 H), 6.9 (br s, NH),
6.8 (m, 1 H), 6.6 (m, 1 H), 4.4 (dd, 1 H), 4.2 (dd, 1 H), 3.2 (m, 1 H), 2.4 (t, 1 H),
1.8 (m, 1H).
Example 23
1 -(5-cvano-pyridin-2-vl)-3-(5,7-difluoro-1 .1 a,2.7b-tetrahydro-
cyclopropa[clchrornen-1-vl)urea
(Formula Remove)

1 -(5-cyano-pyridin-2-yl)-3-(5,7-difluoro-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)urea was prepared analogously to Example 1c) but using c/s-5,7-difluoro-1 , 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (390 mg, 1 .72mmol) and 2-amino-5-cyanopyridine ( 226 mg, 1 .89 mmol) . The crude product was purified by extraction between 0.01 M HCI (aq), recrystallization, several washings with aceton and acetonitrile and chromatography (silica gel, 1 % EtOAc in ether) to give 28 mg of the title compound.
1H NMR (CDCI3-MeOD): 8.16 (t, 1H), 7.78 (dd, 1H), 7.09 (d, 1H), 6.56-6.34 (m, 2H), 4.34 (m, 2H), 3.54 (t, 1H), 2.57 (dd, -1H), 2.00-1.90 (m, 1H).
Example 24
c/s-1 -(4-Bromo-7-f luoro-1 .1 a,2,7b-tetrahvdro-cvclopropafc1chrQmen-1 -yl)-3-(5-
(Formula Remove)
cyano-pyridin-2-yl)-urea

I ~
NH HN— N- '
c/s-1 -(4-Bromo-7-fIuoro-1)1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-cyano-pyridin-2-yl)-urea was prepared analogously to Example 1c) but using c/s-4-bromo-7f luoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (178 mg, 0.62 mmol) and 2-amino-5-cyanopyridine (0.81 mg, 0.68 mmol)

The crude product was chromatographed (silica, ether) and washed with acetone to give 40 mg (16%) of c/s-1-(4-Bromo-7-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-cyano-pyridin-2-yl)-urea.
1H NMR (CDC!3): 9.85 (s, 1H), 9.3 (s,1H), 7.75 (dd, 1H), 7.33 (dd, 1H), 6.95 (d, 1H), 6.65 (t,1H), 4.05 (dd, 1H), 4.32 (dd, 1H), 3.35 (t, H), 2.65 (t, 1H), 2.05-1.95(m, 1H).
Example 25
c/s-1 -(4-Br_omo-7-f luoro-1,1 a.2 Jb-tetrahydro-cycloproparclchrornen-1 -ylV3-(6-
chloro-5-cyano-pvridin-2-vl)-urea
(Formula Remove)

c/s-1-(4-8romo-7-f luoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(6-chIoro-5-cyano-pyridin-2-yl)-urea was prepared analogously to Example 1c but using c/s-4-bromo-7fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (178 mg, 0.62 mmol) and 2-amino-6-chloro-5-cyanopyridine ( 105 mg, 0.68 mrnol). The crude product was chromatographed (silica, 0-»1 % MeOH in ether) and washed with acetone-hexarie to give 40 mg (13 %) of c/s-1 -(4-bromo-7-f iuoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)-3-(6-chloro-5-cyano-pyridin-2-yl)-urea,
1H NMR (CDCIa): 9.90 (s, 1H), 8.30 (s, 1H), 7.75 (d, 1H), 7.25 (d, 1H), 6.60 (t, 1H),4,5 (dd, 1H),4.35(dd, 1H), 3.5 (m, 1H), 2.65 (m, 1H), 2.1-1.95 (m, 1H).

Example 26
c/s-1-(4-Bromo-6-fluoro-1.1a,2,7b-t6trahvdro-cycloproparc1chromen-1-vl)-3-(5-
cyano-pvridin-2-vl)-iirea
o
c/s-1 -(4-Bromo-6-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1 -yl)-3-(5-cyano-pyridin-2-yl)-urea urea was prepared analogously to Example 1c but using c/s4-bromo-6-fluoro-1Ja,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (177 mg, 0.62 mmol) and 2-amino-5-cyanopyridine (81 mg, 0.68 mmol). The crude product was extracted between ether and 0.02 M HCI(aq), chromatographed (silica, 0->1 % MeOH in ether) and washed with acetone-hexane to give 42 mg (17 %) of c/s-1 -(4-Bromo-6-fluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(5-cyano-pyridin-2-yl)-urea.
1H NMR (CDCI3-MeOD): 8.37 (m, 1H), 7.75 (dd, 1H), 7.14 (dd, 1H), 7.05 (dd, 1H), 6.93 (d, 1H), 4.56 (dd, 1H), 4.21 (dd, 1H), 3.77 (t, 1H), 2.42 (dd, 1H), 2.00 (m, 1H).
Example 27
c/s-1 -(4-Bromo-6-fluoro-1.1 a,2.7b-tetrahvdro-cvcloproparc]chromen-1-vn-3-(6-
cloro-5-cvano-pyridin-2-vl)-urea
(Formula Remove)

F
c/s-1 -(4-Bromo-6-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-y!)-3-(6-ch!oro-5-cyano-pyridin-2-yl)-urea was prepared analogously to Example 1c) but using c/s^-bromo-6-f!uoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (177 mg, 0.62 mmol) and 2-amino-6-chloro-5-cyanopyridine (105 mg, 0.68 mmo!). The crude product was extracted between ether and 0.01 M HCI(aq) , chromatographed (silica, 0->1 % MeOH in ether) and washed

with acetone-hexane to give 46 mg (17 %) of c/s-1-(4-bromo-6-fluoro-
1,1a,2J7b-tetrahydro-cyclopropa[c]chromen-1-yl)-3-(6-cloro-5-cyano-pyridin-2-
yi)-urea.
1H NMR (CDCI3): 9.41 (s1H,), 8.28 (dd, 1H), 7.04 (dd, 1H), 4.54 (dd, 1H), 4.25 (dd, 1H), 3.50 (m, 1H), 2.41 (dd, 1H), 2.06-1.98 (m, 1H).
Example 28
C/s-1 -(5-cvanopvridin-2-vl)-3-(6-fluoro-1.1 a.2.7b-tetrahydro-
cvcloproparclchromen-1 -ypurea
(Formula Remove)

F
c/s-1 -(5-Cyano-pyridin-2-yl)-3-(6-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)urea was prepared analogously to Example 1c) but using c/s-6-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (168 mg, 0.8 rnmol) and 2-amino-5-cyanopyridine ( 105 mg, 0.88 mmol). The crude product ,was extracted between ether and 0.01 M HCI(aq) chromatographed (silica, 0-»1 % MeOH in ether) and washed with aceton-hexane to give only 10mg (4 %) of c/s-1-(5-cyano-pyrfdin-2-yl)-3-(6-fluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)urea.
1H NMR (CDCI3-MeOD): 8.16 (d, 1H), 7.73 (dd, 1H), 7.05 (dd, 1H), 6.96 (d, 1H), 6.84 (td ,1H), 6.76 (dd, 1H), 4.39 (dd, 1H), 4.17 (dd, 1H), 3.67 (t, 1H), 2.39 (dd, 1H), 1.96-1.92 (m, 1H).

Intermediates
29a) 6-Fluorochroman-4-ol
(Formula Remove)




6-Fluorochroman-4-one (10 g, 61 mmol) was dissolved in ethanol (100 ml). NaBH4 (excess) was added and cooled on icebath. The mixture was then left in room temperature for 2h, .folowed by reflux for 4 h. Purification by chromatography (silica gel, ether-hexane, 1:5) gave 8. g (80 %) pure 6-fluoro-chroman-4-ol.
29b) 6-F!uoro-2H-chromene
(Formula Remove)

F 6-F!uorochroman-4-ol (8 g, 48 mmol) and toluene-4-sulphonic acidn (1g) were
dissolved in toluene and refluxed over-night, with subsequent water removal. The mixture was then cooled and washed with NaHCOa (aq) and purified by chromatography (silica gel, n-hexane) to give 4.2 g (52 %) of pure 6-fluoro-2H-chromene.
29c) •f/-cis-6-Fluoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester
-^**^
.COzEt
(Formula Remove)


This Compound was prepared analogously to c/s-4,7-Difluoro-1, 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxyiic acid ethyl ester but using 6-fiuoro-2H-chromene to give 1.9 (29 %) of the title compound.
29d) C/s-6-Fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxyiic acid

COM
(Formula Remove)
F
This compound was prepared analogously to c/s-4,7-difIuoro-1 , 1 a,2,7b-tetrahydro-cyc!opropa[c]chromene-1 -carboxyiic acid but using cis-6-fiuoro-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxyiic acid ethyl ester (1 .9 g, 8 mmol) to give 350 mg (21 %) of pure c/s-6-fluoro-1 , 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxyiic acid
29e) 1 -Bromo-4-f]uoro-2-prop-2-ynyloxy-benzene

Br-
(Formula Remove)
F
This compound was prepared analogously to 2,4-difluoro-2-prop-ynyloxy-benzene but using 2-bromo-5-fiuorphenol (15 g, 78 mmol) to give 1-bromo-4-fluoro-2-prop-2-ynyloxy-benzene 15.6 g (87 %)
29f) 2-Bromo-4-fluoro-1 -prop-2-ynyloxy-benzene
(Formula Remove)

This compound was prepared analogously to 2,4-difluoro-2-prop-ynyloxy-benzene but using 2-bromo-4-fluoro-phenol (15 g, 78 mmol) to give 2-bromo-4-fluoro-1-prop-2-ynyioxy-benzene 15. g (84%).

