Title of Invention

INJECTABLE CALCIUM PHOSPHATE SOLID RODS AND PASTES FOR DELIVERY OF OSTEOGENIC PROTEINS

Abstract Osteogenic proteins are delivered via an injectable solid rod or hardenable paste. The formulation comprises a calcium phosphate material, an osteogenic protein, and optional additives and active ingredients such as a bone resorption inhibitor. Methods of making injectable pharmaceutical compositions and methods of using the osteogenic compositions to treat bone defects are also disclosed.
Full Text WO 2005/025595 PCT/US2004/029560
polyoxyethylene oxide, carboxyvinyl polymer, polyvinyl alcohol, dextran sulfate and combinations thereof. The additive is preferably present in an amount ranging from about 1% to about 90%, more preferably from about 20% to-about 40%, by weight o the rod-shaped osteogenic composition and in an amount ranging from about 1% to about 90%, more preferably about 10% to about 20%, by weight of the hardenable paste osteogenic composition.
[0012] When the osteogenic composition of the present invention takes the form of a solid cylindrical rod, the diameter of the cylindrical rod is preferably between about 0.1 mm and 3.0 mm, more preferably about 1.0 mm, and the length of the cylindrical rod is preferably between about 0.5 cm and 5.0 cm.
[0013] An additional embodiment of the present invention is directed to a method for preparing a rod-shaped composition for injectable delivery of osteogenic proteins, said composition comprising an osteogenic protein and a calcium phosphate material, comprising the steps of (a) mixing a dry form of the osteogenic protein with a-dry form of the calcium phosphate material to produce a dry mixture; (b) reconstituting the dry mixture by adding an aqueous buffer to form a paste; (c) molding the paste to form a rod shaped composition; and (d) drying the rod shaped composition of step (c) to form a rod-shaped composition for injectable delivery of osteogenic proteins. In preferred embodiments , the aqueous buffer is selected from phosphate-buffered saline, saline, glycine- and glutamic acid-based buffers and combinations thereof. The volume to weight (mL:g) ratio of aqueous buffer to dry mixture ranges from about 0.5:1 to about 2:1. Molding is preferably accomplished by molding, extruding, pressing, boring or combinations thereof. In certain preferred embodiments , the rod-shaped composition is cut before or after step (d).
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[0014] Yet another embodiment of the present invention is directed to a method for preparing a paste composition for injectable delivery-of osteogenic proteins, said composition comprising an osteogenic protein and a calcium phosphate material, comprising the step of mixing a dry form of the calcium phosphate material with an aqueous buffer containing an -osteogenic protein to form a paste. In preferred embodiments of the present invention, the aqueous buffer is selected from phosphate-buffered saline, saline, glycine- and glutamic acid-based buffers and combinations thereof. The ratio of aqueous buffer to calcium phosphate material ranges from about 0.5:1 to about 2:1.
[0015] Still further embodiments of the present invention are directed -to methods of treating a mammal having a-bone-defect comprising administering to the site of the bone defect an effective amount of a composition for injectable delivery of osteogenic proteins claimed herein. Still further embodiments of the present invention are directed to methods of treating a mammal having a bone-defect comprising the steps of (a) administering to the site of the bone defect an effective amount of a composition for injectable delivery of osteogenic-proteins-claimed herein and (b) administering to the site of the bone defect an effective amount of a bone resorption inhibitor. The administration of a bone nesorption inhibitor can be performed prior to step (a), after step (a) or simultaneously with step (a)
BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 is a graph showing the in vitro release kinetics of rhBMP-2 from a-BSM rods using 125l-rhBMP-2 as a tracer.
[0017] Figure 2 is a graph showing the in vivo local retention of rhBMP-2 from a-BSM rods using 125l-rhBMP-2 as a tracer.
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DETAILED DESCRIPTION OF THE INVENTION
[0018] Generally, the methods and compositions of the present invention relate to the regeneration of bone tissue and concomitant increase of bone mass, bone density, and bone strength. More particularly, the subject invention involves injectable solid rods and pastes comprising an osteogenic protein, a calcium phosphate carrier and optional additives and active agents such as a bone resorption inhibitor, as well as methods of preparing such osteogenic compositions and methods of treating using such osteogenic compositions. The-calcium phosphate solid rods and hardenable pastes of the present invention are suitable for intraosseous delivery of osteogenic proteins. Through the use of the present inventive methods and compositions, the severity of osteoporosis or the incidence of osteoporotic lesions can be advantageously lessened, ultimately lessening the incidence of bone fractures. Other clinical uses include fracture repair, cartilage repair, non-union defect repair and spinal fusion. The injectable solid rods and hardenable pastes of the present invention may also be used to promote bone growth as needed when using joint replacement implants as described in provisional U.S. Application No. 60/502,526, ("Promotion of Bone Ingrowth in Joint Replacement Implants Using Osteogenic Proteins; the subject matter of this provisional application is incorporated in its entirety by reference herein.
[0019] A first embodiment of the present invention is directed to a rod-shaped composition for injectable delivery of osteogenic proteins comprising an osteogenic protein and a calcium phosphate material. A composition according to this first embodiment of the present invention may optionally include other additives (binders, excipients) and/or active agents such as a bone resorption inhibitor.
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[0020] This solid rod-shaped composition is suitable for local intraosseous delivery and may, therefore, be injected directly into an osteoporotic or osteopenic site to effectively induce the formation and/or maintenance of bone. Further, the injectable rod-shaped composition exhibits a sustained-release profile with regard to the osteogenic protein when delivered in this manner. Preferably the solid rods of the present invention are cylindrical and have a diameter ranging from about 0.1 mm to about 3.0 mm, more preferably about 1.0 mm so as to allow delivery by a 16-gauge needle. In addition, the solid rods preferably have a length ranging from about 1.0 mm to about 5.0 cm.
[0021] Unlike existing injectable formulations, the osteogenic composition of this first embodiment of the present invention is administered in a solid form, thereby avoiding the deficiencies inherent in liquid or viscous formulations. For example, using liquid or gel formulations, the osteogenic agent may be prematurely diluted by the body fluids before the bone promoting effect can be achieved. The present invention obviates the dilution effect-by employing a solid-carrier which degrades slowly in vivo, thereby providing delayed, sustained release of the active agent(s). Furthermore, unlike liquid or viscous formulations which may migrate from the-site of administration, the solid compositions of the present invention become lodged and persist at the site of desired bone growth to effect the bone growth promoting activity. Also they allow a more precise injectable placement .of a solid rod in areas of low bone mass. Typically, the composition should persist at the site for a period from about 5 days to about 2 months. If the composition is dispersed prematurely, the desired bone growth promotion effect either will not occur or the formed bone will not have the desired strength. Finally, although the osteogenic composition of this embodiment of the present invention is administered as a solid, it is preferably
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formed as a cylindrical rod, thereby being suitable for either injection or implantation into the body. Of course, if desired, other rod shapes could be used, e.g. hexagonal, square or semi-circular rod shapes. In addition, the well known surgical complication of inducing an embolism during an intraosseous injection procedure is considerably mitigated through the use of solid rods (vs. liquid or gel forms). The potential displacement of intraosseous bone fragments, fat or an embolism caused by a pressurized injection of a large volume of liquid/gel carrier is reduced since the volume injected of highly concentrated solid rod is much less than that required if a similar dose was dispensed in a liquid or gei form. The composition may be applied to the site of desired bone growth in any convenient manner, including by introduction through a conventional hypodermic needle or syringe.
