Title of Invention

"AN INDAZOL COMPOUND JNK INHIBITOR"

Abstract An indazol compound JNK inhibitor represented by the formula (III), a salt thereof or a hydrate of them wherein R1 designates a group represented by the formula -CRb=CRc-Ar wherein Rb and Rc each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; Ar designates an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; Rd, Re, Rf, L, X and Y are as herein described.
Full Text The present invention relates to an indazol compound jnk inhibitor.
Technical field
The present invention relates to a novel pyrazole compound having an excellent inhibitory effect on protein kinases, in particular, JNK c-Jun amino-terminal, kinase.
Prior Art
Mitogen-activated protein kinase (hereinafter, referred to as "MAPK") signaling cascades are generally found in from yeast to human, and play a very important role in intracellular signal transduction pathways. As MAPK-related kinases in mammalian cells, in particular, three kinds of kinases are well known: extracellular signal-regulation kinase(ERK), p38 and c-Jun amino-terminal kinase (JNK; or also called as SAPK (=stress-activated protein kinase)). SAPK is a homologue of JNK found in rat, and its isoform group is known to have amino acid sequences of 90% or more homologous to the corresponding isoform group of JNK (Nature, 369, 156, 1994). In recent years, a number of activators involved with MAPK have been identified, which have demonstrated that pathways respectively activating ERK, p38 and JNK have different roles in terms of function. In particular, the JNK pathway is considered to be one of valuable
intracellular signaling pathways from medical and pharmaceutical viewpoints because of the following reasons. JNK is activated, for example, by cytokines such as tumor necrosis factor a (TNF-a) or interleukin-1 (IL-1) , or cell stresses such as heat shock, ultraviolet ray (UV), X-ray and the like, and is considered to be an important signal transduction pathway inducing not only cellular proliferation and differentiation but also apoptosis (cell death) [Science, 270, 1326, 1995.] JNK was initially discovered as a protein which phosphorylates Ser63 and Ser73 located at N-terminal of c-Jun (Nature, 353, 670, 1991), however, at present, JNK is known to phosphorylate a number of transcription factors such as ATF-2 and Elk-1 and regulate their activities (EMBO J., 15, 2760, 1996.) There are three kinds of JNKs: JNK1, JNK2 and JNK3. While JNK1 and JNK2 are expressed in most of tissues, JNK3 is expressed at high level especially inbrain (Neuron, 14, 67, 1995; Neuron, 22, 667, 1999.) Analysis of JNK1 or JNK2 knockout mouse demonstrated that these JNKs have an important role in differentiation and activation of T cell (J. Exp. Med. , 193, 317, 2001) . Also, analysis of JNK1 knockout mouse suggested the importance of JNK1 in onset of metabolic disorders such as insulin resistance caused by obesity and type II diabetes (Nature, 420, 333, 2002). On the other hand, other report described that JNK3 knockout mouse showed resistance against seizure induced by kainic acid which is an excitatory amino acid receptor agonist, and that the apoptosis which would be observed subsequently in hippocampal neurons in normal mouse
was not observed while in JNK3 knockout mouse (Nature 389, 865, 1997.) Since previous research using cultured neurons has proved the necessity of phosphorylation of c-Jun for induction of apoptosisby removal of neurotrophic factor (Neuron 14, 927, 1995; J. Neurosci. 18, 751, 1998), JNK seems to play an important role in inducing apoptosis of neurons . As for neurodegenerative diseases such as Alzheimer disease and Parkinson disease, importance of apoptosis in the process of neurodegeneration has been noticed (Nature 407, 802, 2000), and investigation using model animals of these diseases (Proc Natl Acad Sci USA, 98, 10433, 2001; J. Neurosci. 22, 3376, 2002) and analysis using postmortembrain of patient (Neuron, 14, 67, 1995; J. Neurochem. , 76, 435, 2001) have accumulated the results suggesting the possibility of involvement of JNK in neurodegeneration in Alzheimer's disease and Parkinson's disease.
The following are known reports with regard to substances of low molecular weight having JNK inhibitory effect.
(1) Compounds having anti-inflammatory effect represented by
the formula (I1) and the compound represented by the formula
(Ila) as a concrete embodiment (W000/00491) .
(Formula Removed)
(2) 4-Allyloxyindole compounds represented by the formula (I2)
and the compound represented by the formula (I2a) as a concrete
embodiment (WO00/35909) .
(Formula Removed)
(3) 4,5-Pyrazinoxyindole compounds represented by the formula
(I3) and the compound representedby the formula (I3a) as a concrete
embodiment (WO00/35921).
(Formula Removed)
(4) Compounds represented by the formula (I4) and the compound
represented by the formula (I4a) as a concrete embodiment
(WO00/64872).
(Formula Removed)
(5) Oxyindole derivatives represented by the formula (I5) and
the compound represented by the formula (I5a) as a concrete
embodiment (WO00/35906).
(Formula Removed)
(6) Compounds represented by the formula (I6) having JNK
inhibitory effect and the compound represented by the formula
(I6a) as a concrete embodiment (WO00/75118) .
(Formula Removed)
(7) Compounds represented by the formula (I7) having a JNK
inhibitory effect and the compound represented by the formula
(I7a) as a concrete embodiment (WO01/12609) .
(Formula Removed)
(8) Compounds represented by the formula (I8) having JNK
inhibitory effect and the compound represented by the formula
(I8a) as a concrete embodiment (WO01/12621) .
(Formula Removed)
(9) Sulfonamide derivatives represented by the formula (I9) and
the compound represented by the formulae (I9a) (I9b) (I9C) as
concrete embodiments (WO01/23378, WO01/23379, WO01/23382).
(Formula Removed)
(10) Compounds represented by the formula (I10) having JNK
inhibitory effect and the compound represented by the formula
(I10a) as a concrete embodiment (EP01/110957) .
(Formula Removed)
(11) Compounds represented by the formula (I11) having JNK
inhibitory effect and the compound represented by the formula
(Illa) as a concrete embodiment (WO01/91749) .
(Formula Removed)

On the other hand, compounds having pyrazole skeleton are found only in the report below.
(12) Compounds represented by the formula (I12) having JNK
inhibitory effect and the compound represented by the formula
(I12a) as a concrete embodiment (WO02/10137) .
(Formula Removed)

As described above, JNK pathway is noticed as one of important mechanisms involved in activation of various cells and regulation of immuno cells by cytokines, or in apoptosis of neurons inducedby various kinds of stress signals . Therefore, compounds having inhibitory effect on JNK pathway, especially on JNK protein kinase can be expected to be useful as therapeutic drugs for a variety of immunological diseases, inflammatory diseases, metabolic diseases, neurodegenerative diseases. On the other hand, ERK which belongs to MAPK to which JNK also belongs is known to play an important role in signal transduction of growth factors and neurotrophic factors, and especially in neurons, ERK is deeply concerned with survival and maintenance of neurons accomplished by neurotrophic factors such as Brain-derived neurotrophic factor (BDNF) and the like (Science, 286, 1358, 1999; J. Neurosci., 20, 5775, 2000.) This suggests the possibility of the ERK inhibitory effect to cancel out the useful effect which the compounds having inhibitory effect on
JNK protein kinase are liable to exhibit, e.g., protective effect on neurons, so that a need for discovery of JNK-selective compounds not having ERK inhibitory effect exists. However, such excellent compounds having selective inhibitory effect on JNK protein kinase, while satisfying the points of pharmacological effect, dosage, safety and the like required for pharmaceutics have not been found yet.
.Disclosure of the Invention
As a result of strenuous effort and vigorous research in consideration of the above circumstances, inventors of the present invention finally found a novel pvrazole compound having a JNK inhibitory effect.
That is, the present invention relates to: 1) a compound represented by the formula (I), a salt thereof or a hydrate of the,m: H
(Formula Removed)
(wherein, R1 designates a group represented by the formula
-(CO)h-(NRa)j-(CRb=CRc)k-Ar (wherein Ra, Rb and Rc each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally
substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; Ar designates an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; and h, j and k each independently designate 0 or 1);
Cy designates a 5- to 6-membered heteroaryl group;
V designates a group represented by the formula -L-X-Y (wherein, L designates a single bond, an optionally substituted C1-6 alkylene group, an optionally substituted C2-6 alkenylene group or an optionally substituted C2-6 alkynylene group; X designates a single bond, or a group represented by -NR7-, -O-, -CO-, -S-, -SO-, -SO2-, -CO-NR8-Z-, -C(O)O-, -NR8-CO-Z-, -NR8-C(O)O-, -NR8-S-, -NR8-SO-, -NR8-SO2-Z-, -NR9-CO-NR10-, -NR9-CS-NR10-, -S (O)m-NR11-Z-, -C (=NR12)-NR13-, -0C(O)-, -OC(O)-NR14- or -CH2-NR8-COR7- (wherein R7, R8, R9, R10, R11, R12, R13 and R14 each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl
group, an optionally substituted 4- to 14-membered non-heteroaryl group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group, Z designates a single bond or an optionally substituted C1-6 alkylene group, and in designates 0, 1 or 2); Y designates any one group selected from the group consisting of a hydrogen atom, halogen atom, nitro group, hydroxyl group, cyano group, carboxyl group or an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group, an optionally substituted 5- to 14-membered heteroaryl group, an optionally substituted amino group and a group represented by the formula -W-R15 (wherein W designates CO or SO2; R15 designates an optionally substituted C1-6 alkyl group, an optionally substituted amino group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group)); and
n designates 0, 1, 2, 3 or 4, and when n is 2 or more, plural Vs each independently designate -L-X-Y as defined above);
2) the compound according to 1), a salt thereof or a hydrate of them, wherein Cy forms a 5-membered heteroaryl group;
3) the compound according to 1), a salt thereof or a hydrate
of them, wherein Cy forms a thiophene ring;
4) the compound according to 1), a salt thereof or a hydrate of them, wherein in the formula (I), the structure of moiety consisting of Cy and the pyrazole ring adjoining to the Cy is lH-thieno[2, 3-c]pyrazole;
5) a compound represented by the formula (II), a salt thereof or a hydrate of them:
(Formula Removed)
wherein,
Q1 to Q4 each independently designate -NV1-, -CV2=, -N=, -N(→O)= or -CO-, and at least one of Q1 to Q4 designates -NV1-or -N=, -N(→O)=; and
R1 designates a group represented by the formula -(CO)h- (NRa) J (CRb=CRc)k-Ar (wherein Ra, Rb and Rc each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to
14-membered heteroaryl group; Ar designates an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; and h, j and k each independently designate 0 or 1),
V1 and V2 each independently designate a group represented by the formula -L-X-Y (wherein, L designates a single bond, an optionally substituted C1-6 alkylene group, an optionally substituted C2-6 alkenylene group or an optionally substituted C2-6 alkynylene group;
X designates a single bond, or a group represented by -NR7-, -O-, -CO-, -S-, -SO-, -SO2-, -CO-NR8-Z-, -C(O)O-, -NR8-CO-Z-, -NR8-C(O)O-, -NR8-S-, -NR8-SO-, -NR8-SO2-Z-, -NR9-CO-NR1O-, -NR9-CS~NR10-, -S(O)m-NRn-Z-, -C (=NR12)-NR13-, -OC(O)-, -OC (O)-NR14- or -CH2-NR8-COR7- (wherein R7, R8, R9, R10, R11, R12, R13 and R14 each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group, Z designates a single bond or an optionally substituted C1-6
alkylene group, and m designates 0, 1 or 2); and Y designates any one group selected from the group consisting of a hydrogen atom, halogen atom, nitro group, hydroxyl group, cyano group, carboxyl group or an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group, an optionally substituted 5- to 14-membered heteroaryl group, an optionally substituted amino group and a group represented by the formula -W-R15 (wherein W designates CO or SO2; and R15 designates an optionally substituted C1-6 alkyl group, an optionally substituted amino group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group);
6) the compound according to 5), a salt thereof or a hydrate of them, wherein among Q1 to Q4, either one is -N=, and the others are -CV2=;
7) the compound according to 5), a salt thereof or a hydrate of them, wherein among Q1 to Q4, either one of Q1, Q3 and Q4 is -N=, and the others are -CV2=;
8) the compound according to 6), a salt thereof or a hydrate of them, wherein Q1 is -N=;
9) the compound according to 6), a salt thereof or a hydrate
of them, wherein Q2 is -N=;
10) the compound according to 6) , a salt thereof or a hydrate of them, wherein Q3 is -N=;
11) the compound according to 6) , a salt thereof or a hydrate of them, wherein Q4 is -N=;
12) the compound according to 5) , a salt thereof or a hydrate of them, wherein among Q1 to Q4, either two are -N=, and the others are -CV2=;
13) the compound according to 12), a salt thereof or a hydrate of them, wherein among Q1 to Q4, either two of Qi, Q3 and Q4 are -N=, and the others are -CV2=;
14) the compound according to any one of 5) to 13) , a salt thereof or a hydrate of them, wherein when either of Q1, Q3 and Q4 is -CV2=, the -CV2= in Q1, Q3 or Q4 is -CH=;
15) the compound according to 5) , a salt thereof or a hydrate of them, wherein among Q1 to Q4, either three are -N=, and the other is -CV2=;
16) the compound according to 15) , a salt thereof or a hydrate of them, wherein Q1, Q3 and Q4 are -N=;
17) the compound according to 5) , a salt thereof or a hydrate of them, wherein among Q1 to Q4, at least one is -CO-;
18) the compound according to 5) , a slat thereof or a hydrate of them, wherein Q1 is -CO-, Q2 is -NV1-, and Q3 and Q4 are -CV2=;
19) the compound according to 5) , a slat thereof or a hydrate of them, wherein Q3 is -CO-, Q2 is -NV1-, and Q1 and Q4 are -CV2=;
20) a compound represented by the formula (III) , a salt thereof
or a hydrate of them:
(Formula Removed)
wherein
R1 designates a group represented by the formula -(C0)h-(NRa):-(CRb=CRc)k-Ar (wherein Ra, Rb and Rc each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; Ar designates an optionally substituted C6-i4 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; and h, j and k each independently designate 0 or 1, provided that when h and j are 0, k is 1);
Rd, Re and Rf each independently designate a hydrogen atom, halogen atom, hydroxyl group, cyano group, nitro group, carboxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-7 acyl group, -CO-NR2aR2b, -NR2bCO-R2a or -NR2aR2b (wherein R2a and R2b each
independently designate a hydrogen atom or an optionally substituted C1-6 alkyl group) ;
L designates a single bond, an optionally substituted C1-6 alkylene group, an optionally substituted C2-6 alkenylene group or an optionally substituted C2-6 alkynylene group;
X designates a single bond, or a group represented by -NR7-, -O-, -CO-, -S-, -SO-, -SO2-, -CO-NR8-Z-, -C(O)O-, -NR8-CO-Z-, -NR8-C(O)O-, -NR8-S-, -NR8-SO-, -NR8-SO2-Z-, -NR9-CO-NR10-, -NR9-CS-NR10-, -S (O)m-NRn-Z-, -C (=NR12)-NR13-, -OC(O)-, -OC(O)-NR14- or -CH2-NR8-COR7- (wherein R7, R8, R9, R10, R11, R12, R13 and R14 each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group, Z designates a single bond or an optionally substituted Cl-6 alkylene group, and m designates 0, 1 or 2); and
Y designates any one group selected from the group consisting of a hydrogen atom, halogen atom, nitro group, hydroxyl group, cyano group, carboxyl group or an optionally substituted C1-6
alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group, an optionally substituted 5- to 14-membered heteroaryl group, an optionally substituted amino group and a group represented by the formula -W-R15 (wherein W designates CO or SO2; and R15 designates an optionally substituted C1-6 alkyl group, an optionally substituted amino group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group);
21) the compound according to 20) , a salt thereof or a hydrate of them, wherein at least one of Rd, Re and Rf is not a hydrogen atom;
22) the compound according to 20), a salt threof or a hydrate
of them, wherein either one of Rd, Re and Rf is a halogen atom or an optionally substituted C1-6 alkoxy group;
23) the compound according to any one of 20) to 22) , a salt thereof
or a hydrate of them, wherein at least one of Rb and Rc is not
a hydrogen atom, and L is a single bond, an optionally substituted
C2-6 alkenylene group or an optionally substituted C2-6 alkynylene
group, provided that, when L is a single bond, the case where
X is a single bond, and Y is an optionally substituted C1-6 alkyl
group, an optionally substituted C3-8 cycloalkyl group, an
optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group is excluded;
24) the compound according to any one of 1) to 22) , a salt thereof or a hydrate of them, wherein at least either h or j is 1;
25) the compound according to any one of 1) to 22) , a salt thereof or a hydrate of them, wherein h and j are 0, and k is 1;
26) the compound according to any one of 1) to 19) , a salt thereof or a hydrate of them, wherein h, j and k are 0;

27) the compound according to any one of 24) and 25), a salt thereof or a hydrate of them, wherein Rb and/or Rc are(is) a hydrogen atom;
28) the compound according to 27) , a salt thereof or a hydrate of them, wherein Rb and Rc are a hydrogen atom;
29) the compound according to any one of 1) to 28) , a salt thereof or a hydrate of them, wherein Ar is a C6-14 aryl group or a 5-to 14-membered heteroaryl group, and Ar is a group which may be substituted with 1 to 3 group (s) selected from the following substituent group (a):
the group consisting of (1) each optionally substituted (a) C1-6 alkyl groups, (b) C1-6 alkoxy groups, (c) C1-7 acyl groups, (d) amide group, (e) amino group, (f) C3-8 cycloalkyl groups, (2) halogen atom, (3) hydroxyl group, (4) nitro group, (5) cyano group, and (6) carboxyl group;
30) the compound according to 29) , a salt thereof or a hydrate
of them, wherein Ar is a phenyl group, naphthyl group or a 5-to 10-membered heteroaryl group, and Ar is a group optionally substituted with 1 to 3 group (s) selected from Substituent group a described in 29);
31) the compound according to 29) , a salt thereof or a hydrate of them, wherein Ar is a phenyl group, 2-naphthyl group, pyridyl group, 2-thienyl group, 2-furyl group, 2-benzofuryl group, 2-quinolyl group or 2-benzothienyl group, and Ar is a group optionally substituted with 1 to 3 group(s) selected from Substituent group a described in 29);
32) the compound according to 29) , a salt thereof or a hydrate of them, wherein Ar is a phenyl group, pyridyl group, 2-thienyl group or 2-furyl group, and Ar is a group optionally substituted with 1 to 3 group (s) selected from Substituent group a described in 29);
33) the compound according to 29) , a salt thereof or a hydrate of them, wherein Ar is a 2-naphthyl group, 2-benzofuryl group, 2-quinolyl group or 2-benzothienyl group, and Ar is a group optionally substituted with 1 to 3 group(s) selected from Substituent group a described in 29);
34) the compound according to any one of 29) to 33) , a salt thereof or a hydrate of them, wherein Substituent group a is the group consisting of (1) C1-6 alkyl groups each optionally substituted with 1 to 3 group (s) selected from the group consisting of a halogen atom, hydroxyl group and cyano group, (2) C1-6 alkoxy groups optionally substituted with 1 to 3 group (s) selected from
the group consisting of a halogen atom, hydroxyl group and cyano group, (3) halogen atom, (4) hydroxyl group, (5) cyano group, and (6) C1-7 acyl groups;
35) the compound according to any one of 29) to 33) , a salt thereof or a hydrate of them, wherein Substituent group a is a halogen atom;
36) the compound according to any one of 1) to 35) , a salt thereof or a hydrate of them, wherein L is a single bond or methylene group;
37) the compound according to 36) , a salt thereof or a hydrate of them, wherein L is a single bond;
38) the compound according to any one of 1) to 37) , a salt thereof or a hydrate of them, wherein X is a group represented by -CO-NR8-Z-, -NR8-CO-Z- or -NR8-SO2-Z- (wherein R8 and Z have the same meanings as defined for R8 and Z in 1));
39) the compound according to 38), a salt thereof or a hydrate of them, wherein R8 is a hydrogen atom;
40) the compound according to 38), a salt thereof or a hydrate of them, wherein X is a group represented by -CO-NH-(CH2)t -(wherein t designates 0 or 1);
41) the compound according to 38), a salt thereof or a hydrate of them, wherein X is a group represented by -NH-CO-(CH2) t~ (wherein t designates 0 or 1);
42) the compound according to any one of 1) to 37) , a salt thereof or a hydrate of them, wherein X is a single bond;
43) the compound according to any one of 1) to 42) , a salt thereof
or a hydrate of them, wherein Y is a C1-6 alkyl group, a C6-14 aryl group, a C1-6 alkoxy group, a C3-8 cycloalkyl group, a 4-to 14-membered non-aromatic heterocyclic group or a 5- to 14-membered heteroaryl group, and Y is a group optionally substituted with 1 to 3 group (s) selected from the following Substituent group a2:
the group consisting of (1) each optionally substituted (a) C1-6 alkyl groups, (b) C2-e alkenyl groups, (c) C2-6 alkynyl groups, (d) C1-6 alkoxy groups, (e) C2-i acyl groups, (f) amide group, (g) amino group, (h) C3-8 cycloalkyl groups, (i) C3-8 cycloalkenyl groups, (j) C6-14 aryl groups, (k) 5- to 14-membered heteroaryl groups, (1) C6-14 aryloxy groups, and (m) 4-to 14-membered non-aromatic heterocyclic groups, (2) halogen atom, (3) hydroxyl group, (4) nitro group, (5) cyano group, and (6) carboxyl group;
44) the compound according to 43), a salt thereof or a hydrate of them, wherein Y is a C3-8 cycloalkyl group, phenyl group, a 5- or 6-membered non-aromatic heterocyclic group, or a 5- or 6-membered heteroaryl group, and Y is a group optionally substituted with 1 to 3 group (s) selected from Substituent group a2 described in 43);
45) the compound according to any one of 1) to 42) , a salt thereof or a hydrate of them, wherein Y is a furyl group, thienyl group, pyrrolyl group, phenyl group, pyridyl group, C3-8 cycloalkyl group, tetrahydrofuran-yl group, tetrahydrothiophene-yl group, pyrrolidinyl group, tetrahydrofuran-2-on-yl group,
pyrrolidine-2-on-yl group or a group represented by the formula : (Formula Removed)

(wherein Y2a designates a group represented by -CONH2 or -CH2OH, Y2b and Y2c each independently designate a hydrogen atom, an optionally substitutedphenyl group or an optionally substituted C1-6 alkyl group), and Y is a group optionally substituted with 1 to 3 group(s) selected from Substituent group a2 described in 43);
46) the compound according to 43), a salt thereof or a hydrate of them, wherein Y is a furyl group or thienyl group, and Y is a group optionally substituted with 1 to 3 group (s) selected from Substituent group a2 described in 43);
47) the compound according to any one of 43) to 46), a salt thereof or a hydrate of them, wherein Substituent group a2 is the group consisting of (1) (a) C1-6 alkyl groups, (b) C1-6 alkoxy groups, (C) C1-7 acyl groups, (d) amide group, (e) amino group, (f) C3-8 cycloalkyl groups, each of which may be substituted with 1 to 3 group (s) selected from the following Substituent group b2, (2) halogen atom, (3) hydroxyl group, (4) nitro group, (5) cyano group, and (6) carboxyl group, and is the group consisting of C1-6 alkyl groups, halogen atom, hydroxyl group, nitro group, cyano group and carboxyl group;
48) the compound according to any one of 43) to 46), a salt thereof or a hydrate of them, wherein Substituent group a2 is the group
consisting of (1) C1-6 alkoxy groups, (2) halogen atoms and (3) cyano groups;
49) the compound according to any one of 20) to 35), a salt thereof
or a hydrate of them, wherein L and X are a single bond, Y is
a 5- to 6-membered heteroaryl group, and Y is a group optionally
substituted with 1 to 3 group (s) selected from Substituent group
a2 described in 43);
50) a pharmaceutical composition comprising the compound according to any one of 1) to 49) , a salt thereof or a hydrate of them, and a pharmaceutically acceptable carrier;
51) a c-Jun amino-terminal kinase (JNKs) inhibitor comprising the compound according to any one of 1) to 49), a salt thereof or a hydrate of them;
52) a c-Jun amino-terminal kinase 1 (JNK1), c-Jun amino-terminal kinase 2 (JNK 2) and/or c-Jun amino-terminal kinase 3 (JNK 3) inhibitor, comprising the compound according to any one of 1) to 49), a salt thereof or a hydrate of them;
53) an agent for treating or preventing immunological diseases, inflammatory diseases or metabolic disorders, which comprises the compound according to any one of 1) to 49), a salt thereof or a hydrate of them;

54) an agent for treating or preventing neurodegenerative diseases, which comprises the compound according to any one of 1) to 49), a salt thereof or a hydrate of them;
55) an agent for treating or preventing Alzheimer's disease, Parkinson's disease, Huntington's chorea, amyotrophic lateral
sclerosis, multiple sclerosis or spinocerebellar degeneration, which comprises the compound according to any one of 1) to 49) , a salt thereof or a hydrate of them;
56) use of the compound according to any one of 1) to 49), a salt thereof or a hydrate of them for prevention or treatment of immunological diseases, inflammatory diseases, metabolic disorders and/or neurodegenerative diseases;
57) use of the compound according to any one of 1) to 49), a salt thereof or a hydrate of them, for producing an agent for treating or preventing a disease based on JNK action against which inhibition of a c-Jun amino-terminal kinase (JNK) is effective for prevention or treatment, immunological diseases, inflammatory diseases, metablic disorders or neurodegenerative diseases;

58) the use according to 57) , wherein the disease is Alzheimer' s disease, Parkinson's disease, Huntington' s chorea, amyotrophic lateral sclerosis, multiple sclerosis or spinocerebellar degeneration;
59) a method for treating or preventing a disease based on JNK 3 action against which inhibition of a c-Jun amino-terminal kinase 3 (JNK 3) is effective for prevention or treatment, immunological diseases, inflammatory diseases, metablic disorders and/or neurodegenerative diseases, which comprises adiministering a pharmacologically effective amount of the compound according to any one of 1) to 49), a salt thereof or a hydrate of them to a patient;
60) a method for treating or preventing a disease based on JNK action against which inhibition of a c-Jun amino-terminal kinase (JNK) is effective for prevention or treatment, immunological diseases, inflammatory diseases, metablic disorders or neurodegenerative diseases, which comprises adiministering a pharmacologically effective amount of the compound according to any one of 1) to 49), a salt thereof or a hydrate of them to a patient; and
61) the method according to 60), wherein the disease is Alzheimer's disease, Parkinson's disease, Huntington's chorea, amyotrophic lateral sclerosis, multiple sclerosis or spinocerebellar degeneration.
In the following, definition for symbols, terms and the like used in the present specification will be provided for detailed explanation of the present invention.
The term "and/or" used herein intends to embrace both cases of "and" and "or".
The term "JNK" used herein refers to enzymes that phosphorylates N-terminal region of c-Jun protein, and examples of which include JNK1, JNK2, JNK3 and the like. There are three kinds of JNKs: JNK1, JNK2 and JNK3. While JNK1 and JNK2 are expressed in most of tissues, JNK3 is expressed at high level especially inbrain (Neuron, 14, 67, 1995; Neuron, 22, 667, 1999.)
The term "neurodegenerative diseases" used herein comprehends all diseases that are generally categorized in neurodegenerative diseases in the medical field, and concrete
examples of which include, but not limited to, chronic neurodegenerative diseases such as subarachnoid hemorrhage, cerebrovascular disorder acute stage, head injury, spinal cord injury, neuropathy due to low oxygen and low blood sugar, Alzheimer' s disease, Parkinson's disease, Huntington' s chorea, amyotrophic lateral sclerosis, epilepsy, hepatic encephalopathy, peripheral neuropathy, Parkinson syndrome, exanthematous paralysis, pain, neuralgia, schizophrenia, depression, anxiety, drug dependence, nausea, emesis, urination disorder, visual disorder due to glaucoma, hearing disorder due to antibiotics, alimentary intoxication, multiple sclerosis or spinocerebellar degeneration, and acute neurodegenerative diseases.
The term "immunological diseases" or "inflammatory diseases" usedherein comprehends all diseases that are generally categorized in immunological diseases in the medical field, and concrete examples of which include, but not limited to, sepsis, chronic articular rheumatism, osteoarthritis, gout, psoriasis, psoriatic arthropathy, bronchitis, chronic obstructive pulmonary disease, cyst nature fibroid lung, insulin dependent type I diabetes mellitus, autoimmune thyroiditis, Crohn's disease, colitis ulcerosa, atopic dermatitis, asthma, allergic rhinitis, hepatitis, systemic lupus erythematodes, acute and chronic allograft rejection after organ transplantation, graft-versus-host disease, eczema, hives, myasthenia gravis, acquired immunodeficiency syndrome, idiopathic
thrombocytopenic purpura, glomerulonephritis and the like.
The term "metabolic disorders" used herein refers to diseases caused by metabolic disorder of sugar and lipid, and examples of which include diabetes mellitus, diabetic complication, hypercholesterolemia, hyperlipemia, obesity, syndrome X and the like.
In the present description, a particular structural formula may represent certain isomers, and the present invention comprehends all isomers and mixture of isomers such as geometrical isomers, optical isomers based on an asymmetric carbon, stereoisomers and tautomers occurring due to the particular structure of the compound, but they are not conveniently limited by the description of the above formula, and may be either one isomer or mixture thereof. Therefore, optical isomers and racemic bodies may exist because of an asymmetric carbon in a molecule, however the present invention includes both cases without particularly limited. There is also the case that crystal polymorphs occur, and such crystal polymorphs may exist singly or in mixture without any limitation. The compound (I) or compound (III) or its salt according to the present invention may be anhydride or hydrate, both of which are involved in the scope of claims of the present specification. Metabolites occurring by biodegradation of the compound (I) according to the present invention, and prodrugs of the compound (I) according to the present invention and its salt are also involved in the scope of claims of the present specification.
The "halogen atom" used herein include, for example, a fluorine atom, chlorine atom, bromine atom iodine atom and the like atoms, fluorine atom and chlorine atom are preferred, and fluorine atom is more preferred.
The term "C1-6 alkyl group" used herein refers to linear or branched alkyl groups having 1 to 6 carbon atom (s) , and concrete examples of which include a methyl group, ethyl group, n-propyl group, iso-propyl group, n-butyl group, iso-butyl group, sec-butyl group, tert-butyl group, n-pentyl group, 1,1-dimethylpropyl group, 1,2-dimethylpropyl group, 2,2-dimethylpropyl group, 1-ethylpropyl group, 2-ethylpropyl group, n-hexyl group, l-methyl-2-ethylpropyl group, l-ethyl-2-methylpropyl group, 1,1,2-trimethylpropyl group, 1-propylpropyl group, 1-methylbutyl group, 2-methylbutyl group, 1,1-dimethylbutyl group, 1,2-dimethylbutyl group, 2,2-dimethylbutyl group, 1,3-dimethylbutyl group, 2,3-dimethylbutyl group, 2-ethylbutyl group, 2-methylpentyl group, 3-methylpentyl group and the like, methyl group, ethyl group, n-propyl group, iso-propyl group, .n-butyl group, iso-butyl group, sec-butyl group, tert-butyl group, n-pentyl group and the like are more preferred.
The term "C2-6 alkenyl group" used herein refers to linear or branched alkenyl groups having 2 to 6 carbons, and concrete examples of which include a vinyl group, allyl group, 1-propenyl group, 2-propenyl group, isopropenyl group, 2-methyl-l-propenyl group, 3-methyl-l-propenyl group,
2-methyl-2-propenyl group, 3-methyl-2-propenyl group, 1-butenyl group, 2-butenyl group, 3-butenyl group, 1-pentenyl group, 1-hexenyl group, 1,3-hexadienyl group, 1, 6-hexadienyl group and the like.
The term "C2-6 alkynyl group" used herein refers to linear or branched alkynyl groups having 2 to 6 carbons, and concrete examples of which include an ethynyl group, 1-propynyl group, 2-propynyl group, 1-butynyl group, 2-butynyl group, 3-butynyl group, 3-methyl-l-propynyl group, l-ethynyl-2 propynyl group, 2-methyl-3-propynyl group, 1-pentynyl group, 1-hexynyl group, 1, 3-hexane-diynyl group, 1, 6-hexane-diynyl group, and the like.
The term "C1-6 alkylene group" used herein refers to bivalent groups derived by removing one hydrogen atom at any position from the above defined "C1-6 alkyl group", and concrete examples of which include a methylene group, ethylene group, methylethylene group, propylene group, ethylethylene group, 1,1-dimethylethylene group, 1,2-dimethylethylene group, trimethylene group, 1-methyltrimethylene group, 1-ethyltrimethylene group, 2-methyltrimethylene group, 1,1-dimethyltrimethylene group, tetramethylene group, pentamethylene group, hexamethylene group and the like, with methylene group and 1,2-ethylene group being preferred.
The term "C2-6 alkenylene group" used herein refers to bivalent groups derived by removing one hydrogen atom from the above defined "C2-6 alkenyl group", and concrete examples of which include a vinylene group, propenylene group, butenylene group,pentenylene group, hexenylene group and the like, with vinylene group, propenylene group, butenylene group and pentenylene group being preferred and vinylene group, propenylene group and butenylene group more preferred, and 1,2-vinylene group and 1,3-propenylene group further preferred.
The term "C2-6 alkynylene group" used herein refers to bivalent groups derived by removing one hydrogen atom from the above defined "C2-6 alkynylene group", and concrete examples of which include an ethynylene group, propynylene group, butynylene group, pentynylene group, hexynylene group and the like, ethynylene group, propynylene group, butynylene group and pentynylene group are preferred, ethynylene group, propynylene group and butynylene group are more preferred, ethynylene group and propynylene group are still preferred, and ethynylene group is most preferred.
The term "C3-8 cycloalkyl group" used herein refers to cyclic aliphatic hydrocarbon groups of from 3 to 8 carbons, and concrete examples of which include a cyclopropyl group, cyclobutyl group, cyclopentyl group, cyclohexyl group, cycloheptyl group, cyclooctyl group and the like, and cyclopropyl group and cyclobutyl group are preferred.
The term "C3-8 cycloalkenyl group" used herein refers to C3-8 cycloalkenyl groups having 3 to 8 carbon atoms, and examples of which include cyclopentene-3-yl, cyclohexene-1-yl, cyclohexene-3-yl and the like.
The term "C1-6 alkoxy group" used herein refers to oxy groups
to which the above-defined "C1-6 alkyl group" is bound, and examples of which include methoxy group, ethoxy group, AJ-propoxy group, iso-propoxy group, sec-propoxy group, N-butoxy group, iso-butoxy group, sec-butoxy group, tert-butoxy group, N-pentyloxy group, iso-pentyloxy group, sec-pentyloxy group, N-hexoxy group, iso-hexoxy group, 1,1-dimethylpropyloxy group, 1,2-dimethylpropoxy group, 2,2-dimethylpropyloxy group, 2-ethylpropoxy group, l-methyl-2-ethylpropoxy group, l-ethyl-2-methylpropoxy group, 1,1,2-trimethylpropoxy group, 1,1,2-trimethylpropoxy group, 1,1-dimethylbutoxy group, 1,2-dimethylbutoxy group, 2,2-dimethylbutoxy group, 2,3-dimethylbutoxy group, 1,3-dimethylbutoxy group, 2-ethylbutoxygroup,1,3-dimethylbutoxygroup, 2-methylpentoxy group, 3-methylpentoxy group, hexyloxy group and the like, methoxy group, ethoxy group, n-propoxy group and iso-propoxy group, sec-propoxy group are preferred, and methoxy group and ethoxy group are more preferred.
The term "C2-6 alkenyloxy group" used herein refers to oxy groups to which the above-defined "C2-6 alkenyl group" is bound.
The term "C2-6 alkenylthio group" used herein refers to thio groups to which the above-defined "C2-6 alkenyl group" is bound.
The term "C1-6 alkoxycarbonyl group" used herein refers to carbonyl groups to which the above-defined "C1-6 alkoxy group" is bound, and concrete examples of which include a methoxycarbonylgroup, ethoxycarbonylgroup, n-propoxycarbonyl group, iso-propoxycarbonyl group, n-butoxycarbonyl group,
iso-butoxycarbonyl group, sec-butoxycarbonyl group, tert-butoxycarbonyl group and the like.
The term "C2-7 acyl group" used herein refers to carbonyl groups to which the above-defined "C1-6 alkyl group" is bound, and examples of which include an acetyl group, propionyl group, butylyl group, isobutylyl group, valeryl group, isovaleryl group, pivaloyl group and the like.
Concrete examples of "C1-6 alkylcarbamoyl group" used herein include a methylcarbamoyl group, ethylcarbamoyl group, W-propylcarbamoyl group, iso-propylcarbamoyl group, W-butylcarbamoyl group, iso-butylcarbamoyl group, sec-butylcarbamoyl group, tert-butylcarbamoyl group, N-pentylcarbamoyl group, 1,1-dimethylpropylcarbamoyl group, 1,2-dimethylpropylcarbamoyl group,
2,2-dimethylpropylcarbamoyl group, 1-ethylpropylcarbamoyl group, 2-ethylpropylcarbamoyl group, N-hexylcarbamoyl group, 1-methyl-2-ethylpropylcarbamoyl group, 1-ethyl-2-methylpropylcarbamoyl group, 1,1,2-trimethylpropylcarbamoyl group,
1-propylpropylcarbamoyl group, 1-methylbutylcarbamoyl group, 2-methylbutylcarbamoyl group, 1,1-dimethylbutylcarbamoyl group, 1,2-dimethylbutylcarbamoyl group,
2,2-dimethylbutylcarbamoyl group, 1,3-dimethylbutylcarbamoyl group, 2,3-dimethylbutylcarbamoyl group,
2-ethylbutylcarbamoyl group, 2-methylpentylcarbamoyl group, 3-methylpentylcarbamoyl group and the like.
The term "C1-6 alkylcarbonyloxy group" used herein refers to oxy groups to which the above-defined "C2-7 acyl group" is bound, and concrete examples of which include a methylcarbonyloxy group, ethylcarbonyloxy group, AT-propylcarbonyloxy group, iso-propylcarbonyloxy group, N-butylcarbonyloxy group, iso-butylcarbonyloxy group, sec-butylcarbonyloxy group, tert-butylcarbonyloxy group, N-pentylcarbonyloxy group, 1,1-dimethylpropylcarbonyloxy group, 1,2-dimethylpropylcarbonyloxy group, 2,2-dimethylpropylcarbonyloxy group,
1-ethylpropylcarbonyloxy group, 2-ethylpropylcarbonyloxy group, AKhexylcarbonyloxy group, 1-methyl-2-ethylpropylcarbonyloxy group, l-ethyl-2-methylpropylcarbonyloxy group, 1,1,2-trimethylpropylcarbonyloxy group,
1-propylpropylcarbonyloxy group, 1-methylbutylcarbonyloxy group, 2-methylbutylcarbonyloxy group, 1,1-dimethylbutylcarbonyloxy group, 1,2-dimethylbutylcarbonyloxy group, 2,2-dimethylbutylcarbonyloxy group, 1,3-dimethylbutylcarbonyloxy group,
2,3-dimethylbutylcarbonyloxy group, 2-ethylbutylcarbonyloxy group, 2-methylpentylcarbonyloxy group, 3-methylpentylcarbonyloxy group and the like.
The term "C1-6 alkylsulfonyl group" used herein refers to sulfonyl groups to which the above-defined "C1-6 alkyl group"
is bound, and concrete examples of which include amethylsulfonyl
group, ethylsulfonyl group, N-propylsulfonyl group,
iso-propylsulfonyl group, N-butylsulfonyl group,
iso-butylsulfonyl group, sec-butylsulfonyl group,
tert-butylsulfonyl group, N-pentylsulf onyl group,
1,1-dimethylpropylsulfonyl group, 1,2-dimethylpropylsulfonyl
group, 2,2-dimethylpropylsulfonyl group,
1-ethylpropylsulfonyl group, 2-ethylpropylsulfonyl group,
N-hexylsulfonyl group, l-methyl-2-ethylpropylsulfonyl group,
l-ethyl-2-methylpropylsulfonyl group,
1,1,2-trimethylpropylsulfonyl group, 1-propylpropylsulfonyl
group, 1-methylbutylsulfonyl group, 2-methylbutylsulfonyl
group, 1,1-dimethylbutylsulfonyl group,
1,2-dimethylbutylsulfonyl group, 2,2-dimethylbutylsulfonyl
group, 1,3-dimethylbutylsulfonyl group,
2,3-dimethylbutylsulfonyl group, 2-ethylbutylsulfonyl group,
2-methylpentylsulfonylgroup, 3-methylpentylsulfonylgroup and
the like.
The term "C1-6 alkylsulfinyl group" used herein refers to sulfinyl groups to which the above-defined "C1-6 alkyl group" is bound, and concrete examples of which include a methylsulf inyl group, ethylsulfinyl group, N-propylsulfinyl group, iso-propylsulfinyl group, N-butylsulfinyl group, iso-butylsulfinyl group, sec-butylsulfinyl group, tert-butylsulfinyl group, N-pentylsulfinyl group, 1,1-dimethylpropylsulfinyl group, 1,2-dimethylpropylsulfinyl
group, 2,2-dimethylpropylsulfinyl group,
1-ethylpropylsulfinyl group, 2-ethylpropylsulfinyl group,
N-hexylsulfinyl group, l-methyl-2-ethylpropylsulfinyl group,
1-ethy1-2-methylpropylsulfinyl group,
1,1,2-trimethylpropylsulfinyl group, 1-propylpropylsulfinyl
group, 1-methylbutylsulfinyl group, 2-methylbutylsulfinyl
group, 1,1-dimethylbutylsulfinyl group,
1,2-dimethylbutylsulfinyl group, 2,2-dimethylbutylsulfinyl
group, 1,3-dimethylbutylsulfinyl group,
2, 3-dimethylbutylsulfinyl group, 2-ethylbutylsulfinyl group,
2-methylpentylsulfinylgroup, 3-methylpentylsulfinylgroup and
the like.
The term "C1-6 alkylthio group" used herein refers to thio groups to which the above-defined "C1-6 alkyl group" is bound, and examples of which include a methylthio group, ethylthio group, N-propylthio group, iso-propylthio group, 2V-butylthio group, iso-butylthio group, sec-butylthio group, tert-butylthio group, JV-pentylthio group, 1,1-dimethylpropylthio group, 1,2-dimethylpropylthio group, 2,2-dimethylpropylthio group, 1-ethylpropylthio group, 2-ethylpropylthio group, N-hexylthio group, l-methyl-2-ethylpropylthio group,
l-ethyl-2-methylpropylthio group, 1,1,2-trimethylpropylthio group, 1-propylpropylthio group, 1-methylbutylthio group, 2-methylbutylthio group, 1,1-dimethylbutylthio group, 1,2-dimethylbutylthio group, 2,2-dimethylbutylthio group, 1,3-dimethylbutylthio group, 2,3-dimethylbutylthio group,
2-ethylbutylthio group, 2-methylpentylthio group, 3-methylpentylthio group and the like.
The term "C6-14 aryl group" used herein refers to aryl groups of from 6 to 14 carbon atoms, which include a monocyclic group and condensed rings such as bicyclic group and tricyclic group. Concrete examples of such group include a phenyl group, indanyl group, 1-naphthyl group, 2-naphthyl group, azulenyl group, heptalenyl group, biphenyl group, indacenyl group, acenaphthyl group, fluorenyl group, phenalenyl group, phenanthrenyl group, anthracenyl group, cyclopentacyclooctenyl group, benzocyclooctenyl group and the like. In the wC6-14 aryl group", phenyl group, 1-naphthyl group or 2-naphthyl group is preferred, and phenyl group, indanyl group or 2-naphthyl group is more preferred.
The term "C6-14 aryloxy group" used herein refers to oxy groups to which the above-defined "C6-14 aryl group" is bound.
The term "5- to 14-membered heteroaryl group" used herein refers to monocyclic, bicyclic or tricyclic, 5- to 14-membered heteroaryl groups containing one or more hetero atom selected from the group consisting of a nitrogen atom, sulfur atom and oxygen atom. Concrete examples of such group include 1) nitrogen-containing heteroaryl groups such as pyrrolyl group, pyridyl group, pyridazinyl group, pyrimidinyl group, pyrazinyl group, triazolylgroup, tetrazolylgroup,benzotriazolylgroup, pyrazolyl group, imidazolyl group, benzimidazolyl group, indolyl group, isoindolyl group, indolizinyl group, purinyl
group, indazolyl group , quinolyl group, isoquinolyl group, quinolizinyl group, phthalazyl group, naphthyridinyl group, quinoxalyl group, quinazolinyl group, cinnolinyl group, pteridinyl group, imidazotriazinyl group, pyrazino pyridazinyl group, acridinylgroup, phenanthridinyl group, carbazolylgroup, carbazolinyl group, pyrimidinyl group, phenanthrolinyl group, phenacynyl group, imidazopyridinyl group, imidazopyrimidinyl group, pyrazolopyridinyl group, pyrazolopyridinyl group and the like; 2) sulfur-containing heteroaryl groups such as thienyl group, benzothienyl group and the like; 3) oxygen-containing heteroaryl groups such as furyl group, pyranyl group, benzofuryl group, isobenzofuryl group and the like; 4) heteroaryl groups containing two or more different hetero atoms such as thiazolyl group, isothiazolyl group, benzothiazolyl group, benzthiadiazolyl group, phenothiazinyl group, isoxazolyl group, furazanyl group, phenoxazinyl group, oxazolyl group, isoxazoyl group, benzoxazolyl group, oxadiazolyl group, pyrazolooxazolyl group, imidazothiazolyl group, thienofuranyl group, furopyrrolyl group, pyridoxadinyl group and the like.
The term "4- to 14-membered non-aromatic heterocyclic group" used herein refers to non-aromatic heterocyclic groups having the following features:
1) the number of atoms constituting the ring of the cyclic group is from 4 to 14;
2) at least one hetero atom is contained in the atoms constituting the ring of the cyclic group;
3) the ring may contain 1 to 3 carbonyl group(s);
4) monocyclic, bicyclic or tricyclic . Concrete examples of such
group include pyrrolidyl group, pyrrolyl group, piperidyl group,
pyperazyl group, imidazolyl group, pyrazolidyl group,
imidazolidyl group, morphoryl group, tetrahydrofuryl group,
tetrahydropyranyl group, aziridinyl group, oxylanyl group,
oxathiolanyl group and the like. Such non-aromatic
heterocyclic groups also include groups derived from pyridine
ringandnonaromatic condensedrings (forexample, groups derived
from phthalimide ring, succinimide ring or the like), and
pyrrolidyl group, pyrrolyl group, piperidyl group, pyperazyl
group, imidazolyl group, pyrazolidyl group, imidazolidyl group,
morphoryl group, tetrahydrofuryl group, tetrahydropyranyl
group, aziridinyl group, oxylanyl group, oxathiolanyl group and
the like are preferred.
The term "5- to 10-membered heteroaryl group" used herein refers to monocyclic or bicyclic heteroaryl groups in which the ring of the cyclic group is made up of 5 to 10 atoms and at least one hetero atom (s) is (are) contained in the atoms constituting the ring of the cyclic group. Examples of such group include 1) nitrogen-containing heteroaryl groups such as pyrrolyl group, pyridyl group, pyridazinyl group, pyrimidinyl group, pyrazinyl group, triazolylgroup, tetrazolylgroup,benzotriazolylgroup, pyrazolyl group, imidazolyl group, benzimidazolyl group, indolyl group, isoindolyl group, indolizinyl group, purinyl group, indazolyl group, quinolyl group, isoquinolyl group,
quinolizyl group, phthalazyl group, naphthyridinyl group, quinoxalyl group, quinazolinyl group, cinnolinyl group, pteridinyl group, imidazotriazinyl group, pyrazino pyridazinyl group, imidazopyridinyl group, imidazopyrimidinyl group, pyrazolopyridinyl group, pyrazolopyridinyl group and the like; 2) sulfur-containing heteroaryl groups such as thienyl group,benzothienyl group and the like; 3) oxygen-containing heteroaryl groups such as furyl group, pyranyl group, benzofuryl group, isobenzofuryl group and the like; 4) heteroaryl groups containing two or more different hetero atoms such as thiazolyl group, isothiazolyl group, benzothiazolyl group, benzthiadiazolyl group, isoxazolyl group, furazanyl group, oxazolyl group, isoxazoyl group, benzoxazolyl group, oxadiazolyl group, pyrazolooxazolyl group, imidazothiazolyl group, thienofuranyl group, furopyrrolyl group, pyridoxadinyl group and the like.
Among these, pyrrolyl group, furyl group, thienyl group, pyridyl group, benzothienyl group, benzofuryl group, indolyl group, benzlyl group and indazolyl group are preferred, and furyl group, thienyl group, benzothienyl group and benzofuryl group are more preferred.
The term "5- to 6-membered heteroaryl" used herein refers to monocyclic heteroaryl group in which the ring of the cyclic group is made up of 5 to 6 atoms and at least one hetero atom(s) is (are) contained in the atoms constituting the ring of the cyclic group. Examples of such group include pyrrolyl group,
imidazolyl group, pyrazolyl group, 1,2,3-triazolyl group, pyridyl group, pyridazyl group and pyrimidinyl group, pyrazinyl group, triazinyl group, furyl group, thienyl group, thiazolyl group, oxazolyl group, isooxazolyl group and the like, and "heteroaryl" used herein contains an optionally substituted pyridonyl group on a nitrogen atom. Among these, pyrrolyl group, pyridyl group, piridonyl group, pyridazinyl group, pyrimidinyl group, pyrazinyl group, triazinyl group, furyl group or thienyl group is preferred.
The term "5-membered heteroaryl" used herein refers to 5-membered heteroaryl groups containing at least one heteroatom selected from the group consisting of a nitrogen atom, sulfur atom and oxygen atom. Examples of such group include pyrrolyl group, imidazolylgroup,pyrazolylgroup,1,2,3-triazolylgroup, furyl group, thienyl group, oxazolyl group, isooxazolyl group and the like, furyl group or thienyl group being preferred, and thienyl group more preferred.
The term "5- to 6-membered non-aromatic heterocycle" used herein refers to 5- or 6-membered heterocyclic groups containing at least one heteroatom selected from the group consisting of a nitrogen atom, sulphur atomand oxygen atom. Concrete examples of such group include piperidyl group, piperazyl group, morpholyl group, thiomorpholyl group, tetrahydro-2-pyron-yl group, tetrahydropyran-yl group, tetrahydrothiopyran-yl group, pyperidine-2-on-yl group, tetrahydrofuran-yl group, tetrahydrothiophene-yl group, pyrrolidinyl group,
tetrahydrofuran-2-on-yl group or pyrrolidine-2-on-yl group. In the above "5- or 6-membered non-aromatic heterocycle", piperidyl group, piperazyl group, morpholyl group, thiomorpholyl group, tetrahydro-2-pyron-yl group, tetrahydropyran-yl group, tetrahydrothiopyran-yl group and pyperidine-2-on-yl group are preferred.
The term "amino group" used herein comprehends primary amines represented by the formula -NH2, as well as secondary amines in which a hydrogen atom is substituted with other substituent (for example, C1-6 alkyl group and the like) and tertiary amines in which two hydrogen atoms are substituted . In the cases of tertiary amines, two substituents may be bound with each other, to form a A- to 8-membered ring (for example, piperidine ring, piperazine ring, morpholine ring or the like) .
The term "amide group" used herein comprehends groups represented by -CO-NH2, as well as secondary or tertiary amides in which hydrogen atom is substituted with C1-6 alkyl group or the like as described in the definition for "amino group". The term "amide group" also involves amide groups forming a ring such as lactam.
The term "furyl group" used herein refers to 2-furyl group or 3-furyl group, with 2-furyl group being preferred.
The term "thienyl group" used herein refers to 2-thienyl group or 3-thienyl group, with 2-thienyl group being preferred.
The term "pyrrolyl group" used herein refers to 1-pyrrolyl group, 2-pyrrolyl group or 3-pyrrolyl group, with 2-pyrrolyl
group being preferred.
The term "tetrahydrofuran-yl group" used herein refers to tetrahydrofuran-2-yl group or tetrahydrofuran-3-yl group, with tetrahydrofuran-2-yl group being preferred.
The term "tetrahydrothiophene-yl group" used herein refers to tetrahydrothiophene-2-yl group or tetrahydrothiophene-3-yl group, with tetrahydrothiophene-2-yl group being preferred.
The term "pyrrolidinyl group" used herein refers to 1-pyrrolidinyl group, 2-pyrrolidinyl group or 3-pyrrolidinyl group, with 2-pyrrolidinyl group being preferred.
The term "tetrahydrofuran-2-on-yl group" used herein refers to tetrahydrofuran-2-on-3-yl group,
tetrahydrofuran-2-on-4-yl group or tetrahydrofuran-2-on-5-yl group, with tetrahydrofuran-2-on-5-yl group being preferred.
The term "pyrrolidine-2-on-yl group" used herein refers to pyrrolidine-2-on-l-yl group, pyrrolidine-2-on-3-yl group, pyrrolidine-2-on-4-yl group or pyrrolidine-2-on-5-yl group, and pyrrolidine-2-on-5-yl group is preferred.
The term "quinolyl group" used herein refers to monovalent groups derived by removing any one of hydrogen atoms from a quinoline ring, and concrete examples of which include 2-quinolyl group, 3-quinolyl group, 4-quinolyl group, 5-quinolyl group, 6-quinolyl group, 7-quinolyl group and 8-quinolyl group, and 2-quinolyl group is preferred.
In the groups represented by the formula:

(Formula Removed)
(wherein Y2a, Y2b and Y2C have the same meaning as defined above) ,
preferred examples include the groups represented by the formula: (Formula Removed)

The term "optionally substituted" used herein is synonymous
with the expression "a site at which substitution is possible may have one or plural substituent (s) in arbitrary combination" . Typical examples of substituent involved in "optionally substituted" include:
(1) halogen atoms;
(for example, fluorine atom, chlorine atom, bromine atom, iodine atom and the like);
(2) hydroxy1 groups;
(3) cyano groups;
(4) nitro groups;
(5) carboxyl groups;
(6) amino groups;
(7) C1-6 alkyl groups
(for example, methyl group, ethyl group, A7-propyl group, iso-propyl group, N-butyl group, tert-butyl group, iV-pentyl group, 1,1-dimethylpropyl group, 1,2-dimethylpropyl group, 2,2-dimethylpropyl group, 1-ethylpropyl group, 2-ethylpropyl
group, N-hexyl group and the like);
(8) C2-6 alkenyl groups
(for example, vinyl group, allyl group, 1-propenyl group, 2-propenyl group, isopropenyl group, 2-methyl-l-propenyl group, 3-methyl-l-propenyl group and the like);
(9) C2-6 alkynyl groups
(for example, ethynylgroup, 1-propynylgroup, 2-propynylgroup, 1-butynyl group, 2-butynyl group, 3-butynyl group, 3-methyl-l-propynyl group, l-ethynyl-2propynyl group, 2-methyl-3-propynyl group and the like);
(10) C3-8 cycloalkyl groups
(for example, cyclopropyl group, cyclobutyl group, cyclopentyl group, cyclohexyl group, cycloheptyl group, cyclooctyl group and the like);
(11) C3-8 cycloalkenyl groups
(for example, cyclopropene-1-yl, cyclopropene-3-yl,
cyclobutene-1-yl, cyclobutene-3-yl, 1,3-cyclobutadiene-l-yl,
cyclopentene-1-yl, cyclopentene-3-yl, cyclopentene-4-yl,
1,3-cyclopentadiene-l-yl, 1,3-cyclopentadiene-2-yl,
1,3-cyclopentadiene-5-yl, cyclohexene-1-yl, cyclohexene-3-yl,
cyclohexene-4-yl, 1,3-cyclohexadiene-l-yl,
1,3-cyclohexadiene-2-yl, 1,3-cyclohexadiene-5-yl,
1,4-cyclohexadiene-3-yl, 1,4-cyclohexadiene-l-yl and the
like);
(12) C1-6 alkoxy groups
(for example, methoxy group, ethoxy group, iV-propoxy group,
iso-propoxy group, sec-propoxy group, N-butoxy group, iso-butoxy group, sec-butoxy group, tert-butoxy group, N-pentyloxy group, iso-pentyloxy group, sec-pentyloxy group, N-hexoxy group, iso-hexoxy group, 1,1-dimethylpropyloxy group, 1, 2-dimethylpropoxy group, 2, 2-dimethylpropyloxy group and the like);
(13) C1-6 alkenyloxy groups
(for example, vinyloxy group, allyloxy group, 1-propenyloxy group, 2-propenyloxy group, isopropenyloxy group, 2-methyl-l-propenyloxy group, 3-methyl-l-propenyloxy group, 2-methyl-2-propenyloxy group, 3-methyl-2-propenyloxy group, 1-butenyloxy group, 2-butenyloxy group, 3-butenyloxy group, 1-pentenyloxy group, 1-hexenyloxy group, 1,3-hexadienyloxy group, 1,6-hexadienyloxy group and the like);
(14) C1-6 alkylthio groups
(for example, methylthio group, ethylthio group, N-propylthio group, iso-propylthio group, N-butylthio group, iso-butylthio group, sec-butylthio group, tert-butylthio group, N-pentylthio group, 1,1-dimethylpropylthio group, 1,2-dimethylpropylthio group, 2, 2-dimethylpropylthio group, 1-ethylpropylthio group, 2-ethylpropylthio group, iV-hexylthio group, l-methyl-2-ethylpropylthio group, and the like);
(15) C1-6 alkenylthio groups
(for example, vinylthio group, allylthio group, 1-propenylthio group, 2-propenylthio group, isopropenylthio group, 2-methyl-l-propenylthio group, 3-methyl-l-propenylthio group,
2-methyl-2-propenylthio group, 3-methyl-2-propenylthio group, 1-butenylthio group, 2-butenylthio group, 3-butenylthio group, 1-pentenylthio group, 1-hexenylthio group, 1,3-hexane dienylthio group, 1, 6- hexane dienylthio group, and the like) ;
(16) C1-14 aryloxy groups
(for example, phenyloxy group and the like);
(17) C2-7 acyl groups
(for example, acetyl group, propionyl group, butyroyl group and the like);
(18) C6-14 aromatic hydrocarbon cyclic groups
(for example, phenyl group, 1-naphthyl group, 2-naphthyl group, and the like);
(19) 4- to 14-membered non-aromatic hydrocarbon cyclic groups
(for example, 1) pyrrolidyl group, pyrrolilyl group, piperidyl
group, pyperazyl group, imidazolyl group, pyrazolidyl group,
imidazolidyl group, morphoryl group, tetrahydrofuryl group,
tetrahydropyranyl group, aziridinyl group, oxylanyl group and
oxathiolanyl group;
2) groups derived from pyrrolidone ring;
3) groups derived from condensed rings such as phthalimide ring
and succinimide group, and the like);
(20) 5- to 14-membered aromatic heterocyclic groups
(for example, pyrrolyl group, pyridyl group, pyridazinyl group, pyrimidyl group, pyrazinyl group, imidazolyl group, benzimidazolyl group, indolyl group, indazolyl group, quinolyl group, isoquinolyl group, thienyl group, benzothienyl group,
furyl group, pyranyl group, benzofuryl group, thiazolyl group, benzothiazolyl group and the like);
(21) amide group,
(22) sulfonyl groups having C1-6 aliphatic hydrocarbon groups as substituent;
(23) sulfonamide groups,
(24) C1-6 alkylcarbamoyl groups,
(25) C1-6 alkoxycarbonyl groups,
(26) C1-6 alkylcarbonyloxy groups,
(27) C1-6 alkylsulfonyl groups,
(28) C1-6 alkylsulfinyl groups,
(29) formyl group,
(30) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group)
(31) the formula:
(Formula Removed)

(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group)
(32) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group)
and the like groups, and the term "optionally substituted" used herein means that the compound may have 1 to 4 substituent (s) selected from the above substituent groups.
In the above (6) to (23) listing available substituents for "optionally substituted", amino group, C1-6 alkyl groups, C2-6 alkenyl groups, C2-e alkynyl groups, C3-8 cycloalkyl groups, C3-8 cycloalkenyl groups, C1-6alkoxy groups, C1-6alkenyloxy groups, C1-6 alkylthio groups, C1-6 alkenylthio groups, C1-14 allyloxy groups, C2-7 acyl groups, C6-14 aryl groups, 4- to 14-membered non-aromatic hydrocarbon cyclic groups or 5- to 14-membered heteroaryl groups, amide groups, sulfonyl groups having C1-6 aliphatic hydrocarbon groups as substituent or sulfonamide groups may be further optionally substituted with 1 to 4 group (s) selected from the group consisting of:
(a) halogen atoms,
(b) hydroxyl group, (C) cyano group,

(d) nitro group,
(e) carboxyl group,
(f) amino group,
(g) C1-6 alkyl groups,
(h) C2-6 alkenyl groups,
(i) C2-6 alkynyl groups,
(j) C3-8 cycloalkyl groups,
(k) C3-8 cycloalkenyl groups,
(1) C1-6 alkoxy groups,
(m) C1-6 alkenyloxy groups,
(n) C1-6 alkylthio groups,
(o) C1-6 alkenylthio groups,
(p) C1-14 allyloxy groups,
(q) C2-7 acyl groups,
(r) C6-14 aryl groups,
(s) 4- to 14-membered non-aromatic hydrocarbon cyclic groups,
(t) 5- to 14-membered heteroaryl groups,
(u) amide group,
(v) sulfonyl groups having C1-6aliphatic hydrocarbon groups as substituent, and
(w) sulfonamide groups
as described in (1) to (23).
Preferred examples of substituents for "optionally substituted" include:
(a-1) halogen atoms,
(a-2) hydroxyl group,
(a-3) nitrile group,
(a-4) C1-6 alkyl groups, C2-6 alkenyl groups, C2-6 alkynyl groups, C3-8 cycloalkyl groups and C1-6 alkoxy groups each optionally substituted with 1 to 3 halogen atom(s) or hydroxyl group,
(a-5) C6-10 aryl groups.
(a-6) 5- to 14-membered heteroaryl groups,
(a-7) 5- to 14-membered heterocyclic groups,
(a-8) carboxyl group,
(a-9) trifluoromethyl group,
(a-10) C1-6 alkylcarbamoyl groups,
(a-11) C1-6 alkoxycarbonyl groups,
(a-12) C2-7 acyl groups,
(a-13) C1-6 alkylcarbonyloxy groups,
(a-14) C1-6 alkylsulfonyl groups,
(a-15) C1-6 alkylsulfinyl groups,
(a-16) C1-6 alkylthio groups,
(a-17) nitro group,
(a-18) formyl group,
(a-19) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group) , (a-20) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group) ,
(a-21) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group) and the like substituents.
More preferred examples of substituents for "optionally substituted" include: (a-1) halogen atom, (a-2) hydroxyl group, (a-3) nitrile group,
(a-4) C1-6 alkyl groups, C3-8 cycloalkyl groups and C1-6 alkoxy groups each optionally substituted with 1 to 3 halogen atom(s) or hydroxyl group, (a-17) nitro group, and the groups represented by (a-19) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen atom or C1-6 alkyl group) , and (a-20) the formula:
(Formula Removed)
(wherein R10a and Rlla each independently designate a hydrogen
atom or C1-6 alkyl group) .
More preferred examples of substituents for "optionally substituted" include halogen atoms, nitrile group, C1-6 alkyl groups, C3-8 cycloalkyl groups, C1-6 alkoxy groups and trifluoromethyl groups.
More preferred examples of substituents for "optionally substituted" include a fluorine atom, cyclopropyl group, trifluoromethyl group, methoxy group or the like.
When the number of substituent is 2 or more in "optionally substituted" used herein, the substituents may be bound with each other to form a ring. For example, when referring to "optionally substituted phenyl group", piperonyl group and the like are included.
In the compounds representedby the formula (I) in accordance with the present invention, Cy is 5- to 6-membered heteroaryl, preferably 5-membered heteroaryl, more preferably a thiophene ring, and most preferably such that structure of a moiety consisting of the Cy and the pyrazole ring adjoining the Cy forms
lH-thieno [2,3-C] pyrazole.
In the compounds represented by the formula (I) according to the present invention, n is 0, 1, 2, 3 or 4, and preferably 1 or 2.
In the compounds represented by the formula (II), Q1 to Q4 each independently designate -NV1-, -CV2=, -N=, -N (→())= or -CO-, and at least one of Q1 to Q4 is -NV1- or -N=, -N(-^0)=, namely containing a nitrogen atom on the ring, and it is preferred
that only one of Q1 to Q4 is -NV1- or -N=, -N(-»0)=, and -N= is more preferable in the substituents of -NV1- or -N=, -N (→O)=. Furthermore, it is preferred that either one of Q1, Q3 or Q4 is -NV1- or -N=, -N(→O)=, especially -N=.
In the compounds represented by the formula (III) according to the present invention, Rd, Re or Rf each independently designate a hydrogen atom, halogen atom, hydroxyl group, cyano group, nitro group, carboxyl group, optionally substituted C1-6 alkyl group, optionally substituted C1-6 alkoxy group, optionally substituted C2-7 acyl group, -CO-NR2aR2b, -NR2bCO-R2a or -NR2aR2b (wherein R2a and R2b each independently designate a hydrogen atom or an optionally substituted C1-6 alkyl group), and it is preferable that at least one of Rd, Re and Rf is not a hydrogen atom, and only one of Rd, Re and Rf is not a hydrogen atom. That is at least one or more of Rd, Re and Rf is (are) preferably a substituent other than hydrogen atom, and it is more preferred that either two of Rd, Re and Rf are hydrogen atoms while the other one is a substituent other than hydrogen atom. When expresses as "other than hydrogen atom", a halogen atom, hydroxyl group or optionally substituted alkoxy group is a preferred substituent, and a fluorine atom or methoxy group is a more preferred substituent.
In the compounds represented by the formulae (I) to (III) according to the present invention, R1 is a group represented by the formula - (CO)h- (NRa)J (CRb=CRc) k-Ar (wherein Ra, Rb and Rc each independently designate a hydrogen atom, halogen atom,
hydroxyl group, optionally substituted C1-6 alkyl group, optionally substituted C2-6 alkenyl group, optionally substituted C1-6 alkoxy group, optionally substituted C2-6 alkenyloxy group, optionally substituted C1-6 alkylthio group, optionally substituted C2-6 alkenylthio group, optionally substituted C3-8 cycloalkenyl group, optionally substituted 4-to 14-membered non-aromatic heterocyclic group, optionally substituted C6-14 aryl group or optionally substituted 5- to 14-membered heteroaryl group, Ar designates an optionally substituted C6-14 aryl group or optionally substituted 5- to 14-membered heteroaryl group, h, j and k each independently designate 0 or 1) , and it is preferred that h and j are 0, and it is more preferred that h and j are 0 and k is 1.
In the compounds represented by the formulae (I) to (III) according to the present invention, Ra, Rb and Rc each independently designate a hydrogen atom, halogen atom, hydroxyl group, optionally substituted C1-6 alkyl group, optionally substituted C2-6 alkenyl group, optionally substituted C1-6 alkoxy group, optionally substituted C2-6 alkenyloxy group, optionally substituted C1-6 alkylthio group, optionally substituted C2-6 alkenylthio group, optionally substituted C3_8 cycloalkenyl group, optionally substituted 4- to 14-membered non-aromatic heterocyclic group, optionally substituted C6-14 aryl group or optionally substituted 5- to 14-membered heteroaryl group, preferably a hydrogen atom, halogen atom, hydroxyl group, optionally substituted C1-6 alkyl group, optionally substituted
C2-6 alkenyl group or optionally substituted C1-6 alkoxy group, and more preferably a hydrogen atom or halogen atom.
In the compound represented by the formulae (I) to (III) according to the present invention, Ar is an optionally substituted C6-14 aryl group or optionally substituted 5- to 14-membered heteroaryl group, preferably an optionally substituted benzene ring, optionally substituted naphthalene ring, optionally substituted thiophene ring or optionally substituted pyridine group.
In the compounds represented by formulae (I) to (III) according to the present invention, L is a single bond, optionally substituted C1-6 alkylene group, optionally substituted C2-6 alkenylene group or optionally substituted C2-6 alkynylene group, preferably a single bond or optionally substituted C1-6 alkylene group, and more preferably a single bond, methylene group or ethylene group.
In the compounds represented by the formulae (I) to (III) according to the present invention, X designates a single bond or a group represented by -NR7-, -O-, -CO-, -S-, -SO-, -SO2-, -CO-NR8-Z-, -C(O)O-, -NR8-CO-Z-, -NR8-C(O)O-, -NR8-S-, -NR8-SO-, -NR8-SO2-Z-, -NR9-CO-NR10-, -NR9-CS-NR10-, -S (O) m-NRn-Z-, -C(=NR12)-NR13-, -OC(O)-, -OC(O)-NR14-or-CH2-NR8-COR7- (wherein R7, R8, R9, R10, R11, R12, R13 and R14 each independently designate a hydrogen atom, halogen atom, hydroxyl group, optionally substituted C1-6 alkyl group, optionally substituted C2-6 alkenyl group, optionally substituted C2-6 alkynyl group, optionally
substituted C1-6 alkoxy group, optionally substituted C2-6 alkenyloxy group, optionally substituted C1-6 alkylthio group, optionally substituted C2-6 alkenylthio group, optionally substituted C3-8 cycloalkyl group, optionally substituted C3-8 cycloalkenyl group, optionally substituted 4- to 14-membered non-aromatic heterocyclic group, optionally substituted C6-14 aryl groupor optionally substituted 5- to 14-memberedheteroaryl group, Z designates a single bond or optionally substituted C1-6 alkylene group, m designates 0, 1 or 2) , and preferably a single bond, -CO-NR8-Z-, or -NR8-CO-Z-.
In the compounds represented by the formulae (I) to (III) according to the present invention, Y is selected from the group consisting of a hydrogen atom, halogen atom, nitro group, hydroxyl group, cyano group, carboxyl group or optionally substituted C1-6 alkyl group, optionally substituted C2-6 alkenyl group, optionally substituted C2~e alkynyl group, optionally substituted Cl-6 alkoxy group, optionally substituted C3-8 cycloalkyl group, optionally substituted C3-8 cycloalkenyl group, optionally substituted 4- to 14-membered non-aromatic heterocyclic group, optionally substituted C6-14 aryl group, optionally substituted 5- to 14-membered heteroaryl group, optionally substituted amino group and group represented by the formula -W-R15 wherein W designates CO or SO2; R15 designates an optionally substituted C1-6 alkyl group, optionally substituted amino group, optionally substituted C6-14 aryl group or optionally substituted 5- to 14-membered heteroaryl group) ,
preferably an optionally substituted 5- to 14-membered heteroaryl group, andmorepref erably 5- to 6-memberedheteroaryl group. Furthermore, when Y is -W-R15, W is CO, while when R15 is optionally substituted C1-6 alkyl group, preferred is an optionally substituted amino group.
In the compounds represented by the formulae (I) to (III) according to the present invention, R7, R8, R9, R10, R11, R12, R13 and R14 each independently designate a hydrogen atom, halogen atom, hydroxyl group, optionally substituted C1-6 alkyl group, optionally substituted C2-6 alkenyl group, optionally substituted C2-6 alkynyl group, optionally substituted C1-6alkoxy group, optionally substituted C2-6 alkenyloxy group, optionally substituted C1-6 alkylthio group, optionally substituted C2-6 alkenylthio group, optionally substituted C3-8 cycloalkyl group, optionally substituted C3-8 cycloalkenyl group, optionally substituted 4- to 14-membered non-aromatic heterocyclic group, optionally substituted C6-14 aryl group or optionally substituted 5- to 14-membered heteroaryl group, and preferably hydrogen atom or optionally substituted C1-6 alkyl group.
Now, general methods for synthesizing the compounds represented by the formulae (I) to (III) according to the present invention will be described. It goes without saying that in the following general synthesis methods, exemplification based on the above formula (I) also applies to the formulae (II) and (III) unless otherwise noticed. Inparticular, as for themethod of introducing the substituent R1 as described later, it obviously
applies to the formulae (II) and (III).
(Formula Removed)
A typical production method of condensed pyrazole compound
represented by the formula (I) according to the present invention
will be described below. It is to be noted that "Cy" used in
the reaction schemes of Production methods 1 to 80 has the same
meaning as defined above. R1, Q1, Q2, Q3, Q4 and V each have the
same meaning as defined above. "V" indicated as a reagent in
schemes of the Production methods provided below may exists alone
or associated with an appropriate leaving group. The symbol
"n" designates 0, 1, 2, 3 or 4 . T1 means a hydrogen atom, bromine
atomor iodine atom. T2means a halogen atom, preferably fluorine
atom. T3 means a chlorine atom, bromine atom or iodine atom,
preferably bromine atom or iodine atom. Pro and Pro1 each
designate a protective group. J, J1 and J2 each independently
designate, but not limited to, an alkyl group or may together
form a ring. V1 and V2 each have the same meaning as defined
for V.
Production method 1
(Formula Removed)
Compound (I) can be produced by converting aromatic ring compound 1 into a metal aryl with the use of alkyl lithium, lithium amide or the like, reacting the metal aryl with aryl aldehyde to render it alcohol 2, oxidizing alcohol 2 into ketone 3, and then subjecting ketone 3 to cyclization of indazole ring with the use of hydrazine. As the alkyl lithium to be used for converting aromatic ring compound 1 into ametal aryl, for example, W-butyllithium, sec-butyllithium, tert-butyllithium, phenyl lithium and the like are used. Additives such as diazabicyclo[2.2.0]octane, N,N,N' ,N'-tetramethylethylenediamine,
hexamethylphosphoramide and the like may also be added as is necessary. Further, as the lithium amide, for example, lithium diisopropylamide, lithium 2,2,6,6-tetramethylpiperidide and the like are used. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, andpref erred examples of such solvent include, but are not limited to, ether-based solvents such as diethyl ether, tetrahydrofuran or dioxane, dimethoxyethane, and the like, as well as benzene, toluene and the like. The reaction temperature is from -78°C to room temperature. As the oxidizing reagent for oxidizing the alcohol of compound 2, for example, manganese dioxide, sulfur trioxide-pyridine complex, N-methylmorpholine-N-oxide, various kinds of chromic acid oxidizing reagents and the like can be used, and also Swern oxidation, Moffat oxidation and the like may be applied. As the solvent, any solvent can be used
insofar as they are not concerned with the reaction, and examples of such solvent include hydrocarbon halides such as dichloromethane, chloroform and the like, as well as other ethyl acetate, acetonitrile, dimethyl sulfoxide,
N,N-dimethylformamide and the like. The reaction temperature is usually from -78 °C to reflux temperature of the solvent. The reaction of cyclizing compound 3 with the use of hydrazine monohydrate may be carried out either in the absence of solvent or in the presence of solvent. Any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane, alcohol solvents such as methanol, ethanol or propanol, as well as pyridine, dimethyl sulfoxide, benzene, toluene and so on. The use amount of hydrazine monohydrate is from 2 to 20 equivalents with respect to the material. The reaction temperature is usually from 0°C to reflux temperature of the solvent. Production method 2
(Formula Removed)
Compound 2 can alsobe produced in accordance with Production method 2. Aromatic ring compound 1 is made into a metal aryl in accordance with Production method 1, which is then reacted with formylation agent, to thereby produce compound 4 . Examples
of formylation agent include N,N-dimethylf ormamide, N-formylpiperidine, methylphenylformamide and so on. As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of which include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane, dimethoxyethane and the like, as well as benzene, toluene and the like. The reaction temperature is from -78 °C to room temperature. Compound 2 can be produced by making a metal aryl or metal halogenoaryl to react on compound 4 . The metal aryl or metal halogenoaryl can be readily prepared, for example, by converting halogenoaryl into aryl lithium or metal halogenoaryl by using alkyl lithium, magnesium, zinc and the like. As the alkyl lithium, for example, AHoutyllithium, sec-butyllithium, tert-butyllithium, phenyl lithium and the like can be used, and N,N,N' N-tetramethylethylenediamine, hexamethylphosphoramide and the like additives may be used as necessary. As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane, dimethoxyethane and the like, as well as benzene, toluene and the like. The reaction temperature is from -78°C to room temperature. Production method 3
(Formula Removed)
Compound (I) can be produced by cyclizing compound 4 in accordance with Production method 1 using hydrazine to render indazole compound 5, halogenating 3-position of compound 5 to render it compound 6, protecting 1-position of pyrazole of compound 6 to render it composition 7, and then introducing a substituent into 3-position by coupling reaction, followed by deprotection at 1-position. The R1 which may be introduced by coupling reaction has the same meaning as defined above, and the case where h=j=0 is preferred. Compound 8 wherein an aromatic ring is directly bonded to 3-position of pyrazole can be produced by Suzuki coupling with aryl boronic acid or by Stille reaction with aryl trialkyl tin or the like. Furthermore, in particular, compound 8 having styrene (h=j=0,k=l) at 3-position can also be produced by coupling based on Heck reaction in which styrene is reacted on compound 7, besides the Suzuki coupling. Themethod for producing 3-styrene compound 8 is described in Production methods 35 to 4 0 below. As a ha logenat ion reagent for 3-position, for example, N-bromosuccinimide, N-iodosuccinimide,
N-chlorosuccinimide, bromine, iodine and the like are used, and radical initiators such as 2,2'-azodiisobutyronitrile or benzoyl peroxide or bases such as sodium hydroxide may be added as necessary. The use amount of the halogenation reagent is from 1.05 to 1.2 equivalents with respect to the material. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane, chloroform, carbon tetrachloride and the like, as well as ethyl acetate, acetonitrile, dimethyl sulfoxide, N,N-dimethylformamide and so on. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Examples of the protective group at 1-position include tert-butoxycarbonyl group, p-toluenesulfonyl group, trityl group, methoxymethyl group and the like. Introduction of tert-butoxycarbonyl group and p-toluenesulfonyl group can be achieved by letting compound 6 and di-tert-butyldicarbonate or p-toluenesulf onyl chloride react with each other in the presence of a base. Preferred examples of the base include, but are not limited to, triethylamine, 4-N,N-dimethylaminopyridine and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane, hydrocarbon halides such as dichloromethane or chloroform, as
well as ethyl acetate, acetonitrile, dimethyl sulfoxide, dimethylformamide and so on. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
Introduction of trityl group and methoxymethyl group can be achieved by letting compound 6 and chlorotriphenylmethane or chloromethylmethyl ether react with each other in the presence of a base. Preferred examples of the base include, but are not limited to, sodium hydride, potassium tert-butoxide, lithium diisopropylamide, potassium carbonate, sodium hydroxide and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane, as well as ethyl acetate, acetonitrile, dimethyl sulfoxide, dimethylformamide and so on. The reaction temperature is from -20°C to reflux temperature of the solvent.
Aromatic olefins used for Heck reaction and aryl boronic acids used for Suzuki coupling are commercially available, or may readily be prepared in a conventional manner if not commercially available. Aryl boronic acid can be prepared by converting a halogenoaryl into an aryl lithium or metal halogenoaryl with the use of a alkyl lithium, magnesium, zinc and the like, and letting the aryl lithium or metal halogenoaryl react with a trialkyl borate to render it a boric acid ester, followed by hydrolysis. Examples of the alkyl lithium include N-butyllithium, sec-butyllithium, tert-butyllithium, phenyl
lithium and the like, and additives such as N,N,N' ,N'-tetramethylethylenediamine or
hexamethylphosphoramide may be added as is necessary. The hydrolysis following the reaction between aryl lithium and trialkyl borate to obtain ester borate may be carried out by adding water, or by using an acid such as hydrochloric acid, sulfuric acid. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and preferred examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane . The reaction temperature is from -78 °C to room temperature. Aromatic olefins can be prepared by letting an aryl aldehyde and methylphosphonium ylide react with each other. For example, methyltriphenylphosphonium salt is treated with a base to make it methylphophonium ylide, which is then reacted with an aryl aldehyde in the same system, to thereby produce aromatic olefins. Examples of the base include potassium tert-butoxide, sodium methoxide, sodium hydride, potassium carbonate, sodium hydroxide and the like. Examples of the solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran or dioxane, hydrocarbon halides such as dichloromethane, chloroform, as well as toluene and the like. The reaction temperature is from -20°C to reflux temperature of the solvent.
The use amount of aryl boronic acid used for Suzuki coupling or aromatic olefins used for Heck reaction is from 1 to 3
equivalent(s) with respect to the material. Examples of catalyst to be used include, palladium acetate(II), dichlorobistriphenylphosphine palladium(II), tetrakis(triphenylphosphine) palladium (0) and the like. The use amount of catalyst is about 5% by mole with respect to the material. As is necessary, phosphine ligand, twice in mole of catalyst of a phosphine ligand, for example,
tri-tert-butylphosphine, 2-(di-tert-butylphosphino) biphenyl, 2-(dicyclohexylphosphino) biphenyl, triphenyl phosphine and the like may be added. Examples of the base to be used include sodium hydrogen carbonate, sodium carbonate, potassium carbonate, cesium carbonate, potassium fluoride and the like. As the solvent to be used, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, dimethylformamide, N-methylpyrrolidone, tetrahydrofuran, dioxane, diethylene glycol dimethyl ether, toluene and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Deprotection of a tert-butoxycarbonyl group and a trityl group can be readily achieved by acid. Examples of the acid include hydrochloric acid, sulfuric acid, trifluoroacetic acid and the like. As is necessary, radical scavengers such as thiophenol or tri-iso-propylsilane may be added. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not
limited to, hydrocarbon halides such as dichloromethane or chloroform, alcohol solvents such as methanol or ethanol, as well as anisole and the like. The reaction temperature is -20°C or reflux temperature of the solvent. Deprotection of the tert-butoxycarbonyl group and p-toluenesulfonyl group can be readily achieved by a base. Examples of the base include, but are not limited to, aqueous sodium hydroxide, aqueous potassium hydroxide and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane. The reaction temperature is room temperature or reflux temperature of the solvent. Deprotection of the methoxymethyl group is generally achieved by acid treatment, however, when an aminal which is incompletely-deprotected by acid treatment remains, treatment with ammonia water is carried out to achieve the deprotection. Production method 4
(Formula Removed)
Compound (I) can be produced in the following manner: oxidation of an alcohol which is obtained by metallizing α-position adjoining to the amino group suitably protected by treating compound 9 with a base and nucleophilically adding to an aldehyde, and deprotection of the amino group are successively conducted to thereby obtain aminoketonelO; and then aminoketone 10 is diazotizated and reduced so as to be closed into a pyrazole.
As the protective group for amino group, any groups may be used insofar as they are base-resistant, and preferred examples of such protective group include, but are not limited to, a tert-butoxycarbonyl group, benzyloxycarbonyl group, methoxymethyl group and the like. Conversion of compound 9 into a metal aryl is basically conducted in the manner as described in Production method 1, however, in this case 2 or more equivalents of base is required. Oxidation of alcohol can be achieved in the manner as described in Production method 1. Deprotection of amino group from tert-butoxycarbonyl group, benzyloxycarbonyl group, methoxymethyl group and the like can be readily achieved by acid treatment. The acid to be used is as same as those recited in Production method 3, and as for the benzyloxycarbonyl group and the like, for example, may be deprotected under the condition of catalytic reduction or the like.
In the case where compound 10 cannot be derived directly from compound 9 for such a reason that aromatic aldehyde is relatively difficult to avail, the ortho position of the
protected amino group is formylated to obtain 11, to which a metal aryl, a metal halogenoaryl or the like is nucleophilically added, followedby deprotection of amino group, to thereby obtain compound 10. The condition for formylation is as described in Production method 2.
Conversion of compound 10 into diazonium salt is achieved by letting nitrite esters such as sodium nitrite ester or isoamyl nitrite ester act in the presence of acid. As the reaction solvent, for example, alcohol solvents such as methanol, ethanol, water and the like are used, as the acid, hydrochloric acid, sulfuric acid, acetic acid and the like can be used. The reaction temperature is usually around 0°C.
Reduction of diazonium salt and subsequent ring closing of indazole ring are achievedby letting tin chloride (II), copper chloride (II) or the like reducing agent act in the presence of acid, to thereby obtain hydrazine which is an intermediate, and the use amounts of these are usually from 1 to 10 equivalent (s) with respect the starting material. Generally, compound (I) is obtained as a result of spontaneous ring closing associated with dehydration within the system. As the reaction solvent, for example, alcohol solvents such as methanol or ethanol, as well as hydrochloric acid, sulfuric acid, acetic acid and the like can be used. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
As an alternative method, an amino group is substituted with a suitable halogen atom to make compound 3, which is then
made cyclic in the manner as described in Production method 1 using hydrazine, thereby obtaining compound (I) . Preferred example of the halogen atom is, but are not limited to, a fluorine atom. As a concrete method, Baltz-Schiemann reaction can be recited, andby conducting this reaction, it is possible to derive fluoro ketone 3. Baltz-Schiemann reaction is achieved in the following manner: a nitrite ester such as sodium nitrite ester or isoamyl nitrite ester is caused to act on compound 10 in the presence of tetrafluoroboric acid so as to convert compound 10 into a diazonium tetrafluoroborate salt, followed by thermal decomposition or photodegradation. The nitrite ester or nitrite salt used for diazotization is usually in the amount of from 1 to 2 equivalent (s) with respect to the material, and the reaction temperature is preferably and usually 0°C or less. Besides tetrafluoroboric acid, hexafluorophosphoric acid, hexafluorosilicic acid, hexafluoroantimonic acid and the like may be used. After separating the azonium salt, the thermal decomposition may be achieved by dilution in barium sulfate, barium fluoride and the like, or may be achieved by heating in an organic solvent such as acetone or hexane. In the case where separation of diazonium salt is difficult, the thermal decomposition may be conducted while adding a copper salt such as copper chloride or copper powder in the solution. Production method 5
(Formula Removed)
Alternatively, Compound 13 may be produced in the manner
as described in Production method 5. Herein, R1 are as defined, however, this also achieved in the case where R1 is a methyl group as is a hydrogen atom. The protective group recited herein preferably designates an amino group or carbamoyl group although such protective group may be absent. That is, starting from a precursor, compound 13 wherein an amino group or its equivalent functional group and an active methyl group or an active methylene group are adjacent with each other on the aromatic ring, the amino group or its equivalent functional group is made into nitroso compound using a nitrite salt or nitrite ester, going through the reaction intermediate as described above, and then the intramolecular dehydration condensation with the adjoining active methyl group or methylene group in the presence of a suitable acid or base in the reaction system is carried out, to thereby lead pyrazole ring compound 8. As the reagent used for conversion into nitroso, nitrite salts or nitrite esters can be exemplified. Examples of nitrite esters include, but are not limited to, isoamyl nitrite ester and t-butyl nitrite ester, and examples of nitrite salts include sodium nitrite ester, potassium nitrite ester and the like. In particular, when a nitrite salt is used, a phase transfer catalyst such as crown ether may be used together. The use of the nit rite salt or nitrite
ester is from 1 to 10 equivalent (s) with respect to the material. As the amino group or its equivalent functional group, amino groups such as acetamide are preferred without limitation, and in such a case, acetic anhydride is used as a solvent or present together in most general cases. General examples of the condensing reagent and base include, but are not limited to, sodium acetate and potassium acetate, and the use amount is usually from 1 to 10 equivalent(s). As the reaction solvent, any solvents besides acetic anhydrous can be used insofar as they are not concerned with the reaction, however, by conducting the reaction in, for example, but are not limited to, hydrocarbon solvents such as benzene, toluene or xylene, halogen solvents such as chloroform or 1,2-dichloroethane, dioxane, glacial acetic acid, as well as acidic solvents such as hydrochloric acid or sulfuric acid, it is possible to complete the condensation. The reaction temperature is from 0°C to reflux temperature of the solvent.
In the following, concrete Productionmethod of the compound represented by the general formula (II) including the Production method for side chain moiety, however it is to be noted that the Production method is not limited thereto.
From Production method 6 to Production method 12, Q designates a nitrogen atom, and at least one atom other than Q among atoms existing on the aromatic ring in which Q is involved designates -CH=. In this case, Q is a generic designation for Q1 to Q4 in the above general formula (II) and means either 1,
(Formula Removed)
After treating Halogenoketone compound 14 obtained by using
Production method 1 and so on with a peroxide to lead it into iV-oxide 15, a various kinds of reagents are caused to act on N-oxide 15, to thereby obtain compound 16 wherein a substituent is introduced onto a carbon atom around which no substitution occurs and hence generally having predominant orientation. As the functional group which can be introduced by using N-oxide as a starting material, a cyano group, halogen group, acyloxy group, alkoxy group and the likecanbe exemplified. Theobtained compound 16 is made cyclic in accordance with Production method 1 using hydrazine, to thereby produce compound (II)-a.
In the method for leading compound 14 into N-oxide, for example, peroxides such as m-chloroperbenzoic acid, benzoyl peroxide or hydrogen peroxide are used, and the use amount of peroxide is from 1 to 10 equivalent (s) with respect to the material. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane, chloroformor1,2-dichloroethane, hydrocarbon solvents such as benzene and toluene, as well as water, acetic acid and the like. The reaction temperature is usually from
0°C to reflux temperature of the solvent.
As shown in J. Org. Chem. 1983, 48, 1375, for example, the method of cyanizing the adjoining carbon atom by using N-oxide is achieved by causing the cyanizing reagent directly to act or causing the cyanizing reagent to act in the presence of acid chloride. Examples of reagent for direct cyanation include sodium prussiate, potassium prussiate, trimethylsilyl cyanide, diethylcyano phosphate and the like, and bases such as triethylamine or 1,8-diazabicyclo[5.4.0]unde-7-cene may be present together in the reaction system. It is also possible to practice the above reaction with the cyanizing reagent in the presence of acid chloride, and examples of such acid chloride include benzoyl chloride, N,N-dimethyl, carbamoyl chloride and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of which include, but are not limited to, halogen solvents such as dichloromethane, chloroform, 1,2-dichloroethane and the like, and polar solvents such as acetonitrile, N,N-dimethylf ormamide or dimethyl sulfoxide, dimethylsulfuric acid and the like. The use amounts of cyanizing reagent, base and acid chloride are from 1 to 10 equivalent (s) with respect to the material. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Examples of the reagent for halogenating the adjoining carbon atom by using N-oxide include, phosphorus oxychloride, phosphorus oxybromide, benzoyl chloride, p-toluene sulfonyl
chloride, ethyl chloroformate, trifluoromethanesulfonyl chloride, thionyl chloride and the like. The reaction may be carried out in the presence or absence of solvent, and as such a solvent any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane, chloroform or 1, 2-dichloroethane, hydrocarbon solvents such as benzene or toluene, ether solvents such as tetrahydrofuran, N,N-dimethylformamide and the like. The use amount of the solvent is from an equivalent with respect to the material to solvent amount. When the substrate is unstable to acid, a base such as triethylamine may coexist in the reaction system. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
As the reagent for acyloxylation of the adjoining carbon atom of N-oxide, carboxylic anhydrides are usually used. The use amount is from an equivalent with respect to the material to solvent amount. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Alkoxylation of the adjoining carbon atom of N-oxide can be achieved by causing ethyl chloroformate, p-toluenesulfonyl chloride or the like to act in the presence of a base in alcohol. The use amount is from 1 to 10 equivalent(s) with respect to the material. As the base, metal alkoxides, triethylamine and the like are used, and the use amount is from 1 to 2 equivalent (s) with respect to the material. As the solvent, any solvents can
be used without particular limitation insofar as they are not concerned with the reaction, however, it is general to conduct the reaction in a solvent of alcohol which corresponds to the alkoxy group intended to be introduced. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Production method 7
(Formula Removed)
Introduction of functional group using the similar manner as described in Production method 6 is also applicable to the nitrogen-containing condensed pyrazole compound 17. That is, after oxidizing a desired nitrogen atom Q and leading into N-oxide by protecting the pyrazole ring of compound 17 and letting a peroxide act on compound 18, various kinds of reagents are made to act on the N-oxide so as to introduce a substituent into an adjoining carbon atom having predominant orientation. Then deprotection is conducted to produce Compound (II)-a.
Protection and Deprotection of a nitrogen-containing condensed pyrazole ring are achieved in the manner as described in Production method 3. The process of protection and deprotection of pyrazole ring may be eliminated from Production method, however, it is preferred to employ the process in consideration of yield and versatility.
Production method 8
(Formula Removed)
In the reactionbetween N-oxide and various kinds of reagents as described in Production method 6, when it is difficult to directly introduce a functional group other than halogens into a carbon atom at a desired position because of orientation of the reaction regent, the functional group may be indirectly introduced to the desired position as is described in Production method 8 . That is, as an alternative method of Production method 6, first compound 19 wherein a carbon atom at a position of predominant orientation is substituted with a halogen is obtained by halogenating N-oxide, then compound 20 wherein a functional group is introduced to a desired position in the similar manner as described in Production method 6 is obtained, and then compound 20 is dehalogenated by reduction, to afford objective intermediate 16. Compound 16 is then made cyclic with hydrazine in accordance with Production method 1, to there by obtain Compound (II) . As the halogen T3, chlorine, bromine, iodine which are easy to leave can be exemplified, with chlorine and bromine being preferred for which commercially available phosphorous oxychloride, phosphorous oxybromide and the like can be used.
As the method for reducing the halogen group introduced
into compound 20 to lead compound 16, for example, hydrogenation using palladium-carbon, palladium hydride-carbon, platinum oxide, Raney nickel and the like catalyst, or the condition of zinc-acetic acid, copper-acetic acid and the like are used. As other conditions, the condition of hydrazine, palladium-carbon and the like are also known. As the solvent for hydrogenation, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol, ethanol, halogen solvents such as dichloromethane and chloroform, ether solvents such as tetrahydrofuran or diethyl ether, as well as ethyl acetate, dimethylformamide, toluene and the like. The use amount of catalyst for hydrogenation is from 5% to 20% by weight with respect to the material. The pressure of hydrogen is usually atmospheric pressure to 5 atm. The reaction temperature is usually from room temperature to reflux temperature of the solvent. In the catalytic reduction, more gentle reaction can be achieved in the presence of a base, such as for example, sodium hydroxide, potassium hydroxide, triethylamine and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent. As the solvent for Zinc-acetic acid, copper-acetic acid and the like, glacial acetic acid or hydrous acetic acid and the like are used, and the use amount of metal is from 3 to 10 equivalents with respect to the material. The reaction temperature is usually reflux temperature of the solvent.
Production method 9
(Formula Removed)
In the similar manner as described in Production method 8, when it is difficult to directly introduce a functional group other than halogens into a carbon atom at a desired position because of orientation of the reaction regent acting on N-oxide, the similar way as described in Production method 8 can be generally applied to nitrogen-containing condensed pyrazole compounds. That is, after oxidizing compound 17 with a peroxide while protecting 1-position of pyrazole of compound 17 to lead N-oxide, the N-oxide is halogenated, thereby producing compound 21 of predominant orientation. Next, compound 21 is treated again with a peroxide and allowed to react with a suitable reagent so as to introduce a substituent to a carbon atom at a more desirable position, followed by dehalogenat ion and deprotection, to thereby produce Compound (II)-a. Protection and deprotection of compound 17 can be achieved in the manner as described in Production method 3. Production method 10
(Formula Removed)
As described in Production method 8 and the like, in the

case where the substitution position of the halogen atom which is introduced by causing a halogenation reagent such as phosphorus oxychloride to react on N-oxide is ortho- or para-position of the nitrogen atom constituting the ring, by letting amines to act, it is possible to readily substitute the halogen atom to obtain aniline 22. Aniline 22 can be converted into either primary, secondary or tertiary amine without limitation, and preferably to a primary amine. For introducing a primary amine, a variety of methods are applicable, for example, a method of causing imino chloride to act on the hydrazine and conducting catalytic reduction to lead an amine; a method of introducing an amine by using liquid ammonia or concentrated aqueous ammonia; or a method of causing potassium phthalimide to act and then introducing amine by hydrolysis using hydrazine or the like. Using the above reaction, it is possible to convert halide 21 into aniline 22.
The reaction with hydrazine is conducted, for example in alcohol solvents such as methanol or ethanol, and various kinds of solvents suchas toluene, benzene, tetrahydrofuranordioxane . The use amount of hydrazine is usually from 1 to solvent amount. The reaction temperature is usually from room temperature to reflux temperature of the solvent. In the method for catalytic reaction, Raney nickel is generally and often used. As the reaction solvent, hydrous alcohol solvents and the like are exemplifies. Amination using ammonia can be readily achieved by causing a large excess of ammonia to act. The reaction may
be carried out after diluting with alcohol solvents such as
methanol or with water, or liquid ammonia may be directly caused
to act. The reaction may be carried out in a sealed tube for
some cases. The reaction temperature is usually from -78°C to
reflux temperature of the solvent. The use amount of potassium
phthalimide is usually from 1 to 2 equivalent (s), and as the
reaction solvent, any solvents insofar as they are not concerned
with the reaction can be used, and preferred examples of such
solvent include, but are not limited to, N, N-dimethylformamide,
acetonitrile, dimethyl sulfoxide, pyridine, tetrahydrofuran,
dioxane or the like, as well as alcohols such as methanol or
ethanol. The reaction system may contain a base, and examples
of such base include triethylamine, diisopropylethylamine,
pyridine and the like. The reaction temperature is usually from
room temperature to reflux temperature of the solvent.
Deprotection of phthaloyl group thus introduced can be practiced
generally in strong base or reduction condition, and examples
of which include hydrazine, sodium sulfide, sodium boron hydride
and the like. Preferably, the deprotection is achieved by
causing hydrazine to act in ethanol at room temperature. The
use amount is from 1 equivalent to solvent amount with respect
to the material.
Production method 11
(Formula Removed)
N-oxide 23 produced in Production method 7 or the like can be led to compound 24 by letting an acid anhydride act on W-oxide as is exemplified in Production method 6, followed by acyloxylation. In the case where the substitution position of the introduced acyloxy group is ortho- or para- position of the nitrogen atom constituting the ring, it is readily hydrolyzed to be led to pyridone compound (II)-b. As the hydrolyzing reagent, hydrochloric acid, sulfuric acid, p-toluenesulfonic acid, as well as alkalis such as sodium hydroxide, potassium hydroxide or potassium carbonate are exemplified, and the hydrolysis can be readily achieved in aqueous solutions, hydrous alcohol solvents and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Production method 12
(Formula Removed)
In the case where the substitution position of the hydroxyl
group introduced by Production method 11 or the like is ortho-or para- position of the nitrogen atom constituting the ring, by letting a variety of halides on compound 25 in the presence of a base, it is possible to obtain compound 26 wherein a
substituent is introduced to the nitrogen atom. As the base tobeused, sodiumhydride, potassium carbonate, cesiumcarbonate and the like are exemplified, and the use amount is usually from 1 to 2 equivalent (s) . Examples of the halide to be used include, but are not limited to, bromides and iodides, and the use amount thereof is usually from 1 to 3 equivalent (s) with respect to the material. The halides which may be aliphatic or aromatic halides optionally have a suitable functional group. In the case of aromatic halides, iodides are especially preferred, and by adding a metal catalyst such as copper iodide in the presence of a base, it is possible to achieve excellent result. The use amount of metal catalyst is usually from a catalyst amount to 1 equivalent. In the cases of aralkyl halides, allyl halides and the like which are highly reactive, a salt such as sodium iodide is used together instead of base. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane, chloroform or 1,2-dichloroethane, hydrocarbon solvents such as benzene or toluene, ether solvents such as tetrahydrofuran, and polar solvents such as N,N-dimethylformamide or acetonitrile. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
By reacting a silylation reagent such as trimethylsilyl chloride with compound 25 in the presence of a base, it is possible to obtain compound 27 in which a hydroxyl group is silylated.
Examples of the base to be used include, but are not limited to, A/-butyllithium, sec-butyllithium, tert-butyllithium, phenyl lithium, triethylamine, potassium carbonate and the like, and the use amount is from 1 to 2 equivalent (s) . Examples of silylation reagent include, trimethylsilyl chloride and trimethylsilyl trifluoroacetate, trimethylsilyl trifluoromethanesulfonate and the like, and the use amount is from 1 to 2 equivalent (s) . As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane, chloroform or 1,2-dichloroethane, hydrocarbon solvents such as benzene or toluene, and ether solvents such as tetrahydrofuran. The reaction temperature is from -78°C to reflux temperature of the solvent.
It is possible to enable compound 27 to act as a nucleophile by introduction of trimethylsilyl group or the like. As a result of this, compound 27 nucleophilically reacts with a variety of reagents V in the presence of a Lewis acid, to forma corresponding adduct 26 . Examples of the reagent that is reactive with compound 27 having such a property include, but are not limited to, Michael receptors such as epoxide, aldehyde, ketone or conjugate enone, and the use amount is from 1 to 2 equivalent (s) with respect to the material. Examples of the Lewis acid include, but are not limited to, aluminum chloride, boron trifluoride -diethyl ether complex and the like, and the use amount is from 1 to 2
equivalent (s) . As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane, chloroform or 1,2-dichloroethane, hydrocarbon solvents such as benzene or toluene, and ether solvents such as tetrahydrofuran. The reaction temperature is from -78°C to reflux temperature of the solvent.
Deprotection of the protective group in compound 26 is carried out in the similar manner as described in Production method 3, whereby corresponding (II)-C is obtained.
The compounds embraced in the general formula (I) or (II) include a group of compounds which are readily produced by a production method other than the production method using the above-exemplified production process, which is specific to the forming condensed pyrazole ring. Now, production methods which are useful for production of specific heterocycle are exemplified in Productionmethod 13 to Productionmethod 19 . As for synthesis of this group of compounds, it goes without saying that these compounds may be produced by using the above-described production method and the production method is not limited to the following production methods.
Production method 13
(Formula Removed)
In Q1 to Q4 in general formula (II), 1H-pyrazolo [3,4-b]
pyridines wherein only Q4 is -N= can be synthesized in the manner as described, for example, in Production method 13 . In general, by letting malonaldehyde or its equivalent act on 5-amino-2ff-pyrazoles 28 in accordance with the known method (Synthesis . 1987,1124) , it is possible to form 2ff-pyrazolo [3,4-b] pyridine ring 29. In this condensing reaction, when malonaldehyde or its equivalent has a suitable functional group, direct introduction of the substituent into the 5-position of the nitrogen-containing pyrazole ring by condensation is possible. As the equivalent of malonaldehyde having a suitable functional group, but are not limited to, sodium 2-cyano-3, 3-dimethoxy-l-propenolate is preferably exemplified, and using this, it is possible to synthesize IH-pyrazolo [3,4-b] pyridine ring 29 wherein a nitrile group is introduced into 5-position. Similarly by using sodium 2-nitromalonaldehyde, it is possible to produce 1H-pyrazolo [3,4-b] pyridine ring 29 wherein a nitrile group is introduced into 5-position. As the solvent for condensation reaction, any solvents are used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, water and the like. The reaction may be carried out in the presence of an acid for neutralizing alkaline or in an acidic solvent, and as the reaction solvent, acetic acid, hydrochloric acid, sulfuric acid and the like can be exemplified. The use amount of malonaldehyde or its equivalent is usually from 1 to 3 equivalent (s) with respect to the material, and the
reaction temperature is usually from room temperature to reflux temperature of the solvent.
By deprotecting compound 29, it is possible to produce compound 30. As the protective group, any groups can be used insofar as they do not influence on the reaction, and examples of such group include, but are not limited to, a benzyl group, benzyloxycarbonyl group, methoxymethyl group, tert-butoxycarbonyl group, trityl group and the like.
Deprotection of benzyloxycarbonyl group, methoxymethyl group, tert-butoxycarbonyl group and trityl group can be achieved in accordance with Production method 3.
Deprotection of benzyl group can be achieved, for example, by the method of letting a Lewis acid act, the method of acidically hydrolyzing, as well as the method of using active hydrogen such as the case of catalytic reduction. As a special example, it is known that heating in pyridine hydrochloride achieves deprotection. As the catalyst for catalytic reduction, palladium-carbon, platinum oxide and the like are used as is described in Production method 8, but other cases use sodium hydride or the like as active hydrogen species. As a Lewis acid, aluminum chloride is preferably used in the amount of from 1 to 10 equivalent (s) . As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane or chloroform, benzene and the like. The reaction temperature is from room temperature

to reflux temperature of the solvent. As the reagent used for the purpose of acidic deprotection, various chromic acids, permanganic acid, cerium ammonium sulfate, selenium dioxide and the like are exemplified, and various chromic acids and permanganic acid are generally causedtoactinanacidic solvent. As the reaction solvent, for example, sulfuric acid, acetic acid, hydrochloric acid, water, acetonitrile, acetone or the like is usedsingly. Alternatively, by using a phase transfer catalyst, the reaction is conducted in a double phase reaction system made up of an organic solvent such as dichloromethane or an acidic solution containing an oxidizing reagent. The oxidizing reagent is used in an amount of 1 to 5 equivalent (s) with respect to the material, and the reaction temperature is usually from room temperature to reflux temperature of the solvent. Production method 14
(Formula Removed)
In Q1 to Q4 in the general formula (II), 1H-pyrazolo [3,4-b] pyrazines wherein Q1 and Q4 are -N= can be synthesized in the manner as described in Production method 14. After leading 5-amino-lH-pyrazoles 28 representedby Production method 13 into
nitroso compound 31, a carbonyl compound whose a position is substituted is condensed in accordance with the known method (J.Chem. Thechnol. Biotechnol. 1990,49(4) , 311-320 or the like) , to thereby form lH-pyrazolo [3,4-b] pyrazine ring 32 . Preferred examples of the reagent having a suitable substituent include, but are not limited to, cyanoacetic acid or its ester, cyanoacetoaldehyde, malonic halide half ester and the like.
By intermolecular dehydration condensation between compound 31 and the above reaction reagent, amides or imines having an active methylene can be obtained. By treating these amides or imines having an active methylene with a suitable base, the formed anion is dehydration-condensed with the nitroso group in the molecule, with the result that compound 32 is obtained. Depending on the reagent to be condensed and reaction condition, a hydroxyl compound as is compound 33 is sometimes separated, however, by halogenation and reduction of the hydroxyl group so as to remove the hydroxyl group, it can be led into compound 32.
Generally, nitroso reaction is achieved by letting a nitrite ester or a nitrite salt act on a substrate in an acidic solvent. As the reaction solvent, a strong acid such as diluted hydrochloric acid, as well as a mixture thereof with an alcohol such as ethanol can be used. Examples of the nitrite salt or nitrite ester to be used include sodium nitrite ester, sodium nitrite ester, isoamyl nitrite ester and the like, and the use amount is from 1 to 3 equivalent (s) with respect to the material.