29g) 1,3-dif!uoro-5-prop-2-ynyloxy-benzene
This compound was prepared analogously to 2,4-difluoro-2-propynyloxybenzene but using 3,5-difiuoro-phenol (1.4 g, 107 mmol) to give, 1,3-difluoro-5-prop-2-ynyloxy-benzene 12 g (67 %).
9h) 8-Bromo-6-fluoro-2H-chromene
(Formula Remove)




This compound was prepared analogously to 5,8-difluoro-2/-/-chromene but using (15 g, 65 mmol) of 2-bromo-4-fluoro-1-prop-2-ynyloxybenzeneto give the title compound (7 g, 46 %)
29i) 8-Bromo-5-fiuoro-2H-chromene
(Formula Remove)

F
This compound was prepared analogously to 5,8-difluoro-2H-chromene but using- (15 g, 65 mmol) of 1-bromo-4-fluoro-2-prop-2-yny!oxybenzeneto give the title compound (3.7 g, 25
29j) 5J7-Difluoro-2/-/-chromene
(Formula Remove)

F
This compound was prepared analogously to 5,8-difluoro-2f7k;hromene but using (18 g, 107 mmol) of 1,3-difiuoro-5-prop-2-yny!oxybenzene and PEG-200 as solvent to give the title compound (4 g, 23 %).

29k,) +/- c/s-4-Bromo-6-fluoro-1, 1a,2,7b-tetrahydro-cyc!opropa[c]chromene-1 -carboxylic acid ethyl ester

(Formula Remove)


F
This compound was prepared analogously to +/- c/s-4,7-dif!uoro-1 , 1a,2,7b-tetrahydro-cycfopropa[c]chromene-1 -carboxylic acid ethyl ester but using" 5" g (22 mmol) of 8-bromo 6-fluoro-2/ir-chromene to give 1 .9 g (30 %) of cis-6-fluoro-1 , 1 a,2, 7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester.
29!) +/- c/s^)-Bromo-7-fluoro-1 , 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester
(Formula Remove)

.coaa
F This compound was prepared analogously to +/- ds^,7-difluoro-1,1a,2,7b-
tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester but using 3.5 g (15.3 mmol) of 8-bromo-5-fluoro-2Afchromene to give 1.6 g (33 %) of +/-c/s-4-bromo-7-fluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
29rn) .+/-.c/s-5,7-Dif!uoro-1., 1a,2,7b-tetrahydro-cyclopropa[c]chromene.-.1- . carboxylic acid ethyl ester.
(Formula Remove)

F
This compound was prepared analogously to +/- c/s-4,7-difiuoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic .acid ethyl ester but using 2 g (12 mmol) of 5,7-difiuoro-2/-/-chromene to give 0.9 g (29 %) of +/- c/s-5,7-difluoro-1, 1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.

Example 30
Optical isomers of c/'s-1-(5-Cvano-pvridin-2-yn-3-(4.7-difluoro-1,1a.2.7b-
tetrahydro-cvclopropafclchromen-l-vD-urea
Racemic (+/-)-c/s-1-(5-cyano-pyridin-2-yi)-3-(4,7-difluoro-1,1a,2,7b-tetrahydro-cyciopropa[c]chromen-1-yl)-urea (see Example 15) was separated into optically active,, compounds by using a chi.ral AGP 150 x 10 mm, 5jj.m; Crom,. Tech LTD Colomn. The flow rate was set to 4 ml/min. The mobile phase was 89 vol % 10mM HOAc/NhUOAc in acetonitrile. Two elution peaks are seen. The isomer eluting second, typically exhibiting negative rotation is particularly active.
Without in any way wishing to be bound by this observation, it is believed that the more slowly eluting isomer bears the absolute configuration depicted below, which has been established by reference to x-ray crystallographic coordinates of the unsubstituted analogue of Example 1 liganded within reverse transcriptase enzyme. The configuration depicted below is clearly seen in the solved structure, whereas the other enantiomer is not present.
F
(Formula Remove)
>•"»
-N
Example 31
(-) c/s-1 -(5-Chloro-pvridin-2-vn-3-(4,7-difluoro-1,1 a.2.7b-tetrahvdro-
cycloproparclchromen-1 -yD-urea
Racemic (+/-)-c/s-1 -(5-chloropyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea (see Example 17) was separated into optically active compounds by using a chiral AGP 150 x 10 mm, 5jim; Crom Tech LTD Colomn. The flow rate was set to 4 ml/min. The mobile phase was 89 vol % 10mM HOAc/NH4OAc in acetonitrile. Two elution peaks at 27.7 min

and 33.2 min are seen. I he title isomer eiuting at 33.2 min, typicaily exhibiting negative rotation, is particularly active.
Example 32
.(r)c/s-1 -(5-Cv.ano-pyrid?n-2-vlV3-(7-fluQro-4-chloro-1,1 a,2.7b-tetrahvdro-
cycloproparclchrornen-1 -yl)-urea
a) Resolution of the racemic c/s-7-fluoro-4-chloro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
0.32 g (1.32 mmol) of racemic c/s-7-fluoro-4-chloro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid was dissolved in hot acetonitrile (50 ml) and (1R,2R)-2-benzyloxycyclopentylamine (0.25 g, 1.32 mmol) was added. The resulting solution was left for crystallization. After few hours the mother liquor was decanted and crystals were washed with acetonitrile. The second crystallization from acetonitrile gave 92 mg of pure diastereomeric salt. The salt was treated with 1M HCI and resulting mixture was extracted with ethyl acetate. The organic phase was washed with water, brine and evaporated to give 0.05 g of enantiomeric c/s-7-fluoro-4-chloro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
b) (-)c/s-1-(5-Cyano-pyridin-2-yl)-3-(7-fluoro-4-chloro-1l1a,2,7b-tetrahydro-cyclopropa[c]chromen-1-yl)-urea.
The title compound was synthesized analogously to Example 1c) from enantiomeric c/s-7-fluoro-4-chIoro-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid (50 mg). Yield 60.2 mg (84%). [a]D= -0.388 (c=0.5, CHCI3).

Example 33
-r/-c/s-/V-(5-cvano-2-pvridinvl)-A/'-(4,7-dichloro-1.1a.2.7b-
tetrahydrocvclopropal'clchrornen-1-vnurea
a) 1,4-dichioro-2-(2-propynyloxy)benzene
Cl
(Formula Remove)
,0
Cl
2,5-Dichlorophenol (8 g, 49 mmol) was mixed with potassium carbonate (13.6 g, 98 mmol) and 80% solution of propargyl bromide in toluene (11 ml, 98 mmol) in acetone (100 ml) and stirred overnight at room temperature. The precipitate was removed by filtration and washed with acetone. The acetone solution obtaind was concentrated by rotary evaporation and kept under vacuum for 5 h. The product was obtained as yellow oil with quantitative yield. It was used for futher transformations without additional purification.
b) 5,8-dichloro-2H-chromene
Cl
(Formula Remove)

O'
Cl
1,4-Dichloro-2-(2-propynyloxy)benzene was degassed and heated at stirring under argon for 4 h at 224°C. The reaction mixture was then distilled in Kugelrohr apparatus (150-175°C/4.1x10"2mbar) to give 3.58 g of desired product as white solid. Yield 36% from starting dichiorophenol.

c) -f/-c/s-ethyl 4,7-dich!oro-1 Ja,2,7b-tetrahydrocyclopropa[c]chrornene-1-carboxylate
(Formula Remove)
COOEt
0 Cl

5,8-DichIoro-2W-chromene (3.15 g, 16 mmoi), (Rh(l!)Ac2)2 (30 mg, 0.1 mol %) was dissolved in degassed dry methylene chloride (3 ml). Ethyl diazoacetate (3 mi, 2 eq.) in the same solvent was added by a syringe at the flow rate 0.4 ml/h over a period of approximately 5 h under Na atmosphere. The reaction mixture was then washed with NH4CI (aq), water and brine and the solvent removed. The product (45 % cis, 55 % trans) was purified by chromatography on silica (200g, ethyl acetate/n-hexane 1:15) to give 0.9 g of the pure cis product (racemate). Yield 20 %. M*=287.

