Title of Invention

"A PROCESS FOR THE PREPARATION OF NEW SALTS OF HMG-COA REDUCTASE INHIBITOR"

Abstract The invention describes new amine salts of HMG -CoA Reductase Inhibitors, their preparation and their use for purification. Further, the amine salts of the invention are used in the process for semisynthetic preparation, for biotechnological modification as well as for conversion into the pharmaceutically acceptable salts or the lactone form of the HMG-CoA reductase inhibitors.
Full Text Technical Field
Lovastatin, pravastatin, simvastatin, mevastatin, atorva-statin and derivatives and analogs thereof are examples of known as HMG-CoA reductase inhibitors which are used as antihypercholesterolemic agents. The majority of them are produced biotechnologically by fermentation using microorganisms of different species identified as species belonging to Aspergillus, Monascus, Nocardia, Amy cola tops is, Mucor or Penicillium genus, some are obtained by treating the fermentation products using the methods of chemical synthesis, thus leading to semisynthetic substances, or they are the products of total chemical synthesis.
The present invention relates to a new industrial process for isolation and/or purification of HMG-CoA reductase inhibitors via salts thereof with specific amines. The invention enables to obtain the pure amine salts of HMG-CoA reductase inhibitors from the fermentation broth in case the substances are produced by biotechnological (microbiological) processes, or from the reaction mixture in case the substances are produced by semisynthetic or total chemical synthesis. The step of forming salts with amine may be one of the steps in the process for isolation and/or purification of HMG-CoA reductase inhibitors or precursor substances thereof. The amines described in the present specification are very useful for the formation of salts in the composition of media in processes for biotechnological modification of HMG-CoA reductase
inhibitors or precursors thereof. The salts thus formed may be used as the starting substances or intermediates for the preparation of semisynthetic derivatives and analogs thereof, or by employing simple techniques known from the literature, if required, to be converted into the pharmaceutically acceptable salts and lactones, respectively.
Prior Art
The processes for the isolation and purification of antihypercholesterolemic agents known from patent and technical literature include different combinations of extraction, chromatography, lactonisation and crystallisation methods. Some of them additionally include the isolation and purification via different salts. In US Pat. Nos. 4,342,767 and 4,319,039, the ammonium salt of lova-statin (in the carboxylate form) is isolated directly from the organic phase which has been extracted from the fermentation medium. In the same patent the preparation of ethylenediamine, tetramethylammonium, potassium and N-methylglucamine salts as well as the salts of different amino acids such as L-lysine, L-arginine and L-ornithine is also described. The aforementioned salts are prepared from the already purified substance and the option for their use in the process of isolation or purification is not mentioned. GB 2055100 A also describes the formation of the sodium and calcium salts of lovastatin, which comprises the extraction in methanol, two steps of preparative liquid reverse-phase chromatography, crystallisation from methanol and recrystallisation from ethanol, and the conversion into the salt using an aqueous solution of sodium or calcium hydroxide. However, without including various chromatography methods, the methods de-
scribed do not yield a product of the purity comparable to the product obtained by using the present invention. US Pat. No. 4,346,227 discloses a process for the preparation of the sodium salt of pravastatin wherein chromatographic techniques are also used but the final product is obtained only after lyophilisation which is not an economical process in a large scale production operations. EP 65,835 discloses the preparation of the L-ornithine and t-octyl-amine salts of tetrahydro-M-4 or tetrahydro-IsoM-4 (wherein M4 denotes a specific HMG-CoA reductase inhibitor, M-4 and IsoM-4 representing the isomers hydroxylated at 6- and 3-biphenyl ring position, respectively, and "tetrahydro" means that the condensed biphenyl ring system is fully hydrogenated) as final products, that is from the respectively purified sodium salts thereof, but not as intermediates via which the isolation would be carried out. Other salts of tetrahydro- M-4 or IsoM-4 with ammonia, an amino acid or an organic amine are also contemplated as final products, including octylamine, 2-ethylhexylamine, benzylamine, α-methyl-benzylamine, phenethylamine, dibenzylamine, N-methylbenzylamine, N,N-dimethylbenzylaminev N,N-diethylbenzylamine, N-ethyl-N-methylbenzylamine, tribenzylamine, cyclopentylamine, cyclohexylamine cycloheptylamine, N-methylcyclopentyl-amine, N-ethylcyclohexylamine, N-ethylcycloheptylamine, dicyclohexylamine, N,N-dimethylcyclopentylamine, N,N-dimethylcyclohexylamine, N,N-diethylcycloheptylamine, pyrrolidine, N-methylpyrrolidine, piperidine, N-methyl-piperidine and morpholine. GB 2073199 A also discloses the preparation of different salts of HMG-CoA reductase inhibitors from the already isolated substance in the lactone form. US Pat. Nos. 5,763,653 and 5,763,646 disclose the preparation of the cyclopropylamine and n-butylamine amides of lovastatin and their use in a process
of chemical semisynthesis of simvastatin. US Pat. No. 5,403,860 discloses, as final products, amine salts of octahydronaphthalene oxime derivatives of HMG-CoA inhibitors, the derivatives deriving from ML-236A, ML-236B, MB-530A and MB-530B. As final amine salts, t-octylamine, dibenzylamine, dicyclohexylamine, morpholine, D-phenylglycine alkylester and D-glucosamine salts are mentioned.
Technical Problem
In industry there exists a constant need for rationalisation of the production and shortening of the production processes as well as for the use of least expensive starting raw materials or intermediate substances. To date the isolation of the final products in the case of HMG-CoA reductase inhibitors has been a multi-stage process wherein each step adds its share to the losses resulting in the final yield rarely greater than 60%. In addition, a product in the lactone form or lactone converted into the sodium salt is used as the starting substance in the process of semisynthesis (e.g. in a process for preparing simvastatin) or biochemical conversion (e.g. in a process for preparing pravastatin). The preparation of lactone is one of the. least economical steps in the production of HMG-C-A reductase inhibitors since losses in the course of the conversion from the acid into the lactone form and optionally further into the salts are greater than 20%. Therefore, there is a constant need for the starting substances and/or the intermediate substances which would be sufficiently pure, with small losses during their conversion, low costs, and the preparation per se should be technologically simple.
Description of the Invention
In our developmental and research work we have surprisingly found that HMG-CoA reductase inhibitors form the salts with certain amines which crystallise from mother liquor once they are formed. It has surprisingly been found that crystals of the amine salt of the desired HMG-CoA reductase inhibitor of high purity may be obtained from the liquors containing a large number of impurities and undesired HMG-CoA reductase inhibitor analogs. Contrary to the statements from US Pat. No. 5,403,860 that lower yields are obtained when using the salts of HMG-CoA reductase inhibitor as starting or intermediate substances in a process for preparing the substances (Ia) mentioned below, we have surprisingly found that, when using the amine salts of HMG-CoA reductase inhibitors according to the present invention, the yields and the purity of the prepared HMG-CoA reductase inhibitors are equal to or greater than when using the,HMG-CoA reductase inhibitors in the lactone form.
(Formula Removed)
