Title of Invention

NOVEL AMINE SALTS OF CEPHALOSPORIN AND ITS USE IN THE PREPARATION OF CEPHALOSPORIN ANTIBIOTICS

Abstract The present invention relates to an improved process for the preparation of cephalosporin antibiotics of formula (I). The present invention also provides new salts of compound of formula (II) and a process for the preparation of these new salts. where n = 0.5 to 2 The present invention also provides a process for the preparation of compound of formula (I) using the new salts of formula (II).
Full Text

Field of the Invention
The present invention relates to an improved process for the preparation of cephalosporin antibiotics of formula (I)

wherein R represents hydrogen pharmaceutically acceptable alkali metal salts, or pharmaceutically acceptable esters, R1 represents

R4 represents hydrogen or -CHO group; R5 represents hydrogen, trityl, CH3, CRaRbCOORc where Ra and Rb independently represent hydrogen or methyl and Re represents hydrogen or (C1-C6)alkyl; Z represents hydrogen, C 1.6 alkyl, and substituted or unsubstituted aryl; R2 represents hydrogen or methoxy group; R3 represents hydrogen, -CH3, -CH2OCOCH3, -CH=CH2, -CH2-0-CO-NH2 , -CH=CH-CH3 or a group represented by


The present invention also provides new salts of compound of formula (II)
where n = 0.5 to 2 wherin R1, R2 and R3 are defined as above and M represents the compound of formula (III)
wherein R6, R7 may be identical or different and are each, independently of one another, represent a linear or branched alkyl having from 1 to 8 carbon atoms or together with the C atom to which they are bound form a 3- to 8- membered saturated ring which may be further substituted or substituted aryl ring;
The present invention also provides a process for the preparation of compound of formula (I) using the new salts of formula (II).

Background of the Invention
US patent No. 6,350,869 discloses the purification of impure cefdinir through the preparation of N,N-dicyclohexylamine salt of 7-[2-aminothiazol-4-yl-2-(z)-hydroxyimino acetamido]-3-vinyl-3-cephem-4-carboxylic acid and subsequent hydrolysis to get pure cefdinir. This process requires the preparation of crude cefdinir, conversion to N,N-dicyclohexylamine salt and then hydrolysis of the salt to get pure cefdinir, and therefore the overall yield is not attractive.
US patent 5,705,496 discloses a process for preparing the benzathine salt of Cefonicid which comprises treating an aqueous solution or suspension of Cefonicid with an organic solvent and N,N'-dibenzylethylenediamine.
US patent 5,026,843 discloses the process for the preparation of Ceftriaxone disodium hemiheptahydrate involving condensing 7-ACT with MAEM. The product formed is precipitated by using dibenzylethylenediamine and subsequently is treated with sodium ethylhexanoate in acetone.
In order to get rid of impurities and isolating the product from aqueous medium, all these prior art references used different kind of amine salt with cephem-4-carboxylic acid derivatives. But none of the processes gave desired yield and quantity.
In continuation of our research, for developing new and efficient processes for making cephlosporins, we have found new salts of formula (II) and its use in the preparation of cephalosporin antibiotics of the formula (I) with minimum impurities in high yield.

Objectives of the Invention
The main objective of the present invention is to provide new salts of compound of formula (II), which are stable throughout the process for the preparation of cephalosporin antibiotics of the formula (I).
Another objective of the present invention is to provide a process for the preparation of cephalosporin antibiotics of formula (I) using these new salts.
Another objective of the present invention is to prepare cephalosporin antibiotics of formula (I) that are highly pure, high yielding and cost effective, which would be easy to implement on manufacturing scale.
Still another objective of the present invention is to provide a process for the preparation of new salts of formula (II).
Summary of the Invention
Accordingly, the present invention provides new salts of the compound of formula (II),
where n= 0.5 to 2 wherein R1, R2, R3 are as defined above; and M represents the compound of formula (HI),

wherein R6, R7 may be identical or different and are each, independently of one another, represent a linear or branched alkyl having from 1 to 8 carbon atoms or together with the C atom to which they are bound form a 3- to 8- membered saturated ring which may be further substituted or substituted aryl ring;
Another embodiment of the present invention is to provide a process for the preparation of compound of the formula (I), which comprises i) condensing the compound of the formula (IV) or its salts or its trimethylsilyl esters with compound of formula (V) wherein Y is a group which forms a basis that a compound of formula (V) is in a reactive form ; including halogen , a group which forms together with the -C=0 group to which Y is attached an active ester, thioester, and a group which forms together with the -C=0 group to which Y is attached a mixed anhydride in the presence or absence of a base and solvent to produce compound of formula (VI),
ii) treating the compound of formula (VI) with compound of formula (III) to produce the compound of formula (II), and
iii) treating the compound of formula (II) either with an acid or with sodium exchanging reagent to produce the compound of formula (I). The process is shown in the Scheme-I


