|Title of Invention||
A PROCESS FOR ISOLATING NUCLEOTIDE SEQUENCE FROM THE COLEOPTILE OF GLORIOSA SUPERBA
|Abstract||The present invention relates to a nucleic acid sequence, the product of which, is involved in the metabolism of compounds associated with the development of flavours in the fruit ripening process. The invention also relates to the generation of a nucleic acid sequence, which can be used in the modulation of imparting flavours to fruits.|
Cytochrome P-450 (P-450) monooxygenases are ubiquitous hemoproteins present in microorganisms, plants and animals. Comprised of a large and diverse group of isozymes, P450s mediate a great array of oxidative reactions using a wide range of compounds as substrates, and including biosynthetic processes such as phenylpropanoid, fatty acid, and terpenoid biosynthesis; metaboUsm of natural products; and detoxification of foreign substances (xenobiotics). See e.g., Schuler, Crit. Rev. Plant Sci. 15:235-284 (1996). In a typical P-450 catalyzed reaction, one atom of molecular oxygen (02) is incorporated into the substrate, and the other atom is reduced to water by NADPH. For most eucaryotic P-450s, NADPH:cytochrome P-450 reductase, a membrane-bound flavoprotein, transfers the necessary two electrons from NADPH to the P-450 (Bolwell et al, Phytochemistry 37: 1491-1506 (1994)).
Frear et al. (Phytochemistry 8:2157-2169 (1969)) demonstrated the metabolism of monuron by a mixed-function oxidase located in a microsomal fraction of cotton seedlings. Further evidence has accumulated supporting the involvement of P-450s in the metabolism and detoxification of numerous herbicides representing several distinct classes of compounds (reviewed in Bolwell et al., 1994; Schuler, 1996). Differential herbicide metabolizing P-450 activities are believed to represent one of the mechanisms that enables certain crop species to be more tolerant of a particular herbicide than other crop or weedy species.
1. Total RNA was extracted from ten days old coleoptile tissue.
2. Subsequently, mRNA was isolated from the total RNA.
3. A cDNA hbrary was constructed by making use of the GIBCOBRL Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning Kit.
4. The clones were screened and selected for sequencing and subjected to a BLAST search in order to ascertain their identity.
5. Functional details of the clones were collected and one cDNA clone was designated as cDGsCo04B02, which was predicted to be a homologue of a cell cycle regulator.
1. A nucleic acid gene sequence encoding Cytochrome P-450 isolated from
Terminalia arjuna, possessing the property of modulating the flavours in fruits.
2. A method for producing fruit-bearing plants with enhanced flavour,
comprising expressing a gene encoding Cytochrome P-450 nucleotide
sequence isolated from Terminalia arjuna, in said plant at a level sufficient to
achieve metabolism of compounds associated with the development of
flavours in the fruit ripening process.
3. Transgenic plants expressing cytocrome P-450 nucleotide sequence, thereby
producing fruits with enhanced flavours.
4. A claim as in claim 1, wherein the transgenic plants could apply to all varieties
5. A claim as in claim 1 & 3, wherein the fruits of the transgenic plants
possessing enhanced flavours.
6. A claim as in claim 1, 2 & 3, wherein the product of the gene expression in
said host plant, serves as a useful molecule in the agro and pharma products.
|Indian Patent Application Number||779/CHE/2003|
|PG Journal Number||07/2011|
|Date of Filing||24-Sep-2003|
|Name of Patentee||M/S AVESTHA GENGRAINE TECHNOLOGIES PRIVATE LIMITED|
|Applicant Address||'DISCOVERER' 9TH FLOOR INTERNATIONAL TECH PARK WHITEFIELD ROAD BANGALORE 560 066|
|PCT International Classification Number||A61K35/78|
|PCT International Application Number||N/A|
|PCT International Filing date|