Furthermore, we surprisingly discovered that in processes for the biotechnological modification of HMG-CoA reductase inhibitors the formation of amine salts of HMG-CoA reductase inhibitors in the medium which derives from the fermentation liquor provides, in comparison with the mere metal salts as described in publicly accessible literature, an efficient means for the isolation and/or purification of HMG-CoA reductase inhibitors by means of simple crystallisation. The amines which are described in the present specification and which readily form salts with HMG-CoA reductase inhibitors are thus particularly suitable as auxiliary materials or processing aids for the isolation and/or purification of HMG-CoA reductase inhibitors. Furthermore, they can be excellently used as starting materials or intermediates of semisynthetic preparation or biotechnological modification of HMG-CoA reductase inhibitors and, furthermore, for the conversion into pharmaceutically acceptable salts or into the lactone form of the respective HMG-CoA reductase inhibitors. Accordingly, the novel amine salts of HMG-CoA reductase inhibitors of the present invention are also highly valuable as such.
The present invention provides:
a) the novel salts of HMG-CoA reductase inhibitors with organic amines as specified in claim 1, wherein those specific salts are excluded which are disclosed in the prior art, but in different contexts as mentioned above,
b) a process for the preparation of salts of HMG-CoA reductase inhibitors with amines as specified in claim 5,
c) a use of salts of HMG-CoA reductase inhibitors with amines as processing aids or starting substances or intermediate substances in various processes as specified in claim 18,
d) a process for the preparation of the pure HMG-CoA
reductase inhibitors from/via amine salts thereof, as
specified in claim 27,
e) a process for the semisynthetic preparation of HMG-CoA
reductase inhibitors, wherein the amine salts of HMG-CoA
reductase inhibitors are used as the starting
substances, as specified in claim 30,
f) a process for the biotechnological modification of HMG-
CoA reductase inhibitors, wherein one of the components
of the medium is the amine salt of HMG-CoA reductase
inhibitors, as specified in claim 33,
g) a process for the conversion of the amine salts of HMG-
CoA reductase inhibitors into the pharmaceutically
acceptable salts of HMG-CoA reductase inhibitors, as
specified in claim 37, and
h) a process for the conversion of the amine salts of HMG-CoA reductase inhibitors into HMG-CoA reductase inhibitors in the lactone form, as specified in claim 41.
The amine which is used according to the present invention for the formation of the salts with a HMG-CoA reductase inhibitor is selected from the group consisting of organic amines of the following formulae I and II:
(Formula Removed)
wherein:
al) R2, R2, R3 and R4 independently denote:
• a hydrogen atom
• a straight or a branched alkyl group having 1 to 8
carbon atoms, or
• a cycloalkyl group having 3 to 8 carbon atoms, or
• an arylalkyl group wherein the alkyl group is methyl or ethyl and the aryl group is phenyl, which is optionally substituted by an N-alkyl or N,N-dialkyl group wherein the alkyl group is alkyl having 1 to 4 carbon atoms, or
• an arylalkyl group which is optionally substituted by
one or more substituents,
• a hydroxyalkyl group having 2 to 4 carbon atoms, or
• an aminoalkyl group having 2 to 4 carbon atoms, which
are optionally substituted by an N-alkyl or N,N-dialkyl
group wherein the alkyl group is alkyl having 1 to 4
carbon atoms;
X denotes a hydrogen atom, a hydroxyl group, a halogen or a methyl group;
m and n independently denote an integer from 0 to 5; or
a2) NR1R2 or NR3R4 denote a heterocyclic ring having 3 to 7 methylene groups, one of these groups being optionally substituted by an oxygen or a sulphur atom or an imine group; X, m and n are as defined above;
(Formula Removed)
wherein:
bl) R'1, R'2' and R'3 are the same or different and denote hydrogen, alkyl, alkenyl, amino- or hydroxy- or alkoxy-substituted alkyl or alkenyl, or substituted amino-substituted alkyl or alkenyl, provided that R'1, R'2' and R'3 are not hydrogen at the same time; or
b2) R'1 and R'2' and optionally R'3, together with the nitrogen atom form an optionally substituted heterocyclic ring system including the nitrogen atom as a ring member, and optionally including an additional hetero atom, and if R'3 is not part of the ring system it is independently selected from hydrogen, alkyl, alkenyl, amino- or hydroxy-or alkoxy-substituted alkyl, or substituted amino-substituted alkyl; or
b3) R'1 is an optionally substituted cyclic group of general formula III,
(Formula Removed)
wherein m is zero or an integer from 1 to 5, R' is optionally substituted aliphatic hydrocarbon cyclic system having 3 to 8 carbon atoms in the ring, R'4 is hydrogen, or alkyl, amino- or hydroxy- or alkoxy- substituted alkyl, or substituted amino- substituted alkyl, or a group of the same general formula as R'1 as defined herein above; R'2 and R'3 are the same as R'1 or hydrogen, alkyl, alkenyl, amino- or hydroxy- or alkoxy- substituted alkyl, or substituted amino- substituted alkyl or alkenyl; or
b4) R' i is an optionally substituted aryl group of general formula IV:
(Formula Removed)
wherein R'5 is hydrogen or one or more substituents, and m is zero or an integer from 1 to 5; and R'2 and R'3 may be independently hydrogen, alkyl, amino- or hydroxy- or
alkoxy- substituted alkyl, or substituted amino-substituted alkyl, or groups of the same general formula
Substitutions which are not explicitly specified are usual "inert" substituents, such as halogens, a hydroxyl group, alkyl having 1 to 4 carbon atoms, alkoxyl having 1 to 4 carbon atoms, acyloxyl having 1 to 4 carbon atoms and esterified carboxyl having 1 to 4 carbon atoms.
Advantageous examples of amines which form the salts with HMG-CoA reductase inhibitors are: (±)-1,2-dimethylpropyl-amine, 3-(2-aminoethylamino)-propylamine, n-butylamine, secondary butylamine, tertiary butylamine (TBA), dibutyl-amine, tertiary amylamine, cyclopentylamine, cyclohexyl-amine, cycloheptylamine, dicyclohexylamine (DCHA), N-methylcyclohexylamine , N,N'-diisopropylethylenediamine (DIPEDA), N,N'-diethylenediamine, N-methyl-1,3-propanediamine, N-methylethylenediamine, N,N,N',N'-tetramethyl-1,2-diaminoethane, N,N,N',N'-tetramethyl-1, 4-diaminobutane, N,N,N',N'-tetramethyl-1,6-diaminohexane, 1,2-dipiperidinethane, dipiperidinemethane, 2-amino-3,3-dimethylbutane, N,N-dimethylcyclohexylamine, neopentylamine, adamantylamine, N,N-diethylcycohexylamine, N-isopropylcyclohexylamine, N-methylcyclohexylamine, cyclobutylamine and norborylamine. Preferably in terms of crystallisatipn efficiency, combined with low toxicity and low costs, the amine is selected from the group consisting of n-butylamine, secondary butylamine, TBA, dibutylamine, tertiary amylamine, cyclohexylamine, DCHA, N-methylcyclohexylamine and DIPEDA. The amine may particularly be selected from the group consisting of TBA, DIPEDA, DCHA and N-methylcyclohexylamine.
The amines specified above are advantageous over the direct isolation via the salts with ammonia in terms of purification efficiency- Furthermore, amines having a larger organic group, and especially those having bulky groups, generally show a more readily crystallisation and to a lower extend form salts with unwanted side products when compared with amines having small organic groups. Accordingly, amines having at least one hydrocarbon residue with secondary or tertiary carbon atoms, or with cyclic hydrocarbon structure (either aromatic or aliphatic), and organic diamines are particularly suitable for the present invention.
Any type of HMG-CoA reductase inhibitors can be used according to the present invention. Those HMG-CoA reductase inhibitors selected from the group consisting of mevastatin, pravastatin, lovastatin, simvastatin, fluvastatin and atorvastatin have shown good results and are particularly preferred.
For isolating and purifying the desired HMG-CoA reductase inhibitor, the amine salt is most effectively formed directly from the crude medium of the respective HMG-CoA reductase inhibitor, which crude medium is usually derived from a fermentation broth as the result of a biotechno-logical process or from a reaction mixture as the result of a semisythesis or the total synthesis and usually contains the desired HMG-CoA reductase inhibitor together with unwanted side products and impurities. The crude medium may preferably contain the HMG-CoA reductase inhibitor in its acid from, and the formation of the amine salt may be effected by simply adding the amine to the crude medium. The crude medium may be an organic phase or a mixture of an organic or an aqueous phase where the
impure HMG-CoA reductase inhibitor is present in an organic solvent, such as ethyl acetate, ether or acetonitrile. Ethyl acetate is preferred as the organic solvent. After the biotechnological treatment, the crude medium in the organic phase is preferably obtained from the fermentation broth by a process including the step of extracting the HMG-CoA reductase inhibitor into the aforementioned organic solvent.