[0022] A second embodiment of the present invention is directed to a method for preparing the solid rod-shaped composition for injectable delivery of osteogenic proteins. In the first step, a dry form of the osteogenic protein is mixed with a dry form of the calcium phosphate material to produce a dry mixture. In other words, powdered or dry forms of both the osteogenic protein and the calcium phosphate material are initially employed in order to form a dry mixture. When additives and/or additional active agents are included in the composition, these materials may also be employed in a dry or powdered form and included in the dry mixture.
[0023] In the second step, the dry mixture is reconstituted by adding an aqueous buffer to form a paste. Suitable aqueous buffers include, without limitation phosphate-buffered saline, saline, glycine-based buffers and combinations thereof. When using BMP-2 as the osteogenic protein, a glycine-based buffer having a pH of about 4.5 is preferred for use; more preferably, a glycine-based duffer having a
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composition of 5 mmol L-giutamic acid, 2.5% glycine, 0.5% sucrose, 5 mmol NaCI and 0.01% polysorbate 80 is used.
[0024] A volume to weight (mL:g) ratio of aqueous buffer to dry mixture ranges from about 0.5:1 to about 2:1. However, the lower limit of this weight ratio is limited only by the concern that enough liquid be added to the dry mixture so as to be sufficient to allow the formation of a paste which can be formed through injection through a syringe or some other method. In addition, the upper limit of this weight ratio is limited only by the concern that not so much liquid be added to the dry mixture that, upon subsequent drying, the geometry achieved, i.e., rod shape, is disturbed; in other words, if too much liquid is used, then the rod shape formed in the third step of this method will be compromised upon drying the osteogenic compound.
[0025] This step is performed under conditions where-substantially uniform mixing occurs. Mixing combines the ingredients and can be used-to regulate the extent of inter-ingredient reactions. While all of the desired ingredients are preferably contained in the dry mixture, it is also possible to add an additive or an additional active agent immediately prior to the initiation of mixing or prior to the completion of mixing. Such an additive or additional active agent is preferably in a dry form; however, a hydrated form of the additive or additional active agent may also be added to the paste.
[0026] In the third step of the present inventive method, the paste is molded to form a rod shaped composition. Molding or shaping may be accomplished using any one of a number of known techniques such as molding, extruding, pressing, boring and/or cutting. In a preferred embodiment of this invention, the paste is packed into and extruded through the hub end of a hypodermic syringe. !nthis case, the plunger of the syringe is inserted and a sufficient amount of pressure is applied
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to extrude a continuous length of paste onto a dry surface. Sections are then-cut using a cutting tool such as a razor, scalpel, knife or the like, to form injectabte, rod-shaped compositions. Cutting may also take place after the drying step described below. Alternatively, the paste may be packed into a cylindrical mold, catheter, air or gas-permeable tubing (e.g., silastic or Teflon®/FEP), or any other extrusion-type apparatus.
[0027] In the final step, the rod shaped composition obtained in the previous step is dried or hardened to form the rod-shaped composition for injectable delivery of osteogenic proteins of the present invention (first embodiment). Drying may be accomplished via air drying or incubation at elevated temperatures, i.e., at least 37°C. The drying temperature is limited only by a concern for osteogenic protein degradation, which typically occurs somewhere in the range of 55°C and 60cC. When drying is accomplished in a 37°C oven, drying takes approximately at least one hour, and preferably drying is carried out overnight. The rod-shaped composition preferably has a residual moisture of less than 10%.
[0028] A third embodiment of the present invention is directed to a
hardenable paste composition for injectable delivery of osteogenic proteins
comprising an osteogenic protein and a calcium phosphate material. A composition
according to this third embodiment of the present invention may optionally include
other additives (binders, excipients) and/or active agents such as a bone resoiption
inhibitor.
[0029] This paste is suitable for local intraosseous delivery and may, therefore, be injected directly into an osteoporotic or osteopenic site whereupon the paste hardens to a solid form and effectively induces the formation and/or maintenance of bone. Because the paste hardens upon injection into an at teast
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37°C environment, i.e., into a mammal, many of the disadvantages associated with the use of liquid or gel compositions are avoided. Similar to the solid rod-shaped injectable composition described above, the hardened paste -composition exhibits a sustained-release profile with regard to the osteogenic protein. The injectable life of the hardenable paste may be prolonged by refrigerating the paste.
[0030] A fourth embodiment of the present invention is directed to a method for preparing the paste composition for injectable delivery of osteogenic proteins. In this method, a dry form of the calcium phosphate material is mixed with an aqueous buffer containing an osteogenic protein to form a paste. When additives and/or additional active agents are included in the composition, these materials may be employed in either dry form to be pre-mixed with the dry form of calcium phosphate material or in a hydrated form to be mixed directly with the aqueous buffer and the calcium phosphate material. Mixing is performed under conditions where substantially uniform mixing occurs. Mixing combines the ingredients and can be used to regulate the extent of inter-ingredient reactions.
[0031] Suitable aqueous buffers include, without limitation, phosphate-buffered saline, saline, glycine-based buffers and combinations thereof. When using BMP-2 as the osteogenic protein, a glycine-based buffer having a pH of about 4.5 is preferred for use; more preferably, a glycine-based buffer having a composition of 5 mmol L-glutamic acid, 2.5% glycine, 0.5% sucrose, 5 mmol NaCI and 0.01% pplysorbate 80 is used. A weight ratio of aqueous buffer to dry calcium phosphate ranges from about 0.5:1 to about 2:1.
[0032] Alternatively, a dry form of the osteogenic-protein is mixed with a dry form of the calcium phosphate material to produce a dry mixture, and the dry mixture is then reconstituted by adding an aqueous buffer to form a-paste. These steps
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would be identical to the first and second steps of the method of preparing the solid rod-shaped composition above-(second embodiment) except that a weight ratio of aqueous buffer to dry calcium phosphate ranges from about 0.5:1 to about 2:1.
[0033] Details regarding the active agent, carrier, additives and bone resorption inhibitors suitable for use in the present invention are given below.
ACTIVE AGENT
[0034] The active agent present in the osteogenic compositions of the present invention is preferably selected from the family of proteins known as the transforming growth factors beta (TGF-b) superfamily of proteins. This family includes the activins, inhibins and bone morphogenetic proteins (BMPs). These .BMPs include BMP-proteins BMP-2, BMP-3, BMP-4, BMP-5, BMP-6, and BMP-7, disclosed, for example, in U.S. Patent No. 5,108,922, 5,013,649, 5,116,738, 5,106,748, 5,187,076, and 5,141,905, BMP-8, disclosed in PCT WO 91/18098, BMP-9, disclosed in PCT WO 93/00432, BMP-10, disclosed in PCT WO 94/26893, BMP-11, disclosed in PCT WO 94/26892, BMP-12 and BMP-13, disclosed in PCT WO 95/16035, BMP-15, disclosed in U.S. Patent No. 5,635,372, and BMP-t6, disclosed in U.S. Patent No. 6,331,612. Other TGF-b proteins which may be useful as the active agent in the present invention include Vgr 2, Jones et al., Mol. Endocrinol., vol. 6, pp. 1961 1968 (1992), and any of the growth and differentiation factors (GDFs) including those described in PCT WO 94/15965, WO 94/15949, WO 95/01801, WO 95/01802, WO 94/21681, WO 94/15966, WO 95/10539, WO 96/01845, WO 96/02559 and others. Also useful in the present invention may be BIP, disclosed in WO 94/01557, HP00269, disclosed in JP 7 250688, and MP52, disclosed in PCT WO 93/16099. The disclosures of all of the above patents, journal