1H-NMR (CDCI3): 7.15 (d, 1H, J=S.5Hz), 6.91 (d, 1H, J=8.8Hz), 4.59 (dd, 1H, J1=12.02, J2=7.03), 4.48 (dd, 1H, J1=12.02, J2=4.10), 4.07-3.94 (m, 3H), 2.62 (t, 1H, J=8.8Hz), 2.27 (t, 1H, J=8.36Hz), 2.20-2.12 (m, 1H), 1.1 (t, 3H).

d) •4-/-c/s-4,7-dichloro-1,1a,2!7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid
(Formula Remove)
COOH
0
Cl

-r/-c/s-Ethyl4l7-dichloro-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylate was mixed with methanol (3 ml) and water solution of NaOH (1.5 eq., 3 mi) and heated at stirring for 1.5 h at 60°C. The extraction of basic reaction mixture into hexane showed that no starting material present, The reaction mixture was acidified with excess of 3M HCI solution (pH=1). The precipitate formed was collected by suction and washed with water. White solid obtained was dried under high vacuum (yield 80%).

e) +/-c/s-A/-{5-cyano-2-pyridinyl)-A/-{4,7-dichloro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-1-yl)urea
,o
(Formula Remove)
n
M— Cl

+/-c/s-4,7-dichloro-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid

(100mg, 0.39mmol) was mixed with toluene (3ml), triethylamine (1.1 eq), 5-cyano-2-aminopyridine (1.1 eq), DPPA (1.1 eq) and bubbled with argon for about 5 min. The reaction mixture was then heated at stirring at 115°C for 3 h under argon. The reaction mixture was concentrated by rotary evaporation and mixed with small amount of dry ethanol. The precipitate formed was collected by suction and washed with ethanol (2x2 ml) Desired product (+/~cis isomer) was obtained as beige-white powder (65mg, yield 45%).
v

1H-NMR (DMSP-de): 9-83 (s, 1H), 8.34 (d, 1H), 8.03 (dd, 1H), 7.75 (brs, 1H), 7.44 (d, 1H), 7.30 (d, 1H), 7.10 (d, 1H), 4.43 (dd, 1H), 4.18 (dd, 1H), 3.55-3.45 (m, ~1H overlapped with H2O signal), 2.54 (dd, 1H), 2.10-2.02 (m, 1H).
Example 34
+/-c/s-A/-(5-chloro-2-pyridinvl)-A/'-(4,7-dichloro-1.1 a.2.7b-tetrahydrocvcloproparc]chromen-1-yl)urea
(Formula Remove)
M "°
M 1
Cl
Cl

+/-c/s-A/-(5-chloro-2-pyridinyl)-A/-(4,7-dichloro-1,1a,2,7b-tetrahydrocyclopropa [c]chromen-1-yl)urea was synthesized analogously to Example 33 from +/-CAS-4,7-dichlorc-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylicacid

(100 mg, 0.39mmol) and 2-amino-5-chloropyridine (1.1 eq) to give 66 mg of product as white powder. Yield 44%.
1H-NMR (DMSO-de): 9.47(s, 1H), 7.98 (d, 1H), 7.86 (brs, ~1H), 7.83 (dd, 1H), 7.30 (d, 1H), 7.23 (d, 1H), 7.10 (d, 1H), 4.44 (dd, 1H), 4.18 (dd, 1H), 3.55-3.48 (m, 1H), 2.54 (dd, 1H), 2.10-2.02 (m, 1H).
Example 35
+/-c/s-A/-(5-bromo-2-pyridinvl)-/V-(4,7-dichloro-1,1 a,2,7b-tetrahvdrocvclopropafclchromen-1-vl)urea
H //°
(Formula Remove)


+/-c/s-/V-(5-bromo-2-pyridinyl)-A/'-(4,7-dichloro-1,1 a,2,7b-tetrahydrocyclopropa [c]chromen-1-yl)urea was synthesized analogously to Example 33 from +/-CYS-4,7-dichloro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (100 mg, 0.39mmol) and 2-amino-5-bromopyridine (t.1 eq) to give 35 mg of product as grey powder. Yield 21%.

1H-NMR(DMSO-d6):9.47(s, 1H), 7.97 (d, 1H), 7.86 (br s, ~1H), 7.83 (dd, 1H), 7.30 (d, 1H), 7.23 (d, 1H), 7.10 (d, 1H), 4.43 (dd, 1H), 4.18 (dd, 1H), 3.55-3.48 (m, 1H), 2.54 (dd, ~1H overlapped with DMSO signal), 2.08-2.01 (m, 1H).
Example 36
+/-c/s-A/-(5-phenoxv-2-pvridinvi)-A/-(4.7-dichloro-1,1a.2.7b-
tetrahvdrocvcloproDafc]chromen-1-yl)urea
a^°
C!
(Formula Remove)

+/-c/s-A/-(5-phenoxy-2-pyridinyl)-A/-(4,7-dichloro-1,1a,2,7b-tetrahydrocyclopropa [c]chromen-1-yl)urea was synthesized analogously to Example 33 from 4-/-as-4,7-dichloro-1,1a,2,7b-
tetrahydrocyclopropa[c]chromene-1-carboxylic acid (58 mg, 0.22 mmol) and 2-amino-5-phenoxypyridine (1.1 eq) to give 49 mg of product as slightly brownish powder. Yield 49%.
1H-NMR (CDCI3): 9.30 (brs, 1H), 8.26 (s, 1H), 7.53 (d, 1H), 7.35 (m, 2H), 7.25 (dd, 1H), 7.16-7.10 (dd, ~1H overlapped with CHCI3 signal), 7.05 (d, 1H), 6.97-6.90 (m, 3H), 6.72 (d, 1H), 4.46 (dd, 1H), 4.30 (dd, 1H), 2.73 (m, 1H), 2.63 (dd, 1 H), 2.05-1.95 (m, 1 H).
Example 37
+/-c/s-A/-f7-chloro-4-fluoro-1,1a,2.7b-tetrahvdrocyclopropafdchromen-1-vl)-
A/-f5-cvano-2-pyridinyl)urea .. •
a) 5-chloro-2-fluorophenol
(Formula Remove)

5-Chloro-2-fiuoroaniline (10g, 68 mmol) was dissolved in 6M sulfuric acid and cooled in ice/brine bath to -5°C. The solution of NaNO2 (5.2 g, 76 mmol) in minimum amount of water was added dropwise to the stirred suspension at the temperature not higher then -2°C. After the addition clear yellow solution formed was allowed to stir for additional 30 min at cooling. CuSCU was
dissolved water (80 ml) and mixed with sulfuric acid (32 ml). The diazonium salt solution was added dropwise to the preheated (160°C) cuprous sulfate solution and the product was removed from the reaction flask by steam distillation. The reaction took about 2 h to be complete. The water/phnol solution was extracted into ether, washed with brine and dried over I Concentration gave 4g of crude phenol (40%).
b) 4-chloro-1 -fluoro-2-(2-propynyloxy)benzene
F
,CK
(Formula Remove)

ci
M3h!oro-1-fluoro-2-(2-propynyloxy)benzene was synthesized analogously to Example 33a from (4 g, 27 mmol) 4-chloro-1-fluorophenol to give 4.6g of iroduct (purified by column chromatography on silica, ethyl acetate/n-hexane :15) as yellow oil. Yield Q0%.
) 5-chloro-8-fluoro-2H-chromene
CI
(Formula Remove)

o
•Chloro-8-fluoro-2H-chromene was synthesized analogously to Example o3b) from 4-chloro-1-f!uoro-2-(2-propynyloxy)benzene (4.6 g, 25 mmol) to give 1 g of product (purified by column chromatography on alumina, ethyl acetate/n-hexane 1:15) as colourless oil. Yield 22%.
d) ethyl +/-c/s-7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocycloprcpa[c]chromene-1-carboxylate
COOEf
(Formula Remove)

F
Ethyl +/-c/s-7-ch!oro-4-fIuoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromene-1 -carboxylate was synthesized analogously to Example 33c from 5-chloro-8-fluoro-2f/-chromene (1 g, 5.4 mmol) to give 360 mg of +/-cis product (purified by column chromatography on silica, ethyl acetate/n-hexane 1:20) as white solid. Yield 25%.
e) -f/-c/s~7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocyclopropa[c|chromene-1 -carboxylic acid
P. COOH
v' -"
(Formula Remove)

+/-c/s-7-Ch Joro-4-fluoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromene-1 -carboxylic acid was synthesized analogously to Example 33d from ethyl +/-c/s-7-chIoro-4-fluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylate (360 mg, 1.3 mmol) to give 259 mg of +/-CAS acid (80%).
f) +/-c/s-A/-(7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromen-1-y!)-/V-(5-cyano-2-pyridinyl)urea
,O
(Formula Remove)
N=+/-c/s-A/-(7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocyciopropa[c]chromen-1 -yl)-/V-(5-cyano-2-pyridinyl)urea was synthesized analogously to Example 33e from
-f/-c/s-7-chloro-4-fluoro-1,1a;2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (60 mg, 0.25mmol) and 2-amino-5-chIoropyridine (1.1 eq) to give 59 mg of product as white powder. Yield 66%.
'H-NMR (DMSO-d6): 9.47 (brs, 1H), 7.89 (d, 1H), 7.80 (brs, 1H), 7.74 (dd, 1H), 7.32 (d, 1H), 7.16-7.05 (m, 2H), 4.39 (dd, 1H), 4.16 (dd, 1H), 3.55-3.48 m, 1H), 2.51 .(.dd, ~1H overlapped with DMSO signal), 2.08-2.01 (mJH).
Example 38
•/-c/s-/V-(7-chloro-4-fluoro-1,1a,2,7b-tetrahvdrocvcloproparc1chromen-1-vl)-A/-
5-chloro-2-pvridinvl)urea
(Formula Remove)