The process for the preparation of the amine salts of HMG-CoA reductase inhibitors may include the following steps:
a) contacting the medium containing the HMG-CoA reductase inhibitor, which is preferably in the acid form in an organic solvent, with at least one of the amines specified above,
b) optionally: forming crystallisation nuclei by known techniques,
c) filtering the crystals crystallised out
d) washing the crystals with an organic solvent, and
e) drying the crystals
The term "contacting" includes the known techniques for the preparation of the salts from substances with acid properties and substances with alkaline properties. The crystallisation is preferably carried out at a temperature
between 0 and 30°C, more preferably between 4 and 22°C.
The term "organic solvent" means organic solvents which are used in industry such as ethyl acetate, butyl acetate, ether and acetonitrile, including their aqueous mixtures.
Since the amines specified above effectively form salts with the HMG-CoA reductase inhibitors, they are also particularly suitable as auxiliary materials or processing
aids in a process for preparing the HMG-CoA reductase inhibitor in a purified form. Previously isolated HMG-CoA reductase inhibitors can be thus obtained in a higher purity. The purified form is usually prepared by crystallisation. Accordingly, the present invention provides a process for the isolation and/or purification of a HMG-CoA reductase inhibitor.
In further aspects of the present invention, the salt as specified above is suitably used as the starting substance or the intermediate substance in a process for preparing the HMG-CoA reductase inhibitor which is in a modified form, in a pharmaceutically active salt form or in the lactone form. Specifically, the modified form is obtained by chemical modification or biotechnological modification, which modifications are known to those skilled in the art. The pharmaceutically active salt is preferably a metal salt, such as the sodium salt or the calcium salt.
In this connection, the term "a process for the semisynthetic preparation of HMG-CoA reductase inhibitors" means the preparation of HMG-CoA reductase inhibitors using any of the known chemical modifications of the HMG-CoA reductase inhibitors. An example of such process is the semisynthesis of simvastatin from lovastatin as the starting substance. Most preferably, the TBA salt of lovastatin is used as the starting substance.
Furthermore, the term "a process for the biotechnological modification of HMG-CoA reductase inhibitors" means the preparation of HMG-CoA reductase inhibitors using microorganisms or enzymatic systems thereof for modifying HMG-CoA reductase inhibitors. An example of such process is the biotechnological conversion of mevastatin into pravastatin. The amine salt is preferably the TBA salt.
Furthermore, the term "a process for the conversion of the amine salts of HMG-CoA reductase inhibitors into the pharmaceutically acceptable salts of HMG-CoA reductase inhibitors" includes processes for the preparation of HMG-CoA reductase inhibitors by one of the known methods wherein the amine salts of HMG-CoA reductase inhibitors are used as the starting substance. Specific examples of converted salts are the sodium salts of pravastatin and fluvastatin and the calcium salt of atorvastatin.
Furthermore, the term "conversion of the amine salts of HMG-CoA reductase inhibitors into HMG-CoA reductase inhibitors in the lactone form" includes processes for the preparation of HMG-CoA reductase inhibitors in the lactone form by one of the known methods wherein the amine salts of HMG-CoA reductase inhibitors are used as the starting substance. Examples of HMG-CoA reductase inhibitors converted into the lactone form are lovastatin, mevastatin or simvastatin.
The present invention is illustrated but in no way limited by the following examples.
(Formula Removed)
Example 1
Preparation of free acid of mevastatin and conversion thereof into salt thereof with tertiary butylamine (TBA)
Mevastatin (200 g) was suspended in 30% volume/volume (v/v) aqueous acetonitrile solution (2500 ml), 3 equivalents of triethylamine were added and the mixture was heated to 80°C and stirred for 30 minutes. After the
completed reaction, acetonitrile was evaporated, the remaining solution was acidified to pH 4 with phosphoric acid and extracted into ethyl acetate (2 x 1000 ml). The pooled extracts were dried by the addition of 30 g of sodium sulphate, the desiccant was filtered off and the solution was concentrated (950 ml). TBA (1.5 equivalents) was added to the solution and crystallisation was carried out for 30 minutes at 8°C. The crystals formed were filtered and washed with ethyl acetate (2 x 100 ml) and subsequently dried at 40°C for 15 hours. The crystals obtained (the TBA salts of mevastatin 215 g) were white in colour with a HPLC purity of 96.8%. The yield of the hydrolysis and crystallisation was 91%.
Example 2
Preparation of the sodium salt of mevastatin from the TBA salt of mevastatin
The TBA salt of mevastatin (1 g), obtained by the process disclosed in example 1, was dissolved in 3 ml of ethanol (96% v/v) and sodium hydroxide (40 g/L of ethanol) was added. The resulting mixture was precipitated in the ethyl acetate (60 ml). After the crystallisation (30 min) at 8°C the crystals were filtered off, washed with ethyl acetate and dried. The product: crystals of the sodium salt of mevastatin (0.65 g) pale brown in colour with a HPLC purity of 98%.
Example 3
Isolation of the TBA salts of lovastatin from the fermentation broth
A fermentation broth (160 L) obtained by the fermentation with a microorganism Aspergillus terreus ATCC 20544 and having a lovastatin content of 1 g/L was transferred from the fermenter into the tank (400 L) and pH was adjusted to 10 with the addition of 1M aqueous sodium hydroxide
solution. After 10 minutes of vigorous stirring the pH of the broth was decreased to 9 by adding 1M sulphuric acid solution and the biomass was filtered off. The filtrate obtained was acidified to a pH value of 6.5 with 1M sulphuric acid solution and 160 L of ethyl acetate was added. The slurry was subsequently stirred for 20 minutes. The aqueous and ethyl acetate phases were separated by extraction centrifuge and the ethyl acetate extract was concentrated in a rotavapor to the volume of 14 L. The concentration of lovastatin in the form of free acid in concentrated ethyl acetate extract was 10.1 g/L. To the obtained lovastatin solution {HPLC purity 72.7%) in the form of free acid in ethyl acetate (800 ml) 1.05 equivalents of TBA were added. After crystals were crystallised out, they were filtered off, washed with ethyl acetate (2 x 50 ml) and dried in a vacuum oven at 35°C for 24 hours. The yield: 8.06 g of crystals of the TBA salt of lovastatin with a HPLC purity of 99.2%.
Example 4
Preparation of the salt of lovastatin with tertiary amylamine
Lovastatin (5 g) was treated according to the process for hydrolysis disclosed in example 1 and sodium salt thereof (4.8 g) was prepared and dissolved in 100 ml of water. pH was adjusted to 4 with phosphoric acid (10% aqueous solution) and the formed free acid of lovastatin was extracted from the water phase into ethyl acetate (2 x 100 ml). The pooled ethyl acetate extracts were dried with sodium sulphate, the solution was filtered off and concentrated to ca. 100 ml. To the solution, prepared in the above manner (ca. 100 ml), 1.05 equivalents of tertiary amylamine were added. After crystals were crystallised out, they were filtered off, washed with ethyl acetate (2 x 10 ml) and dried in a vacuum oven (35°C,
24 hours). The yield: 4.8 g of crystals of- the tertiary
Example 5
Preparation of pravastatin In the free acid form and conversion Into the (+) -1,2-Dimethylpropylamlne salt thereof
Pravastatin (11 g, HPLC purity 97.2%) in the form of Na salt was dissolved in water (100 ml) and pH was adjusted to 4 with phosphoric acid (10% aqueous solution) . The resulting free acid was extracted from the water phase into ethyl acetate (2 x 100 ml). The pooled ethyl acetate extracts were dried with sodium sulphate, the solution was filtered off and concentrated to a volume of ca. 100 ml.
To the concentrate (9 ml) thus obtained, 1.5 equivalents of (±)-1,2-dimethylpropylamine were added. After crystals were crystallised out, they were filtered off, washed with ethyl acetate (2 x 10 ml) and dried in a vacuum oven at 35°C. The yield: crystals yellow in colour with a HPLC purity of 98.28%.
Examples 6-13
The conversion of the free acid form of pravastatin into the amine salt form as described in example 5 was repeated with other amines.
The amines used and results are shown in the Table below:

(Table Removed)
* from an oil formed, crystals are crystallised out overnight at 4°C .
Example 14
Preparation of the salt of pravastatin with secondary-butylamine from the crude sodium salt of pravastatin
According to the process described in example 5, pravastatin in the free acid form was prepared from the sodium salt of pravastatin (HPLC purity 83.6%). Further the salt of pravastatin with secondary butylamine was prepared by the process described in example 5. White crystals with a HPLC purity of 97.3% were obtained.
Example 15
Preparation of the sodium salt of pravastatin from the TBA salt of pravastatin (1)
8.9 g of the TBA salt of pravastatin as obtained in example 12 were dissolved in 22 ml of ethanol (96%) and
subsequently precipitated in 450 ml of ethyl acetate.
was carried out at 8°C for 60 minutes, the
portions of etnyl acetate and dried at 40°C for
g of the recrystallised TBA salt of pravastatin, obtained in the above-described manner, was dissolved in 5 ml of ethanolic solution of 0.43N NaOH and precipitated in 60 ml of ethanol. After 30 minutes at 8°C, crystals formed were filtered off and dried. The formed crystals of the sodium salt of pravastatin (0.75 g) were dark yellow in colour.
Example 16
Preparation of the sodium salt of pravastatin from the TBA salt of pravastatin (2)
The process described in example 15 was repeated wherein 1 g of the recrystallised TBA salt of pravastatin was dissolved in 3 ml of water, instead of ethanolic solution of 0.43N NaOH, and subsequently an aqueous sodium carbonate solution (equivalent) was added. The obtained solution was diluted with 5 ml of ethanol and the resulting sodium salt was precipitated with ethyl acetate. After 30 minutes at 8°C, crystals formed were filtered off and dried. The formed crystals of the sodium salt of pravastatin (0.65 g) were yellow in colour.
Example 11
Preparation of pravastatin from the TBA salt of mevastatin
PREPARATION OF INOCULUM FOR PRODUCTION
The colonies of microorganism Amycolatopsis orientalis ATCC 19795 were transferred to a sterile potter and homogenised. The resulting colonies were transferred to agar slopes and incubated in the thermostat at 26° to 30°C for 7 to 14 days. During that time surfaces of agar slopes were overgrown by cultures of homogeneous, folded, smooth,
white to pale greyish-blue mycelium. Further, 10 ml of sterile water was poured onto the agar slopes, the culture was scraped off with the pipette and the contents transferred into the potter. A portion (0.5 to 1 ml) of' the resulting culture was then inoculated into the vegetative medium.
Agar medium for the preparation of agar slopes and petri plates:

(Table Removed)
No pH adjustment needed.
VEGETATIVE PHASE OF FERMENTATION
The inoculum grown on the slope at 26° to 30oC for 10 days and prepared according to the above-described method was inoculated in a 500-ml Erlenmeyer flask containing 50 ml of the vegetative medium. After 24 hours of shaking at 220 rpm at 28°C, the culture was transferred onto the fermentation medium.
Vegetative medium:


(Table Removed)
Tap water to 1000 ml
CONVERSION OF THE TBA SALTS OF MEVASTATIN INTO PRAVASTATIN
The contents of fifteen Erlenmeyer flasks with the culture, prepared according to the above-described method, were used to inoculate the fermenter (50 L) w.ith 30 L of fermentation medium. After 20-hour fermentation, the solution of the TBA salt of mevastatin and glucose (1200 g of gl.ucose and 70 g of TBA salt of mevastatin (assay of mevastatin: 80%) dissolved in 5 L of water) was continually added to the medium at the flow rate 2 ml/min. During the fermentation 70% oxygen saturation of the medium was maintained by stirring with the frequency between 300 and 600 rpm. Analyses of the concentration of pravastatin in the fermentation broth showed the total final concentration of prevastatin in the fermentation broth to be 690 g/kg of broth after 76 hours of fermentation at temperature between 24° and 30°C, indicating a 40% conversion of mevastatin into pravastain.
Fermentation medium:

(Table Removed)
All raw materials were dissolved in tap water, the pH. was then adjusted to 7.4.
Example 18
Preparation of TBA salt of simvastatin
Simvastatin (1.95 g) was suspended in 50 ml of 30% (v/v) acetonitrile, triethylamine (1 ml) was added and the solution was heated for 20 minutes at 70°C. After completion of reaction acetonitrile was evaporated from the solution, the remaining solution was acidified to pH 4 with phosphoric acid. Simvastatin in the acid form was extracted into ethyl acetate (2 x 50 ml) and the pooled extracts were dried with 2 g of sodium sulphate. The desiccant was filtered off and the ethyl acetate solution was concentrated to 20 ml. To the solution 1.05 equivalents of TBA were added and crystallisation was carried out for 1 hour at 8°C. The product was filtered off and dried at 40°C for 1 hour. The yield: 2 g of the TBA salt of simvastatin.
Example 19
Conversion of the TBA salt of simvastatin into simvastatin lactone
The TBA salt of simvastatin (1.6 g), obtained by the process disclosed in example 18, was dissolved in water (36 ml), the solution was then acidified to pH 3.7 with an aqueous phosphoric acid solution and simvastatin in the acid form was extracted into ethyl acetate (2 x 50 ml). The pooled extracts were dried with sodium sulphate (2 g) and the desiccant was then filtered off. The ethyl acetate
solution was concentrated to 20 ml. To the solution trifluoroacetic acid (0.5 ml) was added and the reaction mixture was heated for 25 minutes at 50°C. After completion of the reaction, the ethyl acetate solution was extracted with 5% (w/w) aqueous solution of ammonium hydrogen carbonate. The organic phase was dried with sodium sulphate (2 g), the desiccant was filtered off and the ethyl acetate solution was concentrated to 4 ml. Crystallisation was carried out for 1 hour at 8°C. The product was then filtered off and dried at 40°C for 1 hour. The yield: 0.9' g of simvastatin in the acid form.
Example 20
Preparation of free acid of simvastatin
Simvastatin (20 g) was dissolved in the mixture of water (80 ml) and 8M KOH (18 ml). The solution was stirred in nitrogen atmosphere for two hours at room temperature. After that 120 ml of ethyl acetate was added and pH was adjusted to 2-3 with 5% aqueous solution of HCl. Ethyl acetate phase was washed with mixture of water (100 ml), 5% aqueous solution of HCl and 5% aqueous solution of NaCl (50 ml). The obtained ethyl acetate phase was then dried for three hours with addition of 20 g MgSO4 anhydrite and filtrated.
Preparation of amine salt
After that 18 ml of N-methylcyclohexylamine was added into the ethyl acetate phase. After two days at temperature between 0 and 5°C crystals of simvastatin N-methylcyclohexylamine salt were formed. Obtained crystals were filtered off and washed with 20 ml of ethyl acetate, 40 ml ethyl acetate/n-heptane (1:1) and 40 ml of pentane. The yield: 20.5 g of N-methylcyclohexylamine salt of
simvastatin. The yield calculated to the starting simvastatin was 79%.
The same results were obtained also with cyclohexylamine (by the use of the same method).
Example 21
Preparation of pravastatin in purified form
Pravastatin (30 g, HPLC purity 90.2%) in the form of Na salt was dissolved in water (100'ml) and pH was adjusted to 3 with HC1 (10% aqueous solution). The resulting free acid was extracted from the water phase into ethyl acetate (3 x 200 ml). The pooled ethyl acetate extracts were dried with sodium sulphate, the solution was filtered off and 7.604 g of N-methylcyclohexylamine was added (drop by drop at intensive stirring). After crystals were crystallised out, they were filtered off and re-crystallised from the mixture of ethyl acetate/methanol. The yield: 21 g of crystals of pravastatin N-methylcyclohexylamine salt with a HPLC purity of 99.28%.
Example 22
Preparation of free acid of atorvastatin
Calcium salt of atorvastatin (3 g) was suspended in 100 ml water. The pH was adjusted with phosphoric acid to 4 and after that free acid of atorvastatin was extracted with ethyl acetate (3 x 100 ml). Combined ethyl acetate extract was dried and 2.7 g of oil containing atorvastatin in the free acid form was obtained. Preparation of amine salt
1 g of atorvastatin in the free acid form was dissolved in acetonitrile (100 ml) and 2.1 molar equivalents of TBA was
added. The obtained mixture was concentrated to 30 ml and after four hours at 8°C the tBA salt of atorvastatin crystallised. White precipitate was filtered off and dried in a rotary evaporator. Yield: 1 g of TBA salt of atorvastatin.
Example 23
1 g of atorvastatin in the free acid form was dissolved in methanol (20 ml) and 1.1 molar equivalents of dicyclohexylamine (DCHA) in 50 ml of n-hexane was added. After four hours at 8 °C the DCHA salt of atorvastatin crystallised. White precipitate was filtered off and dried in a rotary evaporator. Yield: 1.1 g of DCHA salt of atorvastatin.