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publications and published international applications are incorporated-by reference herein.
[0035] Preferably, the active agent includes at least one protein selected from the subclass of proteins known generally as BMPs, which have been disclosed to have osteogenic activity, and other growth and differentiation type activities. A subset of BMPs which are presently preferred for use in the present invention include BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-10, BMP-12, and BMP-13, most preferably BMP-2, the sequence of which is disclosed in U.S. Patent No. 5,013,649, the disclosure of which is incorporated by reference herein.
[0036] The active agent may be recombinantly produced or purified from a protein composition. The active agent, if a TGF-b such as a BMP, or other dimeric protein, may be homodimeric or may be heterodimeric with other BMPs (e.g., a heterodimer composed of one monomer -each of BMP-2 and BMP-6) or with other members of the TGF-{5 superfamily, such as activins, inhibins and TGF-b1 (e.g., a heterodimer composed of one monomer each of a BMP-and a related member of the TGF-b superfamily). Examples of such heterodimeric proteins are described, for example, in PCT WO 93/09229, the disclosure of which is incorporated by reference herein. The active agent may comprise DNA encoding for BMPs and cells transduced or transfected with genes encoding BMP-proteins.
[0037] The active agent may further comprise additional agents such as the Hedgehog, Frazzled, Chordin, Noggin, Cerberus and Follistatin proteins. These families of proteins are generally described in Sasai et al., Cell, vol. 79, pp. 779-790 (1994) (Chordin); PCT WO 94/05800 (Noggin); and Fukui et al., Dev. Biol., vol. 159, pp. 131-139 (1993) (Follistatin). Hedgehog proteins are-described in PCT WO 96/16668, WO 96/17924, and WO 95/18856. The Frazzled family of proteins is a
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relatively recently discovered family of proteins with high homology to the extracellular binding domain of the receptor protein family known as Frizzled. The Frizzled family of genes and proteins is described in Wang et al., J. Biol. Chem., vol. 271, pp. 4468-4476 (1996). The active agent may also include other soluble receptors, such as the truncated soluble receptors disclosed in PCT WO 95/07982. From the teaching of WO 95/07982, one skilled in the art will recognize that truncated soluble receptors can be prepared for numerous other receptor proteins. Such would also be encompassed within the present invention. The above publications are hereby incorporated by reference herein.
[0038] The amount of active agent useful herein is that amount effective to stimulate increased osteogenic activity of present or infiltrating progenitor {osteoblast precursor cellsu) or other cells (hereinafter "effective amount") and will depend upon the size and nature of the defect being treated, as well as the composition of the calcium phosphate carrier being employed. Generally, the amount of osteogenic protein present in a solid rod-shaped osteogenic-composition of the present invention ranges from about 1 % to about 90%, more preferably from about 15% to about 40%, by weight of the osteogenic composition. Generally, the amount of osteogenic protein present in a hardenable paste osteogenic composition of the present invention ranges from about 0.01% to about 2%, more preferably from about 0.03% to about 1%, by weight of the osteogenic-composition.
CARRIER
[0039] According to all embodiments of the present invention, a calcium phosphate material is employed as a carrier. As used herein, a "calcium phosphate material" means any synthetic bone substitute material comprising calcium phosphate as the primary component, i.e., having at least 90% by weight attributable
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to calcium and/or phosphate. The calcium phosphate material of the present invention may be any biocompatible, calcium phosphate material known in the art. Suitable calcium phosphate materials may be produced by any one of a variety of methods and using any suitable starting components or may be commercially available.
[0040] In certain preferred embodiments of the present invention, the calcium phosphate material is present in an amount ranging from about 10% to about 99%, more preferably from about 40% to about 60%, by weight of the solid rod-shaped osteogenic composition of the present invention, in certain other preferred embodiments of the present invention, the calcium phosphate material is present in an amount ranging from about 30% to about 70%, more preferably from about 45% to about 55%, by weight of the hardenable paste osteogenic composition of the present invention. Preferably, the calcium phosphate material or carrier is used in dry, i.e., powdered, form.
[0041] Forms of calcium phosphate suitable for use in this invention include, without limitation, amorphous apatitic calcium phosphate (ACP), poorly crystalline apatitic calcium phosphate (PCA), hydroxyapatite (HA), tricalcium phosphate and fluorapatite. In a preferred embodiment, the calcium phosphate material is a poorly crystalline apatitic calcium phosphate solid having a calcium-to-phosphate (Ca/P) ratio comparable to naturally occurring bone minerals, more preferably a calcium-to-phosphate ratio of less than about 1:1.5, most preferably about 1:1.4.
[0042] Suitable PCA materials may be identified by-combining PCA precursors, hydrating with a limited amount of water (so that a paste or putty is formed), molding into a cylindrical rod shape, and allowing the molded materiaUo harden into a PCA material. Desirable precursors are capable of hardening in a
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moist environment, at or around body temperature in less than 5 hours and preferably within 10-30 minutes.
[0043] According to the present invention, the calcium phosphate carrier may comprise any bone substitute material which contains one of the above forms of calcium phosphate as its primary component, i.e. having at least 90% by weight attributable to calcium and/or phosphater The bone substitute material may comprise solely one of the above forms of calcium phosphate, with or without additional components; the bone substitute may comprise a combination -of the above forms of calcium phosphate/with or without additional components. In addition, one or more of the above-noted forms of calcium phosphate may be used to prepare a calcium phosphate material suitable for use in the present invention. Methods of making such materials are well known in the art. However, any method which results in obtaining a dry calcium phosphate material, i.e., powdered, is appropriate.
[0044] As used herein, "amorphous" means a material with significant amorphous character. Significant amorphous character contemplates greater than 75% amorphous content, preferably greater than 90% amorphous content, and is characterized by a broad, featureless X-ray diffraction pattern.
[0045] "Poorly crystalline apatitic calcium phosphate," PCA calcium phosphate" and "PCA material," as those terms are used herein, describe a-synthetic poorly crystalline apatitic calcium phosphate. The poorly crystalline apatitic (PCA) material is not necessarily restricted to a single calcium phosphate phase provided it has the characteristic X-ray diffraction (XRD) and fourier transform infrared (FTIR) pattern. A PCA calcium phosphate has substantially the same XRD spectrum as bone. The XRD spectrum is generally characterized by only two-broad peaks in the
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region of 20-35E with one centered at 26E and the other centered at 32E. The FTIR spectrum is characterized by peaks at 563 cm-1-,1034 cm-1,1638 cm-1 and 3432 cm-1 (±2 cm-1); sharp shoulders are observed at 603 cm-1 and 875 cm-1, with a doublet having maxima at 1422 cm-1 and 1457 cm-1. PCA materials preferred for use in the present invention are described in U.S. Patent Nos. 5,650,176, 5;683,461 and 6*214,368, each of which is incorporated by reference herein. Suitable materials are also described in a set of related applications, entitled "Delivery Vehicle," "Conversion of Amorphous Calcium Phosphate to Form a Novel Bioeeramic," "Orthopedic and Dental Ceramic Implants," and "Bioactive Ceramic Composites," each of which was filed on October 16,1997 and assigned to ETEX Corporation (Cambridge, MA) and is incorporated by reference herein. In light of the breadth of disclosure in each of these above-noted patent documents, details of the production of suitable PCA materials will not be detailed here. A summary of PCA characteristics will suffice. PCA material is characterized by its bior-esor-bability and its minimal crystallinity. Its crystalline character is substantially the same as natural bone. PCA material also is biocompatible and not detrimental to the host.