The reaction temperature is usually around 0°C.
As the solvent for intermolecular condensation reaction, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, ether solvents such as 1,2-dimethoxyethane or tetrahydrofuran, N,N-dimethylformamide and the like. The solvent may contain a base for neutralizing the acid occurring in the system as is necessary, or by containing an excess base, intramolecular condensation can be achieved concurrently in one pot.
As the solvent for intramolecular condensation reaction, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, ether solvents such as 1,2-dimethoxyethane or tetrahydrofuran, N,N-dimethylformamide, water and the like, containing a base. As the base to be used, metal alkoxide, sodium acetate, sodium hydride, tert-butoxy potassium and the like are exemplified, and the use amount is from 1 to 3 equivalent(s) with respect to the material. As other reaction solvents, the reaction may be conducted in a basic solvent of either one of pyridine, triethylamine, picoline and the like.
The use amount of reagent used for condensation of cyanoacetic acid ester, cyanoacetoaldehyde and the like is usually from 1 to 3 equivalent (s) , and the reaction temperature is usually from room temperature to reflux temperature of the
solvent.
As the halogenation reagent for compound 33, phosphorus oxychloride, phosphorusoxybromide, phosphorouspentachloride, dichlorophenylphosphine and the like can be exemplified. The use amount is usually from 1 to 5 equivalent (s) with respect to the material, and as the reaction solvent, hydrocarbon solvents such as benzene or toluene, N,N-dimethylformamide and the like are used, however, phosphorus oxychloride, for example may be used in absence of solvent. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Next, in accordance with Production method 9 or the like, the halogen group is reduced and deprotected, to obtain compound 32.
Production method 15
(Formula Removed)
IH-pyrazolo [3,4-b] pyrazine ring compound 32 can also be
produced in accordance with Production method 15 as well as Production method 14 . That is, considering that after reducing nitroso compound 31 described in Production method 14 into diamine 34, a substituent is selectively introduced to 5-position of a pyrazolopyrazine ring, in accordance with the known method (Farmaco. Ed. Sci. 1982, 37, 116 or the like) , preferably a glyoxal equivalent is made to act, to thereby obtain compound 35 . Introduction of substituent into compound 35 can be achieved
in the manner as described in Production method 7 . As the method of reducing a nitroso group, for example, hydrogenation using palladium-carbon, palladium hydroxide-carbon, platinum oxide, Raney nickel and the like as a catalyst, or the condition of zinc-acetic acid, copper-acetic acid or the like is used. Also other conditions such as hydrazine or palladium carbon are known . As the solvent for hydrogenation, any solvents are used insofar as they do not inhibit the reaction, and the examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, halogen solvents such as dichloromethane or chloroform, ether solvents such as tetrahydrofuran or diethyl ether, as well as ethyl acetate, dimethylformamide, toluene and the like. The use amount of the catalyst for hydrogenation is 5% to 20% by weight with respect to the material. The pressure of hydrogen is usually from atmospheric pressure to 5 atm. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Production method 16
(Formula Removed)
lif-pyrazolo [3,4-b] pyrazine ring compound 32 may be
produced in accordance with Production method 16 as is the known method (J. Org. Chem. 1993, _58(22) ,6155-6157) . After treating compound 31 with chloroformic acid ester or the like to render it a cyclic compound 36, various kinds of enamine is caused to
act, to thereby obtain pyrazolopyrazine ring 37 wherein a substituent is selectively introduced to 5-position. Then compound 37 is deprotected in accordance with Production method 3 or the like, thereby obtaining compound 32. Production method 17
(Formula Removed)
1/i-pyrazolo [4,3-d] pyrimidine ring 41 can be synthesized by a variety of ring-closing reactions, and preferably it can be synthesized according to Production method 17. That is, starting from 4-amino-1H-pyrazole-2-yl carboxylic acid ester 38 obtainedby the knownmethod (Farmaco, Ed. Sci 1984, 39 (7) , 618) , formamide is caused to act in the known manner (Chem. Pharm, Bull. 1983, yi, 1228) to obtain 2H-pyrazolo [4,3-d] pyrimidine-7-ol 39, and then the alcohol is removed and a substituent is introduced to 5-position in the manner as described in the above Production method 14 . That is, following halogenation of compound 39 in accordance with Production method 14, pyrazole is protected in accordance with Production method 3 to produce compound 40. Then a substituent is introduced to 5-position in the method as described in Production method 7 or the like, followed by dehalogenation by reduction and
deprotection, to thereby produce the objective compound 41. As the solvent for the condensation reaction with formamide, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as ethanol, ether solvents such as tetrahydrofuran or diethyl ether, and hydrocarbon solvents such as benzene or toluene. The use amount of formamide is from 1 equivalent with respect to the material to solvent amount, and the reaction temperature is from room temperature to reflux temperature of the solvent.
Production method 18
(Formula Removed)
As other construction methods for 1H-pyrazolo [4,3-d] pyrimidine ring compound 41, various kinds of pyrazole analogs can be selected for a starting material as described in Production method 18. As a compound which is analogous to
4-amino-lH-pyrazole-2-yl carboxylic acid ester 38 shown in Production method 17, compound 42 synthesized by the known method (Bioorg. Med. Chem, Lett. 2000, 1/7 (10) , 1983-1986) and the like can be preferably exemplified.
Compound 42 is a useful intermediate for constructing a ring by various kinds of cyclization reactions as is shown in Production method 18. For example, by condensing compound 42 by action of formic acid or alkyl orthoformate by way or a known method (Phrmazie, 1996, 51 (12) , 983-984) , it is possible to obtain lH-pyrazolo [4,3-d] pyrimidine-7-ol derivative 43 or compound 39. Furthermore, from compound 39, by successively conducting halogenation, protection, peroxidation, actions of various reagents to N-oxide, reduction and deprotection in accordance with the above-mentioned Production method 17, it is possible to produce compound 41 wherein a functional group is introduced to 5-position.
In accordance with the known method (J. Med. Chem. 1988, 31, 454), compound 44 which is easily obtained from compound 42 by dehydration may be condensed with a suitable carboxylic acid anhydride so as to introduce a functional group to 5-position.
Similarly, compound 42 may be condensed with various kinds of carboxylic acid derivatives, to produce cyclic compound 43. As a condensing reagent to be acted on 42, acid chlorides and acid anhydrides are preferably used. Depending on the reagent to be used, cyclization proceeds stepwise through intermediate

45. With regard to condensation ring-forming reaction as
described above, reference is made to the known method
(Heterocycles. 2000, 53(12), 2643-2652) for detail. It can be
obtained by halogenating and reducing 1H-pyrazolo [4,3-d]
pyrimidine-7-ol 43 in which 5-position is substituted by
condensation in accordance with Production method 14.
Production method 19 —
(Formula Removed)

1H-pyrazolo [4,3-e] [1,2,4] triazine ring compound 50 can be readily constructed (synthesized) by using, for example, serine derivative 46 as a starting material. In accordance with a regular method, an amino group of compound 4 6 is turned to dimethylaminomethylidene, a formyl equivalent such as compound 47 is introduced, and then compound 47 is treated with hydrazine in accordance with the known method (J. Het. Chem. 1985, 22 (2) , 409), whereby 6-hydroxytriazine ring is constructed. Subseguently, the resultant compound is oxidized by using the method of Production method 1 or the like, to produce compound 48, which is again treated with hydrazine in accordance with
the known method (Pharmazie, 1984, 39(1), 504), thereby constructing 1H-pyrazolo [4,3-e] [1,2,4] triazine ring compound 49. Subsequently, a side chainmoietyisintroduced in themanner as described in Production method 7 or the like, to produce compound 50.
The followings are concrete production examples for the compounds represented by the general formula (I)-A including production methods for the side chain moiety, however, it is to be understood that production methods are not limited thereto. The general formula (I)-A represents a compound in which Cy is a 5-membered heteroaryl among the compounds represented by general formula (I) . In this formula, U1 to U3 each independently designate -O-,-NV1-, -SV-,-CV=,-N= or -CO-, and at least one of U1 to U3 designates -O-, -NV1-, -SV- or -N= and U designates -OH, -NH2 or -SH.
Production methods 20 to 22 describe for the case where U1 at 4-position is a hetero atom.
Production method 20
(Formula Removed)
In the case where U1 is a hetero atom and at least one of
U2 and U3 is -CH= in the general formula (I)-A, by introducing a variety of functional groups into a position corresponding to U2 or U3 of compound 52 obtainable by protecting 1-position of compound 51 by way of electrophilic substitution reaction
using the orientation, followed by deprotection, it is possible to produce Compound (I)-A-l. As the functional group which may be introduced by way of electrophilic substitution reaction, halogen groups, sulfon groups, nitro group, acyl groups and the like can be exemplified.
Halogenation can be achieved in accordance with Production method 3.
As a sulf onation method, a reaction in the presence of proton such as sulfuric acid, a condition using pyridine sulfur trioxide where there are no protons and the like are used. The use amount of sulfonation reagent is from 1.05 to 1.2 equivalent (s) with respect to the material. The reaction may be conducted in the absence of solvent or in the presence of solvent. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane, chloroform or carbon tetrachloride. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
As a nitration method, concentrated nitric acid, fuming nitric acid, mixed acid of nitric acid and sulfuric acid, mixture of sodium nitrate or potassium nitrate and sulfuric acid, acetyl nitrate, trifluoroacetyl nitrate, as well as nitronium salts such as nitronium trifluoromethanesulfonate or nitronium tetrafluoroborate and the like are used. The use amount of nitration reagent is from 1.05 equivalents with respect to the
material to solvent amount. The reaction may be conducted in the absence of solvent or in the presence of solvent. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane, chloroform or carbon tetrachloride, hydrocarbons such as hexane or pentane, and when a nitronium salt is used, sulfolane or acetonitrile can be used. The reaction temperature is usually from -20°C to reflux temperature of the solvent.
As an acylation method, Friedel-Crafts reaction is preferably used.
In the case where U1 is a NH group, the group may be protected with a suitable protective group in the similar manner as described for protection of 1-position, and then deprotected in the final step. Protection and deprotection of 1-poition is achieved in the manner as described in Production method 3.
Production method 21
(Formula Removed)

Compound (I)-A-2 in which a substituent is introduced to the position corresponding to U2 can be produced by protecting 1-position of pyrazole of compound 53 wherein U2 is -CH= to obtain compound 54, and converting compound 54 into a metal aryl with the use of alkyl lithium, lithium amide or the like, introducing
a substituent V in accordance with the Production method 6, and then conducting deprotection. As the functional group which may be introduced, halogen groups, formyl group, acyl groups, azido group, amino group and the like are exemplified.
Compound (I)-A-2 is produced in the following manner. After converting compound 54 into a metal aryl in accordance with Production method 1, the resultant metal aryl is reacted with a variety of reagents, followed by deprotection. As the variety of reagents, the following reagents can be exemplified. Examples of halogenation reagent include iodine, N-iodosuccinimide, bromine, A7-bromosuccinimide and the like. Examples of formylation reagent include N,N-dimethylformamide, N-formylpiperidine, methylphenylformamide and the like. Examples of azidation reagent include
azidomethyltrimethylsilane, toluenesulfonyl azide and the like.
Protection and deprotection of 1-position of pyrazole ring are achieved in the manner as described in Production method 3.
Production method 22
(Formula Removed)
In the Production method 20, when U2 and U3 are -CH=, and hence it is difficult to selectively introduce a substituent by way of electrophilic substitution reaction using orientation
to U3, first 1-position of pyrazole of compound 55 is protected in the similar manner as described in Production method 21, which is then converted into a metal aryl with the use of alkyl lithium, lithium amide or the like, and then halogenated by action of a halogenation reagent to obtain compound 56 wherein U2 is halogenated; and then a substituent is introduced to U3 by electrophilic substitution reaction, followed by a sequence of reduction and deprotection, whereby Compound (I)~A-3 is obtained.
Protection and deprotection of 1-position are achieved in the manner as described in Production method 3.
Production methods 23 to 24 describe the case where U2 at 5-position is a hetero atom.
Production method 23
(Formula Removed)
In the case where U2 is a hetero atom, and at least one
of U1 and U3 is -CH=, in accordance with the method described in Production method 20, it is possible to produce Compound (I)-A-4 having a substituent at U1 or U3 from compound 58 which is obtained by protecting 1-position of compound 57.
Production method 24
(Formula Removed)
Compound (I)-A-4 having a substituent at U1 or U3 can also be produced in the manner as described in Production method 21.
Production methods 25 to 27 describe the case where U3 at 6-position is a hetero atom.
Production method 25
(Formula Removed)
In the case where U3 is a hetero atom and at least one of
U1 and U2 is -CH=, it is possible to produce Compound (I)-A-5 into which a substituent is introduced at U1 or U2 in the manner as described in Production method 20.
Production method 26
(Formula Removed)
Compound (I)-A-6 in which a substituent is introduced to
the position corresponding to U2 can be produced in the manner
as described in Production method 21.
Production method 27
(Formula Removed)
In the case where U1 and U2 are -CH= and hence it is difficult to selectively introduce a substituent by way of electrophilic
substitution reaction using orientation to U1 in Production method 24 , Compound (I) -A-7 can be produced in the similar manner as described in Production method 22 while protecting 1-position of pyrazole of compound 63 in the similar manner as Production method 25. Production method 28
(Formula Removed)
Methods which are similar to Production methods 20 to 27 may also be generally applied before ring-closing of pyrazole ring. That is, after introducing a substituentV to into compound 65, the pyrazole ring is closed with hydrazine monohydrate, to thereby produce Compound (I)-A. Cyclization reaction of hydrazine monohydrate can be achieved in accordance with Production method 1. Production method 29
(Formula Removed)
In the case where it is difficult to practice Production method 28 in accordance with Production method 1, first compound 67 is separated, which is then cyclized by Ullmann reaction using a copper reagent or coupling reaction using a palladium catalyst, to thereby produce Compound (I)-A.
Hydrazine may be reacted with compound 66 without being
protected, however, hydrazide which is protectd with an acetyl group or the like is preferably used. This reaction may be conducted in the absence of solvent or in the presence of solvent. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane, alcohol solvents such as methanol, ethanol or propanol, as well as pyridine, dimethyl sulfoxide, benzene, toluene and the like. The use amount of hydrazine or hydrazide isfrom 2 to20 equivalents with respect to the material. The reaction temperature is usually from 0CC to reflux temperature of the solvent.
As the copper reagent to be usedin Ullmann reaction, copper, copper chloride, copper bromide, copper iodide, copper oxide and the like can be exemplified. The use amount is from a catalyst amount to 2 equivalents with respect to the material. Examples of the base to be used include, but are not limited to, potassium carbonate, sodium carbonate, potassium acetate, sodium acetate and the like. The reaction may be conducted in the absence of solvent or in the presence of solvent. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, toluene, nitrobenzene, diphenyl ether, dimethylformamide, dioxane and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Examples of the palladium catalyst to be used when the reaction is achieved by coupling reaction include, but are not limited to, tris(dibenzylideneacetone)dipalladium, palladium acetate and the like. As a ligand, 2,2'-bis(diphenylphosphino)-1,1'-naphtyl, 1,1'-bis(diphenylphosphino) ferrocene, tri(tert-butyl) phosphine or the like is used in the amount of 1 to 3 equivalent (s) to the catalyst. As the base, sodium tert-butoxide, potassium tert-butoxide, cesium carbonate and the like are preferred. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and preferred examples of such solvent include, but are not limited to, dimethylformamide, tetrahydrofuran, dioxane, diethyleneglycol dimethyl ether, toluene, xylene and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Protection and deprotection of 1-position of pyrazole are achieved in the manner as described in Production method 3. Production method 30
(Formula Removed)
Synthesis of Compound (I) -A is also achieved by the method
in accordance with Production method 5 . That is, using compound 69 wherein an amino group or its equivalent functional group
and an active methyl group or active methylene group are adjacent with each other on the aromatic ring, as a precursor, the amino group or its equivalent functional group is converted to a nitroso group using nitrite salt or nitrite ester. This reaction intermediate is then subjected to intramolecular dehydrating condensation with the adjacent active methyl group or methylene group in the presence of a suitable acid or base, to thereby construct a pyrazole ring. Finally, deprotection is conducted, to produce Compound (I)-A.
Protection and deprotection of 1-position of pyrazole are achieved in the manner as described in Production method 3.
The compounds embraced in the general formula (I) -A include other compounds which are readily produced by a production method other than the production method using the above-exemplified production process, which is specific to the forming condensed pyrazole ring. Now, production methods which are useful for production of specif ic heterocycle are exemplified in Production method 31 to Production method 34. As for synthesis of these group of compounds, it goes without saying that these compounds may be produced by using the above-described production method and the production method is not limited to the following production methods. Production method 31
(Formula Removed)
Compound 69 can be produced in the following manner: compound 69 which is easily in accordance with the known methods (U=hydroxyl group: for example, J. Org. Chem. 1992, 57, 5680-5686., U=thiol group: for example, J. Heterocycle. Chem. 1990, 21_, 567., U=amino group: for example, Synthesis 1987, 1124.) is iodized or brominated at 4-position of pyrazole to obtain compound 70; trimethylsilyl acetylene is coupled to this compound 70 by Sonogashira coupling and detrimethylsilylation is conducted to obtain compound 71; and then compound 71 is aromatized and deprotected.
By halogenating 4-position of compound 69 in accordance with Production method 1, it is possible to produce compound 70. As the halogen atom to be substituted, iodine or bromine is preferred.
Trimethylsilyl acetylene used for Sonogashira coupling is commercially available one. The use amount of trimethylsilyl acetylene is from l to 3 equivalent (s) with respect to the material. Examples of catalyst to be used include, but are not limited to, palladium acetate (II), dichlorobistriphenyl phosphine palladium (II), tetrakis(triphenylphosphine)palladium (0) and
the like. The use amount of catalyst is about 0.1% by mole with respect to the material. As is necessary, it is possible to add an equivalent or twice the catalyst amount of an additive, for example, copper iodide (I), triphenylphosphine and the like. Examples of the base to be used include, but are not limited to, triethylamine, diisopropylamine, piperidine and the like. As the solvent, any solvents can be used insofar as they do not inhibit the reaction, and preferred examples of such solvent include, but are not limited to, dimethylformamide, tetrahydrofuran, dioxane, diethyleneglycol dimethyl ether, toluene, xylene and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Detrimethylsilylation can be readily achieved using fluorine anion or acid. As the fluorine anion, tetrabutylammonium fluoride, hydrogen fluoride, potassium fluoride, cesium fluoride and the like can be used. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane or chloroform, alcohol solvents such as methanol or ethanol, as well as water, diethyl ether, tetrahydrofuran, dioxane, toluene and the like. The reaction temperature is from -20°C to reflux temperature of the solvent. As the acid, hydrochloric acid, sulfuric acid, trifluoroacetic acid and the like are used. As the solvent, any solvents can be used insofar as they are
not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane or chloroform, alcohol solvents such asmethanol or ethanol, diethyl ether, tetrahydrofuran and the like. The reaction temperature is -20°C or reflux temperature of the solvent.
Aromatization is achieved in the similar condition as that of Sonogashira coupling.
In the case where U is a nitrogen atom, the nitrogen atom may be protected with a suitable protective group in the similar manner as is protection of 1-position, and finally protected. Protection and deprotection of 1-position of pyrazole and U are achieved in the manner as described in Production method 3. Production method 32
(Formula Removed)
Compound 74, which already has a substituent introduced to U2 of compound 72 before subjected to cyclization can be readily produced by introducing a hetero group having an active methylene at α-position into 5(3) -position of compound 73 which is readily synthesized in accordance with the known method (for example, Synlett. 2000, 8, 1115-1118.), followed by cyclization.
As a reagent for introducing a hetero atom having a active methylene into compound 73, t-butoxycarbonyl glycine,
thioglycolic acid or its ester, thioglyconitrile, glycolic acid or its ester, glyconitrile and the like can be exemplified. Examples of the base to be used include, but are not limited to, sodium hydroxide, potassium hydroxide, sodium alkoxide, potassium alkoxide and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and preferred examples of such solvent include, but are not limited to, alcohols such as methanol, ethanol, isopropanol or tert-butanol. The reaction temperature is from room temperature to reflux temperature of the solvent.
In the case where U is an amino group, the amino group may be protected with a suitable protective group in the similar manner as is protection of 1-position, and protected in the final step. Protection and deprotection of 1-position of pyrazole and U are achieved in the manner as described in Production method 3. Production method 33
(Formula Removed)
Compound 77 can be produced in the following manner: 4-position of pyrazole of compound 75 which is easily synthesized in the documentarily known method (for example, J. Heterocycle. Chem. 1982, 1_9, 117.) is formylated by Vilsmeier reaction to render compound 7 6/ and then compound 7 6 is aromatized and
deprotected.
Vilsmeier reaction is achieved by a methylene iminium compound formed of phosphorus oxychloride and formamide, and as the formamide, N,N-dimethylformamide is preferred. The use amount of phosphorus oxychloride is from 1 to 2 equivalent(s) with respect to a starting material, and the use amount of N,N-dimethylformamide is from 1 equivalent with respect to the material to solvent amount. As the solvent, any solvents can be used without particular limitation insofar as the are not concerned with the reaction, and no solvent or N,N-dimethylformamide used as the formamide is preferred. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
As the method for aromatization, a documentarily known method can be used. For example, Lewis acids such as boron trifluoride-diethyl ether complex is used for the case of furan ring (for example, Synth. Commun. 1999, ,2_9, 729-747.), protonic acids such as trifluoroacetic acid or p-toluenesulfonic acid (for example, J. Org. Chem. 1998, 63, 2909-2917.) and the like can be used depending on the type of the hetero atom. In the case of a thiophene ring, the method of converting a hydroxyl group to a thiol group by means of Lawesson reagent (for example, J. Org. Chem. 1998, _63_, 2909-2917.), the method of conducting ring-closing after bromination of hydroxyl group (for example, J. Heterocycle. Chem. 1998, 35, 71-75.) and the like can be used. Bromination of hydroxyl group can be achieved with phosphorous
tribromide, 48% hydrobromic acid and the like. In the case of a pyrrole ring, a method of oxidizing hydroxyl group to aldehyde to obtain dialdehyde, and then closing the ring (for example, Tetrahedron, 1979, 35 1433.) , and the method of making the diol obtained by reduction of f ormyl group into a dibromide compound, and then closing the ring (for example, Synthesis, 1975, 252.) and the like can be used. Oxidation of hydroxyl group into aldehyde can be achieved using manganese dioxide or the like. Reduction of formyl group into alcohol can be achieved by sodium borohydride or the like, anddibrominationof diol can be achieved by phosphorous tribromide, 48% hydrobromic acid and the like. Protection and deprotection of 1-position of pyrazole can be achieved in the manner as described in Production method 3.
Production method 34
(Formula Removed)
Compound 81 can be produced by using compound 78 readily synthesized in the known manner (U=hydroxyl group: for example, J. Chem. SOC., Perkin Trans., 1985, 81., U=amino group: for example, J. Am. Chem. Soc. 1950, 72, 2978.) as a startingmaterial.
More specifically, 5(3) position of pyrazole of compound 78 is turned to a metal aryl by means of alkyl lithium, lithium amide or the like, followed by iodization or bromination to obtain compound 79, to which trimethylsilyl acetylene is coupled by Sonogashira coupling. Then detrimethylsilylation is conducted and the resultant compound 80 is aromatized and deprotected, whereby compound 81 is produced. In the case where U is a thiol group, after converting the amino group into diazonium using sodium nitrite ester or the like by way of the known method (Org. Synth. 1955, III, 809.) or the like, the resultant compound is treated with potassium O-ethyl dithiocarbonate or the like, whereby compound 81 is produced. Alternatively, compound 81 may be produced using a known method (J. Heterocycle. Chem. 1991, 28, 41.) or the like, by introducing a thiol group after halogenating 4-position of pyrazole with N-iodosuccinimide, N-bromosuccinimide or the like.
Conversion of compound 78 into a metal aryl and subsequent iodization or bromination are conducted in accordance with Production method 1, and conversion from compound 7 9 to compound 80, that is, Sonogashira coupling and subsequent detrimethylsilylation are conducted in accordance with Production method 31. Also, protection and deprotection of 1-position of pyrazole are achieved in the manner described in Production method 3.
In the general synthesis methods exemplified in Production method 35 to Production method 44 below, R1 has the same meaning
as the formula - (CO) h- (NRa)J,- (CRb=CRc) k-Ar in the general formula (I). These general synthesis methods are given as detailed description for conversion of R1 moiety, and the present invention is not limited to the exemplified Production methods . Therefore, a process of introduction and conversion of V may be included in any reaction route, and protecting a pyrazole ring in a desired production, step will not influence on the process. Production method 35
(Formula Removed)
The compound (I)-a representedby (h=j=0,k=l) in the general formula (I) can be produced after converting compound 82 into compound 84 by halogenating 3-position of compound 82 while protecting 1-position, compound 85 is obtained by Heck reaction or Suzuki reaction, followed by deprotection.
3-position of compound 82 is halogenated in the manner as described in Productionmethod 3, whereby compound 83 is obtained. As a protective group for 1-position of pyrazole in compound 83, tert-butoxycarbonyl group, trityl group and the like are preferred without limitation, and compound 83 can be led to
compound 84 in accordance with Production method 3.
By conducting Heck reaction or Suzuki coupling on compound 84 in the manner as described in Product ion method 3, it is possible to produce compound 85.
Deprotection of compound 85 readily proceeds by acid treatment in accordance with Production method 3, whereby compound (I)-a is produced. Production method 36
(Formula Removed)
Compound (I) -b wherein positional relationship between Ar and pyrazole is "cis" which is different from (I) -a can be produced by using a boronic acid which corresponds to a position isomer different from the boronic acid used in Production method 35. Starting from compound 84 and through compound 86, (I)-b can be produced in the similar manner as described in Production method 35. Production method 37

(Formula Removed)
With regard to (I)-c represented by (h=j=0, k=l, Rb=Rc=H) in the general formula (I), it is possible to construct the R1 moiety stepwise in the methods described in Production methods 35 and 36. That is, after introducing a vinyl unit and a vinylboronic acid unit into compound 84, aromatic ring coupling and deprotection are conducted in accordance with the Production method 3, whereby II)-c is produced.
Introduction of a vinyl group into compound 84 can be achieved, for example, by Stille reaction, Negishi reaction or Heck reaction. As a vinylation reagent, commercially available reagent is purchased and commercially unavailable reagents are prepared in a commonly used method. As the reaction reagent, vinyl trialkyl tin for Stille reaction, vinyl zinc halide for Negishi reaction, and ethylene gas for Heck reaction can be used. As a reagent other than the above, vinyltrialkoxysilane, vinyltrialkylsilane and the like can be exemplified.
Production of compound 88 into which vinyl boronic acid or vinylboronic acid ester unit is introduced can be achieved, for example, by Heck reaction with respect to compound 84. As the reaction reagent, vinylboronic acid pinacol ester or the like is exemplified without limitation.
With respect to compound 87, an aromatic ring can be introduced by Suzuki coupling with aryl boronic acid, Heck reaction with aryl halide, and Stille reaction with aryltrialkyl tin. With respect to compound 88, an aromatic ring can be introduced by Suzuki coupling with aryl halide.
As a Pd catalyst used in these coupling reactions, for example, tris(dibenzylideneacetone) dipalladium (0), reagents described in Production method 3 and the like are used without limitation, and a suitable phosphine ligand as described in Production method 3 may coexist. The condition for coupling reaction is achieved in accordance with Production method 3.
Furthermore, production of compound 88 into which vinylboronic acid unit is introduced can be achieved also by addition reaction of borane with respect to compound 87 in the presence of a rhodium or iridium catalyst. Examples of such reagent include, but are not limited to,
chloro(1,5-cyclooctadiene) rhodium (I) dimer and pinacol borane.
Production method 38
(Formula Removed)
(I)-c and (I)-d represented by (h=j=0, k=l, Rb=Rc=H) in the general formula (I) can be produced by converting compound 84 into alkynyl compound 89 by Sonogashira reaction, and reducing compound 89 to olefin.
The acetylene derivative used for Sonogashira reaction is readily prepared by conducting Sonogashira reaction using commercially available aryl halide and trimethylsilyl acetylene, and removing the trimethylsilyl group by an acid. It is possible to produce compound 89 from compound 84 in the manner as described in Production method 31.
In reduction from alkyne compound 89 to olefin, it is possible to control the generation ratio of position isomers by selecting a variety of reducing agents. As is exemplified in Production method 38, by reducing compound 89 using lithium aluminum hydride, Lindlar catalyst and the like under different conditions, it is possible to obtain trans (I)-c and cis (I)-d olefins as main products in the respective conditions. By
deprotection in accordance with Production method 3, (I)-c and (I)-d can be obtained. Production method 39
(Formula Removed)
(I)-e represented by (h=j=0,k=l,Rb=H) in the general formula (I) can be produced by converting aldehyde 90 into pyrazole 91, then converting a methyl group at 3-position into a methyl alcohol and then into a f ormyl group, and then conducting Wittig reaction. Conversion of aldehyde 90 to pyrazole 91 can be achieved, for example, by causing methyl metal such as methyl lithium or methyl magnesium to act on aldehyde 90, oxidizing the resultant alcohol in accordance with Production method 1, and then causing hydrazine to act. Conversion from compound 91 to compound 92 can be achieved, for example, by introduction of a halogen, followed by hydrolysis by treatment with a base. Introduction of halogen can be conducted in the similar manner as described in Productionmethod 3, and as is necessary, pyrazole may be protected. Conversion of halogen compound to alcohol 92 may be direct conversion using, for example, sodiumhydroxide,
potassium hydroxide and the like, however, it is also possible to obtain compound 92 by first converting into an acetate by action of sodium acetate, potassium acetate or the like, and then hydrolyzing the acetate sodium hydroxide, potassium hydroxide or the like. Also, it is possible to produce compound 93 by oxidizing the methyl group at 3-position of pyrazole of compound 91 with a suitable oxidizing reagent to an aldehyde. Oxidation from alcohol compound 92 to aldehyde 93 can be conducted in accordance with Production method 1. Wittig reaction of compound 93 can be achieved by reaction between compound 93 and phosphonium ylide. The phosphonium ylide can be readily synthesized by letting a commercially available alkyl halide or the like react with triphenylphosphine in an ether solvent to render a phosphonium salt, which is then treated with a base such as sodium hydride, sodium hydroxide, potassium carbonate or metal alkoxide. As the reaction solvent for Wittig reaction of compound 93, for example, ether solvents such as diethyl ether or tetrahydrofuran, hydrocarbon halides such as dichloromethane or chloroform, hydrocarbon solvents such as benzene or toluene, alcohol solvents such as methanol, as well as N, N-dimethylformamide and the like are used. The use amount of the base is usually from 1 to 3 equivalent (s) with respect to the material. The reaction temperature is from 0°C to reflux temperature of the solvent. Production method 4 0
(Formula Removed)
Compound (I)-f represented by (h=j=0,k=l) in the general formula (I) is obtained by letting a metal reagent such as alkyl lithium or aryl lithium act on aldehyde 93 to convert it an alcohol, oxidizing the resultant alcohol to ketone 94 in accordance with Production method 1, and then subjecting the ketone 94 to Wittig reaction in the similar manner as described in Production method 39.
Production method 41
(Formula Removed)
Compound 93 which is a starting material for synthesis in
Production method 39, Production method 40 or the like can be
produced, for example, by lithionating 1-position of halogen
compound 83 with N-butyllithium or phenyl lithium, lithionating
a halogen at 3-position with sec-butyllithium or
tert-butyllithium, and then letting the resultant compound react
with N, N-dimethylformamide, N-formylpiperidine,
methylphenylformamide or the like. The amount of
N-butyllithium or phenyl lithium is from 1 to 2 equivalent (s)
with respect to the material. The amount of sec-butyllithium
or tert-butyllithium is from 1 to 2 equivalent (s) with respect to the material. The amount of formylation reagent such as N, N-dimethylformamide is from 1 to 5 equivalent (s) with respect to the material. The reaction solvent is preferably ether solvents such as diethyl ether or tetrahydrofuran. The reaction temperature is from -78°C to room temperature. The reaction may be conducted after protecting pyrazole in the similar manner as described in Production method 1 as is necessary. In this case, the use amount of lithionating reagent is from theoretical amount to 2 equivalent(s).
Formyl compound 93 may be produced by subjecting compound 82 to Vilsmeier reaction in the similar manner as described in Production method 33. Production method 42
(Formula Removed)
Compound (I)-g represented by (h=l,j=k=0) in the above general formula (I) can be produced by letting compound 93 and metal aryl or metal halogenoaryl react in accordance with Production method 1 tomake alcohol 95, and oxidizing the alcohol to ketone in accordance with Production method 1.
Compound (I) -g may also be produced by introducing a suitable protective group to 1-position of compound 93, executing the above Production method, and the conducting deprotection.
Production method 43
(Formula Removed)
Compound (I)-h represented by (h=j=l,k=0) in the above general formula (I) can be produced by protecting 1-position of compound 93, oxidizing formyl group to convert into carboxylic acid 97, and then successively conducting amidation and deprotection.
Introduction of protective group into 1-position of compound 93 can be conducted in accordance with Production method 3. As the oxidizing reagent for oxidizing compound 96 into carboxylicacid 97, Jones reagent, pyridiniumdichromate, sodium chlorite can be exemplified. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include hydrocarbon halides such as dichloromethane or chloroform, as well as ethyl acetate, N,N-dimethylformamide, dimethyl sulfoxide and the like. The reaction temperature is from 0°C to reflux temperature of the solvent.
Amidation of carboxylic acid 97 can be achieved in accordance with Production method 47. Also amidation of carboxylic acid 97 can be achieved by converting carboxylic acid 97 into an acid chloride using thionyl chloride, oxalyl chloride and the like, and then subjecting the acid chloride to Schotten-Baumann
reaction with an amine in the presence of a base such as
triethylamine, diisopropylethylamine, pyridine and the like.
The protective group at 1-position is deprotected in
accordance with Production method 3, whereby (I) -h is produced.
Production method 44
(Formula Removed)
Carboxylic acid 97 produced in Production method 43 can also be produced by hydrolyzing compound 98, and introducing a protective group at 1-postion after diazotization and reduction.
For hydrolysis of compound 98, for example, an aqueous solution of sodium hydroxide, an aqueous solution of potassium hydroxide and the like is used. The reaction for converting into diazonium salt is achieved by reaction with a nitrite ester such as sodium nitrite ester or isoamyl nitrite ester in the presence of an acid. As the reaction solvent, for example, alcohol solvents such as methanol or ethanol, water and the like are used, and as the acid, hydrochloric acid, sulfuric acid, acetic acid and the like are can be used. The reaction temperature is usually around 0°C.
Reduction of diazonium salt and subsequent ring-closing of the indazole ring can be achieved by action of a reducing reagent, such as tin chloride (II) or copper chloride (II), for

example in the presence of an acid, and the use amount of these reagent is usually from 1 to 10 equivalent (s) with respect to the material. In general, the ring-closing occurs spontaneously in the reaction system accompanied with dehydration, and thereby compound 99 is obtained. As the reaction solvent, for example, alcohol solvents such as methanol or ethanol, as well as hydrochloric acid, sulfuric acid, acetic acid and the like can be used. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
Introduction of protective group into 1-position of compound 99 can be achieved in accordance with Production method 3 . Also compound 97 can be produced by hydrolysis of ester under a usual condition after converting a carboxylic acid into an ester in a usually-used condition, and introducing a protective group into 1-position as is necessary.
The following production methods are concrete description for typical functional group conversion after formation of condensed pyrazole rings represented by the general formulae (I) (II) and (III) . It is to be noted that following production methods may also be employed in production steps before formation of pyrazole ring rather than after formation of condensed pyrazole ring, and commercially available starting materials and intermediates originally having suitable functional groups may be used. Diversity of functional groups to be introduced is not limited to the methods exemplified in Production methods. Production method 45
(Formula Removed)
In general, aryl halide can be converted into a cyano group by means of a cyanizing reagent. As the halogen atom T3, a chlorine atom, bromine atom and iodine atom are recited, with a bromine atom or iodine atom being desired. In the case where the substitution position of halogen atom corresponds to ortho-position or para-position of a nitrogen atom forming the aromatic ring, the halogen may be a chlorine atom. Although a protective group for pyrazole ring is sometimes unnecessary, it is usually preferable to have a protective group. As the cyanization reagent to act on compound 100, zinc cyanide, lithium cyanide, sodium cyanide, potassium cyanide and the like are exemplified, and the cyanization is achieved by using a transition metal catalyst such as tetrakis (triphenylphosphine) palladium, tris(dibenzylideneacetone) dipalladium, dichlorobis(triphenylphosphine) palladium, palladium diacetate and the like, and adding a catalyst amount of a copper iodide or a phosphine ligand such triphenylphosphine and 1,1'-bis(diphenylphosphino) ferrocene as is necessary for promoting the reaction. As the solvent to be used, dimethylformamide, N-methylpyrrolidone, propionnitrile, acetonitrile and the like are preferred. The reaction temperature is preferably in the range from 80°C to 150°C. Furthermore, nitrile 101 may also be produced by letting copper
cyanide and compound 100 react in a solvent such as dimethylformamide or N-methylpyrrolidone at a temperature ranging from 140°C to 200°C. Production method 4 6
(Formula Removed)
Hydrolysis of nitrile of compound 102 which is readily produced by deprotection of compound 101 or the like can be achieved by using an acid or alkaline. As the acid, hydrochloric acid, hydrous sulfuric acidand the like canbe recited. Although the reaction may be conducted in the absence of solvent, when a solvent is used, for example, methanol, ethanol, propanol and the like alcohol solvents, as well as acetic acid and the like can be used. The reaction temperature is usually from room temperature to reflux temperature of the solvent. As the alkaline, for example, sodium hydroxide, potassium hydroxide and the like can be used. As the solvent, the reaction may be conducted just in alkaline water, however, when a solvent is used, for example, methanol, ethanol, propanol and the like alcohol solvents, dioxane and the like can be used. The reaction temperature is usually from room temperature to reflux temperature of the solvent. In this production method, when compound 101 having tert-butoxycarbonyl group, trityl group or the like, for example, as a protective group at 1-position is
subjected to the similar hydrolysis condition, deprotection generally occurs to generate 103. Production method 47
(Formula Removed)
Amidation of carboxylic acid 103 can be achieved by mixing amine and a condensing reagent. As the condensing reagent, for example, dicyclohexylcarbodiimide, diisopropylcarbodiimide, l-ethyl-3-(3-dimethylaminopropyl) carbodiimide, l-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and the like can be used. As is necessary,
1-hydroxybenzotriazole, N—hydroxysuccinimide and the like may be added. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include halogen solvents such as dichloromethane or chloroform, ether solvents such as ether or tetrahydrofuran, as well as ethyl acetate, N,N-dimethylformamide, N-methylpyrrolidone, toluene and the like. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
Compound 104 may be produced by introducing a suitable protective group into 1-position of compound 103, executing the above Production method, and then conducting deprotection. Production method 48
(Formula Removed)
For converting a carboxyl group of compound 103 into other
functional groups such as amino group, for example, by Curtius
rearrangement, it is preferred to introduce a protective group
into the pyrazole ring as shown in Production method 48 as is
necessary, thereby converting into compound 105. A variety of
protective groups described in the Production method 3 can be
used, and a trityl group or the like is preferred. Any bases
can be used as the base, and sodium hydride or the like is preferred.
The use amount is from 2 to 3 equivalents. As the reagent,
tritylchloride is preferably used in the amount of from 1 to
2 equivalent (s) . As the solvent, any solvents canbe used insofar
as they are not concerned with the reaction, and preferred
examples of such solvent include, but are not limited to, ether
solvents such as tetrahydrofuran or dioxane,
N,N-dimethylformamide and the like. The reaction temperature
is usually ice-cooled temperature to reflux temperature of the
solvent.
Production method 4 9
(Formula Removed)
After converting a carboxyl group of condensed pyrazole
ring compound 105 into a carbamate by Curtius rearrangement, the carbamate is removed, to thereby obtain compound 106. Curtius rearrangement of compound 105 can be achieved, for example, by making an isocyanate using diphenylphosphorylazide and an amine such as triethylamine or diisopropylethylamine, and letting the resultant isocyanate react with an alcohol; or making an acid chloride using thionyl chloride, oxalyl chloride or the like, converting the resultant acid chloride into an isocyanate using lithium azide, sodium azide, potassium azide and the like, and letting the resultant isocyanate react with an alcohol. Preferred examples of the alcohol include, but are not limited to, benzyl alcohol or tert-butanol. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of which include, but are not limited to, toluene, benzene, tetrahydrofuran, dioxane and the like. Alternatively, the reaction may be conducted using a solvent amount of an alcohol such as tert-butanol. Usually the reaction temperature is from room temperature to reflux temperature of the solvent. Deprotection of carbamate, for example, deprotection of tert-butoxycarbonyl group is readily achieved by means of acid in accordance with Production method 3. Deprotection of benzyloxycarbonyl group is readily achieved by hydrogen addition.
Amidation of amine 106 can be achieved by mixing a carboxylic acid and a condensing reagent. The condensation condition is as described in Production method 47.
In the case where compound 106 is a nitrogen-containing aromatic amine, specifically, amidation of compound 22 or the like can be achieved by the method using the condensing reagent as described above, and preferably by a method of letting acid chloride or acid anhydride react in the presence of a base. Examples of the base include, but are not limited to, triethylamine, diisopropylethylamine, pyridine and the like. As the solvent, any solvent can be used insofar as they are not concerned with the reaction, and examples of such solvent include halogen solvents such as dichloromethane or chloroform, ether solvents such as ether or tetrahydrofuran, as well as ethyl acetate, toluene and the like. The reaction temperature is usually from -78°C to reflux temperature of the solvent. For amidation of nitrogen-containing aromatic amine, dehydration condensation using triphenylphosphine and bromotrichloromethane is also possible. Production method 50
(Formula Removed)
In general, aryl halide can be converted into an amino group by coupling reaction using a palladium catalyst. As the halogen atom T3 in compound 100, bromine atom and iodine atom are represented, with bromine or iodine atom having high reactivity being preferred. Also in an aromatic ring wherein substitution position of halogen atom is ortho-position or para-position of
the nitrogen atom constituting the ring, T3 may be a chlorine atom. Furthermore, the protective group of pyrazole ring is sometimes unnecessary, however, it is usually preferred to have a protective group.
As a palladium catalyst used for amination of compound 100, for example, tris (dibenzylideneacetone)dipalladium, palladium diacetate and the like are used, as the phosphine ligand, 2,2'-bis(diphenylphosphino)-1,1'-naphthyl, 1,1'-bis(diphenylphosphino) ferrocene,
tri (tert-butyl) phosphine and the like are used, and as the base, sodium tert-butoxide, potassium tert-butoxide, cesium carbonate and the like are used. As an ammonia equivalent, benzophenoneimine is preferred without limitation. As the acid used for hydrolysis of the resultant imine compound, diluted hydrochloric acid, diluted sulfuric acid and the like can be used without limitation. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, toluene, tetrahydrofuran, dioxane, dimethoxyethane and the like. Usually the reaction temperature is from room temperature to 120°C. As a result of this, it is possible to produce amine 106.
Production method 51
(Formula Removed)
In general, it is possible to readily introduce a variety of functional groups into aryl halide by coupling using Pd(0). For example, as exemplified in Production method 51, it is possible to introduce an acyl group by coupling, to thereby obtain corresponding acyl compound 108. As the halogen atom T3, chlorine atom, bromine atom and iodine atom are recited, with bromine or iodine atom being desired. Furthermore, in an aromatic ring wherein substitution position of halogen atom is ortho-position or para-position of the nitrogen atom constituting the ring, T3 may be a chlorine atom. Furthermore, the protective group of pyrazole ring is sometimes unnecessary, however, it is usually preferred to have a protective group. As the method for introducing an acetyl group, Stille coupling using tributyl(1-ethoxyvinyl)tin can be recited. As the tin reagent, commercially available one was purchased. The use amount of tributyl (1-ethoxyvinyl) tin is f roml to 3 equivalent (s) with respect to the material. As the catalyst to be used, for example, tetrakis(triphenylphosphine) palladium(O) and the like is preferred without limitation. The use amount of catalyst is about 5% by mole with respect to the material. As the solvent, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, tetrahydrof uran, dioxane, N, N-dimethylf ormamide, diethyleneglycol dimethyl ether, toluene, xylene and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent. The vinyl ether which is
obtainable as an intermediate is readily hydrolyzed with acid, to be led to a ketone. As such an acid, diluted hydrochloric acid or the like is preferred without limitation. Depending on the condition, deprotection may be conducted as exemplified in Production method. It is also possible that N-bromosuccinimide is caused to act instead of the acid to be acted on vinyl ether, thereby introducing a bromomethylketone unit. Production method 52
(Formula Removed)
As another way to conversion with respect to substituent V, for example, as exemplified in Production method 52, a cyano group of compound 102 may be reduced so as to convert to aralkyl amine 109. Although a protective group is not particularly required, the reaction may be conducted for compounds having protective groups introduced therein.
As the reducing reagent of cyano group, for example, sodium borohydride, lithium aluminum hydride, aluminum hydride and the like are recited. As is necessary, additives such as aluminum trichloride, boron trifluoride, cobalt chloride, Raney nickel and the like may be added. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, ether solvents
such as diethyl ether, tetrahydrofuran, dioxane,
dimethoxyethane and the like. The reaction temperature is
usually from -78°C to reflux temperature of the solvent.
Production method 53
(Formula Removed)
Compound 111 can be produced by subjecting compound 100 to Suzuki coupling or Stille coupling, followed by deprotection. Furthermore, although the protective group of pyrazole ring is sometimes unnecessary, it is usually preferred to have a protective group.
The aryl bronic acid used for Suzuki coupling or aryl trialkyl tin used for Stille coupling is commercially available, or readily prepared in accordance with Production method 3 if not commercially available. The use amount of aryl boronic acid used for coupling reaction, or aryl trialkyl tin is from 1 to 3 eguivalent(s) with respect to the material. As the solvent to be used, for example, palladium acetate (II), dichlorobistriphenyl phosphine palladium (II), tetrakis(triphenylphosphine) palladium(O) and the like can be exemplified. The use amount of catalyst is about 5% by mole with respect to the material. As is necessary, twice as much as catalyst by mole of phosphine ligand, for example, tri-tert-butylphosphine, 2-(di-tert-butylphosphino) biphenyl, 2-(dicyclohexylphosphino) biphenyl, triphenyl phosphine and
the like may be added. As the base to be used, sodium hydrogen
carbonate, sodium carbonate, potassium carbonate, cesium
carbonate, potassium fluoride and the like can be recited. As
the solvent, any solvents can be used insofar as they do not
inhibit the reaction, and examples of such solvent include, but
are not limited to, N,N-dimethylf ormamide, N-methylpyrrolidone,
tetrahydrofuran, dioxane, diethyleneglycol dimethyl ether,
toluene and the like. The reaction temperature is usually from
room temperature to reflux temperature of the solvent.
Deprotection of compound 110 is conducted in accordance
with Production method 3.
Production method 54
(Formula Removed)
Compound 110 obtained by Production method 53 can also be produced by converting compound 100 into a boronic acid, and conducting Suzuki coupling.
Boronic acid 112 can be obtained by converting compound 100 into aryl lithium, letting the aryl lithium react with trialkyl borate to lead a borate ester, and then hydrolyzing the borate ester. As the alkyl lithium for converting compound 100 into aryl lithium, for example, N-butyllithium, sec-butyllithium, tert-butyllithium, phenyl lithium and the like are used, and additives such as N,N,Nr N-tetramethylethylenediamine or
hexamethylphosphoramide may be added as necessary. Hydrolysis after making borate ester by letting aryl lithium and trialkyl borate react with each other may be achieved by adding water or using an acid such as hydrochloric acid of sulfuric acid. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and preferred examples of such solvent include, but are not limited to, diethyl ether, tetrahydrofuran, dioxane, dimethoxyethane and the like ether solvents. The reaction temperature is from -78°C to room temperature.
Compound 110 can be produced by coupling boronic acid 112 and aryl halide or aryl sulfonates by Suzuki coupling in accordance with Production method 53.
Production method 55
(Formula Removed)
Compound 110 obtainedby Productionmethod 53may be produced
by converting compound 100 into tin compound 113, and then conducting Stille coupling.
Tin compound 113 can be produced by converting compound 100 into aryl lithium in accordance with Production method 54, and then letting the aryl lithium react with chlorotrialkyl tin or hexaalkyl ditin.
Compound 110 can be produced by Stille coupling between
tin compound 113 and aryl halide or aryl sulfonates in accordance with Production method 53. Production method 56
(Formula Removed)

Compound 114 can be produced by letting compound 101 produced in Production method 45 to react with hydrazide after deprotecting the same.
Deprotection of compound 101 is conducted in accordance with Production method 3.
The hydrazide used for the reaction with compound 102 is purchased if commercially available, or can be readily prepared if not commercially available. Compound 102 can also be produced by amide condensing a carboxylic acid and a mono-protected hydrazine, and then conducting deprotection. As the reaction solvent, any solvents can be usedinsofarastheyNare not concerned with the reaction, and examples of such solvent include, but are not limited to, ethanol, toluene, xylene, dimethylformamide, N-methylpyrrolidone, diphenyl ether and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Production method 57
(Formula Removed)
compound 114 produced by Production method 56 can also be produced by converting compound 102 into an imidate, then reacting the imidate with hydrazide.
As the acid used for converting compound 102 into imidate 115, hydrogen chloride, hydrogen bromide, sulfuric acid and the like can be exemplified. As the alcohol, methanol, ethanol and the like are preferred without limitation. As the solvent, any solvents can be used without limitation insofar as they are not concerned with the reaction, and dichloromethane, 1, 4-dioxane, diethyl ether, toluene or the like is used, or alternatively the alcohol to be reacted may be used as a solvent. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
From compound 115 and hydrazide, it is possible to produce compound 114. A base may be added if necessary. As the base to be used, triethylamine, diisopropylethylamine, potassium carbonate and the like can be recited. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and preferred examples of such solvent include, but are not limited to, methanol, ethanol, tetrahydrofuran, toluene, 1,4-dioxane, dimethylformamide and the like. The reaction temperature is usually from room temperature to reflux
temperature of the solvent. Production method 58
(Formula Removed)
Compound 114 produced by Production method 56 can also be produced by converting compound 102 into a thioimidate and then letting the thioimidate react with hydrazide.
Thioimidate 116 can be produced by adding a thiol to compound 102 in the presence of an acid catalyst. As the acid to be used, hydrogen chloride, hydrogen bromide, sulfuric acid and the like canbe recited. As the thiol to be used, forexample, ethanethiol, propanethiol, thiophenol and the like can be recited without limitation. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ethanol, dichloromethane, toluene, diethyl ether, dioxane, dimethoxyethane and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
In accordance with Productionmethod 57 , by letting compound 116 react with hydrazide, compound 114 can be produced. Production method 59
(Formula Removed)
Compound 114 producedby Productionmethod 56 canbe produced by converting compound 101 produced by Production method 45 into thioamide 117, letting thioamide 117 to react with alkyl halide to render it thioimidate 118, then letting the thioimidate 118 react with hydrazide, and conducting deprotection.
Thioamidation of compound 101 is conducted using the condition which is usually employed, for example, using hydrogen sulfide and a base. In this case, as the base, triethylamine, diisopropylethylamine or the like is used, and as the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, pyridine and the like. The reaction temperature is from room temperature to reflux temperature of the solvent.
As the alkyl halide to be used for thiomidation of thioamide 117, methyl iodide or the like is preferred without limitation. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, dichloromethane, acetonitrile,
toluene, tetrahydrofuran, 1,4-dioxane, dimethylformamide and the like. The reaction temperature is from room temperature to reflux temperature of the solvent.
In accordance with Production method 58, thioimidate 118 and hydrazide are reacted, followed by deprotect ion in accordance with Production method 3, to thereby produce compound 114.
Production method 60
(Formula Removed)
Compound 119 produced by Product ion method 59 can be produced by esterifying compound 105 produced by Production method 48, letting the resultant ester react with hydrazine to render it hydrazide, and letting the hydrazide react with imidate or thioimidate.
Esterif ication of compound 105 canbe achieved, for example, by dehydration reaction with alcohol using an acid catalyst, dehydration reaction with alcohol using a condensing reagent, and reaction using diazomethane. For example, in the case where a sulfuric acid is used as the acid catalyst, methanol, ethanol or the like is preferably used as the alcohol. And the solvent is preferably the alcohol to be used. The reaction temperature
is usually from 0°C to reflux temperature of the solvent. As the reaction solvent in the case of using diazomethane, for example, diethyl ether, methanol, tetrahydrofuran or the like is used, and the reaction temperature is usually from 0°C to room temperature.
As the solvent to be used for reaction between ester 120 and hydrazine, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ethanol, 1,4-dioxane, toluene, N-methylpyrrolidone, N,N-dimethylformamide and the like. The reaction temperature is from room temperature to reflux temperature of the solvent.
As the solvent to be used in the reaction between hydrazide 121 and imidate or thioimidate, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ethanol, 1,4-dioxane, dichloromethane, toluene, N-methylpyrrolidone, N,N-dimethylformamide and the like, and if necessary, a base such as triethylamine, potassium carbonate or the like may be added. The reaction temperature is from room temperature to reflux temperature of the solvent. The imidate to be used is purchased if commercially available, or can be produced, for example, by causing nitrile to react with an alcohol such as ethanol in the presence of an acid such as hydrogen chloride if not commercially available. The thioimidate to be used is purchased if commercially available, or can be produced, for
example, by causing thioamide to react with an alkyl halide or causing a nitrile to react with ethanethiol or thiophenol in the presence of an acid such as hydrogen chloride if not commercially available.
Production method 61
(Formula Removed)
Carboxylic acid 105 produced by Production method 48 can also be produced by converting compound 100 into an aryl lithium in themanner as described in Productionmethod 54 , and the letting the aryl lithium react with carbon dioxide. Production method 62
(Formula Removed)
Hydrazide 121 produced by Production method 60 can also be produced by amide-condensing carboxylic acid 105 produced by Production method 4 8 and a mono-protected hydrazine, and then conducting deprotection.
Amidation of carboxylic acid 105 can be achieved by mixing amono-protectedhydrazine anda condensing reagent in accordance with Production method 47. As a protective group of
mono-protected hydrazine, for example, tert-butyloxycarbonyl
group, benzyloxycarbonyl group and the like can be recited.
As the condition for deprotecting compound 122, in the case
of tert-butyloxycarbonyl group, deprotection can be readily
achieved by using an acid in accordance with Production method
3. Similarly, in the case of benzyloxycarbonyl group,
deprotection can be easily achieved by catalytic hydrogen
reduction.
Production method 63
(Formula Removed)
Compound 119 produced by Production method 59 can also be produced by converting carboxylic acid produced 105 by Production method 48 into an acid chloride, letting the acid chloride react with a thiocyanate salt, letting the resultant compound 124 react with a nucleophilic reagent to render it compound 125, and then letting compound 125 react with hydrazine.
As themethod f orproducing acid chloride 123 from carboxylic acid 105, usual condition is employed, and for example, thionyl chloride or oxalyl chloride is used, and as if necessary, a small
amount of N,N-dimethylformamide is added. As the solvent, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, dichloromethane, toluene, tetrahydrofuran, 1,4-dioxane and the like. Also the reaction may be conducted in the absence of solvent. The reaction temperature is usually from 0 °C to reflux temperature of the solvent.
As the solvent used in producing compound 124 from acid chloride 123 and thiocyanate salt, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, toluene, acetonitrile, pyridine and the like. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
As the nucleophilic reagent used in producing compound 125 from compound 124, alcohol and amine, alkyl lithium, Grignard reagent and the like can be recited. As the solvent to be used when the nucleophilic reagent is alcohol or amine, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, toluene, acetonitrile, pyridine and tetrahydrofuran. The reaction temperature is usually from 0°C to reflux temperature of the solvent. Also the reaction may conducted in the absence of solvent. As the solvent to be used when the nucleophilic reagent is alkyl lithium or Grignard reagent, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, diethyl ether,
tetrahydrofuran, dimethoxyethane and the like. The reaction temperature is usually from -78°C to room temperature.
As the solvent to be used in the reaction between compound 125 and hydrazine, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, ethanol, methanol, pyridine, toluene, tetrahydrofuran and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Production method 64
(Formula Removed)
Compound 127 can be produced by letting α-aminoacetal or α-aminoketal react on imidate 115 produced by Production method 57 or thioimidate 116 produced by Production method 58 to render it 126, and conducting acid treatment on 126.
As the solvent to be used in producing compound 126 from compound 115 or 116, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, acetic acid, dimethoxyethane, dimethylformamide and the like, and if
necessary, a base such as triethylamine, diisopropylethylamine,
potassium carbonate or the like may be added. The reaction
temperature is usually from room temperature to reflux
temperature of the solvent.
As the acid used in acid treatment for compound 126, for
example, hydrochloric acid, sulfuric acid, acetic acid,
para-toluenesulfonic acid and the like can be recited. As the
reaction solvent, for example, methanol, ethanol, acetic acid,
1, 2-dimethoxyethane, N,N-dimethylformamide and the like can be
recited. The reaction temperature is usually from room
temperature to reflux temperature of the solvent.
Production method 65
(Formula Removed)
Compound 127 produced by Production method 64 can also be produced by introducing a protective group to compound 128 (compound in which V1 is a hydrogen atom in compound 127), lithionating the imidazole and letting an electrophilic reagent act on the lithio compound, and then conducting deprotection.
As the protective group to be introduced into compound 128,
for example, p-toluenesulfonyl group, dimethylsulfamoyl group, methoxymethyl group and the like can be recited. Introduction of p-toluenesulfonyl group and dimethylsulfamoyl group and methoxymethyl group can be achieved by letting compound 128 react with p-toluenesulfonyl chloride or dimethylsulfamoyl chloride or chloromethylmethyl ether in the presence of a base. Preferred examples of the base include, but are not limited to, triethylamine, 4-N/N-dimethylamionopyridine/ sodium hydroxide, sodium hydride, potassium tert-butoxide, potassium carbonate and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane, hydrocarbon halides such as dichloromethane or chloroform, as well as pyridine, acetonitrile, dimethyl sulfoxide, dimethylformamide, toluene and the like. The reaction temperature is usually from 0°C to reflux temperature of the solvent.
As the alkyl lithium for litionation of compound 129, for example, N-butyllithium, sec-butyllithium, tert-butyllithium, as well as phenyl lithium is used, and if necessary, an additive such as N,N,N',N'-tetramethylethylenediamine, hexamethylphosphoramide and the like may be added. As the lithium amide, for example, lithium diisopropylamide, lithium 2,2,6,6-tetramethylpiperidide or the like is used. As the electrophilic reagent to be used, for example, alkyl halide,
aldehyde, isocyanate and the like canbe recited. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, ether solvents such as diethyl ether, tetrahydrofuran, dioxane or dimethoxyethane, as well as benzene, toluene and the like. The reaction temperature is from -78°C to room temperature.
Deprotection of p-toluenesulfonyl group and dimethylsulfamoyl group is readily achieved by means of a base. As the base, sodium hydroxide water, potassium hydroxide water and the like can be recited without limitation. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, alcohol solvents such as methanol or ethanol, and ether solvents such as diethyl ether, tetrahydrofuran or dioxane, dimethoxyethane. The reaction temperature is room temperature or reflux temperature of the solvent. Deprotection of methoxymethyl group can be achieved by treating the remaining aminal with ammonia water after acid treatment. Deprotection of dimethylsulfamoyl group can be readily achieved by an acid. As the acid, hydrochloric acid, sulfuric acid, hydrobromic acid, perhydrochloric acid and the like can be recited. As the reaction solvent, any limitation insofar as they are not concerned with the reaction, and examples of such solvent include methanol, ethanol, water, dioxane and dimethoxyethane. The reaction temperature is room temperature or reflux temperature of the
solvent.
Production method 66
(Formula Removed)
Compound 127 produced by Production method 64 can also be produced by letting imidate 115 produced by Production method 57 or thioimidate 116 produced by Production method 58 react with ammonia to render it amidine 131, and then letting amidine 131 react with α-haloketone.
As the solvent to be used in producing 131 from compound 115 or compound 116, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, propanol, water, dioxane, dimethoxyethane and tetrahydrofuran. The reaction temperature is room temperature or reflux temperature of the solvent.
By letting amidine 131 and a-haloketone in the presence of a base, it is possible to produce 127. As the base to be used, for example, triethylamine, dimethylaminopyridine, sodium hydroxide, potassium carbonate, potassium tert-butoxide and the like can be recited. As the solvent, any solvents can
be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, dioxane, tetrahydrofuran, toluene, pyridine and N,N-dimethylformainide. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Production method 67
(Formula Removed)
Compound 134 can be produced by letting α-haloketone 132 produced by Production method 51 react with amidine, and then conducting deprotection.
By letting compound 132 and amidine react with each other in the presence of a base, it is possible to produce compound 133. As the base to be used, for example, triethylamine, dimethylaminopyridine, sodium hydroxide, potassium carbonate, potassium tert-butoxide and the like can be recited. As the solvent, any solvents canbeusedinsofarastheyare not concerned with the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, dioxane, tetrahydrofuran, toluene, pyridine and N,N-dimethylformamide. The reaction temperature is usually from room temperature to reflux
temperature of the solvent.
Deprotection of compound 133 is conducted in accordance with Production method 3. Production method 68
(Formula Removed)
Compound 139 can be produced by converting compound 135 produced as an intermediate in Production method 51 into diketone 137 by aldol reaction and subsequent oxidation reaction, then constructing a pyrazole ring by using hydrazine, and conducting deprotection.
As the base to be used in the aldol reaction for compound 135, for example, sodium hydroxide, sodium methoxide, lithium hexamethyldisilazide, lithium diisopropylamide and the like can be recited. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, diethyl ether, tetrahydrofuran, dimethoxyethane, toluene and dioxane. The
reaction temperature is usually from 0°C to reflux temperature of the solvent.
As the oxidizing reagent to be used in the oxidation reaction for compound 136, for example, sulfurtrioxide-pyridine complex, N-methylmorpholine-N-oxide, a variety of chromic acid oxidizing reagents and the like can be used, and also the oxidization may be achieved by Swern oxidation, Moffat oxidation and the like. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include hydrocarbon halides such as dichloromethane or chloroform, as well as ethyl acetate, acetonitrile, dimethyl sulfoxide, dimethylformamide and the like. The reaction temperature is usually from -78°C to reflux temperature of the solvent.
As the reaction solvent used for the reaction between compound 137 and hydrazine, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, tetrahydrofuran, dioxane, pyridine and acetic acid. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Deprotection of compound 138 is conducted in accordance with Production method 3. Production method 69
(Formula Removed)
Compound 138 produced by Production method 68 can also be
produced by converting compound 135 which is produced as an intermediate in Production method 51 into enamine 140, and then letting enamine 140 react with hydrazine.
Enamine 140 can be produced by letting compound 135 and dimethylamide-dimethylacetal react with each other. As the reaction solvent, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, toluene and dimethylformamide. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
As the reaction solvent to be used in the reaction between enamine 140 and hydrazine, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, tetrahydrofuran, dioxane, pyridine and acetic acid. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Production method 70
(Formula Removed)

Compound 142 can be produced by letting hydrazide 121 producedby Productionmethod 60 react with carbonyldiimidazole, and then conducting deprotection.
As the reaction solvent used in the reaction between hydrazide 121 and carbonyldiimidazole, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, tetrahydrofuran, dimethoxyethane and dimethylformamide. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Deprotection of compound 141 is conducted in accordance
with Production method 3.
Production method 71
(Formula Removed)
Compound 145 can beproducedby letting compound 101 produced by Production method 45 react with hydroxyammonium chloride in the presence of a base, to render it compound 143, letting compound 143 react with carbonyldiimidazole, and the conducting deprotection.
As the base to be used in production of compound 143, for example, triethylamine, potassium carbonate, sodium hydroxide and the like can be recited. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, methanol, ethanol, propanol, dioxane and N,N-dimethylf ormamide. The reaction temperature is room temperature or reflux temperature of the solvent.
As the solvent to be used in the reaction between compound 143 and carbonyldiimidazole, any solvents can be used insofar as they do not inhibit the reaction, and examples of such solvent include, but are not limited to, tetrahydrofuran, dimethoxyethane and dimethylformamide. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Deprotection of compound 144 is conducted in accordance with Production method 3. Production method 72

(Formula Removed)
Compound 149 can be produced by selectively protecting 1-posit ion after deprotecting compound 14 6 to make compound 14 8, introducing a substituent into a phenol group, and the removing the protective group. J in compound 146 is preferably a methyl group.
Deprotection of compound 14 6 is readily achieved by means of Lewis acid, alkaline metal salt of thio, acid and the like. As the Lewis acid, boron tribromide, aluminum trichloride and the like are used, as the alkaline salt of thiol, sodium salts of ethanethiol or thiophenol and the like are used, and as the acid, trifluoroacetic acid, hydrobromic acid and the like are used. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane, chloroform or carbon tetrachloride, as well as ethyl acetate, acetonitrile, dimethyl sulfoxide, N, N-dimethylformamide and the like. The reaction temperature is -20°C or reflux temperature of the solvent.
The subsequent selective protection of 1-position is achieved in the manner as described in Production method 3. Introduction of alkyl group into compound 148 can be achieved by reaction with a halide in the presence of a base. As the base to be used, sodium hydride, potassium carbonate, cesium
carbonate and the like are recited, and the use amount thereof is usually fro 1 to 2 equivalent (s) . As the halide to be used, bromides and iodides are preferred without limitation, and the use amount thereof is usually from 1 to 3 equivalent (s) with respect to the material. As the halide, both aliphatic halides and aromatic halides can be employed and they may have suitable functional groups. As for aromatic halides, iodides are particularly preferred, and by adding a metal catalyst such as copper iodide in the presence of a base, an excellent result is achieved. The use amount of metal catalyst is usually from catalyst amount to 1 equivalent. In the cases of highly-reactive aralkyl halides or aryl halides, the reaction can be achieved by using a salt such as sodium iodide instead of the base. As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, halogen solvents such as dichloromethane, chloroformor 1,2-dichloroethane, hydrocarbon solvents such as benzene or toluene, ether solvents such as tetrahydrofuran, and polar solvents such as N,N-dimethylformamide or acetonitrile. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
Furthermore, compound 148 can be converted into an ester by reaction with acid chloride or acid anhydride in the presence of a base. As the base, triethylamine, diisopropylethylamine, pyridine and the like can be recited without limitation. As
the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include halogen solvents such as dichloromethane or chloroform, ether solvents such as ether or tetrahydrofuran, as well as ethyl acetate, toluene and the like. The reaction temperature is usually from -78°C to reflux temperature of the solvent. Furthermore, compound 148 can also be esterified by mixing a carboxylic acid and a condensing reagent. As the condensing reagent, for example, dicyclohexylcarbodiimide, diisopropylcarbodiimide, l-ethyl-3-(3-dimethylaminopropyl) carbodiimide, l-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and the like can be recited.
1-Hydroxybenzotriazole, A7-hydroxysuccinimide and the like may be added where appropriate. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include halogen solvents such as dichloromethane or chloroform, ether solvents such as ether or tetrahydrofuran, as well as ethyl acetate, dimethylformamide, toluene and the like. The reaction temperature is usually from room temperature to reflux temperature of the solvent. Furthermore, compound 148 may be converted into an ester by reaction with a carboxylic acid under the condition of an acid catalyst. As the acid, hydrochloric acid, sulfuric acid, trifluoroacetic acid and the like are exemplified. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but
are not limited to, halogen solvents such as dichloromethane, chloroform or 1, 2-dichloroethane, hydrocarbon solvents such as benzene or toluene, ether solvents such as tetrahydrofuran, and polar solvents such as N,N-dimethylformamide or acetonitrile. The reaction temperature is usually from room temperature to reflux temperature of the solvent.
By deprotecting 1-position in the manner as described in Production method 3, it is possible to produce compound 149.
Production method 73
(Formula Removed)
Compound 148 obtained by Production method 72 can also be
produced by selectively deprotecting compound 14 6.
For selective deprotection of compound 146, for example, Lewis acids such as boron tribromide or aluminum trichloride, alkaline metal salts such as sodium salts of ethanethiol or thiophenol, and acids such as trifluoroacetic acid or hydrobromic acid are used, and alkaline metal salts of thiol are preferably used. As the solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, hydrocarbon halides such as dichloromethane, chloroform and carbon tetrachloride, as well as ethyl acetate, acetonitrile, dimethyl sulfoxide, N,N-dimethylformamide and the like. The reaction temperature is -20°C or reflux temperature of the solvent.
Production method 74
(Formula Removed)
Compound 14 9 can also be produced by letting compound 147 react with halide, carboxylic acid, acid chloride or acid anhydride in the similar manner as described in Production method 72. Production method 75
(Formula Removed)
Compound 151 can beproducedby letting compound 143 produced
by Production method 71 react with an acid chloride, allowing dehydrating-cyclization to render compound 150, and then conducting deprotection.
In producing compound 150 from compound 143, a base may be added. As the base to be used, for example, triethylamine, pyridine, sodium hydride and the like are recited. As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, tetrahydrofuran, dimethoxyethane and N,N-dimethylformamide. The reaction
temperature is room temperature or reflux temperature of the solvent. A corresponding acid anhydride or ester may be used instead of the acid chloride used for acylation.
Deprotection of compound 150 is conducted in accordance with Production method 3. Production method 76
(Formula Removed)
Compound 154 can be produced by letting compound 152 react with amidoxime, allowing dehydrating-cyclization to render compound 153, and then conducting deprotection.
In producing compound 153 from compound 152, a base may be added. As the base to be used, for example, triethylamine, pyridine, sodium hydride and the like can be recited. As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, and examples of such solvent include, but are not limited to, tetrahydrofuran, dimethoxyethane and N,N-dimethylformamide. The reaction temperature is room temperature or reflux temperature of the solvent.
Deprotection of compound 153 is conducted in accordance
with Production method 3. Production method 77
(Formula Removed)
Compound 156 can be produced by reacting compound 131 produced by Production method 66 and compound 155.
In the reaction between amidine 131 and compound 155, a
base is used as necessary. As the base, triethylamine,
diisopropylethylamine, potassium carbonate and the like are
recited. As the solvent, any solvents can be used insofar as
they are not concerned with the reaction, and preferred examples
of such solvent include, but are not limited to, methanol, ethanol,
pyridine, acetic acid, tetrahydrofuran, toluene, 1,4-dioxane,
N,N-dimethylformamide and the like. The reaction temperature
is usually from room temperature to reflux temperature of
solvent.
Production method 78
(Formula Removed)
Compound 157 can be produced by reacting imidate 115 produced
by Production method 57 or thioimidate 116 produced by Production method 58 with hydra zide in the absence of abase. As the reaction solvent, any solvents can be used insofar as they are not concerned with the reaction, andpreferred examples of such solvent include, but are not limited to, methanol, ethanol, propanol, butanol, tetrahydrofuran, toluene, 1,4-dioxane, dimethylformamide, pyridine and the like. The reaction temperature is usually from room temperature to reflux temperature of solvent. Production method 79
(Formula Removed)
Compound 158 can be produced by reacting imidate 115 produced
by Productionmethod 57 or thioimidate 116 producedby Production method 58 with hydrazide in accordance with the method described in Production method 57 for producing compound 114. Production method 80
(Formula Removed)
Compound 159 can be producedby reacting imidate 115 produced by Production method 57 or thioimidate 116 producedby Production method 58 with hydrazide in accordance with the method described in Production method 57 for producing compound 114.
The "salt" used herein refers to any pharmaceutically acceptable salts that form salts with compounds in accordance with the present invention and are, and preferably, but are not limited to, hydrohalic acid salts (for example, hydrofluoric acid salts, hydrochloric acid salts, hydrobromic acid salts, hydroiodic acid salts and the like), inorganic acid salts (for example, sulfuric acid salts, nitric acid salts, perchloric acid salts, phosphoric acid salts, carbonic acid salts, bicarbonic acid salts and the like), organic carboxylic acid salts (for example, acetic acid salts, maleic acid salts, tartaric acid salts, fumaric acid salts, citric acid salts and the like), organic sulfonic acid salts (for example, methanesulfonic acid salts, ethanesulfonic acid salts, benzenesulfonic acid salts, toluenesulfonic acid salts, camphorsulfonic acid salts and the like), amino acid salts (for example, aspartic acid salts, glutamic acid salts and the like), quaternary amine salts, alkaline metal salts (for example, sodium salts, potassium salts and the like), alkaline earth metal salts (magnesium salts, potassium salts and the like) and the like, and more preferably hydrochloric acid salts, sulfuric acid salts, methanesulfonic acid salts, acetic acid salts and the like.
The compounds represented by the above formulae (I) to (III)
or their salts or hydrates thereof in accordance with the present invention canbe formulated in conventionalmethods, andexamples of preferred dosage forms include tablet, powder, fine grain agent, granule, coating tablet, encapsulated formulation, syrup, troche, inhalant, suppository, injection, ointment, eye ointment agent, eye drop, nasal drop, ear drops, cataplasm, lotion and the like. In preparation, commonly used excipient, binder, disintegrator, lubricant, colorant, flavoring agent, as well as stabilizer, emulsifying agent, absorption promoter, surfactant, pH modifier, antiseptics, anti-oxidant and the like can be used as necessary, and preparation is achieved by means of conventional methods while blending components that are generally used as raw materials of pharmaceutical formulation. Examples of the above components include: (1) animal and vegetable oils such as soybean oil, beef tallow or synthesis glyceride; (2) hydrocarbons such as liquid paraffin, squalane or solid paraffin; (3) ester oils such as octyldodecyl myristate or isopropyl myristate; (4) higher alcohols such as ceto-stearyl alcohol or behenyl alcohol; (5) silicon resin; (6) silicon oil; (7) surfactants such as polyoxyethylene fatty acid esters, sorbitan fatty acid esters, glycerine fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene hardened castor oil or polyoxyethylene polyoxypropylene block copolymer; (8) water-soluble polymers such as hydroxyethyl cellulose, polyacrylic acid, carboxy vinyl polymer, polyethylene glycol, polyvinylpyrrolidone or methyl cellulose;
(9) lower alcohols such as ethanol or isopropanol; (10) polyols such as glycerin, propylene glycol, dipropylene glycol or sorbitol; (11) sugars such as glucose or sucrose; (12) inorganic powders such as silicic anhydride, aluminum silicate magnesium or aluminum silicate; and (13) purified water.
1) Examples of excipients include lactose, corn starch, saccharose, glucose, mannitol, sorbit, crystalline cellulose, silicon dioxide and the like; 2) examples of binders include polyvinyl alcohol, polyvinyl ether, methyl cellulose, ethyl cellulose, gum Arabic, gum tragacanth, gelatine, shellac, hydroxypropylcellulose, hydroxypropyl methylcellulose, polyvinylpyrrolidone, polypropylene glycol polyoxyethylene block polymer, meglumine, calcium citrate, dextrin, pectin and the like; 3) examples of disintegrators include starch, agar, gelatin powder, crystalline cellulose, calcium carbonate, sodium hydrogen carbonate, calcium citrate, dextrin, pectin, carboxymethylcellulose calcium and the like; 4) examples of lubricants include magnesium stearate, talc, polyethylene glycol, silica, consolidation vegetable oil and the like; 5) examples of colorants include those accepted to be added to pharmaceuticals; 6) examples of flavoring agents include cocoa powder, menthol, aroma powder, mentha oil, borneol, cassia bark and the like; and 7) examples of anti-oxidants include ascorbic acid, a-tocopherol and the like that are accepted to be added to pharmaceuticals.
1) As to oral formulations, after blending the compound
or its pharmaceutically acceptable salt according to the present invention and an excipient, as well as a binder, disintegrator, lubricant, colorant, flavoring agent and the like as necessary, the mixture is formed into powder, fine grain agent, granule, tablet, coating tablet, encapsulated formulation and the like. 2) In the cases of tablets and granules, it is allowable to appropriately coat with sugar, gelatin and other materials if necessary. 3) Liquid agents such as syrup, injection formulation or eye drop may produced by blending the compound according to the present invention or a pharmaceutically acceptable salt thereof with pH regulating agents, resolvents, tonicity agents, etc., optionally together with dissolution aids, stabilizers, bufferagents, suspending agents, antioxidants etc. and processing the resultant blends into preparations by the conventional methods. Such liquid agents may be lyophilized and injection may be subcutaneously or intramuscularly administered. Preferred examples of suspending agents include methyl cellulose, polysolvate 80, hydroxymethyl cellulose, gum Arabic, tragacanth powder, carboxymethylcellulose sodium, polyoxyethylene sorbitanmonolaurate and the like; preferred examples of dissolution aids include polyoxyethylene hardened castor oil, polysolvate 80, nicotinamide, polyoxyethylene sorbitanmonolaurate and the like; preferred examples of stabilizers include sodium sulfite, sodium metasulfite, ether and the like; and preferred examples of preservatives include methyl paraoxybenzoate, ethyl paraoxybenzoate, sorbic acid,
phenol, cresol, chlorocresol and the like. 4) In the cases of External preparations, may be produced by the conventional methods without limitation. As the bases, use can be made of variousmaterials commonlyusedindrugs, quasidrugs, cosmetics, etc. Particular examples of the base materials include animal and vegetable oils, mineral oils, ester oils, waxes, higher alcohols, fatty acids, silicone oils, surfactants, phospholipids, alcohols, polyhydric alcohols, water-soluble polymers, clay minerals and purified water. If needed, it is possible to further add pH regulating agents, antioxidants, chelating agents, antiseptics, fungicides, coloring agents, perfumes, etc. If necessary, it is also possible to further add other ingredients capable of inducing differentiation, blood flow accelerators, bactericides, antiinflammatory agents, cell activators, vitamins, amino acids, humectants, keratolytic agents, etc.
The dosage amount of the pharmaceutical according to the present invention differs depending on the severity of symptom, age, sex, body weight, dosage form, type of the salt, sensitivity to drug, particular type of the disease, and the like, and generally, these compounds are administered to an adult in a dose of about 30 ug to 1,000 mg, preferably from 100 ug to 500 mg and Stille preferably from 100 ug to 100 mg, per day once or several times a day. In the case of injection, generally about 1 µg/kg to 3,000 µg/kg, and preferably about 3 µg/kg to 1,000 µg/kg is administered.
In accordance with the present invention, novel indazole compounds are provided. The compounds (I) to (III) or their salts according to the present invent ion have excel lent selective inhibiting effect on c-Jun amino terminal kinase (JNK), especially on JNK 3. Therefore, the compounds (I) to (III) or their salts and pharmaceutical compositions containing the same are useful as therapeutic agents or preventive agents for immunological diseases, inflammatory disease, metabolic diseases and/or neurodegenerative diseases, and particularly useful as therapeutic agents or preventive agents for acute neurodegenerative diseases (for example, cerebrovascular disorder acute stage, headinjury, spinal cordinjury, neuropathy due to low oxygen, neuropathy low blood sugar and the like) , chronic neurodegenerative diseases (for example, Alzheimer's disease, Parkinson's disease, Huntington's chorea, amyotrophic lateral sclerosis, multiple sclerosis, spinocerebellar degeneration and the like), epilepsy, hepatic encephalopathy, peripheral neuropathy, Parkinson syndrome, exanthematous paralysis, pain, neuralgia, infectious encephalomyelitis, cerebrovascular dementia, dementia or neurosis due tomeningitis and the like.
Examples
Any Production examples, Examples and Test examples provided below are merely illustrative, and compounds according to the present invention are not restricted by the following
concrete examples. Those skilled in the art can conduct the present invention to the utmost while making various modifications within the scope of claims associated with the present description as well as the Examples provided below, and all suchmodif ications are involved in the boundary of the present description. Production example 1 (3-Fluorophenyl)-(3-fluoropyridin-2-yl)-methanol
Under nitrogen atmosphere, a solution of 9.75g of diazabicyclo [2.2.0] octane in 150 mL of dehydrated diethyl ether was cooled to -40°C, added with 56 mL of 1.56 M n-butyllithium in hexane, and stirred at -20°C for 1 hour. The solution was cooled to -60°C, added dropwise with 6. 9 mL of 3-fluoropyridine, stirred at -60°C for 1 hour, and added with 9.2 mL of 3-fluorobenzaldehyde. After stirring for 1 hour, aqueous ammonium chloride and ethyl acetate were successively added, and the mixture was recovered to room temperature. The organic layer was extracted, washed with water, and then dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting crude product was purified and separated by silica gel column chromatography (ethyl acetate: hexane=l:4) , to give 12.6 g of the title compound as colorless needle crystals. 1H-NMR (400 MHz, CDC13) δ 5.26 (1H, d, J = 6.8 Hz), 5.98 (1H, d, J = 6.8 Hz), 6.95 (1H, dt, J = 2.4, 8.4 Hz), 7.10 (1H, bd, J = 9.8 Hz), 7.20 (1H, bd, J = 8.4 Hz), 7.29 (1H, dt, J = 5.5, 8.4 Hz) , 7.30 (1H, dd, J = 4.9, 8.8 Hz) , 8.3 9 (1H, dt, J = 1.2,