Detailed description of the invention
In an embodiment of the present invention, the base used in step (i) is selected from sodium hydroxide, sodium acetate, potassium hydroxide, ammonium hydroxide, sodium carbonate, potassium carbonate, sodium bicarbonate triethylamine, trimethylamine, N-methylpiperidine, diethylamine, tributylamine, pyridine, N-alkylanilines, N,N-diisopropylethylamine N-methylmorpholine, and the like or mixtures thereof
In another embodiment of the present invention the reaction in step (i) is carried out at a temperature ranging from -50°C to + 80°C.
In an another embodiment of the present invention, the solvent used in step (i) is selected from ethanol, methanol, isopropanol, THF, cyclohexanol, acetonitrile, DMAc, DMF, N-methyl pyrrolidine, ethyl acetate, methylene dichloride, ethylene dichloride, acetone, toluene, tetrahydrofuran, xylene, water or mixtures thereof
In yet another embodiment of the present invention, Y is a group which forms a basis that a compound of formula (V) is in a reactive form; including chlorine, bromine, fluorine and iodine, a group which forms together with the-C=0 group to which Y is attached an active ester, thioester, and a group which forms together with the -C=0 group to which Y is attached a mixed anhydride. Illustrative example of those active esters includes benzothiazole, benzoxazole, benzotriazole or benzimidazole.
In yet another embodiment of the present invention, M is selected from N,N'-Diisobutylethylenediamine, N,N'-Dicyclohexyllethylenediamine or N,N'- Di-(p-anisyl)lethylenediamine, N,N'-Dicyclopentylethylenediamine and N,N'- (p-tolyl)lethylenediamine
In yet another embodiment of the present invention, the solvent used in step (ii) is selected from ethanol, methanol, isopropanol, acetonitrile, acetone, ethylacetate, THF, DMAc, DMF, water or mixtures thereof.

In still another embodiment of the present invention the acid employed in step (iii) is selected from HCl, sulfuric acid, formic acid, acetic acid, perchloric acid, benzenesulfonic acid, p-toluenesulfonic acid, naphthalenesulfonic acid, methanesulfonic acid, trifluoro acetic acid, UBK530 resin, cationic exchange resin or mixtures thereof.
In still another embodiment of the present invention, wherein the sodium exchanging reagent is selected from sodium acetate, sodium citrate, sodium lactate, sodium ethylacetoacetate and sodium 2-ethylhexanoate and the solvent used for dissolving the reagent is selected from ethanol, methanol, isopropanol, acetonitrile, ethyl acetate, acetone, water or mixtures thereof.
In another embodiment of the present invention the novel salts prepared in accordance with this invention is useful in the isolation of cephalosporin antibiotics such as Cefonicid ,Cefdinir ,Cefoxitin,Cefditoren,Cefixime ,Cefepime in pure and good yield.
In one more embodiment of the present invention the starting material of formula (IV) is prepared by utilizing the scheme available in the prior art.
The present invention is illustrated with the following examples, which
should not be construed as limiting to the scope of the invention.
Example 1
Step-(i)
N,N'-Diisobutylethylenediamine salt of 7-D-mandelamido-3-(((l-
sulfomethyl-iH-tetrazol-5-yI)thio)metliyl)-3-cephem-4-carboxylic
acid
7-amino-3-( 1 -sulfomethvl-lH-tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid (5 g) was dissolved in water (19 ml) using 30% w/w sodium hydroxide (1.5 g in 8 ml water) and sodium bicarbonate (1 g), O-formylmandeloyl chloride (2.45 g) was added at O-5 C and maintained at 5-10"C over a period of 1-2 hours. After the