O F
f/-c/s-A/-(7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromen-1 -yl)-/V-(5-chloro-2-pyridinyl)urea was synthesized analogously to Example 5 from +/-c/s-7-chloro-4-fluoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromene-1 -carboxylic acid (60 mg, 0.25mmol) and 2-amino-5-chloropyridine (1.1 eq) to give 59 mg of product as white powder. Yield 65%.
1H-NMR (DMSO-d6): 9.47 (br s, 1 H), 7.89 (d, 1 H), 7.80 (br s, 1 H), 7.74 (dd, 1H), 7.32 (d, 1H), 7.16-7.04 (m, 2H), 4.39 (dd, 1H), 4.16 (dd, 1H), 3.55-3.48 (m, 1H), 2.51 (dd, ~1H overlapped with DMSO signal), 2.06-2.01 (m, 1H).
-/-c/s-A/-(5-bromo-2-DVridinvl)-/V-f7-chlqro-4-fluoro-1.1a,.2.7b-tetrahydrocvcloproparclcnrornen-1-vflurea
(Formula Remove)
O
O F

+/-c/s-A/-(5-brorno-2-pyridinyl)-A/-(7-chloro-4-fluoro-1!1a,2,7b-tetrahydrocyclopropa [c]chromen-1-yl)urea was synthesized analogously to Example 32e from +/-c/s-7-chloro-4-f!uoro-1,1a,2,7b-
tetrahydrocyclopropa[c]chromene-1-carboxylic acid (60 rng, 0.25mmol) and 2-amino-5-bromopyridine (1.1 eq) to give 56 mg of product as white powder. Yield 55%.

'H-NMR (DMSO-d6): 9.46 (brs, 1H), 7.96 (d, 1H), 7.83 (dd, 1H), 7.81 (brs, 1 H), 7,27 (d, 1 H), 7.16-7.04 (m, 2H), 4.38 (dd, 1 H), 4.17 (dd, 1 H), 3.55-3.48 (m, 1H), 2.51 (dd, -1H overlapped with DMSO signal), 2.07-2.00 (m, 1H).
15
Example 40
+/-c/s-/V-(7-chloro-4-fluoro-1,1a,2.7b-tetrahvdrocvcloproparc1chromen-1-vn-/V-
f5-phenoxv-2-pvridinyl)urea
(Formula Remove)

0 F

4-/-c/s-jV-(7-Chloro-4-fluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-1-yl)-/V-(5-phenoxy-2-pyridinyi)urea was synthesized analogously to Example 32e from +/-c/5-7-chloro-4-fluoro-1,1a!2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (60 mg, 0.25 mmol) and 2-amino-5-phenoxypyridine (1.1 eq) to give 76 mg of product as slightly brownish powder. Yield 73%.

'H-NMR (CDCI3): 9.33 (br s, 1H), 7.93 (s, 1H), 7.51 (d, 1H), 7.38-7.32 (m, 2H), 7.25 (dd, ~1H overlapped with CHC(3 signal), 7.16-7.10 (m, 1H), 6.96-6.88 (m, 3H), 6.79 (dd, 1H), 6.68 (d, 1H), 4.45 (dd, 1H), 4.25 (dd, 1H), 3.75-3.70 (m, 1H), 2.61 (dd, 1H), 2.05-1.95 (m, 1H).
Example 41
/V-r(1S. 1aR. 7bR) or MR. 1aS. 7bSV1.1a. 2. 7b-tetrahydrocvc!oproparc]-
[11benzothiopyran-1-vri-/V'-(5-cvano-2-pvridinvl) urea
a) 3,4-dihydro-2f/-1 -benzothiopyra (Formula Remove)
n-4-ol



A solution of thiochroman-4-one (9g) in ether (27 ml) was added slowly to a mixture of lithium aluminium hydride (0.53 g) in ether (54 ml). After the end of the addition, the mixture was refluxed for 2 hours. The reaction mixture was cooled and ice was added, followed by water and by a solution of 20% H2SC>4. The water phase was washed twice with ether. The ether phase was '
washed twice with NaOH 2N, and once with water, dried over MgSC>4 and evaporated. The clear oil (8.9 g) crystallised after few hours. Rdt = 97%


b) 2H-1-benzothiopyran and 4H-1-benzothiopyran ,H
(Formula Remove)

4-Thiochromanol (8.9 g) and potassium acid sulfate (0.89 g) were placed in a
flask and evacuated to 1 mm. The flask was put in a bath heated at 90 °C until the alcohol melted. The magnetic stirrer was started and the bath slowly brought to 120 °C. Dehydration was rapid and a mixture of the product and water distilled and was collected in a ice-cooled receiver. The product was

taken up in ether and dried. The crude product (7g, Rdt= 88%) wasn't purmti. The NMR showed the presence of 10% of the 4H-1~benzothiopyran.
c) Ethyl ester 1, 1a, 2, 7b-tetrahydro~cyclopropa[c][1]benzothiopyran-1-carboxyiic acid, (1S, 1aR, 7bR) or(1R, 1aS, 7bS)
(Formula Remove)






Ethyl diazoacetate was added slowly to 500 mg of thiochromene at 140 C. The reaction was followed,by Gas chromatography and stopped when all starting material was consumed (about 7 hours). The residue was purified by flash chromatography (5% ether in hexane). The cis isomer (46,5 rng, Rdt = 6%) was identified by NMR spectroscopy.
d) 1, 1a, 2, 7b, tetrahydrc-cyclopropa[c][13benzothiopyran-1-carboxylic acid, (1S, 1aR, 7bR) or(1R, 1aS, 7bS)
(Formula Remove)



A mixture of the cis isomer (46,5 mg), LiOH (4eq., 19 mg) in 5 m! of methanol / 25% H£O was refiuxed for 1 hour. After evaporation of the solvent under
vacuum, the residue was dissolved in water and washed with ether. The wat
s) A/-[(1S, 1aR, 7bR) or(1R, 1aS, 7bS)-1. 1a,2,7b-tetrahydrocyclopropa[c][1]benzothiopyran-1-yl]-/V-(5-cyano-2-pyridinyl) urea


(Formula Remove)
The cis acid (30 mg) was refluxed for 4 hours in toluene (2 ml) in presence of EtsN (0.02 ml), diphenyl phosphonic azide (0.03 ml) and 2-amino 6-cyanopyridine (19,5 mg). After cooling, the toluene phase was washed with water, followed by a solution of HCI (0.01 M). The organic phase was dried and evaporated. The residue was purified by flash chromatography (EtOAc 2 / Hexane 1) and gave 10 mg of the desired compound. Rdt = 22%.
1H (DMSO-d6): 1.96 (1 H, m); 2.30 (1 H, t, 8.6); 2.71 (1 H, ddt, 13.65, 6.24);
3.24 (2H, m); 7.19 (3H, m); 7.37(1 H, dd, 7.4, 1.56); 7.42 (1H, dd, 9.0, 3.1); 7.60 (1H, NH); 8.02 (1H, dd, 9.0, 2.3); 8.15 (1H, s); 9.89 (1H..NH)
Mass: 322 (M+-), 321 (M-H)
Example 42
(1 S, 1 aR7bS)-4,7-dif luoro-1,1 a.2 Jb-tetrahydrocvclopi'opal'clchrornene-1 -
carboxylic acid

a) (2Z)-3-(3,6-difluoro-2-methoxyphenyl)-2-propen-1-ol. F

O'
(Formula Remove)
1)BuLi/ZnCI2;THF 2) Pd(OAc),;
Br
COOEt
3) DIBAL
Mw=144
Mw=200
A solution of BuLi (2.5M) in hexane (9.6 ml; 0.024 mol) was added to a stirred solution of 2,5-difluoroanisol (2.88 g, 0.02 mol) in dry THF (30 ml) at-70C, followed after 2h by solution of zinc chloride (3.6 g ; 0,026 mol) in dry THF (50

ml). The reaction temperature was allowed to raise to room temperature and then stirring was maintained at room temperature for 30 min. Pd(OAc)a (8 mg; 0,2 mol %) was added, followed by ethyl cis-3-bromoacryIate (3.58 g ; 0.02 mol). The reaction mixture was placed in preheated oil bath and heated under reflux for 1h. The resulting reaction mixture was chilled to -78 C and 60 ml (0.06 mol) of DIBAL (1M solution in hexanes) was added dropwise. The stirring was continued at -78 C.for 2h and 1h at room temperature. The reaction was quenched with water and all solids were dissolved by addition of HCI. The organic phase was diluted with ether, separated, washed with 5N HCI, brine and evaporated in vacua. The residue was Kugelrohr distilled (1.5x10"2 mbar, 150 C) to give 3.7 g (92%) of crude (2Z)-3-(3,6-difluoro-2-methoxyphenyl)-2-propen-1-ol, which contains -6 % of other regioisomers. The crude product was used in the next step without further purification. 1H-NMR (CDCIa): 7.00 (m, 1H); 6.77 (rn, 1H); 6.31 (app. d, 1H); 6.12 (app. dt, 1H); 4.08 (br. t, 2H); 3.89 (d, 3H); 1.80 (br. t, 1H).
b) (2Z)-3-(3,6~difluoro-2-methoxyphenyl)prop-2enyl diazoacetate


(Formula Remove)
TsNHN=CHCOCI
PhNMe2; NEt3; CH2CI2
v_/
F

° 80%

Mw=200 Mw=268
The p-toluenesulfonyihydrozone of glyoxylic acid chloride (5.16 g; 0.02 mol) was added to a solution of (2Z)-3-(3,6-difiuoro-2-methoxyphenyl)-2-propen-1-ol (3.6 g; 0.01 S mol) in dry CH2Cl2 (50 ml) at -5C, and N,N-dimethylaniIine (2.5 ml; 0.02 mol) was added slowly. After stirring for 30 min at-50, EtgN (12 ml; 0.09 mol) was added slowly. The resulting mixture was stirred for 15 min at 5C and then for 30 min at room temperature, whereupon water (-50 ml) was added. The organic phase was separated washed with water, brine and concentrated in vacuo. Flash chromatography (silica, EA:Hex; 1:15) gave 3.86 g (80 %) of product as a yellow solid.