8.8 Hz), 8.42 (1H, d, J = 1.2, 4.9 Hz).
Production example 2
(3-Fluorophenyl)-(3-fluoropyridin-2-yl)-methanone
A mixed solution of 12.6g of (3-fluorophenyl)-(3-fluoropyridin-2-yl)-methanol obtained by Production example 1 in 30 mL dichloromethane and 30 mL toluene was added with 10 . 0 g of activated manganese dioxide, and heated under reflux for 8 hours. After completion of the reaction, manganese dioxide was filtered off through Celite, and the solvent was evaporated, to give 12.1 g of the title compound as a pale yellow oil.
1H-NMR (4 00 MHz, CDCl3) δ 7.32 (1H, ddt, J = 1.4, 2.8, 7.9 Hz), 7.47 (1H, dt, J = 5.3, 7.9 Hz), 7.54 (1H, dd, J=4.5, 9.0 Hz), 7.61 (1H, dt, J = 1.4, 9.0 Hz), 7.66 (1H, ddd, J = 1.4, 2.8, 9.2 Hz), 7.72 (1H, dt, J=1.4, 7.9 Hz), 8.54 (1H, dt, J=1.4, 4.5 Hz). Example 3 3-(3-Fluorophenyl)-2H-pyrazolo[4,3-b]pyridine
1.5 g of (3-fluorophenyl)-(3-fluoropyridin-2-yl)-methanone was dissolved in 5 mL of methanol, added with 1.0 mL of hydrazine monohydrate, and heated at 80°C for 4 hours. The reaction solution was added with water, and extracted with ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off, and the residue was purified and
separated by silica gel column chromatography (ethyl acetate:n-hexane=l:4), to afford 240 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 7.21 (1H, dt, J = 2.4, 8.0 Hz) ,7.44
(1H, dd, J = 4.1, 8.6 Hz), 7.55 (1H, dt, J = 6.0, 8.0 Hz), 8.07
(1H, dd, J = 1.5, 8.6 Hz), 8.30 (1H, dd, J = 2.4, 10.9 Hz), 8.35
(1H, d, J= 8.0Hz), 8.64 (1H, dd, J=1.5, 4.1Hz), 13.47-13.53
(1H, bs). Production example 4
(3-Fluoro-l-oxypyridin-2-yl)-(3-fluorophenyl)-methanone 3.0 g of
(3-fluorophenyl)-(3-fluoropyridin-2-yl)-methanone obtained by Production example 2 was dissolved in 30 mL of chloroform, added with 3. 6 g of 3-chloro perbenzoic acid under ice-cooling, and the reaction solution was heated under reflux for 6 hours. After completion of the reaction, aqueous sodium hydrogen carbonate was added, extracted with ethyl acetate, and the organic layer was washed with water and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting residue was purified and separatedby silica gel column chromatography (ethyl acetate: methanol=19:1), to afford 2.1 g of the title compound as colorless needle crystals. 1H-NMR (400 MHz, CDCl3) δ 7.21 (1H, ddd, J = 0.7, 6.7, 8.9 Hz) , 7.35 (1H, ddt, J = 1.2, 2.6, 8.0 Hz), 7.40 (1H, dt, J = 6.7, 8.9Hz), 7.49 (1H, dt, J=5.4, 8.0Hz), 7.58 (1H, ddd, J=1.2, 2.6, 8.9 Hz), 7.62 (1H, dt, J = 1.2, 8.0 Hz), 8.14 (1H, dt, J
= 0.7, 6.7 Hz) .
Production example 5
5-Fluoro-6-(3-fluorobenzoyl)-pyridine-2-carbonitrile
To 20 mL of a solution of 2.1 g of (3-fluoro-l-oxypyridin-2-yl)-(3-fluorophenyl)-methanone obtained by Production example 4 in acetonitrile were added 6.0 mL of trimethylsilylcyanide and 1.7 mL of dimethylcarbamoyl chloride, and heated under reflux for 8 hours . After completion of the reaction was added aqueous sodium hydrogen carbonate and extracted with ethyl acetate, and the organic layer was washed with water, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:9), to afford 1.0 g of a crude product of the title compound as a colorless oil. Example 6
3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-5-carbonitrile
From 1.0 g of crude 5-fluoro-6-(3-fluorobenzoyl)-pyridine-2-carbonitrile, 280 mg of the title compound was obtained as a pale yellow powder in accordance with Production example 3.
1H-NMR (4 00 MHz, DMSO-D6) δ 7.28 (1H, dt, J = 2.4, 8.0 Hz), 7. 61 (1H, dt, J = 6.4, 8.0 Hz) , 7.98 (1H, dt, J = 8.5 Hz) , 8.16 (1H, dd, J = 2.4, 10.6 Hz), 8.28 (1H, d, J = 8.0 Hz), 8.32 (1H, d, J = 8.5 Hz), 13.95-14.20 (1H, bs).
Example 7
3- (3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid
140 mg of 3-(3-fluorophenyl)-lH-pyrazolo[4,3-b]-pyridine-5-carbonitrile was added to a mixed solution of 1 mL water, 1 mL concentrated sulfuric acid and 1 mL glacial acetic acid, and heated under 100°C for 3 hours. The reaction solution was added with 25 mL of ice-cooled water, neutralized with sodium hydrogen carbonate, and theprecipitated crystals were collected by filtration. The crystals collectedby filtration were washed with cold water, and dried under reduced pressure, to give 150 mg of a crude product of the title compound as a colorless powder. 1H-NMR (4 00 MHz, DMSO-D6) δ 7.17 (1H, dt, J = 2.8, 8.1 Hz), 7.52 (1H, dt, J = 6.8, 8.1 Hz), 7.8 9 (1H, d, J = 9.0 Hz), 7.95 (1H, d, J = 9.0 Hz) , 8.38 (1H, d, J = 8.1 Hz) , 8.4 0 (1H, bd, J = 11.3 Hz) .
Production example 8 (3-Fluoropyridin-2-yl)-(naphthalen-2-yl)-methanol
In the manner as described in Production example 1 while using 5.2 mL of 3-fluoropyridine and 10.2 g of 2-naphthaldehyde as starting materials, 3.8 g of the title compound was obtained as pale yellow needle crystals.
1H-NMR (400 MHz, CDCl3) δ 5.34 (1H, d, J = 6.8 Hz), 6.17 (1H, d, J = 6.8 Hz), 7.29 (1H, dd, J = 4.9, 8.6 Hz), 7.36 (1H, dt, J = 1.0, 8.6 Hz), 7.42-7.50 (3H, m), 7.77-7.84 (1H, m) , 7.79 (1H, d, J = 8.5 Hz), 7.83 (1H, d, J = 8.5 Hz), 7.88 (1H, s) ,
8.45 (1H, dt, J = 1.0, 4.9 Hz) .
Production example 9
(3-Fluoropyridin-2-yl)-(naphthalen-2-yl)-methanone
From 3.8 g of (3-fluoropyridine2-yl)-(naphthalene-2-yl)-methanol, 3.4 g of the title compound was obtained as colorless needle crystals in accordance with Production example 2.
1H-NMR (400 MHz, CDCl3) δ 7.54 (1H, dt, J = 1.3, 7.4 Hz), 7.55 (1H, dd, J = 4.6, 8.8 Hz) , 7.62 (1H, dt, J = 1.3, 8.8 Hz) , 7. 64 (1H, dt, J = 1.3, 7.4 Hz) , 7.90 (1H, bd, J = 7.4 Hz) , 7.91 (1H, bd, J = 7.4 Hz), 7.94 (1H, d, J = 8.6 Hz), 8.07 (1H, dd, J = 1.5, 8.6 Hz), 8.36 (1H, d, J= 1.5 Hz), 8.58 (1H, dt, J = 1.3, 4.6 Hz) . Example 10 3-(Naphthalen-2-yl)-IH-pyrazolo[4,3-b]pyridine
From 1.2 g of (3-fluoropyridin-2-yl)-(naphthalen-2-yl)-methanone, 460 mg of the title compound was obtained as colorless needle crystals in the same manner as described in Production example 3. 1H-NMR (400 MHz, DMSO-D6) δ 7.44 (1H, dd, J = 4.1, 8.8 Hz) , 7.53 (1H, t, J = 8.5 Hz), 7.55 (1H, t, J = 8.5 Hz), 7.84 (1H, bd, J = 8.8 Hz), 8.03 (2H, d, J = 8.5 Hz), 8.09 (1H, dd, J = 0.6, 8.8 Hz) , 8.57 (1H, dd, J = 1.8, 8.8 Hz) , 8.69 (1H, dd, J = 0.6, 4.1 Hz), 9.17 (1H, d, J = 1.8 Hz). Production example 11 (3-Fluoro-l-oxypyridin-2-yl)-naphthalen-2-yl-methanone
From 1.5 g of (3-fluoropyridin-2-yl)-(naphthalen-2-yl)-methanone obtained by Production example 9, 0. 8 g of the title compound was obtained as colorless needle crystals in accordance with Production example 4.
1H-NMR (400 MHz, CDCl3) δ 7.24 (1H, ddd, J = 0.8, 6.5, 8.8 Hz), 7.41 (1H, dt, J = 6.5, 8.8 Hz) , 7.55 (1H, dt, J = 1.4, 7.4 Hz) , 7.63 (1H, dt, J = 1.4, 7.4 Hz) , 7.8 9 (1H, bd, J = 7.4 Hz) , 7.91 (1H, bd, J = 7.4 Hz), 7.95 (1H, d, J = 8.8 Hz), 8.01 (1H, dd, J = 1.5, 8.8 Hz), 8.18 (1H, dt, J = 0.8, 6.5 Hz), 8.27 (1H, d, J = 1.5 Hz) . Production example 12 5-Fluoro-6-(naphthalene-2-carbonyl)-pyridine-2-carbonitrile
From 780 mg of (3-fluoro-l-oxypyridin-2-yl)-naphthalen-2-yl-methanone obtained by Production example 11, 550 mg of a crude product of the title compound was obtained as a pale yellow oil in the same manner as described in Production example 5. 1H-NMR (400MHz, CDCl3) δ7.49 (1H, dd, J=8.1, 9.5Hz), 7.52-7.57 (2H, m) , 7.57 (1H, dd, J = 2.0, 8.8 Hz) , 7.7 7 (1H, dd, J = 3.3, 8.1 Hz),7.82-7.86 (1H, m),7.85 (1H, d, J = 8.8 Hz),7.89-7.93 (1H, m),8.13 (1H, d, J = 2.0 Hz). Production example 13 3-(Naphthalen-2-yl)-1-trityl-lH-pyrazolo[4,3-b]pyridine
From 44 0 mg of 3-(naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine, 800 mg of the
title compound was obtained as a colorless powder in accordance with Production example 22.
1H-NMR (400 MHz, CDCl3) δ 6.66 (1H, dd, J = 1.1, 8.8 Hz), 6.95 (1H, dd, J = 4.2, 8.8 Hz), 7.25-7.34 (15H, m), 7.43-7.51 (2H, m) , 7.83 (1H, dd, J = 2.3, 7.9 Hz), 7.88 (1H, d, J = 8.5 Hz), 8.00 (1H, dd, J=2.3, 7.9 Hz), 8.42 (1H, dd, J=2.0, 8.5 Hz), 8.62 (1H, dd, J = 1.1, 4.2 Hz), 9.18 (1H, d, J = 2.0 Hz). Production example 14
3-(Naphthalen-2-yl)-l-trityl-lH-pyrazolo[4,3-b]pyridine-4-oxide
From 8 00 mg of 3-(naphthalen-2-yl)-l-trityl-2H-pyrazolo[4,3-b]pyridine, 500 mg of the title compound was obtained as colorless crystals in the same manner as described in Production example 4.
1H-NMR (400 MHz, DMSO-D6) δ 6.36 (1H, d, J =8.6 Hz), 7.10 (1H, dd, J = 5.8, 8.6 Hz) , 7.24 (6H, bd, J = 6.9 Hz) , 7.30-7.4 0 (9H,m), 7.50-7.55 (2H,m), 7.90 (1H, d, J =8.6 Hz), 7.90-7.94 (2H,m), 7.99 (1H, dd, J = 1.9, 8.6 Hz), 8.13 (1H, d, J = 5.8 Hz) , 8.60 (1H, d, J = 1.9 Hz). Production example 15
3- (Naphthalen-2-yl) -1-trityl-lH-pyrazolo [4, 3-b]pyridine-5-carbonitrile
From 480 mg of 3-naphthalen-2-yl-l-trityl-lH-pyrazolo[4,3-b]pyridine-4-oxide, 380 mg of the title compound was obtained as pale yellow crystals in the same manner as described in Production example 5.
1H-NMR (4 00 MHz, DMSO-D6) δ 6.89 (1H, d, J= 9.1Hz), 7.23-7.28 (6H,m), 7.33-7.42 (9H,m), 7.54-7.59 (2H,m), 7.77 (1H, d, J = 9.1Hz), 7.92-7.97 (lH,m), 8.02-8.07 (lH,m), 8.04 (1H, d, J= 8.7 Hz), 8.25 (1H, dd, J = 1.8, 8.7 Hz), 8.97 (1H, d, J = 1.8 Hz) .
Example 16
3-(Naphthalen-2-yl)-Itf-pyrazolo[4,3-b]pyridine-5-carbonitrile
To 10 mL of a solution containing 360 mg of 3-naphthalen-2-yl-l-trityl-lH-pyrazolo[4,3-b]pyridine-5-carbonitrile obtained by Production example 15 in dichloromethane, 3 mL of trif luoroacetic acid was added at room temperature and stirred for 2 hours. The reaction solution was added with aqueous sodium hydrogen carbonate, extracted with ethyl acetate, washed with water, and dried over magnesium sulfate. After evaporating, the residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=3:1) , to afford 180 mg of the title compound as a colorless powder.
1H-NMR (400MHz, DMSO-D6) δ 7.53-7.60 (2H, m), 7.94-7.99 (1H, m) , 8.00 (1H, d, J = 8.6 Hz), 8.05-8.10 (1H, m), 8.08 (1H, d, J = 8.6 Hz), 8.33 (1H, d, J = 8.6 Hz), 8.51 (1H, dd, J = 1.3, 8.6 Hz), 9.02 (1H, d, J = 1.3 Hz), 14.02-14.13 (1H, bs).
The title compound was also synthesized in an alternative method as described below.
Using 550 mg of crude
5-fluoro-6-(naphthalene-2-carbonyl)-pyridine-2-carbonitrile
obtained by Production example 12 as a starting material, 3 mg
of the title compound was obtained in the method similar to
Production example 3.
Example 17
3-(Naphthalen-2-yl)-2H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid
From 180 mg of 3-(naphthalen-2-yl)-1H-pyrazolo [4 , 3-b] pyridine-5-carbonit rile obtained by Example 16, 150 mg of the title compound was obtained as a colorless powder in the same manner as described in Example 7. 1H-NMR (400 MHz, DMSO-D6) δ 7.51-7.59 (2H, m) , 7.95 (1H, bd, J = 7.9 Hz), 8.03 (1H, bd, J = 7.9 Hz), 8.06 (1H, d, J = 8.6 Hz), 8.12 (1H, d, J = 8.6 Hz), 8.20 (1H, d, J = 8.6 Hz), 8.63 (1H, dd, J=1.6, 8.6Hz), 9.18 (1H, d, J=1.6Hz), 13.12-13.27 (1H, bs), 13.81 (1H, s) . Production example 18 (3-Fluorophenyl)-(3-fluoropyridin-4-yl)-methanol
Under nitrogen atmosphere, a solution of 6.1 mL of diisopropylamine in 100 ml of dehydrated tetrahydrofuran was cooled to -70°C, added with 28 mL of 1.56 M n-butyllithium in hexane, and stirred at 0°C for 15 minutes. After cooling to -70°C, 3.4 mL of 3-fluoropyridine was added dropwise, stirred at the same temperature for 4 hours, and then added dropwise with 4 . 8 mL of 3-fluorobenzaldehyde. After stirring for 1 hour, aqueous ammonium chloride and ethyl acetate were successively
added, and the reaction solution was recovered to room temperature. The organic layer was extracted, washed with water, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting residue was recrystallized from diisopropyl ether, to afford 7.1 g of the title compound as colorless cubic crystals.
1H-NMR (400MHz, CDCl3) δ 2.70-3.10 (1H, bs), 6.13 (1H, s), 7.00 (1H, ddd, J = 0.9, 1.7, 8.3 Hz), 7.13 (1H, dt, J = 1.7, 9.2 Hz), 7.18 (1H, dt, J = 0.9, 8.3 Hz) , 7.32 (1H, dt, J = 5.8, 8.3 Hz) , 7.57 (1H, t, J = 5.5 Hz), 8.37 (1H, d, J = 1.4 Hz), 8.43 (1H, d, J = 5.5 Hz). Production example 19 (3-Fluorophenyl)-(3-fluoropyridin-4-yl)-methanone
From 3.5 g of (3-fluorophenyl)-(3-fluoropyridin-4-yl)-methanol, 3 . 5 g of the title compound was obtained as colorless needle crystals in accordance with Production example 2.
1H-NMR (400MHz, CDCl3) δ 7.37 (1H, bt, J =7.8 Hz), 7.43 (1H, t, J = 5.4 Hz), 7.50 (1H, dt, J = 5.4, 7.8 Hz) , 7.54-7.60 (2H, m), 8.62 (1H, dd, J = 1.1, 4.8 Hz), 8.66 (1H, d, J= 1.1 Hz). Production example 20 (3-fluoro-l-oxypyridin-4-yl)-(3-fluorophenyl)-methanone
From 1.8 g of (3-fluorophenyl)-(3-fluoropyridine-4-yl)-methanone, 1.65 g of the title compound was obtained as colorless needle crystals in accordance with Production example 4.
1H-NMR (400 MHz, CDCl3) δ 7.37 (1H, ddt, J = 1.2, 2.5, 8.1 Hz), 7.48-7,59 (4H, m), 8.13 (1H, ddd, J = 1.0, 1.5, 6.8 Hz), 8.20
(1H, dt, J = 1.5, 5.3 Hz) . Example 21
3-(3-Fluorophenyl)-Itf-pyrazolo[3,4-c]pyridine From 2.0 g of
(3-fluorophenyl)-(3-fluoropyridin-4-yl)-methanone obtained by Production example 19, 490mg of the title compound was obtained as colorless powder in accordance with Production example 3. 2H-NMR (400 MHz, DMSO-D6) δ 7.26 (1H, dt, J =2.6, 8.2 Hz), 7.58
(1H, dt, J = 6.6, 8.2 Hz), 7.78 (1H, dd, J = 2.6, 10.4 Hz), 7.89
(1H, d, J = 8.2 Hz), 8.10 (1H, dd, J = 1.2, 5.9 Hz), 8.31 (1H, d, J = 5.9 Hz), 9.10 (1H, d, J = 1.2 Hz). Production example 22 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[3,4-c]pyridine
To a solution of 2.43 g of 3-(3-fluorophenyl)-1H-pyrazolo[3,4-c]pyridine produced in Production example 21 in 10 mL of N,N-dimethylf ormamide was added 80 mg of ice-cooled 60% sodium hydride (oily) . After stirring 30 minutes, 500 mg of chlorotriphenylmethane was added and stirred at room temperature for 1 hour. The reaction solution was added with water, extracted with ethyl acetate, dried over anhydrous sodium sulfate, and the solvent was evaporated. The residue was added with diisopropyl ether and filtered, to afford 560 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 7.20-7.24 (6H, m), 7.26 (1H, dt, J
= 2.5, 8.1 Hz), 7.30-7.40 (9H, m), 7.55 (1H, dt, J = 6.4, 8.1
Hz), 7.64 (1H, ddd, J = 1.4, 2.5, 10.0 Hz), 7.74 (1H, d, J =
1.4 Hz), 7.78 (1H, dt, J = 1.4, 8.1 Hz), 8.13 (1H, dd, J = 1.4,
5.7 Hz), 8.24 (1H, d, J = 5.7 Hz).
Production example 23
3-(3-Fluorophenyl)-l-trityl-lH-pyrazolo[3,4,-c]pyridine-6-
oxide
From 300 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[3,4-c]pyridine, 285 mg of the title compound was obtained as a colorless powder in the same manner as described in Production example 4. 1H-NMR (400 MHz, CDCl3) δ 7.01 (1H, d, J = 1.4 Hz), 7.18-7.24 (6H, m) , 7.28 (1H, dt, J=2.5, 8.0Hz), 7.33-7.42 (9H, m) , 7.55 (1H, dt, J = 6.0, 8.0 Hz), 7.61 (1H, ddd, J = 1.4, 2.5, 10.1 Hz), 7.72 (1H, dt, J=1.4, 8.0Hz), 7.91 (1H, dd, J=1.4, 7.1 Hz), 8.14 (1H, d, J = 7.1 Hz). Production example 24
3-(3-Fluorophenyl)-l-trityl-2H-pyrazolo[3,4-c]pyridine-7-carbonitrile
From 200 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [3,4, -c] pyridine-6-oxide, 150 mg of the title compound was obtained as a colorless powder in accordance with Production example 5.
1H-NMR (400 MHz, CDCl3) δ 7.15-7.21 (6H, m) , 7.28-7.39 (10H, m) , 7.57 (1H, dt, J = 6.4, 8.0 Hz) , 7.59 (1H, ddd, J = 1.3, 2.5, 10.7 Hz), 7.72 (1H, dt, J = 1.3, 8.0 Hz), 8.52 (1H, d, J = 5.7

Hz) , 8.60 (1H, d, J = 5.7 Hz).
Production example 25
(3-Fluoropyridin-4-yl)-(naphthalen-2-yl)-methanol
Using 5.2 mL of 3-fluoropyridine and 10.2 g of 2-naphthaldehyde as starting materials, 17.0 g of the title compound was obtained as a colorless powder in the same manner as described in Production example 18.
1H-NMR (400MHz, CDCl3) δ 2.89 (1H, s), 6.27 (1H, s), 7.44-7.51 (3H, m) , 7.64 (1H, t, J = 5.7 Hz) , 7.80-7.85 (3H, m) , 7.87 (1H, bs), 8.35 (1H, d, J = 2.0 Hz), 8.42 (1H, dt, J = 1.0, 4.9 Hz). Production example 26 (3-Fluoropyridin-4-yl)-(naphthalen-2-yl)-methanone
From 17.0 g of (3-fluoropyridin-4-yl)-(naphthalen-2-yl)-methanol, 15.1 g of the title compound was obtained as colorless needle crystals in accordance with Production example 2.
1H-NMR (400 MHz, CDCl3) δ 7.49 (1H, dd, J = 4.6, 5.5 Hz), 7.58 (1H, dt, J = 1.3, 7.4 Hz) , 7.66 (1H, dt, J = 1.3, 7.4 Hz) , 7.93 (2H, bd, J = 7.4 Hz), 7.97 (1H, d, J = 8.9 Hz), 8.01 (1H, dd, J = 1.5, 8.9 Hz), 8.22 (1H, bd, J = 1.5 Hz), 8.64 (1H, dd, J = 1.3, 4.6 Hz), 8.68 (1H, d, J = 1.3 Hz). Production example 27 (3-Fluoro-l-oxypyridin-4-yl)-(naphthalen-2-yl)-methanone
From 1.5 g of (3-fluoropyridin-4-yl)-naphthalen-2-yl-methanone, 1. 3 gof the title compound was obtained as a colorless powder in accordance
with Production example 4.
1H-NMR (400 MHz, CDC13) δ 7.60 (1H, dd, J = 6.8, 7.8 Hz), 7.61
(1H, dt, J = 1.5, 7.5 Hz) , 7.68 (1H, dt, J = 1.5, 7.5 Hz) , 7.93
(1H, bd, J = 7.5 Hz), 7.95 (1H, bd, J = 7.5 Hz), 7.97 (1H, d,
J = 8.8 Hz), 7.98 (1H, d, J = 8.8 Hz), 8.17 (1H, ddd, J = 0.6,
1.8, 6.8 Hz), 8.25 (1H, dd, J = 1.8, 5.0 Hz), 8.26 (1H, s).
Production example 28
(2-Chloro-5-fluoropyridin-4-yl)-(naphthalen-2-yl)-methanone
A solution containing 1.4 g of (3-fluoro-l-oxypyridin-4-yl)-(naphthalen-2-yl)-methanone obtained by Production example 27 in 10 mL of phosphorus oxychloride was heated under nitrogen atmosphere at 80°C for 1.5 hours. The reaction solution was allowed to cool to room temperature, and the excess phosphorus oxychloride was distilled off under reducedpressure . The resulting residue was then added with aqueous sodium hydrogen carbonate, extracted with ethyl acetate, and the organic layer was washed with water and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=19:1), to afford 680 mg of the title compound as colorless needle crystals and 550 mg of
(2-chloro-3-fluoropyridin-4-yl)-naphthalen-2-yl-methanone described in Production example 29 described below as colorless needle crystals. 1H-NMR (400 MHz, CDCl3) δ 7.50 (1H, d, J = 4.6 Hz), 7.60 (1H,
t, J= 7.5 Hz), 7.68 (1H, t, J= 7.5 Hz), 7.93 (1H, d, J = 7.5 Hz),7.95 (1H, d, J = 7.5 Hz),7.98 (1H, d, J = 9.1 Hz),7.99 (1H, d, J = 9.1 Hz),8.21 (1H, bs),8.45 (1H, s). Production example 2 9
(2-Chloro-3-fluoropyridin-4-yl)-(naphthalen-2-yl)-methanone 1H-NMR (400 MHz, CDCl3) δ 7.41 (1H, t, J = 4.6 Hz), 7.60 (1H, t, J = 7.5 Hz), 7.68 (1H, t, J = 7.5 Hz), 7.93 (1H, d, J = 7.5 Hz),7.95 (1H, d, J = 7.5 Hz),7.98 (1H, d, J = 9.1 Hz),7.99 (1H, d, J = 9.1 Hz),8.21 (1H, bs),8.41 (1H, d, J = 4.6 Hz). Production example 30 2-Benzyl-5-phenyl-2H-pyrazol-3-ylamine
2.9 g of α-cyanoacetophenone and 3.9 g of benzyl hydrazine dihydrochloride were suspended in 50 mL of ethanol. The reaction solution was added with 6.0 ml of triethylamine at room temperature, and heated under reflux for 4 hours. The solvent was evaporated, and the residue was added with water, extracted with ethyl acetate, and dried over anhydrous sodium sulfate. After distilling off the solvent, the residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:4), to afford 4.1 g of the title compound as a yellow needle powder.
1H-NMR (4 00 MHz, CDCl3) δ 3.40 (2H, bs) , 5.28 (2H, s) , 5.92 (1H, s), 7.22 (2H, d, J=8.0Hz), 7.28 (2H, t, J=8.0Hz), 7.32-7.37 (2H, m), 7.38 (2H, t, J = 8.0 Hz), 7.77 (2H, d, J = 8.0 Hz). Production example 31 l-Benzyl-3-phenyl-lH-pyrazolo[3,4-b]pyridine-5-carbonitrile
1.65 g of 2-cyano-3,3-dimethoxy-l-propenolate sodium was suspended in 30 mL of methanol, and the reaction solution was added successively with 2.5 g of
2-benzyl-5-phenyl-2H-pyrazole-3-ylamine obtained by Production example 30 and 1.5 mL of concentrated hydrochloric acid at room temperature, and heated under reflux for 3 hours. The reaction solution was added with aqueous sodium hydrogen carbonate, extractedwithethyl acetate, and driedover anhydrous sodium sulfate. After distilling off the solvent, the residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:9), to afford 0.8 g of the title compound as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 5.78 (2H, s), 7.28-7.37 (3H, m) , 7.43 (2H, bd, J = 7.2 Hz), 7.47 (2H, t, J = 7.2 Hz),7.54 (2H, t, J = 7.2 Hz) , 7.91 (2H, bd, J = 7.2 Hz) , 8. 66 (1H, d, J = 1.9 Hz) , 8.78 (1H, d, J = 1.9 Hz). Production example 32
1-Benzy1-3-phenyl-1H-pyrazolo [3, 4-b] pyridine-5-carboxylic acid
Using 600 mg of l-benzyl-3-phenyl-lH-pyrazolo[3,4-b]pyridine-5-carbonitrile as a starting material, 500 mg of the title compound was obtained as a colorless powder in the same manner as described in Example 7. 1H-NMR (400 MHz, DMSO-D6) δ 5.78 (2H, s) , 7.24-7.30 (5H, m) , 7.47 (1H, t, J = 8.6 Hz), 7.56 (2H, t, J = 8.6 Hz), 8.01 (2H, d, J = 8.6 Hz), 8.97 (1H, d, J = 2.0 Hz), 9.12 (1H, d, J = 2.0
Hz) .
Production example 33
l-Benzyl-3-phenyl-lH-pyrazolo[3, 4-b]pyridine-5-carboxylic
acid methyl ester
100 mg of l-benzyl-3-phenyl-2H-pyrazolo[3,4-b]pyridine-5-carboxylic acid obtained by Example 32 was dissolved in 5 mL of N,N-dimethylf ormamide, added with 60 mg of potassium carbonate and 30 ul of methyl iodide, and stirred all day and night at room temperature. The residue was added with water, extracted with ethyl acetate, and dried over anhydrous sodium sulfate. After distilling off the solvent, the reaction was crystallized from diisopropyl ether, to afford 100 mg of the title compound as a pale brown powder. 1H-NMR (4 00 MHz, DMSO-D6) δ 4.00 (3H, s) , 5.8 0 (2H, s) , 7.25-7.34 (3H, m) , 7.41-7.4 8 (3H, m) , 7.53 (2H, t, J = 7.6 Hz) , 7.98 (2H, d, J = 7.6 Hz), 9.01 (1H, d, J = 1.8 Hz), 9.22 (1H, d, J = 1.8 Hz) .
Example 34 3-Phenyl-2H-pyrazolo [3, 4-b]pyridine-5-carboxylic acid
100 mg of l-benzyl-3-phenyl-2H-pyrazolo[3,4-b]pyridine-5-carboxylic acid obtained by Production example 33 was dissolved in a mixed solvent of 2 mL sulfuric acid/ 4 mL acetic acid/ 2 mL water, and added with 300 mg of chromic acid under ice-cooling. The reaction solution was heated at 70 °C for 1 hour, allowed to cool to room temperature, added with sodium hydrogen carbonate, extracted with ethyl
acetate, and dried over anhydrous sodium sulfate. After
distilling off the solvent, the residue was added with
diisopropyl ether, followed by filtration, to afford 60 mg of
the title compound as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 7.46 (1H, t, J =7.6 Hz), 7.56 (2H,
t, J = 7.6 Hz), 8.02 (2H, d, J = 7.6 Hz), 8.94 (1H, d, J = 2.0
Hz), 9.06 (1H, d, J = 2.0Hz), 13.20-13.35 (1H, bs), 14.18 (1H,
s) -
Production example 35
3-(3-Fluorophenyl)-1-trityl-1H-pyrazolo[4,3-b]pyridine
From 220 mg of 3-(3-fluorophenyl)-1H-pyrazolo[4,3-b]pyridine obtained by Production example 3, 4 60 mg of the title c.ompound was obtained as a colorless powder in accordance with Production example 22.
1H-NMR (400 MHz, CDCl3) δ 6.64 (1H, dd, J = 1.8, 8.7 Hz), 6.93 (1H, dd, J=4.6, 8.7Hz), 7.02 (1H, dt, J = 2.5, 8.0Hz), 7.21-7.26 (6H, m) , 7.2 7-7.33 (9H, m) , 7.4 0 (1H, dt, J = 6.4, 8.0 Hz) , 8.19 (1H, dd, J = 2.5, 10.3Hz), 8.31 (1H, d, J=8.0Hz), 8.57 (1H, dd, J = 1.8, 4.6 Hz). Production example 36
3-(3-Fluorophenyl)-1-trityl-2H-pyrazolo[4,3-b]pyridine-4-oxide
From 4 80 mg of 3-(3-fluorophenyl)-1-trityl-2H-pyrazolo[4,3-b]pyridine, 250 mg of the title compound was obtained as a colorless powder in the same manner as described in Production example 4.
1H-NMR (400 MHz, CDCl3) δ 6.36 (1H, d, J = 8.8 Hz), 7.09 (1H,
dd, J = 6.2, 8.8 Hz), 7.17-7.24 (6H, m) , 7.29-7.39 (10H, m),
7.42 (1H, dt, J = 6.2, 8.2 Hz), 7.7 9 (1H, d, J = 8.2 Hz), 7.91
(1H, bd, J = 11.3 Hz), 8.13 (1H, d, J = 6.2 Hz).
Production example 37
3-(3-Fluorophenyl)-5-chloro-l-trityl-lH-
pyrazolo[4,3-b]pyridine
To 100 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[4,3-b]pyridine-4-oxide in toluene was acted phosphorus oxychloride in toluene in the same manner as described in Production example 28, to afford 30 mg of
3-(3-fluorophenyl)-5-chloro-lH-pyrazolo[4,3-b]pyridine described in Example 38 discribed below as a colorless powder, as well as 60 mg of a crude product of the title compound as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 6.54 (1H, d, J = 8.9 Hz), 6.92 (1H, d, J =8.9 Hz), 7.03 (1H, dt, J = 2.5, 8.1Hz), 7.17-7.23 (6H, m) , 7.24-7.35 (9H, m) , 7.4 0 (1H, dt, J = 6.0, 8.1 Hz) , 8.10 (1H, ddd, J = 1.9, 2.5, 10.7 Hz), 8.25 (1H, bd, J = 8.1 Hz). Example 38
3-(3-Fluorophenyl)-5-chloro-2H-pyrazolo[4,3-b]pyridine 1H-NMR (400 MHz, CDCl3) δ 7.11 (1H, ddt, J = 1.2, 2.8, 8.0 Hz) , 7.37 (1H, d, J = 8.7 Hz), 7.4 8 (1H, dt, J = 6.0, 8.0 Hz) , 7.82 (1H, d, J = 8.7 Hz) , 8.20 (1H, ddd, J = 1.5, 2.8, 10.4 Hz), 8.31 (1H, bd, J = 8.0 Hz), 10.20-10.40 (1H, bs).
Example 39
1-[3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridin-5-
yl]ethanone
50 mg of crude 3-(3-fluorophenyl)-5-chloro-l-trityl-lH-pyrazolo[4,3-b]pyridine obtained by Production example 37 and 35 µl of tributyl (1-ethoxyvinyl) tin was dissolved in 2 mL of N,N-dimethylformamide, added with 10 mg of
tetrakis(triphenylphosphine)palladium(0) , and heated at 100°C for 15 minutes. After completion of the reaction, the reaction solution was added with water and extracted with ethyl acetate. The organic layer was washed with water, dried over magnesium sulfate, and the solvent was evaporated. The residue was dissolved in a mixed solvent of 10 mL THF-1 mL water, added with 0. 5 mL of 5N hydrochloric acid and heated at 90°C for 10 minutes. After completion of the reaction, the reaction solution was added with aqueous sodium hydrogen carbonate and extracted with ethyl acetate. The organic layer was washed with water, dried over magnesium sulfate, and the solvent was evaporated. The residue was dissolved in 3 mL of dichloromethane, added with 0.5 mL of trifluoroacetic acid and stirred at room temperature for 5 minutes. The reaction solution was added with aqueous sodium hydrogen carbonate and extracted with ethyl acetate. The organic layer was washed with water, dried over magnesium sulfate, and the solvent was evaporated. The resulting residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=3:7), to afford 7 mg of the title compound
as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 2.78 (3H, s), 7.27 (1H, dt, J = 2.6,
7.9 Hz), 7.61 (1H, dt, J = 6.3, 7.9 Hz), 8.04 (1H, d, J = 8.9
Hz) , 8.20 (1H, d, J = 8.9 Hz) , 8.33 (1H, ddd, J = 1.3, 2.6, 10.4
Hz), 8.40 (1H, bd, J = 7.9 Hz), 13.82-13.96 (1H, bs).
Production example 4 0
[3-(3-Fluorophenyl)-l-trityl-2H-pyrazolo[4,3-b]pyridin-5-
yl] acetate
50 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [4 , 3-b] pyridine-4-oxide obtained by Production example 36 was suspended in 2 mL of acetic anhydride, and the reaction solution was heated at 80 °C for 15 minutes . The reaction solution was allowed to cool to room temperature, and the residue obtained by distilling off excess acetic anhydride under reduce pressure was added with aqueous sodium hydrogen carbonate, extracted with ethyl acetate. The organic layer was washed with water, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting residue was subjected to silica gel column chromatography (ethyl acetate :n-hexane=3:17), to obtain a mixture of the title compound and [3-(3-fluorophenyl)-l-trityl-2H-pyrazolo[4,3-b]pyridin-6-yl] acetate described in Production example 41 which was inseparable from the title compound as a colorless powder. Generated ratio: about 6:4, yield: 62 mg.
1H-NMR (400 MHz, CDCl3) δ 2.38 (3H, s) , 6.65 (1H, d, J = 9.1 Hz), 6.71 (1H, d, J = 9.1 Hz), 7.02 (1H, dt, J = 2.7, 8.0 Hz),
7.20-7.28 (6H, m) , 7.28-7.34 (9H, m), 7.38 (1H, dt, J = 5.8, 8.0 Hz), 8.10 (1H, ddd, J = 1.8, 2.7, 10.7 Hz), 8.19 (1H, bd, J = 8.0 Hz) . Production example 41
[3-(3-Fluorophenyl)-1-trityl-1H-pyrazolo[4,3-b]pyridin-6-yl] acetate
1H-NMR (400 MHz, CDCl3) δ 2.23 (3H, s), 6.38 (1H, d, J = 2.0 Hz) , 7.03 (1H, dt, J= 2.7, 8.0 Hz) , 7.20-7.28 (6H, m) , 7.28-7.34 (9H, m) , 7.4 0 (1H, dt, J = 5.8, 8.0 Hz) , 8.14 (1H, ddd, J = 1.8, 2.7, 10.7 Hz), 8.26 (1H, bd, J = 8.0 Hz), 8.36 (1H, d, J = 2.0 Hz) .
Example 4 2 3-(3-fluorophenyl)-1H-pyrazolo[4,3-b]pyridin-5-ol
62 mg of the mixture of [3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[4,3-b]pyridin-5-yl] acetate and [3-(3-fluorophenyl)-1-trityl-1H-pyrazolo[4,3-b]pyridin-6-yl] acetate obtained by Production example 40 was treated with the method in accordance with Production example 16, and purified and separated by silica gel column chromatography (ethyl acetate : n-hexane=l: 1-1: 0) , to obtain 10 mg of the title compound which is a high-polar component as a colorless powder and 10 mg of [3-(3-fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-6-yl] acetate as described in Example 43 below which is a low-polar component as a colorless powder.
1H-NMR (400 MHz, CD3OD) δ 6.60 (1H, d, J = 9.6 Hz), 7.15 (1H, dt, J=2.6, 8.0Hz), 7.52 (1H, dt, J=5.9, 8.0Hz), 7.56 (1H,
bd, J = 10.4 Hz), 7.62 (1H, bd, J = 8.0 Hz), 7.88 (1H, d, J = 9.6 Hz) . Example 43
[3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridin-6-yl] acetate 1H-NMR (400 MHz, CD3OD) δ 2.38 (3H, s), 7.12 (1H, dt, J = 2.7, 8.4 Hz), 7.49 (1H, dt, J = 6.1, 8.4 Hz), 7.80 (1H, d, J = 2.1Hz) , 8.21 (1H, ddd, J = 1.6, 2.7, 10.4 Hz), 8.27 (1H, dd, J = 1.6, 8.4 Hz), 8.42 (1H, d, J = 2.1Hz). Example 4 4
300 mg of 3-phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid obtained by Example 34 was dissolved in 5 mL of N,N-dimethylf ormamide solution, and each 0 . 5 mL was poured into 10 test tubes. Each test tube was added successively 130 µl of 1N amine solution in N,N-dimethylformamide, 130 µl of 1N 1-hydroxybenztriazole solution in 2V, N-dimethylf ormamide, 48 mg of l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and 90 µl diisopropylethylamine, subjected to sonication for 10 minutes, and left still all day and night. Each reaction solution was separated and purified by LC-MS[developing solvent: 0.1% trifluoroacetic acid-containing acetonitrile solution:0.1% trifluoroacetic acid-containing aqueous solution=l:99-100:0/20min.cycle, flowrate:20mL/mim: YMC Combiprep ODS-AM, 20mmΦ X 50mm (Long) ] , blown with nitrogen, and the solvent was removed, to afford the following test compounds.
Example 45
3-Phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid cyclopropylamide MS (ESI)m/z 279 MH+ Example 4 6
3-Phenyl-2H-pyrazolo[3,4-b]pyridine-5-carboxylic acid (pyridin-2-ylmethyl)-amide MS (ESI)m/z 330 MH+ Example 47
3-Phenyl-2H-pyrazolo [3, 4-b] pyridine-5-carboxylic acid (tetrahydrofuran-2-ylmethyl)-amide MS (ESI)m/z 323 MH+ Example 4 8
3-Phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI)m/z 319 MH+ Example 4 9
3-Phenyl-2H-pyrazolo [3, 4-b]pyridine-5-carboxylic acid (1-methoxymethyl-propyl)-amide MS (ESI)m/z 325 MH+ Example 50
3-Phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid (2-acetylamino-ethyl)-amide MS (ESI)m/z 324 MH+ Example 51 3-Phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid
(2-methylsulfanil-ethyl)-amide MS (ESI)m/z 313 MH+ Example 52
3-Phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid (l-hydroxymethyl-3-methylsulfanil-propyl)-amide MS (ESI)m/z 357 MH+ Example 53
3-Phenyl-2H-pyrazolo[3,4-b]pyridine-5-carboxylic acid (1-aza-bicyclo[2,2,2]octo-3-yl)-amide MS (ESI)m/z 348 MH+ Example 54
3-Phenyl-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid (trans-A-hydroxycyclohexyl)-amide MS (ESI)m/z 337 MH+ Example 55
3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic acid obtained by Example 7 was condensed with various kinds of amine in the same manner as described in Example 44, to afford the following test compounds. Example 56
3- (3-Fluorophenyl) -1H-pyrazolo [4, 3-b] pyridine-5-carboxylic acid cyclopropylamide MS (ESI)m/z 297 MH+ Example 57
3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI)m/z 337 MH+
Example 58
3-(3-Fluorophenyl)-2H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (tetrahydrofuran-2-ylmethyl)-amide
MS (ESI)m/z 340 MH+
Example 59
3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (2-acetylaminoethyl)-amide
MS (ESI)m/z 342 MH+
Example 60
3- (3-Fluorophenyl) -1H-pyrazolo [4, 3-b] pyridine-5-carboxylie
acid (pyridin-2-ylmethyl)-amide
MS (ESI)m/z 348 MH+
Example 61
3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (l-hydroxymethyl-2-methylpropyl)-amide
MS (ESI)m/z 343 MH+
Example 62
3-(3-Fluorophenyl)-1H-pyrazolo[4, 3-b]pyridine-5-carboxylie
acid (1-methoxymethylpropyl)-amide
MS (ESI)m/z 343 MH+
Example 63
3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylie
acid obtained by Example 17 was condensed with various kinds
of amine in the same manner as described in Example 44, to afford
the following test compounds.
Example 64
3-(Naphthalen-2-yl)-1H-pyrazolo[4, 3-b]pyridine-5-carboxylic
acid cyclopropylamide
MS (ESI)m/z 329 MH+
Example 65
3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (furan-2-ylmethyl)-amide
MS (ESI)m/z 369 MH+
Example 66
3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (tetrahydrofuran-2-ylmethyl)-amide
MS (ESI)m/z 373 MH+
Example 67
3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (2-acetylaminoethyl)-amide
MS (ESI)m/z 374 MH+
Example 68
3- (Naphthalen-2-yl) -1H-pyrazolo [4, 3-b] pyridine-5-carboxylic
acid (pyridin-2-ylmethyl)-amide
MS (ESI)m/z 380 MH+
Example 69
3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (l-hydroxymethyl-2-methylpropyl)-amide
MS (ESI)m/z 375 MH+
Example 7 0
3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridine-5-carboxylic
acid (1-methoxymethylpropyl)-amide
MS (ESI)m/z 375 MH+
Production example 71
5-Fluoro-4-(naphthalene-2-carbonyl)pyridine-2-carbonitrile
To a solution of 300 mg of (3-fluoro-l-oxypyridin-4-yl)naphthalen-2-yl-methanone obtained by Production example 27 in 3 mL of acetonitrile were added 0 . 30 mL of trimethylcyanide and 0 . 51 mL of dimethylcarbamyl chloride at room temperature, and heated under reflux for a day. The solution was added with water, extracted with ethyl acetate, washed with saturated brine, and then dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:3), to afford 11 mg of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ 7.60 (1H, ddd, J = 0.8, 7.2, 8.0 Hz), 7.68 (1H, ddd, J = 0.8, 7.2, 8.0 Hz), 7.88-8.00 (5H, m), 8.15 (1H, s), 8.75 (1H, s) . Production example 72 3-Fluoro-4-(naphthalene-2-carbonyl)pyridine-2-carbonitrile
From 300 mg of (3-fluoro-l-oxypyridin-4-yl)naphthalen-2-yl-methanone, 230 mg of the title compound was obtained as colorless crystals in accordance with the method of Production example 71. XH-NMR (400 MHz, CDCl3) δ 7.60 (1H, ddd, J = 0.8, 7.2, 8.0 Hz), 7.68 (1H, ddd, J = 0.8, 7.2, 8.0 Hz), 7.71 (1H, t, J= 4.8 Hz),
7.91 (1H, s), 7.93 (1H, d, J = 0.8 Hz), 7.95 (1H, dd, J = 1.6,
8.0 Hz) , 7.98 (1H, d, J=8.0Hz), 7.88-8.00 (5H, m) , 8.15 (1H,
s), 8.73 (1H, dd, 0.8, 4.8 Hz).
Example 7 3
5-Chloro-3-(naphthalen-2-yl)-1H-pyrazolo[3,4-c]pyridine
A solution of 500 mg of (2-chloro-5-fluoropyridin-4-yl)-(naphthalen-2-yl)-methanone obtained by Production example 28 in 5 mL pyridine was added at room temperature with 0.26 mL of hydrazine monohydrate, and heated under reflux for a day. The reaction solution was evaporated, and the resulting crystals were washed successively with water and ethyl acetate, toafford 350 mg of the title compound as pale yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 7.54 (1H, td, J =1.6, 7.2 Hz), 7.57 (1H, td, J=1.6, 7.2 Hz), 7.95 (1H, dd, J=1.6, 8.4 Hz), 8.03 (1H, d, J = 8.4 Hz), 8.17 (1H, d, J = 8.4 Hz), 8.20 (1H, dd, J = 1. 6, 8.4 Hz) , 8.4 2 (1H, d, J = 1.6 Hz) , 8.61 (1H, s) , 8.95 (1H, d, J = 1.6 Hz). Production example 74
5-Chloro-3-(naphthalen-2-yl)-1-trityl-lH-pyrazolo[3,4-c]pyridine
To a solution of 320 mg of 5-chloro-3-(naphthalen-2-yl)-lH-pyrazolo[3,4-c] pyridine obtained by Production example 73 in 3 mL of N,N-dimethylf ormamide was added 55 mg of sodium hydride at room temperature and stirred for 15 minutes at this temperature. Then
the solution was added with 335 mg of trityl-chloride at this temperature and stirred for 1 hour at this temperature. Then water was added and diluted with ethyl acetate. After washing the suspension in ethyl acetate twice with saturated aqueous ammonium chloride and once with water, crystals were collected by filtration. The crystals were washed with ethyl acetate, to afford 335 mg of the title compound as colorless crystals. 1H-NMR (400 MHz, DMSO-D6) δ 7.24-7.41 (15H, m) , 7.48 (1H, s) , 7.51-7.59 (2H, m), 7.92 (1H, d, J = 7.2 Hz), 7.96 (1H, d, J = 8.8 Hz), 7.99 (1H, d, J = 8.8 Hz), 8.15 (1H, d, J = 7.2 Hz), 8.46 (1H, s), 8.57 (1H, s). Example 7 5
3-(Naphthalen-2-yl)-lH-pyrazolo[3,4-c]pyridine-5-carbonitrile
From 11 mg of 5-fluoro-4-(naphthalen-2-carbonyl)pyridine-2-carbonitrile obtained by Production example 71, 9 mg of the title compound was obtained as colorless crystals in accordance with the method of Production example 3.
1H-NMR (4 00 MHz, CD3OD) δ 7.51-7.58 (2H, m) , 7.90-7.93 (1H, m) , 8.02 (1H, d, J = 8.8 Hz), 8.04-8.07 (1H, m), 8.15 (1H, dd, J = 1.6, 8.4 Hz), 8.51 (1H, s), 8.79 (1H, d, J = 1.6 Hz), 9.12 (1H, d, J = 1.6 Hz). Production example 7 6 (2-Chlorothiophen-3-yl)naphthalen-2-yl methanol
Under nitrogen atmosphere, to a solution of 3.2 mL of 1.57
M n-butyllithium in hexane in 7 mL of diethyl ether was added a solution of 1. 00 g of 3-bromo-2-chlorothiophene in 8 mL diethyl ether at -78°C, and stirred at this temperature for 1 hour. At the same temperature, 0.87 g of 2-naphthaldehyde was added, stirred for 3 hours while raising the temperature to room temperature, then addedwith water, extractedwith ethyl acetate, washed successively with saturated aqueous ammonium chloride and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:20-1:5), to afford 1.18 g of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 6.19 (1H, d, J = 3.6 Hz), 6.95 (1H, d, J= 6.0Hz), 7.05 (1H, dd, J = 0.4, 6.0 Hz), 7.4 3-7.50 (3H, m) , 7.78-7.85 (3H, m), 7.91 (1H, d, J = 0.4 Hz). Production example 77 (2-Chlorothiophen-3-yl)-(naphthalen-2-yl)-methanone
To a solution of 1.18 g of (2-chlorothiophen-3-yl)-(naphthalen-2-yl)-methanol in 12mLof toluene was added 1.12 g of activated manganese dioxide at room temperature, and after stirring at 50°C for a day, manganese dioxide was filtered off through Celite. The solvent was distilled off, and the resultant crude product was purified and separated by silica gel column chromatography (ethyl acetate : n-hexane=l: 50) , to afford 0. 61 g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 7.18 (1H, d, J = 6.0 Hz), 7.20 (1H,
d, J =6.0 Hz), 7.55 (1H, ddd, J =1.6, 7.2, 8.0 Hz), 7.61 (1H,
ddd, J = 1.6, 7.2, 8.0 Hz), 7.88-7.95 (3H, m), 7.96 (1H, dd,
J = 1.6, 8.0 Hz), 8.28 (1H, d, J = 0.8 Hz).
Example 78
3- (Naphthalen-2-yl)-lH-thieno[2,3-c]pyrazole
To a solution of 0.61 g of (2-chlorothiophen-3-yl)-(naphthalen-2-yl)-methanone in 6mL of ethanol was added 0.12 mL of hydrazine monohydrate at room temperature and heated under reflux for 2 days. The reaction solution was diluted with ethyl acetate, and the organic layer washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The resultant crude product was purified and separated by silica gel column chromatography (ethyl acetate : n-hexane=l: 30-1: 5) , to afford 32 mg of the title compound as colorless crystals.
1H-NMR (4 00 MHz, DMSO-D6) δ 7.37 (1H, d, J=5.6Hz), 7.51 (1H, td, J = 1.6, 8.0 Hz) , 7.55 (1H, td, J = 1.6, 8.0 Hz) , 7.57 (1H, d, J = 5.6 Hz) , 7.92 (1H, dd, J = 1.6, 8.0 Hz) , 7.98-8.09 (3H, m) , 8.42 (1H, s) . Production example 79 1,5-Dibromo-2,4-difluorobenzene
Under ice cooling, a solution of 25.35 g of l-bromo-2, 4-dif luorobenzene in 100 mL of concentrated sulfuric acid was added with 25.7 g of N-bromosuccinimide, and stirred
for 30 minutes at this temperature and for 2 days at room temperature. After cooling on ice, the reaction solution was added with ice, and extracted with 300 mL of diethyl ether. The organic layer was successively washed with water, saturated aqueous sodium hydrogen carbonate and saturated brine, and then dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting crude product was purified and separated by silica gel column chromatography (n-hexane), to afford 34.6 g of the title compound as a colorless oil. 1H-NMR ( 400 MHz, CDC13 ) 5 6.99 ( 1H, t, J = 8.4 Hz ) , 7.77 ( 1H, t, J = 6.8 Hz ) Production example 80 5-Bromo-2,4-difluoro-benzaldehyde
Under nitrogen atmosphere and at a temperature of -78°C, a solution of 34 . 0 g of 1, 5-dibromo-2, 4-dif luorobenzene obtained by Production example 79 in 250 mL of diethyl ether was added with 83 mL of 1.58 M .n-butyllithium in .n-hexane, and stirred at this temperature for 30 minutes. To the reaction solution, 12 mL of N,N-dimethylfor:mamide was added dropwise, and stirred at this temperature for 30 minutes. The reaction solution was added successively with 20 mL of glacial acetic acid and 300 mL of water, and extracted with diethyl ether. The organic layer was washed successively with 0.2N hydrochloric acid, water, saturated aqueous sodium hydrogen carbonate and saturatedbrine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting crude product was purified and
separated by silica gel column chromatography (n-hexane:ethyl
acetate =49:1) , to afford 21.4 g of the title compound as a pale
yellow oil.
1H-NMR ( 400 MHz, CDCl3) δ 7.03 ( 1H, dd, J = 8.0, 9.6 Hz ),
8.11 ( 1H t, J = 7.2 Hz ), 10.24 ( 1H, s )
Production example 81
2, 4-Difluoro-5-formyl-benzonitrile
To a solution of 21.4 g of 5-bromo-2,4-difluoro-benzaldehyde obtained by Production example 80 in 120 mL of N-methyl-2-pyrrolidone, 10.1 g of copper cyanide (I) was added and stirred at 175°C for 4 hours. After allowing the solution to cool, the reaction solution was added with water and diethyl ether, and the impurities were filtered off through Celite. The organic layer was washed successively with water (x3) and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting crude product was purified and separated by silica gel column chromatography (n-hexane:ethyl acetate =9:1), to afford 9.87 g of the title compound as pale yellow crystals. 1H-NMR ( 400 MHz, CDCl3) δ 7.14 ( 1H, dd, J = 8.4, 9.6 Hz ), 8.25 ( 1H t, J = 7.2 Hz ), 10.27 ( 1H, s ) Production example 82 6-Fluoro-lH-indazole-5~carbonitrile
2.50 g of 2, 4-difluoro-5-formyl-benzonitrile obtained by Production example 81 was dissolved in 15 mL of pyridine, added with 4.0 mL of hydrazine monohydrate and stirred at room
temperature for 2 hours. After evaporating the pyridine, the residue was dissolved in 200 mL of ethyl acetate and 40 mL of tetrahydrofuran, washed successively with IN hydrochloric acid, water, saturated aqueous sodium hydrogen carbonate and saturated brine, and dried over anhydrous magnesium sulfate. Aftermaking the organic layer pass through a silica gel pad (4 cm in diameter x 3 cm), the solvent was evaporated, to afford 1.63 g of the title compound as bright yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.66 ( 1H, d, J = 10. 0 Hz ) , 8.28 ( 1H, s ), 8.51 ( 1H, d, J = 6.0 Hz ), 13.68 ( 1H, s ) Production example 83 3-Bromo-6-fluoro-l-trityl-lH-indazole-5-carbonitrile
At room temperature, a solution containing 1.25 g of 6-fluoro-lH-indazole-5-carbonitrile obtained by Production example 82 in 12 mL of N,N-dimethylformamide was added with 1.52 g of N-bromosuccinimide and stirred overnight at this temperature. The reaction solution was added to 120 mL of ethyl acetate, and washed successively with semi saturated aqueous sodium hydrogen carbonate, water (twice) and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant residue was dissolved in 15 mL of N,N-dimethylformamide. Under ice cooling, 2.16 g trityl-chloride and 310 mg of 60% sodium hydride were successively added, and stirred at this temperature for 10 minutes, and further at room temperature for 20 minutes. After adding ice, to the reaction solution was extracted with 120 mL
of ethyl acetate. The organic layer was washed successively with water (twice) and saturated brine, and dried over anhydrous magnesium sulfate. After making the organic layer pass through a silica gel pad (4 cm in diameter x 3 cm) , the solvent was distilled off under reduced pressure, and the resultant crude crystals were recrystallized from ethyl acetate-diisopropyl ether, to afford 1.55 g of the title compound as pale yellow crystals. 1H-NMR (4 00 MHz, DMSO-D6) δ 6.17 (1H, d, J = 10.8 Hz) , 7.13-7.22 (6H, m) , 7.32 - 7.42 (9H, m) , 8.44 (1H, d, J = 6.4 Hz) Production example 84 2,4-Difluoro-3-formyl-benzonitrile
Under nitrogen atmosphere and ice cooling, to a solution of 11.1 g of N,N-diisopropylamine in 100 mL of tetrahydrofuran was added 66 mL of 1.6 M n-butyllithium in n-hexane and stirred at this temperature for 20 minutes. After cooling to -78°C, 15 mL of a solution containing 13 . 9 gof 2, 4-dif luorobenzonitrile in tetrahydrofuran was added dropwise. After stirring at this temperature for 10 minutes, 8 . 6 mL of A7,N-dimethylformamide was added dropwise and stirred at this temperature for 15 minutes. The reaction solution was added with 20 mL of glacial acetic acid, followed by 200 mL water, and extracted twice with diethyl ether. The organic layer was washed successively with 0.2N hydrochloric acid and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant crude crystals were triturated with diethyl ether n-hexane, to afford 8 . 61 g of the title compound as bright yellow
crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.53 ( 1H, t, J = 8.8 Hz ) , 8.33( 1H,
ddd, J = 6.0, 7.2, 8.8 Hz ), 10.17 ( 1H, s )
Production example 8 5
4-Fluoro-lH-indazole-5-carbonitrile
8.55 g of 2,4-difluoro-3-formyl-benzonitrile obtained by Production example 84 was dissolved in 40 mL of tetrahydrofuran and 40 mL of methanol, added with 5 .1 mL of hydrazine monohydrate, stirred at room temperature for 3 days, at 50°C for 3 hours, and at 70 °C for 3 hours. The reaction solution was added with 150 mL of ice water, further with 300 mL of ethyl acetate and 100 mL of tetrahydrofuran, and unnecessary substances were filtered out. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography (ethyl acetate : toluene =1: 9-1: 4) , to afford 509mg of the title compound as bright yellow crystals. Then the combined fraction with impurities was purified again by silica gel column chromatography (ethyl acetate :n-hexane=l: 4-1:0), to obtain 1. 80 g of the title compound as bright yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.58 ( 1H, d, J = 8.8 Hz ), 7.70 ( 1H, dd, J = 6.0, 8.8 Hz ), 8.45 ( 1H, s ), 13.94 ( 1H, s ) Production example 8 6 4-Fluoro-lH-indazole-5-carboxylic acid methyl ester
To 1. 65 g of 4-f luoro-lH-indazole-5-carbonitrile obtained
by Production example 85, 8 mL of glacial acetic acid, 8 mL of water and 16 mL of concentrated sulfuric acid were added and stirred at 110°C for 4 hours. After allowing the reaction solution to cool, 150 mL of ice water was added, and the precipitated carboxylic acid was collected by filtration. Under ice cooling, to a solution of the obtained carboxylic acid in N,N-dimethylformamide 12mL-tetrahydrofuran 40mL, a solution containing excess diazomethane in diethyl ether was added, and stirred at this temperature for 45 minutes. After distilling off the solvent under reduced pressure, the residue was dissolved in 100 mL of ethyl acetate, washed successively with saturated aqueous sodium hydrogen carbonate, water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 1.98 g of the title compound as bright yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.87 ( 3H, s ), 7.45 ( 1H, d, J = 8.8 Hz ), 7.82 ( 1H, dd, J = 6.8, 8.8 Hz ), 8.36 ( 1H, s ), 13.70 ( 1H, s ) Production example 87 3-Bromo-4-fluoro-lH-indazole-5-carboxylic acid methyl ester
At room temperature, to a solution of 2.2 g of 4-fluoro-lH-indazole-5-carboxylic acid methyl ester obtained by Production example 86 in 20 mL of N,N-dimethylformamide was added 2.12 g of N-bromosuccinimide and stirred at this temperature for 1 hour. After distilling off the solvent, the residue was added with 120 mL of ethyl acetate, and washed
2X0.

successively with semi-saturated aqueous sodium hydrogen
carbonate, water and saturated brine, and dried over anhydrous
magnesium sulfate. The solvent was evaporated, to afford 3.0
g of the title compound as bright yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.88 ( 3H, s ), 7.48 ( 1H, d, J
= 8.8 Hz ), 7.85 ( 1H, dd, J = 6.4, 8.8 Hz ), 14.00 ( 1H, s )
Production example 88
3-Bromo-4-fluoro-l-trityl-lH-indazole-5-carboxylic acid
methyl ester
Under ice cooling, to a solution of 2.99 g of 3-bromo-4-fluoro-lH-indazole-5-carboxylic acid methyl ester obtained by Production example 87 in 30 mL of tetrahydrofuran was added 526 mg of 60% sodium hydride, stirred for 25 minutes, added with 3.21 g of trityl chloride, and stirred at this temperature for 15 minutes and at room temperature for 4 5 minutes. The reaction solution was cooled again on ice, added with semi-saturated aqueous sodium hydrogen carbonate, and extracted with 100 mL of ethyl acetate. The organic layer was washed successively with water (twice) and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, the crude product purified and separated by silica gel column chromatography (ethyl acetate : n-hexane=l: 9) , and the obtained crude crystals were recrystallized from diisopropyl ether, to afford 1.73 g of the title compound as white needle crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.83 ( 3H, s ), 6.30 ( 1H, d, J = 8.8 Hz ), 7.12 - 7.20 ( 6H, m ), 7.30 - 7.40 ( 9H, m ), 7.55
( 1H, dd, J = 6.8, 8.8 Hz ) Production example 8 9 l-Bromo-4-fluoro-2-methoxybenzene
10 g of 2-bromo-5-fluorophenol was dissolved in 105 mL of N,N-dimethylformamide, and added with 10.9 g of potassium carbonate and 4 . 9 mL of iodomethane under cooling, and stirred at room temperature for 3 hours. The reaction solution was added with water, extracted with diethyl ether, and the resultant organic layer was washed with saturated brine, and dried over magnesium sulfate. The solvent was evaporated, to afford 9.75 g of the title compound as a yellow oil.
1H-NMR ( 400 MHz, CDCl3 } δ 3.88 ( 3H, s ), 6.59 ( 1H, td, J = 8.4, 2.8 Hz ), 6.65 ( 1H, dd, J = 10.4, 2.8 Hz ), 7.47 ( 1H, dd, J = 8.4, 6.0 Hz ) Production example 90 5-Bromo-2-fluoro-4-methoxybenzaldehyde
8.4 g of l-bromo-4-fluoro-2-methoxybenzene obtained by Production example 89 was dissolved in 200 mL of dichloromethane, added under nitrogen atmosphere at 0°C with 21 mL of titanium tetrachloride and 5.6 mL of dichloromethylmethylether, and stirred at room temperature for 4 hours and 30 minutes. The reaction solution was slowly poured into ice water, and extracted twice with diethyl ether. The organic layer was washed successively with water, saturated aqueous sodium hydrogen carbonate and water, and then dried over magnesium sulfate. The solvent was evaporated, to afford 9.44 g of the title compound
as white crystals.
1H-NMR ( 400 MHz, CDCl3 } δ 3.97 ( 3H, s ), 6.67 ( 1H, d, J =
12.0 Hz ),8.05 ( 1H, d, J = 7.6 Hz ), 10.15 ( 1H, s )
Production example 91
4-Fluoro-5-formyl-2-methoxybenzonitrile
5. 33 g of 5-bromo-2-fluoro-4-methoxybenzaldehyde obtained by Production example 90 was dissolved in 73 mL of l-methyl-2-pyrrolidone, added with 2.46 g of copper cyanide, and stirred at 180°C for 5 hours and 30 minutes. After allowing to cool, the reaction solution was added with water and diethyl ether, and insoluble substances were filtered off through Celite. The organic layer was washed with water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and purified and separated by silica gel column chromatography, to afford 0. 983 g of the title compound as pale yellow crystals. 1H-NMR ( 400 MHz, CDCl3 ) δ 4.03 ( 3H, s ), 6.76 ( 1H, d, J = 12.0 Hz ), 8.14 ( 1H, d, J = 7.2 Hz ), 10.17 ( 1H, s ) Production example 92 6-Methoxy-lH-indazole-5-carbonitrile
0.983 g of 4-fluoro-5-formyl-2-methoxybenzonitrile obtained by Production example 91 was dissolved in 15 mL of pyridine, and added with 2.66 mLof hydrazinemonohydrate. After stirring at 50°C for 2 hours, the solution was added with water and extracted twice with ethyl acetate. The resultant organic layer was washed with 1N hydrochloric acid, water, saturated aqueous sodium hydrogen carbonate and saturated brine, and dried
over magnesium sulfate. The solvent was evaporated, to afford 0.915 g of the title compound as pale yellow crystals. 1H-NMR ( 400 MHz, CD3OD } δ 3. 99 ( 3H, s ) , 7.10 ( 1H, s ) , 8.06 ( 1H, s ), 8.15 ( 1H, s ) Production example 93 3-Bromo-6-methoxy-lH-indazole-5-carbonitrile
To a solution of 0.915 g of 6-methoxy-lH-indazole-5-carbonitrile obtained by Production example 92 in 7.5 mL of N,N-dimethylformamide, 0.986 g of N-bromosuccinimide was added at room temperature, and stirred at this temperature for 1 hour and 30 minutes. The reaction solution was added with ethyl acetate, washed successively with semi-saturated aqueous sodium hydrogen carbonate, water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 1. 2 g of the title compound as yellow crystals.
1H-NMR ( 400 MHz, CD3OD) δ 4.00 ( 3H, s ), 7.10 ( 1H, s ), 7.97 ( 1H, s )
Production example 94 3-Bromo-6-methoxy-l-trityl-lH-indazole--5-carbonitrile
To a solution of 1.2 g of 3-bromo-6-methoxy-lH-indazole-5-carbonitrile obtained by Production example 93 in 50 mL of N,N-dimethylformamide were added 171 mg of sodium hydride and 1.6 g of triphenylmethane chloride under ice cooling, and stirred at this temperature for 1 hour and 30 minutes. The reaction solution was added with
water and extracted with ethyl acetate. The organic layer was washed successively with water and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, to afford 2.41 g of the title compound as brown crystals. 1H-NMR ( 400 MHz, CDC13 ) δ 3.36 ( 3H, s ) , 5. 60 ( 1H, s ) , 7.14 - 7.17 ( 5H, m ), 7.24 - 7.32 ( 10H, m ), 7.81 ( 1H, s ) Production example 95 4-Fluoro-2-methoxybenzonitrile
15 g of l-bromo-4-fluoro-2-methoxybenzene obtained by Production example 89 was dissolved in 150 mL of l-methyl-2-pyrrolidone, added with 9. 9 g of copper cyanide (I) , and stirred at 180°C for 5 hours. After allowing to cool, the reaction solution was poured into 500 mL of 14% ammonia aqueous solution, stirredfor 45minutes, then addedwith 150mLof diethyl ether, and stirred for another 10 minutes. After filtering off the insoluble substances through Celite, the solution was extracted with diethyl ether. The organic layer was washed with diluted aqueous ammonia, 5N hydrochloric acid, water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, to afford 10.1 g of the title compound as white crystals.
1H-NMR ( 400 MHz, CDC13 ) δ 3.93 ( 3H, s ), 6.67 - 6.74 ( 2H, m ), 7.54 ( 1H, dd, J = 6.0, 8.4 Hz ) Production example 96 4-Fluoro-3-formyl-2-methoxybenzonitrile
Under nitrogen atmosphere and at -78°C, to a solution of
10. 7 g of N,N-diisopropylamine in 150 mL of tetrahydrofuran was added 40 mL of 2.66 M n-butyllithium in n-hexane, stirred at this temperature for 1 hour and 15 minutes, and added dropwise with a solution of 14.5 g of 4-fluoro-2-methoxybenzonitrile obtained by Production example 95 in 50 mL of tetrahydrofuran. After stirring at this temperature for 2 hours, 11.94 g of N-formylpiperidine was added. After stirring at this temperature for 40 minutes, the solution was added with 20 mL of acetic acid at this temperature, added with water at room temperature, and extracted three times with diethyl ether. The organic layer was washed successively with 0.2N hydrochloric acid, water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography, to afford 6.4 g of the title compound 6.4 g as pale yellow crystals.
1H-NMR ( 400 MHz, CDCl3 ) δ 4.22 ( 3H, s ), 6.99 ( 1H, t, J = 8.8 Hz ), 7.78 ( 1H, dd, J = 5.6, 8.8 Hz ), 10.34 ( 1H, s ). Production example 97 4-Methoxy-1H-indazole-5-carbonitrile
From 6.4 g of 4-fluoro-3-formyl-2-methoxybenzonitrile, 6.03 g of the title compound was obtained as yellow crystals in accordance with the method of Production example 92. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 4.37 ( 3H, s ), 7.20 ( 1H, d, J = 8.8 Hz ), 7.45 ( 1H, d, J = 8.8 Hz ), 8.57 ( 1H, s ), 13.61 ( 1H, bs )
Production example 98 3-Bromo-4-methoxy-1H-indazole-5-carbonitrile
From 72 mg of 4-methoxy-lH-indazole-5-carbonitrile, 69 mg of the title compound was obtained as white crystal in accordance with the method of Production example 93.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 4.15 ( 3H, s ), 7.42 ( 1H, d, J = 8.8 Hz ), 7.59 ( 1H, d, J = 8.8 Hz ), 13.94 ( 1H, bs ) Production example 99 3-bromo-4-methoxy-l-trityl-1H-indazole-5-carbonitrile
From 69 mg of 3-bromo-4-methoxy-lH-indazole-5-carbonitrile, 150 mg of the title compound was obtained as a brown oil in accordance with the method of Production example 94.
1H-NMR ( 400 MHz, CDCl3 ) δ 4.29 ( 3H, s ), 6.11 ( 1H, d, J = 8.8 Hz ), 7.00 ( 1H, d, J = 8.8 Hz ), 7.11 - 7.32 ( 15H, m ) Example 100
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carbonitrile
To a solution of 1.35 g of 3-bromo-6-fluoro-l-trityl-1H-indazole-5-carbonitrile obtained by Production example 83 in 9 mL of N,N-dimethylformamide were successively added 685 mg of 3-fluoro-styrene,167mg of 2-(di-tert-butylphosphino)biphenyl, 63 mg of palladium acetate (II) and 1.95 mL of triethylamine, and stirred at 80°C for 2 hours. The solvent was evaporated, to obtain 2.75 g of a crude coupling compound. The obtained
crude coupling compound was suspended in 9 mL of dichloromethane, added with 3 mL of trifluoroacetic acid and 0.5 mL triisopropylsilane, and stirred at room temperature for 4 hours . The reaction solution was poured into a stirred and mixed solution of 100 mL saturated aqueous sodium hydrogen carbonate/90 mL ethyl acetate/10 mL tetrahydrofuran. The organic layer was washed successively with saturated aqueous sodium hydrogen carbonate and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, the crude product purified and separated by silica gel column chromatography (ethyl acetate :toluene =1:19-1:9), to afford 645 mg of the title compound as bright yellow crystals.
1H-NMR ( 400 MHz, CD3-OD ) δ 7.08 ( 1H, dt, J = 2.0, 8.4 Hz ), 7.44 ( 1H, dt, J = 5.6, 7.6 Hz ), 7.48 ( 1H, d, J = 9.6 Hz ), 7.48 -7.54 (2H, m ), 7.56 ( 1H, d, J = 16.4 Hz ), 7.62 ( 1H, d, J = 16.4 Hz ), 8.71 ( 1H, d, J = 6.0 Hz ) Example 101
6-Fluoro-3-[(£)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxylic acid
To a suspension of 544 mg of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carbonitrile obtained by Example 100 in 2 mL of acetic acid and 3 mL of water was added 6 mL of concentrated sulfuric acid, and stirred at 110°C for 12 hours. After allowing to cool, the reaction solution was added with ice, and extracted with a mixed solvent of 200 mL of ethyl acetate and 50 mL of tetrahydrofuran. The organic layer
was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 491 mg of the title compound as pale brown crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.12 ( 1H, dt, J = 2.0, 8.4 Hz ) , 7.41 ( 1H, d, J = 11.2 Hz ), 7.42 ( 1H, dt, J = 5.6, 7.6 Hz ), 7.53 ( 1H, d, J = 16.8 Hz ), 7.57 ( 1H, d, J = 8.0 Hz ), 7.69 ( 1H, d, J = 10.8 Hz ), 7.76 ( 1H, d, J = 16.8 Hz ), 8.75 ( 1H, d, J =, 6.8 Hz ), 13.02 ( 1H, br s), 13.50 ( 1H, s ). Compounds of Examples 102-h to 107-h were synthesized according to the following synthesizing method.
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxylic acid synthesized in Example 101 was dissolved in N,N-dimethylformamide and dispensed into test tubes. Each test tube was added successively with preliminarily prepared 1.5 equivalents of different kind of amine in 1M dimethylformamide solution, 2 equivalents of 1-hydroxybenztriazole monohydrate in 1 M dimethylformamide solution, and 2 equivalents of l-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (=WSC-HC1), and stirred at room temperature overnight. The resultant reaction solution was purified and separated by LC-MS[developing solvent: 0.1% trifluoroacetic acid-containing acetonitrile solution:0.1% trifluoroacetic acid-containing aqueous solution=20:80-80:20, 10 min. cycle, flow rate: 30 mL/min., column: Wakopak Combi ODs, 20 mm $ x 50 mm(Long) ] , to afford the compounds of the following Examples. Example 102
6-Fluoro-3-[{E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 340 MH+
Example 103
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 380 MH+
Example 104
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid (tetrahydrofuran-2-ylmethyl)-amide
MS (ESI) m/z 384 MH+
Example 105
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid [(IS)-l-hydroxymethyl-2-methylpropyl]-amide
MS (ESI) m/z 386 MH+
Example 106
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid (pyridine-3-ylmethyl)-amide
MS (ESI) m/z 391 MH+
Example 107
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-car
boxylic acid [(1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 763 2M+Na+
Production example 108
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-
indazole-5-carboxylic acid
Under ice cooling, to a solution of 350 mg of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxylic acid obtained by Example 101 in 6 mL of N,N-dimethylformamide was added 103 mg of 60% sodium hydride, stirred for 30 minutes, then added with 390 mg of trityl chloride, and stirred at this temperature for 20 minutes and at room temperature for 1 hour. The reaction solution was added with water and extracted with 80 mL of ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography (ethyl acetate : toluene =1: 9) , to afford 370 mg of the title compound as bright yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 5.95 ( 1H, d, J = 12. 4 Hz ) , 7.05 -7.40 ( 18H, m ), 7.50 ( 1H, d, J = 7.6 Hz ), 7.66 ( 1H, d, J = 10.4 Hz ), 7.68 ( 1H, d, J= 16.4 Hz ), 8.72 ( 1H, d, J=7.2 Hz ), 13.16 ( 1H, br s ) Production example 109
{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-1H-indazol-5-yl}carbamic acid tert-butyl ester
To a suspension of 369 mg of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-l-trityl-1H-indazole-5-carboxylic acid obtained by Production example 108 in 6 mL of toluene were added 69 mg of triethylamine, 0.15 mL of 2-methyl-2-propanol and 187 mg of diphenyl phosphoryl azide, and stirred at 80°C for 7.5 hours. After adding 10 mL of ethyl acetate to the reaction
solution, the organic layer was washed successively with water
and saturated brine, and dried over anhydrous magnesium sulfate.
The solvent was evaporated, and the crude product purified and
separated by silica gel column chromatography (n-hexane: toluene
=1:1), to afford 102 mg of the title compound as a white
non-crystalline powder.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.44 ( 9H, s ), 5.99 ( 1H, d, J
= 11.6 Hz), 7.11 (1H, dt, J = 2.0, 8.0 Hz), 7.16-7.45 (17H,
m ), 7.50 ( 1H, d, J = 7.6 Hz ), 7.56 ( 1H, d, J = 16.8 Hz ),
7.63 ( 1H, d, J = 10.4 Hz ), 8.21 ( 1H, d, J = 7.6 Hz ), 8.86
( 1H, s )
Example 110
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-
ylamine
A solution of 96 mg of {6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-l-trityl-lH-indazol-5-yl}carbamic acid obtained by Production example 109 in a solution of tert-butyl ester dissolved in 1 mL of 95% trifluoroacetic acid was stirred at room temperature for 1 hour and 20 minutes. The reaction solution was added with saturated aqueous sodium hydrogen carbonate, andextractedwith 20mLof ethyl acetate. Theorganic layer was washed successively with saturated aqueous sodium hydrogen carbonate and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, the crude product purified and separated by silica gel column chromatography (ethyl acetate :toluene=l:1), to afford 28 mg
of the title compound as a bright yellow non-crystalline powder. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 4. 92 ( 2H, s ) , 7.07 ( 1H, dt, J = 2.0, 8.0 Hz ), 7.22 ( 1H, d, J = 11.2 Hz ), 7.28 ( 1H, d, J = 16.8 Hz ), 7.38 ( 1H, d, J = 8.8 Hz ) , 7.37 ( 1H, dt, J = 6.0, 8.0 Hz ), 7.45 ( 1H, d, J = 7.6 Hz ), 7.49 ( 1H, d, J = 16.8 Hz ), 7.51 ( 1H, d, J = 10.4 Hz ), 12.86 ( 1H, s )
Compounds of Examples 111-114 were synthesized in the following synthesizing method.
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-ylamine synthesized in Example 110 was dissolved in N,N-dimethylf ormamide and dispensed into test tubes . Each test tube was added successively with preliminarily prepared 1.2 equivalents of different kind of carboxylic acid in 1M dimethylformamide solution, 1.6 equivalents of 1-hydroxybenztriazole monohydrate in 1 M dimethylformamide solution, and 1.6 equivalents of l-ethyl-3-(3'-dimethylaminopropyl) carbodiimide hydrochloride (=WSC-HC1), and stirred at room temperature for 3 days. The resultant reaction solution was purified and separated by LC-MS[developing solvent: 0.1% trifluoroacetic acid-containing acetonitrile solution:0.1% trifluoroacetic acid-containing aqueous solution=20:80-80:20, 10 min. cycle, flow rate: 30 mL/min., column: Wako Wakopak Combi ODS, 20 mm Φx 50 mm (Long)], to afford the compounds of the following Examples. Example 111
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(3-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 340 MH+
Example 112
(25)-5-oxo-pyrrolidine-2-carboxylic acid {6-fluoro-3-
[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide
MS (ESI) m/z 383 MH+
Example 113
N-{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2- (thiophen-2-yl)-acetamide
MS (ESI) m/z 396 MH+
Example 114
Furan-2-carboxylic acid {6-fluoro-3-[(E)-2-(3-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 366 MH+
Example 115
6-Fluoro-3- [ (E) -2- (3-fluorophenyl) -vinyl] -lH-indazole-5-
carboxylic acid amide
To a suspension of 89 mg of 6-fluoro-3-[(E)-2-(3-
fluorophenyl)-vinyl]-lH-indazole-5-carbonitrile obtained by
Example 100 in 0.4 mL of acetic acid and 0.4 mL of water was
added 1. 2 mL of concentrated sulfuric acid, and stirred at 110°C
for 50 minutes. After allowing to cool, ice was added, and
extracted with a mixed solvent of 20 mL of ethyl acetate and
10 mL of tetrahydrofuran. The organic layer was washed
successively with water, saturated aqueous sodium hydrogen