completion of reaction, the reaction mixture was acidified with conc. HCl (2.6 g) and maintained the reaction at 28-30'C for about 2-3 hrs, sodium bicarbonate (~ 3.5 g) was added to set the pH 5+1 and the reaction mass solution was added to a solution of N,N'-diisobutylethylenediamine diacetate (8.2 g) in water (40 mL) and isopropyl alcohol (40 mL) and stirred well at 25-30"C for about 2-3 hours. The reaction mixture was cooled to 5-10'C5 filtered, washed with chilled water (0-5"C) followed by isopropyl alcohol (0-5"C) and dried the material at - 40°C to get N,N'-diisobutylethylenediamine salt of 7-D-mandelamido-3-(((l-sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid (5.5 g).
Stepdi)
7-D-Mandelamido-3-(((l-sulfomethvl-lH-tetrazol-5-yl)thio)methvn-3-cephem-4-carboxvlic acid, sodium salt
N,N'-diisobutylethylenediamine salt of 7-D-mandelamido-3-(((l-sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid (2.0 g) was charged into a mixture of isopropyl alcohol (20 mL) and acetone (4 mL) followed by UBK-530 resin (5 g) and stirred the reaction mixture for 2-4 hours at 25-30'C. The reaction mixture was filtered and washed the resin using acetone(10 mL). To the clear filtrate, a solution of sodium 2-ethylhexanoate (LO g in 5 ml acetone) was added at 25-30 C, stirred for 1 hour, filtered, washed with acetone, and dried the material under vacuum to yield 7-D-mandelamido-3-((( 1 -sulfomethvl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid, sodium salt (L4 g).
Example 2;
Step-m
N,N'-Dicvclohexvlethvlenediamine salt of 7-D-mandelamido-3-(((1-
sulfomethvl-J.H-tetrazol-5-vl)thio)methyl-3-cephem-4-carboxvlic acid
7-amino-3-(sulfomethyl-lH-tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid

(5 g), was dissolved in water (19 ml) using 30% w/w sodium hydroxide (1.5 g in 8 ml water) and sodium bicarbonate (1 g), 0-formylmandeloyl chloride (2.45 g) was added at 0-5 C and maintained at 5-10 C over a period of 1-2 hours. After the completion of reaction, the reaction mixture was acidified with cone. HCl (2.6 g) and maintained the reaction at 28-30 C for about 2-3 hours. After the completion of reaction, sodium bicarbonate (-- 3.5 g) was added to set the pH 5+1 and the solution was added to a solution of N,N'-dicyclohexylethylenediamine diacetate (9.26 g) in water (40 mL) and isopropyl alcohol (40 mL) and stirred well at 25-30°C for about 2-3 hours. The reaction mixture was cooled to 5-10 X, filtered, washed with chilled water (0-5'C) and isopropyl alcohol (0-5'C) and dried the material under vacuum at ~40'*C to get N,N'-dicyclohexylethylenediamine salt of 7-D-mandelamido-3-( 1 -sulfomethyl-1 H-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid (6.5 g). Step-(ii)
7-D-MandeIamido-3-(((l-sulfomethvl-lH-tetrazol-5-ynthio)methvl)-3-cephem-4-carboxvlic acid, sodium salt
N,N'-dicyclohexylethylenediamine salt of 7-D-mandelamido-3-(((l-sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid (2.0 g) was charged into a mixture of isopropyl alcohol (20 mL) and acetone (4 mL) followed by UBK-530 resin (5 g) at 25-30"C and stirred for 2-4 hours , filtered the reaction mass and washed the resin with acetone (10 mL). To the filtrate, a solution of sodium 2-ethylhexanoate (0.97 g in 5 ml acetone) was added at 25-30'C and stirred for about 1 hour, filtered, washed with acetone, and dried the material under vacuum to yield 7-D-mandelamido-3-(((l-sulfomethyl-iH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid, sodium salt (1.4 g). Example 3: Stepd)

N.N'-Di-(p-anisvDethvlenediamine salt of 7-D-mandelamido-3-(((l-sulfomethvl-iH-tetrazol-5-vl)thio)inethvn-3-cephem-4-carboxvlic acid
7-amino-3-( 1 -sulfomethyl-1 H-tetrazol-5-yl)thiomethvl-3-cephem-4-carboxvlic acid (5 g) was dissolved in water (19 ml) using 30% w/w sodium hydroxide (1.5 g in 8 ml water) and sodium bicarbonate (1 g), o-formylmandeloyl chloride (2.45 g) was added at O-5°C and maintained at 5-10°C over a period of 1-2 hours. After the completion of reaction , the reaction mixture was acidified with cone. HCl (2.6 g) and maintained under stirring at 28-30°C over 2-3 hours. After the completion of reaction, sodium bicarbonate (-- 3.5 g) was added to set the pH 5+1. The solution was added to a solution of N,N'-di-(p-anisyl)ethylenediamine diacetate (11.7 g) in water (40 mL) and isopropyl alcohol (40 mL) and stirred well at 25-30 "C. After stirring for 2-3 hours, the reaction mixture was cooled to 5-10°'C, filtered, washed with chilled water (0-5 "C) and isopropyl alcohol (0-5 "C) and dried the material under vacuum at ~ 40°C to get N,N'-di-(p-anisyl)ethylenediamine salt of 7-D-mandelamido-3-(((l-sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid (6.4 g).
step-(ii)
7-D-Mandelamido-3-(((l-Sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxvlic acid, sodium salt
N,N'-di-(p-anisyl)ethylenediamine salt of 7-D-mandelamido-3-((( 1 -sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxyUc acid (2.0 g) was charged into a mixture of isopropyl alcohol (20 mL) and acetone (4 mL) followed by UBK-530 resin (5 g) at 25-30°C and stirred for 2-4 hours. The reaction mixture was filtered, washed with acetone (10 mL). To the filtrate, a solution of sodium 2-ethylhexanoate (0.86 g in 5 ml acetone) was added at 25-30°C, stirred for 1 hour, filtered, washed with acetone, and dried the material under vacuum to yield 7-D-