[0046] Crystalline hydroxyapatite (HA) is described, for example, in U.S. Patent Re. Nos. 33,221 and 33,161, both of which are incorporated-by reference herein. These patents teach the preparation of calcium phosphate remineralizatioh compositions and of a finely crystalline, non-ceramic, gradually resorbabje hydroxyapatite carrier material based on the same calcium phosphate composition. A similar calcium phosphate system, which consists of tetracalcium phosphate (TTCP) and monocaieium phosphate (MCP) or its monohydrate form (MCPM), is described in U.S. Patent Nos. 5,053,212 and 5,129,905, both of which are incorporated by reference herein. Additional crystalline HA materials (commonly
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referred to as dahllite) are described in U.S. Patent No. 5,'962,028, the disclosure of which is incorporated by reference herein.
ADDITIVES
[0047] Additives may be useful in the osteogenic compositions of the present invention. Many such binders, which enhance cohesiveness, and excipients, which stabilize and/or modulate release of active ingredients, are well known in the formulation art. Suitable additives include, without limitation, pharmaceutically acceptable salts, polysaccharides, peptides, proteins, amino acids, synthetic polymers, natural polymers, and/or surfactants. Useful polymers include, for example, those described in U.S. Patent No. 5,171,579, the entire disclosure of which is incorporated by reference herein. Preferred additives include oellulosic materials such as carboxymethylcellulose (CMC), hydroxypropylmethylcellulose (HPMC), and methylcellulose (MC), synthetic polymers such as polylactides and polyethylene glycols, e.g., polylactide/polyethylene glycol, polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), polyoxyethylene oxide, carboxyvinyl polymer and polyvinyl alcohol (PVA), and-dextran sulfate and combinations thereof. Other useful additives include, without limitation, sodium alginate, chitosan, collagen, gelatin, hyaluronan, and various peptides, proteins, and amino acids. Additives which have an effervescent effect are not presently intended for use in the present invention. [0048] In a preferred embodiment of the present invention, an additive is employed in a dry or powder form, which is mixed with the active agent(s), carrier and aqueous liquid in order to prepare the injectable composition of the present invention. In certain preferred embodiments of the present invention, additive(s) is present in an amount ranging from about 1% to about 90%, more preferably from about 20% to about 40%, by weight of the-solid rod osteogenic-composition. In
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certain preferred embodiments of the present invention, additive(s) is present in an amount ranging from about 1 % to about 90%, more preferably from about 10% to about 20%, by weight of the hardenable paste osieogenic composition.
ADDITIONAL ACTIVE AGENTS
[0049] The injectable osteogenic compositions of the present invention may also include an additional active agent or additional active agents. Such additional active agents may be mixed, preferably in dry form though hydrated forms are also suitable for use, with the active agent, carrier and aqueous liquid in order to prepare the injectable osteogenic compositions of the present invention. Alternatively, such additional active agents may be co-administered with the osteogenic compositions of the present invention, either in some sequential manner or simultaneously (hereinafter "co-administration scheme"). Additional active agents may be employed herein in order to achieve additional desired effects or, in some cases, may be employed to counter potential undesirable effects, such as infection, inflammation and transient resorption.
[0050] For example, although much is known about the osteogenic potential of TGF-(3 proteins, recent reports show that local administration of certain osteoinductive agents, such as BMP-2, stimulates transient osteoclastic activity (local areas of bone resorption) at the site of administration. This reaction, which occasionally precedes new bone formation induced by the BMP, has been termed "transient resorption phenomenon." Agents known to inhibit bone resorption may, therefore, play an important role in delaying or reducing the initial bone resorption associated with local BMP-administration, without inhibiting subsequent bone formation.
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[0051] Thus, in preferred embodiments of the present invention, a bone resorption inhibitor is employed as an additional active agent present in the injectable osteogenic composition or is co administered with the injectable osteogenic composition in order to prevent or minimize the initial bone resorption associated with intraosseous delivery of an active agent such as BMP. As used herein, the term "inhibition of bone resorption" refers to prevention of bone loss, especially the inhibition of removal of existing bone through direct or indirect alteration of osteoclast formation or activity. Thus, the term "bone resorption inhibitor" as used herein refers to agents that prevent or inhibit bone loss by the direct or indirect alteration of osteoclast formation or activity.
[0052] In certain preferred embodiments, the bone resorption inhibitor is a biphosphonate. As used herein, the term "bisphosphonate" refers to the related bisphosphonic acids and salts, and various crystalline and amorphous forms of bisphosphonate. Clinically, bisphosphonate therapy has been shown to dramatically reduce indices of bone turnover, increase bone mineral density, and, in osteopenic women, reduce hip and spine fracture risk (see, for example, H. Fleisch, Bisphosphonates in Bone Disease, from the Laboratory to the Patient, 3rd ed., Parthenon Publishing (1997), which is incorporated by reference herein).
[0053] Biphosphohates suitable for use in the present invention include, without limitation, alendronate, cimadronate, clodronate, EB 1053, etidronates, ibandronate, neridronate, olpadronate, pamidronate, risedronate, tiludronate, YH 529, zoledronate, and pharmaceutically acceptable salts, esters, acids, and mixtures thereof. The amount of biphosphonate, and indeed the amount of any bone resorption inhibitor, useful is that amount effective to prevent or inhibit the transient bone loss occasionally associated with the local administration of an osteogenic
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protein such as BMP-(hereinafter "effective amount"), by the direct or indirect alteration of osteoclast formation or activity. The precise dosage necessary will depend upon the size and nature of the bone defect being treated, as well as the amount of osteogenic agent being delivered. Generally, the amount of biphosphonate to be delivered is preferably in a range of from about 0.1 to about 3000 mg, more preferably about 10 to about 1000 mg, and most preferably about 10 to about 500 mg per cubic centimeter of material.
[0054] When the co-administration scheme of the present invention is employed, the bone resorption inhibitor is typically delivered in a suitable carrier. The carrier may be any pharmaceutically acceptable carrier, a wide variety of which are well known and readily available in the art (see, for example, Martin, E.W., Remington's Pharmaceutical Sciences (Mack Pub. Co., current edition), which is hereby incorporated by reference herein). Presently preferred carriers are formed into solid rods or pastes, as described elsewhere herein.
[0055] In the co-administration scheme, the bone resorption inhibitor may be administered sequentially, either before or after, or concurrently with the injectable solid rod or paste compositions of the present invention. In addition, the-bone resorption inhibitor may be applied locally (intraosseous), but may be applied to other parenteral sites such as intramuscular or subcutaneous or ingested orally or injected intravenously for systemic delivery. Preferably the bone resorption inhibitor, e.g., a bisphosphonate, is delivered systemically, i.e., orally or intravenously, ¦either before or concurrently with the injectable solid rod or paste composition of the present invention. Further, the bone resorption inhibitor may be implanted at the site to be treated by surgical implantation. It should be noted, however, that-despite their therapeutic benefit, bisphosphonates are poorly absorbed in the gastrointestinal tract