carbonate and saturated brine, and dried over anhydrous magnesium sulfate. After making the organic layer pass through an alumina pad (2.5 diameter x 2 cm) , the solvent was evaporated, to afford 7 6 mg of the title compound as pale yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.12 ( 1H, dt, J = 2.0, 8.4 Hz ), 7.39 ( 1H, d, J = 10.8 Hz ), 7.41 ( 1H, dt, J = 6.0, 8.4 Hz ), 7.53 ( 1H, d, J = 16.8 Hz ), 7.56 ( 1H, d, J = 8.0 Hz ), 7.61 ( 1H, s ), 7.66 ( 1H, d, J = 10.8 Hz ), 7.70 ( 1H, d, J = 16.8 Hz ), 7.74 ( 1H, s ), 8.50 ( 1H, d, J =, 6.8 Hz ), 13.40 ( 1H, s)
Production example 116 (6-Bromo-2,3-difluorophenyl)trimethylsilane
Under nitrogen atmosphere, to a solution of 18.2 mL of N,N-diisopropylamine in 200 mL of tetrahydrof uran was added 66. 0 mL of 1.57 M n-butyllithium in hexane at 0°C, and stirred at this temperature for 10 minutes. After cooling to -78°C, a solution containing 20.0 g of l-bromo-3,4-difluorobenzene in 100 mL of tetrahydrof uran was added dropwise and stirred at this temperature for 30 minutes, stirred at this temperature for 30 minutes, added dropwise with 32.9 mL of chlorotrimethylsilane, and the reaction solution was allowed to warm gradually to room temperature, and stirred for a day. After diluting with water and ethyl acetate, the organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (n-hexane), to afford 20.3
g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 0.47 (9H, s) , 6.99 (1H, dt, J = 9.6,
8.8 Hz), 7.27 (1H, ddd, J = 2.0, 4.0, 8.8 Hz).
Production example 117
5-Bromo-2,3-difluoro-4-trimethylsilanyl benzaldehyde
Under nitrogen atmosphere, to a solution containing 6.34 mL of N,N-diisopropylamine in 100 mL of tetrahydrofuran, 26.4 mL of 1. 57 M n-butyllithium in hexane was added at 0 °C, and stirred at this temperature for 10 minutes. After cooling to -78°C, a solution containing 10.0 g of
(6-bromo-2,3-difluorophenyl)trimethylsilane in 100 mL of tetrahydrofuran was added dropwise, stirred at this temperature for 1 hour, then added dropwise with 2.92 mL of N,N-dimethylformamide, allowed to gradually warm to room temperature, and stirred fro 3 hours . After diluting with water and ethyl acetate, the organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (n-hexane) , to afford 9.70 g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 0.50 (9H, s), 7.77 (1H, dd, J = 4.8, 2.0 Hz), 10.27 (1H, s). Production example 118 5-Bromo-2,3-difluoro benzaldehyde
To a solution of 18.0 g of 5-bromo-2,3-difluoro-4-trimethylsilanyl benzaldehyde in 20 mL of N,N-dimethylf ormamide
and 2 mL of water was added 1.63 g of cesium fluoride at room
temperature, and stirred at this temperature for 1 hour. After
diluting with ethyl acetate, the organic layer was washed
successively with saturated aqueous ammonium chloride and
saturated brine, dried over anhydrous magnesium sulfate, and
the solvent was evaporated, to afford 10 . 26 g of the title compound
as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ7.58 (1H, ddd, J =9.2, 6.8, 2.4 Hz),
7.76 (1H, ddd, J = 4.8, 2.4, 2.0 Hz), 10.27 (1H, s).
Production example 119
3,4-Difluoro-5-formylbenzonitrile
To a solution of 10 . 0 g of 5-bromo-2, 3-difluorobenzaldehyde in 4 0.0 mL of l-methyl-2-pyrrolidone was added 4.2 6 g of copper cyanide (I) at room temperature and stirred at 170°C for 8 hours. Adding ethyl acetate and water, the solution was stirred, and insoluble substances were filtered out through celite. The organic layer of filtrate was washed successively with saturated aqueous ammonium chloride, water and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:20) , to afford 2.64 g of the title compound as colorless crystals. 1H-NMR (400MHz, CDC13)δ7.72 (1H, ddd, J =9.2, 6.8, 2.0Hz), 7.98 (1H, dt, J = 5.6, 2.0 Hz), 10.32 (1H, s). Production example 120 7-Fluoro-lH-indazole-5-carbonitrile
From 2.60 g of 3, 4-difluoro-5-formyl benzonitrile, 2.46 g of the title compound was obtained as pale yellow crystals according to the method of Production example 92. 1H-NMR (400 MHz, DMSO-D6) δ7.68 (1H, dd, J =11.2, 0.8 Hz), 8.27 (1H, d, J = 0.8 Hz), 8.37 (1H, d, J = 3.2 Hz). Production example 121 3-Bromo-7-fluoro-1H-indazole-5-carbonitrile
From 2.40 g of 7-fluoro-lH-indazole-5-carbonitrile, 2.91 g of the title compound was obtained as pale red crystals according to the method of Production example 93.
1H-NMR (400 MHz, DMSO-D6) δ7.84 (1H, dd, J = 10.8, 0.8 Hz) , 8.13 (1H, d, J = 0.8 Hz). Production example 122 3-Bromo-7-fluoro-l-trityl-lH-indazole-5-carbonitrile
From 2.91 g of 3-bromo-7-fluoro-lH-indazole-5-carbonitrile, 2.08 g of the title compound was obtained as colorless crystals according to the method of Production example 94.
1H-NMR (4 00MHz, CDCl3) δ 7.01 (1H, dd, J =10.8, 0.8 Hz) , 7.09-7.32 (15H, m), 7.82 (1H, d, J = 0.8 Hz). Production example 123 7-Fluoro-l-trityl-3-vinyl-2H-indazole-5-carbonitrile
Under nitrogen atmosphere, 3.32 g of 3-bromo-7-fluoro-l-trityl-1H-indazole-5-carbonitrile was dissolved in 100 mL of toluene, added with 398 mg of tetrakis(triphenylphosphilne)palladium(0) and 2.50 mL of
vinyltributyltin, and stirred at 100°C for 95 minutes. After cooling to room temperature, 10 g of silica gel was added, the solvent was evaporated, followed by purification by silica gel column chromatography, to afford 2.59 g of the title compound as white crystals.
1-NMR (400 MHz, CDCl3) δ 5.55 (1H, dd, J =0.8, 11.2 Hz), 6.00 (1H, dd, J = 0.8, 18.0 Hz), 6.8 6-6.97 (2H, m) , 7.10-7.30 (15H, m), 8.07 (1H, d, J = 1.2 Hz). Production example 124
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-indazole-5-carbonitrile
Under nitrogen atmosphere, 2.59 g of 7-fluoro-l-trityl-3-vinyl-1H-indazole-5-carbonitrile was dissolved in 50 mL of acetonitrile, added with 10 mL of triethylamine, 271 mg of palladium acetate (II), 450 mg of 2-(di-tert-butylphosphino)biphenyl and 726 µl of 3-bromo pyridine, and refluxed overnight. The solution was cooled to room temperature, added with 10 g of silica gel, the solvent was evaporated, followed by purification by silica gel column chromatography, to afford 2. 00 g of the title compound as yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 7.09 (6H, d, J = 6.4 Hz) , 7.27-7.33 (9H, m), 7.40 (1H, dd, J = 4.8, 8.0 Hz), 7.52 (1H, d, J = 16.8 Hz) , 7.56 (1H, dd, J = 1.2, 13.2 Hz) , 7.62 (1H, d, J = 16.8 Hz), 8.17 (1H, m), 8.48 (1H, dd, J = 1.6, 4.8 Hz), 8.84 (1H, d, J = 1.2 Hz), 8.87 (1H, d, J = 1.6 Hz).
Production example 125
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-
indazole-5-carboxylic acid
3.60 g of 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-indazole-5-carbonitrile was dissolved in 500 mL of ethanol, added with 154 g of potassium hydroxide, and stirred at 80°C overnight. After cooling to room temperature, the solution was adjusted to pH 3 by 5N hydrochloric acid, and extracted with ethyl acetate. The organic layer was washed with water and saturated brine, dried over magnesium sulfate, and the solvent was evaporated. The obtained crude product was purified by silica gel column chromatography, and the resultant dark brown solid was washed with 10 mL of diethyl ether, to afford 3.58 g of the title compound as dark brown crystals. 1H-NMR (400 MHz, DMSO-D6) δ 7.11 (6H, d, J =7.2 Hz), 7.26-7.35 (9H, m) , 7.40 (1H, d, J = 16.8 Hz) , 7.42 (1H, dd, J = 1.2, 12.4 Hz) , 7.63 (1H, m) , 7.85 (1H, d, J = 16.8 Hz) , 8.4 7 (1H, m) , 8.59 (1H, d, J = 5.2 Hz), 8.68 (1H, d, J= 1.2 Hz), 9.00 (1H, bs). Example 126
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl] -l-trityl-1H-indazole-5-carboxylic acid
By treating 1.00 g of 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-l-trityl-2H-indazole-5-carboxylic acid in the manner similar to that described in Example 16, 741 mg of the title compound was obtained as yellow crystals. 1H-NMR (400 MHz, DMSO-D6) 6 7.56 (1H, m), 7.60 (1H, d, J =16.8
Hz), 7.67 (1H, d, J= 12.4 Hz), 7.91 (1H, d, J= 16.4 Hz), 8.39 (1H, m), 8.55 (1H, d, J = 4.8 Hz), 8.66 (1H, s), 8.99 (1H, s) . Example 127
7-Fluoro-3-[{E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
376 mg of 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid was dissolved in 10 mL of N,N-dimethylformamide, and added with 903 µl of N,N-diisopropylethylamine, 244 mg of 1-hydroxybenzotriazole monohydrate and 147 µl of furfurylamine, and stirred at room temperature for 10 minutes. The solution was cooled to 0°C, added with 509 mg of l-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, and stirred at room temperature overnight. After diluting with water and ethyl acetate, the organic phase was washed with water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the resultant crude product was purified by silica gel column chromatography, to afford 159 mg of the title compound as yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 4.53 (2H, d, J = 6.0 Hz), 6.32 (1H, d, J = 2.8 Hz), 6.41 (1H, dd, J = 2.0, 3.2 Hz), 7.43 (1H, dd, J=4.8, 7.2Hz), 7.58 (1H, d, J= 16.8 Hz), 7.59 (1H, s), 7.71 (1H, d, J = 12.0), 7.72 (1H, d, J = 16.8 Hz), 8.18 (1H, d, J = 8.0 Hz), 8.49 (1H, d, J = 4.8 Hz), 8.56 (1H, s), 8.88 (1H, d, J = 2.4 Hz), 9.07 (1H, bs). Example 128
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide
By treating 200 mg of 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-2H-indazole-5-carboxylic acid obtained by Example 126 and 94.5 ul L-valinol in accordance with Example 127, 102 mg of the title compound was obtained as white crystals. 1H-NMR (400 MHz, DMSO-D6) δ 0.94 (6H, t, J =6.4 Hz), 1.96 (1H, m), 3.56 (2H, t, J = 5.6 Hz), 4.10 (1H, t, J = 4.8 Hz), 4.63 (1H, m) , 7.4 3 (1H, dd, J = 4.8, 7.2 Hz) , 7.51-7.68 (2H, m) , 7.74 (1H, d, J = 16.8 Hz), 8.05 (1H, m) , 8.16 (1H, m) , 8.46 (1H, s) , 8.48 (1H, s), 8.86 (1H, s). MS (ESI) m/z 369 MH+ Example 12 9
7-Fluoro-3-[(EZ)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid [ (1S)-1-carbamoylethyl]-amide
By treating 200 mg of 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid obtained by Example 126 and 106 mg of (2S)-2-amino-propioneamide hydrochloride in accordance with Example 127, 132 mg of the title compound was obtained as a mixture of ElZ=3/1.
(E) compound: 1H-NMR (400 MHz, DMSO-D6) δ 1.38 (3H, d, J = 7.2 Hz), 4.46 (1H, m), 7.44 (1H, dd, J = 4.8, 8.0 Hz), 7.58 (1H, d, J = 16.8 Hz), 7.70 (1H, d, J = 12.4 Hz), 7.74 (1H, d, J = 16.4 Hz), 8.18 (1H, d, J = 7.6 Hz), 8.48 (1H, dd, J = 1.6, 4.4 Hz), 8.51 (1H, s), 8.87 (1H, s) . (Z) compound: 1H-NMR (400 MHz, DMSO-D6) δ 1.30 (3H, d, J = 7.2
Hz) , 4.26 (1H, m) , 6.84 (1H, m) , 7.06 (1H, d, J = 12.8 Hz) , 7.32 (1H, dd, J = 5.2, 8.0 Hz), 7.92 (1H, d, J = 8.4 Hz) , 8.06 (1H, s), 8.08 (1H, s), 8.34 (1H, dd, J= 1.9, 5.1 Hz), 8.77 (1H, s).
MS (ESI)m/z 354 MH+
Example 130
From 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-
indazole-5-carboxylic acid obtained by Example 126 and various
kinds of amine, compounds of Examples 131-132 were obtained in
accordance with the method of Example 102.
Example 131
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-
carboxylic acid [(IS)-2-hydroxy-l-phenylethyl]-amide
MS (ESI)m/z 403 MH+
Example 132
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI)m/z 323 MH+
Production example 133
{7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-
indazol-5-yl}-carbamic acid tert-butyl ester Under nitrogen atmosphere, 200 rng of
7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-
indazole-5-carboxylic acid obtained by Production example 125
was dissolved in toluene, added with 79.6 µl of triethylamine
and 81.8 µL of diphenylphosphoryl azide, and stirred at room
temperature for 30 minutes. Then after adding 1 mL of
tert-butanol, the reaction solution was heated to 120°C and
stirred for 95 minutes. After cooling to room temperature, 1
g silica gel was added, the solvent was evaporated, and purified
by silica gel column chromatography, to afford 121 mg of the
title compound as a yellow solid solution.
1H-NMR (400 MHz, DMSO-D6) δ 1.47 (9H, s), 7.10 (6H, d, J = 6.8
Hz), 7.23 (1H, d, J = 8.0 Hz), 7.25-7.33 (10H, m), 7.39 (1H,
dd, J = 5.2, 8.0 Hz), 7.51 (1H, d, J = 16.8 Hz) , 8.03 (1H, s),
8.13 (1H, m), 8.46 (1H, dd, J = 1.6, A.A Hz), 8.75 (1H, d, J
= 2.0), 9.44 (1H, bs).
Example 134
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-
ylamine
By treating 655 mg of {7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazol-5-yl}-carbamic acid tert-butyl ester by the method in accordance with Example 16, 153 mg of the title compound was obtained as a dark brown solid solution.
1H-NMR(400 MHz, DMSO-D6) δ 5. 11 (2H, bs) , 6. 65 (1H, d, J = 13. 6 Hz), 6.98 (1H, s), 7.29 (1H, d, J = 17.6 Hz), 7.40 (1H, dd, J = 5.2, 8.0 Hz), 7.56 (1H, d, J = 16.8 Hz), 8.10 (1H, m), 8.44 (1H, dd, J = 1.6, 4.8 Hz), 8.79 (1H, s), 13.3 (1H, bs). Production example 135 Furan-2-carboxylic acid
{7-fluoro-1-(furan-2-carbonyl)-3-[(E)-2-(pyridin-3-yl)-viny 1]-1H-indazole-5-yl}-amide
Following amidation using 217 mg of 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-ylamine and 286 mg of 2-f urancarboxylic acid in accordance with the method of Example 127, purification by LC-MS was carried out, to afford 143 mg of the title compound as yellow crystals. MS (ESI)m/z 443 MH+ Example 136
Furan-2-carboxylic acid [7-fluoro-3-(2-pyridin-3-yl-vinyl)-lH-indazol-5-yl]-amide
208 mg of furan-2-carboxylic acid {7-fluoro-1-(furan-2-carbonyl)-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-amide was suspended in 20 mL ethanol, added with 5 mL of concentrated aqueous ammonia and stirred at room temperature overnight. The reaction solution was added with saturated brine, extracted with ethyl acetate, and the organic phase was washed with saturated brine, and dried over magnesium sulfate. The solvent was evaporated, purified by LC-MS and the obtained crystals were suspended in ethyl acetate, neutralized with saturated aqueous sodium hydrogen carbonate, and the organic phase was washed with water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, to afford 96.2 mg of the title compound as yellow crystals. MS (ESI)m/z 349 MH+ Production example 137
2-[(E)-2-(3-Fluorophenyl)-vinyl]-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane
To a solution of 11 nag of chloro (1,5-cyclooctadiene)-rhodium(I) dimer and 0.61 mL of 4,4,5,5-tetramethyl-[1,3,2]dioxaborolane in 15 mL toluene was added dropwise 1.0 mL of 3-fluorostyrene, and stirred at room temperature for 4 hours. The reaction solution was added with water, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant crude product was purified and separated by silica gel column chromatography (diethyl ether : n-hexane=l: 19) , to afford 311 mg of the title compound as a pale yellow oil. 1H-NMR (400 MHz, DMSO-D6) δ 1.25 (12H, s), 6.22 (1H, d, J =18.8 Hz), 7.16 (1H, dt, J = 2.4, 8.8 Hz), 7.30 (1H, d, J= 18.8 Hz), 7.37-7.52 (3H, m). Production example 138
{4-[(E)-2-(4,4,5,5,-tetramethyl-[1,3,2]dioxaborolane-2-yl)-vinyl]-phenyl}-carbamic acid tert-butyl ester
From 0.921 g of (4-vinylphenyl)-carbamic acid tert-butyl ester, 0.41 g of the title compound was obtained as white crude crystals in accordance with the method of Production example 137.
1H-NMR (400 MHz, CDCl3)δ 1.30 (12H, s), 1.51 (9H, s), 6.04 (1H, d, J = 17.6 Hz) , 6.52 (1H, bs) , 7.32 (2H, d, J = 8.8 Hz) , 7.33 (1H, d, J = 17.6 Hz), 7.41 (2H, d, J = 8.8 Hz). Production example 139
2-[(E)-2-(2-Fluorophenyl)-vinyl]-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane
From 5 g of 1-fluoro-2-vinylbenzene, 4.22 g of the title
compound was obtained as a yellow oil in accordance with the
method of Production example 137.
1H-NMR (400 MHz, CDCl3) δ 1.31 (12H, s), 6.23 (1H, d, J =18.0
Hz), 6.97-7.07 (1H, m), 7.07-7.14 (1H, m), 7.18-7.29 (1H, m),
7.55 (1H, dt, J = 2.0, 8.0 Hz), 7.57 (1H, d, J = 18.0 Hz).
Production example 14 0
6-Fluoro-lH-indazole-5-carboxylic acid methyl ester
From 1.22 g of 6-fluoro-lH-indazole-5-carbonitrile obtained by Production example 82, 1.11 g of the title compound was obtained as ocher crystal in accordance with the method of Production example 86.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.86 (3H, s) , 7.43 (1H, d, J = 11.6 Hz), 8.26 (1H, s), 8.44 (1H, d, J = 7.2 Hz), 13.43 (1H, s). Production example 141
3-Bromo-6-fluoro-l-trityl-lH-indazole-5-carboxylic acid methyl ester
From 1.11 g of 6-fluoro-lH-indazole-5-carboxylic acid methyl ester, 1.38 g of the title compound was obtained as pale red crystal in accordance with Production example 87 and Production example 88.
1H-NMR (400 MHz, DMSO-D6) δ 3.84 (3H, s), 6.04 (1H, d, J =12.0 Hz), 7.12-7.23 (6H, m), 7.30-7.44 (9H, m), 8.12 (1H, d, J = 6.8 Hz) .
Example 142
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid methyl ester
In accordance with the method of Example 100, from 800 mg of 3-bromo-6-fluoro-l-trityl-lH-indazole-5-carboxylic acid methyl ester and 350 mg of 2-vinylthiophene, were obtained 211 mg of the title (E)-compound as bright yellow crystals and 85 mg of (Z)-compound of Example 143 as bright yellow crystals. (E) compound: 1H-NMR (4 00 MHz, DMSO-D6) δ 3.85 (3H, s), 7.11 (1H, dd, J=3.6, 5.2 Hz), 7.32 (1H, d, J= 16.4 Hz), 7.41 (1H, d, J = 3.6Hz), 7.44 (1H, d, J = 11.2 Hz), 7.54 (1H, d, J=5.2 Hz), 7.71 (1H, d, J = 16.4 Hz), 8.7 0 (1H, d, J = 6.8 Hz), 13.51 (1H, s) . Example 14 3
6-Fluoro-3-[(Z)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid methyl ester
(Z) compound: 1H-NMR (400 MHz, DMSO-D6) δ 3.85 (3H, s), 6.82 (1H, d, J = 12.0 Hz) , 7.03 (1H, d, J = 12.0 Hz) , 7.0 6 (1H, dd, J = 3.6, 5.2 Hz), 7.44 (1H, d, J = 11.6Hz), 7.50 (1H, d, J = 5.2 Hz), 7.63 (1H, d, J = 3.6 Hz), 8.34 (1H, d, J = 6.8 Hz), 13.63 (1H, s) . Example 14 4
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid
To a mixed solution of 60 mg of 6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acidmethyl ester obtained by Example 142 in a mixture of 2 mL tetrahydrofuran/0. 5 mL methanol was added 0.5 rnL of 5N sodium hydroxide aqueous solution, and stirred at 50°C-55°C for
6 hours. The reaction solution was made acidic with IN hydrochloric acid, and extracted with 15 mL of ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 53 mg of the title compound as bright yellow crystals.
1H-NMR (4 00 MHz, DMSO-D6) δ 7.11 (1H, dd, J = 3.2, 5.2 Hz), 7.31 (1H, d, J = 16.0 Hz), 7.39 (1H, d, J = 11.2 Hz), 7.41 (1H, d, J = 3.2Hz), 7.54 ( 1H, d, J = 5.2 Hz ), 7.69 (1H, d, J = 16.0 Hz), 8.67 (1H, d, J=7.2 Hz), 13.07 (1H, bs), 13.46 (1H, s). Example 145
In accordance with the method of Example 102, from 6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 146-151 were obtained. Example 14 6
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 328 MH+ Example 147
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 368 MH+ Example 14 8
6-Fluoro-3-t(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid [(15)-(2-hydroxy-l-phenyl-ethyl)]-amide
MS (ESI) m/z 408 MH+
Example 14 9
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid [(IS)-(l-hydroxymethyl-2-methyl-propyl)]-
amide
MS (ESI) m/z 374 MH+
Example 150
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI) m/z 379 MH+
Example 151
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid [(IS)-(1-carbamoyl-ethyl)]-amide
MS (ESI) m/z 359 MH+
Production example 152
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1-trityl-lH-
indazole-5-carboxylic acid
151 mg of 6-fluoro-3-t(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid methyl ester obtained by Example 142 was tritylated in the same manner as described in Production example 22, followedby hydrolysis in the same manner as described in Example 144, to afford 320 mg of the title compound as bright yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 5.99 (1H, d, J =12.0 Hz), 7.11 (1H, dd, J = 3.2, 5.2 Hz), 7.14-7.48 (17H, m), 7.54 (1H, d, J = 5.2 Hz), 7.59 (1H, d, J = 16.4 Hz), 8.66 (1H, d, J= 6.8Hz), 13.18
(1H, bs).
Production example 153
{6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1-trityl-lH-
indazol-5-yl}-carbamic acid tert-butyl ester
From 320 mg of 6-fluoro-3-[(E)-2-thiophen-2-yl-vinyl] -l-trityl-lH-indazole-5-carboxylic acid, 66 mg of the title compound was obtained as orange crystals in the similar method as described in Production example 109. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.44 (9H, s) , 6.00 (1H, d, J = 11.6 Hz), 7.09 (1H, dd, J = 2.8, 5.6 Hz), 7.14-7.55 (19H, m), 8.15 (1H, d, J = 7.6 Hz), 8.85 (1H, s). Example 154
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazol-5-ylamine
From 90 mg of {6-fluoro-3-[(E)-2-(thiophen-2-yl) -vinyl]-l-trityl-1H-indazol-5-yl}-carbamic acid tert-butyl ester, 22 mg of the title compound was obtained as a dark brown powder in the similar method as described in Example 110. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 4.94 (2H, s), 7.09 (1H, dd, J = 3.6, 5.2 Hz), 7.12 (1H, d, J = 16.4 Hz), 7.22 (2H, d, J = 10.8 Hz), 7.25 (1H, d, J = 3.6Hz), 7.33 (1H, d, J = 8.4 Hz), 7.44 (1H, d, J = 16.4 Hz), 7.47 (1H, d, J=5.2Hz), 12.83 (1H, s). Example 155
From 6-f luoro-3- [ (E) -2- (thiophen-2-yl) -vinyl] -1H-indazol-5-ylamine and various kinds of carboxylic acid, compounds of Examples 156-159 were obtained in accordance with
the method of Example 111. Example 156
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(thiophen-2-yl) -vinyl] -lJJ-indazol-5-yl} -amide MS (ESI) m/z 328 MH+ Example 157
(2S)-5-Oxo-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H- indazol-5-yl}-amide MS (ESI) m/z 371 MH+ Example 158
N-{6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazol-5-yl}-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 384 MH+ Example 159
Furan-2-carboxylic acid {6-fluoro-3-
[{E)-2-(thiophen-2-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 354 MH+ Example 160
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1H-indazole-5-carbonitrile
From 1.0 g of 3-bromo-6—fluoro-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 83 and 674 mg of 2-vinylnaphthalene, 590 mg of the title compound was obtained as ocher crystals in accordance with the method of Example 100. 1H-NMR (400 MHz, DMSO-D6) δ 7.47-7.58 (2H, m), 7.67 (1H, d, J
= 10.0 Hz), 7.72 (1H, d, J = 16.8 Hz), 7.83 (1H, d, J = 16.4
Hz), 7.93 (2H, d, J = 9.2 Hz), 7.96 (1H, d, J = 8.8 Hz), 8.01
(1H, d, J = 8.4 Hz), 8.20 (1H, s), 9.06 (1H, d, J = 6.4 Hz),
13.76 (1H, s).
Example 161
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid
To a suspension of 588 mg of 6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-lH-indazole-5-carbonitrile in 3 mL of acetic acid and 1 mL of water was added 3 mL of concentrated sulfuric acid, and stirred at 110°C for 1 hours. After allowing to cool, the reaction solution was added with ice, and extracted with amixed solvent of 30mL ethyl acetate/15mL tetrahydrofuran. The organic layer was washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant crude product was dissolved in 6 mL of 1,4-dioxane, added with 6 mL of 4N lithium hydroxide, and stirred at 120°C for 15 hours. The reaction solution was made acidic with 5N hydrochloric acid, and then extracted with amixed solvent of 30 mL ethyl acetate/20 mL tetrahydrofuran. The organic layer was washed successively with water and saturatedbrine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 477 mg of the title compound as ocher crystals. 1H-NMR (400 MHz, DMSO-D6) δ 7.41 (1H, d, J = 11.6 Hz) , 7.48-7.57 (2H, m), 7.70 (1H, d, J = 16.4 Hz), 7.82 (1H, d, J = 16.4 Hz), 7.92 (1H, d, J = 8.8 Hz), 7.94 (2H, d, J = 8.8 Hz), 8.05 (1H,
d, J = 8.4 Hz), 8.17 (1H, s), 8.79 (1H, d, J = 7.2 Hz), 13.08
(1H, bs), 13.49 (1H, s) . Example 162
From 6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 163-166 were obtained in accordance with the method of Example 102. Example 163
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 372 MH+ Example 164
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 412 MH+ Example 165
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid (pyridin-3-ylmethyl)-amide MS (ESI) m/z 423 MH+ Example 166
6-Fluoro-3-[{E)-2-(naphthalen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid [(1S)-(1-carbamoyl-ethyl)]-amide MS (ESI) m/z 403 MH+ Production example 167
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1-trityl-lH-indazole-5-carboxylic acid
From 350 mg of 6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid obtained by Example 161, 188 mg of the title compound was obtained as bright yellow non-crystalline powder in accordance with Production example 108.
1H-NMR (4 00 MHz, DMSO-D6) δ 6.00 (1H, d, J = 12.4 Hz), 7.10-7. 44
(15H, m), 7.48-7.56 (2H, m), 7.58 (1H, d, J = 16.4 Hz), 7.75
(1H, d, J = 16.4 Hz), 7.88-7.98 (3H, m), 8.04 (1H, d, J = 8.8
Hz), 8.13 (1H, s), 8.79 (1H, d, J = 7.2 Hz), 13.19 (1H, bs).
Production example 168
{6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1-trityl-1H-indazol-5-yl}-carbamic acid tert-butyl ester
From 182 mg of 6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-l-trityl-lH-indazole-5-carboxylic acid, 42 mg of the title compound was obtained as a yellow viscous oil in the similar method as described in Production example 109. 1H-NMR (400 MHz, DMSO-D6) δ 1.46 (9H, s) , 6.02 (1H, d, J =11.2 Hz), 7.10-7.58 (18H, m), 7.62 (1H, d, J = 16.4 Hz), 7.85-7.95 (3H, m), 7.99 (1H, d, J = 8.4 Hz), 8.13 (1H, s), 8.26 (1H, d, J = 7.2 Hz), 8.88 (1H, s) . Example 169
6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl] -1H-indazole-5-ylamine
From 42 mg of {6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-l-trityl-lH-indazol-5-yl}-carbamic acid tert-butyl ester, 14 mg of the title compound was obtained as ocher crystals
in the similar method as described in Example 110.
1H-NMR (400 MHz, DMSO-D6) δ 4.96 (2H, s) , 4.23 (1H, d, J =10.0
Hz), 7.40-7.56 (4H, m), 7.57 (1H, d, J = 16.8 Hz), 7.85-7.97
(4H, m), 8.04 (1H, s), 12.86 (1H, s).
Example 170
From 6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-1H-indazol-5-ylamine and various kinds of carboxylic acid, compounds of Examples 171-173 were obtained in accordance with the method of Example 111. Example 171
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 372 MH+ Example 172
(2S)-5-Oxo-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 415 MH+ Example 173
N-{6-Fluoro-3-[(E)-2-(naphthalen-2-yl)-vinyl]-lH-indazol-5-yl}-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 428 MH+ Production example 174
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-l-trityl-1H-indazol-5-ylamine
To a solution of 2.95 g of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-l-trityl-lH-indazole-5-carboxylic acid
obtained by Production example 108 in 55 mL of N,N-dimethylformamide, 1.58 g of potassium carbonate and 1.50 g of diphenyl phosphoryl azide were added and stirred at room temperature for 30 minutes followed by stirring at 40-50°C for 2.5hours. After adding water, the reaction solution was allowed to cool to room temperature, and extracted with 200 mL of ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resulting crude product was purified and separated by silica gel column chromatography (n-hexane:toluene =1:1-1:2), to afford 1.57 g of the title compound as yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 5.00 (2H, s), 5.91 (1H, d, J =12.4 Hz), 7.09 (1H, t, J = 8.0 Hz), 7.14-7.48 (19H, m) , 7.54 (1H, d, J = 10.4 Hz). Example 175
N-{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-yl}-acetamide
525 mg of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine and 80 µl of glacial acetic acid were dehydraing-condensed in accordance with Example 127, followed by deprotection in the similar method as described in Example 16, to afford 150 mg of the title compound as bright yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 2.11 (3H, s), 7.15 (1H, dt, J =2.4, 8.0 Hz) , 7.38-7.4 8 (3H, m) , 7.52 (1H, d, J = 8.0 Hz), 7.58-7.66
(2H, m), 8.4 0 (1H, d, J = 7.6 Hz), 9.72 (1H, s), 13.2 6 (1H, s) .
Production example 176
N- (5-Fluoro-2-methyl-phenyl)-acetamide
To a solution of 5 g of 5-fluoro-2-methylaniline in 30 mL of pyridine was added 4.6 mL of acetic anhydride, and stirred at room temperature for 4 hours. After distilling off the pyridine under reduced pressure, the residue was dissolved in 250 mL of ethyl acetate, and washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogen carbonate and saturated brine. The solution was dried over anhydrous magnesium sulfate, allowed to pass though a silica gel pad, and then the solvent was evaporated. The residue was recrystallized from ethyl acetate -diethyl ether, to afford 5 . 34 g of the title compound as white needle crystals. The mother liquor was further concentrated, and triturated with diethyl ether, to afford 907 mg of the title compound as purple needle crystals.
1H-NMR (400 MHz, CDCl3) δ 2.22 (3H, s) , 6.76 (1H, ddd, J = 2.0, 7.2, 8.0 Hz) , 7.00 (1H, bs) , 7.11 (1H, t, J = 7.2 Hz) , 7.77 (1H, dd, J = 2.0, 6.8 Hz). Production example 177 N- (5-Fluoro-2-methyl-4-nitrophenyl)-acetamide
To a solution of 5.01 g of N- (5-fluoro-2-methyl-phenyl)-acetamide in 30 mL of concentrated sulfuric acid was added dropwise 2.74 gof70%nitric acid (d=1.42) while keeping the inner temperature at 3°C. After
stirring for 40 minutes at this temperature, the reaction solution was poured dropwise into ice water under stirring. After collecting the precipitated powder by filtration, the powder was dissolved in 100 mL of ethyl acetate, washed successively with saturated aqueous sodium hydrogen carbonate(twice) and saturated brine. After drying over anhydrous magnesium sulfate, the solvent was evaporated. The residue was crystallized from ethanol, to afford 3.68 g of the title compound as yellow needle crystals.
1H-NMR (400 MHz, CDCl3) δ 2.29 (3H, s) , 2.32 (3H, s) , 7.17 (1H, bs), 7.94 (1H, d, J = 8.0 Hz), 8.34 (1H, d, J = 13.6 Hz). Production example 178 5-Fluoro-2-methyl-4-nitro-phenylamine
A suspension of 3.5 g of N- (5-fluoro-2-methyl-4-nitrophenyl)-acetamide in 5N hydrochloric acid was heated under reflux for 1 hour. After allowing to cool, the solution was neutralized with potassium carbonate, and extractedwith 80mLof ethyl acetate . Theorganic layer was dried over anhydrous magnesium sulfate, allowed to pass through a silica gel pad, and then the solvent was distilled off under reduced pressure, to afford 2 . 69 g of the title compound as ocher crystals.
1H-NMR (400 MHz, CDCl3) δ 2.16 (3H, s) , 4.39 (2H, bs) , 6.39 (1H, d, J = 12.8 Hz), 7.88 (1H, d, J = 8.4 Hz). Production example 17 9 6-Fluoro-5-nitro-lH-indazole
To a suspension of 2 g of 5-fluoro-2-methyl~4-nitro-phenylamine in 50 mL of glacial acetic acid, an aqueous solution of 812 mg of sodium sulfite was added, and stirred at room temperature overnight. The reaction solution was added with 100 mL of water, the precipitated powder filtered out, and the filtrate was concentrated to 50 mL. After extracting with 100 mL of ethyl acetate, the organic layer was washed successively with aqueous sodium hydrogen carbonate, 10% sodium thiosulfate aqueous solution, water and saturated brine, dried over anhydrous magnesium sulfate, and then the solvent was evaporated. The obtained crude crystals were triturated with toluene, to afford 589 mg of the title compound as bright yellow crystals.
1H-NMR (4 00 MHz, DMSO-D6) δ 7.69 (1H, d, J = 11.6 Hz) , 8.39 (1H, s), 8.79 (1H, d, J = 7.2 Hz), 13.76 (1H, s). Production example 180 3-Bromo-6-fluoro-5-nitro-l-trityl-lH-indazole
In accordance with Production example 87 and Production example 88, from 500 mg of 6-fluoro-5-nitro-lH-indazole, 651 mg of the title compound was obtained as pale brown crystals. 1H-NMR (400 MHz, DMSO-D6) δ 6.20 (1H, d, J= 12.4 Hz), 7.15-7.23 (6H, m) , 7.33-7.43 (9H, m) , 8.47 (1H, d, J = 7.2 Hz). Production example 181
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-nitro-l-trityl-lH-indazole
To a solution of 600 mg of
3-bromo-6-fluoro-5-nitro-l-trityl-lH-indazole in 6 mL of N,N-dimethylformamide were successively added 292 mg of 4-fluorostyrene, 71 mg of 2-(di-tert-butylphosphino)biphenyl, 27 mg of palladium acetate (II) and 0.85 mL of triethylamine, and stirred at 80°C for 6.5 hours. The solvent was evaporated, and the resulting residue was dissolved in 25 mL of ethyl acetate. The organic layer was successively washed with water (twice) and saturated brine, dried over anhydrous magnesium sulfate, and then the solvent was evaporated. The obtained crude crystal was recrystallized from ethyl acetate-diisopropylether, to afford 304 mg of the title compound as bright yellow crystals. 1H-NMR (400 MHz, DMSO-D6) δ 6.12 (1H, d, J = 12.8 Hz), 7.15-7.30 (8H, m) , 7.32-7.4 6 (9H, m) , 7.47 (1H, d, J = 16.4 Hz) , 7.63 (1H, d, J = 16.4 Hz), 7.81 (1H, dd, J = 5.6, 8.4 Hz), 9.16 (1H, d, J = 6.8 Hz) . Production example 182
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine
To a solution of 290 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-nitro-l-trityl-lH-indazole in a mixture of 5 mL N,N-dimethylf ormamide/9 mL methanol/1 mL water were added 30 mg of ammonium chloride and 150 mg of iron powder, and stirred at 80°C for 8 hours. After filtering off the insoluble substances through Celite, the solvent was evaporated, and the resultant residue was dissolved in 15 mL of ethyl acetate . The organic layer was washed successively with water, saturated
aqueous sodium hydrogen carbonate and saturated brine. After drying over anhydrous magnesium sulfate, the solvent was evaporated, to afford 252 mg of the title compound as pale brown non-crystalline powder.
1H-NMR (400 MHz, DMSO-D6) δ 4.98 (2H, s), 5.91 (1H, d, J =12.0 Hz), 7.14-7.24 (8H, m), 7.25-7.37 (11H, m), 7.40 (1H, d, J = 8.8 Hz), 7.69 (1H, dd, J = 5.6, 8.8 Hz). Example 183
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-1H-indazol-5-ylamine synthesized in Production example 182 was dissolved in N,N-dimethylformamide, and dispensed into test tubes . Each test tube was added successively withpreliminarily prepared 1.5 equivalents of different kind of carboxylic acid in 1M dimethylformamide solution, 1.5 equivalents of 1-hydroxybenztriazole monohydrate in 1 M dimethylformamide solution, and 1.5 equivalents of
l-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (=WSC-HC1), and stirred at room temperature for 6 days. After adding semi-saturated sodium hydrogen carbonate to each test tube, the solution was extracted with ethyl acetate. After distilling off the solvent, the residue was dissolved in 10% trifluoroacetic acid/2% tripropylsilane/dichloromethane and stirred for 6.5 hours. After adding 5% water/methanol to each test tube, the solvent was distilled off. The residue was dissolved in N,N-dimethylformamide, and purified and separated by LC-MS [developing solvent: 0.1% trifluoroacetic
acid-containing acetonitrile solution:0.1% trifluoroacetic
acid-containing aqueous solution=20:80-80:20, 10 min. cycle,
flow rate: 30 mL/min., column: Wako Wakopak Combi ODS, 20 mm
Φ x 50 mm(Long)], to afford the compounds of Examples 184-188.
Example 18 4
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 340 MH+
Example 185
N-{ 6-Fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-2-(thiophen-2-yl)-acetamide
MS (ESI) m/z 396 MH+
Example 18 6
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-acetamide
MS (ESI) m/z 314 MH+
Example 187
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-3-hydroxy-2,2-dimethyl-propanamide
MS (ESI) m/z 372 MH+
Example 188
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-3-hydroxy-2-phenyl-propanamide
MS (ESI) m/z 420 MH+
Example 18 9
C-{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-methylamine
To a suspension of 5 mg of lithium aluminum hydride in 1 mL of tetrahydrofuran was added 13 mg of aluminum chloride (III), followed by 9 mg of 6-fluoro-3-[(E) -2-(3-fluorophenyl)-vinyl] -1H-indazole-5-carbonitrile obtained by Example 100, and stirred at room temperature overnight. After adding saturated aqueous ammonium chloride, the reaction solution was added with 15 mL of ethyl acetate. The organic layer was washed successively with saturated aqueous sodium hydrogen carbonate(twice) and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography (methanol: chloroform=l: 9) , to afford 3 mg of the title compound as a yellow oil.
1H-NMR (400 MHz, DMSO-D6) δ 3.86 (2H, s) , 7.12 (1H, t, J= 8.4Hz) , 7.29 (1H, d, J= 10.0 Hz), 7.44 (1H, dt, J=6.0, 8.0 Hz), 7.52 (1H, d, J = 16.8 Hz), 7,54 (1H, d, J = 8.8 Hz), 7.59 (1H, d, J = 16.8 Hz), 7.61 (1H, d, J = 10.8 Hz), 8.22 (1H, d, J = 7.2 Hz), 13.19 (1H, s) . Example 190
N-{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-ylmethyl}-2-methoxy-benzamide
From 2.9 mg of C-{6-fluoro-3-[(E) -2-(3-fluorophenyl)-vinyl]-lH-indazol-5-yl}-methylamine, 1.42 mg of the title compound was obtained in accordance with the method of Example 111.
MS (ESI) m/z 420 MH+ Production example 191
6-Fluoro-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid methyl ester
0.77 g of 3-bromo-6-fluoro-l-trityl-1H-indazole-5-carboxylic acid methyl ester obtained by Production example 141 was treated in the method of Production example 123, to obtain 600 mg of the title compound as colorless needle crystals. 1H-NMR (400 MHz, CDCl3) δ 3.90 (3H, s), 5.52 (1H, d, J = 11.5 Hz), 6.05 (1H, d, J = 12.4 Hz), 6.08 (1H, d, J = 18.2 Hz), 6.94 (1H, dd, J = 12.4, 18.2 Hz), 7.14-7.22 (6H, m), 7.24-7.32 (9H, m) , 8.51 (1H, d, J = 6.9 Hz). Production example 192
6-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole-5-carboxylic acid methyl ester
600 mg of 6-fluoro-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid methyl ester and 190 ul of 3-bromopyridine were treated in the same manner as described in Example 124, to afford 75 mg of the title compound as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 3.93 (3H, s) , 6.11 (1H, d, J = 11.9 Hz), 7.15-7.25 (6H, m), 7.25-7.42 (12H, m), 7.89 (1H, dt, J= 1.8, 8.3 Hz), 8.51 (1H, dd, J = 1.8, 4.7 Hz), 8.59 (1H, d, J = 6.8 Hz), 8.76 (1H, d, J = 1.8 Hz). Example 193 N-[6-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-
yl]-2- (thiophen-2-yl)-acetamide
75 mg of 6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-indazole-5-carboxylic acid methyl ester was alkaline hydrolyzed in the same manner as described in Example 144, to give 70 mg of 6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-indazole-5-carboxylic acid as a colorless powder. Then in the same manner as described in Example 174, 30 mg of 6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazol-5-ylamine was obtained. Further, in the same manner as described in Example 175, this compound was subjected to dehydration condensation with 10 mg of 2-thiopheneacetic acid, followed by deprotection, to obtain 8 mg of the title compound as a colorless powder.
1H-NMR (400MHz, CD3OD) δ 4.02 (2H, s), 7.00 (1H, dd, J= 3.5, 4.7 Hz) , 7.06 (1H, dd, J = 1.0, 3.5 Hz), 7.32 (1H, dd, J = 1.0, 4.7 Hz), 7.34 (1H, d, J = 11.5 Hz), 7.45 (1H, dd, J = 4.7, 8.0 Hz) , 7.4 7 (1H, d, J = 16.3 Hz) , 7.56 (1H, d, J = 16.3 Hz) , 8.15 (1H, dt, J=1.8, 8.0 Hz), 8.43 (1H, dd, J = 1.8, 4.7 Hz), 8.57 (1H, d, J = 6.8 Hz), 8.76 (1H, d, J = 1.8 Hz). Example 194
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-car boxylic acid methyl ester
To a solution of 150 mg of 3-bromo-4-fluoro-1-trityl-lH-indazole-5-carboxylic acid methyl ester obtained by Production example 88 in 6 mL of N,N-dimethylformamide were successively added 200 mg of 2-[(E)-2-(3-fluorophenyl)-vinyl]-4,4,5,5-
tetramethyl-[1,3,2]dioxaborolane obtained by Production example 137, 24 mg of 2-(di-tert-butylphosphino)biphenyl, 9 mg of palladium acetate (II), 117 mg of potassium fluoride and 0. 6 mL of water, and stirred 80°C for 1.5 hours. The reaction solution was added with 30 mL of ethyl acetate, and the organic layer was washed successively with water, semi-saturated brine and saturated brine and dried over anhydrous magnesium sulfate. The solvent was evaporated, to give 260 mg of a crude coupling compound. Then the crude coupling compound was deblocked in the manner as described in Example 6, to afford 32 mg of the title compound as bright yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ3.89 (3H, s), 7.15 (1H, dt, J =2.4, 8.4 Hz) , 7.42-7.62 (6H, m), 7.84 (1H, dd, J = 6.4, 8.8 Hz), 13.81 (1H, s). Example 195
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxylic acid
From 35 mg of 4-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl] -1H-indazole-5-carboxylic acid methyl ester, 31 mg of the title compound was obtained as ocher crystals in accordance with the method of Example 144.
1H-NMR (4 00MHz, DMSO-D6) δ 7 .15 (1H, dt, J =2. 4, 8.4 Hz) , 7.38-7.60 (6H, m), 7.83 (1H, dd, J= 6.4r 8.8 Hz), 13.08 (1H, bs), 13.76 (1H, s). Example 196
From 4-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-
indazole-5-carboxylic acid and various kinds of amine, compounds
of Examples 197-202 were obtained in accordance with the method
of Example 102.
Example 197
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-car
boxylic acid cyclopropylamide
MS (ESI) m/z 340 MH+
Example 198
4-Fluoro-3-[(E)-2-(3-fluorophenyl) -vinyl]-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 380 MH+
Example 199
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 396 MH+
Example 200
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid [(IS)-l-hydroxymethyl-2-methyl-propyl]-amide
MS (ESI) m/z 386 MH+
Example 201
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-
carboxylic acid [(IS)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 763 2M+Na+
Example 202
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-
carboxylic acid [(1S)-1-(2-methoxy-ethylcarbamoyl)-ethyl]-
amide
MS (ESI) m/z 429 MH+
Production example 203
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-
indazole-5-carboxylic acid
From 72 mg of 4-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxylic acid methyl ester obtained by Example 194, 45 mg of the title compound was obtained as yellow crystals in accordance with the method of Production example 152. 1H-NMR (400 MHz, DMSO-D6) δ 6.23 (1H, d, J =8.8 Hz), 7.10-7.60 (22H, m), 13.14 (1H, bs). Production example 204
4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine
From 45 mg of 4-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-l-trityl-lH-indazole-5-carboxylic acid, 4 mg of the title compound was obtained as a dark brown powder in accordance with the method of Production example 174.
1H-NMR (400 MHz, DMSO-D6) δ4. 90 (2H, s) , 5.95 (1H, d, J = 8.4 Hz), 6.63 (1H, t, J = 8.4 Hz), 7.06-7.48 (21H, m). Example 205
N-{4-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-carboxylic acid}-2-(thiophen-2-yl)-acetamide
From 4 mg 4-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine and 2-thiopheneacetic acid, 0.83 mg of the title compound was obtained in accordance with the
method of Example 183. MS (ESI) m/z 396 MH+ Production example 206 4-Fluoro-3-iodo-lH-indazole-5-carbonitrile
To a solution of 161 mg of 4-fluoro-1H-indazole-5-carbonitrile obtained by Production example 85 in 4 mL of N,N-dimethylformamide was added 285 mg of iV-iodosuccinimide was added, and stirred at 75°C for 4 hours. The reaction solution was added with 40 mL of ethyl acetate, then washed successively with semi-saturated aqueous sodium hydrogen carbonate, water, semi-saturated brine and saturated brine, and dried over anhydrous magnesium sulfate. The resultant crude product was purified and separated by silica gel column chromatography (ethyl acetate:toluene =1:9), to afford 273 mg of the title compound as white crystals. 1H-NMR (400 MHz, DMSO-D6) δ7.60 (1H, d, J = 8.4 Hz), 7.16 (1H, dd, J = 6.0, 8.4 Hz), 14.23 (1H, s) . Production example 207 4-Fluoro-3-iodo-l-trityl-lH-indazole-5-carbonitrile
From 250 mg of 4-fluoro-3-iodo-1H-indazole-5-carbonitrile, 247 mg of the title compound was obtained as white crystals in accordance with the method of Production example 88. 1H-NMR (400 MHz, DMSO-D6) δ6.34 (1H, d, J = 8.8 Hz), 7.08-7.16 (6H, m), 7.30-7.39 (9H, m), 8.47 (1H, dd, J = 6.4, 8.8 Hz). Example 208 4-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-
carbonitrile
In accordance with the method of Example 100, from 235 mg of 4-fluoro-3-iodo-l-trityl-lH-indazole-5-carbonitrile and 98 mg of 2-vinylthiophene, 11 mg of (2)-compound described in Example 209 was obtained as bright yellow crystal and further 79 mg of the title (E)-compound was obtained as bright yellow crystals.
[E) compound: 1H-NMR (400 MHz, DMSO-D6) δ7.12 (1H, dd, J =3.6, 5.2 Hz), 7.14 (1H, d, J = 16.4 Hz), 7.38 (1H, d, J = 3.6Hz), 7.54 (1H, d, J = 8.8 Hz), 7.56 (1H, d, J = 5.2 Hz), 7.70 (1H, d, J = 16.4 Hz) , 7.71 1H, dd, J = 6.0, 8.8 Hz), 13.99 (1H, s). Example 209
4-Fluoro-3- [ (2) -2- (thiophen-2-yl) -vinyl] -1-trityl-1H-indazole-5-carbonitrile
(2) compound: 1H-NMR (400 MHz, DMSO-D6) δ6.71 (1H, d, J = 12.0 Hz), 7.07 (1H, dd, J=4.0, 5.2 Hz), 7.10 (1H, d, J= 12.0 Hz), 7.51 (1H, d, J = 5.2 Hz), 7.57 (1H, d, J = 8.8 Hz), 7.59 (1H, d, J=4.0Hz), 7.71 (1H, dd, J = 6.0, 8.8 Hz), 14.09 (1H, s). Example 210
4-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid
A suspension of 25 mg of 4-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carbonitrile in 2 mL of 4N lithium hydroxide was heated under reflux for 9 hours. After allowing to cool, 2N hydrochloric acid was added to make acidic, and extracted with 15 mL of ethyl acetate. The organic layer was
washed with water and saturated brine. After drying over anhydrous magnesium sulfate, and passing through a silica gel pad, the solvent was evaporated, to afford 10 mg of the title compound as a dark brown powder.
1H-NMR (400 MHz, DMSO-D6) δ 7.11 (1H, dd, J = 3.2, 4.8 Hz) , 7.20 (1H, d, J = 16.4 Hz), 7.35 (1H, d, J = 3.2 Hz), 7.40 (1H, d, J = 8.8 Hz), 7.55 (1H, d, J = 4.8 Hz), 7.69 (1H, d, J = 16.4 Hz), 7.82 (1H, dd, J = 6.8, 8.8 Hz), 13.07 (1H, bs), 13.71 (1H, s) . Example 211
From 4-fluoro-3-[(E)-2-(thiophen-2-yl) -vinyl]-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 212-213 were obtained in accordance with the method of Example 102. Example 212
4-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 328 MH+ Example 213
4-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 368 MH+ Example 214 4-Methoxy-3-(E)-styryl-1H-indazole-5-carbonitrile
161.5 mg of 3-bromo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile
obtained by Production example 99 and 82.3 mg of (E) -2-phenylvinylboronic acid were coupled in the same manner as described in Example 194, followed by deprotection, to afford 35 mg of the title compound as brown crystals. 1H-NMR (400 MHz, CD3OD)δ4.29 (3H, s) , 7.28-7.41 (3H, m), 7.31 (1H, d, J = 8.8 Hz), 7.47 (1H, d, J = 8.8 Hz), 7.56 (1H, d, J = 16.8 Hz), 7.56-7.61 (2H, m), 7.61 (1H, d, J = 16.8 Hz). Example 215 4-Methoxy-3- (E) -styryl-2H-indazole-5-carboxylic acid amide
35 mg of 4-methoxy-3-(E)-styryl-lH-indazole-5-carbonitrile was dissolved in a mixed solvent 2 mL concentrated sulfuric acid/ 1 mL water, and stirred at 110°C for 1 hour and 15 minutes. After cooling the reaction mixture on ice, water was slowly poured under ice cooling, and the precipitated crystals were collected by filtration, andwashed with water. After drying under reduced pressure, 22 mg of the title compound was obtained as brown crude crystals.
1H-NMR (400 MHz, CD3OD)δ4.01 (3H, s), 7.20-7.78 (7H, m) , 7.33 (1H, d, J = 8.8 Hz), 7.88 (1H, d, J = 8.8 Hz). Example 216 4-methoxy~3-(E)-styryl-lH-indazole-5-carboxylic acid
22 mg of 4-methoxy-3-(E)-styryl-1H-indazole-5-carboxylic acid amide was added to 3 mL of 4N lithium hydroxide aqueous solution, and stirred at 110°C for 4 hours. After cooling on ice, the reaction solution was neutralized with 2N hydrochloric
acid under ice cooling, and the precipitated crystals were collected by filtration and washed with water. This was then dried under reduced pressure, to afford 20 mg of the title compound as brown crude crystals.
1H-NMR (400 MHz, CD3OD ) δ 4.0 (3H, s) , 7.22-7.75 (7H, m) , 7.28 (1H, d, J = 8.8 Hz), 7.88 (1H, d, J = 8.8 Hz). MS (ESI) m/z 293 (M-H)" Example 217
From 4-methoxy-3-(E)-styryl-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 218-222 were obtained in accordance with the method of Example 102. Example 218
4-Methoxy-3-styryl-1H-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-metylpropyl]-amide MS (ESI) m/z 380 MH+ Example 219
4-Methoxy-3-styryl-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 334 MH+ Example 220
4-Methoxy-3-styryl-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 374 MH+ Example 221
4-Methoxy-3-styryl-lH-indazole-5-carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 414 MH+ Example 222
4-Methoxy-3-styryl-lH-indazole-5-carboxylic acid [(15)-1-carbamoylethyl]-amide MS (ESI) m/z 365 MH+ Example 223
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carbonitrile
180.3 mg of 3-bromo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 99 and 154 mg of 2-[(E)-2-(3-fluorophenyl)-vinyl]-4,4,5,5-tetramethyl-[1, 3, 2]dioxaborolane obtained by Production example 137 were coupled in accordance with the method of Example 194, followed by deprotection, to afford 30 . 8 mg of the title compound as brown crystals.
1H-NMR (400 MHz, CD3OD) δ 4.30 (3H, s) , 6.99-7.51 (4H, m) , 7.31 (1H, d, J = 8.8 Hz), 7.48 (1H, d, J = 8.8 Hz), 7.57 (1H, d, J = 16.4 Hz), 7.61 (1H, d, J = 16.4 Hz). Example 224
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid amide
From 30.8 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carbonitrile, 22 mg of the title compound was obtained as brown crude crystals in accordance with the method of Example 215. 1H-NMR (400 MHz, CD3OD) δ 4.00 (3H, s), 7.00-7.05 (1H, m) ,
7.21-7.43 (3H, m), 7.33 (1H, d, J = 8.8 Hz), 7.57 (1H, d, J = 16.4 Hz) , 7.64 (1H, d, J = 16.4 Hz) , 7.88 (1H, d, J= 8.8 Hz). Example 225
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
From 22 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-4-methoxy-2H-indazole-5-carboxylic acid amide, 19 mg of the title compound was obtained as brown crude crystal in accordance with the method of Example 216.
1H-NMR (400 MHz, CD3OD) δ 4.05 (3H, s) , 6.99-7.04 (1H, m) , 7.22-7.41 (3H, m), 7.55 (1H, d, J = 16.4 Hz), 7.69 (1H, d, J = 16.4 Hz), 7.77 (1H, d, J = 8.6 Hz). MS (ESI) m/z 311 (M-H)~ Example 226
From 3-[(E)-2-(3-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 227-231 were obtained in accordance with the method of Example 102. Example 227
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI) m/z 398 MH+ Example 228
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 352 MH+
Example 229
3-[ (E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 392 MH+
Example 230
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 432 MH+
Example 231
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-1-carbamoylethyl]-amide
MS (ESI) m/z 383 MH+
Example 232
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carbonitrile
From 420 mg of 3-bromo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 99 and 241 mg of (E)-2-(4-fluorophenyl)vinylboronic acid, 66.2 mg of the title compound was obtained as pale yellow crystals in accordance with the method of Example 214.
1H-NMR (400 MHz, DMSO-D6) δ 4.23 (3H, s), 7.24 (2H, t, J = 8.8 Hz), 7.37 (1H, d, J = 8.6 Hz), 7.4 7 (1H, d, J = 16.4 Hz), 7.55 (1H, d, J = 8.6 Hz), 7.57 (1H, d, J = 16.4 Hz), 7.72 (2H, dd, J = 5.8, 8.8 Hz), 13.68 (1H, s) . Example 233 3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid amide
From 66.2 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carbonitrile, 61.1 mg of the title compound was obtained as pale yellow crude crystals in accordance with the method of Example 215.
1H-NMR (400 MHz, CD3OD) δ 4.00 (3H, s), 7.08-7.15 (2H, m) , 7.32 (1H, d, J = 8.6 Hz), 7.53 (1H, d, J = 16.4 Hz), 7.58 (1H, d, J = 16.4 Hz), 7.58-7.68 (2H, m), 7.87 (1H, d, J = 8.6 Hz). Example 234
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
From 61.1 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid amide, 85.2 mg of the title compound was obtained as pale yellow crude crystal in accordance with the method of Example 216.
1H-NMR (400 MHz, CD3OD) δ 4.04 (3H, s) , 7.10-7.16 (2H, m) , 7.24 (1H, d, J = 8.6 Hz), 7.55 (1H, d, J = 16.8 Hz), 7.61 (1H, d, J = 16.8 Hz), 7.60-7.65 (2H, m), 7.79 (1H, d, J = 8.6 Hz). MS (ESI) m/z 311 (M-H)~ Example 235
From 3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 236-241 were obtained in accordance with the method of Example 102. Example 236 3- [ {E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide 1H-NMR (400 MHz, CD3OD) δ 1.06 (3H, d, J = 6.6 Hz), 1.08 (3H, d, J = 6.6 Hz) , 2.02-2.12 (1H, m) , 3.7 3 (1H, dd, J = 4.4, 11.2 Hz), 3.78 (1H, dd, J = 5.2, 11.2 Hz), 3.97-4.03 (1H, m) , 4.02 (3H, s), 7.11-7.16 (2H, m ), 7.34 ( 1H, d, J = 8.8 Hz), 7.54 (1H, d, J = 16.6 Hz), 7.59 (1H, d, J= 16.6 Hz), 7.63-7.66 (2H, m) , 7.84 (1H, d, J = 8.8 Hz) . MS (ESI) m/z 398 MH+ Example 237
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 352 MH+ Example 238
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
1H-NMR (400 MHz, CD3OD) δ 3.89 (3H, s), 4.64 (2H, s) , 6.36 (1H, dd, J=0.8, 3.2 Hz), 6.39 (1H, dd, J=1.8, 3.2 Hz), 7.09-7.16 (2H, m) , 7.32 (1H, d, J = 8.8 Hz), 7.47 (1H, dd, J = 0.8, 1.8 Hz), 7.53 (1H, d, J=16.4Hz), 7.58 (1H, d, J=16.4Hz), 7.59-7.66 (2H, m), 7.78 (1H, d, J = 8.8 Hz). MS (ESI) m/z 392 MH+ Example 239
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-lH-indazole-5-carboxylic acid [(15)-2-hydroxy-l-phenylethyl]-amide 1H-NMR (400 MHz, CD3OD) δ 3.88 (1H, dd, J =6.6, 11.4 Hz), 3.94 (1H, dd, J= 4.8, 11.4 Hz), 3.97 (3H, s), 5.22-5.25 (1H, m),
7.14 (2H, t, J = 8.8 Hz) , 7.2 6-7.39 (4H, m) , 7.44-7.48 (2H, m) , 7.55 (1H, d, J = 16.4 Hz) , 7.60 (1H, d, J = 16.4 Hz) , 7.63-7.67 (2H, m), 7.81 (1H, d, J = 8.8 Hz). MS (ESI) m/z 432 MH+ Example 24 0
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-2H-indazole-5-carboxylic acid [(1S)-1-carbamoylethyl]-amide 1H-NMR (400 MHz, CD3OD) δ 1.52 (3H, d, J = 7.0 Hz), 4.01 (3H, s), 4.69 (1H, q, J = 7.0 Hz) , 7.12-7.16 (2H, m), 7.35 (1H, d, J = 8.6 Hz), 7.55 (1H, d, J = 16.2 Hz), 7.60 (1H, d, J = 16.2 Hz), 7.64-7.67 (2H, m), 7.89 (1H, d, J = 8.6 Hz). MS (ESI) m/z 383 MH+ Example 241
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (tetrahydrofuran-2-ylmethyl)-amide MS (ESI) m/z 396 MH+ Example 242
4-Methoxy-3- [ (E) -2- (p-tolyl) -vinyl] -lH-indazole-5-carbonitrile
From 160 mg of 3-bromo-4-methoxy-l-trityl-1H-indazole-5-carbonitrile obtained by Production example 99 and 89 mg of (E)-2-(4-methylphenyl)vinylboronic acid, 46.7 mg of the title compound was obtained as colorless crystals in accordance with the method of Example 214.
1H-NMR (400 MHz, CD3OD) δ 2.23 (3H, s ) , 4.14 (3H, s) , 6.09-7.42 (5H, m), 7.06-7.09 (2H, m), 7.15 (1H, d, J = 8.4 Hz).
Example 24 3
4-Methoxy-3-[ (E) -2-(p-tolyl)-vinyl]-1H-indazole-5-
carboxylic acid
By treating 46.7 mg of 4-methoxy-3-[(E)-2-(p-tolyl)-vinyl]-lH-indazole-5-carbonitrile in the method according to Example 216, 30.8 mg of the title compound was obtained as colorless crude crystals.
1H-NMR (400 MHz, CD3OD) δ 2.37 (3H, s), 4.04 (3H, s) , 7.21 (2H, d, J = 8.2 Hz), 7.26 (2H, d, J = 8.8 Hz), 7.49 (2H, d, J = 8.2 Hz), 7.54 (1H, d, J = 16.4 Hz), 7.61 (1H, d, J = 16.4 Hz), 7.83 (1H, d, J = 8.8 Hz). MS (ESI) m/z 309 MH+ Example 24 4
From 4-methoxy-3-[(E)-2-(p-tolyl)-vinyl]-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 245-249 were obtained in accordance with the method of Example 102.
Example 245
4-Methoxy-3-[(E)-2-(p-tolyl)-vinyl]-lH-indazole-5-carboxylic acid [(15)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI) m/z 394 MH+ Example 24 6
4-Methoxy-3-[(E) -2-(p-tolyl)-vinyl]-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 348 MH+ Example 247
4-Methoxy-3-[(E)-2-(p-tolyl)-vinyl]-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 388 MH+
Example 248
4-Methoxy-3-[(E)-2-(p-tolyl)-vinyl]-lH-indazole-5-
carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 428 MH+
Example 24 9
4-Methoxy-3-[(E)-2-(p-tolyl)-vinyl]-1H-indazole-5-
carboxylic acid [(1S)-1-carbamoylethyl]-amide
MS (ESI) m/z 379 MH+
Example 250
3-[(E)-2- (4-Chlorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid
160 mg of 3-bromo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 99 and 100.3 mg of (E)-2-(4-chlorophenyl)vinylboronic acid were allowed to react in accordance with the method of Example 214, and then alkaline hydrolyzedin accordance with the method of Example 216, to afford 64.8 mg of the title compound as orange crude crystals. MS (ESI) m/z 327 (M-H)~ Example 251
From 3-f(E)-2-(4-chlorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 252-256 were obtained in accordance with the method of Example 102-h.
Example 252
3-[(E)-2-(4-Chlorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide
MS (ESI) m/z 415 MH+
Example 253
3- [ (E) -2-(4-Chlorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 369 MH+
Example 254
3- [ (E) -2-(4-Chlorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 409 MH+
Example 255
3- [ (E) -2-(4-Chlorophenyl)-vinyl]-4-methoxy-lH-indazole-5~
carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 449 MH+
Example 256
3-[{E)-2-(4-Chlorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-1-carbamoylethyl]-amide
MS (ESI) m/z 400 MH+
Example 257
3- [ {E) -2-(4-Aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid
180 mg of 3-bromo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 99 and 185.5 mg of {4-[(E)-2-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-
vinyl]-phenyl}-carbamic acid tert-butyl ester obtained by
Production example 138 were coupled in accordance with the method
of Example 214, followed by alkaline hydrolysis in accordance
with the method of Example 216, to afford 30 mg of the title
compound as brown crude crystals.
1H-NMR (400 MHz, CD3OD) δ 4.03 (3H, s), 6.72 (2H, d, J = 8.6
Hz), 7.19 (1H, d, J = 8.6 Hz), 7.36 (2H, d, J = 8.6 Hz), 7.41
(1H, d, J = 16.6 Hz), 7.46 (1H, d, J = 16.6 Hz), 7.73 (1H, d,
J = 8.6 Hz) .
MS (ESI) m/z 308 (M-H)~
Example 258
From 3-[(E)-2-(4-aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 259-263 were obtained in accordance with the method of Example 10. Example 259
3- [ (E) -2-(4-Aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI) m/z 395 MH+ Example 2 60
3- [ (E) -2-(4-Aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 349 MH+ Example 2 61
3- [ (E) -2-(4-Aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 389 MH+
Example 2 62
3-[(E)-2-(4-Aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 429 MH+
Example 2 63
3-[(E)-2-(4-Aminophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(1S)-1-carbamoylethyl]-amide
MS (ESI) m/z 380 MH+
Example 2 64
3-[(E)-2-(2-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carbonitrile
208 mg of 3-bromo-4~methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 99 and 177 mg of 2-[(E)-2-(2-fluorophenyl)-vinyl]-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane obtained by Production example 139 were coupled in accordance with the method of Example 214, followed by deprotection, to afford 31mg of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 4.35 (3H, s), 7.44 (1H, d, J = 8.8 Hz) , 7.05-7.29 (3H, m) , 7.19 (1H, d, J = 8.8 Hz) , 7.60-7.65 (1H, m), 7.65 (1H, d, J = 16.6 Hz), 7.78 (1H, d, J = 16.6 Hz). Example 265
3-[(E)-2-(2-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
From 31 mg of 3-[(E)-2-(2-fluorophenyl)-vinyl]-4-
methoxy-lH-indazole-5-carbonitrile, 20 mg of the title compound was obtained as yellow crude crystals in accordance with the method of Example 216.
1H-NMR (400 MHz, CD3OD) δ 4.03 (3H, s), 7.12-7.17 (1H, m), 7.20-7.24 (1H, m) , 7.28-7.34 (1H, m) , 7.2 9 (1H, d, J = 8.8 Hz) , 7.70-7.75 (1H, m), 7.73 (1H, d, J = 15.6 Hz), 7.77 (1H, d, J = 15.6 Hz), 7.88 (1H, d, J = 8.8 Hz). Example 2 66
From 3-[(E)-2-(2-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 267-271 were obtained in accordance with the method of Example 102. Example 2 67
3-[(E)-2-(2-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI) m/z 398 MH+ Example 2 68
3-[(E)-2-(2-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 352 MH+ Example 269
3-[(E)-2-(2-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 392 MH+ Example 270 3-[(E)-2-(2-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 432 MH+
Example 271
3- [ (E) -2- (2-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-1-carbamoylethyl]-amide
MS (ESI) m/z 383 MH+
Production example 272
4-Methoxy-lH-indazole-5-carboxylic acid amide
By treating 4 . 7 g of 4-methoxy-1H-indazole-5-carbonitrile obtained by Production example 97 in the same method as described in Example 215, 15 g of a crude product of the title compound was obtained as brown crystals.
1H-NMR (400 MHz, DMSO-D6) δ4 .28 (3H, s), 7.14 (1H, d, J = 8.8 Hz), 7.44 (1H, bs), 7.60 (1H, bs), 7.80 (1H, d, J = 8.8 Hz), 8.43 (1H, s). Production example 273 4-Methoxy-lH-indazole-5-carboxylic acid
By treating 15 g of 4-methoxy-lH-indazole-5-carboxylic acid amide in the manner as described in Example 216, 4.5 g of the title compound was obtained as brown crude crystals. 1H-NMR (400 MHz, CD3OD) δ4.31 (3H, s) , 7.18 (1H, d, J = 8.8 Hz) , 7.84 (1H, d, J = 8.8 Hz), 8.39 (1H, s). Production example 274 4-Methoxy-lH-indazole-5-carboxylic acid ethyl ester
2 g of 4-methoxy-lH-indazole-5-carboxylic acid was added to a mixed solvent of 40 mL ethanol/2.2 mL concentrated sulfuric
acid, and stirred at 95°C for 11 hours. After cooling the reaction solution on ice, water was slowly added under ice cooling, and the precipitated crystals were collected by filtration and washed with water. Thereafter, the crystals were dried under reduced pressure, to afford 1. 52 g of the title compound as pale brown crude crystals.
1H-NMR (400 MHz, CDCl3) δ1.41 (3H, t, J = 7.0 Hz), 4.26 (3H, s), 4.38 (2H, q, J = 7.0 Hz), 7.12 (1H, d, J = 8.8 Hz), 7.86 (1H, d, J = 8.8 Hz), 8.30 (1H, s). Production example 275 3-Iodo-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester
To 15 mL of a solution of 1. 33 g of 4-methoxy-1H-indazole-5-carboxylic acid ethyl ester in N,N-dimethylf ormamide were added 1.95 g of iodine and 0.85 g of potassium hydroxide, stirred at room temperature for 1 hour and 40 minutes, added with another 0.8 g of iodine, and stirred for 3 hours and 20 minutes. Thereafter, 10% sodium hydrogensulfite aqueous solution was added, extracted twice with ethyl acetate, and the organic layer was washed once with saturated brine, dried over magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography, to afford 1.68 g of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ1.43 (3H, t, J = 7.2 Hz), 4.07 (3H, s), 4.42 (2H, q, J = 7.2 Hz), 7.24 (1H, d, J = 9.0 Hz), 7.92 (1H, d, J = 9.0 Hz), 10.36 (1H, bs). Production example 276
3-Iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester
From 1.68 g of 3-iodo-4-methoxy-1H-indazole-5-carboxylic acid ethyl ester, 3.21 g of the title compound was obtained as colorless crude crystals in accordance with the method of Production example 94.
1H-NMR (400 MHz, CDC13)δ 1.37 (3H, t, J = 7.2 Hz), 4.05 (3H, s), 4.35 (2H, q, J=7.2Hz), 6.14 (1H, d, J=9.0Hz), 7.14-7.17 (5H, m) , 7.25-7.30 (10H, m), 7.45 (1H, d, J = 9.0 Hz). Production example 277
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester
From 560 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester and 3-acetylstyrene, 150 mg of the title compound was obtained as pale yellow needle crystals in accordance with the method of Production example 181. 1H-NMR (400 MHz, CDCl3) δ 1.38 (3H, t, J = 7.5 Hz), 2.63 (3H, s), 4.05 (3H, s), 4.37 (2H, q, J = 7.5 Hz), 6.14 (1H, d, J = 8.8 Hz), 7.17-7.25 (6H, m), 7.25-7.32 (9H, m), 7.45 (1H, d, J = 8.8 Hz), 7.45 (1H, t, J=7.7Hz), 7.53 (1H, d, J = 16.3 Hz) , 7.69 (1H, d, J = 16.3 Hz), 7.74 (1H, d, J = 7.7 Hz), 7.84 (1H, d, J = 7.7 Hz), 8.08 (1H, bs). Example 278
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester
By treating 150 mg of 3-[[E)-2-(3-acetylphenyl)-vinyl]-4-
methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 16, 70 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 1.44 (3H, t, J = 7.5 Hz), 2.65 (3H, s), 4.05 (3H, s), 4.43 (2H, q, J = 7.5 Hz), 7.23 (1H, d, J =
8.6 Hz), 7.49 (1H, t, J = 7.7 Hz), 7.71 (1H, d, J = 16.8 Hz),
7.7 4 (1H, d, J = 16.8 Hz) , 7.8 0 (1H, bd, J = 7.7 Hz) , 7.8 9 (1H,
bd, J = 7.7 Hz), 7.91 (1H, d, J = 8.6 Hz), 8.16 (1H, bs).
Example 27 9
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid
By treating 70 mg of 3-[(E)-2-(3-acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 144, 65 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 2.63 (3H, s), 3.96 (3H, s) , 7.30 (1H, d, J = 8.6 Hz), 7.56 (1H, t, J = 7.7 Hz), 7.63 (1H, d, J = 16.4 Hz) , 7.68 (1H, d, J = 16.4 Hz) , 7.7 3 (1H, d, J = 8.6 Hz) , 7.87 (1H, bd, J = 7.7 Hz) , 7.93 (1H, bd, J = 7.7 Hz) , 8.15 (1H, bs), 12.60-12.75 (1H, bs), 13.49 (1H, bs) . Example 28 0
From 3-[(E)-2-(3-acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Example 281-287 were obtained in accordance with the method of Example 102. Example 281
3- [ (E) -2- (3-Acetylphenyl) -vinyl] -4-methoxy-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 376 MH+
Example 282
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 416 MH+
Example 283
3- [ (E) -2- (3-Acetylphenyl) -vinyl] -4-methoxy-lH-indazole-5-
carboxylic acid [(1S)-l-hydroxymethyl-2-methyl-propyl]-amide
MS (ESI) m/z 422 MH+
Example 284
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 432 MH+
Example 285
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (l-carbamoyl-2-phenyl-ethyl)-amide
MS (ESI) m/z 483 MH+
Example 28 6
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (l-carbamoyl-2-hydroxy-ethyl)-amide
MS (ESI) m/z 423 MH+
Example 287
3-[(E)-2-(3-Acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 407 MH+
Production example 288
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-l-trityl-lH-
indazole-5-carboxylic acid ethyl ester
By treating 560 mg of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 4-acetylstyrene in the similar method as described in Production example 181, 100 mg of the title compound was obtained as pale yellow needle crystals.
1H-NMR (400 MHz, CDCl3) δ 1.38 (3H, t, J = 7.5 Hz), 2.61 (3H, s), 4.06 (3H, s), 4.38 (2H, q, J = 7.5 Hz), 6.14 (1H, d, J = 9.3 Hz), 7.18-7.25 (6H, m), 7.25-7.32 (9H, m), 7.45 (1H, d, J = 9.3 Hz), 7.49 (1H, d, J = 16.4 Hz) , 7.60 (2H, d, J=8.3Hz), 7.75 (1H, d, J = 16.4 Hz), 7.84 (2H, d, J = 8.3 Hz). Example 28 9
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl
By treating 100 mg of 3-[(E)-2-(4-acetylphenyl)-vinyl]-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 16, 50 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 1.45 (3H, t, J = 7.2 Hz), 2.63 (3H, s), 4.07 (3H, s), 4.43 (2H, q, J = 7.2 Hz), 7.24 (1H, d, J = 9.1 Hz), 7.67 (2H, d, J = 8.5 Hz), 7.70 (1H, d, J = 16.2 Hz), 7.7 9 (1H, d, J = 16.2 Hz) , 7.92 (1H, bd, J = 9.1 Hz) , 7.99 (2H, d, J = 8.5 Hz).
Example 2 90
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid
By treating 50 mg of 3-[(E)-2-(4-acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 144, 45 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 2.58 (3H, s) , 3.96 (3H, s) , 7.30 (1H, d, J = 8.8 Hz), 7.62 (1H, d, J = 16.4 Hz), 7.73 (1H, d, J = 16.4 Hz), 7.74 (1H, d, J = 8.8 Hz), 7.78 (2H, d, J = 8.5 Hz) , 7.97 (2H, d, J = 8.5 Hz) , 12.60-12.75 (1H, bs) , 13.54 (1H, bs) . Example 291
From 3-[(E)-2-(4-acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 292-298 were obtained in accordance with the method of Example 102. Example 292
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-ca rboxylic acid cyclopropylamide MS (ESI) m/z 376 MH+ Example 293
3-[{E)-2-4-Acetylphenyl]-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 416 MH+ Example 2 94
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(1S)-l-hydroxymethyl-2-methyl-propyl]-amide
MS (ESI) m/z 422 MH+
Example 2 95
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 432 MH+
Example 2 96
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (l-carbamoyl-2-phenyl-ethyl)-amide
MS (ESI) m/z 483 MH+
Example 297
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (l-carbamoyl-2-hydroxy-ethyl)-amide
MS (ESI) m/z 423 MH+
Example 298
3-[(E)-2-(4-Acetylphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 407 MH+
Production example 299
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl] -1-trityl-1H-
indazole-5-carboxylic acid ethyl ester
By treating 560 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 160 ul of 4-vinylpyridine in the similar method as described in Production example 181, 300 mg of the title
compound was obtained as a pale yellow oil.
1H-NMR (400 MHz, CDCl3) δ 1.38 (3H, t, J = 7.5 Hz), 4.04 (3H, s), 4.38 (2H, q, J=7.5Hz), 6.15 (1H, d, J=8.8Hz), 7.17-7.23 (6H, m), 7.26-7.32 (9H, m), 7.17 (2H, d, J = 6.0 Hz), 7.18 (1H, d, J = 16.4 Hz), 7.4 6 (1H, d, J = 8.8 Hz) , 7.82 (1H, d, J = 16.4 Hz), 8.57 (2H, d, J = 6.0 Hz). Example 300
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester
By treating 300 mg of 4-methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-l-trityl-lH-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 16, 150 mg of the title compound was obtained as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 1.36 (3H, t, J =7.5 Hz), 3.97 (3H, s), 4.33 (2H, q, J = 7.5 Hz), 7.37 (1H, d, J = 8.7 Hz), 7.55 (1H, d, J = 16.4 Hz), 7.63 (2H, d, J = 5.7 Hz), 7.74 (1H, d, J = 8.7 Hz), 7.84 (1H, d, J = 16.4 Hz), 8.58 (2H, d, J = 5.7 Hz) .
Example 301
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-lH-indazole-5-carboxylic acid
150 mg of 4-methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester was dissolved in a mixed solvent of 3 mL tetrahydrofuran/1 mL methanol, added with 0.5 mL of 5N sodium hydroxide aqueous solution, and heated at 50°C for 4 hours. The reaction solution was neutralized with acetic
acid, and the solvent distilled off under reduced pressure, to give 500 mg of the crudely produced title compound as a mixture with sodium acetate.
1H-NMR (400MHz, DMSO-D6) δ 4.01 (3H, s), 7.13 (1H, d, J =8.7 Hz), 7.49 (1H, d, J = 16.4 Hz), 7.54 (1H, d, J= 8.7 Hz), 7.58 (2H, d, J = 6.3 Hz), 7.87 (1H, d, J = 16.4 Hz), 8.56 (2H, d, J = 6.3 Hz). Example 302
From 4-methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 303-309 were obtained in accordance with the method of Example 102. Example 303
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 335 MH+ Example 304
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 375 MH+ Example 305
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-lH-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methyl-propyl]-amide MS (ESI) m/z 381 MH+ Example 306 4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-lH-indazole-5-

carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 391 MH+
Example 307
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-1H-indazole-5-
carboxylic acid (l-carbamoyl-2-phenyl-ethyl)-amide
MS (ESI) m/z 442 MH+
Example 308
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-1H-indazole-5-
carboxylic acid (l-carbamoyl-2-hydroxy-ethyl)-amide
MS (ESI) m/z 382 MH+
Example 309
4-Methoxy-3-[(E)-2-(pyridin-4-yl)-vinyl]-lH-indazole-5-
carboxylic acid [(1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 366 MH+
Production example 310
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1-
trityl-lH-indazole-5-carboxylic acid ethyl ester
By treating 560 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 500 mg of crudely produced 2-methoxy-5-vinylpyridine in the similar method as described in Production example 181, 70 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 1.38 (3H, t, J = 7.5 Hz), 3.96 (3H, s), 4.03 (3H, s), 4.37 (2H, q, J = 7.5 Hz), 6.11 (1H, d, J = 8.5Hz), 6.75 (1H, d, J = 8.5 Hz) , 7.15-7.24 (6H, m) , 7.24-7.36
(10H, m) , 7.44 (1H, d, J = 8.5 Hz) , 7.52 (1H, d, J = 16.3 Hz) , 7.81 (1H, dd, J = 2.2, 8.5 Hz), 8.24 (1H, d, J = 2.2 Hz). Example 311
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester
By treating 70 mg of 4-methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-l-trityl-1H-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 16, 35 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, CD3OD) δ 1.42 (3H, t, J = 7.5 Hz), 3.94 (3H, s), 4.01 (3H, s), 4.38 (2H, q, J = 7.5 Hz), 6.89 (1H, d, J = 9.0 Hz), 7.28 (1H, d, J = 8.8 Hz), 7.53 (1H, d, J = 16.6 Hz), 7.57 (1H, d, J = 16.6 Hz), 7.83 (1H, d, J = 8.8 Hz), 8.06 (1H, dd, J = 2.4, 9.0 Hz), 8.28 (1H, d, J = 2.4 Hz). Example 312
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid
By treating 35 mg of 4-methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid ethyl ester in the similar method as described in Example 144, 30 mg of the title compound was obtained as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.89 (3H, s) , 3.97 (3H, s), 6.89
(1H, d, J = 8.7 Hz), 7.28 (1H, d, J = 8.7 Hz), 7.51 (1H, d, J = 16.4 Hz), 7.54 (1H, d, J = 16.4 Hz) , 7.73 (1H, d, J = 8.7 Hz) , 8.09 (1H, dd, J = 2.2, 8.7 Hz), 8.39 (1H, d, J = 2.2 Hz).
Example 313
From 4-methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl] -lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 314-320 were obtained in accordance with the method of Example 102-h. Example 314
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 365 MH+ Example 315
4-Methoxy-3-[{E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 405 MH+ Example 316
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methyl-propyl]-amide MS (ESI) m/z 411 MH+ Example 317
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid (thiophen-2-ylmethyl)-amide MS (ESI) m/z 421 MH+ Example 318
4-Methoxy-3-[{E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid (l-carbamoyl-2-phenyl-ethyl)-amide
MS (ESI) m/z 472 MH+
Example 319
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-
indazole-5-carboxylic acid (l-carbamoyl-2-
hydroxy-ethyl)-amide
MS (ESI) m/z 412 MH+
Example 320
4-Methoxy-3-[(E)-2-(6-methoxypyridin-3-yl)-vinyl]-1H-
indazole-5-carboxylic acid [ (1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 396 MH+
Production example 321
3-Iodo-4-methoxy-lH-indazole-5-carbonitrile
From 28.4 g of 4-methoxy-1H-indazole-5-carbonitrile obtained by Production example 97, 52.8 g of the title compound was obtained as yellow crude crystal in accordance with themethod of Production example 275.
1H-NMR (400 MHz, DMSO-D6) δ4 .14 (3H, s) , 7.42 (1H, d, J = 8.6 Hz), 7.56 (1H, d, J = 8.6 Hz), 13.96 (1H, s). Production example 322 3-Iodo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile
From 52.8 g of 3-iodo-4-methoxy-lH-indazole-5-carbonitrile, 104 . 5 g of the title compound was obtained as brown crude crystal in accordance with the method of Production example 94.
1H-NMR (4 00 MHz, CDC13)δ4.31 (3H, s), 6.13 (1H, d, J= 9.0 Hz), 7.00 (1H, d, J = 9.0 Hz), 7.10-7.18 (5H, m), 7.22-7.37 (10H,
m) .
Production example 323
3-Vinyl-4-methoxy-l-trityl-1H-indazole-5-carbonitrile
By treating 1.02 g of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile in the method of Production example 123, 700 mg of the title compound was obtained as colorless needle crystals.
1H-NMR (400 MHz, CDCl3) δ 4.27 (3H, s) , 5.35 (1H, d, J = 2.0, 11.0 Hz), 6.05 (1H, d, J= 8.7 Hz), 6.08 (1H, dd, J=2.0, 17.9 Hz), 6.96 (1H, d, J = 8.7 Hz), 7.12-7.18 (6H, m) , 7.14 (1H, d, J = 11.0, 17.9 Hz), 7.25-7.34 (9H, m) . Production example 324
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole-5-carbonitrile
300 mg of 3-vinyl-4-methoxy-l-trityl-lH-indazole-5-carbonitrile and 180 µl of 3-bromopyridine were dissolved in a mixed solvent of 1 mL triethylamine/5 mL acetonitrile, added with 40 mg of tri-p-tolylphosphine and 27 mg of palladium acetate (II), and heated at 110°C for 24 hours. The reaction solution was added with silica gel, the solvent was evaporated, and the resultant residue was purified by column chromatography (hexane:ethyl acetate =7:3), to afford 120 mg of the title compound as a pale yellow oil.
1H-NMR (400 MHz, CDCl3) δ 4.35 (3H, s), 6.10 (1H, d, J = 9.0 Hz) , 7.00 (1H, d, J = 9.0 Hz) , 7.16-7.22 (6H, m) , 7.2 6-7.34 (10H, m) , 7.41 (1H, d, J = 16.1 Hz) , 7.60 (1H, d, J = 16.1 Hz) , 7.83
(1H, dt, J=2.2, 8.3 Hz), 8.50 (1H, dd, J=2.2, 5.3 Hz), 8.72
(1H, d, J = 2.2 Hz). Example 325
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carbonitrile
By treating 120 mg of 4-methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-l-trityl-1H-indazole-5-carbonitrile in the similar method as described in Example 16, 7 6 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 4.24 (3H, s) , 7.40 (1H, d, J =8.5 Hz) , 7.4 5 (1H, dd, J = 4.8, 8.2 Hz) , 7.57 (1H, d, J = 8.5 Hz) , 7.59 (1H, d, J= 16.6 Hz), 7.66 (1H, d, J= 16.6 Hz), 8.14 (1H, dt, J = 1.9, 8.2 Hz) , 8.51 (1H, dd, J = 1.9, 4.8 Hz) , 8.84 (1H, d, J = 1.9 Hz), 13.77 (1H, bs). Example 32 6
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid
70 mg of 4-methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole-5-carbonitrile was alkaline hydrolyzed in the similar method as described in Example 243, to afford 42 mg of the title compound as a pale yellow powder.
1H-NMR (400 MHz, DMSO-D6) δ 3.97 (3H, s) , 7.32 (1H, d, J =8.6 Hz), 7.44 (1H, dd, J = 4.5, 7.7 Hz), 7.59 (1H, d, J = 16.4 Hz) , 7.70 (1H, d, J = 16.4 Hz), 7.75 (1H, d, J = 8.6 Hz), 8.12 (1H, dt, J=1.8, 7.7Hz), 8.50 (1H, dd, J=1.8, 4.5 Hz), 8.72 (1H, d, J = 1.8 Hz), 13.53 (1H, bs).
Example 327
From 4-methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 328-334 were obtained in accordance with the method of Example 102. Example 328
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 375 MH+ Example 329
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid (tetrahydro-furan-2-ylmethyl)-amide MS (ESI) m/z 379 MH+ Example 330
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid (2-acetylamino-ethyl)-amide MS (ESI) m/z 380 MH+ Example 331
4-Methoxy-3-[{E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid [ (1S)-l-hydroxymethyl-2-methyl-propyl]-amide MS (ESI) m/z 381 MH+ Example 332
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid [(1S)-2-hydroxy-l-phenyl-ethyl]-amide MS (ESI) m/z 415 MH+ Example 333
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 391 MH+
Example 334
4-Methoxy-3- [ (E) -2- (pyridin-3-yl)-vinyl]-1H-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 335 MH+
Example 335
4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-
carbonitrile
2-Vinylthiophene and 500 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 322 were reacted in accordance with Example 100, to afford 110 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, DMS0-D6) δ 4.21 (3H, s) , 7.11 (1H, dd, J =3.7, 5.3 Hz), 7.27 (1H, d, J = 16.3 Hz), 7.34 (1H, bd, J = 3.7 Hz), 7.38 (1H, d, J = 8.5 Hz), 7.54 (1H, bd, J = 5.3 Hz), 7.55 (1H, d, J = 8.5 Hz), 7.72 (1H, d, J = 16.3 Hz). Example 336
4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid
110 mg of 4-methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carbonitrile was alkaline hydrolyzed in the similar method as described in Example 243, to afford 40 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.94 (3H, s), 7.11 (1H, dd, J =3.6,
5.1 Hz), 7.31 (1H, d, J = 5.1 Hz), 7.32 (1H, d, J = 8.6 Hz), 7.35 (1H, d, J = 16.1 Hz) , 7.52 (1H, bd, J = 3.6 Hz) , 7.72 (1H, d, J = 16.1 Hz), 7.75 (1H, d, J = 8.6 Hz). Example 337
From 4-methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 338-342 were obtained in accordance with the method of Example 102. Example 338
4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 340 MH+ Example 339
4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 380 MH+ Example 34 0
4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methyl-propyl]-amide MS (ESI) m/z 386 MH+ Example 341
4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazole-5-carboxylic acid [(15)-2-hydroxy-l-phenyl-ethyl]-amide MS (ESI) m/z 420 MH+ Example 342 4-Methoxy-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 396 MH+
Example 343
4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-1H-indazole-5-
carbonitrile
From 2-vinylpyridine and 500 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 322, 110 mg of the title compound was obtained as a colorless powder in accordance with Example 100. 1H-NMR (400 MHz, DMSO-D6) δ 4.23 (3H, s), 7.30 (1H, ddd, J = 1.1, 4.6, 7.6 Hz), 7.41 (1H, d, J = 8.8 Hz), 7.57 (1H, d, J = 8.8 Hz), 7.61 (1H, d, J = 7.6 Hz), 7.62 (1H, d, J = 16.0 Hz), 7.82 (1H, dt, J = 1.8, 7.6 Hz), 8.05 (1H, d, J = 16.0 Hz) , 8.64 (1H, ddd, J = 1.1, 1.8, 4.6 Hz), 13.73-13.85 (1H, bs). Example 344
4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid
110 mg of 4-methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-1H-indazole-5-carbonitrile was alkaline hydrolyzed in the similar method as described in Example 243, to afford 40 mg of the title compound as a pale yellow powder.
1H-NMR (400MHz, DMSO-D6) δ 3.95 (3H, s), 7.27 (1H, dd, J =5.0, 7.7 Hz), 7.30 (1H, d, J = 8.5 Hz), 7.56 (1H, d, J = 7.7 Hz), 7.58 (1H, d, J = 16.1 Hz), 7.73 (1H, d, J = 8.5 Hz), 7.79 (1H, dt, J = 1.7, 7.7 Hz) , 8.06 (1H, d, J = 16.1 Hz) , 8.61 (1H, bd, J = 5.0 Hz), 13.53 (1H, bs).
Example 345
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-5-
carboxylic acid ethyl ester
To a solution of 5.05 g of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 in 70 mL of 1,2-dimethoxyethane were successively added 1.57 g of (E)-2-(4-fluorophenyl)-vinylboronic acid, a solution containing 2.16 g of sodium hydrogen carbonate in 34 mL of water and 4 96.9 mg of tetrakis(triphenylphosphine)palladium(0), and stirred under nitrogen atmosphere at 110 °C for 21 hours . The reaction solution was cooled to room temperature, poured slowly with saturated aqueous ammonium chloride, extracted with ethyl acetate twice, and the resultant organic layer was washed each once with water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, to obtain a crude coupling product. The obtained crude product was dissolved in 85 mL of dichloromethane, added with 21.5 mL of a boron tribromide 1M solution in dichloromethane under ice cooling, and stirred under nitrogen atmosphere at room temperature for 19 hours. Thereafter, saturated aqueous ammonium chloride was poured slowly, and extracted twice with a mixed solvent of ethyl acetate:tetrahydrofuran=l:1, and the resultant organic layer was washed once with saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the obtained crude product was purified and separated by silica gel column
chromatography, to afford 608 mg of the title compound as orange crystals.
1H-NMR (400 MHz, DMSO-D6) δ 1.37 (3H, t, J =6.8 Hz), 4.40 (2H, q, J = 6.8 Hz), 7.04 (1H, d, J = 8.8 Hz), 7.25 (2H, t, J = 8.8 Hz) , 7.58 (1H, d, J = 16.8 Hz) , 7. 64 (1H, d, J = 16.8 Hz) , 7. 64-7.72 (3H, m), 12.21 (1H, s), 13.47 (1H, s). Production example 34 6
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-1H-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester
To a solution of 553 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-5-carboxylic acid ethyl ester in 15 mL of tetrahydrofuran were added 257.5 mg of di-tert-butyl dicarbonate and 41.5 mg of 4-(dimethylamino)pyridine, and stirred for 1 hour and 15 minutes under ice cooling. Then the reaction solution was added with water, extracted twice with diethyl ether, and the resultant organic layer was washed each once with water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography, to afford 431.2 mg of the title compound as colorless crystals. 1H-NMR (400MHz, CDCl3)δ1.45 (3H, t, J =7.2 Hz), 1.74 (9H, s), 4.4 6 (2H, q, J = 7.2 Hz) , 7.05-7.11 (2H, m) , 7.58-7.64 (2H, m) , 7.61 (1H, d, J = 9.0 Hz), 7.65 (1H, d, J = 16.2 Hz), 7.87 (1H, d, J = 16.2 Hz), 7.94 (1H, bs), 12.15 (1H, s). Production example 347 3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-[2-(tetrahydropyran-2-
yloxy)-ethoxy]-1H-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester
To a solution of 200 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-hydroxy-1H-indazole-l,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester in 13 mL of N,N-dimethylformamide were added 196.5 mg of 2-(2-bromoethoxy)-tetrahydropyran and 306.3 mg of cesium carbonate, stirred for 1 hour and 30 minutes at room temperature, stirred at 50°C for 17 hours, added with 196.5 mg of 2-(2-bromoethoxy)-tetrahydropyran and 306.3 mg of cesium carbonate, stirred for 3 hours at room temperature and for 2 hours at 50°C, added with 554 mg of
2- (2-bromoethoxy) -tetrahydropyran, and further stirred at 50°C for 4 hours. Then, the reaction solution was added saturated aqueous ammonium chloride, extracted twice with ethyl acetate, and the resultant organic layer was washed once with saturated brine and dried over magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography, to afford 147 mg of the title compound as a colorless oil.
1H-NMR (4 00 MHz, CDCl3) δ1. 36-1. 79 (6H, m) , 1.4 4 (3H, t, J = 7.2 Hz) , 1.75 (9H, s) , 3.4 0-3.5 6 (1H, m) , 3.74-3.84 (1H, m) , 3.85-3.94 (1H, m), 4.09-4.16 (1H, m), 4.28-4.47 (2H, m), 4.43 (2H, q, J = 7.2 Hz) , 4.57-4.61 (1H, m) , 7.02-7.10 (2H, m) , 7.60-7.66 (2H, m) , 7.70 (1H, d, J = 16.4 Hz), 7.81 (1H, d, J = 16.4 Hz), 7.86 (1H, d, J = 8.8 Hz), 7.99 (1H, d, J = 8.8 Hz).
Production example 348
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-1H-
indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl
ester
To a solution of 147 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl] -4- [2- (tetrahydropyran-2-yloxy) -ethoxy] -2H-indazole-1, 5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester in 1.5 mL of tetrahydrofuran was added 1.5 mL of 2N hydrochloric acid, and stirred at room temperature for 19 hours. Thereafter, the reaction solution was added with water and extracted twice ethyl acetate, and the resultant organic layer was washed once with saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography, to afford 87.1 mg of the title compound as a colorless oil.
1H-NMR (4 00 MHz, CDCl3)δ1.4 3 (3H, t, J=6.8Hz), 1.75 (9H, s), 3.42-3.45 (1H, m), 3.96-4.00 (2H, m), 4.24-4.26 (2H, m), 4.43 (2H, q, J = 6.8 Hz), 7.05-7.12 (2H, m), 7.57 (1H, d, J = 16.4 Hz), 7.57-7.63 (2H, m), 7.80 (1H, d, J = 16.2 Hz), 7.93 (1H, d, J = 8.8 Hz), 8.08 (1H, d, J = 8.8 Hz). Example 34 9
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-1H-indazole-5-carboxylic acid ethyl ester
By treating 87.lmgof 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-lH-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester in the similar method as
described in Example 16, 31. 3 mg of the title compound was obtained
as a colorless oil.
1H-NMR (400 MHz, CDC13)δ1.42 (3H, t, J = 6.8 Hz), 3.99 (2H, t,
J= 4.0Hz), 4.29 (2H, t, J = 4.0 Hz), 4.41 (2H, q, J= 6.8 Hz),
7.01-7.10 (2H, m) , 7.22 (1H, d, J = 8.8 Hz) , 7.4 8-7.58 (2H, m) ,
7.53 (1H, d, J = 16.4 Hz) , 7.60 (1H, d, J = 16.4 Hz) , 7.91 (1H,
d, J = 8.8 Hz).
MS (ESI) m/z 371 MH+
Example 350
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-1H-
indazole-5-carboxylic acid
To a solution of 31.3 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-lH-indazole-5-carboxylic acid ethyl ester obtained by Example 349-j in0.7 mL of tetrahydrofuran were added 0.3 mL of ethanol and 0.2 mL of 5N sodium hydroxide aqueous solution, and stirred at 70 °C for 1 hour and 20 minutes. After cooling on ice, the solution was neutralized with 2N hydrochloric acid under ice cooling, and the precipitated crystals were collected by filtration, and dried under reduced pressure, to afford 25 mg of the title compound as yellow crude crystals.
1H-NMR (400 MHz, CD3OD)δ3.98 (2H, t, J = 4.4 Hz), 4.25 (2H, t, J = 4.4 Hz) , 7.0 6-7.14 (2H, m) , 7.28 (1H, d, J = 8.8 Hz) , 7.54 (1H, d, J = 16.6 Hz), 7.68-7.74 (2H, m) , 7.86 (1H, d, J = 16.6 Hz), 7.89 (1H, d, J = 8.8 Hz). ESI-MS: m/z = 341 (M-H)~
Example 351
From 3-[(E)-2-(4-fluorophenyl)-vinyl]-4- (2-hydroxyethoxy) -1H-indazole-5-carboxylie acid and various kinds of amine, compounds of Examples 352-356 were obtained in accordance with the method of Example 102. Example 352
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-1H-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI) m/z 428 MH+ Example 353
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-lH-ind azole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 382 MH+ Example 354
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide 1H-NMR (400 MHz, CD3OD) δ 3.82 (2H, t, J = 4.4 Hz), 4.08 (2H, t, J = 4.4 Hz), 4.62 (2H, s), 6.35 (1H, dd, J = 0.8, 3.6 Hz), 6.38 (1H, dd, J = 2.0, 3.6 Hz), 7.06-7.14 (2H, m) , 7.33 (1H, d, J = 8.8 Hz), 7.46 (1H, dd, J = 0.8, 2.0 Hz), 7.53 (1H, d, J = 16.6 Hz) , 7.64-7.72 (2H, m), 7.74 (1H, d, J = 16.6 Hz) , 7.85 (1H, d, J = 8.8 Hz). MS (ESI) m/z 422 MH+ Example 355 3-[ (E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)~1H-
indazole-5-carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide
MS (ESI) m/z 462 MH+ Example 356
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(2-hydroxyethoxy)~1H-indazole-5-carboxylic acid [(15)-1-carbamoylethyl]-amide 1H-NMR (400 MHz, CD3OD) δ 1.52 (3H, d, J = 6.8 Hz), 3.94-4.06 (2H, m), 4.15-4.25 (2H, m), 4.60-4.67 (1H, m), 7.08-7.16 (2H, m) , 7.34 (1H, d, J=8.8Hz), 7.55 (1H, d, J=16.2Hz), 7.66-7.74 (2H, m), 7.75 (1H, d, J = 16.2 Hz), 7.91 (1H, d, J = 8.8 Hz) . MS (ESI) m/z 413 MH+ Example 357
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-5-carboxylic acid
By treating 51.6 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-5-carboxylic acid ethyl ester obtained by Example 345 in the method according to Example 216, 61. 8 mg of the title compound was obtained as brown crude crystals . MS (ESI) m/z 297 MH" Example 358
From 3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-5-carboxylic acid and various kinds of amine, compounds of Examples 359-363 were obtained in accordance with the method of Example 102. Example 359 3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-5-
k
carboxylic acid [(15)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI) m/z 385 MH+ Example 360
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-1H-indazole-5-carboxylic acid cyclopropyl amide MS (ESI) m/z 338 MH+ Example 361
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
1H-NMR (400 MHz, CD3OD) δ 4.58 (2H, s), 6.30-6.33 (1H, m) , 6.35-6.38 (1H, m) , 6.93 (1H, d, J = 8.8 Hz) , 7.08-7.16 (2H, m) , 7.42-7.4 5 (1H, m) , 7.56-7.68 (2H, m) , 7.62 (1H, d, J = 16.8 Hz) , 7.65 (1H, d, J = 8.8 Hz), 7.68 (1H, d, J = 16.8 Hz). MS (ESI) m/z 378 MH+ Example 362
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-1H-indazole-5-carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide MS (ESI) m/z 418 MH+ Example 363
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-hydroxy-1H-indazole-5-carboxylic acid [(1S)-1-carbamoylethyl]-amide 1H-NMR (400 MHz, CD3OD) δ 1.51 (3H, d, J = 7.2 Hz), 4.62 (1H, q), 6.96 (1H, d, J = 9.2 Hz), 7.05 - 7.20 (2H, m), 7.46-7.72 (4H, m), 7.77 (1H, d, J = 9.2 Hz). MS (ESI) m/z 369 MH+ Production example 364
Acetic acid 2-(N'-benzyloxycarbonyl-hydrazino)-2-oxo-ethyl ester
To a solution of 2.04 g of hydrazine carboxylic acid benzyl ester in 40 mL of dichloromethane was added 2.57 mL of triethylamine. Under ice cooling, a solution of 1.32 mL of acetoxyacetylchloride in 20 mL of dichloromethane was added dropwise over 25 minutes, and stirred at room temperature for 1 hour. The reaction solution was added with water, extracted with ethyl acetate, and the resultant organic layer was washed with 1N hydrochloric acid, saturated aqueous sodium hydrogen carbonate and saturated brine, and then dried over magnesium sulfate. The solvent was evaporated, and the resulting solid product was washed with diethyl ether and collected by filtration, to give 1.92 g of the title compound as white crystals. 1H-NMR (400 MHz, DMSO-D6) δ 2.09 (3H, s), 4.52 (2H, s) , 5.07 (2H, s), 7.26-7.40 (5H, m), 9.25 (1H, bs), 9.95 (1H, bs). Production example 365 Acetic acid hydrazinocarbonyl ethyl ester
1. 92 g of acetic acid 2- (N-benzyloxycarbonyl-hydrazino) -2-oxo-ethyl ester was dissolved in 20 mL of ethanol, and added with 900 mg of 10% palladium on carbon. At room temperature, the solution was stirred for 5 hours under 1 atm hydrogen atmosphere, and filtrated through Celite, and the filtrate was evaporated, to afford 0.96 g of the title compound as a pale yellow oil. 1H-NMR (400 MHz, DMSO-D6) δ 2.07 (3H, s), 4.27 (2H, bs), 4.42
(2H, s), 9.20 (1H, bs).
Production example 366
N'-[2-(tert-Butoxycarbonyl-methyl-amino)-acetyl]-hydrazine
carboxylic acid benzyl ester
6.95 g of 2-(tert-butoxycarbonyl-methyl-amino)-acetic acid, 6.1 g of hydrazine carboxylic acid benzyl ester, 6.18 g of 1-hydroxybenzotriazole monohydrate and 19.2 mL of N,N-diisopropylethylamine were dissolved in 120 mL of N,N-dimethylformamide, and 10.6 g of
l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride was added under stirring at room temperature. After stirring at room temperature for 17 hours, the reaction solution was added with water and extracted with ethyl acetate, and the resultant organic layer was washed with saturated brine and dried over magnesium sulfate. The solvent was evaporated, followed by purification by silica gel column chromatography (hexane: ethyl acetate =1:1), to obtain 10.9 g of the title compound. 1H-NMR (400 MHz, DMSO-D6) δ1. 25-1. 46 (9H, m) , 2.68-2.86 (3H, m) , 3.8 0 (2H, d, J = 16.8 Hz) , 5.07 (2H, s) , 7.25-7.4 6 (5H, m) , 9.21
(1H, bs), 9.76 (1H, bs). Production example 367
Hydrazine carbonylmethyl-methyl-carbamic acid tert-butyl ester From 10 . 9 g of N'-[2-(tert-butoxycarbonyl-methyl-amino)-acetyl]-hydrazine carboxylic acid benzyl ester, 6.64 g of the title compound was obtained in accordance with Production example 365.
1H-NMR (400 MHz, DMSO-D6) δ 1.36 (9H, d, J = 19.6 Hz) , 2.77 (3H,
d, J = 14.8 Hz), 3.69 (2H, d, J = 10.0 Hz), 4.18 (2H, bs), 8.98
(1H, d, J = 11.6 Hz).
Production example 368
3-Iodo-7-fluoro-l-trityl-lH-indazole-5-carbonitrile
By treating 12 . 8 g of 7-fluoro-1H-indazole-5-carbonitrile obtained by Production example 120 in the similar method as described in Production example 206, followed by treatment in the similar method as describe in Production example 22, 21.2 g of the title compound was obtained as pale brown crystals. 1H-NMR (4 00MHz, CDCl3) δ 7.03 (1H, dd, J=10.4, 1.2 Hz) , 7.07-7.38 (15H, m), 7.72 (1H, d, J = 1.2 Hz). Example 369
7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carbonitrile
By treating 1.03 g of 3-bromo-7-fluoro-1-trityl-lH-indazole-5-carbonitrile in the similar method as described in Example 100, 348 mg of the title compound was obtained as pale yellow crystals.
1H-NMR (400MHz, DMSO-D6) δ 7.14 (1H, t, J =9.2 Hz), 7.14-7.47 (1H, m), 7.56-7.71 (4H, m), 7.77 (1H, d, J = 10.4 Hz). Example 370
7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-carboximidic acid ethyl ester hydrochloride
4.88 g of 7-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-carbonitrile was dissolved in 100 mL of ethanol, and
under stirring and ice cooling, hydrogen chloride was bubbled thereinto for 20 minutes. After stirring at room temperature for 19 hours, the solvent was evaporated, and the generated crystals were washed with diethyl ether, and collected by filtration, to afford 2. 86 g of the title compound as pale yellow crystals.
1H-NMR (400 MHz, CD3OD) δ 1.68 (3H, t, J = 6.8 Hz), 4.69 (2H, q, J= 6.8 Hz), 7.07 (1H, dt, J = 1.2, 8.0 Hz) , 7.38-7.52 (3H, m) , 7.57 (1H, d, J = 16.4 Hz), 7.68 (1H, d, J = 16.4 Hz), 7.77 (lh, dd, J = 1.2, 7.2 Hz), 8.81 (1H, s). Example 371
7-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboximidic acid ethyl ester hydrochloride, 3 equivalents of commercially available hydrazide or 3 equivalents of hydrazide obtained in Production example 365, and 3 equivalents of triethylamine were dissolved in ethanol, and stirred at 80°C for 18 hours. After distilling off the solvent, purification by LC-MS was performed to obtain compounds of Examples 372-374. Example 372
7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-5- (5-methyl-4H-[l,2,4]triazol-3-yl)-1H-indazole MS (ESI)m/z 338 MH+ Example 373
(5-{7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1,2, 4]triazol-3-yl)-methanol MS (ESI)m/z 354 MH+
Example 374
(5-{7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-yl}-4H-[1,2,4]triazol-3-ylmethyl)-dimethyl-amine MS (ESI)m/z 381 MH+ Example 375
(5-{7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1,2,4]triazol-3-ylmethyl)-methyl-amine
7-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-carboximidic acid ethyl ester hydrochloride obtained in Example 370, 3 equivalents of hydrazide obtained in Production example 367 and 3 equivalents of triethylamine were dissolved in ethanol, and stirred at 80°C for 18 hours. After distilling off the solvent, treatment with trifluoroacetic acid and purification by LC-MS were performed to afford the title compound.
MS (ESI)m/z 367 MH+ Example 37 6
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride
From 100 mg of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl] -lH-indazole-5-carbonitrile obtained by Example 100, 120 mg of the title compound was obtained as pale yellow crystals in accordance with the method of Example 370. 1H-NMR (400 MHz, DMSO-D6) δ 1.51 (3H, t, J =6.8 Hz), 4.66 (2H, q, J = 6.8 Hz) , 7.10-7.22 (1H, m) , 7.41-7.4 8 (1H, m) , 7.56 (1H, d, J = 8.0 Hz), 7.60-7.69 (4H, m), 8.83 (1H, d, J = 6.8 Hz).
Example 377
From 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazole-5-carboximidic acid ethyl ester hydrochloride compounds of Examples 378-381 were obtained in accordance with the method of Example 371 or 375. Example 37 8
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-5-(5-methyl-4H-[1,2,4]triazol-3-yl)-lH-indazole MS (ESI)m/z 338 MH+ Example 37 9
(5-{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H-[l,2, 4]triazol-3-yl)-methanol MS (ESI)m/z 354 MH+ Example 380
(5-{6-Fluoro-3-[{E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H-[1,2,4]triazol-3-ylmethyl)-dimethyl-amine MS (ESI)m/z 381 MH+ Example 381
(5-{6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1, 2, 4]triazol-3-ylmethyl)-methyl-amine MS (ESI)m/z 367 MH+ Example 382
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carbonitrile
From 5.15 g of 3-iodo-7-fluoro-l-trityl-2H-indazole-5-carbonitrile obtained by Production example 368 and
4-f luoro-styrene, 1.68 g of the title compound was obtained in
the similar method as described in Example 100.
1H-NMR (400 MHz, DMSO-D6) δ 7.24 (2H, t, J =8.8 Hz), 7.54 (1H,
d, J = 16.8 Hz), 7.64 (1H, d, J = 16.8 Hz), 7.74 (1H, d, J =
10.8 Hz), 7.81 (2H, dd, J = 8.8, 5.6 Hz), 8.76 (1H, s).
Example 383
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-
carboximidic acid ethyl ester hydrochloride
From 1.68 g of 7-fluoro-3-[(E) -2-(4-fluorophenyl)-vinyl] -1H-indazole-5-carbonitrile, 1. 81 g of the title compound was obtained in accordance with Example 370.
1H-NMR (400 MHz, DMSO-D6) δ 1.52 (3H, t, J =7.2 Hz), 4.64 (2H, q, J= 7.2 Hz), 7.25 (2H, t, J= 8.8 Hz), 7.54 (1H, d, J = 16.8 Hz), 7.82-7.93 (4H, m), 9.20 (1H, s). Example 384
From 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboximidic acid ethyl ester hydrochloride, compounds of Examples 385-387 were obtained in accordance with the method of Example 371 or 375. Example 385
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(5-methyl-4H-[1,2,4]triazol-3-yl)-lH-indazole MS (ESI)m/z 338 MH+ Example 386
(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H-[1,2,4]triazol-3-yl)-methanol
MS (ESI)m/z 354 MH+
Example 387
(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-4H-[1,2,4]triazol-3-ylmethyl)-methyl-amine
MS (ESI)m/z 367 MH+
Production example 388
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-l-trityl-lH-
indazole-5-carboxylic acid ethyl ester
By coupling 3.21 g of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 2.3 g of 2-[(E) -2- (3-fluorophenyl)-vinyl]-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane obtained by Production example 137 in accordance with the method of Example 194, 1.72 g of the title compound was obtained as colorless crystals.
1H-NMR ( 400 MHz, CDCl3 ) δ1.38 (3H, t, J = 7.4 Hz), 4.04 (3H, s), 4.36 (2H, q, J = 7.4Hz), 6.11 (1H, d, J=9.0Hz), 6.91-6.96 (1H, m) , 7.14-7.4 6 (19H, m) , 7.4 3 (1H, d, J = 9.0 Hz) , 7.61 (1H, d, J = 16.4 Hz). Production example 389
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid
By hydrolyzing 1.61 g of 3-[ (E) -2-(3-fluorophenyl)-vinyl]-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester in accordance with the method of Example 350, 1.64 g of the title compound was obtained as orange crude crystals.
1H-NMR (4 00 MHz, CD3OD)δ4.04 (3H, s) , 6.19 (1H, d, J = 9.2 Hz) ,
6.97-7.01 (1H, m), 7.17-7.41 (20H, m), 7.66 (1H, d, J = 16.4
Hz) .
Production example 390
N'-{3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-l-trityl-lH-
indazole-5-carbonyl}-hydrazine carboxylic acid tert-butyl
ester
94 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid and 34.4 mg of hydrazine carboxylic acid tert-butyl ester were condensed in the manner as described in Example 127, to afford 126.9 mg of the title compound as pale yellow crude crystals.
1H-NMR (400MHz, CDC13)δ1.50 (9H, s), 4.09 (3H, s), 6.22 (1H, d, J = 9.0 Hz) , 6.75 (1H, bs) , 6.92-6.98 (1H, m) , 7.15-7.33 (18H, m) , 7.4 0 (1H, d, J = 16.2 Hz) , 7.5 0 (1H, d, J = 16.2 Hz) , 7.63 (1H, d, J = 9.0 Hz), 9.40 (1H, bs) . Example 391
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid hydrazide
113.7 mg of N'-{3-[(E)-2-(3-fluorophenyl)-vinyl]-4-methoxy-l-trityl-lH-indazole-5-carbonyl}-
hydrazinecarboxylic acid tert-butyl ester was deproteced in the manner as described in Example 16, to afford 38 mg of the title compound as colorless crude crystals.
1H-NMR (400 MHz, DMSO-D6) δ 3.96 (3H, s), 7.10-7.14 (1H, m) , 7.35 (1H, d, J= 8.8 Hz), 7.4 0-7.52 (3H, m), 7.54 (1H, d, J =
16.4 Hz) , 7.59 (1H, d, J = 16.4 Hz) , 7. 66 (1H, d, J = 8.8 Hz) ,
10.55 (1H, s), 13.49 (1H, bs) .
Example 392
3-[(E)-2-(3-Fluorophenyl)-vinyl]-4-methoxy-5-(5-methyl-2H-
[1, 2, 4]triazol-3-yl)-lH-indazole
To a suspension of 19 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid hydrazide in 4 mL of ethanol were added 50 mg of O-methyl acetimidate hydrochloride and 0.19 mL of triethylamine, and stirred in a sealed tube at 150°C for 17.5 hours. The reaction solution was allowed to cool to room temperature, the solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography, and further purified and separated by LC-MS, to afford 1.17 mg of the title compound as a yellow amorphous.
1H-NMR (400 MHz, CD3OD) δ 2.62 (3H, s) , 3.89 (3H, s), 7.02-7.08 (1H, m), 7.37-7.4 4 (3H, m), 7.4 7 (1H, d, J = 8.8 Hz), 7.62 (1H, d, J = 16.4 Hz), 7.67 (1H, d, J = 16.4 Hz), 7.89 (1H, d, J = 8.8 Hz).
MS (ESI)m/z 392 MH+ Production example 393
6-Fluoro-3-[(E) -2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole-5-carboxylic acid methyl ester
In accordance with method of Production example 181, 576 mg of 3-bromo-6-fluoro-l-trityl-lH-indazole-5-carboxylic acid methyl ester obtained by Production example 141 and 235 mg of
3-vinylpyridine were made to react, to afford 312 mg of the title
compound as bright yellow crystals.
1H-NMR (400 MHz, DMSO-D6) δ 3.86 (3H, s) , 6.01 (1H, d, J = 12.4
Hz), 7.16-7.25 (6H, m), 7.32-7.46 (11H, m), 7.76 (1H, d, J =
16.8 Hz), 8.21 (1H, d, J= 8.4 Hz), 8.49 (1H, dd, J = 1.2, 8.8
Hz), 8.77 (1H, d, J = 6.8 Hz), 8.88 (1H, d, J = 1.2 Hz).
Production example 394
6-Fluoro-3-[(E) -2-(pyridin-3-yl)-vinyl]-1-trityl-lH-
indazole-5-carboxylic acid
From 187 mg of 6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole-5-carboxylic acid methyl ester, 187 mg of the title compound was obtained as ocher crystals in accordance with the method of Example 144.
1H-NMR (4 00 MHz, DMSO-D6) δ 5.99 (1H, d, J = 12.0 Hz) , 7.16-7.25 (6H, m), 7.30-7.46 (11H, m), 7.74 (1H, d, J = 16.4 Hz), 8.21 (1H, d, J = 8.4 Hz), 8.48 (1H, d, J = 4.0 Hz), 8.75 ( 1H, d, J = 7.2 Hz), 8.88 (1H, s), 13.20 (1H, bs) . Production example 395
6-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-1H-indazole-5-carboxylic acid hydrazide
In accordance with the method as described in Example 127, 110 mg of 6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole-5-carboxylic acid and 210 mg of hydrazine monohydrate were condensed, to afford 29 mg of the title compound as a yellow powder. 1H-NMR (400 MHz, DMSO-D6) δ 4.50 (2H, s), 6.01 (1H, d, J =11.6
Hz), 7.16-7.26 (6H, m), 7.28-7.46 (10H, m), 7.48 (1H, d, J =
16.8 Hz), 7.74 (1H, d, J = 16.8 Hz) , 8.20 (1H, d, J=7.6Hz),
8.42 (1H, d, J = 7.2 Hz), 8.48 (1H, d, J = 4.0 Hz), 8.87 (1H,
s), 9.57 (1H, s) .
Production example 396
6-Fluoro-5-(5-methyl-4H-[1,2,4]triazol-3-yl)-3-[(E)-2-
(pyridin-3-yl) -vinyl] -1-trityl-2H-indazole
To a solution of 28 mg of trityl-lH-indazole-5-carboxylic acid hydrazide in 2 mL of methanol was added 11 mg of S-methyl thioacetimidate hydriodate, and stirred at room temperature for 20 minutes. Then the reaction solution was added with 50 ul of triethylamine, and stirred at 60-75°C overnight. The reaction solution was added with 15 mL of ethyl acetate, and the organic layer was washed successively with water, saturated aqueous sodium hydrogen carbonate and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography (methanol :chloroform=l:4 9), to afford 16 mg of the title compound as white non-crystalline powder. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 2.40 (3H, s) , 6.07 (1H, d, J = 12.0 Hz), 7.16-7.26 (6H, m), 7.28-7.46 (11H, m), 7.73 (1H, d, J = 16.4 Hz), 8.21 (1H, d, J = 8.4 Hz), 8.48 (1H, d, J = 4.8 Hz), 8.67 (1H, d, J = 7.2 Hz), 8.87 (1H, s), 13.80 (1H, bs). Example 397
6-Fluoro-5-(5-methyl-4H-[1,2,4]triazol-3-yl)-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole
16 mg of 6-fluoro-5-(5-methyl-4H- [1,2,4]triazol-3-yl)-3-[ (E) -2- (pyridin-3-yl) -vinyl] -1-trityl-lH-indazole was deprotected in accordance with Example 16, and then purified and separated by LC-MS, to afford 4.7 mg of the title compound. MS (ESI) m/z 321 MH+ Example 3 98
7-Fluoro-5-(5-methyl-4H-[1,2,4]triazol-3-yl)-3- [ (E) -2-(pyridin-3-yl)-vinyl]-1H-indazole
7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole-5-carbonitrile obtained by Production example 124 was deprotected in the similar method as described in Example 16, followed by reactions in accordance with Examples 370 and 372, and purification and isolation by LC-MS, to afford the title compound.
1H-NMR (400 MHz, DMSO-D6) δ 2.40 (3H, s) , 7.41 (1H, dd, J =8.0, 4.8 Hz) , 7.52 (1H, d, J = 16.4 Hz), 7.74 (1H, d, J = 12.4 Hz) , 7.81 (1H, d, J = 16.4 Hz), 8.23 (1H, d, J = 8.0 Hz), 8.47 (1H, d, J = 4.8 Hz), 8.53 (1H, s), 8.89 (1H, s). Example 399
(5-{7-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-AH-[1,2,4]triazol-3-ylmethyl)dimethylamine
After removing the trityl group from 7-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-l-trityl-1H-indazole-5-carbonitrile obtained by Production example 124 in accordance with the method of Example 16, reactions as described in Examples 370 and 374 as well as separation and purification by LC-MS were followed,
to obtain the title compound.
1H-NMR (400 MHz, DMSO-D6) δ 2.25 (6H, s), 3.62 (2H, s), 7.43 (1H, dd, J = 8.0, 4.4 Hz) , 7.54 (1H, d, J = 16.8 Hz) , 7.78 (1H, d, J = 12.0 Hz), 7.83 (1H, d, J = 16.8 Hz), 8.24 (1H, dt, J = 8.0, 1.6Hz), 8.49 (1H, dd, J=4.4, 1.6Hz), 8.56 (1H, s) , 8.90 (1H, d, J = 1.6 Hz). Example 4 00
4-Methoxy-5-(5-methyl-2H-[1,2,4]triazol-3-yl)-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole
4-Methoxy-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazole-5-carboxylic acid obtained by Production example 326 was led to hydrazide in accordance with Production examples 390 and 391. A suspension of 30 mg of hydrazide in 5 mL of ethanol was added 32 mg of s-methyl thioacetimidate hydriodate, and stirred at room temperature for 1 hour. Then the reaction solution was added with 0.069 mL of triethylamine, and allowed to react at 150°C for 30 minutes under microwave irradiation. The reaction mixture was purified and separated by LC-MS, to obtain 2.04 mg of the title compound and 1.44 mg of 4-methoxy-5- (5-methyl-2H-[1,3,4]oxadiazol-2-yl)-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole as described in Example 401 below.
1H-NMR (400 MHz, DMSO-D6) δ2 . 60 (3H, s) , 3.88 (3H, s) , 7.43-7.73 (1H, m), 7.70-7.77 (2H, m), 7.86-7.96 (4H, m) , 8.61-8.71 (2H, m) , 8.95-8.99 (1H, m) . MS (ESI) m/z 333 MH+ Example 401
4-Methoxy-5-(5-methyl-2H- [1,3,4]oxadiazol-2-yl)-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazole
1H-NMR ( 4 00 MHz, DMSO-D6 ) δ2 . 67 (3H, s) , 4.00 (3H, s) , 7.09 (1H, d, J = 8.4 Hz), 7.48 (1H, d, J = 8.8 Hz), 7.90-7.99 (4H, m), 8.66 (1H, bs), 8.76 (1H, d, J = 8.4 Hz), 9.01 (1H, d, J = 7.6 Hz) .
MS (ESI) m/z 334 MH+ Example 4 02 3- (3-Fluorophenyl) -5-methoxy-2H-pyrazolo [4 , 3-b] pyridine
35 mg of 3-(3-fluorophenyl)-5-chloro-l-trityl-lH-pyrazolo[4,3~b]pyridine obtained by Production example 37 was dissolved in 2 mL of methanol, added with 20 ul of triethylamine and 15 ul of ethyl chloroformate, and heated at reflux for 16 hours. The reaction solution was allowed to cool to room temperature, the solvent was evaporated, and the residue was dissolved in 3 mL of dichloromethane. After adding 0.5 mL of trifluoroacetic acid and stirring at room temperature for 30 minutes, the reaction solution was partitioned between saturated aqueous sodium hydrogen carbonate and ethyl acetate, extracted with ethyl acetate, and the organic layer was washed with water, and dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=3:7), to afford 7.25 mg of the title compound as a pale yellow powder.
1H-NMR (400MHz, CDCl3)δ4.08 (3H, s), 6.88 (1H, d, J= 9.1Hz), 7.08 (1H, dt, J = 2.5, 8.1 Hz), 7.46 (1H, dt, J = 6.2, 8.1 Hz),