mandelamido-3-(((l-sulfomethyl-lH4etrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid, sodium salt(1.5g). Example;4 Step-i
Preparation of N,N'-dicvclohexvlethane-1.2-diammonium 7B-f2-(2-amino-4-thiazolyn-2-(Z-tritvIoxyimino)acetamido1-3-vinvl>3-cephem-4-carboxvlate 7-amino-3-vinyl-3-cephem-4-carboxylic acid (10 gm) was taken in a mixture of tetrahydrofuran (60 ml) and water (5 ml), triethylamine (9.0 gm) was added drop-wise at 20±2 °C over 30-45 minutes. 2-mercaptobenzothiazolyl (Z)-(2-aminothiazol-4-yl)-2-(trityloxyimino)acetate (26 gm) was added to the clear solution and the resulting mixture was stirred at 32+2 °C for 4-6 hours. After completion of the reaction, the reaction mixture was cooled to 20±2 °C, ethyl acetate (200 ml) and water (250 ml) were added and stirred for 10 min. The pH of the reaction mixture was adjusted to 3.50-4.00 by 1:1 HCl (20.0 ml) in 30 min. The layers were separated; ethyl acetate layer was washed with 20% sodium chloride solution (20 g of NaCl in 100 ml of water) and separated. The ethyl acetate layer was added dropwise in 20 min at 32±2 °C to a solution of N,N'-dicyclohexylethane-1,2-diamine in a mixture of acetonitrile (300 ml) and methanol (50 ml) and stirred for 25 min. The resulting slurry was filtered, washed with acetonitrile (100 ml) and dried at 30-35 *^C under vacuum to obtain N,N'-dicyclohexylethane-l,2-
diammonium 7P-[2-(2-amino-4-thiazolyl)-2-(Z-trityloxyimino)acetamido]-3-vinyl-
3-cephem-4-carboxylate (30.1 gm, HPLC Purity 93.74 %)
Step-ii
Preparation of N,N'-dicyclohexylethane"l,2-diammonium 7P-[2-(2-ainino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vmyl-3-cephem-4-carboxylate from N,N'-dicyclohexylethane-l,2-diammonium 7p-[2-(2-amino-4-thiazoIyl)-2-(Z-trityloxyimino) acetamido]-3-vinyl-3-cephem-4-carboxylate

A mixture of N,N'-dicyclohexylethane-l,2-diammonium 7p-[2-(2-amino-4-thiazolyl)-2-(Z-trityloxyimino) acetamido]-3-vinyl-3-cephem-4-carboxylate (50 gm) and 2.5 gm of activated carbon was added to a mixture of acetonitrile (150 ml) and water (21 ml) and heated to 50 °C. The reaction mixture was heated to 60-62 °C in 20 minutes, hydrochloric acid (34 ml, 1:1 v/v) and maintained at this temperature for 35 minutes. At 36th minute 300 ml of chilled 1;1 mixture of acetone and isopropanol having temperature -15 °C was added to the reaction mixture to reduce the temperature to 30-35 °C. The carbon was filtered and the carbon bed was washed with acetone (100 ml). A methanolic solution of N,N'-dicyclohexylethane-l,2-diamine (10 gm in 25 ml of methanol) was added to the filtrate dropwise over 30 minutes to adjust the pH of the solution to 6.5 -6.75 at 35 °C and stirred for 30 minutes. The resulting slurry was stirred for 15 - 20 minutes at 33-35 °C, cooled to 25 °C, and stirred for 30 minutes. The product thus obtained was filtered, washed with 100 ml of acetone and dried at 35°C under vacuum for 3-4 hours to get the 40.0 gm of N,N'-dicyclohexylethane-l,2-diammonium 7P-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate (HPLC purity Step-iii
Preparation of 7-[[(2-aminothiazol-4-yl)-(z)(hydroxyimino)] acetyl]amino-3-vinyl-3-cephem-4-carboxylic acid from N,N'-dicyclohexylethane-l,2-diammonium 7p-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vinyl-3-cepliem-4-carboxylate
N,N'-dicyclohexylethane-l,2-diammoiiiuin 7-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vinyl-3-cephein-4-carboxylate (20 gm) was