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when taken orally. To overcome this poor bioavailability issue, intravenous administration has been used; however, this modality is seen as costly and inconvenient due to the duration and frequency of dosing. The present invention, therefore, can overcome this deficiency by incorporating the -bisphosphonate within the injectable osteogenic compositions of the present invention and delivering it locally directly to the site of desired action.
[0056] The above description related to the administration of a bone resorption inhibitor relates generally to the administration of any additional active agent. Other suitable additional active agents include, without limitation, other osteogenic proteins, antibiotics, anti-inflammatory agents, growth factors, peptides, proteins, cytokines, oligonucleotldes, antisense oligonucleotides, DNA and-polymers. These compounds may be added, preferably in dry form, by mixing them to form the paste in the preparation methods of the present invention.
ADMINISTRATION
[0057] According to the present invention, methods and compositions are provided for treatment of patients who exhibit bone defects. As used herein, "bone defects" include osteoporotic bone, osteopenic bone, bone fracture, cartilage defect, and any other bone- or cartilage-related condition which would be improved or corrected with stimulated bone or-cartilage growth. Therefore, a fifth embodiment of the present invention is directed to a method of treating a mammal having a-bone defect by administering to the site of the bone defect an effective amount of an injectable rod or paste composition of the present invention.
[0058] A related sixth embodiment of the present invention is directed to a method of treating a mammal having a bone defect by administering to the-site-of the bone defect an effective amount of an injectable rod or paste composition of the
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WO 2005/025595 PCT/US2004/029560
present invention and by administering an effective amount of a bone resorption inhibitor. The bone resorption inhibitor may also be administered to the site of the bone defect, but can be administered by some other route, i.e., parenteral, surgical
implantation, oral, intravenous. In addition, the administration of the bone resorption. ¦
inhibitor may occur prior to, concurrent with or after the administration of the
injectable rod or paste composition of the present invention.
[0059] Most commonly, the methods and compositions of the present invention are provided for the treatment of patients who exhibit signs of osteoporosis or osteopenic conditions, including osteoporotic bone lesions. The identification-of such patients may be accomplished by procedures which are well known in the art. Such procedures provide the clinician with information on the location and severity-of osteoporotic or osteopenic bone lesions. In addition to locating the lesion(s) to be treated, the clinician can use this information to select the appropriate mode of administration and dose of osteoinductive agent for the patient. -Useful diagnostic procedures include measurement of bone mass/density using dual energy X ray absorptiometry (DEXA), Kilgus, et al., J. Bone & Joint Surgery, vol. 75 B, pp. 279-287 (1992); Markel, et al., Acta Orthop. Scand., vol. 61, pp. 487 498 (1990); and quantitative computed tomography (QCT), Laval Jeantet, et al., J. Comput. Assist. Tomogr., vol. 17, pp. 915-921 (1993); Markel, Calcif. Tissue Int., vol. 49, pp. 427-432 (1991); single photon absorptiometry, Markel, et al. Calcif. Tissue Int., vol. 48, pp. 392-399 (1991); ultrasound transmission velocity (UTV); Heaney, et al., JAMA, vol. 261, pp. 2986-2990 (1989); Langton, et al., Clin. Phys. Physiol. Meas., vol. 11, pp. 243-249 (1990); and radiographic assessment, Gluer, et al., J. Bone Min. Res., vol. 9, pp. 671-677 (1994). Other methods of identification of patients at risk of bone fracture include assessment of age related factors, such as cognisance, as well as
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WO 2005/025595 PCT/US2004/029560
prior occurrence of osteoporosis related fractures. Porter, et al., BMJ, vol. 301, pp. 638-641 (1990); Hui, et al., J. Clin. Invest., vol. 61, pp. 1804-1809 (1988). The above publications are hereby incorporated by reference herein.
[0060] Particular dosage regimens will be determined by the clinical indication being addressed, as well as by various patient variables (e.g weight, age, sex) and clinical presentation (e.g. extent of injury, site of injury, etc.).
[0061] The injectable osteogenic compositions of the present invention may be administered in any clinically acceptable manner of injection. A number of commercially available syringes may be suitable for use in the present invention, and for administration of the compositions of the present invention. Such syringes include, without limitation, the Calasept® syringe (JS Dental Manufacturing, Ridgefield CT); the Henke-Ject® aspirating syringe and Hypo® dental syringes/needles (Smith & Nephew MPL, FranWin Park, IL); intraosseous needles from MPL, Inc., Chicago IL; and Luer-Lok® Syringes (Becton Dickinson, Franklin Lakes, NJ). Any syringe capable of holding and delivering a paste or an injectable rod and/or enabling extrusion with an obdurator is appropriate for use.
[0062] In one embodiment of the invention, the solid rod shaped compositions are delivered intraosseously using an appropriate size and type hypodermic needle percutaneously or surgically preplaced into the -selected anatomic location. Percutaneous placement of the hypodermic needle may -be accomplished using manual palpation of known anatomic landmarks, with or without the use of fluoroscopy for visualize placement. Fluoroscopy may also-be used in conjunction with surgical implantation prior to and/or concurrent with placement of the hypodermic needle.
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WO 2005/025595 PCT/US2004/029560
[0063] In a preferred embodiment, a guide wire (commonly referred to as a "k wire") is first inserted percutaneously into the desired anatomic location to serve as a guide for the hypodermic needle. The hypodermic needle is inserted over the guide wire, which is subsequently removed leaving only the hypodermic needle in place. The solid rod shaped composition is then inserted into the hub end of the hypodermic needle. Following loading of the composition, a second guide wire is inserted into the needle, which is used to advance the solid composition to ttie tip of the needle. The needle is then removed leaving the guide wire to anchor the composition within the bone at the desired location. Finally, the guide wire is removed leaving the solid composition in place. In another embodiment, the solid rod shaped composition of the invention is preplaced within the needle barrel. After placement into the desired anatomic site, the plunger of the syringe is advanced into the needle barrel as the device is withdrawn, leaving the solid rod shaped composition at the desired location.
[0064] The compositions of the subject invention allow therapeutically effective amounts of osteogenic protein to be delivered to an injury site where cartilage and/or bone formation is desired. The formulations may be used as a substitute for autologous bone graft in fresh and non-union fractures, spinal fusions, and bone defect repair in the orthopaedic field, in cranio/maxillofacial reconstructions, in osteomyelitis for bone regeneration, and in the dental field for augmentation of the alveolar ridge and periodontal defects and tooth extraction sockets.
[0065] The following examples are illustrative-of the present invention and are not limiting in any manner. Modifications, variations and minor enhancements are contemplated and are within the scope of the present invention.
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WO 2005/025595 PCT7US2004/02956
EXAMPLES
[0066] All materials utilized in these examples are pharmaceutical grade. The calcium phosphate material was the commercially available bone substitute material (hereinafter "a-BSM") sold under the tradename CEREDEX™ by Etex Corporation, 38 Sydney Street, Cambridge, MA 02139 and described in detail in U.S Patent Nos. 5,683,461, 6,117,456 and 6,214,368 and PCT WO 98/16209. The osteogenic protein utilized as an active agent was recombinant human bone morphogenetic protein-2 (rhBMP-2). The production and characterization of rh BMP 2 is well known. Wang, et ai., Proc. Nat'i Acad. Sci. U.S.A., vol. 87, pp. 2220-2224 (1990).
Example 1 - Formulation
[0067] [66] Various injectable solid rods (A-E) of a-BSM, rhBMP-2, and-one
of carboxymethylcellulose (CMC), polyvinylpyrrolidone (PVP) and dextran sulfate were prepared. Dry powders of each component were combined in a sterile mixing bulb and reconstituted into a stiff putty by the addition of a a glycine-based aqueous buffer having a pH of about 4.5 and comprising 5 mmol L-glutamic acid, 2.5% glycine, 0.5% sucrose, 5 mmol NaCI and 0.01% polysorbate 80 at a specified liquid (ml) to powder (g) ratio (L/P) and kneaded for 1 minute to form a paste. The amounts of each ingredient, as well as the particular L/P ratios employed, were as set forth in Table 1 below.
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WO 2005/025595 PCT/US2004/029560