7.71 (1H, d, J = 9.1 Hz), 8.29 (1H, bd, J = 8.1 Hz), 8.32 (1H,
bd, J = 11.0 Hz) .
Example 4 03
3-(Naphthalen-2-yl)-lH-pyrazolo[3,4-c]pyridine-5-carboxylie
acid
From 40 mg of 3-(naphthalen-2-yl)-1H-pyrazolo[3,4-c]pyridine-5-carbonitrile obtained by Example 75, 33 mg of the title compound was obtained as pale brown crystals in accordance with the method of Example 7.
1H-NMR (400 MHz, DMSO-D6) δ7 . 52-7 . 63 (2H, m) , 7.93-8.23 (4H, m) , 8.59 (1H, s), 8.88 (1H, s), 9.20 (1H, s). Production example 4 04
l-Benzyl-3- (naphthalen-2-yl) -1H-pyrazolo [3, 4-b]pyridine-5-carbonitrile
900 mg of 2-benzyl-5-(2-naphthyl)-2H-pyrazol-3-ylamine obtained from 3-(naphthalen-2-yl)-3-oxopropionitrile and benzyl hydrazine in the similar method as described in Production example 30 was subjected to the similar reaction as described in Production example 31, to afford 770 mg of the title compound as a pale yellow powder.
1H-NMR (400 MHz, DMSO-D6) δ5. 81 (2H, s) , 7.25-7.36 (5H, m) , 7.54-7.62 (2H, m) , 7.94-7.99 (1H, m) , 8.04 (1H, d, J = 8.7 Hz), 8.13-8.18 (1H, m), 8.19 (1H, dd, J = 2.0, 8.7 Hz), 8.70 (1H, d, J = 2.0 Hz), 9.03 (1H, d, J = 2.0 Hz), 9.53 (1H, d, J = 2.0 Hz) . Example 405
3- (Naphthalen-2-yl) -lH-pyrazolo [3, 4-jb]pyridine-5-carboxylic acid
500 mg of l-benzyl-3-(naphthalen-2-yl)-1H-pyrazolo[3, 4-jb]pyridine-5-carbonitrile obtained by Production example 404 was treated in the similar method as described in Example 437, to obtain 230 mg of the title compound as a pale brown powder in which deprotection and hydrolysis had proceeded, as well as 20 mg of 3-(naphthalen-2-yl)-lH-pyrazolo[3,4-b]pyridine-5-carbonitrile described in Example 406 as a pale brown powder. 1H-NMR (400 MHz, DMSO-D6) δ7.55-7.60 (2H, m), 8.08 (1H, d, J = 8.5 Hz) , 8.14-8.19 (1H, m) , 8.18 (1H, dd, J = 1.5, 8.5 Hz) , 8.59 (1H, s), 9.09 (1H, d, J = 1.5 Hz), 9.12 (1H, d, J = 1.5 Hz), 13.25-13.40 (1H, bs), 14.25 (1H, bs) . Example 4 06
3- (Naphthalen-2-yl) -1H-pyrazolo [3, 4-b]pyridine-5-carbonitrile
1H-NMR (400 MHz, DMSO-D6) δ7.55 (1H, bt, J =8.4 Hz), 7.58 (1H, bt, J = 8.4 Hz), 7.96 (1H, bd, J = 8.4 Hz), 8.05 (1H, d, J = 8.4 Hz), 8.15 (1H, bd, J=8.4Hz), 8.23 (1H, dd, J = 2.1, 8.4 Hz), 8.69 (1H, d, J = 2.1 Hz), 8.93 (1H, d, J = 2.1 Hz), 9.47 (1H, d, J = 2.1 Hz), 14.35-14.60 (1H, bs). Production example 407 N- (2-Methyl-pyridin-3-yl)-acetamide
Under nitrogen atmosphere, 47.6 g of 2-chloro-3-nitropyridine was dissolved in 500 mL of tetrahydrofuran, and 150 mL of 2M methylzinc chloride in
tetrahydrofuran and 6.9 g of
tetrakis(triphenylphosphine)palladium(0) were added, and the reaction solution was stirred at 70°C for 2 hours. The reaction solution was poured into cold water, extracted with ethyl acetate, washed with water, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the residue was subjected to column chromatography (n-hexane:ethyl acetate =3:1) to obtain 35.4 g of 2-methyl-3-nitropyridine as a colorless oil. Then 35.4 g of 2-methyl-3-nitropyridine was dissolved in a mixed solution of 300 mL methanol/5 mL triethylamine, added with 5 gof10% palladium on carbon, and stirred for 6 hours under hydrogen atmosphere and at normal temperature and pressure . The reaction solution was filtered thorough Celite, the solvent was evaporated, and 33.0 g of crudely produced 2-methyl-3-aminopyridine was obtained as a pale brown oil. Next, to 100 mL of a solution of 65 g of crude 3-amino-2-methyl pyridine in dichloromethane were added 60 mL of pyridine and 71 mL of acetic anhydride at room temperature and stirred for 3 hours. The reaction solution was added with about 150 mL of silica gel powder, the solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :methanol =100:3), to afford 74 g of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3)δ2.25 (3H, s) , 2.53 (3H, s), 7.00 (1H, bs), 7.18 (1H, dd, J = 4.6, 8.0 Hz), 8.23 (1H, d, J= 8.0Hz), 8.30 (1H, d, J = 4.6 Hz). Production example 4 08
1-Pyrazolo [4, 3-.ib] pyridin-1-yl-ethanone
To a solution of 74 g of N- (2-methyl-pyridin-3-yl)-acetamide obtained by Production example 407 in toluene were added 106 mL of isoamyl sulfite, 140 mL of acetic anhydride and 57.6 g of potassium acetate at room temperature, and the reaction solution was heated at 80°C for 2 hours. The reaction solution was partitioned into water and ethyl acetate, and the organic layer was extracted and dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=l:5), to afford 20 g of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3)δ2.83 (3H, s) , 7.48 (1H, dd, J = 4.7, 8.3 Hz), 8.37 (1H, s), 8.72 (1H, bd, J = 8.3 Hz), 8.73 (1H, d, J = 4.7 Hz) . Production example 4 09 lH-Pyrazolo [4, 3-Jb] pyridine
20 g of l-pyrazolo[4, 3-b]pyridin-1-yl-ethanone obtained by Production example 408 was dissolved in a mixed solution of 20 mL methanol/80 mL tetrahydrofuran, added with 10 mL of 2N sodiumhydroxide aqueous solution at room temperature and stirred for 0 . 5 hours . The reaction solution was neutralized by adding 2 mL of 5N hydrochloric acid, then added with about 50 mL of silica gel powder, and the solvent was evaporated. The residue was purified by silica gel column chromatography (ethyl acetate : methanol =100:2), to afford 14 . 6 g of the title compound
as pale yellow cubic crystals.
1H-NMR (400 MHz, CDCl3)δ7.35 (1H, dd, J = 4.3, 8.6 Hz), 7.89 (1H, d, J = 8.6 Hz), 8.36 (1H, bs), 8.64 (1H, dd, J = 1.5, 4.3 Hz) .
Production example 410 3-Bromo-lH-pyrazolo [4 , 3-b] pyridine
750 mg of N-bromosuccinimide was reacted on 0.5 g of lH-pyrazolo [4, 3-b]pyridine in the similar method as described in Production example 87, to afford 570 mg of the title compound as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ7.43 (1H, dd, J =4.3, 8.6 Hz), 8.07 (1H, dd, J=1.4, 8.6Hz), 8.59 (1H, dd, J=1.4, 4.3Hz), 13.68 (1H, bs).
Production example 411 3-Bromo-l-trityl-1H-pyrazolo [4, 3-b]pyridine
By treating 570 mg of 3-bromo-lH-pyrazolo [4, 3—Jb]pyridine obtained by Production example 410 in the similar method as described in Production example 22, 860 mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ6.73 (1H, d, J = 8.8 Hz), 7.14-7.19 (6H, m) , 7.20 (1H, dd, J = 4.1, 8.8 Hz) , 7.28-7.38 (9H, m) , 8.51 (1H, dd, J = 4.1 Hz). Production example 412 3-Bromo-l-trityl-lH-pyrazolo[4,3-b]pyridine 4-oxide
By treating 110 mg of 3-bromo-l-trityl-lH-pyrazolo [4, 3-b]pyridine in the similar
method as described in Production example 4, 70 mg of the title
compound was obtained as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ6.25 (1H, d, J = 8.8 Hz), 7.06 (1H,
dd, J = 6.4, 8.8 Hz) , 7.12-7.17 (6H, m) , 7.28-7.38 (9H, m) , 8.08
(1H, d, J = 6.4 Hz).
Production example 413
3-Bromo-l-trityl-lH-pyrazolo [4, 3-b]pyridine-5-carbonitrile
By treating 300 mg of 3-bromo-l-trityl-lH-pyrazolo [4, 3-b]pyridine 4-oxide in the same manner as described in Production example 5, 240 mg of the title compound was obtained as a colorless powder. 1H-NMR (400MHz, CDCl3)δ6.65 (1H, d, J=9.0Hz), 7.14-7.18 (6H, m), 7.27 (1H, d, J = 9.0 Hz), 7.29-7.35 (9H, m). Production example 414
3-[(E)-2-(3-Fluorophenyl)-vinyl]-1-trityl-1H-pyrazolo[4,3-b ]pyridine-5-carbonitrile
By treating 180 mg of 3-bromo-l-trityl-lH-pyrazolo [4, 3-b]pyridine-5-carbonitrile obtained by Production example 413 in the similar method as described in Example 194, 180 mg of a crude product of the title compound as a colorless oil.
1H-NMR (400MHz, CDCl3)δ6.66 (1H, d, J = 8.5Hz), 6.95-7.01 (1H, m) , 7.13-7.22 (7H, m) , 7.23-7.40 (12H, m) , 7.26 (1H, d, J = 8.5 Hz), 7.40 (1H, d, J = 16.6 Hz), 8.19 (1H, d, J = 16.6 Hz). Example 415 3- [ (E) -2- (3-Fluorophenyl) -vinyl] -1H-pyrazolo [4, 3-b] -
pyridine-5-carbonitrile
By treating 180 mg of the crude 3-[(E)-2-(3-f luorophenyl) -vinyl] -1-trityl-lH-pyrazolo [4, 3-b]pyridine-5-carbonitrile obtained by Production example 414 in the similar method as described in Example 16, 60 mg of the title compound was obtained as a pale yellow powder.
1H-NMR (400 MHz, CDCl3) δ7 . 00-7.06 (1H, m) , 7.35-7.40 (2H, m) , 7.40-7.45 (1H, m), 7.47 (1H, d, J = 16.9 Hz), 7.72 (1H, d, J = 8.5 Hz), 7.95 (1H, d, J = 8.5 Hz), 8.25 (1H, d, J = 16.9 Hz) . Example 416
3- [ (E) -2- (3-Fluorophenyl) -vinyl] -lH-pyrazolo [4 , 3-Jb] -pyridine-5-carboxylic acid
By treating 60 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-pyrazolo [4, 3-Jb] pyridine-5-carbonitrile in the similar method as described in Example 7, 36mg of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ7.14 (1H, bt, 8.5 Hz), 7.44 (1H, dt, 6.2, 7.7 Hz) , 7.50 (1H, bd, 7.7 Hz) , 7.57 (1H, bd, 8.5 Hz) , 7.61 (1H, d, J = 16.6 Hz), 8.09 (1H, d, J = 8.8 Hz), 8.14 (1H, d, J = 8.8 Hz), 8.19 (1H, d, J = 16.6 Hz), 13.07 (1H, bs), 13.68 (1H, bs).
Production example 417 3-Iodo-lH-pyrazolo[4,3-b]pyridine
In the similar method as described in Production example 206, 9.5 g of iV-iodosuccinimide was reacted on 5 g of lH-pyrazolo[4, 3-b] pyridine, toafford 5. 9 g of the title compound
as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ7 . 43 (1H, dd, J = 4.2, 8.2 Hz), 8.00
(1H, dd, J= 1.3, 8.2 Hz) , 8.53 (1H, dd, J= 1.3, 4. 2 Hz) , 13.64-13.83
(1H, bs).
Production example 418
3-Iodo-l-trityl-lH-pyrazolo [4, 3-Jb]pyridine
By treating 5.9 g of 3-iodo-lH-pyrazolo[4,3-b]pyridine obtained by Production example 417 in the similar method as described in Production example 22, 10. 7 g of the title compound was obtained as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ6.56 (1H, dd, J = 1.2, 8.7 Hz), 6.96 (1H, dd, J = 4.3, 8.7 Hz), 7.15-7.22 (6H, m), 7.25-7.35 (9H, m), 8.53 (1H, dd, J = 1.2, 4.3 Hz). Production example 419 3- (Naphthalen-2-yl) -1-trityl-lfl-pyrazolo [4, 3-b]pyridine
By treating 731 mg of 3-iodo-l-trityl-lH-pyrazolo [4, 3-b]pyridine obtained by Example 418 and 340 mg of 2-naphthaleneboronic acid in the manner as described in Production example 194, 210 mg of the title compound was obtained as a colorless powder.
The instrumental data coincided with that of Production example 13. Example 420 5-Chloro-3-naphthalen-2-yl-lH-pyrazolo[4,3-b]pyridine
After treating 210 mg of 3-(naphthalen-2-yl)-1-trityl-1H-pyrazolo[4,3-b]pyridine
obtained by Production example 419 in the similar method as
described in Production example 4, 2 mL of residue solution in
toluene was added at room temperature with 190 ul of phosphorus
oxychloride, and treated in the similar method as described in
Production example 28, to afford 60 mg of the title compound
as a pale yellow powder.
1H-NMR (400MHz, CDCl3)δ7.38 (1H, d, J=8.9Hz), 7.48-7.55 (2H,
m), 7.83 (1H, d, J = 8.9 Hz), 7.85-7.90 (1H, m), 7.97 (1H, d,
J = 8.9 Hz) , 8.01-8.06 (1H, m) , 8.52 (1H, dd, J = 1.8, 8.9 Hz) ,
9.08 (1H, d, J = 1.8 Hz).
Production example 421
5-Chloro-3-(naphthalen-2-yl)-1-trityl-lH-pyrazolo[4,3-
i>] pyridine
By treating 170 mg 5-chloro-3-(naphthalen-2-yl)-lH-pyrazolo[4,3-b]pyridine obtained by Example 420 in the similar method as described in Production example 22, 340 mg of the title compound was obtained as a colorless powder.
1H-NMR (400MHz, CDCl3)δ6.56 (1H, d, J =8.7 Hz), 6.94 (1H, d,
J = 8.7 Hz), 7.22-7.28 (6H, m), 7.28-7.36 (9H, m), 7.44-7.52
(2H, m) , 7.81-7.8 5 (1H, m) , 7.87 (1H, d, J = 8.7 Hz) , 7.97-8.03
(1H, m), 8.38 (1H, dd, J = 2.0, 8.7 Hz), 9.08 (1H, d, J = 2.0
Hz) .
Production example 422
3- (Naphthalen-2-yl) -1-trityl-1H-pyrazolo [4 , 3-b] pyridin-5-ylamine
280 mg of 5-chloro-3-(naphthalen-2-yl)-1-trityl-lH-pyrazolo [4, 3-i)]pyridine was dissolved in 8 mL of toluene, added with 73 mg sodium t-butoxide, 180 ul of benzophenone imine, 100 mg of 2,2'-bis(diphenylphosphino)-1,1'-binaphthyl and 56 mg of tris(dibenzylidene acetone)bispalladium, and stirred at 100°C for 1.5 hours. The reaction solution was allowed to cool to room temperature, the solvent evaporated, and the residue was added with 5 mL of tetrahydrofuran and 0. 3 mL of 5N hydrochloric acid and stirred at room temperature for 1 hour. The resultant reaction solution was added with aqueous sodium hydrogen carbonate, extracted with ethyl acetate, and the organic layer was washed with water and dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate : hexane=l: 3) , to afford 220 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ4.47-4. 63 (2H, bs) , 6.25 (1H, d, J = 9.1 Hz) , 6.43 (1H, d, J = 9.1 Hz) , 7.24-7.33 (15H, m) , 7.39-7.4 8 (2H, m) , 7.78-7.82 (1H, m) , 7.83 (1H, d, J = 8.8 Hz) , 7.92-7.96 (1H, m), 8.36 (1H, dd, J =1.6, 8.8 Hz), 9.06 (1H, d, J = 1.6 Hz) .
Production example 423
Cyclopropane carboxylic acid [3-(naphthalen-2-yl)-1-trityl-lH-pyrazolo[4,3-b]pyridin-5-yl]amide
20 mg of 3- (naphthalen-2-yl) -1-trityl-lH-pyrazolo [4, 3-b] pyridin-5-ylamine was dissolved in 3 mL of toluene, and added with 20 µl of triethylamine and 10 µl of
cyclopropanecarbonyl chloride at room temperature and stirred all day and night. The reaction solution was added with saturated aqueous sodiumhydrogen carbonate, extracted with sodiumacetate, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=l:4), to afford 20 mg of the title compound as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ0 . 88-0 . 95 (2H, m) , 1.03-1.12 (2H, m) , 1.57-1.65 (1H, m), 6.64 (1H, d, J = 9.4 Hz), 7.23-7.33 (15H, m), 7.42-7.51 (2H, m), 7.80-7.86 (1H, m), 7.86 (1H, d, J=8.4 Hz), 7.92-7.98 (1H, m) , 7.96 (1H, d, J = 9.4 Hz), 8.35 (1H, dd, J = 1.8, 8.4 Hz), 8.45 (1H, bs), 9.01 (1H, d, J = 1.8 Hz). Example 424
Cyclopropane carboxylic acid [3-(naphthalen-2-yl)-1H-pyrazolo [4 , 3-b] pyridin-5-yl] amide
By deprotecting 20 mg of cyclopropane carboxylic acid (3-naphthalen-2-yl-l-trityl-lH-pyrazolo[4,3-b]pyridin-5-yl) amide obtained by Production example 423 in the similar method as described in Example 16, 9.1 mg of the title compound was obtained as a colorless powder.
1H-NMR (400MHz, CD3OD) δ0.89-0.95 (2H, m), 1.00-1.15 (2H, m), 1.96-2.04 (1H, m), 7.45-7.54 (2H, m), 7.84-7.90 (1H, m), 7.93 (1H, d, J = 8.9 Hz), 7.97 (1H, d, J = 9.2 Hz), 7.97-8.03 (1H, m), 8.25 (1H, d, J = 9.2 Hz), 8.49 (1H, dd, J = 1.9, 8.9 Hz), 9.11 (1H, s) . Production example 425
2-Iodo-4-methyl-5-nitropyridine
To a solution of 1. 0 g of 2-amino-4-methyl-5-nitropyridine in 10 mL of diiodomethane was added 1.8 mL of isopentyl sulfite at room temperature, stirred at this temperature for 30 minutes, and after raising the temperature to 80°C, the solution was stirred for 2 hours. The diiodomethane was evaporated, and the crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:20), to afford 897 mg of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ2.58 (3H, s), 7.77 (1H, s), 8.90 (1H, s) .
Production example 426 6-Iodo-4-methylpyridin-3-ylamine
To a solution of 1.76 g of 2-iodo-4-methyl-5-nitropyridine in 7 . 0 mL of concentrated hydrochloric acid and 7. 0 mL of diethyl ether was added 6. 32 g of tin chloride (II) at room temperature, and stirred at 100°C for 4 hours. After cooling the solution to room temperature, 5N sodium hydroxide aqueous solution was added to make the solution alkaline. Then the solution was diluted with dichloromethane and the insoluble substances were filtered off through Celite. The organic layer of the filtrate was dried over anhydrous magnesium sulfate, and the solvent was evaporated, to afford 897 mg of the title compound as orange-brown crystals.
1H-NMR (400MHz, CDCl3)δ2.10 (3H, s), 3.61 (2H, bs), 7.33 (1H, s) , 7.80 (1H, s) .
Production example 427
N-(6-Iodo-4-methylpyridin-3-yl)acetamide
By treating 1.55 g of 6-iodo-4-methylpyridin-3-ylamine obtained by Production example 426 in the similar method as described in Production example 17 6, 1. 68 g of the title compound was obtained as colorless crystals.
1H-NMR (400 MHz, CDCl3)δ2.21 (3H, s) , 2.22 (3H, s) , 6.89 (1H, bs), 7.56 (1H, s), 8.62 (1H, s). Production example 428 1-(5-Iodo-pyrazolo[3,4-c]pyridin-l-yl)ethanone
By treating 1.68 g of N-(6-iodo-4-methylpyridin-3-yl)acetamide in the similar method as described in Production example 408, 1. 58 gof the title compound was obtained as colorless crystals.
1H-NMR (4 00 MHz, CDCl3) δ2.80 (3H, s) , 8.10 (2H, d, J = 0.8 Hz) , 9.56 (1H, t, J = 0.8 Hz). Production example 429 lH-Pyrazolo[3,4-c]pyridine-5-carbonitrile
A suspension of 6.40 g of 1-(5-iodo-pyrazolo [3,4-c]pyridin-l-yl)ethanone obtained by Production example 428, 5.24 g of zinc cyanide and 2.58 g of
tetrakis(triphenylphosphine)palladium(O) in 70 mL of N,N-dimethylformamide was stirred at 40°C for 2 days. The suspension was diluted with ethyl acetate, and the insoluble substances were filtered through Celite. The filtrate was washed successively with saturated ammonium chloride and
saturated brine, and then dried over anhydrous magnesium sulfate.
The solvent was evaporated, and the crude product was purified
and separated by silica gel column chromatography (ethyl
acetate :n-hexane=l:10-1:1), to afford 2.78 g of the title
compound as colorless crystals.
1H-NMR (400 MHz, CD3OD)δ8.34 (1H, d, J = 0.8 Hz), 8.38 (1H, d,
J = 0.8 Hz), 9.09 (1H, t, J = 0.8 Hz).
Production example 430
3-Iodo-1H-pyrazolo[3,4-c]pyridine-5-carbonitrile
From 2 . 78 g of lH-pyrazolo [3, 4-c] pyridine-5-carbonitrile, 5.89 g of the title compound (N,N-dimethylformamide still remaining) was obtained as colorless crystals in accordance with the method of Production example 206.
1H-NMR (400 MHz, CD3OD)δ8.08 (1H, d, J = 0.8 Hz), 9.05 (1H, t, J = 0.8 Hz). Production example 431 3-Iodo-l-trityl-lH-pyrazolo[3,4-c]pyridine-5-carbonitrile
To a solution of 5.8 g of 3-iodo-1H-pyrazolo[3,4-c]pyridine-5-carbonitrile in 70 mL of dichloromethane were added 13.1 mL of diisopropylethylamine, 2.4 g of tetra-n-butylammonium iodide and 12.0 g of trityl chloride at room temperature, and stirred at this temperature for a day. The solution was evaporated, diluted with ethyl acetate, washed successively with saturated ammonium chloride and saturated brine, and dried over anhydrous magnesium sulfate . The solvent was evaporated, and the crude product was purified
and separated by silica gel column chromatography (ethyl
acetate :n-hexane=l:10), to afford 5.2 g of the title compound
as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ7. 10-7. 15 (6H, m) , 7.27-7.36 (9H, m) ,
7.60 (1H, d, J = 1.2 Hz), 7.84 (1H, d, J = 1.2 Hz).
Production example 432
3-[(E) -2-(3-Fluorophenyl)-vinyl]-1-trityl-lH-
pyrazolo[3,4-c]pyridine-5-carbonitrile
From 1.0 g of 3-iodo-l-trityl-2H-pyrazolo [3,4-c]pyridine-5-carbonitrile, 326 mg of the title compound was obtained as colorless crystals in accordance with the method of Example 194.
1H-NMR (400 MHz, CDCl3) δ6.98-7.04 (1H, m) , 7.15-7.36 (20H, m) , 7.73 (1H, d, J = 1.2 Hz), 8.30 (1H, d, J = 1.2 Hz). Example
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[3,4-c]pyridine-5-carboxylic acid
From 87 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-pyrazolo [3, 4-c] pyridine-5-carbonitrile obtained by Production example 432, the trityl group was deprotected in accordance with Example 16, followed by treatment in accordance with the method of Example 7, to thereby afford 55 mg of the title compound as pale yellow crystals.
1H-NMR (400MHz, DMSO-D6)δ7.11 (1H, td, J =8.0, 2.0Hz), 7.42 (1H, dd, J = 14.4, 8.0 Hz) , 7.57 (1H, d, J= 8.0Hz), 7.58 (1H, d, 16.4 Hz), 7.69 (1H, d, J = 10.4 Hz), 7.79 (1H, d, J = 14.4
Hz), 8.89 (1H, d, J = 1.2 Hz), 9.07 (1H, s).
Production example 434
2-Benzyl-5-(naphthalen-2-yl)-4-nitroso-2H-pyrazol-3-ylamine
600 mg of 2-benzyl-5-(2-naphthyl)-2H-pyrazol-3-ylamine obtained as an intermediate of Production example 404 was dissolved in a mixed solvent of 15 mL ethanol/0 . 5 mL concentrated hydrochloric acid, and 340 µl of isoamyl sulfite was added dropwise under ice cooling. The reaction solution was partitioned between saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the organic layer was extracted and washed with water. The organic layer was dried over magnesium sulfate, the solvent was evaporated, and the residue was purif iedby silica gel column chromatography (ethyl acetate :hexane=3: 7) , to afford 440 mg of the title compound as red-brown needle crystals. 1H-NMR (400 MHz, DMS0-D6) δ5.24 (2H, s) , 7.26-7.31 (3H, m) , 7.33-7.38 (2H, m), 7.51-7.58 (2H, m) , 7.92-7.96 (1H, m) , 7.97-8.01 (1H, m) , 7.99 (1H, d, J = 8.8 Hz), 8.26 (1H, dd, J = 1.6, 8.8 Hz), 8.57 (2H, bs), 8.88 (1H, s). Production example 435 2-Benzyl-5-(naphthalen-2-yl)-2H-pyrazole-3,4-diamine
200 mg of 2-benzyl-5-(naphthalen-2-yl)-4-nitroso-2H-pyrazol-3-ylamine obtained by Production example 434 was dissolved in a mixed solvent of methanol/tetrahydrof uran, added with 100 mg of palladium-carbon, and stirred for 2 hours under hydrogen atmosphere at normal temperature and normal pressure. The reaction solution was filtered through Celite, and the
solvent was evaporated, to afford 190 mg of the title compound
as yellow-brown needle crystals.
1H-NMR (400 MHz, DMSO-D6) δ3.45 (2H, bs), 4.77 (2H, bs), 5.16
(2H, s), 7.19 (2H, d, J = 7.1 Hz), 7.22 (1H, t, J = 7.1 Hz),
7.30 (2H, t, J = 7.1 Hz), 7.41 (1H, bt, J = 7.9 Hz), 7.45 (1H,
bt, J = 7.9Hz), 7.82 (1H, d, J=8.8Hz), 7.83 (1H, d, J=7.9
Hz), 7.86 (1H, d, J = 7.9 Hz), 8.05 (1H, dd, J = 1.6, 8.8 Hz),
8.38 (1H, dd, J = 1.6 Hz).
Production example 436
l-Benzyl-3-(naphthalen-2-yl)-1H-pyrazolo[3,4-b]pyrazine
190 mg of 2-benzyl-5-(naphthalen-2-yl)-2H-pyrazole-3,4-diamine was dissolved in 10 mL of methanol, added with 100 µl of 40% glyoxal aqueous solution, and stirred at room temperature for 5 hours . The reaction solution was partitioned between water and ethyl acetate, and the organic layer was extracted and washed with water. The organic layer was dried over magnesium sulfate, the solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate : hexane=l: 5) , to afford 180 mg of the title compound as a pale yellow powder. 1H-NMR (4 00MHz, CDCl3)δ5.80 (2H, s) , 7.26-7.36 (3H, m) , 7.42-7.47 (2H, m), 7.48-7.55 (2H, m), 7.85-7.90 (1H, m), 8.02 (1H, d, J = 8.4 Hz), 7.99-8.04 (1H, m), 8.53 (1H, d, J = 2.1 Hz), 8.55 (1H, dd, J = 1.7, 8.4 Hz), 8.70 (1H, d, J = 2.1 Hz), 9.08 (1H, s).
Example 437 3-Naphthalen-2-yl-lH-pyrazolo [3, 4-b]pyrazine
A mixture of 50 mg of l-benzyl-3-(naphthalen-2-yl)-1H-pyrazolo [3, 4-b]pyrazine obtained by Production example 436 and 1. 0 g of pyridine hydrochloride was heated at 200°C for 24 hours. The reaction solution was allowed to cool to room temperature, partitioned between water and ethyl acetate, extracted with ethyl acetate, and the organic layer was washed with water and dried over magnesium sulfate. The solvent was evapoarated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=3:7) , to afford 21 mg of the title compound as a pale yellow powder.
1H-NMR (400 MHz, DMSO-D6) δ7 . 52-7 . 59 (2H, m) , 7.93-7.98 (1H, m) , 8.02-8.07 (1H, m), 8.06 (1H, d, J = 9.0 Hz), 8.50 (1H, dd, J = 1.4, 9.0 Hz), 8.67 (1H, d, J = 2.0Hz), 8.78 (1H, d, J=2.0 Hz), 9.06 (1H, s), 14.27 (1H, bs) . Production example 4 38
N-[2-Benzyl-5-(naphthalen-2-yl)-4-nitroso-2H-pyrazole-3-ylamino]-maronamic acid ethyl ester
200 mg of 2-benzyl-5-(naphthalen-2-yl)-4-nitroso-2H-pyrazol-3-ylamine obtained by Production example 434 was dissolved in 10 mL of tetrahydrofuran, added with 300 ul of triethylamine and 270 ul of ethyl chlorocarbonylethyl acetate, and stirred at room temperature all day and night. The reaction solution was partitioned between water and ethyl acetate, and the organic layer was extracted and washed with water. The organic layer was dried over magnesium sulfate, the solvent was evaporated, and the residue was purified by silica gel column
chromatography (ethyl acetate :hexane=3:7) , to afford 2100 mg of the title compound as green needle crystals. 1H-NMR (400 MHz, DMSO-D6) δ1. 19 (3H, t, J = 7.3 Hz), 3.61 (2H, s), 4.14 (2H, q, J = 7.3 Hz) , 5.32 (2H, s), 7.27-7.38 (5H, m), 7.53-7.61 (2H, m), 7.95-8.00 (1H, m), 8.02-8.06 (1H, m), 8.03 (1H, d, J= 8.7Hz), 8.17 (1H, dd, J = 1.4, 8.7 Hz), 8.72 (1H, bs), 11.02 (1H, bs). Production example 439
l-Benzyl-3- (naphthalen-2-yl) -lH-pyrazolo [3, 4-b] pyrazine-5-carboxylic acid
50 mg of N- [2-benzyl-5- (naphthalen-2-yl) -4-nitroso-2if-pyrazol-3-ylamino]-maronamic acid ethyl ester obtained by Production example 438 was dissolved in 5 mL of tetrahydrofuran, added with 30 mg of a sodiummethoxide (28%) solution in methanol, and stirred at 50°C for 10 minutes. The reaction solution was partitionedbetween diluted hydrochloric acid and ethyl acetate, and the organic layer was extracted and washed with water. The organic layer was dried over magnesium sulfate, the solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=l:1), to afford 22 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, DMSO-D6) δ5.38 (2H, s) , 7.24-7.31 (3H, m) , 7.31-7.36 (2H, m), 7.50-7.56 (2H, m), 7.90-7.95 (1H, m), 7.99-8.04 (1H, m),8.02 (1H, d, J = 8.8 Hz), 8.21 (1H, bs) , 8.39 (1H, dd, J = 1.4, 8.8 Hz), 8.91 (1H, d, J = 1.4 Hz). Production example 440
l-Benzyl-3- (naphthalen-2-yl) -1H-pyrazolo [3, 4-b]pyrazine-5-carboxylic acid methyl ester
20 mg of l-benzyl-3-(naphthalen-2-yl)-1H-pyrazolo[3,4-b]pyrazine-5-carboxylic acid was dissolved in 5 mL of N,N-dimethylformamide, added with 10 mg of potassium carbonate and 20 µl methyl iodide, and stirred at room temperature for 3 hours. The reaction solution was partitioned between water and ethyl acetate, and the organic layer was extracted and washed with water. The organic layer was dried over magnesium sulfate, the solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=l:10), to afford 20 mg the title compound as a colorless powder. 1H-NMR (400MHz, CDCl3)δ4.07 (3H, s), 5.65 (2H, s), 7.25-7.36 (3H, m), 7.40-7.44 (2H, m), 7.45-7.52 (2H, m) , 7.82-7.87 (1H, m) , 7.93 (1H, d, J = 8.5 Hz) , 7.96-8.01 (1H, m) , 8.27 (1H, s) , 8.48 (1H, dd, J = 1.5, 8.5 Hz), 8.97 (1H, s) . Example 441
3-Naphthalen-2-yl-2H-pyrazolo [3, 4-b]pyrazine-5-carboxylic acid
By treating 150 mg of l-benzyl-3-(naphthalen-2-yl)-1H-pyrazolo [3, 4-b]pyrazine-5-carboxylic acid methyl ester obtained by Production example 440 in the similar method as described in Example 437 , 54 mg of the title compound was obtained as a pale brown powder.
1H-NMR (400 MHz, CD3OD) δ7 . 52-7 . 58 (2H, m) , 7.88-7.93 (1H, m) , 7.93-7.99 (1H, m) , 7.98 (1H, s) , 8.00 (1H, d, J = 9.1 Hz) , 8.24

(1H, d, J = 9.1 Hz), 8.72 (1H, bs). Example 4 42
3- (3-Fluorophenyl) -1H-pyrazolo [4, 3-b]pyridine-5-carboxylic acid obtained by Example 7 and various kinds of amine were condensed in the similar method as described in Example 44, to afford the compounds of Examples 443-446. Example 443
3- (3-Fluorophenyl) -1H-pyrazolo [4, 3-b]pyridine-5-carboxylic acid carbamoylmethyl-amide MS (ESI)m/z 314 MH+ Example 444
3- (3-Fluorophenyl) -lH-pyrazolo [4, 3-b] pyridine-5-carboxylic acid (1-carbamoyl-ethyl)-amide
1H-NMR (400 MHz, DMSO-D6) δ1.44 (3H, d, J = 7.5 Hz), 4.99 (1H, dq, J = 7.5 Hz) , 7.28 (1H, dt, J = 2.4, 7.9 Hz) , 7.27 (1H, bs) , 7.59 (1H, dt, J = 6.0, 7.9 Hz), 7.64 (1H, bs), 8.13 (1H, bd, J = 9.0 Hz), 8.25 (1H, bd, J = 9.0 Hz) , 8.2 9 (1H, dd, J = 2.4, 10.5 Hz), 8.39 (1H, bd, J=7.9Hz), 8.83 (1H, d, J = 7.5 Hz). MS (ESI)m/z 328 MH+ Example 44 5
3-(3-Fluorophenyl)-lH-pyrazolo[4,3-b]pyridine-5-carboxylic acid (l-carbamoyl-2-hydroxy-ethyl)-amide 1H-NMR (400 MHz, CD3OD)δ3.95 (1H, dd, J = 4.7, 11.2 Hz), 4.05
(1H, dd, J = 4.4, 11.2 Hz) , 4.66 (1H, bt, J = 4.7 Hz) , 7.14 (1H, dt, J = 2.6, 8.6 Hz), 7.56 (1H, dt, J = 6.0, 8.0 Hz), 8.16 (1H, d, J = 8.8 Hz) , 8.22 (1H, d, J = 8.8 Hz) , 8.23 (1H, bd, J = 10.5
Hz), 8.47 (1H, bd, J = 8.6 Hz).
MS (ESI)m/z 344 MH+
Example 446
3- (3-Fluorophenyl) -lH-pyrazolo [4, 3-b] pyridine-5-carboxylic
acid (l-carbamoyl-2-phenyl-ethyl)-amide
1H-NMR (400 MHz, DMSO-D6) δ 3.15 (1H, dd, J = 8.4, 13.9 Hz),
3.22 (1H, dd, J=5.5, 13.9Hz), 4.66 (1H, dt, J = 5.5, 8.4Hz),
7.16 (1H, bt, J=7.4Hz), 7.23 (2H, bt, J=7.4Hz), 7.23 (1H,
bs) , 7.30 (1H, dt, J = 2.8, 7.9 Hz) , 7.38 (2H, d, J = 7.4 Hz),
7.60 (1H, dt, J = 6.2, 7.9 Hz), 7.66 (1H, bs), 8.04 (1H, bd,
J= 9.0Hz), 8.20 (1H, bd, J= 9.0Hz), 8.29 (1H, bd, J = 10.5
Hz), 8.36 (1H, bd, J = 7.9 Hz), 8.63 (1H, d, J = 8.4 Hz).
MS (ESI)m/z 404 MH+
Example 4 47
3-(Naphthalen-2-yl)-lH-pyrazolo[3,4-c]pyridine-5-carboxylic acid obtained by Example 403 and various kinds of amine were condensed in the similar method as described in Example 44, to afford the compounds of Examples 448-451. Example 4 48
3-(Naphthalen-2-yl)-lH-pyrazolo[3,4-c]pyridine-5-carboxylie acid cyclopropylamide MS (ESI)m/z 329 MH+ Example 44 9
3-(Naphthalen-2-yl)-lH-pyrazolo[3,4-c]pyridine-5-carboxylic acid (furan-2-ylmethyl) amide MS (ESI)m/z 369 MH+
Example 4 50
3- (Naphthalen-2-yl)-lH-pyrazolo[3,4-c]pyridine-5~carboxylic
acid [(1S)-l-hydroxymethyl-2-methylpropyl]amide
MS (ESI)m/z 375 MH+
Example 4 51
3-(Naphthalen-2-yl)-1H-pyrazolo[3,4-c]pyridine-5-carboxylic
acid [(1S)-2-hydroxy-l-phenylethyl]amide
MS (ESI}m/z 409 MH+
Example 4 52
3-[(E)-2-(3-Fluorophenyl)-vinyl]-1H-pyrazolo[4, 3-b] pyridine-5-carboxylic acid obtained by Example 416 and various kinds of amine were condensed in the similar method as described in Example 44, to afford the compounds of Examples 453-463. Example 453
3- [ (E) -2- (3-Fluorophenyl) -vinyl] -1H-pyrazolo [4, 3-b] -pyridine-5-carboxylic acid cyclopropylamide MS (ESI)m/z 323 MH+ Example 454
3- [ {E) -2- (3-Fluorophenyl) -vinyl] ] -lH-pyrazolo [4, 3-b] -pyridine-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI)m/z 363 MH+ Example 455
3-[ (E)-2- (3-Fluorophenyl)-vinyl]-lH-pyrazolo[4, 3-b] -pyridine-5-carboxylic acid (tetrahydrofuran-2-ylmethyl)-amide MS (ESI)m/z 367 MH+
Example 456
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[4,3-b]-
pyridine-5-carboxylic acid (2-acetylamino-ethyl)-amide
MS (ESI)m/z 368 MH+
Example 4 57
3- [ (E) -2- (3-Fluorophenyl) -vinyl] -lH-pyrazolo[4, 3-b] -
pyridine-5-carboxylic acid (l-hydroxymethyl-2-methyl-
propyl)-amide
MS (ESI)m/z 369 MH+
Example 458
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[4,3-b]-
pyridine-5-carboxylic acid (2-hydroxy-l-phenyl-ethyl)-amide
MS (ESI)m/z 403 MH4
Example 459
3-[ (E) -2- (3-Fluorophenyl)-vinyl]-lH-pyrazolo [4, 3-b]-
pyridine-5-carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI)m/z 374 MH+
Example 4 60
3- [ (E) -2- (3-Fluorophenyl) -vinyl] -lH-pyrazolo [4, 3-b] -
pyridine-5-carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI)m/z 379 MH+
Example 4 61
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[4,3-b]-
pyridine-5-carboxylic acid (l-carbamoyl-2-phenyl-ethyl)-
amide
MS (ESI)m/z 430 MH+
Example 4 62
3- [ (E) -2- (3-Fluorophenyl) -vinyl] -1H-pyrazolo[4, 3-2?] -
pyridine-5-carboxylic acid (l-carbamoyl-2-hydroxy-ethyl)-
amide
MS (ESI)m/z 370 MH+
Example 4 63
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[4, 3-b]-
pyridine-5-carboxylic acid (1-carbamoyl-ethyl)-amide
MS (ESI)m/z 354 MH+
Example 4 64
3-[ (E) -2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[3, 4-c]-pyridine-5-carboxylic acid obtained by Example 433 and various kinds of amine were condensed in the similar method as described in Example 44, to afford the compounds of Examples 465-469. Example 4 65
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[3,4-c]-pyridine-5-carboxylic acid cyclopropylamide MS (ESI)m/z 323 MH+ Example 4 66
3-[(E)-2-(3-Fluorophenyl)-vinyl]]-lH-pyrazolo[3, 4-c]-pyridine-5-carboxylic acid (furan-2-ylmethyl) amide MS (ESI)m/z 363 MH+ Example 4 67
3-[(E)-2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[3,4-c]-pyridine-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methylpropyl]]amide
MS (ESI)m/z 369 MH+
Example 4 68
3- [ (E) -2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[3,4-c]-
pyridine-5-carboxylic acid [(1S)-2-hydroxy-l-
phenylethyl]amide
MS (ESI)m/z 403 MH+
Example 4 69
3-[ (E) -2-(3-Fluorophenyl)-vinyl]-lH-pyrazolo[3,4-c]-
pyridine-5-carboxylic acid [(1S)-1-carbamoylethyl]amide
MS (ESI)m/z 354 MH+
Example 47 0
3-(Naphthalen-2-yl)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid obtained by Example 405 and various kinds of amine were condensed in the similar method as described in Example 44, to afford the compounds of Examples 471-477. Example 471
3- (Naphthalen-2-yl) -2H-pyrazolo [3, 4-b] pyridine-5-carboxylic acid cyclopropylamide MS (ESI)m/z 329 MH+ Example 472
3-(Naphthalen-2-yl)-lH-pyrazolo[3,4-b]pyridine-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI)m/z 369 MH+ Example 473
3- (Naphthalen-2-yl) -1H-pyrazolo[3, 4-b]pyridine-5-carboxylic acid (tetrahydrofuran-2-ylmethyl)-amide
MS (ESI)m/z 373 MH+
Example 4 74
3- (Naphthalen-2-yl) -lH-pyrazolo [3, 4-b]pyridine-5-carboxylic
acid (2-acetylamino-ethyl)-amide
MS (ESI)m/z 374 MH+
Example 4 75
3- (Naphthalen-2-yl) -1H-pyrazolo [3, 4-b] pyridine-5-carboxylic
acid (pyridin-3-ylmethyl)-amide
MS (ESI)m/z 380 MH+
Example 47 6
3- (Naphthalen-2-yl) -lH-pyrazolo [3, 4-b] pyridine-5-carboxylic
acid (l-hydroxymethyl-2-methyl-propyl)-amide
MS (ESI)m/z 375 MH+
Example 4 77
3- (Naphthalen-2-yl) -2H-pyrazolo [3, 4-b]pyridine-5-carboxylic
acid (thiophen-2-ylmethyl)-amide
MS (ESI)m/z 385 MH+
Example 4 78
Furan-2-carboxylic acid [3-(naphthalen-2-yl)-1H-
pyrazolo [4, 3-b]pyridin-5-yl] amide
20 mg of 3-(naphthalen-2-yl)-l-trityl-1H-pyrazolo [4, 3-b]pyridin-5-ylamine obtained by Production example 422 and furan-2-carboxylic acid chloride were allowed to react in the similar method as described in Production example 423, followed by deprotection in the similar method as described in Example 16, to afford 10.3 mg of the title compound as pale
brown crystals.
1H-NMR (400 MHz, CD3OD) δ 6.69 (1H, dd, J = 1.9, 3.5 Hz), 7.39 (1H, dd, J= 0.8, 3.5 Hz), 7.46-7.54 (2H, m), 7.81 (1H, dd, J = 0.8, 1.9 Hz), 7.86-7.90 (1H, m), 7.95 (1H, d, J = 8.5 Hz), 8.02-8.05 (1H, m), 8.05 (1H, d, J = 9.5 Hz), 8.34 (1H, d, J = 9.5 Hz), 8.47 (1H, dd, J = 1.5, 8.5 Hz), 9.10 (1H, s). Example 479
N-[3-(Naphthalen-2-yl)-1H-pyrazolo[4,3-b]pyridin-5-yl]acetamide
From 20 mg of 3-(naphthalen-2-yl)-l-trityl-1H-pyrazolo [4, 3-b] pyridin-5-ylamine and acetyl chloride, 9.4 mg of the title compound was obtained as colorless crystals in the similar method as described in Example 478. 1H-NMR (400MHz, CD3OD)δ2.24 (3H, bs), 7.45-7.53 (2H, m), 7.85-7.89 (1H, m) , 7.93 (1H, d, J = 8.7 Hz), 7.96-8.00 (1H, m) , 7.98 (1H, d, J = 9.1 Hz), 8.27 (1H, bd, J = 9.1 Hz) , 8.4 8 (1H, dd, J = 1.6, 8.7 Hz), 9.10 (1H, s). Example 480
N- [3- (Naphthalen-2-yl) -lH-pyrazolo [4 , 3-b] pyridin-5-yl] -2-(thiophen-2-yl)acetamide
From 20 mg of 3-(naphthalen-2-yl)-1-trityl-lH-pyrazolo [4, 3-b]pyridin-5-ylamine and 2-thiopheneacetic acid chloride, 11. 6 mg of the title compound was obtained as colorless crystals in the similar method as described in Example 478. 1H-NMR (400 MHz, CD3OD) δ4.06 (2H, s) , 6.99 (1H, dd, J = 3.6, 5.1 Hz) , 7.07 (1H, dd, J = 1.2, 3.6 Hz) , 7.31 (1H, dd, J = 1.2, 5.1