added to a solution of acetone 80 ml in water 600 ml at 32-36 °C, dissolved by hydrochloric acid (25 ml) at pH 1.25 to 1.75 in 5-10 minutes, and adjust the pH to 5.4-5.6 by ammonia (15 ml 15%). The aqueous solution was treated with carbon and EDTA at 30-34 °C for 30 min. Carbon was filtered, washed with water (80 ml) and the pH was adjusted to 2.5-2.6 by 20% sulphuric acid cooled to 25-27 °C. After 30 minutes being stirred, the product was filtered, washed with 50 ml of cold-water, followed by acetone (25 ml) and dried in air at 28-32 °C for 3-5 hrs to get 7.95 gm of 7-[[(2-aminothiazol-4-yl)-(z)(hydroxyimino)] acetyl]amino-3-vinyl-3-cephem-4-carboxylic acid [HPLC quality >99.0 %]. Example;5
Preparation of N,N'-dicycIohexylethane-l,2-diammonium 7P-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate from potassium 7P-[2-(2-amino-4-thiazolyl)-2-(Z-trityloxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate
A mixture of 50 gm of potassium 7-[[2-(2-aminothiazol-4-yl)"(z)-2-(trityloxyimino)]acetyl]amino-3-vinyl-3-cephem"4-carboxylate and carbon (2.5 gm) was added to a mixture of acetone 55 ml and water 21 ml and heated to 50 °C. The reaction mixture was heated to 60-62 °C in 20 minutes, hydrochloric acid (34 ml, l:lv/v) and maintained at 65-67 °C for 35 minutes. At 36th minute, a 1:1 mixture of 400 ml of acetone and isopropanol having temperature -15 °C was added to the reaction mixture to reduce the temperature to 30-35 °C. The carbon was filtered and the carbon bed was washed with acetone (100 ml). A methanolic solution of N, N'-dicyclohexylethane-1, 2-diamine (10 gm in 25 ml of methanol) was added to the filtrate dropwise over 30 minutes to adjust the pH of the solution to 6.5 -7.0 at 35 °C and stirred for 30 minutes. The resulting slurry was stirred for 15 - 20 minutes at 33-35 °C, cooled to 25 °C, and stirred for 30 minutes. The product thus obtained was filtered, washed with 100 ml of acetone and dried at 35°C under vacuum for 3-

4 hours to get the 36.0 gm of N, N'-dicyclohexylethane-l, 2-diammonium 7p-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate
(HPLC purity Example: 6
Preparation of N,N'-dicyclohexylethane-l,2-diammonium 7p-[2-(2-amino-4-
thiazolyl)-2-(Z-hydroxyimino)acetamidol-3-vinyl-3-cephem-4-carboxylate from
dicyclohexylammonium 7p-[2-(2-amiiio-4-thiazolyl)-2-(Z-trityloxyimino) acetamido]-
3-vinyl-3-cephem-4-carboxylate
A mixture of dicyclohexylammonium 7p-[2-(2-amino-4-thiazolyl)-2-(Z-trityloxyimino) acetamido]-3-vinyl-3-cephem-4"Carboxylate (50 gm) and 2.5 gm of activated carbon was added to a mixture of acetonitrile (150 ml) and water (21 ml) and heated to 50 °C. The reaction mixture was heated to 60-62 °C in 20 minutes, hydrochloric acid (34 ml, l:lv/v) and maintained at 65-67 °C for 35 minutes. At 36 minute, a chilled mixture of 300 ml of acetone and isopropanol having temperature -15 °C was added to the reaction mixture to reduce the temperature to 30-35 °C. The carbon was filtered and the carbon bed was washed with acetone (100 ml). A methanolic solution of N,N'-dicyclohexylethane-l,2-diamine (10 gm in 25 ml of methanol) was added to the filtrate dropwise over 30 minutes to adjust the
pH of the solution to 6.5 -7,0 at 35 °C and stirred for 30 minutes. The resulting slurry was stirred for 15 - 20 minutes at 33-35 °C, cooled to 25 °C, and stirred for 30 minutes. The product thus obtained was filtered, washed with 100 ml of acetone and dried at 35°C under vacuum for 3-4 hours to get the 42.0 gm of N,N'-
dicyclohexylethane-l,2-diammonium7p-[2-(2-amino-4-thiazolyl)-2-(Z-
hydroxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate.
Example 7
N,N'-Diisopentylethylenediamine salt of 7-D-mandelamido-3-(((l-
sulfomethyl-7H-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic
acid