Table 1.
ROD a-BSM (wr%) RHBMP-2 (WT%) L/P RATIO
CMC (WT%)
A 68 17 15 I .O
B 56 29 15 I .2
C 45 4O 15 I .5
PVP (WT%)
D 45 4O 15 O.6
DEXTRAN SULFATE (WT%)
E 45 4O 15 O.6
[0068] Each paste was loaded into a syringe and extruded to form cylindrical rods having a diameter of about 1 mm and a length of about 5 cm. These 5 cm rods were then cut to desired lengths for testing below.
[0069] Following molding, the rods were hardened into a solid mass under simulated in vivo conditions, i.e., 37° incubator, overnight. The hardening process could be delayed for several hours by storing the rods at a cooler temperature, i.e., about 4° C.
[0070] The theoretical rhBMP-2 dose of each of rods A-E was 150 micrograms rhBMP-2 per milligram rod. All rods appeared to be-smooth, dense, cohesive and non-brittle and were injectable through a 16-gauge needle.
V . 27 ;

WO 2005/025595 PCT/US2004/029560
Example 2 - In Vitro Characterization
[0071] A correlation was observed between additive content and particle formation. Upon submersion in the glycine-based aqueous buffer described in Example 1 for 14 days, rods containing higher % additive (40% CMC, 40% PVP, or 40% dextran sulfate, i.e., rods C-E, respectively) dispersed into smaller particles faster, i.e., within 1 day, than those with medium (29% CMC, i.e., rod B) and low (17% CMC, i.e., rod A) additive contents. Rods containing low additive contents (17% CMC, i.e., rod A) maintained their rod-like structure much longer, i.e., on the order of days to weeks, than their higher CMC counterparts.
[0072] In addition, in vitro release kinetics of rhBMP-2 from ?-BSM rods containing either 17 % (rod A), 29 % (rod B), or 40 % (rod C) CMC was performed using 125l-rhBMP-2 as a tracer. Similar to gross observations, rods-containing higher % CMC released rhBMP-2 faster than those with lower % CMC as shown in Figure 1.
Example 3 - Rat Biocompatibilitv and Efficacy Study
[0073] Injectable rod-shaped calcium phosphate-based compositions /prepared as described in Example 1) were evaluated for biocompatibility and efficacy, both intraosseous and ectopic, in a rat model.
[0074] Intraosseous core defects were surgically established in the distal femurs of six male CD rats. The rats were divided into two groups (A and B) of three. One limb from each rat received a 2 mm segment test rod {calcium phosphate/rhBMP-2/excipient), while the contralateral limb received a 2 mm segment control rod (calcium phosphate/excipient). The rats ofGroup A received rod A (68/17/15 (w/w) % ACP/CMC/rhBMP-2) of Example 1 as the test rod, while the rats of Group B received rod B(56/29/15 (w/w) % ACP/CMC/rhBMP-2) of Example 1
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WO 2005/025595 PCT/US2004/029560
as the test rod. In addition, one 1O mm rod was implanted subcutaneously lateral to the midline of the ventral thorax region, with one side receiving a test rod and the other side receiving a control rod. Here again, the rats of Group A received rod A (68/17/15 (w/w) % ACP/CMC/rhBMP-2) of Example 1 as the test rod, while the rats of Group B received rod B (56/29/15 (w/w) % ACP/CMC/rhBMP-2) of Example 1 as the test rod.
[0075] Animals were sacrificed at 2 weeks for outcome assessments consisting of faxitron radiographs and histomorphometry. Radiographs and histology of the 2 week distal femur expiants indicated an overall increased bony callus formation in the rhBMP-2 treated side compared to placebo. In particular, higher CMC containing rods (B) formed medium to large bony calluses compared to lower CMC containing rods (A) which formed small to medium size calluses. Radiographs and histology of the subcutaneous implants also indicated bone formation in the rhBMP-2 treated side compared to placebo. In particular, higher CMC containing rods (B) formed a more robust ectopic bone response compared to lower CMC containing rods (A). Good biocompatibility was observed in all groups with no inflammatory response to the implants.
Example 4 - Rabbit Intraosseous Local Biodistribution Study
[0076] Rods A-D from Example 1 were injected percutaneously into rabbit distal femur intraosseous spaces (n=4 sites per group) using a 14-gauge catheter with a 16-gauge needle). In particular, 10 mm rods A-D containing approximately µ Ci 125!-rhBMP-2 were employed. The amount of radioactivity in the synthetic rods was measured before injection using a Capintec dose calibrator. Local rhBMP-2 retention was monitored over 4 weeks using gamma scintigraphy. Local in-vivo biodistribution data suggested a sustained release of rhBMP-2 from the rods and
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WO 2005/025595 PCT7US2004/029560
that higher % additive containing rods exhibited a faster in vivo release than their lower % additive counterparts as shown in Figure 2.
Example 5 - Formulation
[0077] Hardenable pastes containing either 1 mg rhBMP-2 per mL of paste or 4.5 mg rhBMP-2 per mL of paste were formulated by adding 2 mL of appropriately concentrated solutions of rhBMP-2 in the glycine-based aqueous buffer of Example 1 to 2.5 g ? -BSM. The mixtures were kneaded in sterile bulbs for 1 minute to form hardenable paste.
Example 6 - Cynomoloqous Monkey- Intraosseous Injection of Paste
[0078] The 1 mg per mL and 4.5 mg per mL hardenable pastes of Example 5 were extracted from the sterile bulbs using a 3 mL syringe fitted with an 18-gauge needle and then injected under fluoroscopic control into various intraosseous sites of cynomologous monkeys. Sites included distal femur, proximal femur, distal radius, proximal tibia. After 1 month, significant new bone was observed at the local sites where the rhBMP-2 was injected.
[0079] While the invention has been described in terms of preferred embodiments and specific examples, those skilled in the art will recognize through routine experimentation that various changes and modifications can-be made without departing from the spirit and scope of the invention. Thus, the invention should be understood as not being limited by the foregoing detailed description, but as being defined by the appended claims and their equivalents.
30