Hz), 7.46-7.54 (2H, m) , 7.86-7.90 (1H, m) , 7.94 (1H, d, J=8.5 Hz), 7.97-8.03 (1H, m) , 7.99 (1H, d, J = 9.1 Hz), 8.28 (1H, d, J = 9.1 Hz), 8.49 (1H, dd, J = 1.7, 8.5 Hz), 9.11 (1H, s). Example 481 3- (Naphthalen-2-yl) -1H-pyrazolo [4, 3-2?] pyridin-5-ylamine
15 mg of 3-(naphthalen-2-yl)-1-trityl-lH-pyrazolo [4, 3-2?]pyridin-5-ylamine obtained by Production example 422 was deprotected in the similar method as described in Example 16, to afford 7.1 mg of the title compound as pale brown crystals.
1H-NMR (400MHz, CD3OD)δ6.79 (1H, d, J=9.1Hz), 7.44-7.52 (2H, m), 7.74 (1H, d, J = 9.1 Hz), 7.84-7.88 (1H, m), 7.93 (1H, d, J = 8.7 Hz), 7.93-7.97 (1H, m), 8.32 (1H, d, J = 8.7 Hz), 8.86 (1H, bs).
Production example 4 82
3-[(E)-2-(3-Fluorophenyl)-vinyl]-1-trityl-lH-pyrazolo [4, 3-2?]pyridine
1.95 g of 3-iodo-l-trityl-1H-pyrazolo [4, 3-2?] pyridine obtained by Production example 418 and 1.0 g of 2-[(E)-2-(3-fluorophenyl)-vinyl]-4,4,5,5-
tetramethyl [1,3,2] dioxaborolane obtained by Production example 137 were allowed to react in the manner as described in Example 194, to afford 730 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 6.62 (1H, dd, J = 1.0, 9.0 Hz), 6.92 (1H, dd, J = 4.5, 9.0 Hz) , 7.04 (2H, t, J = 8.7 Hz) , 7.21-7.32 (15H, m) , 7.38 (1H, d, J = 16.7 Hz) , 7.57 (2H, dd, J = 5.2, 8.7
Hz) , 8.15 (1H, d, J = 16.7 Hz) , 8.53 (1H, dd, J = 1.0, 4.5 Hz) .
Production example 483
3- [ (E) -2- (3-Fluorophenyl) -vinyl] -1-trityl-2H-
pyrazolo [4, 3-b]pyridine-4-oxide
470 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-1H-pyrazolo [4, 3-b] pyridine obtained by Production example 4 82 and 216 mg of m-chloroperbenzoic acid were allowed to react in the manner as described in Production example 4, to afford 210 mg of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 6.21 (1H, d, J = 8.7 Hz), 6.76 (1H, dd, J = 6.0, 8.7 Hz) , 7.02 (2H, t, J = 8.8 Hz) , 7.16-7.23 (6H, m) , 7.28-7.35 (9H, m) , 7.4 6 (1H, d, J = 16.5 Hz) , 7.53 (2H, dd, J = 5.6, 8.8 Hz), 8.03 (1H, d, J = 16.5 Hz), 8.06 (1H, dd, J =6.0 Hz). Example 4 84
5-Chloro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-pyrazolo[4,3-b]pyridine
210 mg of 3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-pyrazolo [4, 3-b]pyridine-4-oxide obtained by Production example 483 was dissolved in 5 mL of toluene, added with 240 ul of phosphorus oxychloride at room temperature and stirred for 4 days . The reaction solution was added with aqueous sodium hydrogen carbonate, extracted with ethyl acetate and washedwith water, and dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (n-hexane:ethyl acetate =3:1), to afford 75 mg
of the title compound as a colorless powder.
1H-NMR (400 MHz, CDCl3) δ 7.08 (2H, t, J = 8.7 Hz), 7.36 (1H,
d, J = 8.9 Hz), 7.37 (1H, d, J = 16.4 Hz), 7.62 (2H, dd, J =
6.0, 8.7 Hz), 7.80 (1H, d, J = 8.9 Hz), 8.10 (1H, d, J = 16.4
Hz) .
Production example 485
3-[(E)-2-(3-Fluorophenyl)-vinyl]-1-trityl-lH-
pyrazolo [4, 3-b] pyridin-5-ylamine
60 mg of 5-chloro-3-[(2E)-2-(3-fluorophenyl)vinyl]-1-trityl-lH-pyrazolo [4, 3-b] pyridine which is obtained by tritylating 5-chloro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-pyrazolo [4, 3-b]pyridine obtained by Example 484 in accordance with Production example 22 was treated with benzophenoneimine in the similar method as described in Production example 422, to afford 40 mg of the title compound as a pale yellow oil. 1H-NMR (400 MHz, CDCl3) δ 4.53 (2H, bs), 6.21 (1H, d, J = 9.0 Hz), 6.40 (1H, d, J=9.0Hz), 7.02 (2H, t, J=8.6Hz), 7.19-7.25 (6H, m), 7.25-7.32 (10H, m), 7.53 (2H, dd, J = 5.0, 8.6 Hz), 7.98 (1H, d, J = 16.5 Hz). Production example 486
3- (3-Fluorophenyl) -1-trityl-lH-pyrazolo [4, 3-b] pyridin-5-ylamine
1.1 g of 3-(3-fluorophenyl)-5-chloro-l-trityl-lH-pyrazolo [4, 3-b] pyridine obtained by Production example 37 was treatedwithbenzophenoneimine in the similarmethodas described in Production example 422, to afford 690 mg of the title compound
as pale yellow crystals.
1H-NMR (400 MHz, CDCl3) δ 4.40-4.65 (2H, bs) , 6.22 (1H, d, J = 9.0 Hz) , 6.4 0 (1H, d, J = 9.0 Hz), 7.18-7.25 (6H, m) , 7.26-7.32 (9H, m) , 7.35 (1H, dt, J = 6.2, 8.2 Hz) , 8.15 (1H, bd, J = 11.2 Hz), 8.18 (1H, d, J = 8.2 Hz). Example 4 87 3-(3-Fluorophenyl)-lH-pyrazolo[4,3-b]pyridin-5-ylamine
3- (3-Fluorophenyl) -1-trityl-1H-pyrazolo [4, 3-b]pyridin-5-ylamine was treated with trif luoroacetic acid in the similar method as described in Example 16 and so on, followed by purification by LC-MS, to give the title compound. MS (ESI) m/z 229 MH+ Example 4 88
Each 25 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [4, 3-B] pyridin-5-ylamine obtained by Production example 486 was put into several test tubes, added with 0.5 mL of tetrahydrofuran, further added with 0 . 07mmol of various kinds of acid chloride and 0.07 mmol of triethylamine, and left at room temperature all day and night. The reaction solution was added with 2 mL of water, extracted with 4 mL of ethyl acetate, and the solvent was distilled of by blowing nitrogen thereto. The resultant residue was added with 0. 5 mL of 70% trif luoroacetic acid solution in dichloromethane, sonicated at room temperature for 15 minutes, and the solvent was distilled off by blowing nitrogen thereto. The residue was dissolved in 0.25 mL of N,N-dimethylformamide, and purified by LC-MS, to afford the
compounds of Examples 4 8 9-4 92. Example 489
Cyclopropane carboxylic acid [3-(3-fluorophenyl)-1H-pyrazolo [4, 3-b]pyridin-5-yl] -amide MS (ESI) m/z 297 MH+ Example 4 90
Furan-2-carboxylic acid [3-(3-fluorophenyl)-1H-pyrazolo [4, 3-b]pyridin-5-yl] -amide MS (ESI) m/z 323 MH+ Example 4 91
N- [3-(3-Fluorophenyl)-1H-pyrazolo[4,3-b]pyridin-5-yl]-acetamide
MS (ESI) m/z 271 MH+ Example 4 92
N- [3- (3-Fluorophenyl) -1H-pyrazolo [4, 3-b] pyridine-5-yl] -2-(thiophen-2-yl)-acetamide MS (ESI) m/z 353 MH+ Example 4 93
3-[(E)-2-(3-Fluorophenyl)vinyl]-1-trityl-lH-pyrazolo [4, 3-b]pyridin-5-ylamine obtained by Production example 485 was reacted with various kinds of acid chloride in the similar method as described in Example 488, followed by deprotection and LC-MS purification, to afford the compounds of Examples 494-497. Example 4 94 Cyclopropane carboxylic acid {3-[(E)-2-(4-fluorophenyl)-
vinyl] -lH-pyrazolo [4, 3-b]pyridin-5-yl} -amide
MS (ESI) m/z 323 MH+
Example 4 95
Furan-2-carboxylic acid {3-[(E)-2-(4-fluorophenyl)-
vinyl] -1H-pyrazolo [4, 3-b]pyridin-5-yl} -amide
MS (ESI) m/z 349 MH+
Example 4 96
N-{3- [ (E)-2-(4-Fluorophenyl)-vinyl]-1H-
pyrazolo [4, 3-b] pyridin-5-yl} -acetamide
MS (ESI) m/z 297 MH+
Example 4 97
N-{3-[(E)-2-(4-Fluorophenyl)-vinyl]-1H-
pyrazolo [4, 3-b]pyridin-5-yl}-2- (thiophen-2-yl) -acetamide
MS (ESI) m/z 379 MH+
Production example 4 98
3- (3-Fluorophenyl) -1-trityl-lH-pyrazolo [4, 3-2?] pyridine-5-
carbonitrile
13.3 g of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [4, 3 -b] pyridine- 4 -oxide obtained by Production example 36 was cyanized in accordance with Production example 5, to afford 11.9 g of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ6. 90 (1H, d, J =9.0 Hz), 7.20-7.25 (6H, m) , 7.31 (1H, dt, J = 2.8, 8.6 Hz) , 7.34-7.43 (9H, m) , 7.61 (1H, dt, J = 6.0, 8.0 Hz), 7.77 (1H, d, J= 9.0Hz), 8.03 (1H, ddd, J = 1.6, 2.8, 10.4 Hz), 8.16 (1H, d, J = 8.0 Hz). Production example 4 99
Ethyl 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[4, 3-b]pyridine-5-carboxyimidate
Under nitrogen atmosphere, 100 mg of metal sodium was added to 10 mL of dry methanol, and upon complete consumption of the metal, 480 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [4, 3-b]pyridine-5-carbonitrile obtained by Production example 498 and 5 mL of dry tetrahydrofuran were added and stirred at room temperature for 2 days. The product was collected by filtration, and washed with diethyl ether, to afford 440 mg of the title compound as a colorless powder.
1H-NMR (400MHz, DMSO-D6) δ 3.89 (3H, s), 6.88 (1H, d, J = 8.8 Hz) , 7.18-7.23 (6H, m) , 7.26 (1H, dt, J= 3.0, 8.0 Hz) , 7.32-7.4 0 (9H, m), 7.58 (1H, dt, J = 5.5, 8.0 Hz), 7.63 (1H, d, J = 8.8 Hz) , 8.08 (1H, bd, J = 10.5 Hz) , 8.2 9 (1H, bd, J = 8.0 Hz) , 9.27 (1H, s).
Production example 500
3- (3-Fluorophenyl) -5- (5-methyl-2H- [1, 2, 4] triazol-3-yl) -1-trityl-lH-pyrazolo [4, 3-b]pyridine
100 mg of ethyl[3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [4 ,3-b] pyridine-5-carboxyimidate obtained by Production example 499 and 44 mg of acetohydrazide were dissolved in 3mLof pyridine, andheatedat 130°C for 2 . 5 days . Thereaction solution was added with silica gel, the solvent was evaporated, and the resultant residue was purified by silica gel column chromatography(ethyl acetate :hexane=4:6), to afford 60 mg of the title compound as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 2.50 (3H, s), 6.72 (1H, d, J = 8.7 Hz) , 7.02 (1H, dt, J = 2.9, 8.1 Hz) , 7.18-7.25 (6H, m) , 7.27-7.32 (9H, m) , 7.37 (1H, dt, J = 5.8, 8.1 Hz), 7.80 (1H, d, J = 8.7 Hz), 8.13 (1H, bd, J = 10.4 Hz), 8.23 (1H, bd, J = 8.1 Hz). Example 501
3-(3-Fluorophenyl)-5-(5-methyl-2H-[1,2,4]triazol-3-yl)-1H-pyrazolo [4, 3-b]pyridine
30 mg of 3-(3-fluorophenyl)-5-(5-methyl-lH-[1,2,4]triazol-3-yl)-l-trityl-lH-pyrazolo[4,3-b]pyridine was deprotected in the similar method as described in Production example 16, and the resultant residue was purified by LC-MS, to afford 16 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 2.46 (3H, s), 7.24 (1H, bt, J = 8.4 Hz), 7.57 (1H, bdt, J= 6.7, 8.4 Hz), 8.13 (1H, d, J = 8.7 Hz), 8.19 (1H, d, J = 8.7 Hz), 8.4 0 (1H, bd, J = 10.4 Hz), 8.51 (1H, bd, J = 8.4 Hz), 13.70 (1H, bs), 13.80-14.00 (1H, bs). MS (ESI) m/z 295 MH+ Production example 502
{5-[3-(3-Fluorophenyl)-1-trityl-lH-pyrazolo[4,3-b]pyridin-5-yl]-2H-[1,2,4]triazol-3-ylmethyl}methyl carbamic acid tert-butyl ester
100 mg of ethyl[3-(3-fluorophenyl)-1-trityl-lH-pyrazolo [4,3-b] pyridine-5-carboxyimidate obtained by Production example 499 and 80 mg of hydrazinocarbonylmethyl-methylcarbamic acid tert-butyl ester produced by Production example 367 were dissolved in a mixed solvent of 3 mL methanol-3
mL pyridine, and heated at 110°C for 3 days. The reaction solution was added with silica gel, the solvent was evaporated, and the residue was purified by silica gel column chromatography (n-hexane:ethyl acetate =2:1), to afford 74 mg of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 1.50 (9H, s) , 2.97 (3H, s), 4.60 (2H,
bs) , 6.73 (1H, bd, J = 8.8 Hz) , 7.06 (1H, dt, J= 7. 6 Hz) , 7.20-7.28
(6H, m) , 7.28-7.35 (9H, m) , 7.42 (1H, dt, J = 5.8, 7.6Hz), 7.84
(1H, bd, J = 8.8 Hz), 8.17 (1H, d, J = 10.8 Hz), 8.22-8.32 (1H,
m) .
Example 503
{5- [3-(3-Fluorophenyl)-lH-pyrazolo[4,3-2?]pyridin-5-yl]-1H-[1,2,4]triazol-3-ylmethyl}-methylamine
30 mg of {5-[3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[4, 3-b] pyridin-5-yl] -2H- [1, 2, 4] triazol-3-ylmethyl}methylcarbamic acid tert-butyl ester was deprotected in the similar method as described in Production example 16, and the resultant residue was purified by LC-MS, to obtain 15 mg of the title compound as a colorless powder. MS (ESI) m/z 324 MH+ Production example 504 (2-Chlorothiophen-3-yl)methanol
To a solution of 3.0 g thiophene-2-methanol in 26 mL of dimethylf ormamide was added 3 . 5 g of N-chlorosuccinimide at room temperature, and stirred at this temperature for a day. Adding 10 mL of 10% sodium thiosulfate, and the resultant solution was
diluted with ethyl acetate. The organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, to afford 3.0 g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 4.64 (2H, d, J = 6.0 Hz), 7.01 (1H, d, J = 6.0 Hz), 7.09 (1H, d, J = 6.0 Hz). Production example 505 (5-Bromo-2-chlorothiophen-3-yl)methanol
To a solution of 3.0 g of (2-chlorothiophen-3-yl)methanol in 60 mL of dimethylformamide was added 3.59 g of N-bromosuccinimide at room temperature, and stirred at this temperature for 2 hours . Adding 10 mL of 10% sodium thiosulf ate, and the resultant solution was diluted with ethyl acetate. The organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, to afford 3.7 g of the title compound as a colorless oil. 1H-NMR (400 MHz, CDCl3) δ 4.58 (2H, d, J = 6.0 Hz), 7.01 (1H, s).
Production example 506 5-Chloro-4-hydroxymethylthiophene-2-carbonitrile
To a solution of 25.0 g (5-bromo-2-chlorothiophen-3-yl)methanol in 330 mL of dimethylformamide, 25.8 g of zinc cyanide (I) and 12.7 g tetrakis(triphenylphosphine)palladium(O) were added at room
temperature, and stirred at 100°C for 4 hours. Diluting with ethyl acetate, the organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:10-1:5) , to afford 16.0 g of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ 4.65 (2H, s) , 7.57 (1H, s) . Production example 507 5-Chloro-4-formylthiophene-2-carbonitrile
To a solution of 3.0 g of 5-chloro-4-hydroxymethylthiophene-2-carbonitrile in 34 mL of dichloromethane was added 8.06 g of Dess-Martin reagent at room temperature, and stirred at this temperature for a day. After diluting with dichloromethane, washing successively with aqueous sodium hydrogen carbonate and saturated brine, and drying over anhydrous magnesium sulfate, the solvent was evaporated, to afford 4.5 g of a crude product of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 7.86 (1H, s) , 10.00 (1H, s). Production example 508
Acetic acid(2-chloro-5-cyanothiophen-3-ylmethylene)hydrazide
To a suspension of 4.5 g of 5-chloro-4-formylthiophene-2-carbonitrile in ethanol was added 2.56 g of acetylhydrazide at room temperature, and heated at reflux for
2 hours. After cooling to room temperature, the crystals were
collected by filtration, washed with ethanol, to afford 2.53
g of the title compound (diastereomic mixture of E form:Z
form=5:2) as pale yellow crystals.
(E) compound: 1H-NMR (400 MHz, DMSO-D6) δ 2.18 (3H, s), 7.97 (1H,
s), 8.22 (1H, s), 11.39 (1H, bs).
(Z) compound:1H-NMR (400 MHz, DMSO-D6) δ 1.95 (3H, s) , 8.09 (1H,
s), 8.20 (1H, s), 11.55 (1H, bs).
Production example 509
1-Acetyl-lH-thieno[2,3-c]pyrazole-5-carbonitrile
To a solution of 2.53 g of acetic acid (2-chloro-5-cyanothiophen-3-ylmethylene)hydrazide in 22 mL of diphenyl ether were added copper powder and potassium acetate at room temperature, and stirred at 200 °C for 6 hours . Following filtration through Celite, the filtrate was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (ethyl
acetate :n-hexane=l:10-1:5), to afford 215 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ 2.72 (3H, s) , 8.17 (1H, s) , 8.40 (1H, s).
Production example 510 lH-Thieno[2, 3-c]pyrazole-5-carbonitrile
To a suspension of 215 mg of
1-acetyl-1H-thieno[2, 3-c]pyrazole-5-carbonitrile in 8 mL of
ethanol, 1 mL of 5N sodium hydroxide aqueous solution was added
at room temperature, and stirred at this temperature for 10
minutes. Then the solution was neutralized with 5N hydrochloric
acid, extracted with ethyl acetate, and the organic layer was
washed with saturated brine, dried over anhydrous magnesium
sulfate, and the solvent was evaporated, to afford 168 mg of
the title compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ 8.07 (1H, s) , 8.34 (1H, s).
Production example 511
3-Bromo-lH-thieno[2,3-c]pyrazole-5-carbonitrile
To a solution of 168 mg of lH-thieno[2,3-c]pyrazole 5-carbonitrile in 5 mL of dimethylformamide was added 200 mg of N-bromosuccinimide at room temperature and stirred at this temperature for 2 hours. After adding 1 mL of 10% sodium thiosulfate aqueous solution, the solution was diluted with ethyl acetate. The organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. This afforded 3.7 g of the title compound as a colorless oil. The crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:10), to afford 220 mg of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ 8.00 (1H, s) . Example 512 5-Iodo-3-(naphthalen-2-yl)-1H-thieno[2,3-c]pyrazole
To a solution of 70 mg of 3-naphthalene-2-yl-lH-thieno[2,3-c]pyrazole obtained by Example 78 in 3.0 mL of N,N-dimethylformamide was added 63 mg of N-iodosuccinimide at room temperature, and stirred at 40°C for a day. Then the solution was added with 1. 0 mL of 10% sodium thiosulf ate aqueous solution, and extracted with ethyl acetate . The organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:3), to afford 75 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ 7.49-7.57 (2H, m) , 7.90-7.94 (1H, m), 7.98-8.07 (3H, m), 8.01 (1H, s), 8.43 (1H, s). Production example 513
5-Iodo-3-(naphthalen-2-yl)-1-trityl-lH-thieno[2,3-c] -pyrazole
To a solution of 75 mg of 5-iodo-3-(naphthalen-2-yl)-lH-thieno[2,3-c]pyrazole in 1 m L of N,N-dimethylformamide 1 was added 12 mg of sodium hydride at room temperature, stirred for 15 minutes, then added with 56 mg of trityl chloride, and stirred at this temperature for a day. The solution was added with water and diluted with ethyl acetate, and the organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated.
The residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:30), to afford 93 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 7.25-7.38 (15H, m), 7.43-7.50 (2H, m) , 7.57 (1H, s), 7.80-7.89 (3H, m) , 8.03 (1H, dd, J= 8.4, 2.0 Hz), 8.25 (1H, d, J - 0.8 Hz). Example 514
3-(Naphthalen-2-yl)-1H-thieno[2,3-c]pyrazole-5-carbonitrile To a solution of a crude product which was obtained by treating 93 mg of 5-iodo-3-(naphthalen-2-yl)-1-trityl-1H-thieno [2 ,3-c] pyrazole obtained by Production example 513 in the manner as described in Production example 506 (purification by silica gel column is not executed) in 2 mL of dichloromethane, 1 mL of trif luoroacetic acid was added at room temperature and stirred at this temperature for 30 minutes. The reaction solution was poured into aqueous sodium hydrogen carbonate, extracted with ethyl acetate, and the organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:3), to afford 33 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ 7.53-7.61 (2H, m) , 7.94-7.97 (1H, m) , 8.01-8.11 (2H, m) , 8.05 (1H, s) , 8.53 (1H, d, J = 0.8 Hz) , 8.70 (1H, s). Example 515
3-(Naphthalen-2-yl)-lH-thieno[2,3-c]pyrazole-5-carboxylic acid
To a solution of 33 mg of 3-(naphthalen-2-yl)-lH-thieno[2,3-c]pyrazole-5-carbonitrile in 1.0 mL of acetic acid and 0.3 mL of water was added 0.3 mL of concentrated sulfuric acid at room temperature and stirred at 110°C for a day. After cooling the solution to room temperature, 10 mL of ice water was added, and the precipitated crystals were collected by filtration and washed with water, to afford 33 mg of the title compound as pale brown crystals. 1H-NMR (400 MHz, DMSO-D6) δ 7.50-7.60 (2H, m) , 7.91-8.15 (4H, m) , 8.34 (1H, s), 8.54 (1H, s). Production example 516
3-(Naphthalen-2-yl)-1-trityl-lH-thieno[2,3-c]pyrazole-5-carboxylic acid
By treating 56 mg of 3-(naphthalen-2-yl)-lH-thieno[2,3-c]pyrazole-5-carboxylic acid in the manner as described in Production example 513, 61 mg of the title compound was obtained as pale brown crystals. 1H-NMR (400 MHz, DMSO-D6) δ 7.22-7.27 (6H, m) , 7.39-7.45 (9H, m) , 7.50-7.57 (2H, m) , 7.90-7.93 (1H, m) , 7.96 (1H, d, J = 9.2 Hz), 8.03 (1H, dd, J = 9.2, 1.6 Hz), 8.14-8.18 (1H, m), 8.28 (1H, s), 8.53 (1H, s). Production example 517
3-(Naphthalen-2-yl)-1-trityl-lH-thieno[2,3-c]pyrazole-5-carbonitrile
By treating 310 mg of 5-iodo-3-(naphthalen-2-yl)-1-trityl-lH-thieno[2,3-c]-pyrazole obtained by Production example 513 in the manner as described in Production example 506, 167 mg of the title compound was obtained as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 7.25-7.42 (15H, m) , 7.47-7.54 (2H, m) , 7.83-7.92 (3H, m) , 7.90 (1H, s) , 8.02 (1H, dd, J=8.4, 2.0 Hz), 8.28 (1H, d, J = 0.8 Hz). Production example 518
c-[3-(Naphthalen-2-yl)-1-trityl-lH-thieno[2,3-c]pyrazol-5-yl]methylamine
Undernitrogen atmosphere, a suspension of 51. 2mgof lithium aluminum hydride in 1.0 mL of tetrahydrofuran was added with 180 mg of aluminum chloride at 0°C, and stirred at room temperature for 20 minutes. A solution of 167 mg of 3- (naphthalen-2-yl) -1-trityl-lH-thieno[2,3-c]pyrazole-5-carbonitrile in 2.0 mL of tetrahydrofuran was added and stirred at this temperature for 3 hours. Under ice cooling, 10 mL of Rochelle salt aqueous solution was slowly added, followed by filtration through Celite. The filtrate was diluted with ethyl acetate, washed with saturated brine, dried over anhydrous magnesium sulfate, and then the solvent was evaporated. The crude product was purified and separated by NH silica gel column chromatography (ethyl acetate :n-hexane=l:1), to afford 86 mg of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ 3.91 (2H, s), 7.17 (1H, t, J = 1.2
Hz), 7.27-7.35 (15H, m) , 7.45 (2H, ttd, 6.8, 6.8, 1.6Hz), 7.81 (1H, dd, J = 8.4, 1.6 Hz), 7.84 (1H, d, J = 8.4 Hz), 7.87 (1H, dd, J=8.4, 1.6Hz), 8.07 (1H, dd, J=8.4, 1.6 Hz), 8.29 (1H, d, J = 1.2 Hz) . Example 519
3-(Naphthalen-2-yl)-l-trityl-lH-thieno[2,3-c]-pyrazol-5-carboxylic acid obtained by Production example 516 and various kinds of amine were dehydration-condensed in accordance with Example 44, deprotected in accordance with the deprotection condition of Example 183 and purified by LC-MS, to afford the compounds of Examples 520-523. Example 520
3-(Naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-carboxylic acid cyclopropylamide MS (ESI) m/z 334 MH+ Example 521
3-(Naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-carboxylic acid (furan-2-ylmethyl) amide MS (ESI) m/z 374 MH+ Example 522
3-(Naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-carboxylic acid (2-hydroxy-l-phenylethyl) amide MS (ESI) m/z 414 MH+ Example 523
3-(Naphthalen-2-yl)-lH-thieno[2,3-c]pyrazol-5-carboxylic acid (1-carbamoylethyl) amide
MS (ESI) m/z 365 MH+ Example 524
c-[3-(Naphthalen-2-yl)-1-trityl-lH-thieno[2,3-c] -pyrazol-5-yl]methylamine obtainedby Production example 518 and various kinds of carboxylic acid were amidated in accordance with Example 183, deprotected and purified by LC-MS, to afford the compounds of Examples 525-532. Example 525
N- [3-(Naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-ylmethyl]acetamide MS (ESI) m/z 322 MH+ Example 526
Cyclopropane carboxylic acid [3-(naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-ylmethyl]amide MS (ESI) m/z 348 MH+ Example 527
Thiophene-2-carboxylic acid [3-(naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-ylmethyl]amide MS (ESI) m/z 390 MH+ Example 528
Furan-2-carboxylic acid [3-(naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-ylmethyl]amide MS (ESI) m/z 374 MH+ Example 52 9
(2S)-5-Oxopyrrolidine-2-carboxylic acid [3-(naphthalen-2-yl)-lH-thieno[2,3-c]pyrazol-5-ylmethyl]amide
MS (ESI) m/z 391 MH+
Example 530
2-Methoxy-N-[3-(naphthalen-2-yl)-lH-thieno[2/3-c]pyrazol-5-
ylmethyl]benzamide
MS (ESI) m/z 414 MH+
Example 531
3-Methoxy-N- [3-(naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-
ylmethyl]benzamide
MS (ESI) m/z 414 MH+
Example 532
Pyridine-2-carboxylic acid
[3-(naphthalen-2-yl)-1H-thieno[2,3-c]pyrazol-5-
ylmethyl]amide
MS (ESI) m/z 385 MH+
Production example 533
2,2-Dimethyl-N-(pyridin-4-yl)-propionamide
To a solution of 20.0 g of 4-aminopyridine in 100 mL of dichloromethane, 32.6 mL of triethylamine was added at 0°C, followed by 27.5 mL of pivaloyl chloride, and stirred at this temperature for 1 hour. The organic layer was washed successively with water and saturatedbrine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, and the precipitated crystals were collected by filtration, and washed with a mixed solvent of diethyl ether and n-hexane. The filtrate was diluted with ethanol, and treated with 5 N sodium hydroxide at room temperature, thereby hydrolyzing coexisting dipivaloyl
compounds into monopivaloyl compounds. Following neutralization with 5N hydrochloric acid, and extraction with ethyl acetate, the organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, the precipitated crystals were collected by filtration, and washed with a mixed solvent of diethyl ether and n-hexane. This was then combined with the previously obtained crystals, to afford 25.1 g of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 1.32 (9H, s) , 7.47 (1H, bs) , 7.4 9 (2H, dd, J = 4.8, 1.6 Hz), 8.48 (2H, dd, J = 4.8, 1.6 Hz). Production example 534 2, 2-Dimethyl-iV- (3-methylpyridin-4-yl) propionamide
From 20.0 g of 2,2-dimethyl-N- (pyridin-4-yl)-propionamide, 19.9 g of the title compound was obtained as pale yellow oil in the manner as described in the document {J.Org.Chem., 1983,48,3401. ) .
1H-NMR (4 00 MHz, CDCl3) δ 1.35 (9H, s) , 7.4 0 (1H, bs) , 8.17 (1H, d, J = 5.6 Hz), 8.34 (1H, s), 8.39 (1H, d, J = 5.6 Hz). Production example 535 N- (3-Methylpyridin-4-yl)acetamide
To 17.5 g of 2,2-dimethyl-N- (3-methylpyridin-4-yl)propionamide, 70mLof 5N hydrochloric acid aqueous solution was added and stirred at 90°C for a day. Under ice cooling, the solution was neutralized with 5N sodium hydroxide aqueous solution, and the solvent was
evaporated. The crystals were washed with a mixed solution of
dichloromethane:methanol =10:1, and the solvent of filtrate was
evaporated. To a solution of the resultant crude product in
100 mL of pyridine, 17.2 mL of acetic anhydride was added at
room temperature and stirred at this temperature for 6 hours.
The solvent was evaporated, and the residue was purified and
separated by NH silica gel column chromatography (ethyl
acetate :n-hexane=l:1), to afford 12.1 g of the title compound
as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 2.25 (3H, s) , 2.25 (3H, s) , 7.10 (1H,
bs), 8.12 (1H, d, J = 5.6 Hz), 8.35 (1H, s), 8.39 (1H, d, J
=5.6 Hz).
Production example 536
1-Pyrazolo[4,3-c]pyridin-1-yl-ethanone
By treating 12.1 g of N-(3-methylpyridin-4-yl)acetamide in the manner as described in Production example 408, 6.44 g of the title compound was obtained as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ 2.81 (3H, s), 8.25 (1H, d, J = 0.8 Hz), 8.28 (1H, dt, J = 6.0, 0.8 Hz), 8.66 (1H, d, J = 6.0 Hz), 9.13 (1H, d, J = 0.8 Hz). Production example 537 lH-Pyrazolo[4,3-c]pyridine
To a solution of 6.44 g of l-pyrazolo[4,3-c]pyridin-l-yl-ethanone in 120 mL of ethanol, 10 mL of 5N sodium hydroxide aqueous solution was added at room temperature and stirred at this temperature for 30 minutes.
After neutralization with 5N hydrochloric acid, the solvent was evaporated, the residue was washed with ethyl acetate, and the solvent of filtrate was evaporated, to afford 3.88 g of the title compound as pale yellow crystals.
1H-NMR (400 MHz, CDCl3) δ 7.43 (1H, dt, J = 6.0, 0.8 Hz), 8.24 (1H, d, J = 0.8 Hz), 8.46 (1H, d, J = 6.0 Hz), 9.17 (1H, d, J = 0.8 Hz).
Production example 538 3-Bromo-lH-pyrazolo[4,3-c]pyridine
To a solution of 3.85 g of lH-pyrazolo[4, 3-c]pyridine in 50 mL of N,N-dimethylformamide was added 6.04 g of N-bromosuccinimide at room temperature and stirred at this temperature for 2 hours. Adding 10 mL of 10% sodium thiosulfate aqueous solution, the solution was diluted with ethyl acetate. The organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, to afford 4.69 g of the title compound as pale red-brown crystals. 1H-NMR (400 MHz, DMSO-D6) δ 7.58 (1H, d, J = 2.0 Hz), 8.42 (1H, d, J = 2.0 Hz), 8.95 (1H, s), 13.81 (1H, bs). Production example 539 3-Bromo-l-trityl-lH-pyrazolo[4,3-c]pyridine
To a solution of 4.69 g of 3-bromo-lH-pyrazolo[4,3-c]pyridine in 72 mL of dimethylformamide was added 1.42 g of sodium hydride at room temperature and stirred for 15 minutes, and then added with 6.6
g of trityl chloride and stirred at this temperature for a day. The solution was diluted with dichloromethane, and the organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The precipitated crystals were washed with ethyl acetate, the filtrate was evaporated, and the residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:10-1:3) , an the resultant product was combined with the above crystals, to afford 5.28 g of the title compound as colorless crystals. 1H-NMR (4 00 MHz, DMSO-D6) δ 6.22 (1H, dd, J=6.4, 1.2 Hz) , 7.13-7.40 (15H, m), 8.15 (1H, d, J= 6.4 Hz), 8.93 (1H, d, J = 1.2 Hz). Production example 540 3-(3-Fluorophenyl)-1-trityl-lH-pyrazolo[4, 3-c]pyridine
A solution of 500 mg of 3-bromo-l-trityl-1H-pyrazolo [4, 3-c]pyridine, 131 mg of 3-fluorophenylboronic acid,
tetrakis(triphenylphosphine)palladium(O) and 537 mg of barium hydroxide octahydrate in a mixed solution of 18 mL of dimethoxyethane and 3 mL of water was stirred at 80°C for 2 hours . The solution was diluted with ethyl acetate and water, followed by filtration through Celite. The organic layer of filtrate was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:10-1:3-dichloromethane:n-hexane=l:2-1:1
-dichloromethane:methanol 100:1), to afford 399 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 6.20 (1H, dt, J = 6.0, 0.8 Hz), 7.08 (1H, td, J = 8.0, 0.8 Hz), 7.17-7.32 (15H, m), 7.43 (1H, td, J = 8.0, 6.0 Hz), 7.61 (1H, ddd, J = 10.0, 2.0, 1.6 Hz), 7.73 (1H, dt, J = 8.0, 1.2 Hz), 8.05 (1H, d, 6.4 Hz), 9.35 (1H, d, J = 0.8 Hz) . Example 541 3-(3-Fluorophenyl)-lH-pyrazolo[4,3-c]pyridine
To a solution of 12 mg of 3- (3-f luorophenyl) -1-trityl-2H-pyrazolo [4, 3-c]pyridine in 2 mL of dichloromethane was added 1 mL of trifluoroacetic acid at roomtemperature and stirred at this temperature for 10 minutes . The reaction solution was poured into aqueous sodium hydrogen carbonate, extracted with ethyl acetate, and the organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crude product was purified and separatedby silica gel column chromatography (ethyl acetate : n-hexane=l: 10-1: 3) , to afford 4 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, CD3OD) δ 7.21 (1H, tdd, J= 8.0, 2.4, 0.8 Hz), 7.57 (1H, td, J=8.0, 6.0 Hz), 7.60 (1H, dd, J=6.0, 1.2 Hz), 7.75 (1H, ddd, J = 10.4, 2.8, 1.6 Hz), 7.86 (1H, dt, J = 8.0, 1.2 Hz), 8.37 (1H, d, 6.0 Hz), 9.33 (1H, d, J = 0.8 Hz). Production example 542 3-(3-Fluorophenyl)-1-trityl-lH-pyrazolo[4,3-c]pyridin-5-ol
By treating 380 mg of 3-(3-fluorophenyl)-1-trityl-1H-pyrazolo[4, 3-c]pyridine obtained by Production example 540 in the manner as described in Production example 4, 341 mg of the title compound was obtained as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 6.14 (1H, d, J = 8.0 Hz), 7.09 (1H, td, J = 8.0, 0.8 Hz) , 7.16-7.34 (15H, m) , 7.42 (1H, td, J = 8.0, 6.0Hz), 7.48 (1H, dt, J=10.0, 1.6Hz), 7.55 (1H, dd, J=8.0, 0.8 Hz), 7.7 4 (1H, dd, 8.0, 1.6 Hz), 8.96 (1H, d, J = 0.8 Hz) . Production example 543
3-(3-Fluorophenyl)-1-trityl-l,5-dihydropyrazolo[4, 3-c] -pyridin-4-one
A solution of 340 mg of 3-(3-fluorophenyl)-1-trityl-lH-pyrazolo[4,3-c]pyridine-5-ol in acetic anhydride was stirred at 80°C for a day and at 100°C for 12 hours. Excess acetic anhydride was distilled off under reduced pressure, and a solution of residue in 5 mL of ethanol was added with 1 mL of 5N sodium hydroxide aqueous solution at room temperature and stirred for 1 hour. The solution was diluted with ethyl acetate, washed successively with saturated aqueous sodium hydrogen carbonate and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The resultant crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:3-1:0), to give 195 mg of the title compound as colorless crystals, as well as 45 mg of 3-(3-fluorophenyl)-1,5-
dihydropyrazolo[4,3-c]pyridin-4-one described in Example 544
described below in which detritylation partially proceeded as
colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ 5.23 (1H, d, J =7.2 Hz), 6.92 (1H,
dd, J = 7.2, 6.0 Hz) , 7.10-7.42 (17H, m) , 8.01 (1H, dt, J = 7.6,
1.2 Hz), 8.22 (1H, ddd, J = 11.2, 2.4, 1.2 Hz), 11.19 (1H, d,
J = 6.0 Hz) .
Example 54 4
3-(3-Fluorophenyl)-1,5-dihydropyrazolo[4,3-c]pyridin-4-one
1H-NMR (400 MHz, DMSO-D6) δ 6.45 (1H, d, J=7.2Hz), 7.17 (2H,
m), 7.45 (1H, td, J = 8.0, 2.4 Hz), 8.19 (1H, d, J = 8.0 Hz),
8.37 (1H, ddd, J = 7.6, 2.4, 1.2 Hz), 11.04 (1H, d, J = 4.8Hz).
Example 54 5
3-(3-Fluorophenyl)-5-(2-methoxyethyl)-1,5-
dihydropyrazolo[4,3-c]pyridin-4-one
To a solution of 10 mg of 3-(3-fluorophenyl)-1-trityl-1,5-dihydropyrazolo[4,3-c]pyridin-4-one obtained by Production example 54 3 in 0.5 mL of dimethylformamide was added 2 mg of sodium hydride at room temperature, stirred for 5 minutes, then added with 25ul 2-bromoethylmethylether (in 1.0 M dimethylformamide) , and stirred at this temperature for a day. After adding aqueous ammonium chloride, the solution was extracted with ethyl acetate, and the organic layer was concentrated. The residue was dissolved in dichloromethane, added with 0.5 mL of trifluoroacetic acid at room temperature and stirred for 10 minutes. Following concentration,
separation and purification by LC-MS were performed, to afford
0.89 mg of the title compound as colorless crystals.
MS (ESI) m/z 288 MH+
Example 54 6
3-(3-Fluorophenyl)-4-oxo-l,4-dihydropyrazolo[4,3-c]pyridine
-5-carboxylic acid ethylamide
To a solution of 10 mg of 3- (3-fluorophenyl)-1-trityl-1,5-dihydropyrazolo[4,3-c]pyridin-4-one obtained by Production example 543 in 0. 5 mL of chloroform was added 25 ul of ethyl isocyanate (in 1.0 M chloroform) at room temperature, and stirred at the same temperature for a day. At the same temperature, 0 . 5 mL oftrifluoroaceticacidwas added and stirred for 10 minutes. Following concentration, the residue was separated and purified by LC-MS, to afford 1.57 mg of the title compound as colorless crystals. MS (ESI) m/z 301 MH+ Example 547
3-(3-Fluorophenyl)-4-oxo-l,4-dihydropyrazolo[4,3-c]pyridine -5-carboxylic acid benzylamide
From 10 mg of 3-(3-fluorophenyl)-1-trityl-l,5-dihydropyrazolo[4,3-c]pyridin-4-one obtained by Production example 543 and 25 ul of benzyl isocyanate (in 1. 0 M chloroform) , 1.16 mg of the title compound was obtained as colorless crystals in accordance with Example 54 6. MS (ESI) m/z 363 MH+ Production example 548
5-Bromo-2-chloro-4-methylpyrimidine
5 g of 5-bromo-2,4-dichloropyrimidine was dissolved in 70 mL of tetrahydrofuran, and under stirring at room temperature, 15.8 g of trimethyl aluminum in 15% n-hexane and 1.77 g of tetrakis(triphenyl)phosphine were added, and stirred at 80°C, under nitrogen atmosphere for 7 hours. Under ice cooling, 70 mL of water was added little by little, followed by 46 g of potassium sodium tartrate tetrahydrate, and stirred at room temperature for 1 hour. Then the solution was extracted twice with ethyl acetate, and the resultant organic layer was washed with saturated brine. After drying over magnesium sulfate, the solvent was evaporated, and purification and isolation by silica gel column chromatography (ethyl acetate :n-hexane=l:8) were followed, to obtain 1. 6 g of the title compound as a pale yellow syrup.
1H-NMR (400 MHz, CDCl3) δ 2.63 (3H, s), 8.57 (1H, s) Production example 54 9 2-Chloro-4-methylpyridin-5-ylamine
308 mg of 5-bromo-2-chloro-4-methylpyridine was dissolved in 6 mL of toluene, added with 680 mg cesium carbonate, 0. 3 mL of benzophenoneimine, 42 mg of 2, 2'-bis(diphenylphosphino)-1,1'-binaphtyl and 46 mg of tris (dibenzylideneacetone)bis palladium, and stirred under nitrogen atmosphere at 110°C for 15 hours. The reaction solution was allowed to cool to room temperature, then added with saturated brine, extracted with ethyl acetate, dried over magnesium sulfate, and then the solvent
was evaporated. The residue was added with 10 mL of tetrahydrofuran and 10 mL of 5N hydrochloric acid, and stirred at room temperature for 1 hour. The reaction solution was added with aqueous sodium hydrogen carbonate, extracted with ethyl acetate, and the organic layer was washed with saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate :hexane=l:1), to afford 56 mg of the title compound.
1H-NMR (400 MHz, CDCl3) δ 2.40 (3H, s), 3.67 (2H, brs), 7.96 (1H, s)
Production example 550 N-(2-Chloro-4-methylpyrimidin-5-yl)-acetamide
1.36 g of 2-chloro-4-methylpyrimidin-5-ylamine was dissolved in 20 mL of dichloromethane, and under stirring on ice, 1.53 mL of pyridine, 2.7 mL of acetic anhydride and 1.16 g of 4-dimethylaminopyridine were added. After stirring at room temperature for 1 hour, water was added, extracted with ethyl acetate, and the resultant organic layer was washed with saturated brine and dried over magnesium sulfate. After evaporating the solvent, the residue was purified by silica gel column chromatography (ethyl acetate :hexane=2:1), to afford 178 mg of the title compound as a brown oil.
1H-NMR (400 MHz, CDCl3) δ 2.26 (3H, s) , 2.50 (3H, s) , 7.07 (1H, brs), 9.01 (1H, s) Production example 551
1-(5-Chloro-pyrazolo[4,3-d]pyrimidin-l-yl)-ethanone From 110 mg of N-(2-chloro-4-methylpyrimidin-5-yl) -acetamide, 27 mg of the title compound was obtained in the manner as described in Production example 408. 1H-NMR (400 MHz, CDCl3) δ 2.83 (3H, s) , 8.33 (1H, s) , 9.71 (1H, s)
Production example 552 5-Chloro-lH-pyrazolo [4 , 3-d] pyrimidine
27 mg of 1-(5-chloro-pyrazolo[4,3-d]pyrimidin-1-yl) -ethanone was dissolved in 1. 5 mL of acetonitrile, added with 1.5 mL of 5N sodium hydroxide aqueous solution, and stirred for 2 hours. After adding 2N hydrochloric acid to the reaction solution to render it acidic, the solution was extracted with ethyl acetate, and the resultant organic layer was washed with saturated brine, then dried over magnesium sulfate, and the solvent was evaporated, to afford 16 mg of the title compound. 1H-NMR (400 MHz, CDCl3) δ 8.32 (1H, s) , 9.14 (1H, s) Production example 553 5-Chloro-3-iodo-l-trityl-lH-pyrazolo[4,3-d]pyrimidine
From 58 mg of 5-chloro-1H-pyrazolo[4,3-d]pyrimidine, 160 mg of the title compound was obtained in the manner as described in Production example 206, followed by the manner as described in Production example 22.
1H-NMR (400 MHz, CDCl3) δ 7.11-7.36 (16H, m) Production example 554 5-Chloro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-
trityl-lH-pyrazolo[4,3-d]pyrimidine
From 43 mg of 5-chloro-3-iodo-l-trityl-lH-
pyrazolo[4,3-d]pyrimidine, 29 mg of the title compound was
obtained in accordance with Production example 181.
1H-NMR (400 MHz, CDCl3) δ 7.05 (2H, t, J = 8.8 Hz), 7.10-7.37
(17H, m) , 7.52-7.58 (2H, m), 8.01 (1H, d, J = 16.8 Hz)
Example 555
5-Chloro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
pyrazolo[4,3-d]pyrimidine
From 15 mg of 5-chloro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-1-trityl-lH-pyrazolo[4,3-d]pyrimidine
obtained by Production example 554 , 0 . 61 mg of the title compound
was obtained in accordance with Production example 16.
1H-NMR (400 MHz, CD3OD) δ 7.14 (2H, t, J = 8.8 Hz), 7.39 (1H,
d, J = 16.8 Hz), 7.66 (2H, dd, J = 8.8, 5.2 Hz), 8.04 (1H, d,
J = 16.8 Hz), 9.13 (1H, s)
Production example 556
6-Fluoro-3-iodo-1H-indazole-5-carbonitrile
From 6.01 g of 6-fluoro-lH-indazole-5-carbonitrile
obtained by Production example 82, 11.6 g of the title compound
was obtained as pale brown crystal in accordance with the method
of Production example 206.
1H-NMR (400 MHz, CDCl3) δ 7.30 (1H, d, J = 8.8 Hz), 7.88 (1H,
d, J = 8.8 Hz), 10.8 (1H, brs)
Production example 557
6-Fluoro-3-iodo-l-trityl-lH-indazole-5-carbonitrile
From 11.6 g of 6-fluoro-3-iodo-lH-indazole-5-carbonitrile obtained by Production example 556, 14 . 9 g of the title compound was obtained as pale brown crystal in accordance with the method of Production example 22.
1H-NMR (400 MHz, CDCl3) δ 6.07 (1H, d, J =10.4 Hz), 7.10-7.17 (6H, m) , 7.25-7.33 (9H, m) , 7.78 (1H, d, J = 6.0 Hz) Example 558
6-Fluoro-3-{ (E) -2-(4-fluorophenyl)-vinyl}-lH-indazole-5-carbonitrile
By treating 4 gof 6-fluoro-3-iodo-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 557 in the similar method as described in Example 100, 1.37 g of the title compound was obtained as pale brown crystals.
1H-NMR (400 MHz, DMSO-D6) δ 7.24 (2H, t, J =8.8 Hz), 7.49 (1H, d, J = 16.8 Hz), 7.62 (1H, d, J = 16.8 Hz), 7.63 (1H, d, J = 9.6 Hz), 7.79 (2H, dd, J = 8.8, 5.6 Hz), 8.95 (1H, d, J = 6.0 Hz)
Example 559
6-Fluoro-3-{(E)-2-(4-fluorophenyl)-vinyl}-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride
From 1.37 of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carbonitrile obtained by Example 558, 1.78 g of the title compound was obtained as pale yellow crystals in accordance with Example 370. 1H-NMR (400 MHz, DMSO-D6) δ 1.49 (3H, t, J = 7.2 Hz) 4.66 (2H, q, J = 7.2 Hz) 7.25 (2H, t, J = 8.8 Hz), 7.54 (1H, d, J = 16.8
Hz), 7.62 (1H, d, J=9.6Hz), 7.68 (1H, d, J=16.8Hz), 7.77-7.84 (2H, m), 8.85 (1H, d, J = 6.4 Hz) Production example 560 N-Formyl-N-methylhydrazine
5 g of Methylhydrazine was dissolved in 50 mL of tetrahydrofuran, and under stirring at room temperature, 8.8 mL of ethyl formate was added and stirred for 14 hours. By evaporating the solvent, 8.42 g of a crudely purified product of the title compound as a pale yellow syrup. 1H-NMR (400 MHz, DMSO-D6) δ 2.91 (3H, s), 4.80 (2H, brs), 8.01 (1H, s)
Production example 561 N-Acetyl-N-methylhydrazine
5.2 g of methylhydrazine was dissolved in 50 mL of tetrahydrofuran, and under stirring on ice, 10.6 mL of acetic anhydride was added and stirred at room temperature for 7 hours. By distilling off the solvent under reduced pressure, 17.8 g of a crudely purified product of the title compound as a colorless syrup.
1H-NMR (400 MHz, DMSO-D6) δ 2.01 (3H, s), 2.97 (3H, s), 4.71 (2H, brs) Example 562
In accordance with the method described in Example 371 or 375, from 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 and 6-fluoro-3-{(E)-2-
(4-fluorophenyl)-vinyl}~lH-indazole-5-carboxymidic acid
ethyl ester hydrochloride obtained by Example 559, the compounds
of Examples 563-598 were produced. Hydrazines used in Examples
563-591, 597, 598 were produced in accordance with Production
examples 366-367 unless they were commercially available. In
Examples 592 and 594, N-formyl-N-methylhydrazine produced by
Production example 560 was used, and in Example 593
N-acetyl-N-methylhydrazine produced by Production example 561
was used.
Example 563
1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-
5-yl}-4H-[l,2,4]triazol-3-yl)-ethanol
MS (ESI) m/z 368 MH+
Example 564
3-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-
5-yl}-4H-[l,2,4]triazol-3-yl)-propan-l-ol
MS (ESI) m/z 382 MH+
Example 565
1-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-
5-yl}-4H-[l,2,4]triazol-3-yl)-ethanol
MS (ESI) m/z 368 MH+
Example 566
3-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-
5-yl}-4H-[l,2,4]triazol-3-yl)-propan-l-ol
MS (ESI) m/z 382 MH+
Example 567
6-Fluoro-3-[(E)-2-{4-fluorophenyl)-vinyl]-5-(4H- [1, 2, 4] triazol-3-yl) -1H-indazole MS (ESI) m/z 324 MH+ Example 568
7-Fluoro-3-[ (E) -2- (4-f luorophenyl)-vinyl]-5- (4H-[l,2,4]triazol-3-yl)-1H-indazole MS (ESI) m/z 324 MH+ Example 569
6-Fluoro-3-{(E)-2-(4-fluorophenyl)-vinyl}-5-(5-methyl-4H-[1,2,4]triazol-3-yl)-lH-indazole MS (ESI) m/z 338 MH+ Example 570
(5-{6-Fluoro-3-[(E)-2~(4-fluorophenyl)-vinyl]-1H~ indazol-5-yl}-4H- [1,2,4]triazol-3-yl)-methanol MS (ESI) m/z 354 MH+ Example 571
(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H- [1,2,4]triazol-3-ylmethyl)-methyl-amine MS (ESI) m/z 367 MH+ Example 572
(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H-[1,2, 4]triazol-3-ylmethyl)-dimethyl-amine MS (ESI) m/z 381 MH+ Example 573
C-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1,2,4]triazol-3-yl)-methylamine
MS (ESI) m/z 353 MH+ Example 574
(15)-1-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-AH-[1,2,4]triazol-3-yl)-2-methyl-propylamine MS (ESI) m/z 395 MH+ Example 575
(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H- [1,2,4]triazol-3-ylmethyl)-dimethyl-amine MS (ESI) m/z 381 MH+ Example 57 6
C-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl] -1H-indazol-5-yl}-4H- [1,2,4]triazol-3-yl)-methylamine MS (ESI) m/z 353 MH+ Example 577
(1S)-1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[l,2,4]triazol-3-yl)-2-methyl-propylamine MS (ESI) m/z 395 MH+ Example 578
2- (5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[l,2,4]triazol-3-yl)-propan-2-ol MS (ESI) m/z 382 MH+ Example 579
2-(5-{6-Fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1,2, 4]triazol-3-yl)-propan-2-ol MS (ESI) m/z 382 MH+ Example 580
1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-4H-[l,2,4]triazol-3-yl)-1-methyl-ethylamine
MS (ESI) m/z 382 MH+
Example 581
1-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole
-5-yl}-4H- [1,2,4]triazole-3-yl)-1-methyl- ethylamine
MS (ESI) m/z 382 MH+
Example 582
[(1S)-1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-4H-[1,2, 4]triazol-3-yl)-2-methyl-propyl]-
methyl-amine
MS (ESI) m/z 409 MH+
Example 583
1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-
5-yl}-4H-[l,2,4]triazol-3-yl)-cyclopropylamine
MS (ESI) m/z 379 MH+
Example 584
1-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-
5-yl}-4H-[1, 2, 4]triazol-3-yl)-cyclopropylamine
MS (ESI) m/z 379 MH+
Example 585
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-[5-(thiophen-2-
yl)methyl-4H-[l,2,4]triazol-3-yl]-lH-indazole
MS (ESI) m/z 379 MH+
Example 58 6
5-(5-Benzyl-4H-[l,2,4]triazol-3-yl)-6-fluoro-3-[(E)-2-
(4-fluorophenyl)-vinyl]-lH-indazole MS (ESI) m/z 379 MH+ Example 587
(1S) -1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-AH- [1,2,A]triazol-3-yl)-2-phenyl-ethylamine MS (ESI) m/z 443 MH+ Example 58 8
[2-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H~ indazol-5-yl}-4H- [1,2,4]triazol-3-yl)-ethyl]-dimethyl-ethylamine
MS (ESI) m/z 395 MH+ Example 58 9
[2-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1, 2, 4]triazol-3-yl)-ethyl]-dimethyl-ethylamine MS (ESI) m/z 395 MH+ Example 590
5- [5- (Azetidin-3-yl) -AH- [1,2, 4 ] triazol-3-yl] -6-f luoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole MS (ESI) m/z 379 MH+ Example 591
5-[5- (Azetidine-3-yl) -AH- [1, 2, 4] triazol-3-yl]-7-f luoro-3-[ ( E) -2- (4-fluorophenyl)-vinyl]-1H-indazole MS (ESI) m/z 379 MH+ Example 592 6-Fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-5-(1-methyl-lH-
[1,2,4]triazol-3-yl)-lH-indazole MS (ESI) m/z 338 MH+ Example 593
5-(1, 5-Dimethyl-1H-[1, 2, 4]triazol-3-yl) -6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole MS (ESI) m/z 352 MH+ Example 594
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(1-methyl-1H-[1,2, 4]triazol-3-yl) -1H-indazole MS (ESI) m/z 338 MH+ Example 595
2-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H- [1,2,4]triazol-3-yl)-acetamide
After reacting 15 mg of 6-fluoro-3-{(E)-2-(4-f luorophenyl) vinyl}-liJ-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 559 and 18 mg of hydrazinocarbonylacetic acid ethyl ester in accordance with Example 371, the reaction solution was stirred at 70°C for 10 hours in 5 mL of 2N ammonia-ethanol solution. After distilling off the solvent, purification by LC-MS was conducted, to give 4.62 mg of the title compound. MS (ESI) m/z 381 MH+ Example 596
2-(5-{7-Fluoro-3-[(E)-2~ (4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H-[l,2,4]triazol-3-yl)-acetamide
From 15 mg of 7-fluoro-3-[[E)-2-(4-
fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl
ester hydrochloride obtained by Example 383, 1. 42 mg of the title
compound was obtained in accordance with Example 595.
MS (ESI) m/z 381 MH+
Example 597
[l-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}—[1,3,4]oxadiazol-2-yl) -1-
methyl-ethyl]-methyl-amine
15 mg of 6-fluoro-3-{(E)-2-(4-fluorophenyl)-vinyl}-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride produced by Example 559 and 19 mg of (1-hydrazinocarbonyl-l-methyl-ethyl)-methyl-carbamic acid tert-butyl ester were dissolved in 1 mL of pyridine, and stirred at 110°C for 12 hours. After allowing to cool to room temperature, water was added and extracted twice with ethyl acetate, and then the solvent was distilled off. The resultant residue was added with 1 mL of 4N hydrochloric acid-ethyl acetate solution, stirred at room temperature for 4 hours, then the solvent distilled off, followed by purification by LC-MS, to give 0.36 mg of the title compound. MS (ESI) m/z 396 MH+ Example 598
(S)-1-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-[1,3,4]oxadiazol-2-yl)-2-methyl-propylamine
15 mg of 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 and 19 mg of
((1S)-l-hydrazinocarbonyl-2-methyl-propyl)-carbamic acid tert-butyl ester were dissolved in 1 mL of 1,4-dioxane, and stirred at 85°C for 12 hours. After distilling off the solvent, 1 mL of 4N hydrochloric acid-ethyl acetate solution was added and stirred at room temperature for 3 hours. After distilling off the solvent, purification by LC-MS was conducted, to give 6.05 mg of the title compound. MS (ESI) m/z 396 MH+ Example 599
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-car boxamidine hydrochloride
400 mg of 6-fluoro-3-{(E)-2-(4-fluorophenyl)-vinyl}-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride produced by Example 559 was dissolved in 20 mL of 2N ammonia-ethanol solution, and stirred at 50°C for 6hours . After allowing to cool to room temperature, was added saturated brine, extracted with a mixed solvent of ethyl acetate : tetrahydrof uran =1:1, dried over magnesium sulfate, and evaporated. After adding 4Nhydrochloricacid-ethyl acetate solutionto the residue, the solvent was evaporated, and the resultant solid was washed with diethyl ether, followed by filtration, to afford 351 mg of the title compound as pale yellow crystals. MS (ESI) m/z 299 MH+ Example 600
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(pyrimidin-2-yl)-lH-indazole
36 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxamidine hydrochloride obtained by Example 599 and 15 µl of 3-dimethylaminopropenal were dissolved in 1 mL of pyridine, and stirred at 120°C for 7 hours. After distilling off the solvent, purification by LC-MS was conducted, to give 0 . 61 mg of the title compound.
MS (ESI)m/z 335 MH+ Example 601
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(4-methyl-pyrimidin-2-yl)-lH-indazole
From 20 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxamidine hydrochloride obtained by Example 599 and 14 mg of 4-dimethylamino-3-buthene-2-on, 1.94 mg of the title compound was obtained in accordance with Example 600.
1H-NMR (400 MHz, CD3OD) δ 2.66 (3H, s), 7.12 (2H, t, J = 8.8 Hz), 7.36 (1H, d, J = 10.8 Hz) , 7.39 (1H, d, J=4.8Hz), 7.42 (1H, d, J = 16.4 Hz), 7.57 (1H, d, J = 16.4 Hz), 7.67 (1H, dd, J = 8.8, 5.2 Hz), 8.62 (1H, d, J = 6.8 Hz), 8.75 (1H, d, J = 4.8 Hz) Example 602
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(5-methyl-pyrimidin-2-yl)-lH-indazole
From 20 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxamidine
hydrochloride obtained by Example 599 and 14 mg of (E)-3-dimethylamino-2-buthenal, 8.43 mg of the title compound was obtained in accordance with Example 600.
1H-NMR (400 MHz, CD3OD) δ 2.43 (3H, s) , 7.12 (2H, t, J = 8.8 Hz), 7.35 (1H, d, J = 11.2 Hz), 7.40 (1H, d, J = 16.8 Hz), 7.56 (1H, d, J = 16.8 Hz) , 7. 67 (1H, dd, J = 8.8, 5.2 Hz) , 8.64 (1H, d, J = 6.8 Hz), 8.78 (2H, s) Example 603
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(lH-imidazol-2-yl)-lH-indazole
After suspending 108 mg of 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-car boxymidic acid ethyl ester hydrochloride obtained by Example 383 in 2 mL of ethanol, 206 µl of triethylamine and 50 µl of aminoacetoaldehyde ethylacetal were added, and stirred at 70°C for 5 hours . After cooling to room temperature, water was added, extracted with ethyl acetate, and the resultant layer was washed with saturated brine and dried over anhydrous sodium sulfate. After evaporating the solvent, the resultant crude product was dissolved in 5 mL of tetrahydrofuran, added with 5 mL of 5N hydrochloric acid and stirred at room temperature overnight. After neutralization by adding saturated sodium hydrogen carbonate, the react ion mixture was extracted with ethyl acetate, and washed with water and saturated brine. The organic layer was dried over magnesium sulfate, and the solvent was evaporated. The resultant crude product was purified by preparative TLC,
to obtain 36.5 mg of the title compound as yellow crystals.
MS (ESI) m/z 323 MH+
Production example 604
5-(l-Dimethylsulfamoyl-lH-imidazol-2-yl)-7-fluoro-3-
[(E)-2-(4-fluorophenyl)-vinyl]-indazole-1-sulfonic acid
dimethylamide
36.5 mg of 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(lH-imidazol-2-yl) -lH-indazole obtained by Example 603 was dissolved in N,N-dimethylformamide, and added with 14 mg of sodium hydride (containing 60%) under ice cooling and stirred for 10 minutes. Adding dropwise with 30.3 µl of N,N-dimethylsulfamoyl chloride, the solution was warmed to room temperature and stirred overnight. After stopping the reaction by adding water, the solution was extracted with ethyl acetate, and the organic layer was washed with water and saturated brine. After drying the organic layer over magnesium sulfate, the solvent was evaporated. The crude product was purified by preparative TLC, to afford 14.2 mg of the title compound. 1H-NMR (400 MHz, CDCl3) δ 2.60 ( 6H, s ), 3.17 ( 6H, s ), 7.09 ( 2H, t, J = 8.4 Hz ), 7.17 ( 1H, d, J = 1.6 Hz ), 7.24 (1H, d, J = 16.8 Hz ), 7.51 ( 1H, d, J = 1.6 Hz ), 7.53-7.59 (2H, m ), 7.60 ( 1H, d, J = 16.8 Hz ), 7.68 ( 1H, dd, J = 11.6, 1.2 Hz ), 8.26 ( 1H, d, J = 1.6 Hz ) Example 605
(2-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-3H-imidazol-4-yl)-methanol
14.2 mg of 5-(1-dimethylsulfamoyl-1H-imidazol-2-yl)-7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-indazole-1-sulof onic acid dimethylamide obtained by Production example 604 was dissolved in 1 mL of tetrahydrofuran, added at -78 °C with 20.1 ul of 1.58 M n-butyllithium in hexane, stirred at this temperature for 10 minutes, then added with 5 pi of benzylchloromethylether, warmed to room temperature, and stirred for 20 minutes. After stopping the reaction by adding water, the solution extracted with ethyl acetate, and the organic layer was washed with water and saturated brine and then dried over anhydrous magnesium sulfate. After evaporating the solvent, the resultant crude product was dissolved in 2 mL of aqueous hydrogen bromide, and stirred at 60°C overnight. After cooling to room temperature, the reaction solution was neutralized with saturated aqueous sodium hydrogen carbonate and extracted with ethyl acetate. The organic layer was washed with water and saturated brine, and then dried over anhydrous magnesiumsulfate. After evaporating the solvent,purification by LC-MS was conducted, to obtain 0.58 mg of the title compound. MS (ESI) m/z 353 MH+ Example 606
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(lH-imidazol-2-yl)-lH-indazole
By treating 108 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtainedby Example 559 in the similarmethod

as described in Example 603, 34.4 mg of the title compound was obtained as white crystals. MS (ESI) m/z 323 MH+ Production example 607
5-(l~Dimethylsulfamoyl-1H-imidazol-2-yl)-6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-indazole-1-sulfonic acid dimethylamide
34.4 mg of 6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-5-(lH-imidazol-2-yl) -1H-indazole
obtained by Example 606 was treated in the similar method as
described in Production example 604, to afford 29.5 mg of the
title compound.
1H-NMR ( 400MHz, CDCl3 ) δ 2.71 ( 6H, s ) , 3. 03 ( 6H, s ) , 7.06-7.12
( 2H, m ) , 7.24 ( 1H, d, J = 2.0 Hz ) , 7.50 (1H, d, J = 1.2 Hz ) ,
7.53-7.63 ( 3H, m ), 7.69 ( 1H, d, J = 16.4 Hz )
Example 608
(2-{ 6-Fluoro-3- [ (E) -2- (4-fluorophenyl) -vinyl]-lH-indazol-5-
yl}-3H-imidazol-4-yl)-methanol
29.5 mg of 5-(1-dimethylsulfamoyl-lH-
imidazol-2-yl)-6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-
indazole-1-sulfonic acid dimethylamide obtained by Production
example 607 was treated in the similar method as described in
Example 605, to afford 0.54 mg of the title compound.
MS (ESI) m/z 353 MH+
Example 609
5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-4H-[l,2,4]triazol-3-ol
20 mg of 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 was dissolved in 3 mL of pyridine, added with 6.1 mg of semicarbazide hydrochloride and refluxed for 8 hours. After cooling to room temperature, the solvent was distilled off under reduced pressure, followed by purification by LC-MS, to afford 1.66 mg of the title compound. 1H-NMR (4 00 MHz, CD3OD ) δ7.15 (2H, t, J=8.8Hz), 7.41 (1H, d, J = 16.8 Hz ), 7.62 ( 1H, d, J = 16.8 Hz ), 7.66 ( 1H, d, J = 12.4 Hz ), 7.70 ( 1H, dd, J = 6.0, 8.8 Hz ), 8.32 (1H, s ) MS (ESI) m/z 340 MH+ Example 610
5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-4H-[l,2,4]triazol-3-ylamine
20 mg of 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 was dissolved in 3 mL of pyridine, added with 6.1 mg of aminoguanidine hydrochloride, and refluxed for 8 hours. After cooling to room temperature, the solvent was evaporated, followed by purification by LC-MS, to afford 0.72 mg of the title compound. 1H-NMR ( 400 MHz, CD3OD ) δ 7.15 ( 2H, t, J = 8 . 8 Hz ) , 7.43 ( 1H, d, J = 16.8 Hz ), 7.61 ( 1H, d, J = 16.4 Hz ), 7.67-7.73 ( 3H, m ), 8.44 (1H, d, J = 1.2 Hz ) MS (ESI) m/z 339 MH+
Example 611
4-Amino-5-{7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-2,4-dihydro-[1,2,4]triazol-3-one
20 mg of 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 was dissolved in 5 mL of ethanol, added with 5. 5 mg of carbohydrazide and ref luxed overnight. The solvent was evaporated, followed by purification by LC-MS, to afford 1.47 mg of the title compound. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 5.49 ( 2H, s ), 7.24 ( 2H, t, J = 8.8 Hz ), 7.53 ( 2H, s ), 7.72-7.80 ( 3H, m ), 8.68 (1H, s ), 11.9 (1H, s ) MS (ESI) m/z 355 MH+ Example 612
5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[1,2,4]triazol-3-ylamine
20 mg 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 559 was treated in accordance with Example 610, to afford 0.64 mg of the title compound.
1H-NMR ( 400 MHz, CD3OD ) δ 7.14 ( 2H, t, J = 8.8 Hz ) , 7.41 ( 1H, d, J = 17.2 Hz ), 7.44 ( 1H, d, J = 10.8 Hz ), 7.58 ( 1H, d, J = 16.8 ), 7.69 ( 1H, dd, J = 5.4, 8.4 Hz ), 8.62 (1H, d, J = 6.4 Hz ) MS (ESI) m/z 339 MH+
Example 613
5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-4H-[1, 2,4]triazol-3-ol
20 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 559 was treated in accordance with Example 609, followed by purification by preparative TLC, to afford 1.55 mg of the title compound as white crystals.
1H-NMR (4 00 MHz, DMSO-D6 ) δ7.24 (2H, t, J=8.8Hz), 7.47-7.54 ( 3H, m ), 7.76-7.80 ( 2H, m ), 8.48 (1H, d, J = 6.4 Hz ) MS (ESI) m/z 340 MH+ Example 614
4-Amino-5-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-2,4-dihydro-[1,2,4]triazol-3-one
20 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 559 was treated in the similar method as described in Example 611, f ollowedby purif icationby preparative TLC, to afford 2.19 mg of the title compound as white crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 5.27 ( 2H, s ), 7.24 ( 2H, t, J = 8.7 Hz ) , 7.4 7 ( 1H, d, J = 10.8 Hz ) , 7.52 ( 2H, s ) , 7.77-7.80 (2H, m ), 8.43 ( 1H, d, J = 6.0 Hz ) MS (ESI) m/z 355 MH+ Example 615 5-(lH-Benzimidazol-2-yl)-6-fluoro-3-[ (E) -2- (4-
fluorophenyl) vinyl] -1H-indazole
To a solution of 10 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxydimic acid ethyl ester hydrochloride obtained by Example 559 in 0.5 mL of acetic acid was added 85 µl of phenylene diamine (in 1 molar acetic acid) at room temperature and stirred at 120°C for a day. Following addition of water and extraction with ethyl acetate, the organic layer was concentrated. The residue was separated and purified by LC-MS, to afford 10.08 mg of the title compound as colorless crystals. MS (ESI)m/z 373 MH+ Example 616
The compounds of Examples 617-623 were synthesized from 6-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-lH-indazole-5-carboxydimic acid ethyl ester hydrochloride and commercially available substituted phenylene diamine in accordance with the method of Example 615. Example 617
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(7-methyl-lH-benzimidazol-2-yl) -1H-indazole MS (ESI)m/z 387 MH+ Example 618
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(6-methyl-lH-benzimidazol-2-yl) -1H-indazole MS (ESI)m/z 387 MH+ Example 619
5-(6-Chloro-1H-benzimidazol-2-yl)-6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole MS (ESI)m/z 407 M+ Example 620
6-Fluoro-5-(6-fluoro-1H-benzimidazol-2-yl)-3-[(E)-2- (4-fluorophenyl)-vinyl]-1H-indazole MS (ESI)m/z 391 MH+ Example 621
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-(6-nitro-1H-benzimidazol-2-yl)-lH-indazole MS (ESI)m/z 418 MH+ Example 622
5-(5,6-Dichloro-1H-benzimidazol-2-yl)-6-fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-lH-indazole MS (ESI)m/z 441 M+ Example 623
5-(5-Chloro-6-fluoro-lH-benzimidazol-2-yl)-6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole MS (ESI)m/z 425 M+ Production example 624 5-Bromo-lH-indazole-3-carboxylic acid
A mixture of 15 g of 5-bromoisatine, 34 mL of water and 2.79 g of sodium hydroxide was stirred at 50°C for 5 minutes, and then under stirring on ice, added with a solution containing 4.58 g of sodium sulfite in 16 mL of water. The reaction mixture was added dropwise under cooling on ice to a mixed solution of
6.72 mL concentrated sulfuric acid/ 133 mL water in such a speed that did not cause the temperature of the reaction solution to exceed 4°C. After stirring for 15 minutes under ice cooling, a solution containing 30.2 g of tin chloride (II) in 56 mL of concentrated hydrochloric acid was added dropwise in such a speed that did not cause the temperature of the reaction solution to exceed4°C, followedby 1-hour stirring on ice . Then the reaction mixture was added with water and extracted with a mixed solvent of ethyl acetate :tetrahydrofuran=l: 1, and the resultant organic layer was washed with saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the generated crystals were washed with diethyl ether and filtered, to obtain 4.64 g of the title compound as pale yellow crystals. 1H-NMR (4 00 MHz, DMSO-D6) δ 7 . 54 (1H, dd, J = 8.8, 2.0 Hz) , 7.63 (1H, d, J = 8.8 Hz), 8.19 (1H, d, J = 2.0 Hz) Production example 625 5-Bromo-lH-indazole-3-carboxylic acid ethyl ester
3 g of 5-bromo-lH-indazole-3-carboxylic acid was dissolved in 60 mL of ethanol, added with 0. 6 mL of concentrated sulfuric acid, and heated under reflux for 8 hours. After allowing to cool to room temperature, addition of water and extraction with ethyl acetate were conducted, and the resultant organic layer was washed with saturated aqueous sodium hydrogen carbonate and saturated brine, dried over magnesium sulfate, and the solvent was evaporated, to afford 2.7 g of the title compound as yellow crystals.
1H-NMR (400 MHz, CDCl3) δ 1.50 (3H, t, J = 7.2 Hz), 4.54 (2H,
q, J = 7.2 Hz), 7.54 (2H, d, J = 1.2 Hz), 8.37 (1H, t, J = 1.2
Hz)
Production example 626
5-Bromo-l-trityl-lH-indazole-3-carboxylic acid
2.19 g of 5-bromo-lH-indazole-3-carboxylic acid ethyl ester was dissolved in 40 mL of dimethylformamide, added with 391 mg of 60% sodium hydride(oil-based) and 2.38 g of trityl chloride, and stirred at room temperature for 1 hour. After adding a saturated aqueous ammonium chloride, the reaction solution was extracted with ethyl acetate, and the resultant organic layer was washed with saturated brine, dried over magnesium sulfate, and the solvent was evaporated. The resultant residue was dissolved in a solution of 20 mL of ethanol, 20 mL of tetrahydrofuran and 15 mL of 5N sodium hydroxide aqueous, and stirred at 60°C for 2 hours. After allowing to cool to room temperature, the reaction solution was neutralized by adding 1N hydrochloric acid, extracted with a mixed solvent of ethyl acetate :tetrahydrofuran=l: 1, and the resultant organic layer was washed with saturated brine and dried over magnesium sulfate. After evaporated the solvent, the generated crystals were washed with diethyl ether and filtered to afford 3.3 g of the title compound as pale yellow crystals. MS (ESI) m/z 481, 483 (M-H)~ Production example 627 5-Bromo-l-trityl-lH-indazole-3-carboxylic acid phenylamide
Fromlg of 5-bromo-l-trityl-1H-indazole-3-carboxylic acid and 0.23 mL of aniline, 755 mg of the title compound was obtained as pale yellow crystal in accordance with Example 127. 1H-NMR (400 MHz, CDCl3) δ 6.28 (1H, d, J = 8.8 Hz), 6.65-6.70 (1H, m) , 6.7 5 (1H, t, J = 6.8 Hz) , 7.06-7.39 (17H, m) , 7.56 (2H, d, J = 8.8 Hz), 8.55 (1H, brs), 8.62 (Ih, d, J = 2.0 Hz) Production example 628 5-Amino-l-trityl-lH-indazole-3-carboxylic acid phenylamide
From 318 mg of 5-bromo-l-trityl-lH-indazole-3-carboxylic acid phenylamide, 258 mg of the title compound was obtained in accordance with Production example 422.
1H-NMR (400 MHz, CDCl3) δ 6.20 (IH, d, J = 8.8 Hz), 6.47 (IH, dd, J = 8.8, 2.4 Hz), 7.02-7.82 (21H, m), 8.59 (IH, brs) Production example 629 3-Phenylcarbamoyl-l-trityl-lH-indazole-5-carboxylic acid
470 mg of 5-bromo-l-trityl-lH-indazole-3-carboxylic acid phenylamide obtained by Production example 627 was dissolved in 8 . 4 mL of tetrahydrof uran, and added with 1.58 mL of 1.6 M n-butyllithium hexane solution under stirring at -70°. After stirring at -70 °C for 15 minutes, dry ice-ethanol bath was removed, and carbon dioxide was blown into the reaction for 25 minutes. After adding a saturated aqueous ammonium chloride, the reaction solution was extracted with ethyl acetate, and the resultant organic layer was washed with saturated brine and dried over magnesium sulfate. The solvent was evaporated, and the generated crystals were washed with a mixed solvent of
hexane: diethyl ether =2:1 and filtered, to afford 230 mg of the
title compound as pale yellow crystals.
1H-NMR (400 MHz, CDCl3) δ 6.46 (1H, d, J = 9.2 Hz), 6.98-7.45
(18H, m) , 7.54-7.64 (2H, m), 7.73 (1H, dd, J = 8.8, 1.6 Hz),
8.58 (1H, s), 9.25 (1H, s)
Example 630
5-Amino-l-trityl-1H-indazole-3-carboxylic acid phenylamide produced by Production example 628 and various kinds of carboxylic acid were treated in the similar method as described in Example 183, to afford the compounds of Examples 631-635. Example 631
5-Acetylamino-lH-indazole-3-carboxylic acid phenylamide MS (ESI) m/z 295 MH+ Example 632
5-Cyclopropane carbonylamino-lH-indazole-3-carboxylic acid phenylamide MS (ESI) m/z 321 MH+ Example 633
5-[((2S)-5-Oxopyrrolidine-2-carbonyl)-amino]-lH-indazole-3-carboxylic acid phenylamide MS (ESI) m/z 364 MH+ Example 634
5-[(Furan-2-carbonyl)-amino]-1H-indazole-3-carboxylic acid phenylamide MS (ESI) m/z 347 MH+ Example 635
5-[2-(Thiophen-2-yl)-acetylamino]-lH-indazole-3-carboxylic
acid phenylamide
MS (ESI) m/z 377 MH+
Example 636
5-Methanesulfonylamino-lH-indazole-3-carboxylic acid
phenylamide
10 mg of 5-amino-l-trityl-lH-indazole-3-carboxylic acid phenylamide obtained by Production example 628 was dissolved in dichloromethane, added with 6 µl triethylamine and 4 mg of methanesulfonyl chloride, and stirred at room temperature for 3 hours. Following addition of water and extraction with ethyl acetate, the residue obtained by evaporating the solvent was added with 0 . 5mL of dichloromethane and 0 . 5mL of trifluoroacetic acid, and stirred at room temperature for 1 hour. After evaporating the solvent, purification by LC-MS was conducted, to afford 0.22 mg of the title compound. MS (ESI) m/z 331 MH+ Example 637
3-Phenylcarbamoyl-l-trityl-1H-indazole-5-carboxylic acid produced by Production example 629 and various kinds of amine were amidated in the similar method as described in Example 44 , followed by deprotection in accordance with the deprotection condition of Example 183, and purification by LC-MS, to afford the compounds of Examples 638-64 0. Example 638 3-Phenylcarbamoyl-lH-indazole-5-carboxylie acid
[(1S)-l-hydroxymethyl-2-methylpropyl]-amide MS (ESI)m/z 367 MH+ Example 639
3-Phenylcarbamoyl-1H-indazole-5-carboxylic acid [(1S)-2-hydroxy-l-phenylethyl]-amide MS (ESI)m/z 401 MH+ Example 640
3-Phenylcarbamoyl-1H-indazole-5-carboxylic acid [(1S)-1-carbamoylethyl]-amide MS (ESI)m/z 352 MH+ Example 641 3-Phenylcarbamoyl-2H-indazole-5-carboxylic acid
10 mg of 3-phenylcarbamoyl-l-trityl-2H-indazole-5-carboxylic acid produced by Production example 629 was dissolved in 0.5 mL of dichloromethane and 0. 5 mL of trif luoroacetic acid, and stirred at room temperature for 3 hours, followed by purification by LC-MS, to afford 0.72 mg of the title compound. MS (ESI)m/z 282 MH+ Production example 642
6-Fluoro-3-[(E)-2-(2-fluorophenyl)-vinyl]-5-nitro-l-trityl-lH-indazole
From 2.0 g 3-bromo-6-fluoro-5-nitro-l-trityl-lH-indazole obtainedby Production example 180 and 1. 46 g of 2-f luorostyrene, 1.15 gof the title compound was obtained as bright yellow crystals in accordance with the method of Example 181.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 6.13 ( 1H, d, J = 12.8 Hz ), 7.17
- 7.45 ( 18H, m ), 7.54 ( 1H, d, J = 16.8 Hz ), 7.81 ( 1H, d,
J = 16.8 Hz ), 8.01 ( 1H, t, J = 7.6 Hz ), 9.15 ( 1H, d, J =
7.2 Hz )
Production example 64 3
6-Fluoro-3-[(E)-2-(2-fluorophenyl)-vinyl]-1-trityl-1H-
indazol-5-ylamine
From 1.14 g of 6-fluoro-3-[ (E)-2-(2-fluorophenyl)-vinyl]-5-nitro-l-trityl-lH-indazole obtained by Production example 642, 1.07 g of the title compound was obtained as bright yellow crystals in accordance with the method of Example 182. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 5.09 ( 2H, br s ) , 5.92 ( 1H, d, J = 12.4 Hz ), 7.16 - 7.38 ( 20H, m ), 7.41 ( 1H, d, J = 16.8 Hz ) , 7.87 ( 1H, t, J = 7.6 Hz ) Production example 644
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-5-nitro-l-trityl-lH-indazole
In accordance with the method of Example 181, from 1.5 g of 3-bromo-6-fluoro-5-nitro-l-trityl-lH-indazole obtained by Production example 180 and 730 mg of 3-fluorostyrene, 872 mg of the title compound was obtained as bright yellow crystals. 1H-NMR (4 00 MHz, DMSO-D6 ) δ 6.12 ( 1H, d, J = 12.8 Hz ) , 7.15 ( 1H, dt, J = 2.0, 8.8 Hz ), 7.20-7.45 ( 16H, m), 7.48 ( 1H, d, J = 16.4 Hz ), 7.54 (1H, d, J = 8.0 Hz), 7.69 ( 1H, d, J = 10.8 Hz ), 7.77 ( 1H, d, J = 16.4 Hz ), 9.19 ( 1H, d, J = 7.2 Hz )
Production example 645
6-Fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1-trityl-lH-
indazol-5-ylamine
In accordance with the method of Example 182, from 870 mg of 6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-5-nitro-l-trityl-2H-indazole obtained by Production example 644, 707 mg of the title compound was obtained as bright yellow crystals. The instrumental data coincided with that of Production example 174.
Production example 64 6
6-Fluoro-5-nitro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-trityl-lH-indazole
In accordance with the method of Example 181, from 1.0 g of 3-bromo-6-fluoro-5-nitro-l-trityl-lH-indazole obtained by Production example 180 and 314 mg of 3-vinylpyridine, 588 mg of the title compound was obtained as bright yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 6.14 ( 1H, d, J = 12.8 Hz ), 7.10 - 7.45 ( 16H, m ), 7.50 ( 1H, d, J = 16.8 Hz ), 7.81 ( 1H, d, J = 16.8 Hz ), 8.01 ( 1H, d, J = 8.0 Hz ), 8.50 ( 1H, dd, J = 2.0, 4.8 Hz ), 8.90 ( 1H, d, J = 2.0 Hz ), 9.19 ( 1H, d, J = 7.2 Hz )
Production example 647
6-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-l-trityl-1H-indazol-5-ylamine
In accordance with the method of Example 182, from 587 mg of 6-fluoro-5-nitro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1-
trityl-lH-indazole obtained by Production example 646, 465 mg of the title compound was obtained as bright yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 4. 99 ( 2H, br s ) , 5. 90 ( 1H, d, J = 12.4 Hz ) , 7.13-7.45 ( 18H, m ) , 7.46 ( 1H, d, J = 16.8 Hz ), 8.11 ( 1H, d, J = 7.6 Hz ), 8.43 ( 1H, d, J = 2.0, 4.4 Hz ) , 8.75 ( 1H, d, J = 2.0 Hz) Production example 648
6-Fluoro-5-nitro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1-trityl-1H-indazole
In accordance with the method of Example 181, from 2.0 g of 3-bromo-6-fluoro-5-nitro-l-trityl-lH-indazole obtained by Production example 180 and 1.10 g of 2-vinylthiophene, 684 mg of the title compound was obtained as yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 6.14 ( 1H, d, J= 12.4 Hz ), 7.11 ( 1H, dd, J = 3,6, 4.8 Hz ), 7.17 - 7.45 ( 17H, m ), 7.57 ( 1H, d, J = 4.8 Hz ), 7.67 ( 1H, d, J = 16.4 Hz ), 9.15 ( 1H, d, J = 7.2 Hz )
Production example 64 9
6-Fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1-trityl-lH-indazol-5-ylamine
In accordance with the method of Example 182, from 647 mg of 6-fluoro-5-nitro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1-trityl-lH-indazole obtained by Production example 648, 623 mg of the title compound was obtained as bright yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 5.00 ( 2H, br s ), 5.90 ( 1H, d, J = 12.0 Hz ), 7.01 ( 1H, d, J = 16.4 Hz ),7.08 ( 1H, dd, J =
3.6, 5.2 Hz ), 7.16 - 7.44 ( 18H, m ), 7.49 ( 1H, d, J=5.2 Hz ) Production example 650
1-(tert-Butoxycarbonyl-methyl-amino)-cyclopropane carboxylic acid ethyl ester
To a solution of 500 mg of 1-tert-butoxycarbonylamino-cyclopropane carboxylic acid ethyl ester which was obtained from 1,1-cyclopropane dicarboxylic acid diethyl ester in accordance with the method described in the document (Journal of Medicinal Chemistry, 31, 2004 (1988)) in 5 mL of dimethylformamide, 0.2 mL of methyl iodide and 88 mg of 60% sodium hydride were added and stirred at room temperature for 1 hour. After adding water, the reaction solution was extracted with diethyl ether. The organic layer was washed with water and saturated brine, and dried over anhydrous magnesium sulfate. The residue obtained by evaporating the solvent was purified by silica gel column chromatography(ethyl acetate :n-hexane=l:19), to afford 59 mg of the title compound as a pale yellow oil.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.17 ( 3H, t, J = 7.2 Hz ) , 1.22 ( 2H, br s ), 1.36, 1.40 ( 11H, each s ), 2.76, 2.81 ( 3H, each s ), 4.09 ( 2H, q, J = 7.2 Hz ) Production example 651
1-(tert-Butoxycarbonyl-methyl-amino)-cyclopropane carboxylic acid
To a solution of 48 mg of 1-(tert-butoxycarbonyl-methyl-amino)-cyclopropane carboxylic acid ethyl ester in 0.4 mL of methanol, 0.1 mL of 5N sodium hydroxide aqueous solution
was added and stirred at room temperature overnight. After adding water, the reaction solution was washed with diethyl ether. The aqueous layer was added with 0. 7 mL of 1N hydrochloric acid, and extracted with ethyl acetate. The organic layer was washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 36 mg of the title compound as white crystals.
1H-NMR ( 400 MHz, DMS0-D6 ) δ 1.18 - 1.46 ( 4H, m ) , 1.36, 1.39 ( 9H, each s ), 2.76, 2.80 ( 3H, each s ), 12.50 ( 1H, br s ) Production example 652
1-(tert-Butyl-dimethyl-silanyloxymethyl)-cyclopropane carboxylic acid ethyl ester
To a solution of 700 mg of 1-hydroxymethyl-cyclopropane carboxylic acid ethyl ester obtained from 1,1-cyclopropane dicarboxylic acid diethyl ester in accordance with the method described in the document (Tetrahedron Letters, 40, 5467 (1988)) in 7 mL of N,N-dimethylformamide, 430 mg of imidazole and 916 mg of tert-butyldimethylchlorosilane were added and stirred at room temperature overnight. After adding n-hexane to the reaction solution, the organic layer was washed successively withwater, 1Nhydrochloricacid,water, saturated aqueous sodium hydrogen carbonate and saturated brine. The organic layer was dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 1.13 g of the title compound as a colorless oil. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.03 ( 6H, s ), 0.85 ( 11H, S),
1.13 ( 2H, dd, J = 4.0, 6.8 Hz ), 1.17 ( 3H, t, J = 7.2 Hz )
3.74 ( 2H, s ), 4.05 ( 2H, q, J = 7.2 Hz )
Production example 653
1-(tert-Butyl-dimethyl-silanyloxymethyl)-cyclopropane
carboxylic acid
1.10 g of 1-(tert-butyl-dimethyl-silanyloxymethyl)-cyclopropane carboxylic acid ethyl ester was hydrolyzed in the manner as described in Production method 651, to afford 781 mg of the title compound as a colorless oil.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.03 ( 6H, s ) , 0. 81 ( 2H, dd, J = 3.6, 6.8 Hz ) , 0.85 ( 9H, S) , 0.99 ( 2H, dd, J = 3.6, 6.8 Hz ) 3.74 ( 2H, s ), 12.18 ( 1H, br s ) Production example 654
(2S,4R)-4-(tert-Butyl-dimethyl-silanyloxy)-pyrrolidine-2-carboxylic acid methyl ester
To a solution of 2.5 g of 4-(tert-butyl-dimethyl-silanyloxy)-pyrrolidine-1,2-dicarboxylic acid 1-benzylester 2-methyl ester obtained from trans-4-hydroxy-L-proline in accordance with the method of JP-A 62-155279 in 25 mL of methanol was added 250 mg of 20% palladium hydroxide, and stirred overnight at normal pressure under nitrogen atmosphere. After filtering out the catalyst, the solvent was evaporated, to afford 1.64 g of the title compound as a colorless oil.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.78-1.93 ( 2H, m ) , 2.60 ( 1H, dd, J = 2.4, 11.2 Hz ) , 3.03 ( 1H, dd, J = 5.2, 11.2 Hz ) , 3.61 ( 3H, s ), 3.79 ( 1H, t, J = 8.0 Hz ), 4.32 -4.39 ( 1H, m )
Production example 655
(2S,4R)-A-(tert-butyl-dimethyl-silanyloxy)-1-methyl-pyrrolidine-2-carboxylic acid methyl ester
To a solution of 800 mg of (2S,AR)-A-(tert-butyl-dimethyl-silanyloxy)-pyrrolidine-2-carboxylic acid methyl ester in 8 mL of methanol, 0.35 mL of 37% formalin and 80 mg of 10% palladium on carbon were added, and stirred at A atm. Hydrogen pressure for 10 hours. After filtering out the catalyst, the solvent was evaporated, and the residue was separated and purified by silica gel column chromatography (ethyl acetate : n-hexane=l: 6) , to afford 489 mg of the title compound as a colorless oil. 1H-NMR (4 00 MHz, DMSO-D6 ) δ 1.80-1.90 ( 1H, ddd, 4.0, 8.0, 12.0 Hz ) , 2.08 ( 1H, td, J = 4.0, 12.0 Hz ) , 2.20 ( 1H, dd, J = 5.6, 9.2 Hz ), 2.27 ( 3H, s ), 3.15 -3.24 ( 2H, m ), 3.62 ( 3H, s ), 4.32 -4.39 ( 1H, m ) Production example 656
(2S, 4R) -A- (tert-Butyl-dimethyl-silanyloxy) -1-methyl-pyrrolidine-2-carboxylic acid
From 4 88 mg of (2S,4R)-A-(tert-butyl-dimethyl-silanyloxy)-methyl-pyrrolidi ne-2-carboxylic acid methyl ester, 625 mg of the title compound was obtained as white non-crystalline powder in the method as described in Production method 651.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.80-1.90 ( 1H, ddd, 4.0, 8.0,12.0 Hz ) , 2.08 ( 1H, td, J = 4.0, 12.0 Hz ) , 2.20 ( 1H, dd, J = 5.6, 9.2 Hz ), 2.27 ( 3H, s ), 3.15 -3.24 ( 2H, m ), 3.62 ( 3H, s ),
4.32 -4.39 ( 1H, m ) Production example 657
(2S, 4R) -1-Acetyl 4- (tert-butyl-dimethyl-silanyloxy) -pyrrolidine-2-carboxylic acid
To a solution of 800 mg of (2S,4R)-4-(tert-butyl-dimethyl-silanyloxy) -pyrrolidine-2-carboxylic acid methyl ester obtained by Production example 654 in 10 mL of pyridine, 0.35 mL of acetic anhydride was added and stirred at room temperature for 1.5 hours. After adding ethyl acetate to the reaction solution, the organic layer was washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogen carbonate and saturated brine. After drying the organic layer over anhydrous magnesium sulfate, the solvent was evaporated, to obtain crude (25,4R)-l-acetyl-4-(tert-butyl-dimethyl-silanyloxy) -pyrrolidine-2-carboxylic acid methyl ester. Then this ester was hydrolyzed in the manner as described in Production example 651, to afford 337 mg of the title compound as pale red crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.07 ( 6H, s ), 0.85 ( 9H, s ) , 1.92-2.13 ( 2H, m), 1,95 ( 3H, s ), 3.70 ( 1H, dd, J = 5.2, 10.8 Hz ), 4.20 ( 1H, t, J = 8.0 Hz ), 4.46 -4.53 ( 1H, m ), 12.45 ( 1H, br s ) Example 658
Various kinds of amine obtained by Production example 182, Production example 643, Production example 645, Production example 647 and Production example 649, and various kinds of
carboxylic acid obtained by Production example 651, Production example 653, Production example 656 and Production example 657 or commercially available carboxylic acid were amidated in the manner as described in Example 183, followed by deprotection (deprotection of compounds having a tert-butyldimethylsilyl group was conducted using 95% trifluoroacetic acid) and purification by LC-MC, to afford the compounds of Examples 659-688. Example 659
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(2-f luorophenyl) -vinyl] -lH-indazol-5-yl} -amide MS (ESI) m/z 340 MH+ Example 660
N-{6-Fluoro-3-[(E)-2-(2-fluorophenyl)-vinyl]-1H-indazol-5-yl}-acetamide MS (ESI) m/z 314 MH+ Example 661
N-{6-Fluoro-3- [ (E) -2- (2-fluorophenyl) -vinyl] -lH-indazol-5-yl}-3-hydroxy-2,2-dimethyl-propionamide MS (ESI) m/z 372 MH+ Example 662
N-{6-Fluoro-3-[(E)-2-(2-fluorophenyl)-vinyl]-lH-indazol-5-yl}-3-hydroxy-3-methyl-butylamide MS (ESI) m/z 372 MH+ Example 663 N-{6-Fluoro-3-[(E)-2-(2-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-3-hydroxy-propionamide MS (ESI) m/z 344 MH+ Example 664
N-{6-Fluoro-3- [ (E)-2- ( 4-f luorophenyl) -vinyl] -lH-indazol-5-yl}-3-hydroxy-3-methyl-butylamide MS (ESI) m/z 372 MH+ Example 665
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-3-hydroxy-propionamide MS (ESI) m/z 344 MH+ Example 666
N-{ 6-Fluoro-3- [ (E) -2- (4-fluorophenyl) -vinyl] -lH-indazol-5-yl}-3-hydroxy-butylamide MS (ESI) m/z 358 MH+ Example 667
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl} - (3R) -3-hydroxy-3-phenyl-propionamide MS (ESI) m/z 420 MH+ Example 668
Cyclopropane-1,1-dicarboxylic acid amide {6-fluoro-3-[[E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 383 MH+ Example 669
N-{6-Fluoro-3-[(E)-2-(pyridine-3-yl)-vinyl]-lH-indazol-5-yl}-3-hydroxy-2,2-dimethyl-propionamide MS (ESI) m/z 355 MH+
Example 670
N-{6-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-3-hydroxy-3-methyl-butylamide MS (ESI) m/z 355 MH+ Example 671
1-Hydroxy-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 339 MH+ Example 672
Cyclopropane-1,1-dicarboxylic acid amide {6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 366 MH+ Example 673
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 323 MH+ Example 674
N- {6-Fluoro-3-[(E)-2-(pyridin-3-yl)-vinyl]-1H-indazol-5-yl}-acetamide MS (ESI) m/z 297 MH+ Example 675
cis-2-Hydroxy-cyclopentane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 384 MH+ Example 676 1-Hydroxy-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-
(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 356 MH+ Example 677
(2S,4R)-4-Hydroxy-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 385 MH+ Example 67 8
1-Hydroxymethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 320 MH+ Example 67 9
1-Hydroxymethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 370 MH+ Example 680
1-Hydroxymethyl-cyclopropane carboxylic acid {6-fluoro-3-[{E)-2-(thiophen-2-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 358 MH+ Example 681
1-Methylamino-cyclopropane carboxylic acid {6-fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 369 MH+ Example 682
1-Methylamino-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 369 MH+
Example 683
1-Methylamino-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 357 MH+ Example 684
1-Hydroxy-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 356 MH+ Example 685
1-Hydroxy-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 344 MH+ Example 686
2-Cyclopropyl-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl] -2H-indazol-5-yl} -acetamide MS (ESI) m/z 354 MH+ Example 687
(2S,4R)-4-Hydroxy-l-methyl-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl} -amide
MS (ESI) m/z 399 MH+ Example 688
(2S,4R)-l-Acetyl-4-hydroxy-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl} -amide MS (ESI) m/z 427 MH+
Production example 689
1-(6-Fluoro-5-nitro-lH-indazole-l-yl)-ethanone
A mixed solution of 10 g of N- (5-fluoro-2-methyl-4-nitrophenyl)-acetamide obtained by Production example 177, 40 mL of glacial acetic acid and 13.4 mL of acetic anhydride was added dropwise with 10 .1 mL of isoamyl nitrite while heating to 80°C, and after completion of the dropping, the mixture was stirred at 90°C for 3 hours. The solvent was evaporated, and the precipitated crystals were collected by filtration, to afford 1.71 g of the title compound as bright yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 2.76 ( 3H, s ), 8.27 ( 1H, d, J = 11.6 Hz ), 8.68 ( 1H, s ), 8.88 ( 1H, d, J = 7.6 Hz ) Production example 690 6-Fluoro-5-nitro-1H-indazole
1.6 g of 1-(6-fluoro-5-nitro-lH-indazole-l-yl)-ethanone was hydrolyzed in accordance with the method of Production example 409, to afford 1.26 g of the title compound as pale red crystals. The instrumental data coincided with that of Production example 179. Example 691
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine synthesized by Production example 182 and various kinds of carboxylic acid were treated in the similar method as described in Example 183, to afford the compounds of Examples 692-730.
Example 692
2-Amino-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-3-methyl-butylamide
MS (ESI) m/z 371 MH+
Example 693
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-3-methyl-2-methylamino-butylamide
MS (ESI) m/z 385 MH+
Example 694
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-benzamide
MS (ESI) m/z 376 MH+
Example 695
Pyridine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 377 MH+
Example 696
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-nicotinamide
MS (ESI) m/z 377 MH+
Example 697
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-isonicotinamide
MS (ESI) m/z 377 MH+
Example 698
Pyrimidine-5-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 378 MH+ Example 699
Pyrazine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl)-amide MS (ESI) m/z 378 MH+ Example 7 00
Thiophene-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-f luorophenyl) -vinyl] -lH-indazol-5-yl} -amide MS (ESI) m/z 382 MH+ Example 7 01
l-Methyl-lH-pyrrole-2-carboxylic acid {6-fluoro-3-[ (E) -2- (4-fluorophenyl) -vinyl] -2H-indazol-5-yl}-amide MS (ESI) m/z 379 MH+ Example 702
lH-Pyrrole-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 365 MH+ Example 703
5-Methyl-isoxazole-4-carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-yl }-amide
MS (ESI) m/z 381 MH+ Example 704
3,5-Dimethyl-isoxazole-4-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide
MS (ESI) m/z 395 MH+ Example 7 05
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-2,6-dimethoxy-nicotinamide MS (ESI) m/z 437 MH+ Example 7 06
6-Hydroxy-pyridine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 393 MH+ Example 7 07
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-2-methoxy-nicotinamide MS (ESI) m/z 407 MH+ Example 708
N-{ 6-Fluoro-3- [ (E) -2- (4-f luorophenyl) -vinyl] -1H-indazol-5-yl}-2-hydroxy-nicotinamide MS (ESI) m/z 393 MH+ Example 70 9
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-6-hydroxy-nicotinamide MS (ESI) m/z 393 MH+ Example 710
Cyclobutane carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-yl }-amide MS (ESI) m/z 354 MH+
Example 711
1-Cyano-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 365 MH+ Example 712
N-{ 6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-2,2-dimethyl-propioneamide MS (ESI) m/z 356 MH+ Example 713
1-Methyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 354 MH+ Example 714
2-Methyl-cyclopropane carboxylic acid {6-fluoro-3-[[E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 354 MH+ Example 715
Thiazole-5-carboxylic acid {6-fluoro-3-[{E)-2-(4-f luorophenyl) -vinyl] -1H-indazol-5-yl}-amide MS (ESI) m/z 383 MH+ Example 716
2-Ethyl-N 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-butylamide MS (ESI) m/z 370 MH+ Example 717 2,2-Difluoro-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-
vinyl]-1H-indazol-5-yl}-acetamide MS (ESI) m/z 350 MH+ Example 718
Thiophene-3-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 382 MH+ Example 719
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-2-(pyridin-2-yl)-acetamide MS (ESI) m/z 391 MH+ Example 720
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-2-(pyridin-3-yl)-acetamide MS (ESI) m/z 391 MH+ Example 721
N-{6-Fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-2-(pyridin-4-yl)-acetamide MS (ESI) m/z 391 MH+ Example 722
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl }-2-(thiophen-3-yl)-acetamide MS (ESI) m/z 396 MH+ Example 723
2-Benzo [1, 3] dioxol-5-yl-iV-{ 6-fluoro-3- [ (E) -2- (4-fluorophenyl)-vinyl]-1H- indazol-5-y1}-acetamide MS (ESI) m/z 434 MH+
Example 724
2-Amino-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-acetamide
MS (ESI) m/z 329 MH+
Example 725
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-methylamino-acetamide
MS (ESI) m/z 343 MH+
Example 726
2-Dimethylamino-AM 6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-acetamide
MS (ESI) m/z 357 MH+
Example 727
2-Acetoylamino-N-{6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-acetamide
MS (ESI) m/z 371 MH+
Example 728
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-methoxy-acetamide
MS (ESI) m/z 344 MH+
Example 729
2-Cyano-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-acetamide
MS (ESI) m/z 339 MH+
Example 730
Furan-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 366 MH+ Production example 731 5-Formylfuran-2-carboxylic acid benzyl ester
500 mg of 5-formylfuran-2-carboxylic acid was dissolved in 10 mL of tetrahydrofuran, added with 990 ul of triethylamine, cooled to 0°C, and then added with 374 ul of ethyl chlorof ormate. After stirring at this temperature for 30 minutes, 406 pi of benzylalcohol was added and stirred at room temperature overnight. Adding water to stop the reaction, the solution was extracted with ethyl acetate. The organic phase was washed with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and purified by silica gel column chromatography, to afford 508 mg of the title compound as white crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 5.38 ( 2H, s ), 7.31-7.49 ( 5H, m ), 7.54 ( 1H, dd, J = 1.2, 3.6 Hz ), 7.63 ( 1H, dd, J = 1.2, 3.6 Hz )
Production example 732 Furan-2,5-dicarboxylic acid monobenzyl ester
490 mg of 5-formylfuran-2-carboxylic acid benzyl ester was dissolved in 10 mL of acetonitrile, and added with a solution containing 413mg of sulfamic acid in 5 mL of water. After cooling to 0°C, a solution containing 202 mg of sodium chlorite and 232 mg of potassium dihydrogen phosphate in 5 mL of water was added dropwise. After cooling at this temperature for 30 minutes,
sodium thiosulfate aqueous solution was added at 0°C to stop the reaction, and extracted with a mixture of tetrahydrofuran and ethyl acetate. The organic phase was washed with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off to afford 520 mg of the title compound as white crystals.
1H-NMR (400 MHz, DMSO-D6) δ 5.36 ( 2H, s ), 7.32-7.48 ( 7H, m ) Production example 733 5-Carbamoylfuran-2-carboxylic acid benzyl ester
170 mg of furan-2, 5-dicarboxylic acid monobenzyl ester was dissolved in 5 mL of tetrahydrofuran, and added with 144 µl of triethylamine. After cooling to 0°C, 72.3 µl of ethyl chloroformate was added dropwise and stirred at this temperature for 30 minutes. 1 mL of concentrated ammonia water was added and stirred at room temperature for 10 minutes, and then aqueous ammonium chloride was added to stop the reaction. After extracting the aqueous phase with ethyl acetate, the organic phase was washed with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 185 mg of the title compound as white crystals. 1H-NMR (400 MHz, DMSO-D6) δ 5.35 ( 2H, s ), 7.25 ( 1H, d, J = 1.2 Hz ), 7.35-7.47 ( 6H, m ), 7.67 ( 1H, bs ), 8.04 ( 1H, bs ) Production example 734 5-Methylcarbamoylfuran-2-carboxylic acid benzyl ester
170 mg of furan-2,5-dicarboxylic acid monobenzyl ester obtained by Production example 732 was dissolved in 15 mL of
N,N-dimethylformamide, and this solution was added with 470 ul of diisopropylethylamine, 127 mg of hydroxybenzotriazole, and 414 pi of 2.0M methylamine in tetrahydrofuran, and stirred room temperature for 10 minutes. After adding 265 mg of l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and stirring overnight, ice was added to stop the reaction. The aqueous phase was extracted with ethyl acetate, and the organic phase was washed with water and saturated brine and dried over anhydrous magnesium sulfate. The solvent was distilled off, to afford 128 mg of the title compound as pale yellow crystals. 1H-NMR ( 400 MHz, DMS0-D6 ) δ 2.76 ( 3H, d, J = 4.8 Hz ), 5.36 ( 2H, s ) , 7.20 ( 1H, d, J = 3.6 Hz ) , 7.31-7.4 7 ( 6H, m ) , 8.57 ( 1H, d, J = 4.8 Hz ) Production example 735 5-Dimethylcarbamoylfuran-2-carboxylic acid benzyl ester
In accordance with Production example 734, from 170 mg of furan-2,5-dicarboxylic acid monobenzyl ester obtained by Production example 732 and 414 ul of 2.0 M dimethyl amine in tetrahydrofuran, 139 mg of the title compound was obtained as a yellow oil.
1H-NMR (400 MHz, DMSO-D6) δ 2.98 ( 3H, bs ), 3.18 ( 3H, bs ), 5.35 ( 2H, s ) , 7.12 ( 1H, dd, J = 0.8, 4 . 0 Hz ) , 7.31-7.4 7 ( 6H, m )
Production example 736 5-Carbamoylfuran-2-carboxylie acid
90 mg of 5-carbamoylfuran-2-carboxylic acid benzyl ester
obtained by Production example 733 was suspended in 2 mL of ethanol, added with 9 mg of palladium carbon, and stirred overnight at room temperature under hydrogen atmosphere. The insoluble substances were filtered, and the filtrate was evaporated, and the resultant white solid was washed with 500 µl of diethyl ether, to afford 48 mg of the title compound.
1H-NMR (400 MHz, DMSO-D6) δ 7.12-7.17 (2H, m), 7.54 (1H, bs), 7.89 (1H, bs) Production example 737 5-Methylcarbamoylfuran-2-carboxylic acid
In accordance with the method of Production example 736, from 60 mg of 5-methylcarbamoylfuran-2-carboxylic acid benzyl ester obtained by Production example 734, 48 mg of the title compound was obtained.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 2.75 ( 3H, d, J = 4.8 Hz ), 7.03 ( 1H, bs ), 7.07 ( 1H, bs ), 8.39 ( 1H, bs ) Production example 738 5-Dimethylcarbamoylfuran-2-carboxylic acid
In accordance with the method of Production example 736, from 56 mg of 5-dimethylcarbamoylf uran-2-carboxylic acid benzyl ester obtained by Production example 735, 34 mg of the title compound was obtained.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 2.99 ( 3H, bs ), 3.20 ( 3H, bs ), 7.06 ( 1H, d, J = 3.6 Hz ), 7.20 ( 1H, d, J = 3.6 Hz ) Example 739
In accordance with the method of Example 183, from amines
obtained by Production example 182 and Production example 64 9 and carboxylic acids obtained by Production examples 736-738, the compounds of Examples 740-745 were obtained. Example 740
Furan-2,5-dicarboxylic acid 2-amide 5-{6-fluoro-3-[ (E) -2- (4-f luorophenyl) -vinyl] -1H-indazol-5-yl}-amide MS (ESI) m/z 409 MH+ Example 741
Furan-2,5-dicarboxylic acid 2-({6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide) 5-methylamide MS (ESI) m/z 423 MH+ Example 742
Furan-2,5-dicarboxylic acid 2-dimethylamide 5-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 437 MH+ Example 74 3
Furan-2,5-dicarboxylic acid 2-amide 5-{6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 397 MH+ Example 744
Furan-2,5-dicarboxylic acid 2-{6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazol-5-yl}-amide 5-methylamide MS (ESI) m/z 411 MH+ Example 74 5 Furan-2,5-dicarboxylic acid 2-dimethylamide 5-{6-fluoro-3-
[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 425 MH+ Production example 746 6-Fluoro-3-iodo-5-nitro-lJf-indazole
In accordance with the method of Production example 206, from 5.57 g of 6-fluoro-5-nitro-lH-indazole obtained by Production example 179 and 7.97 g of N-iodosuccinimide, 9.24 g of the title compound was obtained as yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.78 ( 1H, d, J = 11.6 Hz ) , 8.29 ( 1H, d, J = 6.8 Hz ), 14.16 ( 1H, br s ) Production example 747 6-Fluoro-3-iodo-5-nitro-l-trityl-lH-indazole
4.0 g of 6-fluoro-3-iodo-5-nitro-1H-indazole obtained by Production example 746 was dissolved in 150 mL of N,N-dimethylformamide, added with 782 mg of sodium hydride (containing 60%) at 0°C and stirred at this temperature for 10 minutes. After adding 4.35 g of trityl chloride and stirring at room temperature for 5 hours and 30 minutes, ice was added to stop the reaction. The solid precipitated by adding water was collected via glass filter, and washed with water and 50% diethyl ether in hexane. The solvent was evaporated, to afford 5.9 g of the title compound as yellow crystals. 1H-NMR ( 4 00 MHz, DMSO-D6 ) δ 6.18 ( 1H, d, J= 12.8 Hz ) , 7.16-7.19 ( 6H, m ), 7.35-7.40 ( 9H, m ), 8.28 ( 1H, d, J = 7.2 Hz ) Production example 748 3-[(E)-2-(Benzotl,3]dioxol-5-yl)-vinyl]-6-fluoro-5-nitro-l-
trityl-1H-indazole
Under nitrogen atmosphere, 1 g of 6-fluoro-3-iodo-5-nitro-l-trityl-1H-indazole was dissolved in 10 mL of acetonitrile, added successively with 1.35 g of 5-vinyl-benzo[1,3]dioxole, 81. 7 mg of palladium acetate (II), 162 mg of 2-(di-tert-butylphosphino)biphenyl and 2 mL of triethylamine, and stirred at 100°C for 5 hours. After cooling to room temperature, 5 g of silica gel was added, and the solvent was evaporated, andpurif iedby silica gel column chromatography, to obtain 468 mg of the crude product as yellow crystal. This crude product was washed with 50% diethyl ether solution in hexane, and the solvent was evaporated, to afford 143 mg of the title compound as yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 6.06 ( 2H, s ), 6.09 ( 1H, d, J = 12.8 Hz ), 6.93 ( 1H, d, J = 8.4 Hz ), 7.13 ( 1H, d, J = 8.4 Hz ), 7.02-7.23 ( 6H, m ), 7.35-7.39 ( 10H, m ), 7.51 (1H, s ), 7.56 ( 1H, d, J = 16.4 ), 9.14 ( 1H, d, J = 7.2 Hz ) Production example 74 9
3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-6-fluoro-1-trityl-lH-indazole-5-ylamine
123 mg of 3-[(E)-2-(benzo[1,3]dioxol-5-yl)-vinyl]-6-fluoro-5-nitro-l-trityl-1H-indazole was treated in the similar method as described in Production example 182, to afford 95 mg of the title compound as a yellow solid. 1H-NMR ( 400 MHz, CDC13 ) δ 5.97 ( 2H, s ), 6.03 ( 1H, d, J =
12.0 Hz ) , 6.78 ( 1H, d, J = 8.0 Hz ) , 6.94 ( 1H, dd, J = 1.2, 8.0 Hz ), 7.07 ( 1H, d, J = 0.8 Hz ), 7.16-7.33 ( 16H, m ) Example 750
In accordance with Example 183, from 3-[(E)-2-benzo[1,3]dioxol-5-yl-vinyl]-6-fluoro-l-trityl-1H-indazol-5-ylamine obtainedby Production example 749 and various kinds of carboxylic acid, the compounds of Examples 751-756 were obtained. Example 751
N-{3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-6-fluoro-1H-indazol-5-yl}-acetamide MS (ESI) m/z 340 MH+ Example 752
Cyclopropane carboxylic acid {3-[(E)-2-(benzo[1,3]dioxol-5-yl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 366 MH+ Example 753
Furan-2-carboxylic acid {3-[(E)-2-(benzo[1, 3]dioxol-5-yl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 392 MH+ Example 754
N-{3-[ (E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-6-fluoro-lH-indazol-5-yl}-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 422 MH+ Example 755 1-Hydroxy-cyclopropane carboxylic acid { 3- [ (E) -2- (benzo [1,3]-
dioxol-5-yl)-vinyl]-6-fluoro-1H-indazol-5-yl}-amide
MS (ESI) m/z 382 MH+
Example 756
(2S, 4R) -4-Hydroxypyrrolidine-2-carboxylic acid { 3- [ (E) -2-
(benzo[1,3]dioxol-5-yl)-vinyl]-6-fluoro-1H-indazol-5-
yl}-amide
MS (ESI) m/z 411 MH+
Production example 757
l-7Aminomethyl-cyclopropane carboxylic acid
To a solution of 500 mg of 1-cyanocyclopropane carboxylic acid in 50 mL acetic acid was added 50 mg of platinum dioxide, stirred at room temperature for 4 hours under 4 atm. hydrogen atmosphere, and then the platinum dioxide was filtered of f. The solvent was evaporated, to afford 518 mg of the title compound as a yellow oil.
1H-NMR (400 MHz, DMSO-D6) δ 0.58-0.64 (2H, m), 0.93 (2H, dd, J = 3.6, 6.4 Hz), 2.78 (2H, s). Example 758
1-Aminomethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
518 mg of 1-aminomethyl-cyclopropane carboxylic acid obtained by Production example 757 was dissolved in a mixed solvent of 9 mL 1, 4-dioxane/4.5 mL water, added with 4.5 mL of IN sodium hydroxide aqueous solution, stirred for 10 minutes under ice cooling, added with 1.08 g of tert-butyl dicarbonate under ice cooling, and further stirred at room temperature for
21 hours. Then the solvent was evaporated, added with saturated aqueous ammonium chloride under ice cooling, extracted twice with ethyl acetate, and the organic layer was washed with water and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 1-(tert-butoxycarbonylaminomethyl)-cyclopropane carboxylic acid as a colorless oil.
To a solution of 200 mg 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-l-trityl-lH-indazol-5-ylamine obtained by Production example 182 in 3 mL of N,N-dimethylformamide were successively added 126 mg of
1-(tert-butoxycarbonylaminomethyl)-cyclopropane carboxylic acid, 151 mg of diisopropylethylamine, 89.4 mg of 1-hydroxybenztriazole monohydrate and 112 mg of l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (=WSC.HC1), stirred at room temperature for 46 hours, then added with aqueous ammonium chloride, extracted twice with ethyl acetate, an the organic layer was washed successively with water and saturated brine and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant crude product was purified and separated by silica gel column chromatography, to afford (l-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylcarbamoyl}-cyclopropylmethyl)-carbamic acid tert-butyl ester. This was then dissolved in 2 mL of dichloromethane, several drops of triisopropylsilane was added followed by 2 mL of trifluoroacetic acid, and stirred at room temperature for 4.5 hours . Thereafter, saturated aqueous
sodium hydrogen carbonate was added, extracted twice with ethyl acetate, and the organic layer was washed with saturated brine and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the resultant crude product was purified and separated by LC-MS, to afford 46.64 mg of the title compound. 1H-NMR (400 MHz, CD3OD) δ1.24 (2H, dd, J = 5.2, 6.8 Hz), 1.60 (2H, dd, J = 5.2, 6.8 Hz) , 3.19 (2H, s) , 7.08-7.18 (2H, m) , 7.31 (1H, d, J = 10.4 Hz), 7.35 (1H, d, J = 16.8 Hz), 7.46 (1H, d, J = 16.8 Hz), 7.60-7.68 (2H, m), 8.14 (1H, d, J = 6.8 Hz). MS (ESI) m/z 369 MH+ Example 759
1-(Acetylamino-methyl)-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
To a solution of 10 mg of 1-aminomethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-2H-indazol-5-yl}-amide obtained by Example 758 in 0.3 mL of N,N-dimethylformamide was added 2 . 9 mg of acetic anhydride, and stirred at room temperature for 38 hours under nitrogen atmosphere, and separated and purified by LC-MS, to afford 3. 66 mg of the title compound.
1H-NMR (400 MHz, CD3OD)δ0.98 (2H, dd, J = 4.4, 7.2 Hz), 1.32 (2H, dd, J=4.4, 7.2Hz), 2.03 (3H, s), 3.60 (2H, s) , 7.06-7.16 (2H, m), 7.30 (1H, d, J = 10.0 Hz), 7.35 (1H, d, J = 16.8 Hz), 7.46 (1H, d, J = 16.8 Hz), 7.60-7.69 (2H, m), 8.30 (1H, d, J =7.2 Hz).
MS (ESI) m/z 411 MH+
Production example 760
3-[[E)-2-(4-Fluorophenyl)-vinyl]-4-[3-(tetrahydropyran-2-
yloxy)-propoxy]-lH-indazole-1,5-dicarboxylic acid
1-tert-butyl ester 5-ethyl ester
In accordance with the method of Production example 347, from 200 mg of 3-[ (E) -2- (4-fluorophenyl)-vinyl]-4-hydroxy-lH-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester obtained by Production example 346 and 1.05 g of 2-(3-bromopropoxy)-tetrahydropyran, 267 mg of the title compound was obtained as a colorless oil.
1H-NMR (4 00 MHz, CDCl3) δ1. 23-2. 33 (8H, m) , 1.44 (3H, t, J =6.8 Hz) , 1.75 (9H, s) , 3.36-3.4 4 (1H, m) , 3.52-3.60 (1H, m) , 3.68-3.7 6 (1H, m), 3.85-3.91 (1H, m), 4.18-4.29 (2H, m), 4.43 (2H, q, J = 6.8 Hz), 4.49 (1H, t, J = 4.0Hz), 7.05-7.12 (2H, m) , 7.52-7.62 (3H, m) , 7.80 (1H, d, J = 16.4 Hz), 7.87 (1H, d, J = 8.8 Hz), 7.99 (1H, d, J = 8.8 Hz). Example 7 61
3- [ (E)-2-(4-Fluorophenyl)-vinyl]-4-(3-hydroxypropoxy) -1H-indazole-5-carboxylic acid
In accordance with the methods of Example 16 and Example
350, from 267 mg of 3- [ (E) -2-(4-fluorophenyl)-vinyl]-4-[3-
(tetrahydropyran-2-yloxy)-propoxy]-lH-indazole-1,5-dicarbox
ylicacid 1-tert-butyl ester 5-ethyl ester obtained by Production
example 760, 83.1 mg of the title compound was obtained as flesh
color crude crystals.
1H-NMR (400 MHz, CD3OD) δ2. 06-2.18 (2H, m) , 3.82 (2H, t, J =6.0 Hz), 4.2 6 (2H, t, J = 6.4 Hz), 7.10-7.17 (2H, m), 7.25 (1H, d, J = 8.4 Hz), 7.54 (1H, d, J = 16.0 Hz), 7.59-7.67 (2H, m) , 7.63 (1H, d, J = 16.0 Hz), 7.84 (1H, d, J = 8.4 Hz). MS (ESI) m/z 355 (M-H)~ Production example 7 62
4-(2-Carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester
In accordance with the method of Production example 347, from 120 mg of 3-[(E) -2-(4-fluorophenyl)-vinyl]-4-hydroxy-li?-indazole-l, 5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester obtained by Production example 346 and 427.1 mg of 3-bromopropionamide, 110 mg of the title compound was obtained as colorless crystals.
1H-NMR (400 MHz, CDCl3)δ1.45 (3H, t, J =7.2 Hz), 1.75 (9H, s) , 2.84 (2H, t, J = 5.6 Hz), 4.37 (2H, t, J = 5.6 Hz), 4.42 (2H, q, J = 7.2 Hz), 7.06-7.15 (2H, m), 7.50 (1H, d, J = 16.4 Hz), 7.58-7.66 (2H, m), 7.80 (1H, d, J = 16.4 Hz), 7.94 (1H, d, J = 8.8 Hz), 8.07 (1H, d, J = 8.8 Hz). Example 7 63
4-(2-Carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester
In accordance with the method of Example 16, from 110 mg of 4-(2-carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-1,5-dicarboxylic acid
1-tert-butyl ester 5-ethyl ester obtained by Production example 762, 78 mg of the title compound was obtained as colorless crude crystals.
1H-NMR (400 MHz, CD3OD) δ1.42 (3H, t, J = 6.8 Hz), 2.83 (2H, t, J = 6.0 Hz), 4.39 (2H, t, J = 6.0 Hz), 4.40 (2H, q, J = 6.8 Hz), 7.0 9-7.18 (2H, m), 7.28 (1H, d, J= 8.8 Hz), 7.53 (1H, d, J = 16.4 Hz) , 7.62 (1H, d, J = 16.4 Hz) , 7.66-7.74 (2H, m) , 7.85 (1H, d, J = 8.8 Hz). MS (ESI) m/z 396 (M-H)~ Example 7 64
4-(2-Carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-i ndazole-5-carboxylic acid
85.3 mg of 4-(2-carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-i ndazole-5-carboxylic acid ethyl ester obtained by Example 763 was dissolved in 3 mL of concentrated sulfuric acid, stirred at 35°C for 16 hours, and then added with ice under ice cooling. The precipitated crystals were collected by filtration, washed with water, and then dried under reduced pressure, to afford 43.7 mg of the title compound as yellow crude crystals. 1H-NMR (400 MHz, CD3OD)δ2.85 (2H, t, J = 6.0 Hz), 4.41 (2H, t, J = 6.0 Hz) , 7.06-7.18 (2H, m), 7.28 (1H, d, J=8.8Hz), 7.44-7.76 (2H, m), 7.51 (1H, d, J = 16.4 Hz), 7.60 (1H, d, J = 16.4 Hz) , 7.92 (1H, d, J = 8.8 Hz). MS (ESI) m/z 370 MH+ Production example 7 65
4-(2-Bromoethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester
In accordance with the method of Production example 347, from 3.34 g of 3- [ (E)-2- (4-fluorophenyl) -vinyl] -4-hydroxy-2H-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester obtained by Production example 346 and 14.7 g of 1,2-dibromoethane, 3.20 g of the title compound was obtained as a colorless foam.
1H-NMR (4 00 MHz, CDCl3)δ1.4 4 (3H, t, J=7.2Hz), 1.75 (9H, s), 3.78 (2H, t, J = 5.6 Hz), 4.42 (2H, q, J = 7.2 Hz), 4.46 (2H, t, J = 5.6 Hz) , 7.04-7.13 (2H, m) , 7.56-7.68 (2H, m) , 7.64 (1H, d, J = 16.4 Hz), 7.81 (1H, d, J - 16.4 Hz), 7.91 (1H, d, J = 8.8 Hz), 8.03 (1H, d, J = 8.8 Hz). Example 7 66
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-[2-(morpholin-4-yl)ethoxy]-lH-indazole-5-carboxylic acid ethyl ester
100 mg of 4-(2-bromoethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-1,5-dicarboxylic acid 1-tert-butyl ester 5-ethyl ester obtained by Production example 765 was dissolved in 1 mL of W,iV-dimethylformamide, added with 40.8 mg of morpholine, and stirred at room temperature for 14 hours and 30 minutes, followed by stirring at 50°C for 3 hours and further at 80°C for 1 hour. Then adding water and extracting twice with ethyl acetate, the organic layer was washed successively with water and saturated brine and dried over
anhydrous magnesium sulfate. The solvent was evaporated, to
afford 81.9 mg of the title compound.
1H-NMR (400 MHz, CD3OD) δ1.41 (3H, t, J = 7.2 Hz), 2.45 (4H, bs),
2.86 (2H, t, J = 6.0 Hz), 3.45-3.70 (4H, m), 4.27 (2H, t, J =
6.0 Hz), 4.39 (2H, q, J = 7.2 Hz), 7.07-7.18 (2H, m), 7.27 (1H,
d, J = 8.4 Hz), 7.52 (1H, d, J = 16.8 Hz), 7.58-7.70 (3H, m),
7.83 (1H, d, J = 8.4 Hz).
Example 7 67
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-[2-(morpholin-4-
yl) ethoxy] -lH-indazole-5-carboxylic acid
In accordance with the method of Example 350, from 81.9 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-[2-(morpholin-4-yl) ethoxy] -1H-indazole-5-carboxylic acid ethyl ester obtained by Example 766, 64.1 mg of the title compound was obtained. MS (ESI) m/z 412 MH+ Example 7 68
3-[(E)-2-(4-Fluorophenyl) -vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [ (lH-pyrrol-2-yl)-methyl]-amide
To a solution of lH-pyrrole-2-carboaldehyde in 31.5 mL of methanol, 8.1 g ammonium acetate and 462 mg of sodium cyano borohydride were added, and under nitrogen atmosphere stirred at room temperature for 27 hours. Then, concentrated hydrochloric acid was added until pH decreased to not more than 2, and the solvent was evaporated. After dissolving the residue in water, the solution was extracted twice with diethyl ether. Then, the aqueous phase was added with potassium hydroxide until
pH reached to not less than 10, extracted twice with ethyl acetate, and dried over anhydrous magnesium sulfate. The solvent was evaporated, followed by purification and separation by NH silica gel column chromatography, to afford 475 mg of C-(lH-pyrrol-2-yl)-methylamine as a brown oil.
In accordance with the method of Example 102, from 10 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234 and 9.2 mg of C- (lH-pyrrol-2-yl) -methylamine, 0 . 98 mg of the title compound was obtained.
1H-NMR (400 MHz, CD3OD)δ3.84 (3H, s) , 4.58 (2H, s), 6.03 (1H, dd, J = 2.8, 5.6 Hz), 6.09-6.13 (1H, m), 6.67-6.72 (1H, m), 7.06-7.18 (2H, m), 7.32 (1H, d, J = 8.4 Hz), 7.52 (1H, d, J = 16.4 Hz), 7.56 (1H, d, J = 16.4 Hz), 7.56-7.66 (2H, m) , 7.82 (1H, d, J = 8.4 Hz). MS (ESI) m/z 391 MH+ Production example 7 69
[Oxazol-2-yl-(toluene-4-sulfonyl)-methyl]-carbamic acid tert-butyl ester
300 mg of oxazole 2-carboaldehyde was dissolved in a mixed solvent of 3 mL of methanol and 6 mL of water, added successively with 363 mg of tert-butyl carbamate, 552 mg of sodium p-toluenesulfonate and 0.76 mL of formic acid and stirred at room temperature for 16 hours and 30 minutes. The precipitated crystals were collected by filtration, washed with water, and dried under reduced pressure, to afford 247 mg of the title
compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ1.23 (9H, s) , 2.40 (3H, s), 6.12 (1H,
d, J = 10.0 Hz), 7.35 (1H, s), 7.46 (2H, d, J = 7.6 Hz), 7.71
(2H, d, J = 7.6 Hz) , 8.28 ( 1H, s ) , 8.8 6 (1H, d, J = 10.0 Hz).
Production example 770
Oxazole-2-ylmethyl-carbamic acid tert-butyl ester
To 4 mL of tetrahydrofuran was added 51.4 mg of sodium borohydride, and added with 240 mg of [oxazole-2-yl-(toluene -4-sulfonyl)-methyl]-carbamic acid tert-butyl ester obtained by Production example 7 69 over 13 minutes at room temperature under stirring. Thereafter, the solution was stirred for another 2 hours and 30 minutes under room temperature, added with saturated aqueous ammonium chloride under ice cooling, and stirred for another 30 minutes under ice cooling. Thereafter, water was added, extracted twice with diethyl ether and dried over anhydrous magnesium sulfate. The solvent was evaporated, followed by purification and separation by silica gel column chromatography, to afford 72.3 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ1.39 (9H, s) , 4.44 (1H, d, J = 5.6 Hz), 7.51 (1H, s), 7.61 (1H, t, J = 5.6 Hz), 8.38 (1H, s). Example 771
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (oxazol-2-ylmethyl)-amide
In accordance with the method of Example 16, from 36 mg oxazol-2-ylmethyl-carbamic acid tert-butyl ester obtained by
Production example 770, C-(oxazol-2-yl)-methylamine was obtained as transparent oil. From this and 10 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234, 3.82 mg of the title compound was obtained in accordance with the method of Example 102.
1H-NMR (400 MHz, CD3OD)δ4.00 (3H, s), 4.78 (2H, s) , 7.09-7.19 (2H, m), 7.16 (1H, d, J = 0.8 Hz), 7.43 (1H, d, J = 8.8 Hz), 7.55 (1H, d, J = 16.4 Hz), 7.60 (1H, d, J = 16.4 Hz), 7.59-7.68 (2H, m) , 7.85 (1H, d, J = 8.8 Hz), 7.90 (1H, d, J = 0.8 Hz). MS (ESI) m/z 393 MH+ Production example 772
Acetic acid 5-(tert-butoxycarbonylamino-methyl)-furan-2-ylmethyl ester
In accordance with the methods of Production example 769 and Production example 770, from 1 g of acetic acid 5-formyl-furan-2-ylmethyl ester, 443 mg of the title compound was obtained as a colorless oil.
1H-NMR (400 MHz, DMSO-D6) δ1.36 (9H, s), 2.00 (3H, s), 4.07 (2H, d, J = 5.2 Hz), 4.95 (2H, s), 6.13 (1H, d, J = 2.8 Hz), 6.40 (1H, d, J = 2.8 Hz), 7.32 (1H, bs) . Production example 773
(5-Hydroxymethyl-furan-2-ylmethyl)-carbamic acid tert-butyl ester
416 mg of acetic acid 5-(tert-butoxycarbonylamino-methyl)-furan-2-yl methyl ester
was dissolved in a mixed solvent of 8 mL of methanol and 8 mL of water, added with 639 mg of potassium carbonate, and stirred at room temperature for 30 minutes . Then, the solution was added with saturated aqueous ammonium chloride, extracted twice with ethyl acetate, the organic layer washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 363 mg of the title compound as a colorless oil.
1H-NMR (400 MHz, DMSO-D6) δ 1.36 (9H, s) , 4.05 (2H, d, J = 5.6 Hz), 4.30 (2H, d, J = 5.6 Hz), 5.13 (1H, t, J = 5.6 Hz), 6.06 (1H, d, J = 2.8 Hz), 6.15 (1H, d, J = 2.8 Hz), 7.28 (1H, t, J = 5.6 Hz) .
Production example 774
(5-Azidomethyl-furan-2-ylmethyl)-carbamic acid tert-butyl ester
99.4 mg of (5-hydroxymethyl-furan-2-ylmethyl)-carbamic acid tert-butyl ester was added to 4.5 mL of toluene, added successively with 83.7 mg of 1,8-diazabicyclo [5,4,0]undec-7-ene and 151.6 mg of diphenylphosphoryl azide, and stirred at room temperature under nitrogen atmosphere for 1 hour and 45 minutes . Then the solvent was evaporated, followed by purification and separation by silica gel column chromatography, to afford 88.6 mg of the title compound as a colorless oil.
1H-NMR (400 MHz, DMSO-D6) δ 1.36 (9H, s), 4.07 (2H, d, J = 5.2 Hz), 4.39 (2H, s), 6.14 (1H, t, J = 2.8 Hz), 6.38 (1H, d, J =
2.8 Hz), 7.32 (1H, t, J = 5.2 Hz).
Production example 775
(5-Aminomethyl-furan-2-ylmethyl)-carbamic acid tert-butyl
ester
To a solution of 88.6 mg of (5-azidomethyl-furan-2-ylmethyl)-carbamic acid tert-butyl ester in 3 mL of ethanol was added 30 mg of Lindlar catalyst, stirred at room temperature under hydrogen atmosphere for 15 hours, the catalyst filtered off, and the solvent was evaporated, to afford 81 mg of the title compound as a colorless oil. 1H-NMR (400 MHz, CD3OD) δ 1.43 (9H, s), 3.71 (2H, s) , 4.15 (2H, s) , 6.05-6.20 (2H, m) . MS (ESI) m/z 225 (M-H)-Example 77 6
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (5-aminomethyl-furan-2-ylmethyl)-amide
In accordance with the methods of Example 102 and Example 16, from 19 mg (5-aminomethyl-furan-2-ylmethyl)-carbamic acid tert-butyl ester obtained by Production example 775 and 17.5 mg of 3-[[E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234, 4.26 mg of the title compound was obtained.
1H-NMR (400 MHz, CD3OD) δ 3.92 (3H, s) , 4.15 (2H, s), 4.65 (2H, s), 6.40 (1H, d, J=3.6Hz), 6.51 (1H, d, J=3.6Hz), 7.08-7.18 (2H, m) , 7.34 (1H, d, J = 8.4 Hz), 7.53 (1H, d, J = 16.4 Hz), 7.58 (1H, d, J = 16.4 Hz), 7.58-7.66 (2H, m), 7.81 (1H, d, J
= 8.4 Hz).
MS (ESI) m/z 421 MH+
Example 7 77
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (5-methylaminomethyl-furan-2-ylmethyl)-amide
To a solution of 15 mg of acetic acid 5-(tert-butoxycarbonylamino-methyl)-furan-2-ylmethylester obtained by Production example 772 in 0.5 mL of N,N-dimethylformamide were added 31. 8 mg of iodomethane and 2 . 5 mg of sodium hydride under ice cooling, and stirred for another 2 hours under ice cooling. Then saturated aqueous ammonium chloride was added under ice cooling, extracted twice with diethyl ether, the organic layer washed with water, and dried over anhydrous magnesium sulfate . The solvent was evaporaeted, to afford acetic acid 5-[(tert-butoxycarbonyl-methyl-amino)-methyl] -furan-2-ylmethyl ester. This was then subjected to the reactions similar to those described in Production example 773, Production example 774 and Production example 775, to afford (5-aminomethyl-furan-2-ylmethyl)-methyl-carbamic acid tert-butyl ester.
This was then reacted with 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid obtained by Example 234 in accordance with the methods of Example 102 and Example 16, followed by separation and purification by LC-MS, to afford 0.58 mg of the title compound. 1H-NMR (400MHz, CD3OD) δ 2.70 (3H,s), 3.93 (3H, s), 4.24 (2H,
s), 4.66 (2H, s), 6.43 (1H, d, J = 3.2 Hz), 6.60 (1H, d, J =
3.2 Hz) , 7.10-7.18 (2H, m) , 7.35 (1H, d, J = 8.8 Hz) , 7.53 (1H,
d, J = 16.4 Hz) , 7.59 (1H, d, J = 16.4 Hz) , 7.60-7.68 (2H, m) ,
7.81 (1H, d, J = 8.8 Hz).
MS (ESI) m/z 435 MH+
Example 778
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (5-dimethylaminomethyl-furan-2-
ylmethyl)-amide
10 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (5-aminomethyl-furan-2-ylmethyl)-amide obtained by Example 776 was dissolved in a mixed solvent of 0 . 25 mL methanol/ 0 . 25 mL tetrahydrof uran, added with 7.2 mg of 37% formaldehyde aqueous solution, 6 µl of acetic acid, and3. 8mg of sodium cyanoborohydride, andstirred at room temperature under nitrogen atmosphere for 18 hours . Then the solvent was evaporated, followed by separation and purification by LC-MS, to afford 0.60 mg of the title compound. 1H-NMR (400 MHz, CD3OD) δ 2.87 (6H,s), 3.94 (3H, s) , 4.38 (2H, s), 4.67 (2H, bs), 6.47 (1H, d, J = 2.8 Hz), 6.69 (1H, d, J = 2.8 Hz) , 7.10-7.20 (2H, m) , 7.35 (1H, d, J = 8.8 Hz) , 7.53 (1H, d, J = 16.8 Hz), 7.59 (1H, d, J = 16.8 Hz), 7.60-7.68 (2H, m) , 7.80 (1H, d, J = 8.8 Hz). MS (ESI) m/z 450 MH+ Example 77 9 3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-