7-amino-3-(l-sulfomethyl-iH-tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid (25 g) was dissolved in water (110 ml) using sodium bicarbonate (4 g) at 0-5°C in 2.0 hrs , 0-formylmandeloyl chloride (12.5 g) was added at 0-5°C and maintained at 5-10'C over a period of 0.5-1.0 hours. After the completion of reaction, the reaction mixture was acidified with cone. HCl (15-16mL) and maintained the reaction at 28-30°C for about 2-3 hrs. To the reaction mass , mixture of Methylene dichloride and Tetrahydrofuran are charged and separated the aqueous layer. Aqueous layer pH was adjusted to 4.0 using 15 % ammonia solution at 28-30 "C for a period of 0.5-1.0 hour .The reaction mass solution was added to a solution of N,N'-diisopentylethylenediamine diacetate (20.2 g) in water (88 mL) in presence of Tetrahydrofuran and stirred well at 25-30"C for about 2-3 hours. The reaction mixture was cooled to 5-10°C, filtered, washed with chilled water (0-5°C) and dried the material at ~ 40*^0 to get N,N'-diisopentylethylenediamine salt of 7-D-mandelamido-3-(((l-sulfomethyl-lH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid (38 g). Example 8
N5N'-(p-tolyl)ethylenediamine salt of 7-D-mandelamido-3-(((l-
sulfomethyl-iH-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic
acid
7-amino-3-(l-sulfomethyl-lH-tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid
(25 g) was dissolved in water (110 ml) using sodium bicarbonate (4 g) at 0-5°C in 2.0 hrs , 0-formylmandeloyl chloride (12.5 g) was added at 0-5°C and maintained at 5-10°C over a period of 0.5-1.0 hours. After the completion of reaction, the reaction mixture was acidified with cone. HCl (15-16mL) and maintained the reaction at 28-30"C for about 2-3 hrs. To the reaction mass , mixture of Methylene dichloride and Tetrahydrofuran are charged and separated the aqueous layer. Aqueous layer pH was adjusted to 4.0 using 15 % ammonia solution at 28-30 °C for a period of 0.5-1.0 hour .The reaction mass solution was added to a solution of

N,N'(p-tolyl)ethylenediamine diacetate (23 g) in water (92 mL) in presence of
Tetrahydrofuran and stirred well at 25-30°C for about 2-3 hours. The reaction
mixture was cooled to 5-10°C, filtered, washed with chilled water (0-5°C) and dried
the material at -- 40°C to get N,N'-(p-tolyl)ethylenediamine salt of 7-D-
mandelamido-3-((( 1 -sulfomethyl-1 H-tetrazol-5-yl)thio)methyl)-3 -cephem-4-
carboxylic acid (38 g).
Example 9
Preparation of 7P-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-
vinyl-3-cephem-4-carboxylic acid (cefdinir)
Step-i Preparation of N,N'-Dicvclohexvlethvlenediamine salt of 7B-[2-(2-amino-4-
thiazolyl)-2-(Z*hydroxyimino)acetamidol-3-vinyl-3-cephem-4-carboxvlic acid
(Cefdinir -PDA)
To a chilled suspension of 7-amino-3-vinyl-3-cephem-4-carboxylic acid (50 gm) in a mixture of tetrahydrofiiran (250 ml) and water (31.5 ml), triethylamine (45 gm) was added drop-wise at 20±2 °C over 30-45 minutes. 2-mercaptobenzothiazolyl (Z)-(2-aminothiazol-4-yl)-2-(trityloxyimino)acetate (130 gm) was added to the clear solution and the resulting mixture was stirred at 32 ±2 °C for 4-6 hours. The reaction was monitored by HPLC.After the completion of reaction, THF was completely removed under vacuum at 28-30°C. To the concentrated mass, acetone (300mL) was added and stirred at 30 °C, 1:1 Hydrochloric acid (200mL) was added and the temperature was raised to 63-64 °C. At this temperature, it was refluxed for about 35 min and then cold acetone of 1.8 Litre dumped to the reaction mass to bring down the temperature to 30 °C, EDTA (0.5g ) was added and stirred for 10 min at 30-35°C, pH of the solution was adjusted to 2.5 using Triethyl amine (lOOmL) at 30-35 °C .To the reaction mixture hot solution of N,N'-Dicyclohexylethylenediamine ( DDA) 40.0g in Isopropyl alcohol (100mL) was added slowly to adjust the pH of the solution 5.5 and stirred for 1.0 hour at 30-35°C