We Claim:
1. A process for preparation of the salt of a HMG-CoA reductase inhibitor with an amine, characterized in that the amine is added to a crude medium of the HMG-CoA reductase inhibitor and that said amine is selected from the group consisting of amines of the following formulae I and II.
(Formula Removed)
wherein:
al)R1, R2, R3 and R4 independently denote :
• a hydrogen atom;
• a straight or a branched alkyl group having 1 to 8 carbon
atoms;
• a cycloalkyl group having 3 to 8 carbon atoms;
• a arylalkyl group wherein the alkyl group is methyl or
ethyl and the aryl group is phenyl, which is optionally
substituted by an N-alky 1 or N, N-dialkyl group wherein
the alkyl group is alkyl having 1 to 4 carbon atoms;
• an arylalkyl group which is optionally substituted by one
or more substituents;
• a hydroxyalkyl group having 2 to 4 carbon atoms; or
• an aminoalkyl group having 2 to 4 carbon atoms, which
are optionally substituted by an N-alkyl or N, N-dialkyl
group wherein the alkyl group is alkyl having 1 to 4 carbon atoms;
• X denotes a hydrogen atom, a hydroxyl group, a halogen
or a methyl group;
• m and n independently denote an integer from 0 to 5; or
a2) NR1, R2 or NR3 R4 denote a heterocyclic ring having 3 to 7 methylene groups attached to a hydrogen atom, one of these groups being optionally substituted by an oxygen or a sulphur atom or an imine group; and X, m and n are the same as defined above;
(Formula Removed)
wherein:
bl) R'1, R'2, and R'3 are the same or different and denote hydrogen, alkyl, alkenyl, amino- or hydroxy- or alkoxy-substituted alkyl or alkenyl, or substituted amino- substituted alkyl or alkenyl, provided that R1, R'2 and R'3 are not hydrogen at the same time; or
b2) R'1 and R'2, and optionally R'3, together with the nitrogen atom form an optionally substituted heterocyclic ring system including the nitrogen atom as a ring member, and optionally including an additional hetero atom, and if R'3 is not part of the ring system it is independently selected from hydrogen, alkyl,
alkenyl, amino- or hydroxy- or alkoxy- substituted alkyl, or substituted amino- substituted alkyl; or
b3) R'1 is an optionally substituted cyclic group of general formula III,
(Formula Removed)
wherein m is zero or an integer from 1 to 5, R' is optionally substituted aliphatic hydrocarbon cyclic system having 3 to 8 carbon atoms in the ring, R'4 is hydrogen, alkyl, amino- or hydroxy- or alkoxy- substituted alkyl, substituted amino-substituted alkyl, or a group of the same general formula as R'1 as defined herein above; R1 and R'3 are the same as R'1 or hydrogen, alkyl, alkenyl, amino- or hydroxy- or alkoxy-substituted alkyl, or substituted amino- substituted alkyl or alkenyl; or
b4) R'1 is an optionally substituted aryl group of general formula IV:
(Formula Removed)
wherein R'5 is hydrogen or one or more substituents, and m is zero or an integer from 1 to 5; and R'2 and R'3 may be independently hydrogen, alkyl, amino- or hydroxy- or alkoxy-
substituted alkyl, or substituted amino- substituted alkyl, or groups of the same general formula RV
2. The process as claimed in claim 1, wherein the amine is selected from the group consisting of (±) -1, 2-dimethyl - propylamine, 3-(2-aminoethylamino)-propylamine, n-butylamine, secondary butylamine, tertiary butylamine, dibutylamine, tertiary araylamine, cyclopentylamine, cyclohexylamine, cycloheptylamine, dicyclohexylamine, N-methylcyclohexylamine, N, N' diisopropylethylenediamine, N, N' -diethylenediamine, N-methyl-1, 3-propanediamine, N-methylethylenediamine, N, N, N', N' -tetramethyl -1,2-diamino ethane, N, N, N', N' -tetramethyl -1,4-diaminobutane, N, N, N', N' - tetremethyl - 1, 6 diaminohexane, 1, 2-dipiperidinethane, dipiperidinemethane, 2-amino-3, 3-dimethylbutane, N, N-dimethylcyclohexylamine, neopentylamine, adamantylamine, N, N-diethylcyclohexylamine, N-isopropylcyclo-hexylamine, N-methylcyclohexylamine, cyclobutylamine and norborylamine.
3. The process as claimed in claim 1, wherein the amine is selected from the group consisting of n-butylamine, secondary butylamine, tertiary butylamine, dibutylamine, tertiary amylamine, cyclohexylamine, dicyclohexylamine, N-methylcyclohexylamine and N,N' - diisopropylethylenediamine.
4. The process as claimed in any one of claims 1 to 3, wherein the HMG-CoA reductase inhibitor is selected from the group
consisting of mevastatin, pravastatin, lova-statin, simvastatin, fluvastatin and atorvastatin.
5. The process as claimed in any one of claims 1 to 4, wherein said crude medium is present in an organic solvent which is selected from the group consisting of ethyl acetate, ether and acetonitrile.
6. The process as claimed in any one of claims 1 to 5, wherein the HMG-CoA reductase inhibitor in said crude medium is in the acid form.
7. The process as claimed in any one of claims 1 to 6, comprising the following steps:

a) Contacting the medium containing the HMG-CoA reductase inhibitor with the amine,
b) optionally: conventionally forming crystallization nuclei,
c) filtering the crystals crystallized out,
d) washing the crystals with an organic solvent, and
e) drying the crystals.
8. The process as claimed in claim 7, wherein the crystallization is
carried out at temperature between 0 and 30° C.
9. The process as claimed in claim 8, wherein the crystallization is carried out at temperature between 4 and 22° C.
10. A process for the isolation and/or purification of a HMG-CoA
reductase inhibitor, characterized in that the amine salt of the
HMG-CoA reductase inhibitor as defined in any of the preceding
claims is used for preparing and isolating the HMG-CoA reductase
inhibitor by means of crystallisation.
11. The process as claimed in claim 10, wherein the amine salt of
mevastatin, pravastatin, lovastatin, simvastatin, fluvastatin or
atorvastatin is prepared and isolated.
12. The process as claimed in claim 11, wherein the tertiary
butylamine salt of mevastatin, pravastatin, lovastatin, simvastatin,
fluvastatin or atorvastatin is prepared and isolated.


Documents:

in-pct-2001-00020-del-abstract.pdf

in-pct-2001-00020-del-claims.pdf

in-pct-2001-00020-del-correspondence-others.pdf

in-pct-2001-00020-del-correspondence-po.pdf

in-pct-2001-00020-del-description (complete).pdf

in-pct-2001-00020-del-form-1.pdf

in-pct-2001-00020-del-form-13.pdf

in-pct-2001-00020-del-form-19.pdf

in-pct-2001-00020-del-form-2.pdf

in-pct-2001-00020-del-form-3.pdf

in-pct-2001-00020-del-form-5.pdf

in-pct-2001-00020-del-gpa.pdf

in-pct-2001-00020-del-petition-138.pdf


Patent Number 244825
Indian Patent Application Number IN/PCT/2001/00020/DEL
PG Journal Number 52/2010
Publication Date 24-Dec-2010
Grant Date 21-Dec-2010
Date of Filing 11-Jan-2001
Name of Patentee LEK PHARMACEUTICAL D.D.
Applicant Address VEROVSKOVA 57,1526 LJUBLJANA,SLOVENIA
Inventors:
# Inventor's Name Inventor's Address
1 ZLATKO PFLAUM CES MINOVA 25,1230 DOMZALE, SLOVENIA,
PCT International Classification Number C07C 211/07
PCT International Application Number PCT/IB99/01554
PCT International Filing date 1999-09-17
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 P-9800240 1998-09-18 Slovenia