carboxylic acid (5-hydroxymethyl-furan-2-ylmethyl)-amide
In accordance with the method of Example 16, from acetic acid 5-(tert-butoxycarbonylamino-methyl)-furan-2-ylmethyl ester obtained by Production example 772, acetic acid 5-aminomethyl-furan-2-ylmethyl ester was obtained.
This compound and 17.5 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234 were amidated in accordance with Example 102, followed by hydrolysis of acetate in accordance with the method of Production example 773. Then the resultant product was separated and purified by LC-MS, to afford 1.95 mg of the title compound.
1H-NMR (400 MHz, CD3OD) δ 3.91 (3H, s), 4.49 (2H, s) , 4.63 (2H, s), 6.27 (1H, d, J = 3.2Hz), 6.31 (1H, d, J = 3.2Hz), 7.08-7.18 (2H, m), 7.33 (1H, d, J = 8.8 Hz), 7.53 (1H, d, J = 16.4 Hz), 7.58 (1H, d, J = 16.4 Hz), 7.59-7.67 (2H, m), 7.79 (1H, d, J =8.8 Hz).
MS (ESI) m/z 422 MH+ Example 780
(S)-({3-[[E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carbonyl}-amino)-phenyl-acetic acid
In accordance with the methods of Example 102 and Example 16, from 40 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234 and (S)-amino-phenyl-acetic acid tert-butyl ester, 110 mg of the title compound was obtained as a yellow oil.
MS (ESI) m/z 446 MH+ Example 781
From carboxylic acids obtained by Example 7 61, Example 7 64, Example 234 and Example 780 and various kinds of amine, the compounds of Examples 782-794 were obtained in accordance with the method of Example 102. Example 7 82
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-(3-hydroxypropoxy)-1H-indazole-5-carboxylic acid [(1S)-l-hydroxymethyl-2-methyl-propyl]-amide MS (ESI) m/z 443 MH+ Example 7 83
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-(3-hydroxypropoxy)-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 396 MH+ Example 784
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-(3-hydroxypropoxy)-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 436 MH+ Example 7 85
3- [ {E) -2- (4-Fluorophenyl)-vinyl]-4-(3-hydroxypropoxy)-1H-indazole-5-carboxylic acid [(1S)-2-hydroxy-l-phenyl-ethyl]-amide MS (ESI) m/z 477 MH+ Example 786 3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-(3-hydroxypropoxy)-1H-
indazole-5-carboxylic acid [(1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 427 MH+
Example 787
4-(2-Carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazole-5-carboxylic acid [(15)-l-hydroxymethyl-2-
methyl-propyl]-amide
MS (ESI) m/z 456 MH+
Example 788
4-(2-Carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 409 MH+
Example 78 9
4-(2-Carbamoylethoxy)-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 449 MH+
Example 7 90
4-(2-Carbamoylethoxy)-3-[(E) -2-(4-fluorophenyl)-vinyl]-1H-
indazole-5-carboxylic acid [(1S)-2-hydroxy-l-
phenyl-ethyl]-amide
MS (ESI) m/z 490 MH+
Example 7 91
4-(2-Carbamoylethoxy)-3-[(E) -2-(4-fluorophenyl)-vinyl]-1H-
indazole-5-carboxylic acid [(1S)-1-carbamoyl-ethyl]-amide
MS (ESI) m/z 440 MH+
Example 792
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid cyclopropylmethyl-amide
MS (ESI) m/z 366 MH+
Example 7 93
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid [(3R)-2-oxo-tetrahydrofuran-3-yl]-amide
MS (ESI) m/z 396 MH+
Example 7 94
3-[[E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(S)-cyclopropylcarbamoyl-phenyl-methyl]-
amide
MS (ESI) m/z 486 MH+
Example 795
To a solution of either carboxylic acid obtained by Example 7 67, Example 234 and Example 780 in 1.5 mL of tetrahydrofuran were successively added 6 equivalents of triethylamine and 2.5 equivalents of ethyl chlorof ormate under ice cooling, and stirred under ice cooling for 1 hour. Then an excess amount of various kinds of amine was added and stirred at room temperature overnight. The solvent was distilled off, and the resultant residue was separated and purified by LC-MS, to afford the compounds of Examples 796-799. Example 7 96
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-[2-(morpholin-4-yl)ethoxy]-1H-indazole-5-carboxylic acid amide MS (ESI) m/z 411 MH+ Example 7 97
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-[2-(morpholin-4-yl)-
ethoxy]-lH-indazole-5-carboxylic acid methylamide
MS (ESI) m/z 425 MH+
Example 7 98
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid methylamide
MS (ESI) m/z 326 MH+
Example 7 99
3-[2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(S)-dimethylcarbamoyl-phenyl-methyl]-amide
MS (ESI) m/z 474 MH+
Production example 800
1-Benzyloxymethyl-cyclopropylamine
The title compound was prepared in accordance with the known method (J.Org.Chem.2002, 61, 3965. ) . Production example 801 1-(2-Benzyloxy-ethyl)-cyclopropylamine
The title compound was prepared in accordance with the known method (J. Org. Chem. 2002, 67,3965. ) . Production example 802 l-Azido-2-methyl-propan-2-ol
3. 6 g of isobutylene oxide was dissolved in 100 mL of water, added with 18.2 g of cetyltrimethyl ammonium bromide and 16.3 g of sodium azide, and stirred at 30°C overnight. The solution was extracted with 50% tetrahydrofuran solution in ethyl acetate, the organic phase was washed with water and saturated brine,
and dried over anhydrous magnesium sulfate. The solvent was evaporated, followed by purification by silica gel column chromatography, and the solvent was evaporated at room temperature, to afford 6.44 g of the title compound as a mixture with ethyl acetate.
1H-NMR ( 400 MHz, CDCl3 ) δ1.26 ( 6H, s ), 2.04 ( 2H, s ) Production example 803 l-Amino-2-methyl-propan-2-ol
3.22 g of l-azido-2-methyl-propan-2-ol obtained by Production example 802 was dissolved in 50 mL of ethanol, added with 30 mg of palladium carbon, and stirred overnight at room temperature under hydrogen atmosphere. The insoluble substances were filtered off through Celite, and the solvent was evaporated, toaffordl.5goft he title compound as a colorless oil substance.
1H-NMR ( 400 MHz, DMSO-D6 )δ1.16 ( 6H, s ), 2.71 ( 2H, dd, J = 6, 11.6 Hz ) Production example 804 l-Amino-3-methyl-buthan-3-ol hydrochloride
To 3 g of 3-hydroxy-3-methylbutyronitrile, 10mL of ethanol, 5 mL of concentrated hydrochloric acid and 100 mg of platinum dioxide were added, and under 5-atom hydrogen atmosphere, the solution was stirred at room temperature for 5 hours. The insoluble substances were filtered off through Celite, and the solvent evaporated, to afford 3.5 g of the title compound as a dark brown solid.
1H-NMR ( 400 MHz, DMSO-D6 ) δ1.12 ( 6H, s ), 1.65 ( 2H, t, J = 8.0 Hz ), 2.82-2.87 ( 2H, m ) Production example 805 2-Amino-N,N-dimethyl-acetamide
10 g of glycine ethyl ester hydrochloride was suspended in 200 mL of dichloromethane, added with 22 mL of triethylamine, cooled to 0CC, and added with 11.3 mL of benzylchloroformate. The suspension was allowed to warm to room temperature, stirred for4days, and added with water to stop the reaction. Extracting with ethyl acetate, the organic layer was washed with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and 12.2 g of the resultant white crystal was suspended in 200 mL of ethanol, added with 100 mL of 1N sodium hydroxide aqueous solution, and stirred overnight at room temperature. The organic solvent was evaporated, and the residual aqueous phase was washed twice with 100 mL of ether. The aqueous phase was made to pH 2 by using 5N hydrochloric acid, and extracted with ethyl acetate. The organic phase was washed with water and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated, to afford 4.82 g of white crystals. 497 mg of this white crystals were dissolved in 20 mL of DMF, added with 1.62 mL of diisopropylethylamine, 1.4 mL of dimethyl amine in 2.0 M tetrahydrofuran and 437 mg of hydroxybenzotriazole, and stirred at room temperature for 10 minutes. After cooling to 0°C, 912 mg of WSC hydrochloride was added and stirred overnight at room temperature. Water was
added to stop the reaction, the solution was extracted with ethyl acetate, and the organic phase washed with water and saturated brine and dried over anhydrous magnesium sulfate. The solvent was evaporated, followed by purification by silica gel column chromatography, to afford 402 mg of a colorless oil substance. This was then dissolved in 5 mL of ethanol, added with 40 mg of palladium carbon, and stirred overnight at room temperature under hydrogen atmosphere. The insoluble substances were filtered off through Celite, and the filtrate was evaporated, to afford 113 mg of the title compound.
1H-NMR ( 400 MHz, CD3OD )δ2.95 ( 3H, s ), 2.97 ( 3H, s ), 3.44 ( 2H, s ) Production example 806
The compounds of Production example 8 07 to Production example 811 wereprepared in accordance withmethod of Production example 805. Production example 807 3-Amino-N-methyl-propionamide
1H-NMR ( 400 MHz, CD3OD ) δ2.39 ( 2H, t, J = 6.8 Hz ) , 2.71 ( 3H, s ), 3.46 ( 2H, t, J = 6.4 Hz ) Production example 808 3-Amino-N,N-dimethyl-propionamide
1H-NMR ( 400 MHz, CD3OD ) δ 2.60 ( 2H, t, J = 6.8 Hz ) , 2.93 ( 3H, s ), 3.03 ( 3H, s ), 3.47 ( 2H, t, J = 6.8 Hz ) Production example 809 4-Amino-butylamide
1H-NMR ( 400 MHz, CD3OD )δ1.80 ( 2H, quint, J = 7.2 Hz ) , 2.24 ( 2H, t, J = 7.2 Hz ), 3.25 ( 2H, t, J = 7.2 Hz ) Production example 810 4-Amino-N-methyl-butylamide
1H-NMR ( 400 MHz, CD3OD ) δ1.79 ( 2H, quint, J = 7.2 Hz ) , 2.21 ( 2H, t, J = 7.2 Hz ), 2.71 ( 3H, s ), 3.23 ( 2H, t, J = 7.2 Hz )
Production example 811 4-Amino-N,N-dimethyl-butylamide
1H-NMR ( 400 MHz, CD3OD )δ1.79 ( 2H, quint, J = 7.2 Hz ), 2.41 ( 2H, t, J = 7.2 Hz ) , 2.92 ( 3H, s ) , 3.05 ( 3H, s ) , 3.25 ( 2H, t, J = 7.2 Hz ) Example 812
In accordance with the method of Example 102, from 3-[(E) -2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-ca rboxylic acid obtained by Example 234 or commercially available amine, or various kinds of amine obtained by Production example 800, 801, 803, 804, 805, 807-811, the compounds of Examples 813-887 were obtained. Example 813
3-[(E)~2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (1-benzyloxymethyl-cyclopropyl)-amide MS (ESI) m/z 472 MH+ Example 814
3-[(E)~2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid [1-(2-benzyloxyethyl)-cyclopropyl]-amide
MS (ESI) m/z 486 MH+
Example 815
3-[ (E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (1-hydroxymethyl-cyclopentyl)-amide
MS (ESI) m/z 410 MH+
Example 816
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-lH-indazole-5-
carboxylic acid (2-hydroxy-l,1-dimethyl-ethyl)-amide
MS (ESI) m/z 384 MH+
Example 817
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [{1R)-l-hydroxymethyl-2-methyl-propyl]-amide
MS (ESI) m/z 398 MH+
Example 818
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(1R)-2-hydroxy-l-phenyl-ethyl]-amide
MS (ESI) m/z 432 MH+
Example 819
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-hydroxy-l-(pyridin-3-yl)-ethyl]-amide
MS (ESI) m/z 433 MH+
Example 820
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (2-hydroxyethyl)-amide
MS (ESI) m/z 356 MH+
Example 821
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (2-hydroxy-2-methyl-propyl)-amide
MS (ESI) m/z 384 MH+
Example 822
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (3-hydroxy-3-methyl-butyl)-amide
MS (ESI) m/z 398 MH+
Example 823
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(2S)-2-hydroxy-2-phenyl-ethyl]-amide
MS (ESI) m/z 432 MH+
Example 824
3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(2R)-2-hydroxy-2-phenyl-ethyl]-amide
MS (ESI) m/z 432 MH+
Example 825
3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(2S)-2-hydroxypropyl]-amide
MS (ESI) m/z 370 MH+
Example 826
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(2R)-2-hydroxypropyl]-amide
MS (ESI) m/z 370 MH+
Example 827
3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (3-amino-propyl)-amide
MS (ESI) m/z 369 MH+
Example 828
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (3-methylamino-propyl)-amide
MS (ESI) m/z 383 MH+
Example 829
3- [ (E) -2- (4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (3-ethylamino-propyl)-amide
MS (ESI) m/z 397 MH+
Example 830
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5~
carboxylic acid (3-dimethylamino-propyl)-amide
MS (ESI) m/z 397 MH+
Example 8 31
3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [3-(2-oxo-pyrrolidin-l-yl)-propyl]-amide
MS (ESI) m/z 437 MH+
Example 832
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [3-(morpholin-4-yl)-propyl]-amide
MS (ESI) m/z 439 MH+
Example 833
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [3-(lH-imidazol-1-yl)-propyl]-amide
MS (ESI) m/z 420 MH+
Example 834
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-(lH-imidazol-4-yl)-ethyl]-amide
MS (ESI) m/z 406 MH+
Example 835
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid (4-amino-cyclohexyl)-amide
MS (ESI) m/z 409 MH+
Example 836
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(3R)-pyrrolidin-3-yl]-amide
MS (ESI) m/z 381 MH+
Example 837
3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [(3S)-pyrrolidin-3-yl]-amide
MS (ESI) m/z 381 MH+
Example 838
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (piperidin-4-ylmethyl)-amide
MS (ESI) m/z 409 MH+
Example 839
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (piperidin-4-yl)-amide
MS (ESI) m/z 395 MH+
Example 84 0
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid carbamoylmethyl-amide
MS (ESI) m/z 369 MH+
Example 841
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid methylcarbamoylmethyl-amide
MS (ESI) m/z 383 MH+
Example 8 42
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid dimethylcarbamoylmethyl-amide
MS (ESI) m/z 397 MH+
Example 843
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (2-carbamoyl-ethyl)-amide
MS (ESI) m/z 383 MH+
Example 84 5
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (2-methylcarbamoyl-ethyl)-amide
MS (ESI) m/z 397 MH+
Example 84 6
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (2-dimethylcarbamoyl-ethyl)-amide
MS (ESI) m/z 411 MH+
Example 84 7
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (3-carbamoyl-propyl)-amide
MS (ESI) m/z 397 MH+
Example 84 8
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (3-methylcarbamoyl-propyl)-amide
MS (ESI) m/z 411 MH+
Example 84 9
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (3-dimethylcarbamoyl-propyl)-amide
MS (ESI) m/z 425 MH+
Example 850
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(1S)-1-methylcarbamoyl-ethyl]-amide
MS (ESI) m/z 397 MH+
Example 851
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (2-oxo-azepan-3-yl)-amide
MS (ESI) m/z 423 MH+
Example 852
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (pyridin-2-ylmethyl)-amide
MS (ESI) m/z 403 MH+
Example 853
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI) m/z 403 MH+
Example 854
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (pyridin-4-ylmethyl)-amide
MS (ESI) m/z 403 MH+
Example 855
3- [ (E) -2- (4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid benzylamide
MS (ESI) m/z 402 MH+
Example 856
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-(pyridin-2-yl)ethyl]-amide
MS (ESI) m/z 417 MH+
Example 857
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-(pyridin-3-yl)ethyl]-amide
MS (ESI) m/z 417 MH+
Example 8 58
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-(pyridin-4-yl)ethyl]-amide
MS (ESI) m/z 417 MH+
Example 859
3- [ (E) -2- (4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (pyrazin-2-ylmethyl)-amide
MS (ESI) m/z 404 MH+
Example 8 60
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-(thiophen-2-yl)-ethyl]-amide
MS (ESI) m/z 422 MH+
Example 8 61
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 408 MH+
Example 8 62
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid cyclobutylamide
MS (ESI) m/z 366 MH+
Example 8 63
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid cyclopentylamide
MS (ESI) m/z 380 MH+
Example 8 64
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid allylamide
MS (ESI) m/z 352 MH+
Example 8 65
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid propan-2-ynylamide
MS (ESI) m/z 352 MH+
Example 8 66
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-lH-indazole-5-
carboxylic acid cyanomethyl-amide
MS (ESI) m/z 351 MH+
Example 8 67
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (thiophen-3-ylmethyl)-amide
MS (ESI) m/z 408 MH+
Example 8 68
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid (5-methyl-furan-2-ylmethyl)-amide
MS (ESI) m/z 406 MH+
Example 8 69
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 2-fluoro-benzylamide
MS (ESI) m/z 420 MH+
Example 870
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 3-fluoro-benzylamide
MS (ESI) m/z 420 MH+
Example 871
3- [ (E) -2- (4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 4-fluoro-benzylamide
MS (ESI) m/z 420 MH+
Example 872
3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 2-methoxy-benzylamide
MS (ESI) m/z 432 MH+
Example 873
3- [ (E) -2- (4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 3-methoxy-benzylamide
MS (ESI) m/z 432 MH+
Example 874
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 4-methoxy-benzylamide
MS (ESI) m/z 432 MH+
Example 87 5
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid 3,4-dimethoxy-benzylamide
MS (ESI) m/z 462 MH+
Example 87 6
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-2H-indazole-5-
carboxylic acid 2,4-dimethoxy-benzylamide
MS (ESI) m/z 462 MH+
Example 877
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 3,5-dimethoxy-benzylamide
MS (ESI) m/z 462 MH+
Example 878
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (benzo[1,3]dioxol-5-ylmethyl)-amide
MS (ESI) m/z 446 MH+
Example 87 9
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-2H-indazole-5-
carboxylic acid 3,4,5-trimethoxy-benzylamide
MS (ESI) m/z 492 MH+
Example 8 80
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 2,4,6-trimethoxy-benzylamide
MS (ESI) m/z 492 MH+
Example 881
3-[(E)-2-(4-Fluorophenyl)-vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid 2-trifluoromethyl-benzylamide
MS (ESI) m/z 470 MH+
Example 882
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 3-trifluoromethyl-benzylamide
MS (ESI) m/z 470 MH+
Example 883
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 4-trifluoromethyl-benzylamide
MS (ESI) m/z 470 MH+
Example 884
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid 3-chloro-benzylamide
MS (ESI) m/z 436 MH+
Example 885
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid 4-methanesulfonyl-benzylamide
MS (ESI) m/z 480 MH+
Example 88 6
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid (2-cyano-ethyl)-amide
MS (ESI) m/z 365 MH+
Example 887
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (4-amino-2H-pyrazol-3-ylmethyl)-amide
MS (ESI) m/z 407 MH+
Example 888
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [1-(2-hydroxyethyl)-cyclopropyl]-amide
20 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (1-benzyloxyethyl-cyclopropyl)-amide obtained by Example 814 was dissolved in 1 mL of dichloromethane, added with 6 . 4 pi of trimethylsilyliodide at 0°C under nitrogen atmosphere, and stirred at this temperature for 2 hours. The reaction solution was added with methanol to stop the reaction, and the solvent was concentrated, followed by purification by LC-MS, to afford 1. 7 mg of the title compound. MS (ESI) m/z 396 MH+ Production example 889 3-Iodo-4-methoxy-lH-indazole-5-carboxylic acid
In accordance with the method of Example 216, from 14.5 g of 3-iodo-4-methoxy-1H-indazole-5-carbonitrile obtained by Production example 321, 5. 4 g of the title compound was obtained as beige crude crystals.
1H-NMR (400 MHz, CD3OD) δ4.04 (3H, s), 7.31 (1H, d, J =8.8 Hz), 7.89 (1H, d, J = 8.8 Hz). Production example 890
3-Iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid methyl ester
3.1 g of crude 3-iodo-4-methoxy-lH-indazole-5-carboxylic acid obtained by Production example 889 was esterified in methanol in the manner as described in Production example 274, and further tritylated in the manner as described in Production example 94, to afford 2. 0 g of the title compound as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ3. 80 (3H, s), 3.94 (3H, s) , 6.22 (1H, d, J = 8.9 Hz) , 7.10-7.15 (6H, m) , 7.28-7.37 (9H, m) , 7.4 2 (1H, d, J = 8.9 Hz). Example 8 91
4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid methyl ester
550 mg of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid methyl ester obtained by Production example 890 and 160 mg of 2-vinylpyridine were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 150 mg of the title compound as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 3.87 (3H, s), 3.95 (3H, s), 7.29 (1H, dd, J = 4.8, 7.7 Hz), 7.36 (1H, d, J = 9.2 Hz), 7.60 (1H, d, J = 7.7 Hz) , 7.61 (1H, d, J = 15.9 Hz) , 7.74 (1H, d, J = 9.2 Hz), 7.81 (1H, dt, J = 1.9, 7.7 Hz), 8.08 (1H, d, J = 15.9 Hz) , 8.63 (1H, dd, J = 1.9, 4.8 Hz), 13.61 (1H, bs). Example 892 4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-lH-indazole-5-

carboxylic acid
150 mg of 4-methoxy-3-[(E)-2-(pyridin-2-yl) -vinyl] -lH-indazole-5-carboxylic acid methyl ester was treated in the similar method as described in Example 144, to afford 120 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.96 (3H, s) , 7.29 (1H, dd, J = 4.8, 7.7 Hz), 7.33 (1H, d, J = 8.6 Hz), 7.59 (1H, d, J = 7.7 Hz), 7. 61 (1H, d, J = 16.0 Hz) , 7.75 (1H, d, J=8.6Hz), 7.81 (1H, dt, J = 1.7, 7.7 Hz) , 8.09 (1H, d, J = 16.0 Hz) , 8.63 (1H, bd, J = 4.8 Hz), 12.65-12.80 (1H, bs), 13.55 (1H, bs). Example 8 93
In the similar method as described in Example 44, 4-methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid obtained by Production example 8 92 and various kinds of amine were condensed, followed by purification by LC-MS, to afford the compounds of Examples 894-898. Example 8 94
4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 335 MH+ Example 8 95
4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 375 MH+ Example 8 96 4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-2H-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 391 MH+
Example 897
4-Methoxy-3-[(E)-2-(pyridin-2-yl)-vinyl]-1H-indazole-5-
carboxylic acid (l-carbamoyl-2-phenylethyl)-amide
MS (ESI) m/z 442 MH+
Example 8 98
4-Methoxy-3- [ (E) -2- (pyridin-2-yl) -vinyl] -1H-indazole-5-
carboxylic acid (l-hydroxymethyl-2-methyl-propyl)-amide
MS (ESI) m/z 381 MH+
Example 8 99
3- [ {E) -2- ( 6-Hydroxy-pyridin-2-yl) -vinyl] -4-methoxy-1H-
indazole-5-carboxylic acid methyl ester
550 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid methyl ester obtained by Production example 890 and 270 mg of 2-tert-butoxy-5-vinylpyridine were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 120 mg of the title compound as a yellow powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.87 (3H, s), 3.94 (3H, s) , 6.50 (1H, bd, J = 9.1 Hz), 6.58 (1H, bd, J = 7.0 Hz), 7.30 (1H, d, J = 16.1 Hz), 7.35 (1H, d, J = 9.1 Hz), 7.46 (1H, dd, J = 7.0, 9.1 Hz), 7.74 (1H, d, J= 9.1 Hz), 7.83 (1H, d, J = 16.1 Hz). Example 900
3-[(E)-2-(6-Hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
120 mg of 3-[(E)-2-(6-hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid methyl ester obtained by Example 899 was treated in the similar method as described in Example 144, to afford 95 mg of the title compound as a yellow powder.
1H-NMR (400MHz, DMSO-D6) δ 3.95 (3H, s), 6.35 (1H, d, J =9.2 Hz), 6.64 (1H, bd, J = 7.1 Hz), 7.33 (1H, d, J = 16.8 Hz), 7.34 (1H, d, J= 8.8 Hz), 7.51 (1H, dd, J = 7.1, 9.2 Hz), 7.7 5 (1H, d, J = 8.8 Hz), 7.84 (1H, d, J = 16.8 Hz). Example 901
3- [ (E) -2-(6-Hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 902-906. Example 902
3-[(E)-2-(6-Hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-2H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 351 MH+ Example 903
3- [ (E) -2- (6-Hydroxy-pyridin-2-yl) -vinyl] -4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 391 MH+ Example 904
3-[(E)-2-(6-Hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (thiophene-2-ylmethyl)-amide
MS (ESI) m/z 407 MH+
Example 905
3-[(E)-2-(6-Hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (l-carbamoyl-2-phenylethyl)-amide
MS (ESI) m/z 458 MH+
Example 906
3-[(E)-2-(6-Hydroxy-pyridin-2-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (l-hydroxymethyl-2-
methyl-propyl)-amide
MS (ESI) m/z 397 MH+
Example 907
3- [ (E) -2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid methyl ester
400 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid methyl ester obtained by Production example 890 and 160 mg of 1-(5-vinylpyridin-2-yl)ethanone were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 50 mg of the title compound as an orange powder.
1H-NMR (400 MHz, DMSO-D6) δ 2.66 (3H, s), 3.87 (3H, s), 3.99 (3H, s), 7.37 (1H, d, J = 8.8 Hz), 7.69 (1H, d, J = 16.8 Hz), 7.74 (1H, d, J = 8.8 Hz), 7.84 (1H, d, J = 16.8 Hz), 7.99 (1H, d, J = 8.2 Hz), 8.32 (1H, dd, J = 2.2, 8.2 Hz), 8.98 (1H, d, J = 2.2 Hz), 13.70 (1H, bs). Example 908
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid
50 nag of 3- [ (E) -2- (6-acetylpyridin-3-yl) -vinyl] -4-methoxy-lH-indazole-5-carboxylic acid methyl ester was treated in the similar method as described in Example 144, to afford 50 mg of the title compound as an orange powder. 1H-NMR (400 MHz, DMSO-D6) δ 2.66 (3H, s), 3.99 (3H, s) , 7.33 (1H, d, J = 8.7 Hz), 7.69 (1H, d, J = 16.6 Hz), 7.55 (1H, d, J = 8.7 Hz), 7.84 (1H, d, J = 16.6 Hz), 7.99 (1H, d, J = 8.1 Hz) , 8.30 (1H, dd, J = 2.1, 8.1 Hz) , 8.98 (1H, d, J = 2.1 Hz) , 13.63 (1H, bs). Example 909
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 910-916. Example 910
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 377 MH+ Example 911
3-[(E) -2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide MS (ESI) m/z 417 MH+ Example 912
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 433 MH+
Example 913
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (l-carbamoyl-2-phenylethyl)-amide
MS (ESI) m/z 484 MH+
Example 914
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (l-hydroxymethyl-2-methyl-
propyl)-amide
MS (ESI) m/z 423 MH+
Example 915
3- [ (E) -2- (6-Acetylpyridin-3-yl) -vinyl] -4-methoxy-2H-
indazole-5-carboxylic acid (2-hydroxy-propyl)-amide
MS (ESI) m/z 395 MH+
Example 916
3-[(E)-2-(6-Acetylpyridin-3-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (2-acetylaminoethyl)-amide
MS (ESI) m/z 422 MH+
Example 917
3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid methyl ester
400 mg of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid methyl ester obtained by Production example 890 and 160 mg of 5-vinylbenzo [l,3]dioxole were reacted in the
similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 150 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 3.97 (3H, s), 4.04 (3H, s), 6.01 (2H, s), 6.83 (1H, d, J = 7.9 Hz), 7.03 (1H, dd, J = 1.6, 7.9 Hz), 7.15 (1H, d, J = 1.6 Hz), 7.21 (1H, d, J = 8.9 Hz), 7.48 (1H, d, J = 16.6 Hz), 7.58 (1H, d, J = 16.6 Hz), 7.90 (1H, d, J = 8.9 Hz). Example 918
3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
150 mg of 3-[ (E) -2-(benzo[1,3]dioxole-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acidmethyl ester was treated in the similar method as described in Example 144, to afford 130 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.94 (3H, s), 6.06 (2H, s), 6.94 (1H, d, J = 8.0 Hz), 7.09 (1H, dd, J = 1.6, 8.0 Hz) , 7.27 (1H, d, J = 8.8 Hz), 7.27 (1H, d, J=1.6Hz), 7.40 (1H, d, J=16.0 Hz), 7.4 7 (1H, d, J = 16.0 Hz), 7.72 (1H, d, J = 8.8 Hz), 12.65 (1H, bs), 13.25 (1H, bs). Example 919
3-[(E)-2-(Benzo[1, 3]dioxol-5-yl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 920-930.
Example 920
3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 378 MH+ Example 921
3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide 1H-NMR (400 MHz, CDCl3) δ 3.90 (3H, s) , 4.74 (2H, d, J = 6.0 Hz), 6.01 (2H, s), 6.34 (1H, d, J =3.9 Hz), 6.38 (1H, d, J = 3.9 Hz), 6.83 (1H, d, J = 8.0 Hz), 7.03 (1H, dd, J = 1.6, 8.0 Hz), 7.15 (1H, d, J = 1.6 Hz), 7.30 (1H, d, J = 9.0 Hz), 7.37 (1H, d, J = 16.4 Hz), 7.42 (1H, s), 7.57 (1H, d, J = 16.4 Hz), 8.16 (1H, d, J = 9.0 Hz), 8.18 (1H, bt, J = 6.0 Hz). MS (ESI) m/z 418 MH+ Example 922
3-[(E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (thiophen-2-ylmethyl)-amide MS (ESI) m/z 434 MH+ Example 923
3-[{E)-2-(Benzo[1, 3]dioxol-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (l-carbamoyl-2-phenylethyl)-amide MS (ESI) m/z 485 MH+ Example 924
3-[(E)-2-(Benzo[1, 3]dioxol-5-yl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (l-hydroxymethyl-2-methyl-propyl)-amide
MS (ESI) m/z 424 MH+
Example 925
3- [ (E) -2- (Benzo [1, 3] dioxol-5-yl) -vinyl] -4-methoxy-1H-
indazole-5-carboxylic acid (2-hydroxy-propyl)-amide
MS (ESI) m/z 396 MH+
Example 92 6
3-[ {E)-2- (Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (2-acetylaminoethyl)-amide
MS (ESI) m/z 423 MH+
Example 927
3-[(E) -2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (pyrazin-2-ylmethyl)-amide
MS (ESI) m/z 430 MH+
Example 928
3-[{E)-2-(Benzo[1, 3]dioxol-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylmethyl-amide
MS (ESI) m/z 392 MH+
Example 929
3-[{E)-2-(Benzo[1,3]dioxol-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid benzylamide
MS (ESI) m/z 428 MH+
Example 930
3-[(E)-2-(Benzo[1, 3]dioxol-5-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI) m/z 429 MH+
Example 931
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester
1.12 g of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 160 mg of 2-vinylpyrazine were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 440 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, CDCl3) δ 1.46 (3H, t, J = 7.2 Hz), 4.08 (3H, s), 4.45 (2H, q, J = 7.2 Hz), 7.26 (1H, d, J = 8.8 Hz), 7.77 (1H, d, J = 16.0 Hz), 7.90 (1H, d, J = 8.8 Hz), 8.30 (1H, d, J = 16.0 Hz), 8.45 (1H, d, J = 2.4 Hz), 8.61 (1H, dd, J = 1.6, 2.4 Hz), 8.71 (1H, d, J = 1.6 Hz). Example 932
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid
440 mg of 4-methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-1H-indazole-5-carboxylicacidethyl ester was treatedin the similar method as described in Example 144, to afford 340 mg of the title compound as a yellow powder.
1H-NMR (400 MHz, DMSO-D6) δ 3.97 (3H, s) , 7.35 (1H, d, J = 8.8 Hz), 7.68 (1H, d, J = 16.0 Hz) , 7.76 (1H, d, J= 8.8 Hz), 8.20 (1H, d, J = 16.0 Hz), 8.53 (1H, d, J = 2.4 Hz), 8.69 (1H, dd, J = 1.2, 2.4 Hz), 8.85 (1H, d, J = 1.2 Hz), 12.70-12.90 (1H, bs), 13.67 (1H, bs). Example 933
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-1H-indazole-5 -carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 934-940. Example 934
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid (pyrazin-2-ylmethyl)-amide MS (ESI) m/z 388 MH+ Example 935
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-1H-indazole-5-carboxylic acid (furan-3-ylmethyl)-amide MS (ESI) m/z 376 MH+ Example 936
4-Methoxy-3-[(E) -2-(pyrazin-2-yl)-vinyl]-lH-indazole-5-carboxylic acid cyclopropylmethyl-amide MS (ESI) m/z 350 MH+ Example 937
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-1H-indazole-5-carboxylic acid (thiophen-3-ylmethyl)-amide MS (ESI) m/z 392 MH+ Example 938
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-1H-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 336 MH+ Example 939
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid benzylamide
MS (ESI) m/z 386 MH+
Example 94 0
4-Methoxy-3-[(E)-2-(pyrazin-2-yl)-vinyl]-lH-indazole-5-
carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI) m/z 387 MH+
Example 941
3- [ {E) -2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid ethyl ester
550 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 3-fluoro-4-methoxystyrene were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 170 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 1.46 (3H, t, J = 7.2 Hz), 3.93 (3H, s), 4.04 (3H, s), 4.44 (2H, q, J = 7.2 Hz), 6.97 (1H, t, J = 8.6 Hz) , 7.21 (1H, d, J = 8.8 Hz) , 7.25-7.30 (1H, m) , 7.36 (1H, dd, J = 2.2, 12.6 Hz), 7.52 (1H, d, J = 16.4 Hz) , 7.56 (1H, d, J = 16.4 Hz), 7.90 (1H, d, J = 8.8 Hz). Example 942
3-[ (E) -2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
170 mg of 3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester was treated
in the similar method as described in Example 144, to afford 150 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.88 (3H, s), 3.96 (3H, s), 7.20 (1H, t, J = 8.8 Hz), 7.29 (1H, d, J = 8.8 Hz), 7.42 (1H, bd, J = 8.8 Hz), 7.46 (1H, d, J = 16.1 Hz), 7.50 (1H, d, J = 16.1 Hz) , 7.55 (1H, dd, J = 2.2, 12.8 Hz) , 7.74 (1H, d, J = 8.8 Hz) , 12.60 (1H, bs), 13.42 (1H, s) . Example 94 3
3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-l H-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 944-953. Example 94 4
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (pyrazin-2-ylmethyl) amide MS (ESI) m/z 434 MH+ Example 94 5
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (furan-2-ylmethyl) amide MS (ESI) m/z 422 MH+ Example 94 6
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylmethyl amide MS (ESI) m/z 396 MH+ Example 947
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-2-ylmethyl) amide
MS (ESI) m/z 438 MH+
Example 948
3-[(E)-2~(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 382 MH+
Example 94 9
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid benzylamide
MS (ESI) m/z 432 MH+
Example 950
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (pyridin-3-ylmethyl) amide
MS (ESI) m/z 433 MH+
Example 951
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (pyridin-2-ylmethyl) amide
MS (ESI) m/z 433 MH+
Example 952
3-[[E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-3-ylmethyl) amide
MS (ESI) m/z 438 MH+
Example 953
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 423 MH+
Example 954
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid ethyl ester
550 mg of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 4-fluoro-3-methoxystyrene were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 160 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 1.45 (3H, t, J = 7.2 Hz), 3.94 (3H, s), 4.06 (3H, s), 4.4 5 (2H, q, J = 7.2 Hz), 7.06-7.17 (2H, m), 7.19 (1H, dd, J = 1.6, 8.0 Hz), 7.23 (1H, d, J = 8.8 Hz), 7.57 (1H, d, J = 16.4 Hz), 7.61 (1H, d, J = 16.4 Hz), 7.91 (1H, d, J = 8.8 Hz). Example 955
3-[(E) -2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
160 mg of 3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester was treated in the similar method as described in Example 144, to afford 140 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.93 (3H, s) , 3.96 (3H, s), 7.22-7.28 (2H, m), 7.30 (1H, d, J = 8.4 Hz), 7.42 (1H, dd, J = 1.6, 7.6 Hz) , 7.54 (1H, d, J = 16.3 Hz) , 7.55 (1H, d, J = 16.3 Hz) , 7.74 (1H, d, J = 8.4 Hz), 12.60-12.80 (1H, bs), 13.46 (1H, bs).
Example 956
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 957-966. Example 957
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (pyrazin-2-ylmethyl) amide MS (ESI) m/z 434 MH+ Example 958
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (furan-2-ylmethyl) amide MS (ESI) m/z 422 MH+ Example 959
3- [ (E) -2- (4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylmethyl amide MS (ESI) m/z 396 MH+ Example 960
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (thiophen-2-ylmethyl) amide MS (ESI) m/z 438 MH+ Example 961
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 382 MH+
Example 962
3-[ (E) -2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid benzylamide
MS (ESI) m/z 432 MH+
Example 963
3- [ (E) -2- (4-Fluoro-3-methoxyphenyl) -vinyl] -4-metboxy-lH-
indazole-5-carboxylic acid (pyridin-3-ylmethyl) amide
MS (ESI) m/z 433 MH+
Example 964
3- [ (E) -2- (4-Fluoro-3-methoxyphenyl) -vinyl] -4-methoxy-1H~
indazole-5-carboxylic acid (pyridin-2-ylmethyl) amide
MS (ESI) m/z 433 MH+
Example 965
3-[ (E) -2- (4-Fluoro-3-methoxyphenyl) -vinyl] -4-methoxy-1H-
indazole-5-carboxylic acid (thiophen-3-ylmethyl) amide
MS (ESI) m/z 438 MH+
Example 966
3- [ (E) -2- (4-Fluoro-3-methoxyphenyl) -vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 423 MH+
Example 967
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid ethyl ester
410 mg of 3-iodo-4-methoxy~l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 3, 4-dif luorostyrene were reacted in the similar
method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 110 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 1.45 (3H, t, J = 7.2 Hz), 4.05 (3H, s), 4.46 (2H, q, J = 7.2 Hz), 7.17 (1H, dt, J = 8.1, 9.8 Hz), 7.23 (1H, d, J = 9.0 Hz), 7.26-7.31 (1H, m), 7.40 (1H, ddd, J = 2.1, 8.1, 11.4 Hz), 7.56 (1H, d, J = 16.4 Hz), 7.58 (1H, d, J = 16.4 Hz), 7.92 (1H, d, J = 9.0 Hz). Example 968
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
110 mg of 3-[(E)-2-(3,4-difluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid ethyl ester was treated in the similar method as described in Example 144, to afford 100 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.97 (3H, s) , 7.31 (1H, d, J = 8.8 Hz), 7.46 (1H, dt, J = 8.4, 10.4 Hz), 7.49-7.55 (1H, m), 7.54 (1H, d, J = 16.4 Hz), 7.57 (1H, d, J = 16.4 Hz), 7.75 (1H, d, J = 8.8 Hz), 7.77 (1H, ddd, J = 1.9, 8.4, 12.1 Hz), 12.75 (1H, bs) , 13.51 (1H, bs) . Example 969
3-[(E)-2-(3, 4-Difluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 970-979.
Example 970
3-[(E) -2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (pyrazin-2-ylmethyl) amide
MS (ESI) m/z 422 MH4
Example 971
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (furan-2-ylmethyl) amide
MS (ESI) m/z 410 MH+
Example 972
3-[(E)-2-(3, 4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylmethyl amide
MS (ESI) m/z 384 MH+
Example 973
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-2-ylmethyl) amide
MS (ESI) m/z 426 MH+
Example 974
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 370 MH+
Example 975
3-[(E)-2-(3, 4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid benzylamide
MS (ESI) m/z 420 MH+
Example 97 6
3-[(E) -2-(3, 4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (pyridin-3-ylmethyl) amide
MS (ESI) m/z 421 MH+
Example 977
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (pyridin-2-ylmethyl) amide
MS (ESI) m/z 421 MH+
Example 978
3- [ (E) -2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-3-ylmethyl) amide
MS (ESI) m/z 426 MH+
Example 97 9
3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 411 MH+
Example 980
3- [ (E) -2- (2, 4-Dif luorophenyl) -vinyl] -4-methoxy-lH-
indazole-5-carboxylic acid ethyl ester
410 mg of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 2, 4-difluorostyrene were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 50 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 1.46 (3H, t, J = 7.2 Hz), 4.05 (3H, s), 4.43 (2H, q, J=7.2Hz), 6.87 (1H, ddd, J = 2.6, 8.4, 11.2 Hz), 6.93 (1H, dt, J = 2.7, 8.4 Hz), 7.24 (1H, d, J = 8.8 Hz) ,
7.62 (1H, dt, J = 6.4, 8.4 Hz) , 7.70 (1H, d, J = 16.4 Hz), 7.75 (1H, d, J = 16.4 Hz), 7.92 (1H, d, J = 8.8 Hz). Example 981
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid
50 mg of 3-[ (E) -2-(2,4-difluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester was treated in the similar method as described in Example 144, to afford 40 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.95 (3H, s) , 7.17 (1H, dt, J = 2.4, 8.8 Hz) , 7.30-7.38 (1H, m) , 7.32 (1H, d, J = 8.8 Hz) , 7.61 (1H, d, J = 16.4 Hz), 7.67 (1H, d, J = 16.4 Hz), 7.75 (1H, d, J = 8.8 Hz), 7.88 (1H, dt, J= 6.8, 8.8 Hz), 12.68-12.82 (1H, bs), 13.52 (1H, bs) . Example 982
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid and various kinds of amine were condensed in the similar method as described in Example 44, followed by purification by LC-MS, to afford the compounds of Examples 983-992. Example 98 3
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid (pyrazin-2-ylmethyl) amide MS (ESI) m/z 422 MH+ Example 984 3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-lH-

indazole-5-carboxylic acid (furan-2-ylmethyl) amide
MS (ESI) m/z 410 MH+
Example 985
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylmethyl amide
MS (ESI) m/z 384 MH+
Example 98 6
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-2-ylmethyl) amide
MS (ESI) m/z 426 MH+
Example 987
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 370 MH+
Example 988
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid benzylamide
MS (ESI) m/z 420 MH+
Example 98 9
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (pyridin-3-ylmethyl) amide
MS (ESI) m/z 421 MH+
Example 990
3- [ (E) -2- (2, 4-Difluorophenyl) -vinyl] -4-methoxy-1H-indazole-
5-carboxylic acid (pyridin-2-ylmethyl) amide
MS (ESI) m/z 421 MH+
Example 991
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-lH-indazole-
5-carboxylic acid (thiophen-3-ylmethyl) amide
MS (ESI) m/z 426 MH+
Example 992
3-[(E)-2-(2,4-Difluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 411 MH+
Production example 993
6-Fluoro-3-[(E) -2-(4-fluoro-3-methoxyphenyl)-vinyl]-5-
nitro-1-trityl-lH-indazole
505 mg of 6-fluoro-3-iodo-5-nitro-l-trityl-lH-indazole obtained by Production example 747 and 304 mg of 3-methoxy-4-fluorostyrene were reacted in the similar method as described in Production example 181, to afford 145 mg of the title compound as a yellow powder.
1H-NMR (400 MHz, DMSO-D6) δ 3.93 (3H, s) , 6.16 (1H, d, J =12.4 Hz), 7.21-7.30 (6H, m), 7.30-7.45 (11H, m), 7.46 (1H, d, J = 16.4 Hz), 7.57 (1H, d, J = 2.0, 8.4 Hz), 7.62 (1H, d, J = 16.4 Hz), 9.12 (1H, d, J = 7.6 Hz). Production example 994
6-Fluoro-3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine
145 mg of 6-fluoro-3-[ (E) -2-(4-fluoro-3-methoxyphenyl) -vinyl] -5-nitro-l-trityl-1H-indazole obtained by Production example 993 was reduced in the similar method as
described in Production example 182, to afford 130 mg of the title compound as a pale brown oil.
1H-NMR (400 MHz, CDCl3) δ 3.93 (3H, s), 6.16 (1H, d, J = 11.9 Hz) , 7.00-7.2 6 (2H, m) , 7.14 (1H, dd, J = 2.0, 8.1 Hz) , 7.20-7.35
(18H, m) . Example 995
In the similar method as described in Example 183, from 6-f luoro-3- [ (E) -2- (4-f luoro-3-methoxyphenyl) -vinyl] -1-trityl-lH-indazol-5-ylamine and various kinds of carboxylic acid, the compounds of Examples 996-1004 were obtained. Example 996
Cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide MS (ESI) m/z 370 MH+ Example 997 1-Hydroxycyclopropane carboxylic acid {6-fluoro-3-[(E)-2-
(4-fluoro-3-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide MS (ESI) m/z 386 MH+ Example 998
1-Acetyl piperidine-4-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-1H-indazol-5-yl}amide MS (ESI) m/z 455 MH+ Example 999
Tetrahydrofuran-3-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide MS (ESI) m/z 400 MH+
Example 1000
1-Methylaminocyclopropane carboxylic acid { 6-fluoro-3- [ (E) -2-
(4-fluoro-3-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide
MS (ESI) m/z 399 MH+
Example 1001
N-{6-Fluoro-3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-1H-
indazol-5-yl}-2-(thiophen-2-yl)acetamide
MS (ESI) m/z 426 MH+
Example 1002
Furan-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluoro-3-methoxyphenyl) -vinyl ] -1H-indazol-5-yl} amide
MS (ESI) m/z 396 MH+
Example 1003
2-Cyclopropyl-N-{6-fluoro-3-[(E)-2-(4-fluoro-3-
methoxyphenyl)-vinyl]-lH-indazol-5-yl}acetamide
MS (ESI) m/z 384 MH+
Example 1004
(2S,4R)-4-hydroxypyrrolidine-2-carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluoro-3-methoxyphenyl)-vinyl]-1H~
indazol-5-yl}amide
MS (ESI) m/z 415 MH+
Production example 1005
6-Fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-5-
nitro-1-trityl-2H-indazole
505 mg of 6-fluoro-3-iodo-5-nitro-l-trityl-lH-indazole
obtained by Production example 747 and 304 mg of
4-methoxy-3-fluorostyrene were reacted in the similar method
as described in Production example 181, to afford 160 mg of the
title compound as a yellow powder.
1H-NMR (400 MHz, DMSO-D6) δ 3.87 (3H, s) , 6.11 (1H, d, J =12.4
Hz) , 7.18 (1H, t, J = 8.8Hz), 7.18-7.27 (6H, m), 7.31-7.44 (11H,
m), 7.60 (1H, d, J = 16.4 Hz) , 7.75 (1H, d, J = 2.0, 13.2 Hz) ,
9.15 (1H, d, J = 7.6 Hz).
Production example 1006
6-Fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-1-
trityl-1H-indazol-5-ylamine
160 mg of 6-fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-5-nitro-l-trityl-lH-indazole obtained by Production example 1005 was reduced in the similar method as described in Production example 182, to afford 140 mg of the title compound as a pale brown oil.
1H-NMR (400 MHz, CDCl3) δ 3.89 (3H, s), 6.15 (1H, d, J = 12.6 Hz), 6.92 (1H, t, J = 8.8 Hz), 7.15-7.38 (20H, m). Example 1007
In the similar method as described in Example 183, from 6-fluoro-3-t(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine and various kinds of carboxylic acid, the compounds of Examples 1008-1016 were obtained. Example 1008
Cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-1H-indazol-5-yl}amide
MS (ESI) m/z 370 MH+ Example 1009
1-Hydroxycyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-1H-indazol-5-yl}amide MS (ESI) m/z 386 MH+ Example 1010
1-Acetyl piperidine-4-carboxylic acid {6-fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide MS (ESI) m/z 455 MH+ Example 1011
Tetrahydrofuran-3-carboxylic acid
{ 6-f luoro-3- [ (E) -2- (3-f luoro-4-methoxyphenyl) -vinyl] -1H-indazol-5-yl}amide MS (ESI) m/z 400 MH+ Example 1012
1-Methylaminocyclopropane carboxylic acid { 6-f luoro-3- [ (E) -2-(3-fluoro-4-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide MS (ESI) m/z 399 MH+ Example 1013
N-{6-Fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-1H-indazol-5-yl}-2-(thiophen-2-yl)acetamide MS (ESI) m/z 426 MH+ Example 1014
Furan-2-carboxylic acid {6-fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]-lH-indazol-5-yl}amide
MS (ESI) m/z 396 MH+
Example 1015
2-Cyclopropyl-N-{6-fluoro-3-[(E)-2-(3-fluoro-4-
methoxyphenyl)-vinyl]-lH-indazol-5-yl}acetamide
MS (ESI) m/z 384 MH+
Example 1016
(25, 4R) -4-Hydroxypyrrolidine-2-carboxylic acid
{6-fluoro-3-[(E)-2-(3-fluoro-4-methoxyphenyl)-vinyl]—1H—
indazol-5-yl}amide
MS (ESI) m/z 415 MH+
Example 1017
96 pieces of polystyrene labeled with TRANSTEM™ (SynPhase Polystyrene D-Seriese,Trityl™) were prepared and left in 130 mL of a solution of 20 mL acetyl chloride in dichloromethane for 3 hours. After removing the solution, the resin pieces were washed three times with dichloromethane, dried under reduced pressure, and the resin was heated at 80°C for 4 hours in 100 mL of solution of 4.7 g of
3-iodo-4-methoxy-lH-indazole-5-carboxylic acid methyl ester produced as intermediate of Production example 890 and 2.2 mL of diisopropylethylamine in N-methylpyrrolidone. After removing the solution, the resin was washed successively with iV-methylpyrrolidone, ethanol, water, methanol and tetrahydrofuran, and dried under reduced pressure.
The resultant resin pieces were divided into several groups each consisting of 10 pieces in accordance with the label, and
each resin piece was added to 10mL of previously-prepared several kinds of 0.5 M styrene compound in a mixed solution of N-methylpyrrolidone-triethylamine (7.5:2.5). To each reaction solution were added 150 mg of 0.5M 2-(di-tert-butylphosphino)biphenyl and 110 mg of palladium acetate (II), and heated at 100°C for 8 hours. After removing the solution, the resin was washed in the manner as described in the above operation, and dried under reduced pressure.
The resultant resin was heated at reflux for 12 hours in a mixed solution of ethanol (2 mL), dioxane(0.7 mL), 5N sodium hydroxide aqueous solution (2 mL) , washed in the similar method as described in the above operation, and dried under reduced pressure.
Then the resin pieces were divided into several groups in accordance with the label, and each resin piece was added with 15 mL of a previously-prepared different 0.5 M amine in N-methylpyrrolidone. To each container, 1.15 g of 1-hydroxybenzotriazole monohydrate, 1.2 mL of l-ethyl-3-(3-dimethylaminopropyl)carbodiimide(=WSC) and 2.0 mL of diisopropylethylamine were successively added, sonicated for 1 hour, and then left overnight at room temperature. After removing the solution, the resin was washed in the manner as described in the above operation, and dried under reduced pressure. In accordance with the label of resin, they were arranged in a 96-well pin plate.
The resin was immersed in a mixed solution of 0.5 mL
trifluoroacetic acid/0.1 mL triisopropylsilane/O.5 mL
dichloromethane prepared in advance in 96-well plate, and after
conducting sonication for 10 minutes, they were left for 30
minutes. This operation was repeated twice, and then the resin
was washed with 1 mL of N,N-dimethylformamide. Next, nitrogen
was blown in to the acid-treated wells, and the resultant residues
were dissolved in N,N-dimethylformamide solution obtained
during washing operation, followed by purification and
separation by LC-MS, to afford the compounds of Examples
1018-1029.
Example 1018
4-Methoxy-3-[(E)-2-(4-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid (pyrazin-2-ylmethyl)-amide
MS (ESI) m/z 416 MH+
Example 1019
4-Methoxy-3-[(E)-2-(4-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 404 MH+
Example 1020
4-Methoxy-3-[{E)-2-(4-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 420 MH+
Example 1021
4-Methoxy-3-[(E)-2-(4-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid benzylamide
MS (ESI) m/z 414 MH+
Example 1022
4-Methoxy-3-[(E)-2-(4-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI) m/z 415 MH+
Example 1023
3-[{E)-2-(3,4-Dimethoxy-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (pyrazin-2-ylmethyl)-amide
MS (ESI) m/z 446 MH+
Example 1024
3-[(E) -2-(3,4-Dimethoxy-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (furan-3-ylmethyl)-amide
MS (ESI) m/z 434 MH+
Example 1025
3-[(E)-2-(3,4-Dimethoxy-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylmethyl-amide
MS (ESI) m/z 408 MH+
Example 1026
3-[(E)-2-(3,4-Dimethoxy-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-2-ylmethyl)-amide
MS (ESI) m/z 450 MH+
Example 1027
3-[(E)-2-(3,4-Dimethoxy-phenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 394 MH+
Example 1028
3-[(E)-2-(3,4-Dimethoxy-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid benzylamide
MS (ESI) m/z 444 MH+
Example 1029
3- [ (E) -2- (3, 4-Dimethoxy-phenyl) -vinyl] -4-methoxy-1H-
indazole-5-carboxylic acid (pyridin-3-ylmethyl)-amide
MS (ESI) m/z 445 MH+
Example 1030
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-1H-indazol-5-ylamine obtained by Production example 182 and various kinds of carboxylic acid were treated in the similar method as described in Example 183, to afford the compounds of Examples 1031-1053. Example 1031
3-Oxo-cyclopentane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 382 MH+ Example 1032
1-(4-Methoxy-phenyl)-cyclopentane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide
MS (ESI) m/z 474 MH+ Example 1033
2,2-Dimethyl-5-oxo-tetrahydrofuran-3-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 412 MH+
Example 1034
(45)-2-Oxo-thiazolidine-4-carboxylic acid
{ 6-f luoro-3- [ (E) -2- (4-f luorophenyl) -vinyl] -1H-indazol-5-
yl}-amide
MS (ESI) m/z 401 MH+
Example 1035
5-Oxo-tetrahydrofuran-2-carboxylic acid
{ 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 384 MH+
Example 1036
(2S) -Pyrrolidine-2-carboxylic acid {6-fluoro-3-[ (E) -2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 369 MH+
Example 1037
(2R)-Pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 369 MH+
Example 1038
(4S)-Thiazolidine-4-carboxylic acid {6-fluoro-3-[ (E) -2- (4-
f luorophenyl) -vinyl ] -2H-indazol-5-yl) -amide
MS (ESI) m/z 387 MH+
Example 1039
l-7Amino-cyclopentane carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-amide
MS (ESI) m/z 383 MH+ Example 1040
Tetrahydrofuran-2-carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 370 MH+ Example 1041
Tetrahydrofuran-3-carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 370 MH+ Example 1042
(2R)-2-Rmino-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 411 MH+ Example 1043
5-Oxo-l-(thiophen-2-ylmethyl)-pyrrolidine-3-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 479 MH+ Example 1044
1-(Furan-2-ylmethyl)-5-oxo-pyrrolidine-3-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-yl }-amide MS (ESI) m/z 463 MH+