1H-NMR (CDCI3): 7.00 (m, 1H); 6.76 (m, 1H); 6.41 (app. d, J=12.2 Hz; 1H); 6.00 (app. dt, J=12.2; 6.10 Hz; 1H); 4.71 (br. s, 1H); 4.67 (dt, 2H); 3.89 (d, 3H).
c) (13,5/7,65)- 6-(3,6-difluoro-2-methoxyphenyl)-3-oxabicyclo[3.1.0]hexan-2-one.


(Formula Remove)
Mw=268

Rh2(5-R-MEPY)4x
abs. degassed dichloromethane

Mw=240

(2Z)-3-(3,6-difluoro-2-methoxyphenyl)prop-2enyl diazoacetate (3.45 g, 0.013 mol) was dissolved in 100 ml of dried degassed dichloromethane and added dropwise to the solution of chiral Doyle catalyst (Aldrich, also available from Jonnsson Matthey, 10 mg, 0.1 mol%) in 50 ml of dichloromethane under argon at ambient temperature over a period of ~6 h. The initial blue color had turned to olive by the end of the addition. The reaction mixture was concentrated in vacua and the crude product was purified by flash chromatography (silica, EA:Hex, 1:5-»1:1) to give 2.72 g (88 %) of (1S,5£?,6S)- 6-(3,6-difIuoro-2-methoxyphenyl)-3-oxabicyclo[3.1.0]hexan-2-one as colorless solid. Enantiomeric purity could be checked on this stage using " Chiracel OD column, 10% IPA in hexane - 94% ee.
1H-NMR(CDCI3):7.00(m, 1H);6.72(m, 1H);4.33 (dd, 1H);4.10(d, 1H);4.02 (d, 3H); 2.66 (m, 2H); 2.37 (t, 1H).
d) (1S, 1 afl,7bS)-1-(bromometriyl)-4I7-difIuoro-1 a,7b-dihydrocyc!opropa[c]chromene-2(1H)-one.



30 % HBr/AcOH (Formula Remove)

,. Mw=
Mw=289
(1 S,5R,6S)- 6-(3,6-difluoro-2-methoxyphenyl)-3-oxabicyclo[3.1 .Ojhexan-2-one (130 mg, 0.55 mmol) was mixed with 1.2 ml of 30% HBr/AcOH (6 mmol) and heated in a sealed vessel at stirring for about 4h at 90°C. The reaction mixture was then cooled down, mixed with water and extracted into diethyl ether (3x20 ml). Ether extract was washed with sat. sodium bicarbonate solution and brine. Dried over magnesium sulfate. Concentration gave 160 mg of white solid material. 98% yield.
1H-NMR (CDCI3): 7,08 (m, 1H); 6.88 (m, 1H); 3.44 (dd, 1H); 3.06 (t, 1H); 2.96 {dd, 1H); 2.64 (dd, 1H); 2.46 (m, 1H).

e) (1S,1a/:?,7bS)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]ehromene-1-carboxylic acid. (Formula Remove)


(Formula Remove)
0,
NaOH
H2O

Mw=289

Mw=226

(1S,1a/7,7bS)-1-(bromomethyl)-4,7-difluoro-1a,7b-dihydrocyclopropa [c]chromen-2(1/Ti)-one (360mg, 1.2 mmol) was mixed with the solution of NaOH (0.1 g, 2.5 mmol) in 5 m! of water and heated at stirring for 1h at 90°C. After completion the reaction mixture was cooled down and extracted into diethyl ether (2x20 ml). Water phase was acidified with cone. HCl. The precipitate formed was collected by filtration to give 180 mg of pure product. Mother liquor was extracted into ether and washed with brine, dried over
magnesium sulfate. Concentration gave additional 70 mg of product (containing up to 15% of impurities). Overall yield about 92%. 1H-NMR (CDCI3): 6.86 (m, 1H); 6.54 (m, 1H); 4.48 (m, 2H); 2.62 (t, 1H); 2.20 (t, 1H);2.11(m, 1H).
Example 43 '-) cis A/-f1a, 6/>dihvdro-1/-/-benzo|'b]cvclopropard]thien-1-vn-/V-(5-cvano-2-
pyridinvD-urea




a) cis ethyl ester 1 a, 66-dihydro-1 H-benzo[b]cyclopropa[d]thiophene-1 • carboxylic.acid, (1S, 1aS, 6bR) or(1R, 1aR, 6bS)


(Formula Remove)


Ethyl diazoacetate is added slowly to 10 g of thiophene at 140 °C. The reaction was checked by gas chromatography and stopped after 7 hours. The residue is purified by flash chromatography (5% ether in hexane). The cis isomer (917 mg, Rdt = 6%) was identified by NMR spectroscopy.
Reference: Badger G.M. et al, J. Chem. Soc., 1958, 1179-1184. Badger G.M.et a!, J. Chem. Soc., 1958, 4777-4779.
b) cis 1 a, 6b-dihydro-1 H-benzo[b]cyclopropa[d]thiophene-1 -carboxylic acid, (1S, 1aS, 66R) or(1R, 1aR, 6bS)



C02H
(Formula Remove)
C02Et
A mixture of the cis isomer (443 mg), LiOH (193 mg) in 15 ml of methanol / 25% H£O is refluxed for 1 hour. After evaporation of the solvent under
vacuum, the residue is dissolved in water and washed with ether. The water phase is acidified with concentrated HCI, and extracted twice with
aicnicrometnane. Aner drying, the organic phase is evaporated and gave the desi'red acid (313.6 mg). Rdt = 81%.
c) (+/-) cis N-[\ a, 6/xJihydro-l /-/-benzo[b]cyciopropa[d]fhien-1 -yl]-A/-(5-cyano-2-pyridinyl)-urea


(Formula Remove)

C02H
The cis acid (313 mg) was refiuxed for 4 hours in toluene (20 ml) in presence of EtsN (0.25 ml), diphenyl phosphoryl azide (0.3 mi) and 2-amino 6-
cyanopyridine (220 mg). After cooling, the toluene phase was washed with water, followed by a solution of HCI (0.01 M). The organic phase was dried and evaporated. The residue was purified by flash chromatography (EtOAc 2 / Hexane 1) and gave 10 mg of the desired compound. Rdt = 2%.
1H (DMSO-d6): 3.32 (1H, m); 3.39 (1H, td, 8.05, 7.69); 3.52 (1H, dd, 7.69, 6.22); 7.08 (1H, td, 7.32, 1.1); 7.15 (1H, td, 7.32, 1.1); 7.22 (1H, dd, 8.4, 0.8); 7.39 (2H, m); 7.50 (1H, NH); 8.00 (1H, dd, 8.79, 2.2); 8.23 (1H, d, 2.2); 9.76 (1H, NH)
13c (DMSO-d6): 25.6 (CH), 29.5 (CH), 33.7 (CH), 101.5 (C), 112.1 (CH), 118.0 (C), 122.1 (CH), 124.9 (CH), 127.3 (CH), 128.0 (CH), 136.3 (C), 141.7 (CH), 143.7 (C), 151.6 (CH), 155.1 (C), 156.1 (C) Mass: 310 (M+2), 309 (M+H)
Example 44
C-)-c/5-1-(5-Chloro-pvridin-2-vl)-3-(4,7-difluoro-1.1a.2.7b-
tetrahydrocvclopropafcIchromen-l-vO-urea
(Formula Remove)his compound was prepared analogously to Example 1c but using chiral (+)-c/s-4,7-difluoro-1,1 a,2,7b-tetrahydrocyclopropa[c]chromen-1-carboxylic acid (see Example 42e) (1.3 g, 5.75 mmol). The silica gel purified product was recrystallized from acetonitrile to give 0.95 g (47%) of the title product. Absolute stereochemical configuration assigned as for Example 30.
1H-NMR (CDCI3): 9.25 (broad s, 1H), 8.67 (s, 1H), 7.79 (d,,1H), 7.48 (dd, 1H), 6.92-6.86 (m, 1 H), 6.71 (d, 1 H), 6.65-6.60 (m, 1 H), 4.45 (dd, 1 H), 4.34 (dd, 1 H), 3.80 (q, 1 H), 2.61 (t, 1 H), 2.00-1.98 (m, 1 H).
Example 45
f-)-c/s-1-(5-Cvano-pvridin-2-vn-3-(4.7-difluoro-1,1a.2.7b-tetrahvdro-
cvcloproparc1chromen-1-vl)-urea