. The precipitated material was filtered, washed with acetone and dried under vacuum at at ~ 40'C to get N,N'-Dicyclohexylethylenediamine salt of 7p-[2-(2-amino-4-thiazolyl)-2-(Z-hydroxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylic acid. Step-ii
Preparation of Cefdinir from Cefdinir -PDA
Cefdinir -DDA (lOOg) was stirred in a mixture of acetone (200mL) and water (3.0 Lt) at 35-38°C,pH of the solution was adjusted to 1.5 using 1: 1 HCl in 5-10 minutes then pH of the solution was readjusted to 6.0 using aqueous ammonia solution (70 mL) at 35-38 °C. To the resulting solution, carbon (10g) was added stirred for 20 -25 minutes and filtered. pH of the clear filtrate was adjusted to 2.5 with 1:1 hydrochloric acid (50mL) to precipitate Cefdinir. The precipitate was stirred for 3.0 hrs at 30-35°C, filterd and washed the wet cake with water (500mL). Wet material is dried under vacuum to get 46.0 g of Cefdinir.





We claim:
1. A compound of general fomula (II)

where n=0.5 to 2 (pl change and include suffix) wherein R1 represents

R4 represents hydrogen or -CHO group, R2 represents hydrogen or methoxy group, R3 represents hydrogen,-CH3,-CH20COCH3, -CH=CH2,-CH2-O-CO-NH2 , -CH=CH-CH3


Z represents hydrogen,C 1.5 alkyl ,substituted or unsubstituted aryl.
wherein R5 represents hydrogen, trityl, CH3, CRaRbCOORc where Ra and Rb
independently represent hydrogen or methyl and Rc represents hydrogen or (C1-
C6)alkyl.
M represents the formula
wherin R1,R2 and R3 are defined as above .
wherein R6, R7 may be identical or different and are each, independently of one another, represent a linear or branched alkyl having 1 to 8 carbon atoms or together with the C atom to which they are bound form a 5- to 8- membered saturated ring which may be further substituted or substituted aryl ring;.
2. A compound as claimed in claim 1, wherein the compound is


where n =0.5-2
wherein M represents N,N'-Diisobutylethylenediamine, N,N'-
Dicyclohexyllethylenediamine , N,N'- Di-(p-anisyl)lethylenediamine, N,N'-Dicyclopentylethylenediamine and N,N'- (p-tolyl)lethylenediamine .
3. A compound as claimed in claim 1, wherein the compound is

where n =0.5 to 2 wherein M is as defined in claim (2).
4. A compound as claimed in claim 1, wherein the compound is

wherein M is as defined in claim (2).

5. A process for the preparation of compound of formula (II), which comprising the
steps of:
i) condensing the compound of the formula (VI)
R4
COOH
(VI) (VII)
with compound of the formula (VII) wherein Y is a group which forms a basis that
a compound of formula (VII) is in a reactive form ; including halogen, or a group
which forms together with the -C=0 group to which Y is attached an active ester,
and a group which forms together with the -C=0 group to which Y is attached a
mixed anhydride in the presence of a base and solvent to produce the compound of
formula (VIII), and

HR2 R1—CO-N :

COOH

(VIII)

ii) treating the compound of formula (VIII) with compound of formula (M) to
produce the compound of formula (II).
6. The process according to claim 5, wherein M is selected from N,N'-
Diisobutylethylenediamine , N,N'- Dicyclohexylethylenediamine or N,N'- Di-
(p-anisyl)lethylenediamine.
7) The process according to claim 5, wherein the base used in step (i) is selected
from sodium hydroxide, sodium acetate, potassium hydroxide, ammonium
hydroxide,sodium carbonate, potassium carbonate, sodium bicarbonate
triethylamine, diethylamine, tributylamine, pyridine, N-methylanilines, N-
methylmorpholine and mixtures thereof.