Example 1045
Piperidine-3-carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 383 MH+
Example 104 6
Piperidine-4-carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-amide
MS (ESI) m/z 383 MH+
Example 1047
Piperidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 383 MH+
Example 104 8
l-Acetyl-piperidine-4-carboxylic acid
{6-fluoro-3- [ (E) -2- (4-f luorophenyl) -vinyl] -2H-indazol-5-
yl}-amide
MS (ESI) m/z 425 MH+
Example 104 9
1-Amino-cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 355 MH+
Example 1050
2-Oxo-imidazolidine-4-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 384 MH+ Example 1051
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-2-(morpholin-4-yl)-acetamide MS (ESI) m/z 399 MH+ Example 1052
(2S)-l-Methyl-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 383 MH+ Example 1053
2-Amino-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-2-(pyridin-3-yl)-acetamide MS (ESI) m/z 406 MH+ Example 1054
l-Ethyl-3-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H~ indazol-5-yl}-urea
10 mg of 6-fluoro-3-[2-{4-f luorophenyl) -vinyl] -trityl-1H-indazol-5-ylamine obtained by Production example 182 and 3 µl of ethyl isocyanate was dissolved in 5 mL of chloroform, and heated at reflux for 2 hours. The reaction solution was allowed to cool to room temperature, added with 0 . 5 mL of trif luoroacetic acid, stirred at room temperature for 30 minutes, and the reaction mixture was purified and separated by LC-MS, to afford 0.67 mg of the title compound as pale yellow powder.
MS (ESI) m/z 343 MH+ Example 1055
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine obtainedby Production example 182 and various kinds of isocyanate were treated in the similar method as described in Example 1054, to afford the compounds of Examples 1056-1060. Example 1056
l-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-3-propyl-urea MS (ESI) m/z 357 MH+ Example 1057
l-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-yl}-3-isopropyl-urea MS (ESI) m/z 357 MH+ Example 1058
l-tert-Butyl-3-{6-fluoro-3-[ (E)-2-(4-fluorophenyl)-vinyl]-ltf-indazol-5-yl}-urea MS (ESI) m/z 371 MH+ Example 1059
l-Cyclohexyl-3-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-urea MS (ESI) m/z 397 MH+ Example 1060
l-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-3-phenyl-urea
MS (ESI) m/z 391 MH+ Example 1061
3-Vinyl-4-methoxy-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 323 and various kinds of aryl halide were subjected to Heck reaction in accordance with Production example 324, and deprotected in accordance with Example 16, followed by separation and purification by LC-MS, to afford the compounds of Examples 1062-1065. Example 1062
4-Methoxy-3-[(E)-2-(4-trifluoromethyl-phenyl)-vinyl]-1H-ind azole-5-carbonitrile MS (ESI) m/z 344 MH+ Example 1063
4-Methoxy-3-[(E)-2- (3-trifluoromethyl-phenyl)-vinyl]-lH-ind azole-5-carbonitrile MS (ESI) m/z 344 MH+ Example 1064
3-[(E)-2-(5-Hydroxymethyl-pyridin-3-yl)-vinyl]-4-methoxy-lH -indazole-5-carbonitrile MS (ESI) m/z 307 MH+ Example 10 65
4-Methoxy-3-[(E)-2-(2-trifluoromethyl-phenyl)-vinyl]-1H-ind azole-5-carbonitrile MS (ESI) m/z 344 MH+ Production example 1066 4-Methoxy-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid
ethyl ester
In accordance with Production example 123, from 2.80 g of 3-iodo-4-methoxy-l-trityl-1H-indazole-5-carboxylic acid ethyl ester obtained by Production example 276, 1.87 g of the title compound was obtained as ocher crystals. 1H-NMR (4 00 MHz, CDCl3 ) δ 1.37 ( 3H, t, J = 6.8 Hz ) , 4.00 ( 3H, s ), 4.35 ( 3H, q, J = 6.8 Hz ), 5.36 ( 1H, d, J = 16.0 Hz ),
6.07 - 6.14 ( 2H, m ), 7.18 - 7.23 ( 17H, m )
Production example 1067
4-Methoxy-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid
In accordance with Production example 350, from 1.87 g of 4-methoxy-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid ethyl ester, 1.07 g of the title compound was obtained as white crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.89 ( 3H, s ) , 5.37 ( 1H, d, J = 11.2 Hz ) , 5.91 ( 1H, d, J = 17.6 Hz ) , 6.07 ( 1H, d, 8 . 8 Hz ) ,
7.08 - 7.15 ( 7H, m ), 7.26 - 7.35 (10H, m )
Production example 1068
4-Methoxy-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid cyclopropylamide
In accordance with Production example 127, from 400 mg of 4-methoxy-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid and cyclopropylamine, 4 64 mg of the title compound was obtained as white crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.48 - 0.52 ( 2H, m ) , 0.63 - 0.66 ( 2H, m ), 2.79 - 2.82 ( 1H, m ), 3.84 ( 3H, s ), 5.37 ( 1H,
d, J = 11.2 Hz ), 5.92 ( 1H, d, J = 17.6 Hz ), 6.07 ( 1H, d, J = 8.8 Hz ) , 7.04 ( 1H, d, J = 8.8 Hz ) , 7.08-7.16 ( 6H, m ) , 7.28 - 7.36 ( 10H, m ), 8.26 ( 1H, d, J = 4.8 Hz ) Production example 1069
4-Methoxy-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid [(1S) -(2-hydroxy-l-phenyl-ethyl)]-amide
In accordance with Production example 127, from 310 mg of 4-methoxy-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid obtained by Production example 1067 and
(2S) -2-amino-2-phenyl-ethanol, 270 mg of the title compound was obtained as white crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.60 - 3.63 ( 2H, m ) , 3.81 ( 3H, s ), 4.92 ( 1H, t, J = 6.8 Hz ), 5.10 ( 1H, br s ), 5,37 ( 1H, d, J = 11.2 Hz ), 5.92 ( 1H, d, J = 17.6 Hz ), 6.08 ( 1H, d, J = 8.8 Hz ), 7.08 - 7.36 ( 22H, m ) , 8.65 ( 1H, d, J = 8. 4 Hz ) Production example 1070
4-Methoxy-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
In accordance with Production example 127, from 542 mg of 4-methoxy-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid obtained by Production example 1067 and furan-2-ylmethylamine, 329 mg of the title compound was obtained as white crystals. 1H-NMR ( 400 MHz, CDC13 ) δ 3.84 ( 3H, s ), 4.65 ( 2H, d, J = 5.2 Hz ), 5.37 ( 1H, d, J = 11.2 Hz ), 6.07 ( 1H, d, 17.6 Hz ), 6.18 ( 1H, d, J = 8.8 Hz ), 6.30 ( 1H, d, J = 16.8 Hz ), 7.06 - 7.27 ( 18H, m ), 7.63 ( 1H, d, J = 9.6 Hz ), 8.05 ( 1H, br
s )
Production example 1071
3-Bromo-7-fluoro-1H-indazole-5-carboxylic acid
In accordance with Production example 7, from 10.00 g of 3-bromo-7-fluoro-l-trityl-lH-indazole-5-carbonitrile obtained by Production example 122, 3.80 g of the title compound was obtained as ocher crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 7.25-7.39 ( 1H, br s ) , 7.75 ( 1H, d, J = 11.6 Hz ), 8.03 ( 1H, s ) Production example 1072 7-Fluoro-l-trityl-3-vinyl-lH-indazole-5-carboxylic acid
From 3.80 g of 3-bromo-7-fluoro-lH-indazole-5-carboxylic acid, an ester compound was obtained in accordance with Production example 274, then the ester was tritylated in accordance with Production example 22, and a vinyl group was introduced in accordance with Production example 123 . Further, by hydrolyzing the ester in accordance with Production example 350, 410 mg of the title compound was obtained as white crystals. 1H-NMR ( 400 MHz, CDC13 ) δ 5.53 ( 1H, d, J = 11.2 Hz ), 6.19 ( 1H, d, J = 17.6 Hz ), 7.10 - 7.38 ( 16H, m ), 7.45 ( 1H, d, J = 11.2 Hz ), 8.53 ( 1H, s ) Production example 1073
7-Fluoro-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid cyclopropylamide
In accordance with Production example 127, from 120 mg of 7-fluoro-l-trityl-3-vinyl-1H-indazole-5-carboxylic acid and
cyclopropylamine, 73 mg of the title compound was obtained as white crystals.
1H-NMR ( 400 MHz, CDC13 ) δ 0.58 - 0.62 ( 2H, m ), 0.84 - 0.91 ( 2H, m ), 2.81 - 2.94 ( 1H, m ) , 5.51 ( 1H, d, J = 11.2 Hz ), 6.04 ( 1H, d, J = 18.0 Hz ), 6.21 ( 1H, br s ) , 6.93 ( 1H, dd, J = 18.0, 18.0 Hz ), 7.10 - 7.16 ( 6H, m ), 7.22 - 7.32 ( 10H, m ) , 8.09 ( 1H, s ) Example 1074
Vinyl compounds obtained by Production examples 1068-1070 and 1073 and various kinds of aryl halide were subjected to Heck reaction in accordance with Production example 324, and deprotectedinaccordancewithExample 16, followedby separation and purification by LC-MS, to afford the compounds of Examples 1075-1125. Example 1075
3-[(E)-2-(2-Hydroxymethyl-phenyl)-vinyl]-4-methoxy-lH-indaz ole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 364 MH+ Example 107 6
3- [ (E) -2-(3-Hydroxymethyl-phenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid cyclopropylamide MS (ESI) m/z 364 MH+ Example 1077
7-Fluoro-3-[(E)-2-(4-trifluoromethyl-phenyl)-vinyl]-1H-indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-phenyl-ethyl)]-amide
MS (ESI) m/z 470 MH+
Example 1078
4-Methoxy-3-[(E)-2-(2-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 364 MH+
Example 107 9
4-Methoxy-3-[(E)-2-(3-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 364 MH+
Example 1080
4-Methoxy-3-[(E)-2-(4-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 364 MH+
Example 1081
3-[(E) -2-(4-Hydroxymethyl-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 364 MH+
Example 1082
3-{(E)-2-[4-(2-Hydroxyethyl)-phenyl]-vinyl}-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 378 MH+
Example 1083
3- [ (E) -2-(4-Cyanomethyl-phenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 373 MH+
Example 1084
3-[(E)-2-(4-Acetylaminophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid [ (1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 471 MH+
Example 1085
3-[(E)-2-(4-Dimethylamino-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 457 MH+
Example 1086
3-[(E)-2-(3-Dimethylamino-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 457 MH+
Example 1087
4-Methoxy-3-[(E)-2-(2-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid [(1S)-(2-hydroxy-l-phenyl~ethyl)]-amide
MS (ESI) m/z 444 MH+
Example 1088
4-Methoxy-3-[(E)-2-(3-methoxy-phenyl)-vinyl]-lH-indazole-5-
carboxylic acid [(1S)-(2-hydroxy-l-phenyl-ethyl)]-amide
MS (ESI) m/z 444 MH+
Example 1089
4-Methoxy-3- [ (E) -2- (4-methoxy-phenyl) -vinyl] -17i-indazole-5-
carboxylic acid [(1S)-(2-hydroxy-l-phenyl-ethyl)]-amide
MS (ESI) m/z 444 MH+
Example 1090
3-[(E)-2-(3-Hydroxymethyl-phenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 444 MH+
Example 1091
4-Methoxy-3-[(E)-2-(3-methoxymethyl-phenyl)-vinyl]-1H-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 458 MH+
Example 10 92
3- [ (E) -2- (3-Aminomethyl-phenyl) -vinyl] -4-methoxy-lH-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 443 MH+
Example 1093
3-{(E)-2-[3-(Acetylamino-methyl)-phenyl]-vinyl}-4-
methoxy-lH-indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 485 MH+
Example 1094
3- [ (E) -2- (4-Hydroxymethyl-phenyl) -vinyl] -4-methoxy-1H-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 444 MH+
Example 1095
4-Methoxy-3-[(E)-2-(4-methoxymethyl-phenyl)-vinyl]-1H-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 458 MH+
Example 1096
3-[(E)-2-(4-Aminomethyl-phenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 443 MH+
Example 1097
3-{(E)-2-[4-(Acetylamino-methyl)-phenyl]-vinyl}-4-
methoxy-lH-indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 485 MH+
Example 1098
4-Methoxy-3-[(E) -2-(thiophen-3-yl)-vinyl]-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 340 MH+
Example 1099
4-Methoxy-3-[(E) -2-(thiophen-3-yl)-vinyl]-lH-indazole-5-
carboxylic acid [(1S)-(2-hydroxy-l-phenyl-ethyl)]-amide
MS (ESI) m/z 420 MH+
Example 1100
4-Methoxy-3-[(E) -2-(thiophen-3-yl)-vinyl]-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 380 MH+
Example 1101
4-Methoxy-3-[(E)-2-(3-methyl-thiophen-2-yl)-vinyl]-1H-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 354 MH+
Example 1102
4-Methoxy-3-[(E)-2-(3-methyl-thiophen-2-yl)-vinyl]-1H-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 434 MH+
Example 1103
4-Methoxy-3-[(E)-2-(3-methyl-thiophen-2-yl)-vinyl]-1H-
indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 394 MH+
Example 1104
3- [ (E) -2-(5-Acetyl-thiophen-2-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 382 MH+
Example 1105
3-[(E)-2-(5-Acetyl-thiophen-2-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid [(1S)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 462 MH+
Example 1106
3-[(E)-2-(5-Acetyl-thiophen-2-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 422 MH+
Example 1107
3-[ (E) -2-(Furan-3-yl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 324 MH+
Example 1108
3-[(E)-2-(Furan-3-yl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [(15)-(2-hydroxy-l-phenyl-ethyl)]-amide
MS (ESI) m/z 404 MH+
Example 1109
3-[ (E) -2-(Furan-3-yl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 364 MH+
Example 1110
4-Methoxy-3-[(E)-2-(thiazol-2-yl)-vinyl]-1H-indazole-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 341 MH+
Example 1111
4-Methoxy-3-[(E)-2-(thiazol-2-yl)-vinyl]-1H-indazole-5-
carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 381 MH+
Example 1112
3-[(E)-2-(6-Fluoro-pyridin-3-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 353 MH+
Example 1113
3-[(E) -2-(6-Fluoro~pyridin-3-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid [(15)-(2-hydroxy-l-
phenyl-ethyl)]-amide
MS (ESI) m/z 433 MH+
Example 1114
3-[(E)-2-(6-Fluoro-pyridin-3-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (furan-2-ylmethyl)-amide
MS (ESI) m/z 393 MH+
Example 1115
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-2-(2-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 340 MH+
Example 1116
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-2-(3-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 340 MH+
Example 1117
Cyclopropane carboxylic acid
{7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-yl
}-amide
MS (ESI) m/z 340 MH+
Example 1118
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-styryl]-1H-
indazol-5-yl}-amide
MS (ESI) m/z 322 MH+
Example 1119
Cyclopropane carboxylic acid { 7-fluoro-3-[(E) -2-(thiophen-2-
yl)-vinyl]-1H-indazol-5-yl}-amide
MS (ESI) m/z 328 MH+
Example 1120
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-2-(thiophen-3-
yl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 328 MH+
Example 1121
Cyclopropane carboxylic acid {7-fluoro-3-[(E) -2-(pyridin-2-
yl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 323 MH+
Example 1122
Cyclopropane carboxylic acid {7-fluoro-3-[(E) -2-(pyridin-3-
yl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 323 MH+
Example 1123
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-2-(pyridin-4-
yl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 323 MH+
Example 1124
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-2-(6-
methoxy-pyridin-2-yl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 353 MH+
Example 1125
Cyclopropane carboxylic acid {7-fluoro-3-[(E)-2-(6-
methoxy-pyridin-3-yl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 353 MH+
Production example 1126
3-Iodo-l-trityl-lH-pyrazolo[3,4-c]pyridine-5-carboxylie
acid
To a suspension of 3.0 g of 3-iodo-l-trityl-lH-pyrazolo[3,4-c]pyridine-5-carbonitrile obtained by Production example 431 in 20 mL of ethanol was added 20mL of 4N lithium hydroxide aqueous solution at room temperature, and heated at reflux for a day. After cooling to room temperature, the solution was neutralized with 5N hydrochloric acid aqueous solution, and the precipitated crystals were collected by filtration. The crystals were washed successively with water and diethyl ether, to afford 3.0 g of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 7 .14-7 .18 (6H, m) , 7 . 33-7 . 39 (9H, m) , 7.57 (1H, d, J = 1.2 Hz), 8.07 (1H, d, J = 1.2 Hz). Production example 1127
(3-Iodo-l-trityl-lH-pyrazolo[3,4-c]pyridin-5-yl)carbamic acid tert-butyl ester
To a suspension of 1.0 g of 3-iodo-l-trityl-1H-pyrazolo[3,4-c]pyridine-5-carboxylic acid in tert-butanol were added 0.57 g of diphenylphosphoryl azide and 0 . 31 mL of triethylamine at room temperature, andheated at reflux for 6 hours. The solution was diluted with ethyl acetate, the organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified
and separated by silica gel column chromatography (ethyl acetate :n-hexane=l: 10) , to afford 455 mg of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 1.53 (9H, s) , 7.07 (1H, bs) , 7.15-7.19 (6H, m), 7.21 (1H, d, J=1.2Hz), 7.25-7.29 (9H, m), 7.84 (1H, bs) .
Production example 1128
{3-[(E)-2-(4-Fluorophenyl)vinyl]-1-trityl-1H-pyrazolo[3,4-c]pyridin-5-yl}carbamic acid tert-butyl ester
To a solution of 400 mg of (3-iodo-l-trityl-lH-pyrazolo[3,4-c]pyridin-5-yl)carbamic acid tert-butyl ester and 162 mg of 4-fluorostyrene in 2.0 mL of N,N-dimethylf ormamide were added 15 mg of palladium acetate, 40 mg of 2-(di-tert-butylphosphino)biphenyl and 0.46 mL of triethylamine at room temperature, and stirred at 80°C for 6 hours. After diluting with ethyl acetate, the organic layer was washed successively with saturated aqueous ammonium chloride and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the crude product was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:10-dichloromethane:n-hexane=l:1-ethyl acetate :n-hexane=l:3). The resultant crystals were washed with diethyl ether, to afford 270 mg of the title compound as colorless crystals.
1H-NMR ( 400 MHz, CDCl3 ) δ 1.55 (9H, s) , 7.04 (2H, t, J = 8.4 Hz), 7.08 (1H, bs), 7.20-7.30 (m, 16H), 7.31 (1H, d, J = 1.6
Hz), 7.37 (1H, d, J = 16.4 Hz), 7.51 (2H, dd, J = 8.4, 5.6 Hz),
8.32 (1H, bs)
Example 1129
3-[(E)-2-(4-Fluorophenyl)vinyl]-lH-pyrazolo[3, 4-c]pyridin-
5-ylamine
To a solution of 10 mg of {3-[(E)-2-(4-fluorophenyl)vinyl]-1-trityl-lH-pyrazolo[3, 4-c]pyridin-5-yl}carbamic acid tert-butyl ester in 0.5 mL of dichloromethane was added 0.2 mL of trifluoroacetic acid at room temperature, and stirred at this temperature for 10 minutes. Following concentration by nitrogen blowing, the residue was separated and purified by LC-MS, to afford the title compound. MS (ESI)m/z 255 MH+ Example 1130
N-{3- [ (E) -2- (4-Fluorophenyl) vinyl] -1H-pyrazolo [3, 4-c] -pyridin-5-ylJacetamide
To a solution of 10 mg of {3-[(E)~2-(4-fluorophenyl)vinyl]-1-trityl-lH-pyrazolo[3,4-c]pyridin-5-yl} carbamic acid tert-butyl ester obtainedby Production example 1128 in 0.2 mL of dichloromethane were successively added 7 ul of diisopropylethylamine at room temperature and 3 ul of acetyl chloride, and stirred at this temperature for a day. To this reaction solution was added 0.1 mL of trifluoroacetic acid at this temperature and stirred for 10 minutes. Following concentration by nitrogen blowing, the residue was separated and purified by LC-MS, to afford the title compound.
MS (ESI)m/z 297 MH+ Example 1131
The compounds of Examples 1132 and 1133 were synthesized from {3-[(E)-2-(4-fluorophenyl)vinyl]-1-trityl-lH-pyrazolo [3, 4-c]pyridin-5-yl}carbamicacid tert-butyl ester and commercially available acid chloride in accordance with the procedure of Example 1130. Example 1132
Cyclopropane carboxylic acid {3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-pyrazolo[3,4-c]pyridin-5-yl}amide MS (ESI)m/z 323 MH+ Example 1133 Furan-2-carboxylic acid
{3-[(E) -2-(4-fluorophenyl)-vinyl]-lH-pyrazolo[3, 4-c]pyridin -5-yl}amide MS (ESI)m/z 349 MH+ Example 1134
In accordance with the method of Example 598, 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl] -lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 or 6-fluoro-3-{(E)-2-(4-fluoro-phenyl)-vinyl}-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 559 and various kinds of hydrazide, the compounds of Examples 1135-1138 were obtained. Example 1135 (5-{ 6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-[1,3,4]oxadiazol-2-ylmethyl)-methyl-amine
MS (ESI)m/z 368 MH+
Example 1136
1-(5-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-[1,3,4]oxadiazol-2-yl)-cyclopropylamine
MS (ESI)m/z 380 MH+
Example 1137
(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-[1, 3, 4]oxadiazol-2-ylmethyl)-methyl-amine
MS (ESI)m/z 368 MH+
Example 1138
l-(5-{7-Fluoro-3-[{E)~2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-[1,3,4]oxadiazol-2-yl)-cyclopropylamine
MS (ESI)m/z 380 MH+
Production example 1139
Piperidin-1-yl-acetic acid hydrazide
3.86 g of piperidin-1-yl-acetic acid ethyl ester was dissolved in 40 mL of ethanol, added with 3.39 mL of hydrazine monohydrate, and stirred at 70°C for 5 hours. After allowing the solution to cool to room temperature, the solvent was evaporated, to afford 3.8 g of the title compound as pale yellow crystals.
1H-NMR (400 MHz, CDCl3) δ 1.38-1.4 8 (2H, m) , 1.51-1.63 (4H, m) , 2.42 (4H, brs), 3.02 (2H, s), 3.84 (2H, brs), 8.22 (1H, brs) Production example 1140 Pyridin-3-yl-acetic acid hydrazide
In accordance with Production example 1139, from 5.37 g of pyridin-3-yl-acetic acid ethyl ester, 6.0 g of the title compound was obtained as pale yellow crystals. 1H-NMR (400 MHz, DMSO-D6) δ 4.22 (2H, brs) , 7.32 (1H, ddd, J = 7.6, 4.8, 0.8 Hz) , 7.64-7.68 (1H, m) , 8.42-8.4 6 (2H, m) , 9.29 (1H, brs) Example 1141
In accordance with the method of Example 371 or 375, from 7-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383, and hydrazide obtainedby Production example 1140 or various kinds of hydrazide synthesized in accordance with Production examples 366-367, the compounds of Examples 1142-1145 were obtained. Example 114 2
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-[5-(piperidin-4-yl)-AH- [1,2,4]triazol-3-yl]-1H-indazole MS (ESI)m/z 407 MH+ Example 1143
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-[5-(pyridine-3-yl)methyl-4H- [1,2,4]triazol-3-yl]-lH-indazole MS (ESI)m/z 415 MH+ Example 1144
1-(5-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H- [l,2,4]triazol-3-yl)-cyclopentylamine MS (ESI)m/z 407 MH+
Example 1145
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-[5-(piperidin-4-yl)methyl-4H-[1,2,4]triazol-3-yl]-lH-indazole MS (ESI)m/z 421 MH+ Example 114 6
7-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5- [5-(piperidin-1-yl)methyl-4H-[l,2,4]triazol-3-yl]-1H-indazole
13 mg piperidin-1-yl-acetic acid hydrazide produced by Production example 1139 and 15 mg of
7-fluoro-3-{2-(4-fluorophenyl)-vinyl}-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383 were dissolved in 1 mL of butanol, and added with 30 ul of triethylamine. After stirring at 105°C for 8 hours, the solution was purification by LC-MS, to afford 2 . 08 mg of the title compound. MS (ESI)m/z 421 MH+ Example 1147
[(1S)-1-(5-{7-Fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-4H-[l,2,4]triazol-3-yl)-2-methyl-propyl]-methyl-amine
20.2 mg of [(15)-l-hydrazinocarbonyl-2-methyl-propyl]-methylcarbamic acid tert-butyl ester and 15 mg of
7-fluoro-3-{(E)-2-(4-fluorophenyl)-vinyl}-lH-indazole-5-car boxymidic acid ethyl ester hydrochloride obtained by Example 383 were reacted in accordance with Example 1146, the solvent was distilled of f, treated with 1 mL of 4N hydrogen chloride-ethyl
acetate for 3 hours, and then purified by LC-MS, to afford 5.31
mg of the title compound.
MS (ESI)m/z 409 MH+
Production example 114 8
N-Methyl-hydrazine carboxylic acid tert-butyl ester
10 mL of methyl hydrazine was dissolved in 90 mL of ethanol, and under stirring at ice-cooling, a solution of 41 g of di-tert-butyldicarbonate in 90 mL of ethanol was added dropwise over 45 minutes . After stirring at room temperature for 7 hours, the solvent was evaporated, to afford 24 . 7 g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 1.47 (9H, s), 3.05 (3H, s) , 4.05 (2H, brs)
Production example 114 9
N-benzyloxycarbonyl-N-methyl-hydrazine carboxylic acid tert-butyl ester
13.5 g of N-methyl-hydrazine carboxylic acid tert-butyl ester was dissolved in 90 mL of chloroform, added with 90 mL of IN sodium hydroxide aqueous solution and 28 mL of benzyl chloroformate, and stirred at room temperature for 6 days. Adding water, extracting with chloroform, and the resultant organic layer was washed with saturated brine and dried over magnesium sulfate. The solvent was evaporated, followed by purification by silica gel column chromatography (ethyl acetate :hexane=l:4), to afford 17.7 g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 1.42 (9H, s), 3.14 (3H, brs) , 5.17
(2H, s), 7.28-7.42 (5H, m)
Production example 1150
N-Methyl-hydrazine carboxylic acid benzyl ester hydrochloride
17.7 g of N'-benzyloxycarbonyl-N-methyl-hydrazine carboxylic acid tert-butyl ester was dissolved in 20 mL of ethyl acetate, added with 70 mL 4N hydrogen chloride-ethyl acetate solution, and stirred at room temperature for 4 hours. The solvent was evaporated, and the resulting crystals were washed with diethyl ether and filtered, to afford 11.4 g of the title compound as white crystals. 1H-NMR (400 MHz, DMSO-D6) δ2.72 (3H, s) , 5.19 (2H, s) , 7.32-7.44
(5H, m)
Production example 1151
Methyl-(N-methyl-hydrazinocarbonylmethyl)-carbamic acid
tert-butyl ester
2.1 g of (tert-butoxycarbonyl-methyl-amino)-acetic acid was dissolved in 150 mL of tetrahydrofuran, and 4.64 mL of triethylamine and 1.51 mL of isobutyl chloroformate were added under stirring at ice-cooling. After stirring at room temperature for 10minutes, N-methyl-hydrazine carboxylic acid benzyl ester hydrochloride was added and stirred at room temperature for 50 minutes. The reaction solution was added with water, extracted with ethyl acetate, and the resultant organic layer was washed with saturated brine and dried over magnesium sulfate. The solvent was evaporated, followed by
purification by silica gel column chromatography (ethyl
acetate :n-hexane=l:1), to afford 3.2 g of
N'- [2- (tert-butoxycarbonyl-methyl-amino) -acetyl] -N-methyl -
hydrazine carboxylic acid benzyl ester. Subsequently, by
conducting catalytic hydrogen reduction in accordance with
Production example 365, 1. 9 g of the title compound was obtained.
MS (ESI)m/z 240 MNa+
Example 1152
(5-{7-Fluoro-3-[(E)-2-(4-fluoro-phenyl)-vinyl]-1H-
indazol-5-yl}-2-methyl-2H- [1,2,4]triazol-3-ylmethyl)-
methylamine
In accordance with Example 1147, from methyl-(N-methyl-hydrazinocarbonylmethyl)-carbamic acid tert-butyl ester and 7-fluoro-3-{(E)-2-(4-
fluoro-phenyl)-vinyl}-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained by Example 383, the title compound was obtained. MS (ESI)m/z 381 MH+ Example 1153
5-(4,5-Dihydro-lH-imidazol-2-yl)-6-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-lH-indazole
To a suspension of 10 mg of 6-fluoro-3-[[E)-2-(4-fluorophenyl)vinyl]-1H-indazole-5-carboxydimic acid ethyl ester hydrochloride obtained by Example 559 in 0. 5 mL of ethanol was added 30 µl (1 mole in ethanol) of ethylenediamine at room temperature, andheated under reflux for 2 days . After filtering
out the insoluble substances, the residue was separated and purified by LC-MS, to afford the title compound. MS (ESI)m/z 325 MH+ Example 1154
In accordance with the procedure of Example 1153, from 6-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-lH-indazole-5-carboxydimic acid ethyl ester hydrochloride obtained by Example 559 or 7-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-1H-indazole-5-carboxydimic acid ethyl ester hydrochloride obtained by Example 383, and commercially available substituted ethylenediamine or substituted 1,3-diaminopropane, the compounds of Examples 1155-1165 were synthesized. Example 1155
6-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(l-methyl-4,5-dihydro-1H-imidazol-2-yl) -lH-indazole MS (ESI)m/z 339 MH+ Example 1156
6-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(5-methyl-4,5-dihydro-lH-imidazol-2-yl)-1H-indazole MS (ESI)m/z 339 MH+ Example 1157
6-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(1,4,5,6-tetrahydropyrimidin-2-yl)-lH-indazole MS (ESI)m/z 439 M+ Example 1158 6-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(1-
methyl-1,4,5,6-tetrahydropyrimidin-2-yl)-lH-indazole
MS (ESI)m/z 353 MH+
Example 1159
2-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-lH-indazole-5-
yl}-1,4,5,6-tetrahydropyrimidin-5-ol
MS (ESI)m/z 355 MH+
Example 1160
5-(4,5-Dihydro-lH-imidazol-2-yl)-7-fluoro-3-[(E)-2-(4-
fluorophenyl)vinyl]-lH-indazole
MS (ESI)m/z 325 MH+
Example 1161
7-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(l-methyl-4,5-
dihydro-2H-imidazol-2-yl) -1H-indazole
MS (ESI)m/z 339 MH+
Example 1162
7-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(5-methyl-4,5-
dihydro-lH-imidazol-2-yl)-lH-indazole
MS (ESI)m/z 339 MH+
Example 1163
7-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(1, 4 , 5, 6-
tetrahydropyrimidin-2-yl)-lH-indazole
MS (ESI)m/z 339 MH+
Example 1164
7-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(1-
methyl-1,4,5,6-tetrahydropyrimidin-2-yl)-lH-indazole
MS (ESI)m/z 353 MH+
Example 1165
2-{7-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-1H-
indazol-5-yl}-1,4,5,6-tetrahydropyrimidin-5-ol
MS (ESI)m/z 355 MH+
Example 1166
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid (2-oxopropyl) amide
100 mg of 3-[ (E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (2-hydroxylpropyl) amide obtained by Example 825 and 300 ul of triethylamine were dissolved in a mixed solvent of 4 mL dichloromethane/lmL dimethyl sulfoxide, added with 1 mL of a solution of 260 mg of pyridine sulfur trioxide complex in dimethyl sulfoxide under ice cooling, and the reaction solution was heated at 40°C for 3 hours. The reaction solution was diluted with water, extracted with ethyl acetate, washed with water, and then dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography (ethyl acetate .-methanol =100:3) , and then crystallized from ethyl acetate and diisopropyl ether, to afford 80 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 2.18 (3H, s), 3.98 (3H, s), 4.22 (2H, d, J = 5.5 Hz), 7.25 (2H, t, J = 8.8 Hz), 7.35 (1H, d, J = 8.4 Hz), 7.51 (1H, d, J = 16.4 Hz), 7.51 (1H, d, J = 16.4 Hz), 7.57 (1H, d, J = 16.4 Hz) , 7.72 (2H, dd, J = 5.6, 8.8 Hz) , 7.73 (1H, d, J = 8.4 Hz) , 8.64 (1H, bt, J = 5.5 Hz) , 13.4 8 (1H, bs) . Example 1167
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [2-hydroxy-3-(morpholin-4-yl)propyl]amide
100 mg of 3-[(E)-2-(4-fluorophenyl)-vinyl]-A-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234 and 130 mg of l-amino-3-(morpholine-4-yl)propane-2-ol were condensed in the similar method as described in Example 44, to afford 66 mg of the title compound as colorless needle crystals. 1H-NMR (400 MHz, CD3OD) δ 2.51 (2H, d, J = 6.4 Hz), 2.53-2.62 (4H, m) , 3.4 4 (1H, dd, J = 6.8, 13.6 Hz) , 3.68 (1H, dd, J = 4.4, 13.6Hz), 3.71 (4H, t, J=4.6Hz), 4.00 (3H, s) , 4.00-4.07 (1H, m), 7.14 (2H, t, J = 8.8 Hz), 7.34 (1H, d, J = 8.8 Hz), 7.54 (1H, d, J = 16.4 Hz), 7.59 (1H, d, J = 16.4 Hz), 7.64 (2H, dd, J = 5.6, 8.8 Hz), 7.87 (1H, d, J = 8.8 Hz). Example 1168
3-[(E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester
410 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 5-vinyl-2, 3-dihydrobenzofuran were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 50 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CDCl3) δ 1.44 (3H, t, J = 7.2 Hz), 3.26 (2H, t, J = 8.7 Hz), 4.05 (3H, s), 4.42 (2H, q, J = 7.2 Hz), 4.62 (2H, t, J = 8.7 Hz), 6.81 (1H, d, J = 8.4 Hz), 7.21 (1H, d, J = 8.6 Hz) , 7.36 (1H, bd, J= 8.4 Hz), 7.4 8 (1H, bs) , 7.50 (1H,
d, J = 16.4 Hz), 7.62 (1H, d, J = 16.4 Hz), 7.91 (1H, d, J =
8.6 Hz).
Example 1169
3-[ (E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid
50 mg of 3-[(E)-2-(2,3-dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester was treated in the similar method as described in Example 144, to afford 35 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, CD3OD) δ 3.25 (2H, t, J = 8.8 Hz), 4.02 (3H, s), 4.58 (2H, t, J = 8.8 Hz), 6.75 (1H, d, J = 8.4 Hz), 7.27 (1H, d, J = 8.6 Hz), 7.34 (1H, dd, J = 1.6, 8.4 Hz), 7.47 (1H, d, J = 16.4 Hz) , 7.51 (1H, bs) , 7.52 (1H, d, J = 16.4 Hz) , 7.88 (1H, d, J = 8.6 Hz). Example 1170
4-Methoxy-3-[ (E)-2-(4-methoxyphenyl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester
470 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 4-methoxystyrene were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 130 mg of the title compound as a pale yellow oil. 1H-NMR (400 MHz, CDCl3) δ 1.45 (3H, t, J = 7.2 Hz), 3.85 (3H, s), 4.05 (3H, s), 4.43 (2H, q, J = 7.2 Hz), 6.93 (2H, d, J = 8.8 Hz), 7.20 (1H, d, J = 8.8 Hz), 7.54 (2H, d, J = 8.8 Hz),
7.54 (1H; d, J = 16.4 Hz), 7.62 (1H, d, J = 16.4 Hz), 7.8 9 (1H,
d, J = 8.8 Hz).
Example 1171
4-Methoxy-3-[ (E)-2-(4-methoxyphenyl)-vinyl]-lH-indazole-5-
carboxylic acid
50 mg of 4-methoxy-3-[ (E)-2-(4-methoxyphenyl)-vinyl]-1H-indazole-5-carboxylic acid ethyl ester was treated in the similar method as described in Example 144, to afford 40 mg of the title compound as a colorless powder.
1H-NMR (400 MHz, DMSO-D6) δ 3.80 (3H, s), 3.96 (3H, s), 6.99 (2H, d, J = 8.6 Hz), 7.29 (1H, d, J = 8.6 Hz), 7.44 (1H, d, J = 16.4 Hz), 7.51 (1H, d, J= 16.4 Hz), 7.59 (2H, d, J=8.6Hz), 7.74 (1H, d, J = 8.6 Hz), 13.38 (1H, bs). Example 1172
3-[ (E)-2-(3,4-Dimethoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid ethyl ester
470 mg of 3-iodo-4-methoxy-l-trityl-lH-indazole-5-carboxylic acid ethyl ester obtained by Production example 276 and 300 mg of 3, 4-dimethoxystyrene were reacted in the similar method as described in Production example 181, followed by deprotection in the similar method as described in Example 16, to afford 120 mg of the title compound as a pale yellow powder. 1H-NMR (400 MHz, CDCl3) δ 1.45 (3H, t, J = 7.2 Hz), 3.93 (3H, s), 3.96 (3H, s), 4.05 (3H, s), 4.44 (2H, q, J =7.2 Hz), 6.90 (1H, d, J = 8.8 Hz), 7.15 (1H, dd, J = 1.6, 8.8 Hz), 7.16 (1H, bs) , 7.22 (1H, d, J = 8.8 Hz) , 7.53 (1H, d, J = 16.4 Hz) , 7.62
(1H, d, J = 16.4 Hz), 7.91 (1H, d, J = 8.8 Hz). Example 1173
3-[(E)~2-(3, 4-Dimethoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid
50 mg of 3-[ (E)-2-(3,4-dimethoxyphenyl)-vinyl]-4-methoxy-2H-indazole-5-carboxylic acid ethyl ester was treated in the similar method as described in Example 144, to afford 40 mg of the title compound as a colorless powder. 1H-NMR (400 MHz, DMSO-D6) δ 3.79 (3H, s), 3.85 (3H, s), 3.96 (3H, s), 7.00 (1H, d, J = 8.4 Hz), 7.17 (1H, dd, J = 2.0, 8.4 Hz), 7.23 (1H, d, J = 2.0 Hz), 7.29 (1H, d, J = 8.4 Hz), 7.45 (1H, d, J = 16.4 Hz), 7.51 (1H, d, J = 16.4 Hz), 7.74 (1H, d, J = 8.6 Hz), 13.39 (1H, bs) . Example 1174
After condensing various kinds of carboxylic acid obtained by Example 942, 955, 968, 1169, 1171 and 1173, and various kinds of amine in the similar method as described in Example 44, purification by LC-MS was conducted, to afford the compounds of Examples 1175-1195. Example 1175
4-Methoxy-3-[(E)-2-(4-methoxyphenyl)-vinyl]-lH-indazole-5-carboxylic acid cyclopropylmethyl amide MS (ESI) m/z 378 MH+ Example 1176
4-Methoxy-3-[(E)-2-(4-methoxyphenyl)-vinyl]-lH-indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 405 MH+
Example 1177
3-[ (E)-2-(4-Methoxyphenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid cyanomethylamide
MS (ESI) m/z 363 MH+
Example 1178
3-[ (E)-2-(4-Methoxyphenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid allylamide
MS (ESI) m/z 364 MH+
Example 1179
4-Methoxy-3-[ (E)-2-(3,4-dimethoxyphenyl)-vinyl]-1H-
indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 435 MH+
Example 1180
3-[(E)-2-(3,4-Dimethoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyanomethylamide
MS (ESI) m/z 393 MH+
Example 1181
3-[ (E)-2-(3,4-Dimethoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid allylamide
MS (ESI) m/z 394 MH+
Example 1182
3-[(E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (furan-2-ylmethyl) amide
MS (ESI) m/z 416 MH+
Example 1183
3-[(E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylmethyl amide
MS (ESI) m/z 390 MH+
Example 1184
3-[(E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid (thiophen-2-ylmethyl) amide
MS (ESI) m/z 432 MH+
Example 1185
3-[(E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 376 MH+
Example 118 6
3-[(E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid (pyridin-3-ylmethyl) amide
MS (ESI) m/z 427 MH+
Example 1187
3- [ (E)-2- (2, 3-Dihydrobenzofuran-5-yl) -vinyl] -4-methoxy-1H-
indazole-5-carboxylic acid (oxazol-2-ylmethyl) amide
MS (ESI) m/z 417 MH+
Example 118 8
3- [ (E)-2- (2, 3-Dihydrobenzofuran-5-yl) -vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyanomethylamide
MS (ESI) m/z 375 MH+
Example 118 9
3-[ (E)-2-(2,3-Dihydrobenzofuran-5-yl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid allylamide
MS (ESI) m/z 376 MH+
Example 1190
3-[ (E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyanomethylamide
MS (ESI) m/z 381 MH+
Example 1191
3-[(E)-2-(4-Fluoro-3-methoxyphenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid allylamide
MS (ESI) m/z 382 MH+
Example 1192
3-[(E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-1H-
indazole-5-carboxylic acid cyanomethylamide
MS (ESI) m/z 381 MH+
Example 1193
3-[ (E)-2-(3-Fluoro-4-methoxyphenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid allylamide
MS (ESI) m/z 382 MH+
Example 1194
3-[ (E)-2-(3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid cyanomethylamide
MS (ESI) m/z 369 MH+
Example 1195
3-[ (E)-2-{3,4-Difluorophenyl)-vinyl]-4-methoxy-lH-
indazole-5-carboxylic acid allylamide
MS (ESI) m/z 370 MH+
Production example 1196
Acetic acid 5-(morpholin-4-yl)methyl-furan-2-ylmethyl ester
Under nitrogen atmosphere, to a solution of 2.0 g of 5-acetoxymethyl-2-furanaldehyde and 1.1 mL of morpholine in 35 mL of 1,2-dichloroethane was added 4.0 g of sodium triacetoxy borohydride at room temperature, and stirred at this temperature for 3 hours. The reaction solution was diluted with dichloromethane, washed successively with saturated aqueous sodium hydrogen carbonate and saturated brine, and then dried over anhydrous magnesium sulfate. The solvent was evaporated, and the residue was purified and separated by silica gel column chromatography, to afford 2.3g of the title compound as a colorless oil.
1H-NMR (400 MHz, CDCl3) δ 2.08 (3H, s), 2.49 (4H, m) , 3.53 (2H, s), 3.73 (4H, m), 5.02 (2H, s), 6.19 (1H, d, J = 3.2 Hz), 6.34 (1H, d, J = 3.2 Hz). Production example 1197 [5-(Morpholin-4-yl)methyl-furan-2-ylj-methanol
In accordance with the method of Production example 773, from 2.2 g of acetic acid 5-(morpholin-4-yl)methyl-furan-2-ylmethyl ester, 1.4 g of the title compound was obtained as colorless crystals.
1H-NMR (400MHz, CDCl3)δ2.48 (4H, m), 3.52 (2H, s), 3.72 (4H, m) , 4.59 (2H, s) , 6.17 (1H, d, J = 3.2 Hz), 6.23 (1H, d, J = 3.2 Hz).
Production example 1198 4-(5-Azidomethyl-furan-2-ylmethyl)-morpholine
In accordance with the method of Production example 774, from 1.0 g of [5-(morpholin-4-yl)methyl-furan-2-yl]-methanol, 1.07 g of the title compound was obtained as a colorless oil. 1H-NMR (400 MHz, CDCl3)δ2.48 (4H, m) , 3.54 (2H, s), 3.73 (4H, m) , 4.29 (2H, s), 6.20 (1H, d, J = 3.2 Hz), 6.30 (1H, d, J = 3.2 Hz).
Production example 1199 C-[5-(Morpholin-4-yl)methyl-furan-2-yl]-methylamine
In accordance with the method of Production example 775, from 1.07 g of 4-(5-azidomethyl-furan-2-ylmethyl)-morpholine, 900 mg of the title compound was obtained as colorless oil. 1H-NMR (400MHz, CDCl3)δ2.48 (4H, m), 3.50 (2H, s), 3.74 (4H, m), 3.80 (2H, s), 6.06 (1H, d, J = 3.2 Hz), 6.13 (1H, d, J = 3.2 Hz).
Production example 1200
Acetic acid 5-(4-methyl-piperazin-l-ylmethyl)-furan-2-ylmethyl ester
In accordance with the method of Production example 1196, from 1.44 mL of 1-methylpiperazine, 2.1 g of the title compound was obtained as a pale yellow oil.
1H-NMR (400MHz, CDCl3)δ2.08 (3H, s), 2.29 (3H, s), 2.49 (8H, m) , 3.55 (2H, s), 5.02 (2H, s), 6.18 (1H, d, J =3.2 Hz), 6.33 (1H, d, J = 3.2 Hz) . Production example 1201 [5-(4-Methyl-piperazin-l-ylmethyl)-furan-2-yl]-methanol
In accordance with the method of Production example 773,
from 2.1 g of acetic acid 5-(4-methyl-piperazin-l-
ylmethyl) -furan-2-ylmethyl ester, 1.26 g of the title compound
was obtained as a colorless oil.
1H-NMR (400 MHz, CDCl3)δ2.29 (3H, s), 2.51 (8H, bs) , 3.54 (2H,
s), 4.57 (2H, s), 6.16 (1H, d, J = 3.2 Hz), 6.22 (1H, d, J =
3.2 Hz).
Production example 1202
1-(5-Azidomethyl-furan-2-ylmethyl)-4-methyl-piperazine
In accordance with the method of Production example 774, from 1.26 g of [5-(4-methyl-piperazin-l-ylmethyl)-furan-2-yl]-methanol, 1.0 g of the title compound was obtained as a colorless oil.
1H-NMR (400 MHz, CDCl3)δ2.29 (3H, s) , 2.49 (8H, bs) , 3.55 (2H, s), 4.28 (2H, s), 6.19 (1H, d, J = 3.2 Hz), 6.29 (1H, d, J = 3.2 Hz).
Production example 1203
C-[5-(4-Methyl-piperazin-l-ylmethyl)-furan-2-yl]-methylamine
In accordance with the method of Production example 775, from 1.0 g of 1-(5-azidomethyl-furan-2-ylmethyl)-4-methyl-piperazine, 920 mg of the title compound was obtained as a colorless oil.
1H-NMR (400MHz, CDCl3)δ2.28 (3H, s), 2.49 (8H, bs), 3.52 (2H, s), 3.79 (2H, s), 6.05 (1H, d, J = 3.2 Hz), 6.12 (1H, d, J = 3.2 Hz). Example 1204
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid obtained by Example 234 and amine obtained by Production example 1199 and 1203 were condensed in accordance with the method of Example 44, followed by purification by LC-MS, to afford the compounds of Examples 1205-1206. Example 1205
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [5-(4-methylpiperazin-l-ylmethyl)-furan-2-ylmethyl)-amide MS (ESI) m/z 505 MH+ Example 1206
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid [5-(morpholin-4-ylmethyl)-furan-2-ylmethyl]-amide MS (ESI) m/z 492 MH+ Example 1207
{3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carbonyl}-aminoacetic acid tert-butyl ester
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid obtained by Example 234 and 258 mg of glycine ter-butyl ester hydrochloride were dissolved in 20 mL of N,N-dimethylformamide, and 0.871 mL of diisopropylethylamine and 235 mg of 1-hydroxybenzotriazole were added, and stirred at room temperature for 10 minutes. After cooling to 0°C, 491 mg of WSC hydrochloride was added, and stirred
overnight at room temperature. After adding ice water to stop the reaction, the solution was extracted with ethyl acetate. The organic phase was washed with water and saturated brine and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 547 mg of the title compound as pale green crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.45 ( 9H, s ) , 3. 98 ( 5H, s ) , 7.25 ( 2H, t, J = 8.8 Hz ), 7.34 ( 1H, d, J = 8.4 Hz ), 7.51 ( 1H, d, J = 16.4 Hz ), 7.57 ( 1H, d, J = 16.4 Hz ), 7.68 -7.72 ( 3H, m ), 8.63 ( 1H, t, J = 6.0 Hz ) Example 1208
{3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carbonyl}-aminoacetic acid
472 mg of {3-[ (E) -2-(4-fluorophenyl)-vinyl]-4-methoxy-2H-indazole-5-carbonyl}-aminoacetic acid tert-butyl ester was dissolved in 5 mL of dichloromethane, 2.5 mL of trifluoroacetic acid was added, and stirred overnight at room temperature. The reaction solution was added with water, and extracted with a mixed solution of ethyl
acetate: tetrahydrofuran=l: 1. The organic layer was washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to give 384 mg of the title compound as a pale yellow solid.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 3.98 ( 3H, s ), 4.02 ( 2H, d, J = 6.0 Hz ), 7.25 ( 2H, t, J = 8.4 Hz ), 7.34 ( 1H, d, J = 8.4 Hz ) , 7.51 ( 1H, d, J = 16.0 Hz ) , 7.57 ( 1H, d, J = 16.0 Hz ) ,
7.70 - 7.72 ( 3H, m ), 8.62 ( 1H, t, J = 5.6 Hz ) Example 1209
In accordance with the method of Example 102, from {3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carbonyl} -aminoacetic acid obtained by Example 1208 and various kinds of amine, the compounds of Examples 1210-1224 were obtained. Example 1210
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [(cyclohexyl-methyl-carbamoyl)-methyl]-amide MS (ESI) m/z 465 MH+ Example 1211
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid (benzylcarbamoyl-methyl)-amide MS (ESI) m/z 459 MH+ Example 1212
3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid [2-oxo-2-(pyrrolidin-1-yl)-ethyl]-amide MS (ESI) m/z 423 MH+ Example 1213
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carboxylic acid [2-oxo-2-(piperidin-1-yl)-ethyl]-amide MS (ESI) m/z 437 MH+ Example 1214
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-carboxylic acid [2-(morpholin-4-yl)-2-oxo-ethyl]-amide
MS (ESI) m/z 439 MH+
Example 1215
4-[2- ({3-[(E)~2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-
indazole-5-carbonyl}-amino)-acetyl]-piperazine-1-carboxylic
acid tert-butyl ester
MS (ESI) m/z 538 MH+
Example 1216
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid [2-(4-methyl-piperazin-l-
yl)-2-oxo-ethyl]-amide
MS (ESI) m/z 452 MH+
Example 1217
3- [ (E) -2- (4-f luorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid diethylcarbamoylmethyl-amide
MS (ESI) m/z 425 MH+
Example 1218
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid cyclohexylcarbamoylmethyl-amide
MS (ESI) m/z 451 MH+
Example 1219
3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid cyclopentylcarbamoylmethyl-amide
MS (ESI) m/z 437 MH+
Example 1220
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-
carboxylic acid cyclobutylcarbamoylmethyl-amide
MS (ESI) m/z 423 MH+
Example 1221
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid cyclopropylcarbamoylmethyl-amide
MS (ESI) m/z 409 MH+
Example 1222
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid ethylcarbamoylmethyl-amide
MS (ESI) m/z 397 MH+
Example 1223
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazole-5-
carboxylic acid propylcarbamoylmethyl-amide
MS (ESI) m/z 411 MH+
Example 1224
3- [ (JE) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-lH-indazole-5-
carboxylic acid butylcarbamoylmethyl-amide
MS (ESI) m/z 425 MH+
Example 1225
3-f {E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazole-5-
carboxylic acid [2-oxo-2-(piperazin-1-yl)-ethyl]-amide
4-[2-({3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazole-5-carbonyl}-amino)-acetyl]-piperazine-1-carboxylic acid tert-butyl ester obtained by Example 1215 was dissolved in 1 mL of dichloromethane, added with 1 mL of trifluoroacetic acid, and stirred at room temperature for 2 hours. The solvent was evaporated, followed by purification by LC-MS, to afford
the title compound.
MS (ESI) m/z 438 MH+
Example 122 6
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-2H-indazole-5-
carboxylic acid (3-hydroxyisoxazol-5-ylmethyl)-amide
In accordance with the method of Example 102, 3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxyl lH-indazole-5-carboxylic acid obtained by Example 234 and 5-aminomethyl-isooxazol-3-ol, the title compound was obtained. MS (ESI) m/z 409 MH+ Production example 1227
1-Methanesulf onyloxymethyl-cyclopropane carboxylic acid ethyl ester
In accordance with the method described in the document (Tetrahedron Letters, £0, 5467 (1988)), a solution of 720 mg of 1-hydroxymethyl-cyclopropane carboxylic acid ethyl ester obtained from 1,1-cyclopropane dicarboxylic acid diethyl ester in l0 mL of dichloromethane was added with 1. 05 mL of triethylamine and 0.5 mL of methanesulfonyl chloride under ice cooling, and stirred at this temperature for 2 hours. After adding water, the reaction solution was extracted with ethyl acetate. The organic layer was washed successively with 1N hydrochloric acid, saturated brine, saturated aqueous sodium hydrogen carbonate and saturated brine. The organic layer was dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 1.04 g of the title compound as a pale yellow oil.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 1.11 ( 2H, dd, J = 4.0, 6.8 Hz ) , 1.18 ( 3H, t, J = 7.2 Hz ), 1.24 ( 2H, dd, J = 4.0, 6.8 Hz ), 3.18 ( 3H, s ), 4.09 ( 2H, q, J = 7.2 Hz ), 4.32 ( 2H, s ) Production example 1228 1-Dimethylaminomethyl-cyclopropane carboxylic acid ethyl ester
To a solution of 222 mg of 1-methanesulf onyloxymethyl-cyclopropane carboxylic acid ethyl ester in 5 mL of tetrahydrofuran was added 3 mL of 2M dimethyl amine in tetrahydrofuran, and stirred at room temperature for 3 days . The solvent was distilled off, and the residue was added with 2 mL of IN hydrochloric acid, and washed with diethyl ether. The aqueous layer was added with 10% potassium carbonate aqueous solution, and extracted with ethyl acetate. The extracted layer was washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 56 mg of the title compound as a pale yellow oil. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.79 ( 2H, dd, J = 3. 6, 6.8 Hz ) , 1.07 ( 2H, dd, J = 3.6, 6.8 Hz ), 1.16 ( 3H, t, J = 7.2 Hz ), 2.13 ( 6H, s ), 2.46 ( 2H, s ) 4.03 ( 2H, q, J = 7.2 Hz ) Production example 1229 1-Dimethylaminomethyl-cyclopropane carboxylic acid
To a solution of 222 mg of 1-dimethylaminomethyl-cyclopropane carboxylic acid ethyl ester in 0.4 mL of ethanol was added 0.1 mL of 5N sodium hydroxide aqueous solution, and stirred overnight at room temperature. The reaction solution was added with 0.5 mL of IN hydrochloric
acid, and the solvent and water were evaporated, to give 78 mg
of a mixture of the title compound and sodium chloride as a white
powder.
1H-NMR ( 400 MHz, D2O ) δ 0.83 ( 2H, br s ) , 1.20 ( 2H, br s ),
2.86 ( 6H, s ), 3.16 ( 2H, s )
Production example 1230
1-[(tert-Butoxycarbonyl-methyl-amino)-methyl]-cyclopropane
carboxylic acid ethyl ester
To a solution of 222 mg of 1-methanesulf onyloxymethyl-cyclopropane carboxylic acid ethyl ester obtained by Production example 1227 in 2 mL of tetrahydrofuran was added 3 mL of 2M methylamine in tetrahydrofuran, and stirred at room temperature for 2 days. The reaction solution was added with 1.5 mL of 2N hydrochloric acid, and then washed with diethyl ether. The aqueous layer was added with 10% potassium carbonate aqueous solution, the water distilled off, and extracted with ethyl acetate. The extracted layer was added with 114 mg of di-tert-butyldicarbonate, and stirred for 1 hour. The reaction solution was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was evaporated, to afford 105 mg of the title compound as a colorless oil. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.91 ( 2H, br s ) , 1.11 ( 2H,br s ), 1.16 ( 3H, t, J = 7.2 Hz ), 1.39 ( 9H, s ), 2.80 ( 3H, br s ), 3.50 ( 2H, s), 4.05 ( 2H, q, J = 7.2 Hz ) Production example 1231
1-[(tert-Butoxycarbonyl-methyl-amino)-methyl]-cyclopropane carboxylic acid
In accordance with the method of Production example 651, from 105 mg of
1-[(tert-butoxycarbonyl-methyl-amino)-methyl]-cyclopropane carboxylic acid ethyl ester, 50 mg of the title compound was obtained as a colorless oil.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 0.85 ( 2H, br s ) , 1.09 ( 2H,br s ), 1.38 ( 9H, s ), 2.78,2.82 ( 3H, each s ), 3.48 ( 2H, s ), 12.30 ( 1H, br s ) Example 1232
Various kinds of amine obtained by Production examples 182, 643, 645 and 649, and various kinds of carboxylic acid obtained by Production examples 1229 and 1231 were amidated in the manner described in Example 183, deblocked, and purified by LC-MS [1%-100% gradient], to afford the compounds of Examples 1233-1240. Example 1233
1-Dimethylaminomethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 397 MH+ Example 1234
Dimethylaminomethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 397 MH+
Example 1235
1-Dimethylaminomethyl-cyclopropane carboxylic acid
{ 6-f luoro-3- [ (E) -2- (2-f luorophenyl) -vinyl] -1H-indazol-5-
yl}-amide
MS (ESI) m/z 397 MH+
Example 1236
1-Dimethylaminomethyl-cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-1H-indazol-5-
yl}-amide
MS (ESI) m/z 385 MH+
Example 1237
1-Methylaminomethyl-cyclopropane carboxylic acid
{ 6-fluoro-3- [ (E) -2- (4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-amide
MS (ESI) m/z 383 MH+
Example 1238
1-Methylaminomethyl-cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 383 MH+
Example 1239
1-Methylaminomethyl-cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(2-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 383 MH+
Example 1240
1-Methylaminomethyl-cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(thiophen-2-yl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 371 MH+
Production example 1241
6-Fluoro-3-[(E)-2-(3,4-difluorophenyl)-vinyl]-5-nitro-l-
trityl-lH-indazole
600 mg of 6-fluoro-3-iodo-5-nitro-l-trityl-1H-indazole obtained by Production example 747 and 305 mg of 1,2-difluoro-4-vinylbenzene was dissolved in 10 mL of 1,4-dioxane. The resultant solution was added with 56 mg of bis (tri-tert-butylphosphine)palladium(0) and 408 ul of diisopropylethylamine, and refluxed overnight under nitrogen atmosphere. After cooling to room temperature, the reaction mixture was added with silica gel, and the solvent was evaporated, followed by purification by silica gel column chromatography, to afford 513 mg of the title compound as yellow crystals. 1H-NMR ( 400 MHz, DMSO-D6 ) δ 6.13 ( 1H, d, J = 12.4 Hz ), 7.22 ( 6H, d, J = 7.6 Hz ), 7.34 - 7.49 ( 11H, m ), 7.53 ( 1H, d, J = 8.4 Hz ), 7.71 ( 1H, d, J = 16.8 Hz ), 7.95 ( 1H, t, J = 10.4 Hz ), 9.16 ( 1H, d, J = 7.2 Hz ) Production example 1242
6-Fluoro-3-[(E)-2-(3,4-difluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine
In accordance with the method of Production example 182,
from 513 mg of
6-fluoro-3-[(E)-2-(3,4-difluorophenyl)-vinyl]-5-nitro-l-tri
tyl-lH-indazole, 150 mg of the title compound was obtained as
yellow crystals.
1H-NMR ( 400 MHz, DMSO-D6 ) δ 4. 98 ( 2H, bs ) , 5. 91 ( 1H, d, J
= 11.6 Hz ), 7.18 - 7.45 ( 20H, m ), 7.83 ( 1H, dd, J = 8.4,
14.0 Hz )
Example 1243
6-Fluoro-3-[(E)-2-(3,4-difluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine obtained by Production example 1242 and various kinds of carboxylic acid were treated in the similar method as described in Example 183, to afford the compounds of Examples 1244-1256. Example 1244
Cyclopropane carboxylic acid {3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 358 MH+ Example 1245
1-Hydroxycyclopropane carboxylic acid {3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 374 MH+ Example 1246
l-Acetyl-piperidine-4-carboxylic acid {3-[(E)-2-(3,4-dif luorophenyl) -vinyl] -6-f luoro-2H-indazol-5-yl}-amide MS (ESI) m/z 443 MH+ Example 1247
Tetrahydrofuran-3-carboxylic acid {3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-1H-indazol-5-yl}-amide MS (ESI) m/z 388 MH+ Example 1248
1-Methylaminocyclopropane carboxylic acid
{3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazole-5-yl}-amide MS (ESI) m/z 387 MH+ Example 124 9
N-{3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 414 MH+ Example 1250
Furan-2-carboxylic acid {3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-1H-indazol-5-yl}-amide MS (ESI) m/z 384 MH+ Example 1251
2-Cyclopropyl-JV-{3- [ (E) -2- (3, 4-dif luorophenyl) -vinyl] -6-fluoro-lH-indazol-5-yl}-acetamide MS (ESI) m/z 372 MH+ Example 1252
(2S,4R)-4-Hydroxy-pyrrolidine-2-carboxylic acid {3-[{E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 403 MH+ Example 1253

N-{3-[(E)-2-(3,4-Difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-2-(morpholin-4-yl)-acetamide MS (ESI) m/z 417 MH+ Example 1254
Piperidine-4-carboxylic acid {3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 401 MH+ Example 1255
(25)-l-Methyl-pyrrolidine-2-carboxylic acid {3-[(E)-2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-amide MS (ESI) m/z 401 MH+ Example 1256
(2S)-2-Amino-N- {3-[ (E) -2-(3,4-difluorophenyl)-vinyl]-6-fluoro-lH-indazol-5-yl}-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 429 MH+ Example 1257
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-l-trityl-1H-indazol-5-ylamine synthesized by Production example 182 and various kinds of carboxylic acid were treated in the similar method as described in Example 183, to afford the compounds of Examples 1258-1260. Example 1258
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-2-methoxy-2-(thiophen-2-yl)-acetamide MS (ESI) m/z 426 MH+ Example 1259
Azetidine-3-carboxylic acid {6-fluoro-3-[(E)-2-(4-
fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 355 MH+
Example 1260
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-2-(piperazin-1-yl)-acetamide
MS (ESI) m/z 398 MH+
Production example 1261
4-Bromo-5-fluoro-2-methylphenylamine
To a solution of 49.0 g of 5-fluoro-o-toluidine in 600 mL of dichloromethane was added 69.7 g of N-bromosuccinimide at 0°C, and stirred at this temperature for an hour. Then sodium thiosulf ate aqueous solution was added, and the reaction solution was evaporated and diluted with ethyl acetate. The organic layer was washed successively with water and saturated brine and dried over anhydrous magnesium sulfate. Then the solvent was evaporated, and the precipitated crystals were washed with diethyl ether :n-hexane=l:10, to afford 81.6 g of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 2.09 (3H, s), 3.69 (2H, bs), 6.44 (1H, d, J = 10.4 Hz), 7.14 (1H, dd, J = 7.6, 0.8 Hz). Production example 1262 N-(4-Bromo-5-fluoro-2-methylphenyl)acetamide
73.6 mL of acetic anhydride was added with 80.0 g of 4-bromo-5-f luoro-2-methylphenylamine at room temperature, and stirred at this temperature for 10 minutes. Excess acetic
anhydride was distilled off under reduced pressure, and the
precipitated crystals were washed with water, to afford 77.6
g of the title compound as colorless crystal.
1H-NMR (400 MHz, CDCl3) δ 2.21 (6H, s), 6.92 (1H, bs), 7.32 (1H,
d, J = 7.6 Hz), 7.90 (1H, d, J = 10.4 Hz).
Production example 1263
1-(5-Bromo-6-fluoro-indazol-1-yl)ethanone
To a solution of 77.0 g of N- (4-bromo-5-fluoro-2-methylphenyl) acetamide in 930 mL of toluene were added 93.0 mL of acetic anhydride and 37.0 g of potassium acetate at room temperature, and the resultant solution was heated at 90°C, slowly added dropwise with 67 . 0 mL of isoamyl nitrite, and stirred at this temperature for 3 hours. After diluting with ethyl acetate, washing successively with water and saturated brine, and drying over anhydrous magnesium sulfate, the solvent was evaporated. The precipitated crystals were washed with diethyl ether: n-hexane=l: 10, the filtrate concentrated and purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=0:100-1: 5) and finally combined with the previous crystals, to afford 22.5 g of the title compound as pale brown crystals.
1H-NMR (400 MHz, CDCl3) 6 2.78 (3H, s), 7.93 (1H, d, J = 6.4 Hz), 8.05 (1H, d, J = 0.8 Hz), 8.23 (1H, dd, J = 4.8, 0.8 Hz). Production example 1264 5-Bromo-6-fluoro-1H-indazole
To a solution of 22.5 g of 1- (5-bromo-6-fluoro-indazol-1-
yl)ethanone in 250 mL of ethanol was added 20.0 mL of 5N sodium hydroxide aqueous solution at room temperature, and stirred at this temperature for 5 minutes. After neutralizing in 5N hydrochloric acid aqueous solution, the solution was diluted with ethyl acetate. The organic layer was successively washed with water and saturated brine, dried over anhydrous magnesium sulfate, and then the solvent was evaporated. The residue was purified and separatedby silica gel column chromatography (ethyl acetate :n-hexane=l:3), to afford 16.7 g of the title compound as pale brown crystals.
1H-NMR (4 00 MHz, CDCl3) δ 7.2 4-7.27 (1H, m) , 7. 94-7. 97 (1H, m) , 8.01 (1H, d, J = 0.8 Hz), 10.26 (1H, bs) . Production example 1265 5-Bromo-6-fluoro-3-iodo-lH-indazole
To a solution of 10.0 g of 5-bromo-6-fluoro-1H-indazole in 150 mL of N,N-dimethylformamide was added 10.5 g of N-iodosuccinimide at room temperature, and stirred at 70°C for 2 days . Then the solution was diluted with ethyl acetate, washed successively with aqueous ammonium chloride and saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated. The crystals precipitated by adding diethyl ether to the residue were collected by filtration. The filtrate was further concentrated and purified and separated by silica gel column chromatographsy (ethyl acetate :n-hexane=l:10-1:5) and combined with the previous crystals, to afford 14.5 g of the title compound as colorless crystals.
1H-NMR (400 MHz, DMSO-D6) δ 7.61 (1H, d, J =9.2 Hz), 7.75 (1H,
d, J = 6.4 Hz), 13.75 (1H, bs).
Production example 1266
5-Bromo-6-fluoro-3-iodo-l-trityl-lH-indazole
To a solution of 3.0 g of 5-bromo-6-fluoro-3-iodo-1H-indazole in N,N-dimethylformamide in 150 mL was added 0. 42 g of sodium hydride at room temperature, and the resultant solution was stirred for 15 minutes, added with 2.45 g of trityl-chloride, and stirred at this temperature for 1 hour. After adding water and diluting with ethyl acetate, washing successively with aqueous ammonium chloride and saturated brine and drying over anhydrous magnesium sulfate, the solvent was evaporated. The residue was purified and separated by silica gel column chromatography (ethyl acetate : n-hexane=l: 30-1: 5) , and the resultant product was added with n-hexane. Then the precipitated crystals were washed with diethyl ether :n-hexane=l:10, to afford 3.79 g of the title compound as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 6.07 (1H, d, J = 9.2 Hz), 7.14-7.20 (6H, m) , 7.26-7.31 (9H, m), 7.64 (1H, d, J = 6.4 Hz). Production example 1267
5-Bromo-6-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-1-trityl-lH-indazole
A solution of 3.0 g of 5-bromo-6-fluoro-3-iodo-l-trityl-1H-indazole, 628 mg of 4-fluorostyrene, 115 mg of palladium acetate, 307 mg of
2-(di-ter-butylphosphino)biphenyl and 3 . 60 mL of triethylamine
in 2V, N,N-dimethylformamide was stirred at 70°C for a day. After
diluting with ethyl acetate, washing successively with aqueous
ammonium chloride and saturated brine, and drying over anhydrous
magnesium sulfate, the solvent was evaporated. The residue was
purified and separated by silica gel column chromatography (ethyl
acetate : n-hexane=l: 100) , to afford 2.2 g of the title compound
as colorless crystals.
1H-NMR (400 MHz, CDCl3) δ 6.14 (1H, d, J = 10.0 Hz), 7.06 (2H,
t, J =8.8 Hz), 7.18-7.24 (17H, m) , 7.51 (2H, dd, J = 8.8, 6.4
Hz), 8.13 (1H, d, J = 6.8 Hz).
Production example 12 68
l-{5-(l-Acetyl-1H-pyrazol-4-yl)-6-fluoro-3-[(E)-2-(4-
fluorophenyl)vinyl]-lH-indazol-1-yl}ethanone
A solution of 40 mg of 5-bromo-6-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-1-trityl-lH-indazole, 29.4 mg of l-tritylpyrazole-4-boronic acid, 4 mg of
tetrakis(triphenylphosphine)palladium(0) and 32.8 mg of barium hydroxide octahydrate in a mixture of dimethoxyethane: water=0 . 6 mL: 0.1 mL was stirred at 80°C for a day. The solution was diluted with ethyl acetate and water, and the organic layer was washed with saturated brine and dried over anhydrous magnesium sulfate. Then the solvent was evaporated. A solution of the crude product in l m Lof dichloromethane was added with 0 . 2mL of trifluoroacetic acid at room temperature, and stirred at this temperature for 1 hour. The reaction solution was poured to aqueous sodium
hydrogen carbonate, and extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the then the solvent was evaporated. A solution of the crude product in 1 mL of tetrahydrofuran and 0.5 mL of diisopropylethylamine was added with 0.1 mL of acetic anhydride at room temperature and stirred at this temperature for a day. The reaction solution was evaporated, and the residue was purified and separated by silica gel column chromatography (ethyl acetate :n-hexane=l:3-1:1), to afford 13 mg of the title compound as colorless crystals. 1H-NMR (400 MHz, CDCl3) δ 2.78 (3H, s), 2.82 (3H, s) , 7.14 (2H, t, J = 8.8 Hz), 7.26 (1H, d, J = 16.4 Hz), 7.61-7.66 (3H, m), 8.05 (1H, d, J = 6.8 Hz), 8.14 (1H, s), 8.29 (1H, d, J = 11.2 Hz), 8.67 (1H, s) . Example 1269
6-Fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-5-(lH-pyrazolo-4-yl)-1H-indazole
To a solution of 13 mg of l-{5- (l-acetyl~lH-pyrazol-4-yl)-6-fluoro-3-[(E)-2-(4-fluorophenyl)vinyl]-1H-indazol-1-yl}ethanone in 1 mL of ethanol was added 0.2 mL of 5N sodium hydroxide aqueous solution at room temperature, and stirred at this temperature for 10 minutes. The solution was neutralized with 5N hydrochloric acid aqueous solution and diluted with ethyl acetate. After washing the organic layer with saturated brine and drying over anhydrous magnesium sulfate, the solvent was evaporated. The residue was purified and separated by silica
gel column chromatography (ethyl acetate :n-hexane=l:3-1:1), to afford 7.09 mg of the title compound as colorless crystal. MS (ESI)m/z 323 MH+ Example 1270
The compounds of Examples 1271 and 1272 were obtained according to the method of Example 1146 from 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained in Example 559 and the hydrazides obtained in Production Examples 1139 and 1140. Example 1271
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-[5-(piperidin-1-yl)-4H- [1,2,4]triazol-3-yl]-lH-indazole MS (ESI)m/z 421 MH+ Example 1272
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-5-[5-(pyridin-3-yl)methyl-4H- [1,2,4]triazol-3-yl]-lH-indazole MS (ESI)m/z 415 MH+ Example 1273
The compounds of Examples 1274 and 1275 were obtained according to the method of Example 1147 from 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazole-5-carboxymidic acid ethyl ester hydrochloride obtained in Example 559 and the hydrazides synthesized according to Production Examples 366 and 367.
Example 1274 6-Fluoro-3-[(E) -2-(4-fluorophenyl)-vinyl]-5-[5-
(piperidin-4-yl)-AH-[1,2, 4]triazol-3-yl]-1H-indazole MS (ESI)m/z 407 MH+ Example 1275
6-Fluoro-3-[(E) -2-(4-fluorophenyl)-vinyl]-5-[5-(piperidin-4-yl)methyl-4H-[1,2,4]triazol-3-yl]-1H-indazole MS (ESI)m/z 421 MH+ Production Example 1276
2-Chloro-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-yl}-acetamide
400 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-ylamine synthesized in Production Example 182 was dissolved in 20 ml of chloroform, 20 ml of a saturated aqueous solution of sodium hydrogen carbonate and 124ul of chloroacetyl chloride were added thereto at 0°C, and then the resulting mixture was stirred at room temperature overnight. The organic layer was washed with water and saturated brine, dried over anhydrous magnesium sulfate, and then the solvent was evaporated, to give 495 mg of the title compound as a yellow solid.
1H-NMR (400MHz, DMSO-D6) δ 4.35 (2H, s), 6.05 (1H, d, J=12.0Hz), 7.19-7.23 (8H, m) , 7.28-7.39 (10H, m) , 7.44 (1H, d, J=16.8Hz), 7.75 (2H, dd, J=5.6, 9.2Hz), 8.49 (1H, d, J=7.6Hz) Example 1277
To a suspension of 10 mg of 2-chloro-N-{ 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-l-trityl-lH-indazol-5-yl}-acetamide obtained in Production Example 1276 in 1 ml of
acetonit rile was added a previously prepared solutions of various
amines in 85 µl of 1. OM N,N-dimethylformamide, followed by
stirring at 60°C for 5 hours . The solvent was removed by nitrogen
stream and the obtained solids were dissolved in 1 ml of
dichloromethane, then 1 ml of trifluoroacetic acid was added
thereto and the solutions were stirred for 2 hours at room
temperature. The solvent was evaporated by nitrogen stream and
the obtained crude products were purified by LC-MS, to give the
compounds of Examples 1278 to 1287.
Example 1278
N-{6-Fluoro-3-[(E) -2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-(4-methyl-piperazin-l-yl)-acetamide
MS (ESI) m/z 412 MH+
Example 127 9
2-(Azetidin-1-yl)-N-{6-fluoro-3-[(E)-2- (4-fluorophenyl) -
vinyl]-lH-indazol-5-yl}-acetamide
MS (ESI) m/z 369 MH+
Example 1280
N-{6-Fluoro-3- [ (E) -2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-(pyrrolidin-1-yl)-acetamide
MS (ESI) m/z 383 MH+
Example 1281
N-{6-Fluoro-3-[(E) -2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-(piperidin-1-yl)-acetamide
MS (ESI) m/z 397 MH+
Example 1282
2-Cyclopropylamino-N-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-
vinyl] -1H-indazol-5-yl}-acetamide
MS (ESI) m/z 369 MH+
Example 1283
N-{ 6-Fluoro-3-[(E)-2-(4-fluorophenyl) -vinyl] -1H-indazol-5-
yl}-2-(imidazol-1-yl)-acetamide
MS (ESI) m/z 380 MH+
Example 1284
2-Benzylamino-N-{ 6-f luoro-3- [ (E) -2- (4-f luorophenyl) -
vinyl]-1H-indazol-5-yl}-acetamide
MS (ESI) m/z 419 MH+
Example 1285
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-
yl}-2-(pyridin-2-ylamino)-acetamide
MS (ESI) m/z 406 MH+
Example 1286
N-{6-Fluoro-3-[{E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-(pyridin-3-ylamino)-acetamide
MS (ESI) m/z 406 MH+
Example 1287
N-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-2-(pyridin-4-ylamino)-acetamide
MS (ESI) m/z 406 MH+
Production Example 1288
1-Hydroxymethyl-cyclopropane carboxylic acid {6-fluoro-3-
[{E)-2-(4-fluorophenyl)-vinyl]-l-trityl-lH-indazol-5-yl}-
amide
513 mg of 6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-1H-indazol-5-yl-amine obtained in Production Example 182 and 345 mg of 1-(tert-butyl-dimethyl-silanyloxymethyl)-cyclopropane carboxylic acid obtained in Production Example 653 were amidated according to the method of Production Example 183. Next, the tert-butyl-dimethyl-silyl group was removed by treatment with 2 ml of a 1M solution of tetra-n-butyl ammonium fluoride in tetrahydrofuran. Next, the crystals obtained by treatment with 0 . 5 ml of a 5N aqueous solution of sodium hydroxide were recrystallized using ethyl acetate-diisopropyl ether, to give 400 mg of the title compound as pale yellow crystals. 1H-NMR (400MHz, DMSO-D6) δ 0.76 (2H, dd, J=3.6, 6.8Hz), 1.07 (2H, dd, J=3.6, 6.8Hz), 3.61 (2H, d, J=5.2Hz), 5.74 (1H, t, J=5.2Hz), 6.05 (1H, d, J=12.0Hz), 7.16-7.24 (8H, m) , 7.26-7.44 (11H, m), 7.73 (2H, dd, J=5.6, 8.8Hz), 8.68 (1H, d, J=7.6Hz), 9.80 (1H, s) Production Example 128 9
l-{6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-1H-indazol-5-ylcarbamoyl}-cyclopropyl methyl methanesulfonate
100 mg of 1-hydroxymethyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-lH-indazol-5-yl}-amide was mesylated according to the method of Production Example 1227, to give 98 mg of the title compound as pale yellow crystals. 1H-NMR (400MHz, DMSO-D6) δ 1.07 (2H, dd, J=4.4, 6.8Hz), 1.33
(2H, dd, J=3.6, 6.8Hz), 3.18 (3H, s), 4.44 (2H, s), 6.04 (1H, d, J=11.6Hz), 7.16-7.24 (8H, m) , 7.28-7.40 (10H, m) , 7.44 (1H, d, J=16.8Hz), 7.76 (2H, dd, J=5.6, 8.8Hz), 8.20 (1H, d, J=7.2Hz), 9.16 (1H, s) Example 1290
A solution of l-{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-l-trityl-1H-indazol-5-yl-carbamoyl}-cyclopropyl methyl methanesulfonate in N-methyl-2-pyrrolidone was dispensed in test tubes, and 5 equivalents of various amines were added thereto, followed by stirring at room temperature for 4 days. After adding 0. 1N hydrochloric acid to the reaction mixture, it was extracted with ethyl acetate and the solvent was evaporated. Then, the residue was deprotected under deprotecting conditions according to Example 183, and purified by LC-MS (1% to 100% gradient) , to give the compounds of Examples 1291 to 1296. Example 1291
1-(Morpholin-4-yl)methyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide
MS (ESI) m/z 439 MH+ Example 1292
1-(Piperidin-l-yl)methyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 437 MH+
Example 1293
1-(Pyrrolidin-l-yl)methyl-cyclopropane carboxylic acid
{6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-
yl}-amide
MS (ESI) m/z 423 MH+
Example 1294
1-(4-Hydroxy-piperidin-l-yl)methyl-cyclopropane carboxylic
acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-amide
MS (ESI) m/z 453 MH+
Example 1295
1- (4-Methyl-piperazin-l-yl)methyl-cyclopropane carboxylic
acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-amide
MS (ESI) m/z 452 MH+
Example 1296
1-(4-Acetyl-piperazin-l-yl)methyl-cyclopropane carboxylic
acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-
indazol-5-yl}-amide
MS (ESI) m/z 480 MH+
Production Example 1297
(2S,AS)-4-(tert-Butyl-dimethyl-silyloxy)-pyrrolidin-1,2-
dicarboxylic acid 1-tert-butyl ester 2-methyl ester
4.97g of commercially available 1-tert-butyl 2-methyl {2S,4S)-4-hydroxy-pyrrolidin-l,2-dicarboxylic acid 1-tert-butyl ester 2-methyl ester was silyl etherif ied according
to the method of Production Example 652, to give 5.37g of the
title compound as a colorless oil.
1H-NMR (400MHz, DMSO-D6) δ 0.01, 0.03 (6H, each s), 0.82, 0.84
(9H, eachs), 1.34, 1.4 0 (9H, each s), 1.85-1.92 (1H, m) , 2.2 6-2.42
(1H, m) , 3.07-3.14 (1H, m) , 3.48-3.57 (1H, m) , 3.59, 3.62 (3H,
each s), 4.24-4.33 (1H, m), 4.36-4.44(lH,m)
Production Example 1298
(2S,AS)-4-(tert-Butyl-dimethyl-silyloxy)-pyrrolidin-1,2-
dicarboxylic acid 1-tert-butyl ester
1.07g of (2S,4S)-4-(tert-butyl-dimethyl-silyloxy)-pyrrolidin-1,2-dicarboxylic acid 1-tert-butyl ester 2-methyl ester was hydrolyzed according to the method of Production Example 651, to give 352 mg of the title compound as white crystals. 1H-NMR (400MHz, DMSO-D6) δ 0.03 (6H, s), 0.83 (9H, s) , 1.34, 1.4 0 (9H, each s) , 1.8 0-1.88 (1H, m) , 2.2 8-2.42 (1H, m) , 3.03-3.10 (1H, m), 3.49-3.58 (1H, m), 4.10-4.19 (1H, m), 4.33-4.44 (1H, m) , 12.38 (1H, br s) Production Example 1299 1-Acetyl-cyclopropane carboxylic acid
426 mg of 1-acetyl-cyclopropane carboxylic acid methyl
ester obtained from methyl acetoacetate using a known method
(Synthetic Commun., 26, 525 (1996)) was hydrolyzed according
to the method of Production Example 651, to give 337 mg of the
title compound as a colorless oil.
1H-NMR (400MHz, DMSO-D6) δ 1.28-1.35 (4H, m) , 2.36 (3H, s) , 12.88 (1H, br s)
Example 1300
6-Fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1-trityl-1H-indazol-5-yl-amine obtained in Production Example 182 and the two carboxylic acids obtained in Production Examples 1298 and 1299 were amidated and deprotected according to the method of Example 183, and then the products were purified by LC-MS (1% to 100% gradient), to give the compounds of Examples 1301 and 1302.
Example 1301
(2S,AS)-4-Hydroxy-pyrrolidine-2-carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-lH-indazol-5-yl}-amide MS (ESI) m/z 385 MH+ Example 1302
1-Acetyl-cyclopropane carboxylic acid {6-fluoro-3-[(E)-2-(4-fluorophenyl)-vinyl]-1H-indazol-5-yl}-amide MS (ESI) m/z 382 MH+ Production Example 1303 Amino-phenyl acetonitrile
To a 0.3 ml aqueous solution of 46 mg of sodium cyanide and 55 mg of ammonium chloride was added a solution of 100 mg of benzaldehyde in 0 . 3 ml of methanol, and the resulting mixture was stirredat room temperature for 5 hours . After addingaqueous solution of ammonium chloride to the reaction mixture, it was extracted with ethyl acetate twice. The organic layer was washed successively with a saturated aqueous solution of ammonium chloride and saturated brine, dried over anhydrous magnesium
sulfate, and the solvent was evaporated. Then, the crude product
was purified and separated by LC-MS, to give 44 . 2 mg of the title
compound as colorless crystals.
1H-NMR (400MHz, CD3OD) δ5.80 (1H, s) , 7.52-7.59 (3H, m) , 7.61-7.67
(2H, m).
Production Example 1304
Amino-pyridin-2-yl-acetonitrile
2.3 mg of the title compound was obtained from 100.7 mg of 2-pyridinecarboxaldehyde according to the method of Production Example 1303.
1H-NMR (4 00MHz, CD3OD) δ 5.92 (1H, s) , 7.55 (1H, dd, J=4.4, 7.6Hz) , 7.70 (1H, d, J=7.6Hz), 7.99 (1H, ddd, J=1.6, 7.6, 7.6Hz), 8.71 (1H, d, J=4.4Hz). Production Example 1305 3-Bromo-piperidin-2-one
Based on a method disclosed in the literature (J. Med. Chem., 1988, Vol.31, p.422), 10.2g of a crude compound containing the title compound was obtained in the form of a yellow solid from lOg of piperidin-2-one. This mixture was purified by silica gel chromatography, to give 2.0 g of the title compound as a yellow solid.
1H-NMR (400MHz, DMSO-D6) δ 1.67-1.74 (1H, m) , 1.84-1.95 (1H, m), 2.05-2.12 (1H, m), 2.23-2.31 (1H, m), 3.19 (2H, m) , 4.59 (1H, t, J=4.8Hz), 7.77 (1H, bs) Production Example 1306 3-Azido-piperidin-2-one
To a solution of 2.0 g of 3-bromopiperidin-2-one in 30 ml of N, N-dimethylformamide was added 5.9 g of sodium azide, followed by stirring at 60°C overnight. After cooling down to room temperature, water was added to the reaction mixture and then the mixture was extracted with ethyl acetate. The organic layer was washed with water and saturated brine, dried over anhydrous magnesium sulfate and the solvent was evaporated, to give 890 mg of the title compound as a yellow oil. 1H-NMR (400MHz, DMSO-D6) δ 1.49-1.58 (1H, m) , 1.60-1.75 (2H, m) , 1.93-2.00 (1H, m) , 3.09-3.12 (2H, m) , 4.13 (1H, dd, J=6.0, 9.6Hz), 7.82 (1H, bs) Production Example 1307 3-Amino-piperidin-2-one
To a solution of 890 mg of 3-azido-piperidin-2-one in 10 ml of ethanol was added 89 mg of 10% palladium on carbon, followed by stirring in a hydrogen atmosphere at room temperature overnight. The catalyst was filtered off through Celite and the filtrate was evaporated, to give 700 mg of the title compound as a yellow oil.
1H-NMR (400MHz, DMSO-D6) δ 1.38-1.48 (1H, m) , 1.58-1.83 (2H, m), 1.92-1.97 (1H, m), 2.50 (1H, t, J=3.6Hz), 3.08-3.12 (2H, m), 7.40 (1H, bs) Example 1308
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid obtained in Example 234 was condensed according to a similar method as in Example 44 with the amines
obtained in Production Examples 1303, 1304 or 1307, or with
commercially available amines, and then purified by LC-MS, to
give the compounds of Examples 1309 to 1316.
Example 1309
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (cyano-phenyl-methyl)-amide
MS (ESI) m/z 427 MH+
Example 1310
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid [cyano-(pyridin-2-yl)-methyl]-amide
MS (ESI) m/z 428 MH+
Example 1311
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid [(ethyl-methyl-carbamoyl)-methyl]-amide
MS (ESI) m/z 411 MH+
Example 1312
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-lH-indazol-5-
carboxylic acid [(isopropyl-methyl-carbamoyl)-methyl]-amide
MS (ESI) m/z 425 MH+
Example 1313
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid [(isobutyl-methyl-carbamoyl)-methyl]-amide
MS (ESI) m/z 439 MH+
Example 1314
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid [(cyanomethyl-methyl-carbamoyl)-methyl]-
amide
MS (ESI) m/z 422 MH+
Example 1315
3- [ (E) -2- (4-Fluorophenyl) -vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid [2-(azetidin-1-yl)-2-oxo-ethyl]-amide
MS (ESI) m/z 409 MH+
Example 1316
3- [ (E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid (2-oxo-piperidin-3-yl)-amide
MS (ESI) m/z 409 MH+
Example 1317
1-({3-[(E) -2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-
indazol-5-carbonyl}-amino)-cyclopropane carboxylic acid
methyl ester
536 mg of the title compound was obtained as a brown solid according to the method of Example 1207 from 400 mg of 3- [ (E) -2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid obtained in Example 234 and 233 mg of 1-aminocyclopropane carboxylic acid methyl ester hydrochloride.
1H-NMR (400MHz, DMSO-D6) δ 1.22 (2H, dd, J=4.0, 7.2Hz), 1.47 (2H, dd, J=4.8, 8.0), 3.64 (3H, s), 3.96 (3H, s), 7.25 (2H, t, J=8.8Hz), 7.31 (1H, d, J=8.8Hz), 7.51 (1H, d, J=16.4Hz), 7.52 (1H, d, J=8.4Hz), 7.57 (1H, d, J=16.4Hz), 7.70 (1H, dd, J=5.2, 8.8Hz), 8.83 (1H, s) Example 1318
1-({3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-carbonyl}-amino)-cyclopropane carboxylic acid
To a mixed solution of 200 mg of 1-({3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-carbonyl}-amino)-cyclopropane carboxylic acid methyl ester obtained in Example 1317 in 5 ml of tetrahydrofuran and 2 ml of methanol was added 2 ml of a 1N sodium hydroxide aqueous solution, followed by stirring at room temperature overnight. The reactionmixture was diluted with water and the aqueous layer was washed with diethyl ether. The aqueous layer was adjusted to pH 2 by adding 1N hydrochloric acid, and the precipitated solid was collected by filtration with glass filter. The solid was washed with water and was dried under reduced pressure, to give 151 mg of the title compound as a brown solid.
1H-NMR (400MHz, DMSO-D6) δ 1.17 (2H, dd, J=4 . 4, 7 . 6Hz) , 1.43 (2H, dd, J=4.8, 7.6), 3.96 (3H, s) , 7.25 (2H, t, J=8.8Hz), 7.30 (1H, d, J=8.4Hz), 7.51 (1H, d, J=16.8Hz), 7.54 (1H, d, J=8.8Hz), 7.56 (1H, d, J=16.4Hz), 7.70 (1H, dd, J=5.6,8.8Hz), 8.73 (1H, s) Example 1319
The compounds of Examples 1320 to 1332 were obtained according to the method of Example 102 from various amines and 1-({3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-carbonyl}-amino)-cyclopropane carboxylic acid obtained in Example 1318. Example 1320 3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (1-carbamoyl-cyclopropyl)-amide
MS (ESI) m/z 395 MH+
Example 1321
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (1-methylcarbamoyl-cyclopropyl)-amide
MS (ESI) m/z 409 MH+
Example 1322
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (1-dimethylcarbamoyl-cyclopropyl)-amide
MS (ESI) m/z 423 MH+
Example 1323
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-1H-indazol-5-
carboxylic acid [1-(ethyl-methyl-carbamoyl)-cyclopropyl]-
amide
MS (ESI) m/z 437 MH+
Example 1324
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid [1-(isopropyl-methylcarbamoyl)-cyclopropyl]-
amide
MS (ESI) m/z 451 MH+
Example 1325
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid [1-(isobutyl-methylcarbamoyl)-cyclopropyl]-
amide
MS (ESI) m/z 465 MH+
Example 1326
3-[(E)-2-(4-fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (1-diethylcarbamoyl-cyclopropyl)-amide
MS (ESI) m/z 451 MH+
Example 1327
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid {1-[(azetidin-1-yl)carbonyl]-cyclopropyl}-
amide
MS (ESI) m/z 435 MH+
Example 1328
3- [ (E) -2- (4-Fluorophenyl) -vinyl] -4-methoxy-lH-indazol-5-
carboxylic acid {1-[(pyrrolidin-1-yl)carbonyl]-cyclopropyl}-
amide
MS (ESI) m/z 449 MH+
Example 1329
3-[{E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid {1-[(piperidin-1-yl)carbonyl]-cyclopropyl}-
amide
MS (ESI) m/z 463 MH+
Example 1330
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid {1-[(morpholin-4-yl)carbonyl]-cyclopropyl}-
amide
MS (ESI) m/z 465 MH+
Example 1331
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (tra.ns-2-hydroxy-cyclopentyl) -amide
MS (ESI) m/z 396 MH+
Example 1332
3-[(E)-2-(4-Fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (trans-2-hydroxy-cyclohexyl)-amide
MS (ESI) m/z 410 MH+
Example 1333
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-
methoxy-lH-indazol-5-carboxylic acid ethyl ester
590 mg of the 3-iodo-4-methoxy-l-trityl-lH-indazol-5-carboxylic acid ethyl ester obtained in Production Example 276 and 250 mg of 6-vinyl-2, 3-dihydrobenzo [1,4] dioxine were reacted according to a similar method as in Production Example 181, and then the product was deprotected according to a similar method as in Example 16, to give 150 mg of the title compound as a pale yellow oil.
1H-NMR (400MHz, CDCl3) δ 1.46 (3H, t, J=7.2Hz), 4.04 (3H, s), 4.30 (4H, s), 4.43 (2H, q, J=7.2Hz), 6.88 (1H, d, J=8.8Hz), 7.10 (1H, dd, J=2.0,8.8Hz), 7.13 (1H, d, J=2.0Hz), 7.20 (1H, d, J=8.8Hz), 7.51 (1H, d, J=16.4Hz), 7.55 (1H, d, J=16.4Hz), 7.90 (1H, d, J=8.8Hz). Example 1334
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid
150 mg of 3-[(E)-2-(2,3-dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-1H-indazol-5-carboxylic acid ethyl ester was treated according to a similar method as in Example 144, to give
140 mg of the title compound as a colorless powder.
1H-NMR (400MHz, DMSO-D6) δ 3.95 (3H, s) , 4.27 (4H, s), 6.90 (1H,
d, J=8.8Hz), 7.12 (1H, s), 7.13 (1H, dd, J=2.0, 8.8Hz), 7.29
(1H, d, J=8.8Hz), 7.40 (1H, d, J=16.4Hz), 7.44 (1H, d, J=16.4Hz),
7.73 (1H, d, J=8.8Hz), 13.39 (1H, bs).
Example 1335
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid ethyl ester
590 mg of 3-iodo-4-methoxy-l-trityl-lH-indazol-5-carboxylic acid ethyl ester obtained in Production Example 276 and 430 mg of crude 5-vinylbenzofuran were reacted according to a similar method as in Production Example 181, and then the product was deprotected according to a similar method as in Example 16, to give 125 mg of the title compound as a colorless oil.
1H-NMR (400MHz, CDCl3) δ 1.45 (3H, t, J=7.2Hz), 4.07 (3H, s) , 4.4 4 (2H, q, J=7.2Hz) , 6.80 (1H, d, J=2.0Hz) , 7.21 (1H, d, J=8.8Hz) , 7.52 (1H, d, J=8.4Hz), 7.59 (1H, dd, J=2.0, 8.4Hz), 7.64 (1H, d, J=2.0Hz), 7.66 (1H, d, J=16.6Hz), 7.76 (1H, d, J=16.6Hz), 7.80 (1H, d, J=2.0Hz), 7.90 (1H, d, J=8.8Hz). Example 1336
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid
125 mg of 3-[(E)-2-(benzofuran-5-yl)vinyl]-4-methoxy-lH-indazol-5-carboxylic acid ethyl ester was treated according to a similar method as in Example 144, to give 120 mg of the title
compound as a colorless powder.
1H-NMR (400MHz, DMSO-D6) δ 4.00 (3H, s) , 7.00 (1H, d, J=2.0Hz),
7.30 (1H, d, J=8.6Hz), 7.58 (1H, d, J=16.4Hz), 7.64 (1H, d,
J=8.3Hz), 7.65 (1H, d, J=8.3Hz), 7.67 (1H, d, J=16.4Hz), 7.74
(1H, d, J=8.6Hz), 7.12 (1H, bs), 8.02 (1H, d, J=2.0Hz), 13.41
(1H, bs) .
Example 1337
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-
indazol-5-carboxylic acid ethyl ester
580 mg of 3-iodo-4-methoxy-l-trityl-1H-indazol-5-carboxylic acid ethyl ester obtained in Production Example 276 and 210 mg of 1-(2-fluoro-5-vinylphenyl)ethanone were reacted according to a similar method as in Production Example 181, and then the product was deprotected according to a similar method as in Example 16, to give 120 mg of the title compound as a colorless powder.
1H-NMR (400MHz, CDCl3) δ 1.45 (3H, t, J=7.2Hz), 2.69 (3H, d, J=4.8Hz), 4.05 (3H, s), 4.44 (2H, q, J=7.2Hz), 7.17 (1H, dd, J=8.8, 10.8Hz), 7.23 (1H, d, J=8.8Hz), 7.63 (1H, d, J=16.4Hz), 7.65 (1H, d, J=16.4Hz), 7.70 (1H, ddd, J=2.4, 4.8, 8.8Hz), 7.91 (1H, d, J=8.8Hz), 8.08 (1H, dd, J=2.4, 7.2Hz). Example 1338
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-1H-indazol-5-carboxylic acid
120 mg of 3-[(E)-2-(3-acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid ethyl ester was treated
according to a similar method as in Example 144, to give 110 mg of the title compound as a colorless powder. 1H-NMR (400MHz, DMSO-D6) δ 2.63 (3H, d, J=4.0Hz), 3.96 (3H, s), 7.32 (1H, bd, J=8.8Hz), 7.41 (1H, dd, J=8.7, 10.6Hz), 7.59 (1H, d, J=16.4Hz), 7.62 (1H, d, J=16.4Hz), 7.75 (1H, bd, J=8.8Hz), 7.95-8.01 (1H, m), 8.02 (1H, bd, J=7.2Hz), 13.52 (1H, bs). Example 1339
The compounds of Examples 1340 to 1363 were obtained by condensation of the carboxylic acids obtained in Examples 1334, 1336 and 1338 with various amines f ol lowed by LC-MS purification, according to a similar method as in Example 44. Example 1340
3-[(E)-2-(2,3-Dihydrobenzo[l,4]dioxin-6-yl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid (pyrazin-2-ylmethyl)amide MS (ESI) m/z 444 MH+ Example 1341
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-1H-indazol-5-carboxylic acid (furan-2-ylmethyl)amide MS (ESI) m/z 432 MH+ Example 1342
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid cyclopropylmethylamide MS (ESI) m/z 406 MH+ Example 1343 3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-
methoxy-lH-indazol-5-carboxylic acid (thiophen-2-ylmethyl)amide MS (ESI) m/z 448 MH+ Example 1344
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-1H-indazol-5-carboxylic acid cyclopropylamide MS (ESI) m/z 392 MH+ Example 1345
3-[(E)-2-(2,3-Dihydrobenzo[1, 4]dioxin-6-yl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid (pyridin-3-ylmethyl)amide MS (ESI) m/z 443 MH+ Example 134 6
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-1H-indazol-5-carboxylic acid cyanomethylamide MS (ESI) m/z 391 MH+ Example 1347
3-[(E)-2-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-vinyl]-4-methoxy-lH-indazol-5-carboxylic acid (2-hydroxypropyl)amide MS (ESI) m/z 410 MH+ Example 1348
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-1H-indazol-5-carboxylic acid (pyrazin-2-ylmethyl)amide MS (ESI) m/z 446 MH+ Example 1349 3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-
indazol-5-carboxylic acid (furan-2-ylmethyl)amide
MS (ESI) m/z 434 MH+
Example 1350
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-
indazol-5-carboxylic acid cyclopropylmethylamide
MS (ESI) m/z 408 MH+
Example 1351
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-1H-
indazol-5-carboxylic acid (thiophen-2-ylmethyl)amide
MS (ESI) m/z 450 MH+
Example 1352
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-
indazol-5-carboxylic acid cyclopropylamide
MS (ESI) m/z 394 MH+
Example 1353
3-[(E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-
indazol-5-carboxylic acid (pyridin-3-ylmethyl)amide
MS (ESI) m/z 445 MH+
Example 1354
3-[{E)-2-(3-Acetyl-4-fluorophenyl)-vinyl]-4-methoxy-lH-
indazol-5-carboxylic acid cyanomethylamide
MS (ESI) m/z 393 MH+
Example 1355
3- [ (E) -2- (3-Acetyl-4-fluorophenyl) -vinyl] -4-methoxy-1H-
indazol-5-carboxylic acid (2-hydroxypropyl)amide
MS (ESI) m/z 412 MH+
Example 1356
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid (pyrazin-2-ylmethyl)amide
MS (ESI) m/z 426 MH+
Example 1357
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid (furan-2-ylmethyl)amide
MS (ESI) m/z 414 MH+
Example 1358
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-1H-indazol-5-
carboxylic acid cyclopropylmethylamide
MS (ESI) m/z 388 MH+
Example 1359
3- [ (E) -2- (Benzofuran-5-yl) -vinyl] -4-methoxy-1H-indazol-5-
carboxylic acid (thiophen-2-ylmethyl)amide
MS (ESI) m/z 430 MH+
Example 1360
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid cyclopropylamide
MS (ESI) m/z 374 MH+
Example 1361
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (pyridin-3-ylmethyl)amide
MS (ESI) m/z 425 MH+
Example 1362
3-[{E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid cyanomethylamide
MS (ESI) m/z 373 MH+
Example 1363
3-[(E)-2-(Benzofuran-5-yl)-vinyl]-4-methoxy-lH-indazol-5-
carboxylic acid (2-hydroxypropyl)amide
MS (ESI) m/z 392 MH+
The compounds (I) to (III) according to the present invention or a salt thereof exhibited an excellent action in tests for determining JNK inhibitory action. For example, the inhibitory actions on JNK 3 were as follows. Test Example 1 Measurement of JNK 3 Inhibition
Human JNK 3 was expressed as a fusion protein (GST-JNK 3) with glutathione S-transferase (GST) in Escherichia coli and was purified using glutathione Sepharose 4B beads. The amino acid sequence 1-169 of c-Jun was prepared as a fusion protein (GST-c-Jun) with GST in Escherichia coli, was purified using glutathione Sepharose 4B beads and was used as a substrate. A test compound was solved in 100% dimethyl sulfoxide into 10 mM and was then further diluted with 10% aqueous dimethyl sulfoxide solution to yield a dilution series. In 96-well OPTI plate (available from ParkinElmer Life Sciences), 20 ul of diluted compound, 30 ul of substrate solution (1.2 pg GST-c-Jun, 0.2 uCi [y-33P]ATP, 25 mM HEPES pH=7.5, 10 mM MgAcetate, 3.33 uM ATP) and 50 ul of enzyme solution (0.04 ug GST-JNK3, 25 mM HEPES pH=7.5, 10 mM Mg Acetate) were mixed per 1-well, made up to 100
µl, and allowed to react for 30 minutes. After terminating the reaction by adding 100 yl of a reaction terminator (80 mM ATP, 50mg/ml glutathione SPAbeads (available f romAmersham Pharmacia Biotech) ) , the reaction mixture was shaken for 30 minutes. The mixture was centrifuged at room temperature at 1000 x g for 5 minutes, and the emission intensity thereof was determined on a TopCount™ illuminator (available from available from ParkinElmer Life Sciences). The activity is expressed by the 50% inhibitory concentration on the enzymatic activity of JNK, i.e., IC50 (nM) . Results
The compounds (I) to (III) according to the present invention or a salt thereof exhibited an excellent JNK3 inhibitory action.
The following shows examples of IC50 values.
Example No. JNK3 inhibitory Example No. JNK3 inhibitory
(Table Removed)
Test example 2
Measurement of ERK2 inhibition
A plate for ELISA was coated for 1 hour with 1 yg/well myelin basic protein (available from Upstate Biotech) serving as a
reaction substrate. Following washing operation with PBS-Tween, blocking with 1% BSA-PBS was conducted for 3 hours . After washing with PBS-Tween, 25 pi of a reaction solution (20 mM MOPS, 25 mM [ß-glycerophosphoric acid, 5 mM EGTA, 1 mM orthovanadic acid, 1 mM dithiothreitol) , 5 ul of a dilution sample of compound from dilution series which was prepared using 1% DMSO-1% BSA solution, 10 ul of 0.5 pg/ml ERK2(available from Upstate Bioech), 10 pi of 500 pM ATP were added to made up to 50 ul, and allowed to react at room temperature for 30 minutes. 100 µl of 200 mM EDTA (pH 8.0) was added to stop the reaction, which was then washed with PBS-Tween. This was then added with mouse anti-phospho-myelin basic protein antibody (available from Usptate Biotech), allowed to react for 1 hour, and washed with PBS-Tween, thereafter added with anti-mouse IgG antibody combined with horseradish peroxidase (available from ICN), allowed to react for 1 hour, and washed with PBS-Tween. Subsequently 100 µl of a TMB peroxidase substrate solution (available from KPL) was added, coloring reaction was allowed for 10 minutes, and then measurement at OD 450nm was conducted. The activity is represented by the concentration at which ERK2 enzyme activity was inhibited to 50%, namely by IC50(nM). Results
The compounds of the present invention exhibited an excellent selectivity.
(Table Removed)

The compound of Reference example 1, 3-[(E)-2-(3-fluorophenyl)-vinyl]-lH-indazole-5-carboxylic acid (furan-2-ylmethyl) -amide was synthesized while referring to WO02-10137(A2). MS (ESI) m/z 362 MH+
The compounds according to the present invention had an excellent JNK3 inhibitory action as described above, and hence desired results were obtained also in the following tests. Test example 3
Cell death induced by low K+ exposure in primary culture of mouse cerebellar granular neurons
Cerebellum was removed from an ICR mouse (CHARLES RIVER JAPAN, INC.) of 7-8 days old, and cerebellar granular neurons were isolated by means of enzyme treatment and physical treatment. The cerebellar granular neurons were seeded in a 96-well plate so that the density was 3xl05-4xl05 cells/cm2, and cultured for about 1 week in a culture medium A (Basal medium Eagle + 10% FBS, 20 mM K+) . Then the medium was changed from the culture medium A to a culture medium B in which K+ concentration was low, namely Basal medium Eagle. A test compound was dissolved in 100% DMSO to concentration of 10 mM, diluted with the culture medium B into an objective concentration, and added at the time
of medium replacement. After 24 hours from medium replacement,
survival of cerebellar granular cells was determined by MTT
assay.
Test example 4
Cell death induced by MPP+ in primary culture of rat mesencephalic
dopaminergic neurons
Ventral mesencephalon was removed from 14-day fetal Wistar rat (CHARLES RIVER JAPAN, INC.), and mesencephalic neurons were isolated by means of enzyme treatment and physical treatment. The mesencephalic neurons were seeded in a 48-well or 96-well plate so that the density was 2xl05 cells/cm2, and cultured for about 1 week in a culture medium (DMEM+ 10% FBS) . To this medium, a solution of test compound dissolved in 100% DMSO to 10 mM and diluted with the culture medium into x22 concentration of objective concentration was added in an amount of 1/22 of the medium volume, and next 660 uM MPP+ was added in an amount of 1/22 of the medium volume. After 48 hours from addition of MPP+, the cells were fixed by paraformaldehyde, andimmunostainedusing anti-tyrosine hydroxylase antibody. Then surviving cells was counted under microscope, and quantified by using an image analyzer (ANAX50, Kyusyu Matsushita Electric Co., Ltd.) Test example 5
Cell death induced by Amyloid (3 (Aß) in primary culture of rat cerebral cortex and hippocampus neurons
Cerebral cortex and hippocampus were removed from 17-day fetal Wistar rat (CHARLES RIVER JAPAN, INC.), and neurons of
each site were isolated by means of enzyme treatment and physical treatment. Each neurons were seeded in a 96-well plate so that the density was 2x105 cells/cm2, and cultured for 4 days in a culture medium A (Neurobasal™mediuM + B27 supplement) . To this medium, a solution of test compound dissolved in 100% DMSO to l0mM and diluted into an objective concentration with the culture medium B (D/F medium + N2 supplement) was added, and then an appropriate concentration of A(31-40 was added in an amount of 1/10 of the medium volume . After 4 8 hours from addition ofAß1-40, cell injury was quantitatively measured by LDH assay. Test example 6
Protective effect against dopaminergic neuron degeneration in MPTP-treated mouse
To a group consisting of 5 male C57BL/6 mice (Japane SLC) in 8-10 weeks old, 40 mg/kg of MPTP hydrochloride (Sigma) dissolved in saline was subcutaneously administered. After 3 days, contents of dopamine and its metabolites (DOPAC and HVA) in striatum were measured by HPLC. For determination of drug effect, a drug was orally or intrapenetorially administered twice a day including 1 hour before treating of MPTP. The recovery rate of dopamine content owing to administration of drug was determined in accordance with the following formula.
Recovery rate (%)=100X[(striatal dopamine content in MPTP-untreated mouse)-(striatal dopamine content of drug-administered group in MPTP-treated mouse)/(striatal dopamine content in MPTP-untreated mouse)-(striatal dopamine
content of control group in MPTP-treated mouse)]
Test example 7
LPS induced tumor necrosis factor a (TNF-α) production
A group consisting of 5 male C57BL/6 mice (Japane SLC) in 7-12 weeks old was sensitized by intravenously administering 2 mg of BCG vaccine (Japan BCG) . After 1 to 2 weeks, 0.3 mg/kg of lipopolysaccharoid (LPS; Sigma) was intravenously administered, and after 1 hour plasma was obtained by collecting blood from cerebral hemorrhage. TNF-a produced in plasma was measuredby usinga commercially available ELISA kit (Biosource) . For determination of drug effect, a drug was orally or intrapenetorially administered 1 hour before treating of LPS. The suppression rate of TNF-a production owing to drug was determined in accordance with the following formula.
Suppression rate (%)=100[1-(plasma TNF-a concentration of drug-administered group)/(plasma TNF-a concentration of control group)] Test example 8
Analysis using insulin-resistant model mouse (db/db mouse, ob/ob mouse)
A db/db mouse or an ob/ob mouse was administered with a test compound once a day for 7 days, and blood glucose was measured in fasting state and non-fasting state. Also after single administration and final administration, glucose tolerance test with 2g glucose/kg was conducted. Furthermore, organs were extracted during autopsy, and a variety of phosphorylation of
c-jun or IRS-1 in different organs were analyzed.
Structural formulae for compounds of the above Production examples and Examples are shown below.
(Table Removed)






We Claim:
1. An indazol compound JNK inhibitor represented by the formula (III), a salt thereof or a hydrate of them
(Formula Removed)
wherein
R1 designates a group represented by the formula -CRb=CRc-Ar wherein Rb and Rc each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group; Ar designates an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group;
Rd and Rf each designates a hydrogen atom and Re designates a halogen atom, hydroxyl group, cyano group, nitro group, carboxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-7 acyl group, -CO-NR2aR2b, -NR2bCO-R2c or -NR2aR2b (wherein R2a and R2b each independently designate a hydrogen atom or an optionally substituted C1-6 alkyl group);
L designates a single bond, an optionally substituted C1-6 alkylene group, an optionally substituted C2-6 alkenylene group or an optionally substituted C2-6 alkynylene group;
X designates a single bond, or a group represented by -NR7-, -O-, -CO-, -S-, -SO-, -SO2-, -CO-NR8-Z-, -C(O)O-, -NR8-CO-Z-, -NR8-C(O)O-, -NR8-S-, -NR8-SO-, -NR8-SO2-Z-, -NR9-CO-NR10-, -NR9-CS-NR10-, -S(O)m-NRn-Z-, -C(=NR12)-NR13-, -OC(O)-, -OC(O)-NR14- or -CH2-NR8-COR7- (wherein R7, R8, R9, R10, R11, R12, R13 and R14 each independently designate a hydrogen atom, halogen atom, hydroxyl group, an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C2-6 alkenyloxy group, an optionally substituted C1-6 alkylthio group, an optionally substituted C2-6 alkenylthio group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group, Z designates a single bond or an optionally substituted C1-6 alkylene group, and m designates 0, 1 or 2); and
Y designates any one group selected from the group consisting of a halogen atom, nitro group, hydroxyl group, cyano group, carboxyl group or an optionally substituted C1-6 alkyl group, an optionally substituted C2-6 alkenyl group, an optionally substituted C2-6 alkynyl group, an optionally substituted C1-6 alkoxy group, an optionally substituted C3-8 cycloalkyl group, an optionally substituted C3-8 cycloalkenyl group, an optionally substituted 4- to 14-membered non-aromatic heterocyclic group, an optionally substituted C6-14 aryl group, an optionally substituted 5- to 14-membered heteroaryl group, an optionally substituted amino group and a group represented by the formula -W-R15 (wherein W designates CO or SO2; and R15 designates an optionally substituted C1-6 alkyl group, an optionally substituted amino group, an optionally substituted C6-14 aryl group or an optionally substituted 5- to 14-membered heteroaryl group).
2. The compound as claimed in claim 1, a salt thereof or a hydrate of them, wherein Re is a halogen atom or an optionally substituted C1-6 alkoxy group.
3.. The compound as claimed in claim 1, a salt thereof or a hydrate thereof, wherein
L and X are a single bond, and
Y is 5- to 6-membered heteroaryl group, and Y is optionally substituted with 1 to 3 group(s) selected from the group consisting of
(1) (a) C1-6 alkyl groups, (b) C1-6 alkenyl groups, (c) C1-6 alkynyl groups, (d) C1-6 alkoxy groups, (e) C2-7 acyl groups, (f) amide group, (g) amino group, (h) C3-8 cycloalkyl groups, (i) C3-8 cycloalkenyl groups, (1) C6-14 aryl groups, (k) 5- to 14-membered heteroaryl groups, (1) C6-14 aryloxy groups, and (m) 4- to 14-membered non-aromatic heterocyclic groups; each optionally substituted,
(2) halogen atom,
(3) hydroxyl group,
(4) nitro group,
(5) cyano group, and
(6) carboxyl group,
4. A pharmaceutical composition as and when prepared by using the compound as claimed in claim 1, a salt thereof or a hydrate thereof.


Documents:

2918-delnp-2004-abstract.pdf

2918-delnp-2004-assignment.pdf

2918-delnp-2004-claims.pdf

2918-delnp-2004-complete specification (as filed).pdf

2918-delnp-2004-complete specification (granted).pdf

2918-delnp-2004-correspondence-others.pdf

2918-delnp-2004-correspondence-po.pdf

2918-delnp-2004-description (complete).pdf

2918-delnp-2004-form-1.pdf

2918-delnp-2004-form-18.pdf

2918-delnp-2004-form-2.pdf

2918-delnp-2004-form-3.pdf

2918-delnp-2004-form-5.pdf

2918-delnp-2004-form-6.pdf

2918-delnp-2004-gpa.pdf

2918-delnp-2004-pct-210.pdf

2918-delnp-2004-pct-304.pdf

2918-delnp-2004-pct-306.pdf

2918-delnp-2004-pct-308.pdf

2918-delnp-2004-pct-332.pdf

2918-delnp-2004-petition-137.pdf

2918-delnp-2004-petition-138.pdf

abstract.jpg


Patent Number 245616
Indian Patent Application Number 2918/DELNP/2004
PG Journal Number 04/2011
Publication Date 28-Jan-2011
Grant Date 27-Jan-2011
Date of Filing 28-Mar-2004
Name of Patentee EISAI R&D MANAGEMENT CO., LTD
Applicant Address 6-10, KOISHIKAWA 4-CHOME, BUNKYO-KU, KOKYO, JAPAN.
Inventors:
# Inventor's Name Inventor's Address
1 NORIHITO OHI 1-12-7, NAPEIDAI, AMIMACHI, INASHIKI-GUN, IBARAKI, JAPAN.
2 NOBUAKI SATO 1032-19, OHAZA OTTO, TSUCHIURA-SHI, IBARAKI, JAPAN.
3 MOTOHIRO SOEJIMA ESPERA DOMO 1-102, 4-8-7, AMAKUBO, TSUKUBA-SHI, IBARAKI, JAPAN.
4 TAKASHI DOKO ERANDO TSUKUBANEDAINIRYO 204, 9-7, INARIMAE, TSUKUBA-SHI, IBARAKI, JAPAN.
5 TARO TERAUCHI PAINHAITSU 201, 3-17-17, MATSUSHIRO, TSUKUBA-SHI, IBARAKI, JAPAN.
6 YOSHIMITSU NAOE 2574-20-B102, KAMIYOKOBA, TSUKUBA-SHI, IBARAKI, JAPAN.
7 TAKAFUMI MOTOKI MEZON GAKUEN 301, 2-23-5, AMAKUBO, TSUKUBA-SHI, IBARAKI, JAPAN.
PCT International Classification Number C07D 231/56
PCT International Application Number PCT/JP03/06777
PCT International Filing date 2003-05-29
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 2003-153 2003-01-06 Japan
2 2002-158467 2002-05-31 Japan