WE CLAIM :
1. A composition for injectable delivery of osteogenic proteins comprising an
osteogenic protein and a calcium phosphate material selected from the group consisting of
amorphous apatitic calcium phosphate and poorly crystalline apatitic calcium phosphate,
wherein said composition is in the form of a hardenable paste that hardens into a solid form
at 37°C.
2. The composition as claimed in claim 1, wherein the osteogenic protein is selected
from the group consisting of members of the bone morphogenetic protein (BMP) family.
3. The composition as claimed in claim 1, wherein the osteogenic protein is selected
from the group consisting of BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-10, BMP-12,
BMP-13, and MP52.
4. The composition as claimed in claim 1, wherein the osteogenic protein is BMP-2.
5. The composition as claimed in claim 1, wherein the osteogenic protein is BMP-
12.
6. The composition as claimed in claim 1, wherein the osteogenic protein is BMP-
13.
7. The composition as claimed in claim 1, wherein the osteogenic protein is MP52.
8. The composition as claimed in claim 1, wherein the osteogenic protein is
heterodimeric.
9. The composition as claimed in claim 1, wherein the osteogenic protein is present
in an amount ranging from 0.0I % to 2% by weight of the composition.
10. The composition as claimed in claim 1, wherein the osteogenic protein is present
in an amount ranging from 0.03% to 1 % by weight of the composition.
11. The composition as claimed in claim 1, comprising a bone resorption inhibitor.
12. The composition as claimed in claim 11, wherein the bone resorption inhibitor is a
bisphosphonate.
13. The composition as claimed in claim 12, wherein the bisphosphonate is selected
from the group consisting of alendronate, cimadronate, clodronate, EB 1053, etidronates,
ibandronate, neridronate, olpadronate, pamidronate, risedronate, tiludronate, YH 529.
zoledronate, and pharmaceutically acceptable salts, esters, and acids.
14. The composition as claimed in claim 1, wherein the calcium phosphate material
comprises poorly crystalline apatitic calcium phosphate.
15. The composition as claimed in claim 14, wherein the poorly crystalline apatitic
calcium phosphate has a calcium-to-phosphate ratio of less than 1.5.
16. The composition as claimed in claim 15, wherein the poorly crystalline apatitic
calcium phosphate has a calcium-to-phosphate ratio of 1.4.
17. The composition as claimed in claim 1, wherein the calcium phosphate material
selected from the group consisting of amorphous apatitic calcium phosphate and poorly
crystalline apatitic calcium phosphate is present in an amount ranging from 30% to 70% by
weight of the composition.
18. The composition as claimed in claim 17, wherein the calcium phosphate material
selected from the group consisting of amorphous apatitic calcium phosphate and poorly
crystalline apatitic calcium phosphate is present in an amount ranging from 45% to 55% by
weight of the composition.
19. A method for preparing a composition for injectable delivery of osteogenic
proteins comprising mixing a dry form of the calcium phosphate material selected from the
group consisting of amorphous apatitic calcium phosphate and poorly crystalline apatitic
calcium phosphate with an aqueous buffer containing an osteogenic protein to form a paste,
wherein said paste hardens into a solid form at 37°C.
20. The method as claimed in claim 19, wherein the osteogenic protein is selected
from the group consisting of members of the bone morphogenetic protein family.
21. The method as claimed in claim 19, wherein the osteogenic protein is selected
from the group consisting of BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-10, BMP-12,
BMP-13, and MP52.
22. The method as claimed in claim 19, wherein the osteogenic protein is BMP-2.
23. The method as claimed in claim 19, wherein the osteogenic protein is BMP-12.
24. The method as claimed in claim 19, wherein the osteogenic protein is BMP-13.
25. The method as claimed in claim 19, wherein the osteogenic protein is MP52.
26. The method as claimed in claim 19, wherein the osteogenic protein is present in
the paste in an amount ranging from 0.01% to 2% by weight of the paste.
27. The method as claimed in claim 26, wherein the osteogenic protein is present in
the paste in an amount ranging from 0.03% to 1 % by weight ofthe paste.
28. The method as claimed in claim 19, wherein the composition comprises a bone
resorption inhibitor and wherein a dry form of the bone resorption inhibitor is mixed in the
paste.
29. The method as claimed in claim 28, wherein the bone resorption inhibitor is a
bisphosphonate.
30. The method as claimed in claim 29, wherein the bisphosphonate is selected from
the group consisting of alendronate, cimadronate, c1odronate, EB 1053, etidronates,
ibandronate, neridronate, olpadronate, pamidronate, risedronate, tiludronate, YH 529,
zoledronate, and pharmaceutically acceptable salts, esters, and acids.
31. The method as claimed in claim 19, wherein the calcium phosphate material
comprises a poorly crystalline apatitic calcium phosphate.
32. The method as claimed in claim 31, wherein the poorly crystalline apatitic
calcium phosphate has a calcium-to-phosphaee ratio of less than 1.5.
33. The method as claimed in claim 32, wherein the poorly crystalline apatitic
calcium phosphate has a calcium-to-phosphate ratio of 1.4.
34. The method as claimed in claim 19. wherein an amount of calcium phosphate
material selected from the group consisting of amorphous apatitic calcium phosphate and
poorly crystalline apatitic calcium phosphate ranging from 30% to 70% by weight of the
composition is mixed with the aqueous buffer.
35. The method as claimed in claim 34, wherein an amount of calcium phosphate
material ranging from 45% to 55% by weight of the composition is mixed with the aqueous
buffer.
36. The method as claimed in claim 19, wherein the aqueous buffer is selected from
the group consisting of phosphate-buffered saline, saline, and glycine-based buffers.
37. The method as claimed in claim 19, wherein the ratio of aqueous buffer to calcium
phosphate material ranges from 0.5:1 to 2:1.
38. A pharmaceutical composition for treating a mammal having a bone defect
comprising an effective amount of the composition for injectable delivery of osteogenic
proteins as claimed in claim 1.
39. A pharmaceutical composition for treating a mammal having a bone defect
comprising:
(a) an effective amount of the composition for injectable delivery of osteogenic proteins as
claimed in claim 1; and
(b) an effective amount of a bone resorption inhibitor.