HN
(Formula Remove)
O
F
f+,)-c/s-4,7-Difluoro-1,1 a,2,7b-tetrahydro-cycloprQpa[c]chromene-1 -carboxylic acid (see example 42e) (1,18 g, 5.2 mmol), diphenylphosphorylazide [1340 JJ.L, 6.3 mmol (d=1.277)], triethylamine (870 pL, 6.3 mmol) and 2-amino-5-cyanopyridine (740 mg, 6.3 mmol) were dissolved in toluene (15 mL) and refluxed for 4 h. The solvent was then removed in vacuo and the crude product was dissolved in ether and washed (3 x 100 mL 0.01 M HCI) and purified by chromatography (silica gel, 0-^1% MeOH in ether) to give pure (-)-c/s-1 -(5-cyano-pyridin-2-yl)-3-(4,7-difluoro-1,1 a,2,7b-tetrahydro-cyc!opropa[c]chromen-1-yl)-urea (1.1 g, 64%). ee 92% as determined by HPLC on a Chiral AGP column, eluent 11 % acetonitrile in sodium phosphate buffer, flow 0.9 mLymin. Absolute stereochemical configuration assigned as for Example 30.
'H-NMR (CDC!3): 9 (s, NH), 8.42 (s, NH), 8.16 (d, 1H), 7.72 (dd, 1H), 6.97-6.76 (m, 2H), 6.69-6.61 (m, 1H), 4.47 (dd, 1H), 4.31 (dd, 1H), 3.75 (m, 1H), 2.65 (t, 1H), 2.05-1.96 (m, 1H).
Example 46
f-)-c/5-1-(5-cyano-pyridin-2-vl)-3-f4,7-difluoro-1.1a.2,7b-tetrahvdro-
cvcloproparc]chromen-1-yl)-thiourea
(Formula Remove)
,S
N=EN
(•fJ-c/s-4,7-Difluoro-t, 1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid (2.2 g, 9.7 mmol), DPPA [2380 jxl, 10.7 mmol 97%( d=1.277)] and TEA (1510 jil, 11.7 mmol) was refluxed in toluene (20ml) for 2 h. Dioxane (26 mL) and HCI(aq) (26 ml, 6M) was then added and the reaction mixture was left for 1 -2 h. At 50 °C. Water (50 ml) was added and the water phase was washed with Ether (2 x 25 ml) and then made alkaline with ammonia (aqj. Extraction with dichloromethane and drying gave the intermediate 4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromen-1y!amine (1.37 g, 71%), which was directly treated with 6-isothiocyanato-nicotinonitrile (1.25 g, 7.7 mmol) in acetonitrile (2 mL) at RT over the weekend. The precipitated crystals were filtrated off and the solvent removed in vacuo and chromatographed (silica, 20 % ether in pentane). The product obtained was combined with the crystals and the crude product (900 mg) was re-crystallised (ethanol-acetone) to give pure (-)-c/s-1-(5-cyano-pyridin-2-yl)-3-(4,7-dif!uoro-1,1 a,2,7b-tetrahydro-cyclopropa[c]-chromen~1-yl)-thiourea (590 mg 18%). Absolute stereochemical configuration assigned as for Example 30.
1H-NMR (CDCI3-MeOD): 8.1 (d, 1H), 7.77 (dd, 1H), 6.99-6.91 (m, 1H), 6.74 (dd, 1 H) 6,73-6.66 (m, 1 H), 4.48 (dd, 1 H), 4.33 (dd, 1 H), 4.20( dd, 1 H), 2.78(t, 1H), 2.16-2.1(m, 1H).
Example 47
(±)-c/s-1 -(5-brornoDvridin-2-vn-3-(7-fluoro-4-propionyl-1.1 a,2.7b-tetranydro-
cvcloDroparclchromen-1-ylHirea.
(Formula Remove)

F
a) 1 -(4-F!uoro-2-prop-2-ynyloxy-phenyl)-propan-1 -one. To a mixture of NaH (95%, 278 mg, 1 1 mmol) in DMF (20 ml) at 0 °C, was added 1-(4-fluoro-2-hydroxy-phenyl)-propan-1-one (1.68 g, 10 mrriol) in DMF (5 mL). After 15 min at 0 °C, was 3-bromo-propyne (3.02 g, 20 mmol) added to the reaction mixture. After 1h at 0 °C, was the reaction mixture allowed to assume room temperature. The reaction mixture was extracted with H2O (100 mL). The H2O phase was washed with Et2O (3x100 mL) and the solvent of the combined organic phases was removed under reduced pressure. The crude product was purified by column chromatography (silica gel, CH2Cl2), to give 1.40 g (68%) of 1-(4-fluoro-2-prop-2-ynyloxy-phenyl)-propan-1-one.
(CDCI3): 7.64 (dd, 1H), 6.69 (dd, 1H), 6.60 (ddd, 1H), 4.68 (d, 2H), 2.85 (q, 2H), 2.58 (t, 1H), 1.03 (t, 3H).
b) 1 -(5-Fluoro-2/-/-chromen-S-yl)-propan-1 -one.
1-(5-Fluo.ro-2H-chromen-8-yl)-propan-1-one was synthesized analagously to Example 3b from 1-(4-fluoro-2-prop-2-ynyloxy-phenyl)-propan-1-one (1.34 g, 6.5 mmol), to give 619 mg (46%) of 1-(5-fluoro-2H-chromen-8-yl)-propan-1-one.
1H-NMR (CDCI3): 7.60 (dd, 1H), 6.67-6.58 (m, 2H), 5.86 (dt, 1H), 4.76 (dd, 2H), 2.93 (q,2H), 1.23 (t,3H).
c) (±)-c/s-7-Fluoro-4-propiony!-1 ,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid ethyl ester.
(±)-c/s-7-Fluoro-4-propionyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-
carboxylic acid ethyl ester was synthesized according to method 3c) from 1-
(5-fiuoro-2/-/-chromen-8-yl)-propan-1-one (619 mg, 3 mmol), to give 142 mg
(16%) of (±)-c,fe-7-f!uoro-4-propionyi-1,1a,2,7b-tetrahydro-
cyc!opropa[c]chromene-1-carboxylic acid ethyl ester and (±)-frans-7-f!uoro-4-
propionyI-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl
ester as-a byproduct. . •-••..
1H-NMR (CDCI3): 7.59 (dd, 1H), 6.65 (m, 1H), 4.50-4.46 (m, 2H), 3.95 (q, 2H); 2.89 (q, 2H), 2.57 (dd, 1H), 2.20 (dd, 1H), 1.13-1.03 (m, 1H), 1.12-1.01 (m, 6H).
d) (±)-c/s-7-Fiuoro-4-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid.
(±)-c/s-7-Fluoro-4-propionyl-1,1a,2,7b-tetrahydro-cyclopropa[c]chromene-1-carboxylic acid was synthesized analogously to Example 1 b from (±)-c/s-7-fluoro-4-propionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid ethyl ester (140.3 mg, 0.48 mmol), to give 83 mg (65%) of (±)-c/s-7-fluoro-4-prbpionyl-1,1 a,2,7b-tetrahydro-cyclopropa[c]chromene-1 -carboxylic acid as a white solid. The crude product was purified by column chromatography (silica gel, 1-»5% MeOH in CH2Cl2).
1H-NMR (DMSO-de): 12.15 (brs, 1H), 7.46 (dd, 1H), 6.78 (dd, 1H), 4.57 (dd, 1H), 4.43 (dd, 1H), 2.93-2.80 (m, 2H), 2.55 (dd, 1H),2.24(dd, 1H), 2.20-2.10 (m, 1H), 1.02(t,3H).
e) (±)-c/s-1 -(5-bromopyridin-2-yl)-3-(7-fluoro-4-propionyl-1,1 a,2,7b-
tetrahydro-cyclopropa[c]chromen-1-yl)-urea.
The title compound is synthesized analogously to example 1c) by reacting 1
equivalent of (±)-os-7-flucro-4-propionyl-1,1 a,2,7b-tetrahydro-
cyclopropa[c]chromene-1-carboxylic acid and 1 eq of triethylamine in toluene
with 1 eq of diphenylphosphoryl azide for 30 minutes at room temperature.
The reaction mixture is heated to 120°C and an approximately equimoiar
solution of 2-amino-5-bromopyridine is added. After 3 hours the solution is allowed to assume room temperature and the title compound extracted as shown above.
Example 48
(1S,5R,6S)-6-(3,6-difiuoro-2-methoxyphenyl)-2-methoxy-3-oxabicyclo[3.1.Ojhexane