8) The process according to claim 5, the solvent used in step (i) is selected from ethanol, methanol, isopropanol, THF, cyclohexanol, acetonitrile, DMAc, DMF, N-methyl pyrrolidine, ethyl acetate, methylene dichloride, ethylene dichloride, acetone, toluene, tetrahydrofuran, xylene, water or mixtures thereof.
9) A process according to claim 6, further comprising converting the compound of formula (II) into compound of formula (I) by dissolving the compound of formula (II) in a mixture of organic solvent followed by treating the clear solution with an acid or with sodium exchanging reagent to produce the compound of formula (I).

10) The process of claim 9, wherein the sodium exchanging reagent is selected from sodium acetate, sodium citrate, sodium lactate, sodium ethylacetoacetate and sodium 2-ethylhexanoate and the solvent used for dissolving sodium exchange reagent is selected from ethanol, methanol, isopropanol, acetonitrile, ethyl acetate, acetone, water or mixtures thereof.
11) The process of claim 9, wherein the acid is selected from HCl, sulfuric acid, formic acid, acetic acid, perchloric acid, benzenesulfonic acid, p-toluenesulfonic acid, naphthalenesulfonic acid, methanesulfonic acid, trifluoro acetic acid,cationic exchange resin selected from UBK530,UBK 535,UBK 550,UBK 555,PK 220,
PK 228,PK216 resin or mixtures thereof


Documents:

637-che-2004 amended claims 28-10-2010.pdf

637-CHE-2004 CORRESPONDENCE OTHERS 28-10-2010.pdf

637-CHE-2004 EXAMINATION REPORT REPLY RECEIVED 16-07-2010.pdf

637-CHE-2004 FORM-13 28-10-2010.pdf

637-che-2004 form-3 16-07-2010.pdf

637-CHE-2004 AMANDED CLAIMS 02-02-2010.pdf

637-CHE-2004 AMANDED PAGE OF SPECIFICATION 02-02-2010.pdf

637-CHE-2004 CORRESPONDENCE-OTHERS 02-02-2010.pdf

637-CHE-2004 FORM-2 02-02-2010.pdf

637-che-2004-abstract.pdf

637-che-2004-claims.pdf

637-che-2004-correspondnece-others.pdf

637-che-2004-description(complete).pdf

637-che-2004-description(provisional).pdf

637-che-2004-form 1.pdf

637-che-2004-form 3.pdf

637-che-2004-form 5.pdf

abs-637-che-2004.jpg


Patent Number 246389
Indian Patent Application Number 637/CHE/2004
PG Journal Number 09/2011
Publication Date 04-Mar-2011
Grant Date 25-Feb-2011
Date of Filing 05-Jul-2004
Name of Patentee ORCHID CHEMICALS & PHARMACEUTICALS LTD
Applicant Address ORCHID TOWERS, 313, VALLUVAR KOTTAM HIGH ROAD, NUNGAMBAKKAM, CHENNAI-600 034, INDIA
Inventors:
# Inventor's Name Inventor's Address
1 UDAYAMPALAYAM PALANISAMY SENTHILKUMAR N. UDAYAMPALAYAM POST. KULLAMPALAYAM TQ. GOBICHETTIPALAYAM-638 476 ERODE DIST, TAMILNADU, INDIA
2 VENU SANJEEVI LAKSHMIPATHI PLOT NO.48, II STREET, SHANTHINIKETAN COLONY, MADAMBAKKAM, SELAIYUR CHENNAI-73 TAMILNADU, INDIA
3 ANDREW GNANAPRAKASAM CHOLAN FLATS 27/1, JYOTHI NAGAR 2ND STREET, CHETTLAPAKKAM CHENNAI, TAMILNADU, INDIA
4 CHANDRASEKARAN RAMASUBBU NO.3 (NEW NO.7) B1, RAMMIYAM FOUNDATION, 7TH MAIN ROAD, DHADEESWARNAGAR, VELACHERY, CHENNAI-600 042, TAMILNADU, INDIA
5 DINDIGALA NAGENDER RAO ORCHID CHEMICALS & PHARMACEUTICALS LTD 476/14, SHOLINGANALLUR, OLD MAHABALIPURAM ROAD, CHENNAI-600 119, INDIA
6 GADDAM OM REDDY NO.1, 2ND FLOOR, GOLDEN GLEAMS APARTMENT KARUNANIDHI STREET, KALAKSHETRA COLONY BESANT NAGAR, CHENNAI-600 090 TAMIL NADU, INDIA
PCT International Classification Number C07D 501/00
PCT International Application Number N/A
PCT International Filing date
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 NA