Documents:

00915-kolnp-2006 correspondence.pdf

00915-kolnp-2006 form-18.pdf

00915-kolnp-2006- pct form .pdf

00915-kolnp-2006-abstract.pdf

00915-kolnp-2006-assignment-1.1.pdf

00915-kolnp-2006-assignment.pdf

00915-kolnp-2006-claims.pdf

00915-kolnp-2006-correspondence other.pdf

00915-kolnp-2006-correspondence others-1.1.pdf

00915-kolnp-2006-description complete.pdf

00915-kolnp-2006-drawings.pdf

00915-kolnp-2006-form 1.pdf

00915-kolnp-2006-form 13.pdf

00915-kolnp-2006-form 3-1.1.pdf

00915-kolnp-2006-form 3.pdf

00915-kolnp-2006-form 5.pdf

00915-kolnp-2006-international publication.pdf

00915-kolnp-2006-international search report.pdf

00915-kolnp-2006-priority document-1.1.pdf

00915-kolnp-2006-priority document.pdf

915-KOLNP-2006-(29-03-2012)-CORRESPONDENCE.pdf

915-KOLNP-2006-(29-03-2012)-OTHERS.pdf

915-KOLNP-2006-(29-03-2012)-PA.pdf

915-KOLNP-2006-ABSTRACT.pdf

915-KOLNP-2006-AMANDED CLAIMS.pdf

915-KOLNP-2006-CANCELLED PAGES.pdf

915-KOLNP-2006-DESCRIPTION (COMPLETE).pdf

915-KOLNP-2006-DRAWINGS.pdf

915-KOLNP-2006-FORM 1.pdf

915-KOLNP-2006-FORM 13.pdf

915-KOLNP-2006-FORM 3.pdf

915-KOLNP-2006-OTHERS.pdf

915-KOLNP-2006-PETETION UNDER RULE 137.pdf

915-KOLNP-2006-PETETION UNDER RULE137.pdf

915-KOLNP-2006-PETETION UNDER RULE137.tif.pdf

915-KOLNP-2006-REPLY TO EXAMINATION REPORT.pdf


Patent Number 243270
Indian Patent Application Number 915/KOLNP/2006
PG Journal Number 41/2010
Publication Date 08-Oct-2010
Grant Date 30-Sep-2010
Date of Filing 12-Apr-2006
Name of Patentee WYETH
Applicant Address 5 GIRALDA FARMS, MADISON, NJ 07940
Inventors:
# Inventor's Name Inventor's Address
1 LI REBECCA 14 SHIRE LANE, BEDFORD, MA 01730
2 KIM HYUN 14 COT HILL ROAD, BEDFORD, MA 01730
3 SEEHERMAN HOWARD 46B TROWBRIDGE STREET, CAMBRIDGE MA 02138
PCT International Classification Number A61K 38/18
PCT International Application Number PCT/US2004/029560
PCT International Filing date 2004-09-10
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 60/502,493 2003-09-12 U.S.A.