(Formula Remove)
OMe F

a) lodo-3-oxabicyclo[3.1 .0.]hexan-2-one

0
A
,.i - l-llll
The title compound is synthesised in the depicted stereochemistry as described in Doyle J Amer Chem Soc 117 (21) 5763-5775 (1993)
b) Iodo-2-methoxy-3-oxabicyclo[3,1 .Ojhexane
9v HO MeO
. . -yv-Q- . DIBAL ^-Q' -MeOH, H+ ^—Q
, A / —- ^A I A I
The title compound is synthesised in the depicted stereochemistry as described in Martin et al Tett Lett 39 1521-1524 (1998),
c) (1 S,5R,6S)-6-(3,6-difluoro-2-methoxyphenyl)-2-methoxy-3-oxabicyclo[3.1.0]hexane



Me°v 1)BuLi,ZnCI2
>-n 2) Pd(OAc)2/'Ligand
OMe r THF
F (Formula Remove)
F
2,4-diflouroanisol (90 mg, 0.62 mmol) was dissolved in anhydrous, degassed,
THF (7 ml) and cooled to -78 °C under N2. nBuLi, 2,5 M in hexane, (0.30 ml, 0.77 mmol) was added and the reaction mixture was stirred at -78 °C for 2 hrs. 2nCI2 (150 mg, 1.1 mmol), as a solution in anhydrous THF (7 ml), was added and the reaction mixture was allowed to warm to ambient temperature for 2 hrs. lodo-2-methoxy-3-oxabicyc!ohexane (150 mg, 0.63 mmol), Pd (OAc)2 (1.5 mg, 6.2 u.mol), and ligand Tris(2,4-di-te/t-butylphenyl)phosphite (40 mg, 62 jjmol) were mixed in anhydrous THF (7 ml) and added to the reaction mixture. The reaction mixture was heated at reflux for 3 days and quenched with HgO. Diethyl ether was added and the layers were separated, the organic layer was washed with H2O and aq. sat. NaCI, dried over MgSO^, filtered and concentrated to give the title compound, otherwise denoted 2,4-di-fluoro-5-(cyclopropylacetal)anisol. Column chromatography on silica (EtOAc/Hexane 1:3) gave (4) 50 mg, 31%.
1H NMR (CDCI3) 5 (ppm): 6.88-6.94 (m, 1H, ArH), 6.68-6.73 (m, 1H, ArH), 4.82 (s, 1H, CA/OCHg), 3.97-3.98 (m, 1H, CHOCH) 3.94 (s, 3H, OCH3), 3.79-3.81 (m, 1H, CHOCH) 3.30 (s, 3H, OCW3), 2.13-2.19 (m, 2H, 2x CH-cyclopropyl), 1.89 (tr, ^7.81 Hz, 1H, CHcyclopropyl).
Example 49
c/s-4,7-Difluoro-1. 1 a,2.7b-tetrahvdro-cycloDroparc]chromene-1 -carboxvlic acid.
(Formula Remove)

>C02H
F
BBr31M solution in CH2CI2 (5.8 ml; 5.8 mmol 2.1 eq) was added to starting lactone, (15,5/7,6$)- 6-(3,6-difluoro-2-methoxyphenyl)-3-oxabicyclo[3.1.0]hexan-2-one from example 42c) (0.66 g; 2.75 mmol) at 0°C. The reaction mixture was stirred at 0°C for 1h. Acetonitrile (5.8 ml) was added and stirring was continued for 3h at 0°C. The reaction mixture was quenched by addition of water and the organic phase was separated. Water phase was extracted with CH2CI2 and combined organic phases were evaporated: NaOH (0.33 g; 8.25 mmol; 3 eq) in water (~5 ml) was added to the resulted residue and stirred at 80 °C for 45 min. The reaction mixture was extracted with ether to remove none acidic impurities. The residual ether in water phase was evaporated in vacuo and cone. HCI was added to pH of ~3. After ~1h the solid was filtered off yielding 0.497 g (80%) of crude final acid as brownish solid. The crude acid was dissolved in 6 ml of EtOH/H2O (40/60 v/v) and treated with activated carbon. The hot solution was filtered and left for crystallization. Yield 0.4 g (64%).
Example 50
A/-r(lS,1afi',7b/:?)-4.7-difluoro-1,1a;2.7b-tetrahvdrocvcloproparclchromen-1-vn-
/V-(5-flupro-2-pvridinvl)urea
(Formula Remove)

(1S,1af?,7bS)-4,7-difluoro-1J1a,2,7b-tetrahydrocycIopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmol, ee -90%) was mixed with toluene (1ml), triethylamine (0.034 ml, 1.1 eq), 2-amino-5-fluoropyridine (28 mg, 1.1 eq), DPPA (0.054 mi, 1.1 eq). The reaction mixture was then heated at stirring at
110°C for 3 h. The reaction mixture was concentrated by rotary evaporation and purified by column chromatography on silica (50g, ethyiacetate/hexane 1:1) to give 30 mg of the product as white solid.
"H-NMR (DMSO-ds): 9.34 (brs, ~1H), 7.85 (brd, 2H), 7.6 (d t, 1H), 7.33 (dd, 1H), 7.06 (m, 1H), 6.77 (dt, 1H), 4.29 (m, 2H), 3.48 (m, 1H), 2.48 (m, 1 H/overlapped with DMSQ..signal), 2.00,(m, 1H). LC-MS: M+ 336
Example 51
/V-[(1g,1aff,7bf?)-4,7-d!fluoro-1,1a,2Jb-tetrahvdrQc.vclopropafc1chrornen-1-vn-
/V-(5-iodo-2-pvridinyl)urea

(Formula Remove)
o F

(1St1afl',7bS)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmol, ee -90%) was mixed with toluene (1ml), triethylamine (0.034 ml, 1.1eq), 2-amino-5-iodopyridine (54 mg, 1.1eq), DPPA (0.054 ml, 1.1 eq). The reaction mixture was then heated at stirring at 110°C for 3 h. The reaction mixture was concentrated by rotary evaporation and purified by coiumn chromatography on silica (50g, ethyiacetate/hexane 1:1) to give 35 mg..of the product as white solid, .

1H-NMR (DMSO-de): 9.4 (brs, ~1H), 8.07 (d, 1H), 8.02 (brs, ~1H), 7.91 (dd, 1 H), 7.11 (d, 1 H), 7.06 (m, 1H), 6.77 (dt, 1H), 4.29 (br d, 2H), 3.5 (m, 1H), 2.46 (m, 1 H/overlapped with DMSO signal), 2.00 (m, 1H). LC-MS: M+444.
Example 52
.A/-r(1S,1a/?.7b/Tf)-4.7-difluoro-1,1a.2.7b-tetrahvdrocycloproparc1chromen-1-vlT-
/V-(3-isoxazolvl)urea
(Formula Remove)




(1 S,1 af?,7bS)-4,7-difluoro-1,1a,2,7b-tetrahydrocyciopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmol, ee -90%) was mixed with toluene (1ml), triethylamine (0.034 ml, 1.1 eq), 3-aminoisoxazole (0.018 ml, 1.1 eq), DPPA (0.054 ml, 1.1 eq). The reaction mixture was then heated at stirring at 110°C for 3 h. The reaction mixture was concentrated by rotary evaporation and purified by column chromatography on silica (SOg, ethylacetate/hexane 1:1) to give 10 mg of the product as white solid.
1H-NMR (DMSO-d6): 9.45 (br s, ~1 H), 8.6 (d, 1 H), 7.06 (m, 1 H), 6.75 (dt, 1 H), 6.63 (d, 1 H), 6.33 (br s, ~1 H), 4.29 (m, 2H), 3.37 (overlapped with water signal), 2.43 (m, 1H), 1.98 (m, 1H). LC-MS: M+308.
Example 53
A/-f(1S.1a/:?.7bff)-4,7-difluoro-1,1a,2.7b-tetrahvdrocvGloproparc1chromen-1-vn-
A/-[4-(4-chlo.rophenvl)-1.3-thiazol-2-vllurea


(Formula Remove)
(l5,1afl,7bS)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmol, ee -90%) was mixed with toluene (1ml), triethylamine (0.034 ml, 1.1 eq), 2-amino-4-(4-chlorophenyI)-1,3-thiazole (52 mg, 1.1eq), DPPA (0.054 ml, 1.1 eq). The reaction mixture was then heated at stirring at 110°C for 3 h. The reaction mixture was concentrated by rotary
evaporation and the product was crystallized from ethanof and collected by filtration to give 50 mg of the product as white solid.
1H-NMR (CDCI3): 10,32 (brs, ~1H), 7.63 (d, 2H), 7.37 (s, 1H), 7.32 (d, 2H), 6.96 (s, 1H), 6.87 (m, 1H), 6.62 (dt, 1H), 4.44 (dd, 1H), 4.33 (dd, 1H), 3.53 (m, 1H), 2.56 (m, ~1H), 1.96 (m, 1H). LC-MS: M+434.
Example 54 . . . . • . .
A/-F(15,1aR7bfl)-4,7-difluoro-1.la,2,7b-tetrahydrocvciopropa[c]chromen-1-vn-
/V-(6-fluoro-1,3-benzothiazoi-2-vl)urea
(Formula Remove)

(lS,1a/c?,7bS)-4,7-difluora-1,1al2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmoJ, ee -90%) was mixed with toluene (1ml), triethylamine (0.034 ml, 1.1 eq), 2-amino-6-fIuoro-1,3-benzothiazole (41 mg, 1.1eq), DPPA (0.054 ml, 1.1eq). The reaction mixture was then heated at stirring-at 110°C for 3 h. The reaction mixture was concentrated by rotary evaporation and the product was crystallized from ethanol and collected by filtration to give 20 mg of the product as white solid.
1H-NMR (CDC!3):: 10.58 (br s, -1 H),.7.78 (br d, 1 HJ..7.52 (. dd, 1 H), 7.45 (dd, 1H), 7.05 (dt, 1H), 6.94 (m, 1H), 6.65 (dt, 1H),4.44 (dd, 1H), 4.33 (dd, 1H), 3.53 (m, 1H), 2.58 (m, -1H), 2.03 (m, 1H). LC-MS: M+434.

Example 55
A/-f(1 S. 1 aRJbflH.y-difluoro-l .1 a.2.7b-tetrahvdrocvclopropafc]chromen-1 -yll-
/y-(4-pyrimidinvnurea
(Formula Remove)

F O
(lS,1aff,7bS)-4,7-difluoro-1,1a,2,7b-tetrahydrocyc!opropa[c]chromene-1-carboxylic acid (50mg, 0.22mmol, ee -90%) was mixed with toluene (1ml), triethyiamine (0.034 mi, 1.1 eq), 4-aminopyrimidine (25 mg, 1.1eq), DPPA (0.054 ml, 1,1 eq). The reaction mixture was then heated with stirring at 110°C for 3 h. The reaction mixture was concentrated by rotary evaporation and the product was crystallized from ethanol and collected by filtration to give 20 mg of the product as white solid.
1H-NMR (DMSO-de): 9.71 (brs, 1H), 8.4 (brs, 1H), 8.39 (d, 1H), 7.86 (brsr 1 H), 7.31 (d, 1 H), 7.08 (m, 1 H), 6.77 (dt, 1 H), 4.31 (m, 2H), 3.48 (m, 1 H), 2.48 (m, ~1H, overlapped with DMSO signal), 2.02 (m, 1H).
Example 56
A/-f(1S,1a/:?,7bfl)-4,7-difluoro-1,1a,2.7b-tetrahvdrocvcloproparc1chromen-1-vn-/V-(2-pvrazinvl) urea
F O—-
(1S,1afl,7bS)-4,7-difluoro-1,1a,2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmoi, ee -90%) was mixed with toluene (1ml), triethyiamine (0.034 ml, 1.1 eq), 4-aminopyrazine (25 mg, 1.1eq), DPPA (0.054 ml, 1.1eq).The reaction mixture was then heated with stirring at 110°Cfor3h. The reaction mixture was concentrated by rotary evaporation and the product

was crystallized from ethanol and collected by filtration to give 5 mg of the product as white solid.
1H-NMR (DMSO-de): 9.57 (br s, 1H), 8.67 (br s, 1H), 8.10 (d, 1H), 7.95 (br s, 1 H), 7.64 (br s, 1 H), 7.05 (m, 1 H), 6.77 (dt, 1 H), 4.31 (m, 2H), 3.49 (m, 1 H), 2.48 (m, ~1H, overlapped with DMSO signal), 2.02 (m, 1H).
Example 57
A/-IY1 'S, 1 a/TfJbflM.Z-difluoro-l, 1 a,2.7b-tetrahvdrocvclopropa|'c]chrorrien-1 -yll-
/V-(5-cvclopropyl-1/-y-pvrazol-3-vl)urea
(Formula Remove)

F
(1S,1a/:?,7bS)-4,7-difluoro-1,1a>2,7b-tetrahydrocyclopropa[c]chromene-1-carboxylic acid (50mg, 0.22mmol, ee -90%) was mixed with toluene (1ml), triethylamine (0.034 ml, 1.1 eq), 3-amino-5-cyclopropyl-1/-/-pyrazole (30 mg, 1.1 eq), DPPA (0.054 ml, 1.1eq). The reaction mixture was then heated at stirring at 110°C for 3 h. The reaction mixture was concentrated by rotary svaporation and two compounds were separated by column chromatography Dn silica (50g, ethylacetate/hexane 1:3) to give 3 mg of the title product. The structure assignment was proved by 13C, gHMBC, gHMQC and NOESY NMR experiments.
1H-NMR (CDCI3): 7.05 (brd, ~1H), 6.88 (m, 1H), 6.64 (dt, 1H), 5.24 (d, 1H), 4.49 (dd, 1H), 4.33 (dd, 1H), 3.63 (m, 1H), 2.61 (m, ~2H), 1.99 (m, 1H), 0.99 (m, 2H), 0.58 (m, 2H).
Biological results
Extensive guidance on the assay of test compounds at the enzyme level and in cell culture, including the isolation and/or selection of mutant HIV strains and mutant RT are found in DAIDS Virology Manual for HIV Laboratories

complied by Division of AIDS, NIAID USA 1997. Resistance studies, including rational for various drug escape mutants is described in the HIV Resistance Collaborative Group Data Analysis Plan for Resistance Studies, revised 31 August 1999.
Compounds of the invention are assayed for HIV activity, for example using multiple determinations with XTT in MT-4 cells (Weislow et al, J Nat Cancer Inst 1989, vol 81 no 8, 577 et seq), preferably including determinations in the presence of 40-50% human serum to indicate the contribution of protein binding. In short the XTT assay uses human T cell line MT4 cells grown in RPMI 1640 medium supplemented with 10% fetal calf serum (or 40-50% human serum as appropriate), penicillin and streptomycin seeded into 96 well microplates (2»104 cells/well) infected with 10-20 TCID50 per well of HlV-1mB (wild type) or mutant virus, such as those bearing RT lie 100, Cys 181 or Asn 103 mutations. Serially diluted test compounds are added to respective wells and the culture incubated at 37°C in a COa enriched atmosphere and the viability of cells is determined at day five or six with XTT vital dye. Results are typically presented as ED5o u.M.
Compounds of the invention were assayed in the above XTT assay using wild type HIV-1 ma as shown in Table I:

Example

EDso (nM)



Example 7

7

Example 16
Example 18

Example 19

10



Example 20

7



Example 23

7



Example 24

20

Example 30

Example 31

2.5

Example 33
Example 43
Compounds are preferably potent against wild type virus and mutant HIV virus, especially virus comprising drug escape mutations. Drug escape mutations are those which arise in patients due to the selective pressure of a prior art antiviral and which confer enhanced resistance to that antiviral. The above cited Data Analysis Plan outlines relevant drug escape mutants for each of the antiviral classes currently on the market. Drug escape clones are readily isolated from HIV patients who are failing on a particular antiviral therapy. Alternatively the preparation of RT mutations on a known genetic background is shown in WO97/27319, WO99/61658 and WOOO/73511 which also show the use of such mutants in sensitivity profiling.
K103 N is a particularly relevant drug escape mutant in the context of NNRTl therapy and compounds of the invention preferably have a low ED5Q against this mutant, especially in assays mimicking the presence of human serum. Compounds of the invention, such as those exemplified above show sub micrornolar activities in such assays.




We Claim:
1. Cis-l-(5-Cyano-pyridin-2 - yl) - 3 - (4, 7-difluoro-1, 1a, 2, 7b-tetrahydro cyclopropa
[c]chromen-l-yl)-urea of formula I,
(Formula Removed)
where;
R1 is 0;
R2 is 5-cyano-pyrid-2-yl;
R3 is H;
R4 and R7 are F;
R5 and R6 are H;
X is — O-CH2—;
or pharmaceutically acceptable salts thereof.
2. A compound as claimed in claim 1, wherein the cyclopropyl moiety has an enantiomeric
excess of the conformation depicted in the partial formulae:
(Formula Removed)
where X is as defined, Y is the bridge to the (substituted) phenyl ring depicted in formula I and Z is bond to the (urea)-R2 depicted in formula I.
3. A compound as claimed in claim 1 wherein the compound of formula I comprises an enantiomeric excess of the isomer showing negative optical activity.

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Patent Number 246948
Indian Patent Application Number 01281/DELNP/2003
PG Journal Number 12/2011
Publication Date 25-Mar-2011
Grant Date 22-Mar-2011
Date of Filing 13-Aug-2003
Name of Patentee MEDIVIR AB
Applicant Address LUNASTIGEN 7, S-14144 HUDDINGE. SWEDEN
Inventors:
# Inventor's Name Inventor's Address
1 STEFAN LINDSTROM GUSTAV KJELLBERGSVAG 2, S-75643 UPPSALA, SWEDEN
2 CHRISTER SAHLBERG MALARHOJDSVAGEN 52, S-14940 HAGERSTEN, SWEDEN
3 HANS WALLBERG MANGARDSVAGEN 10, S-14151 HUDDINGE, SWEDEN
4 GENAIDY KALYANOV BJURSATRAGATAN 57, 12464 BANDHAGEN, SWEDEN
5 LOURDES ODEN SALTANGSGATAN 1B, NORRKOPING, SWEDEN 60222, SWEDEN
6 LOTTA NAESLUND VADERKVARNSGATAN 42C, S-75326 UPPSALA, SWEDEN
PCT International Classification Number C07D 407/12
PCT International Application Number PCT/EP02/02328
PCT International Filing date 2002-03-04
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 0100733-5 2001-03-05 Sweden